TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419471.2.6
(1648 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY560003.1| Medicago sativa S-adenosylmethionine synthas... 840 0.0
gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular tr... 368 e-102
gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular tr... 249 8e-067
gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular tr... 234 2e-062
gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular tr... 231 2e-061
gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted... 108 2e-024
>gb|AY560003.1| Medicago sativa S-adenosylmethionine synthase mRNA, complete cds
Length = 1173
Score = 840 bits (1827), Expect = 0.0
Identities = 346/390 (88%), Positives = 361/390 (92%)
Frame = +3 / +1
Query: 186 VDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTK 365
++TFLFTSESVNEGHPDKLCDQ+SDAVLDACL +D DSKVACETCTKTNMVMVFGEITTK
Sbjct: 1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDVDSKVACETCTKTNMVMVFGEITTK 180
Query: 366 ANVDYEKIVRETCRNIGFVSNDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGA 545
A VDYEKIVR+TCR IGFVS+DVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPEEIGA
Sbjct: 181 AKVDYEKIVRDTCRKIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGA 360
Query: 546 GDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYRNEG 725
GDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEY N+
Sbjct: 361 GDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNDN 540
Query: 726 GAMVPIRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEQYLDEKTIFHLNPSGRFVI 905
GAMVP+RVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPE+YLD KTIFHLNPSGRFVI
Sbjct: 541 GAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDSKTIFHLNPSGRFVI 720
Query: 906 GGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYVARQAAKSIVASGLAR 1085
GGPHGDAGLTGRKIIIDTY KDPTKVDRSGAY+ RQAAKSIVASGLAR
Sbjct: 721 GGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVASGLAR 900
Query: 1086 RAIVQVSYAIGVPEPLSVFVDTYGTGAIPDKEILKIVKENFDFRPGMIIINLDLKKGGNG 1265
R IVQVSYAIGVPEPLSVFVDTYGTG IPDKEIL IVK+NFDFRPGMI INLDL +GGNG
Sbjct: 901 RCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKQNFDFRPGMISINLDLLRGGNG 1080
Query: 1266 RYLKTAAYGHFGRDDPDFTWEVVKPLKSEK 1355
R+LKTAAYGHFGR+D DFTWEVVKPLK EK
Sbjct: 1081 RFLKTAAYGHFGREDADFTWEVVKPLKWEK 1170
Score = 444 bits (964), Expect(3) = e-151
Identities = 205/257 (79%), Positives = 216/257 (84%)
Frame = -1 / -2
Query: 958 VSMMILRPVRPASP*GPPMTKRPDGLRWKMVFSSRYCSGMTGLMTCSFRSAAISSLVTVS 779
