TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419471.2.3
(1052 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY560003.1| Medicago sativa S-adenosylmethionine synthas... 501 e-143
gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular tr... 248 1e-066
gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted... 106 4e-024
>gb|AY560003.1| Medicago sativa S-adenosylmethionine synthase mRNA, complete cds
Length = 1173
Score = 501 bits (1089), Expect = e-143
Identities = 209/242 (86%), Positives = 218/242 (90%)
Frame = -3 / +1
Query: 1050 GALLTEVRKNGTCPWLRPDGKTQVTVEYRNEGGAMVPIRVHTVLISTQHDETVTNDEIAA 871
GA LTEVRKNGTCPWLRPDGKTQVTVEY N+ GAMVP+RVHTVLISTQHDETVTNDEIAA
Sbjct: 445 GARLTEVRKNGTCPWLRPDGKTQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAA 624
Query: 870 DLKEHVIKPVIPEQYLDEKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXX 691
DLKEHVIKPVIPE+YLD KTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTY
Sbjct: 625 DLKEHVIKPVIPEKYLDSKTIFHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGG 804
Query: 690 XXXGKDPTKVDRSGAYVARQAAKSIVAAGLARRAIVQVSYAIGVPEPLSVFVDTYGTGAI 511
GKDPTKVDRSGAY+ RQAAKSIVA+GLARR IVQVSYAIGVPEPLSVFVDTYGTG I
Sbjct: 805 AFSGKDPTKVDRSGAYIVRQAAKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKI 984
Query: 510 PDKEILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKA 331
PDKEIL IVK+NFDFRPGMI INLDL +GGNGR+LKTAAYGHFGR+D DFTWEVVKPLK
Sbjct: 985 PDKEILNIVKQNFDFRPGMISINLDLLRGGNGRFLKTAAYGHFGREDADFTWEVVKPLKW 1164
Query: 330 EK 325
EK
Sbjct: 1165 EK 1170
Score = 193 bits (417), Expect(3) = 1e-079
Identities = 87/110 (79%), Positives = 93/110 (84%)
Frame = +2 / -2
Query: 722 VSMMIFRPVRPASP*GPPMTKRPDGLRWKIVFSSRYCSGMTGLMTCSFRSAAISSLVTVS 901
VSMMIF PVRPASP*GPPMTKRP+G + K+V SRY SG+TG +TCS RSAAISSLVTVS
Sbjct: 773 VSMMIFLPVRPASP*GPPMTKRPEGFK*KMVLESRYFSGITGFITCSLRSAAISSLVTVS 594
Query: 902 SCWVEMRTVCTRMGTMAPPSLRYSTVTWVFPSGLSQGQVPFLRTSVRRAP 1051
SC VEM TV TR GTMAP SL YSTVT V PSGL+QG VPFLRTSVRRAP
Sbjct: 593 SC*VEMSTVWTRTGTMAPLSL*YSTVT*VLPSGLNQGHVPFLRTSVRRAP 444
Score = 108 bits (230), Expect(3) = 5e-027
Identities = 48/115 (41%), Positives = 58/115 (50%)
Frame = -2 / +3
Query: 1051 WCSSHGGSQEWNLPLAQARWEDPGDSGVPQRGWRHGSHPCAHSPHLYPARRDSHQRRDCC 872
WCSSH GSQEW +PL + W D V Q W HG C H HLY D +Q +CC
Sbjct: 444 WCSSH*GSQEWYMPLVET*W*DSSHC*VLQ*QWCHGPCSCPHCAHLYST**DCYQ**NCC 623
Query: 871 *PEGARHQASHPRAVPRREDNLPPQPVWPLXXXXXXXXXRPHWPEDHHRHLRWLG 707