VSMMI PVRPASP*GPPMTKRP+G + KMV SRY SG+TG +TCS RSAAISSLVTVS
Sbjct: 773 VSMMIFLPVRPASP*GPPMTKRPEGFK*KMVLESRYFSGITGFITCSLRSAAISSLVTVS 594
Query: 778 SCWVEMRTVWTRMGTMAPPSLRYSTVTWVFPSGLSQGQVPFLRTSVRRAPSLVARTWLRG 599
SC VEM TVWTR GTMAP SL YSTVT V PSGL+QG VPFLRTSVRRAPSLVARTWL+G
Sbjct: 593 SC*VEMSTVWTRTGTMAPLSL*YSTVT*VLPSGLNQGHVPFLRTSVRRAPSLVARTWLKG 414
Query: 598 INSGVSSVAYPNMCPWSPAPISSGRLVKWPCTP*AISGDCCSMFTSTLQWSASSPTSFDT 419
INSGVSSVA PNM PWSPAPISSG V+ PCTP*AISG CCSM T TLQ SAS PTS +T
Sbjct: 413 INSGVSSVA*PNM*PWSPAPISSGLFVR*PCTP*AISGLCCSMLTRTLQLSASRPTSSET 234
Query: 418 KPMLRQVSLTIFS*STLALVVISPKTMTMLVLVQVSQATLLSGSSARQASRTASETWSQS 239
P+ QVS TIFS*STLALVVISPKT+TMLVLVQVS ATLLS S +RQAS TASE SQS
Sbjct: 233 NPIFLQVSRTIFS*STLALVVISPKTITMLVLVQVSHATLLSTSCSRQASSTASEI*SQS 54
Query: 238 LSGCPSFTDSEVKRNVS 188
LSG PSFTDSEV R VS
Sbjct: 53 LSG*PSFTDSEVNRKVS 3
Score = 210 bits (454), Expect(4) = 1e-088
Identities = 96/173 (55%), Positives = 129/173 (74%)
Frame = -3 / -1
Query: 719 IAVLDCHLGLPIRPEPGAGSVLADLGETST*LGGKDMAEGHQLRGLIGRIPEHVSLVTSS 540
I +L+ L L IR +P A ++LA+L ETST LG K+MA+ HQ R LI I +HV+LVTS+
Sbjct: 534 IVILNSDLSLTIRSQPRACTILANLSETSTKLGCKNMAQRHQFRSLISSIAKHVTLVTST 355
Query: 539 NLLGALGEVAMHTLSNIRGLLLNVHEHLAVVGVKPNIV*HKTNVAAGLPDNLLVVNIGLG 360
NLL + ++ M+TLSNIR LLLNV ++LAVV +K I+ +K N++ G+ N L+VN+ LG
Sbjct: 354 NLLRSFCKMTMYTLSNIRTLLLNVDKNLAVVSIKTYIIRNKPNLSTGITHNFLIVNLSLG 175
Query: 359 GDLTKDHDHVGLGAGLTSNLAVRVLSKASVKNGI*DLVTELVRVSLVHRLRGE 201
DLTKDH+HVGL A LT NLAV VL KAS+ + I DL+T+LV ++L+H LRGE
Sbjct: 174 CDLTKDHNHVGLSASLTCNLAVYVLFKASI*HSIRDLITKLVGMTLIHGLRGE 16
Score = 205 bits (442), Expect(3) = 4e-069
Identities = 89/164 (54%), Positives = 104/164 (63%)
Frame = -2 / -3
Query: 693 SSHQA*ARGRFRSCGPR*DEHLAWWQGHG*GASTQGSHRSHTRTCVPGHQLQSPRGAW*S 514
S HQ +G + SC P+*DEH AW Q HG AS Q SH+ H++TC PGHQ QSP+ *
Sbjct: 508 SYHQVSTKGMYHSCEPQ*DEHQAWLQEHGSKASIQESHQ*HSQTCDPGHQHQSPQVFL*D 329
Query: 513 GHAHPEQYQGTAAQCSRAPCSGRRQAQHRLTQNQCCGRSP*QSSRSQHWPWW*SHQRP*P 334
H HPEQYQ +AAQC + PCS + Q H Q Q R Q S SQ PW *SHQRP*P
Sbjct: 328 DHVHPEQYQDSAAQC*QEPCSCQHQDLHHQKQTQSFYRYHAQFSHSQP*PWL*SHQRP*P 149
Query: 333 CWSWCRSHKQPCCQGPQQGKRQERHLRPGHRACQGVPRSQTPR* 202
CWS C+SH QPCC P QGK +H R H+AC+ P S+T R*
Sbjct: 148 CWS*CKSHMQPCCLRPVQGKHLAQHQRFDHKACRDDPHSRTQR* 17
Score = 133 bits (285), Expect(4) = 9e-091
Identities = 65/123 (52%), Positives = 80/123 (65%)
Frame = +2 / +3
Query: 194 IPLHLGVCERGTP*QAL*PGLRCRS*RLPC*GP*QQGCL*DLHQDQHGHGLW*DHHQGQC 373
+ +HL V E G+ QAL* L C + LP G QQGC+*DLH DQHG+GLW*DH+QG
Sbjct: 9 LSIHL*VRE*GSSRQAL*SNL*CCARCLP*TGRRQQGCM*DLH*DQHGYGLW*DHNQG*G 188
Query: 374 *LREDCQGDLPQHWFCVKRCWA*RRPLQGAREH*AAVP*YCSGCAWPLHQAPRGDWSW*P 553
*L E+C L + W C C +* LQG+ +H*AA *YCSGC W ++ GDW W*P
Sbjct: 189 *L*ENCA*YL*KDWVCF**CRS*C*QLQGSCQH*AAES*YCSGCTWSSYKKT*GDWCW*P 368
Query: 554 GTH 562
G+H
Sbjct: 369 GSH 377
Score = 130 bits (278), Expect(4) = 9e-091
Identities = 54/104 (51%), Positives = 65/104 (62%)
Frame = +2 / +3
Query: 782 DSDQ**DRC*PEGACHQACHP*AVP*REDHLPP*PIWPLCHWWTSRRCWPHWPQDHH*HL 961
D Q**+ C* + AC++AC * VP* +DH P WP HW +SR CW HW +DHH*HL
Sbjct: 597 DCYQ**NCC*SQRACYKACDS*EVP*FQDHFSLEPFWPFRHWRSSR*CWSHW*EDHH*HL 776
Query: 962 RWLGSPWWWRFLRQGPNQG*PQRSLCRKAGCQEHRRQRPCSPRH 1093
WLG WWW FLR+ PN G* + SL +A C EH + C H
Sbjct: 777 WWLGCTWWWCFLRKRPN*G*QEWSLHCEASC*EHCCKWTCKEVH 908
Score = 95.9 bits (203), Expect(3) = 4e-022
Identities = 63/128 (49%), Positives = 65/128 (50%)
Frame = +1 / +2
Query: 310 VRPAPRPTWSWSLVRSPPRPMLTTRRLSGRPAATLVLCQTMLGLTPTTARCXXXXXXXXX 489
VR A RPTW WSLVRS PR LT R+L P L L M L TTAR
Sbjct: 125 VRLALRPTWLWSLVRSQPRLRLTMRKLCVIPVERLGLFLMM*VLMLTTARFLSTLSSRVL 304
Query: 490 XXXRVCMATSPSAPRRLELVTRDTCSGMRPMRPLS*CPSAMSLPPS*VLVSPRSARTEPA 669
RV M RRL LVTR TC M MR L+*C AM L PS VLVS R AR A
Sbjct: 305 ILLRVYMVILQKDLRRLVLVTRVTCLAMLLMRLLN*CL*AMFLQPSLVLVSLRFARMVHA 484
Query: 670 PGSGLMGR 693
G LM R
Sbjct: 485 LG*DLMVR 508
Score = 89.5 bits (189), Expect(3) = e-151
Identities = 38/46 (82%), Positives = 42/46 (91%)
Frame = -1 / -2
Query: 1228 IMPGLKSKFSLTILRISLSGIAPVPYVSTNTERGSGTPMA*DTWTM 1091
IMPGLKSKF TILRISLSGI PVPYVSTNT++GSGTP+A*+T TM
Sbjct: 1043 IMPGLKSKFCFTILRISLSGIFPVPYVSTNTDKGSGTPIA*ETCTM 906
Score = 76.2 bits (160), Expect(4) = 1e-088
Identities = 32/59 (54%), Positives = 42/59 (71%)
Frame = -3 / -1
Query: 1049 LPCDIGSAAVNLGWVLAGESATTMGSPATVGVNDDLAASEASIAVRSTNDKAARWVKVE 873
LP ++ S VNL WV + ES TTM +P T+ VNDDL SE SI VR++ND+ AR V+V+
Sbjct: 864 LPHNVSSTPVNLSWVFS*ESTTTMCTPTTISVNDDLPTSETSITVRTSNDETARRVQVK 688
Score = 70.3 bits (147), Expect(4) = 9e-091
Identities = 33/63 (52%), Positives = 41/63 (65%)
Frame = +2 / +3
Query: 1166 DPRQGDPQDCQGELRFQAWHDHHQP*PQERRQRALPQDGGLRPLWKGRP*LHLGGGEATQ 1345
DP G+PQ C+ EL FQAWHD +QP* E Q + +D L W GR *LH+G GEA+
Sbjct: 981 DP**GNPQYCEAEL*FQAWHDLYQP*SLEGWQWQVLEDCCLWTFW*GRC*LHMGSGEAS* 1160
Query: 1346 VGE 1354
+GE
Sbjct: 1161 MGE 1169
Score = 67.0 bits (140), Expect(4) = 1e-088
Identities = 28/77 (36%), Positives = 43/77 (55%)
Frame = -3 / -1
Query: 1337 LHHLPGEVRVVPSKVAVGRRLEVAPVAAFLEVKVDDDHARPEIXXXXXXXXXXXVGDRAG 1158
LHH P EV + P+K+++ L+ +A E+KVD DHARPEI + D
Sbjct: 1152 LHHFPCEVSIFPTKMSISSSLQEPAIATPQEIKVDRDHARPEIKVLLHNIEDFLIRDLPC 973
Query: 1157 AVRVDEHGERLGHADGV 1107
+ V++HG+RL H + +
Sbjct: 972 TICVNKHGQRLRHTNSI 922
Score = 55.1 bits (114), Expect(3) = 4e-069
Identities = 27/50 (54%), Positives = 31/50 (62%)
Frame = -2 / -3
Query: 1062 CSWQPALRHRLRCGQPWLGPCRRKRHHHGLPSHRRCQ**SCGQ*GQHRRE 913
CS Q A + +L QP LG RK HHH P+H +CQ**S Q* QH RE
Sbjct: 877 CS*QLASQCKLHSCQP*LGLFLRKHHHHVHPNHHKCQ**SSYQ*DQHHRE 728
Score = 53.8 bits (111), Expect(4) = 9e-091
Identities = 22/42 (52%), Positives = 26/42 (61%)
Frame = +2 / +3
Query: 605 QPCPCHQARCSSHRGPQERNLPLAQA*WEDPGDSRVPQ*GWC 730
+PC C+QA CSSH G QE +PL + *W D V Q* WC
Sbjct: 420 EPCSCNQAWCSSH*GSQEWYMPLVET*W*DSSHC*VLQ*QWC 545
Score = 45.5 bits (93), Expect(3) = e-151
Identities = 21/26 (80%), Positives = 21/26 (80%)
Frame = -1 / -2
Query: 1354 FSDLSGFTTSQVKSGSSLPKWP*AAV 1277
FS L GFTTS VKS SSLPK P*AAV
Sbjct: 1169 FSHLRGFTTSHVKSASSLPKCP*AAV 1092
Score = 43.2 bits (88), Expect(3) = 4e-069
Identities = 25/48 (52%), Positives = 29/48 (60%)
Frame = -2 / -3
Query: 1353 SPT*VASPPPR*SQGRPFQSGRRPPS*GSARCRLS*GQG***SCQA*N 1210
SP * ASP P *SQ P+Q+ + S + C S QG** SCQA*N
Sbjct: 1168 SPI*EASPLPM*SQHLPYQNVHKQQSSRTCHCHPSRDQG**RSCQA*N 1025
Score = 27.2 bits (53), Expect(3) = 4e-022
Identities = 13/23 (56%), Positives = 14/23 (60%)
Frame = +1 / +2
Query: 964 VAGEPMVVALSPARTQPRLTAAE 1032
V G MVV LS +TQ RLT E
Sbjct: 779 VVGVHMVVVLSQEKTQLRLTGVE 847
Score = 26.3 bits (51), Expect(4) = 1e-088
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = -2 / -3
Query: 852 TAQG*QA**HAPSGQQRSHHW 790
T+Q QA *HA QQ+ HHW
Sbjct: 667 TSQESQAL*HAL*DQQQFHHW 605
Score = 21.7 bits (41), Expect(3) = 4e-022
Identities = 9/14 (64%), Positives = 10/14 (71%)
Frame = +1 / +2
Query: 1297 LEGTTLTSPGRW*S 1338
L G LTS G+W*S
Sbjct: 1112 LVGKMLTSHGKW*S 1153
>gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 600
Score = 368 bits (797), Expect = e-102
Identities = 148/161 (91%), Positives = 157/161 (97%)
Frame = +3 / +2
Query: 183 AVDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITT 362
A +TFL+TSESVNEGHPDKLCDQ+SDAVLDACL +DPDSKVACETCTKTNMVMVFGEITT
Sbjct: 116 ATETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITT 295
Query: 363 KANVDYEKIVRETCRNIGFVSNDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIG 542
KANVDYEKIVR TCRNIGFVS+DVGLDAD+CKVLVNIEQQSPDIAQGVHGH T+RPEEIG
Sbjct: 296 KANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTERPEEIG 475
Query: 543 AGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGT 665
AGDQGHMFGYATDETPELMPL+HVLATKLGA+LTEVRKNGT
Sbjct: 476 AGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGT 598
Score = 276 bits (598), Expect = 4e-075
Identities = 127/167 (76%), Positives = 136/167 (81%)
Frame = -1 / -1
Query: 664 VPFLRTSVRRAPSLVARTWLRGINSGVSSVAYPNMCPWSPAPISSGRLVKWPCTP*AISG 485
VPFLRTSV AP+LVARTW++GI+SGVSSVAYPNM P SPAPISSG V+WP TP*AISG
Sbjct: 597 VPFLRTSVSLAPNLVARTWVKGISSGVSSVAYPNM*P*SPAPISSGLSVRWP*TP*AISG 418
Query: 484 DCCSMFTSTLQWSASSPTSFDTKPMLRQVSLTIFS*STLALVVISPKTMTMLVLVQVSQA 305
CCSM T TLQ SAS PTS +TKPM V TIFS*ST A VVISPKT+TMLV VQVS A
Sbjct: 417 LCCSMLTRTLQLSASRPTSSETKPMFLHVLRTIFS*STFAFVVISPKTITMLVFVQVSHA 238
Query: 304 TLLSGSSARQASRTASETWSQSLSGCPSFTDSEVKRNVSTAAIFFDL 164
TLLSGS +RQASRTASE WSQSLSGCPSFTDS+V R VS A DL
Sbjct: 237 TLLSGSCSRQASRTASEIWSQSLSGCPSFTDSDVYRKVSVAIFNQDL 97
Score = 187 bits (403), Expect = 3e-048
Identities = 90/152 (59%), Positives = 111/152 (73%)
Frame = -3 / -3
Query: 662 SVLADLGETST*LGGKDMAEGHQLRGLIGRIPEHVSLVTSSNLLGALGEVAMHTLSNIRG 483
SVLA+ E S+*LG K+M H+LR IG IPEHV+L+TS+N L + ++AM+TLSNIR
Sbjct: 595 SVLANFSELSS*LGCKNMG*RHKLRCFIGSIPEHVTLITSTNFLRSFCKMAMNTLSNIRT 416
Query: 482 LLLNVHEHLAVVGVKPNIV*HKTNVAAGLPDNLLVVNIGLGGDLTKDHDHVGLGAGLTSN 303
LL NV + AVV +K NIV*+KT V A + DNL +VNI DLTKDH+HVGL A T N
Sbjct: 415 LLFNVDKDFAVVSIKTNIV*NKTYVPACVTDNLFIVNICFCCDLTKDHNHVGLCASFTCN 236
Query: 302 LAVRVLSKASVKNGI*DLVTELVRVSLVHRLR 207
AV +L +AS+KNGI DLV +LV VSLVHR R
Sbjct: 235 FAVWILLEASIKNGIRDLVAKLVGVSLVHRFR 140
Score = 171 bits (367), Expect = 3e-043
Identities = 80/150 (53%), Positives = 89/150 (59%)
Frame = -2 / -2
Query: 663 FRSCGPR*DEHLAWWQGHG*GASTQGSHRSHTRTCVPGHQLQSPRGAW*SGHAHPEQYQG 484
FRSC +* L W Q HG A Q HR HTRTC HQ Q P+ * GH H EQYQ
Sbjct: 596 FRSCELQ*A*LLTWLQEHGLKA*AQVFHR*HTRTCDLDHQHQFPQVFL*DGHEHLEQYQD 417
Query: 483 TAAQCSRAPCSGRRQAQHRLTQNQCCGRSP*QSSRSQHWPWW*SHQRP*PCWSWCRSHKQ 304
+ QC + CS + Q QHRL QN C QS SQH *SHQRP*PCWS C+ H Q
Sbjct: 416 SVVQC*QGLCSCQHQDQHRLKQNLCSCMCYGQSFHSQHLLLL*SHQRP*PCWSLCKFHMQ 237
Query: 303 PCCQGPQQGKRQERHLRPGHRACQGVPRSQ 214
CC +GK QERH R G +AC+GVPRSQ
Sbjct: 236 LCCLDLARGKHQERHQRSGRKACRGVPRSQ 147
Score = 158 bits (340), Expect(2) = 3e-042
Identities = 64/125 (51%), Positives = 86/125 (68%)
Frame = +2 / +1
Query: 188 RHIPLHLGVCERGTP*QAL*PGLRCRS*RLPC*GP*QQGCL*DLHQDQHGHGLW*DHHQG 367
R+ P+H+ +CERGTP QAL P L CRS* LP QQ C+*+LH+DQHG+GLW*DH++
Sbjct: 121 RNFPIHI*ICERGTPRQALRPDL*CRS*CLPRARSRQQSCM*NLHKDQHGYGLW*DHNKS 300
Query: 368 QC*LREDCQGDLPQHWFCVKRCWA*RRPLQGAREH*AAVP*YCSGCAWPLHQAPRGDWSW 547
+C*L +DC + +H FC +RCW+* LQ +H* *YCS C+WP ++ G+W W
Sbjct: 301 KC*L*KDCP*HMQEHRFCFRRCWS*C*QLQSPCQH*TTES*YCSRCSWPSYRKT*GNWCW 480
Query: 548 *PGTH 562
* +H
Sbjct: 481 *SRSH 495
Score = 126 bits (270), Expect = 7e-030
Identities = 72/151 (47%), Positives = 82/151 (54%)
Frame = +1 / +3
Query: 211 SL*TRDTLTSSVTRSQMPFXXXXXXXXXXXXXXVRPAPRPTWSWSLVRSPPRPMLTTRRL 390
+L*TRDT TS TRS MPF V+ A RPTW WSLVRS + MLT +RL
Sbjct: 144 NL*TRDTPTSFATRSLMPFLMLASSKIQTAKLHVKLAQRPTWLWSLVRSQQKQMLTMKRL 323
Query: 391 SGRPAATLVLCQTMLGLTPTTARCXXXXXXXXXXXXRVCMATSPSAPRRLELVTRDTCSG 570
S A T VL QTML L TTA+ +V MA R+L LV + TCSG
Sbjct: 324 SVTHAGT*VLFQTMLVLMLTTAKSLSTLNNRVLILLKVFMAILQKDLRKLVLVIKVTCSG 503
Query: 571 MRPMRPLS*CPSAMSLPPS*VLVSPRSARTE 663
M PM+ LS C M L PS* L S + ARTE
Sbjct: 504 MLPMKHLSLCL*PMFLQPS*ELSSLKFARTE 596
Score = 29.0 bits (57), Expect(2) = 3e-042
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = +2 / +1
Query: 608 PCPCHQARCSSHRGPQERNL 667
PC C+Q R +H QERN+
Sbjct: 541 PCSCNQVRS*AH*SSQERNM 600
>gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 505
Score = 249 bits (538), Expect = 8e-067
Identities = 103/118 (87%), Positives = 109/118 (92%)
Frame = +3 / +3
Query: 1002 KDPTKVDRSGAYVARQAAKSIVASGLARRAIVQVSYAIGVPEPLSVFVDTYGTGAIPDKE 1181
KDPTKVDRSGAY+ RQAAKSIVA+GLARR IVQVSYAIGVPEPLSVFVD+YGTG IPDKE
Sbjct: 24 KDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKE 203
Query: 1182 ILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKSEK 1355
IL IVKE+FDFRPGMI INLDLK+GGN R+LKTAAYGHFGRDD DFTWEVVKPLK K
Sbjct: 204 ILNIVKESFDFRPGMISINLDLKRGGNNRFLKTAAYGHFGRDDADFTWEVVKPLKGGK 377
Score = 74.8 bits (157), Expect(2) = 6e-022
Identities = 34/46 (73%), Positives = 39/46 (84%)
Frame = -1 / -1
Query: 1228 IMPGLKSKFSLTILRISLSGIAPVPYVSTNTERGSGTPMA*DTWTM 1091
I+PGLKSK S T+LRISLSGI PVP STNT+ GSGTP+A*+T TM
Sbjct: 250 IIPGLKSKDSFTMLRISLSGIFPVP*ESTNTDNGSGTPIA*ET*TM 113
Score = 44.6 bits (91), Expect(2) = 6e-022
Identities = 21/26 (80%), Positives = 21/26 (80%)
Frame = -1 / -1
Query: 1354 FSDLSGFTTSQVKSGSSLPKWP*AAV 1277
F LSGFTTS VKS SSLPK P*AAV
Sbjct: 376 FPPLSGFTTSHVKSASSLPK*P*AAV 299
>gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 425
Score = 234 bits (506), Expect = 2e-062
Identities = 94/103 (91%), Positives = 100/103 (97%)
Frame = +3 / +2
Query: 183 AVDTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITT 362
A +TFL+TSESVNEGHPDKLCDQ+SDAVLDACL +DPDSKVACETCTKTNMVMVFGEITT
Sbjct: 116 ATETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITT 295
Query: 363 KANVDYEKIVRETCRNIGFVSNDVGLDADHCKVLVNIEQQSPD 491
KANVDYEKIVR TCRNIGFVS+DVGLDAD+CKVLVNIEQQSPD
Sbjct: 296 KANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPD 424
Score = 173 bits (372), Expect = 6e-044
Identities = 81/109 (74%), Positives = 85/109 (77%)
Frame = -1 / -3
Query: 490 SGDCCSMFTSTLQWSASSPTSFDTKPMLRQVSLTIFS*STLALVVISPKTMTMLVLVQVS 311
SG CCSM T TLQ SAS PTS +TKPM V TIFS*ST A VVISPKT+TMLV VQVS
Sbjct: 423 SGLCCSMLTRTLQLSASRPTSSETKPMFLHVLRTIFS*STFAFVVISPKTITMLVFVQVS 244
Query: 310 QATLLSGSSARQASRTASETWSQSLSGCPSFTDSEVKRNVSTAAIFFDL 164
ATLLSGS +RQASRTASE WSQSLSGCPSFTDS+V R VS A DL
Sbjct: 243 HATLLSGSCSRQASRTASEIWSQSLSGCPSFTDSDVYRKVSVAIFNQDL 97
Score = 124 bits (266), Expect = 3e-029
Identities = 52/96 (54%), Positives = 69/96 (71%)
Frame = +2 / +1
Query: 188 RHIPLHLGVCERGTP*QAL*PGLRCRS*RLPC*GP*QQGCL*DLHQDQHGHGLW*DHHQG 367
R+ P+H+ +CERGTP QAL P L CRS* LP QQ C+*+LH+DQHG+GLW*DH++
Sbjct: 121 RNFPIHI*ICERGTPRQALRPDL*CRS*CLPRARSRQQSCM*NLHKDQHGYGLW*DHNKS 300
Query: 368 QC*LREDCQGDLPQHWFCVKRCWA*RRPLQGAREH* 475
+C*L +DC + +H FC +RCW+* LQ +H*
Sbjct: 301 KC*L*KDCP*HMQEHRFCFRRCWS*C*QLQSPCQH* 408
Score = 120 bits (257), Expect = 4e-028
Identities = 58/95 (61%), Positives = 68/95 (71%)
Frame = -3 / -2
Query: 491 IRGLLLNVHEHLAVVGVKPNIV*HKTNVAAGLPDNLLVVNIGLGGDLTKDHDHVGLGAGL 312
IR LL NV + AVV +K NIV*+KT V A + DNL +VNI DLTKDH+HVGL A
Sbjct: 424 IRTLLFNVDKDFAVVSIKTNIV*NKTYVPACVTDNLFIVNICFCCDLTKDHNHVGLCASF 245
Query: 311 TSNLAVRVLSKASVKNGI*DLVTELVRVSLVHRLR 207
T N AV +L +AS+KNGI DLV +LV VSLVHR R
Sbjct: 244 TCNFAVWILLEASIKNGIRDLVAKLVGVSLVHRFR 140
Score = 119 bits (254), Expect = 1e-027
Identities = 51/93 (54%), Positives = 58/93 (62%)
Frame = -2 / -1
Query: 492 YQGTAAQCSRAPCSGRRQAQHRLTQNQCCGRSP*QSSRSQHWPWW*SHQRP*PCWSWCRS 313
YQ + QC + CS + Q QHRL QN C QS SQH *SHQRP*PCWS C+
Sbjct: 425 YQDSVVQC*QGLCSCQHQDQHRLKQNLCSCMCYGQSFHSQHLLLL*SHQRP*PCWSLCKF 246
Query: 312 HKQPCCQGPQQGKRQERHLRPGHRACQGVPRSQ 214
H Q CC +GK QERH R G +AC+GVPRSQ
Sbjct: 245 HMQLCCLDLARGKHQERHQRSGRKACRGVPRSQ 147
>gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 435
Score = 231 bits (499), Expect = 2e-061
Identities = 93/103 (90%), Positives = 99/103 (96%)
Frame = +3 / +1
Query: 189 DTFLFTSESVNEGHPDKLCDQVSDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKA 368
+TFLFTSESVNEGHPDKLCDQ+SDAVLDACL +DPDSK ACETCTKTNMVMVFGEITTKA
Sbjct: 127 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKGACETCTKTNMVMVFGEITTKA 306
Query: 369 NVDYEKIVRETCRNIGFVSNDVGLDADHCKVLVNIEQQSPDIA 497
NVDYEKIVR+TCR IGF+S+DVGLDAD CKVLVNIEQQSPDIA
Sbjct: 307 NVDYEKIVRDTCRTIGFISDDVGLDADKCKVLVNIEQQSPDIA 435
Score = 167 bits (360), Expect = 3e-042
Identities = 79/103 (76%), Positives = 83/103 (80%)
Frame = -1 / -2
Query: 496 AISGDCCSMFTSTLQWSASSPTSFDTKPMLRQVSLTIFS*STLALVVISPKTMTMLVLVQ 317
AISG CCSM T TL SAS PTS + PM+R VS TIFS*STLALVVISPKTMTMLVLV
Sbjct: 434 AISGLCCSMLTKTLHLSASRPTSSEMNPMVRHVSRTIFS*STLALVVISPKTMTMLVLVH 255
Query: 316 VSQATLLSGSSARQASRTASETWSQSLSGCPSFTDSEVKRNVS 188
VSQA LLSGS +RQAS TASE WS SLSG PSFTDS+V R VS
Sbjct: 254 VSQAPLLSGSCSRQASSTASEIWSHSLSG*PSFTDSDVNRKVS 126
Score = 122 bits (262), Expect = 9e-029
Identities = 51/93 (54%), Positives = 56/93 (60%)
Frame = -2 / -3
Query: 495 QYQGTAAQCSRAPCSGRRQAQHRLTQNQCCGRSP*QSSRSQHWPWW*SHQRP*PCWSWCR 316
QYQ +AAQC PC R Q QH Q CG +SS S WPW QRP*PC SWC
Sbjct: 433 QYQDSAAQC*PRPCICRHQDQHHQR*IQWCGMCHERSSHSLRWPWLLFLQRP*PC*SWCM 254
Query: 315 SHKQPCCQGPQQGKRQERHLRPGHRACQGVPRS 217
SH+ PCCQGP Q KR+ H R G AC+G PRS
Sbjct: 253 SHRHPCCQGPAQDKRRALHQRSGRTACRGDPRS 155
Score = 116 bits (248), Expect = 8e-027
Identities = 52/102 (50%), Positives = 70/102 (68%)
Frame = +2 / +3
Query: 191 HIPLHLGVCERGTP*QAL*PGLRCRS*RLPC*GP*QQGCL*DLHQDQHGHGLW*DHHQGQ 370
++ +++ + ERG+P QA+ P L C + RL GP*QQGCL*D+HQD HGHGLW +++QGQ
Sbjct: 129 NLSIYI*ISERGSPRQAVRPDL*CSARRLS*AGP*QQGCL*DMHQD*HGHGLWRNNNQGQ 308
Query: 371 C*LREDCQGDLPQHWFCVKRCWA*RRPLQGAREH*AAVP*YC 496
L ED +P HW + CW+* R +QG +H*AA *YC
Sbjct: 309 RRL*EDRS*HMPHHWIHL**CWS*CRQMQGLGQH*AAES*YC 434
Score = 115 bits (246), Expect = 1e-026
Identities = 56/97 (57%), Positives = 71/97 (73%)
Frame = -3 / -1
Query: 497 SNIRGLLLNVHEHLAVVGVKPNIV*HKTNVAAGLPDNLLVVNIGLGGDLTKDHDHVGLGA 318
SNIR LLLNV + LA VG+K NI+ ++N AA + ++LL+V +GLG +KDHDHV LGA
Sbjct: 435 SNIRTLLLNVDQDLAFVGIKTNIIRDESNGAACVTNDLLIVYVGLGCYFSKDHDHVSLGA 256
Query: 317 GLTSNLAVRVLSKASVKNGI*DLVTELVRVSLVHRLR 207
LT LAVRVL K SV++ I DLV +LV V+LVH R
Sbjct: 255 CLTGTLAVRVLLKTSVEHCIRDLVAQLVGVTLVH*FR 145
>gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 205
Score = 108 bits (230), Expect = 2e-024
Identities = 48/66 (72%), Positives = 50/66 (75%)
Frame = +3 / +3
Query: 933 TGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYVARQAAKSIVASGLARRAIVQVSYA 1112
TGRKIIIDTY KDPTKVDRSGAY+ RQAAKSIVA+GLARR IVQVSYA
Sbjct: 3 TGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYA 182
Query: 1113 IGVPEP 1130
IGVP P
Sbjct: 183 IGVPXP 200
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,414,182
Number of Sequences: 7669
Number of extensions: 200293
Number of successful extensions: 2598
Number of sequences better than 1.0e-020: 7
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 459210528
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)