* + A ++A VP +D+ +P WP HW EDHH HL WLG
Sbjct: 624 *SQRACYKACDS*EVP*FQDHFSLEPFWPFRHWRSSR*CWSHW*EDHH*HLWWLG 788
Score = 90.0 bits (190), Expect(3) = 1e-079
Identities = 38/43 (88%), Positives = 40/43 (93%)
Frame = +2 / -2
Query: 452 IMPGLKSKFSFTIFRISLSGIAPVPYVSTNTDRGSGTPMA*ET 580
IMPGLKSKF FTI RISLSGI PVPYVSTNTD+GSGTP+A*ET
Sbjct: 1043 IMPGLKSKFCFTILRISLSGIFPVPYVSTNTDKGSGTPIA*ET 915
Score = 64.3 bits (134), Expect(3) = 2e-032
Identities = 27/60 (45%), Positives = 40/60 (66%)
Frame = +1 / -1
Query: 871 SSNLVVGDCLVVLGRDEDCVHTDGNHGATLVAVLHCHLGLPIGPEPGAGSILANLREKST 1050
SSN ++G+ L++L RDE V T+ +HG ++ +L+ L L I +P A +ILANL E ST
Sbjct: 624 SSNFIIGNSLIMLSRDEHSVDTNRDHGTIVIVILNSDLSLTIRSQPRACTILANLSETST 445
Score = 57.9 bits (120), Expect(3) = 2e-032
Identities = 24/56 (42%), Positives = 35/56 (62%)
Frame = +1 / -1
Query: 628 SLPRDVGPAAVHLGRVLAGEGAPAVGSPATVGVDDDLPASEAGVAVRSADDEAARR 795
SLP +V V+L V + E + +P T+ V+DDLP SE + VR+++DE ARR
Sbjct: 867 SLPHNVSSTPVNLSWVFS*ESTTTMCTPTTISVNDDLPTSETSITVRTSNDETARR 700
Score = 52.8 bits (109), Expect(3) = 1e-079
Identities = 23/26 (88%), Positives = 23/26 (88%)
Frame = +2 / -2
Query: 326 FSALRGFTTSQVKSGSSLPKCP*AAV 403
FS LRGFTTS VKS SSLPKCP*AAV
Sbjct: 1169 FSHLRGFTTSHVKSASSLPKCP*AAV 1092
Score = 49.6 bits (102), Expect(3) = 2e-032
Identities = 19/47 (40%), Positives = 32/47 (68%)
Frame = +1 / -1
Query: 403 LEVAPVAAFLEVEVDDDHAGPEVEVLLHDLQDLLVGDRAGAVRVHEH 543
L+ +A E++VD DHA PE++VLLH+++D L+ D + V++H
Sbjct: 1092 LQEPAIATPQEIKVDRDHARPEIKVLLHNIEDFLIRDLPCTICVNKH 952
Score = 25.8 bits (50), Expect(3) = 5e-027
Identities = 12/23 (52%), Positives = 13/23 (56%)
Frame = -2 / +3
Query: 394 LRALWEGRPRLHLGGGEAPQGGE 326
L W GR LH+G GEA GE
Sbjct: 1101 LWTFW*GRC*LHMGSGEAS*MGE 1169
Score = 25.4 bits (49), Expect(3) = 5e-027
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = -2 / +3
Query: 532 HVRHRRDPRQGDPEDREGELRLQA 461
H+ +R DP G+P+ E EL QA
Sbjct: 963 HIWYREDP**GNPQYCEAEL*FQA 1034
>gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 505
Score = 248 bits (535), Expect = 1e-066
Identities = 103/118 (87%), Positives = 108/118 (91%)
Frame = -3 / +3
Query: 678 KDPTKVDRSGAYVARQAAKSIVAAGLARRAIVQVSYAIGVPEPLSVFVDTYGTGAIPDKE 499
KDPTKVDRSGAY+ RQAAKSIVA GLARR IVQVSYAIGVPEPLSVFVD+YGTG IPDKE
Sbjct: 24 KDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKE 203
Query: 498 ILKIVKENFDFRPGMIIINLDLKKGGNGRYLKTAAYGHFGRDDPDFTWEVVKPLKAEK 325
IL IVKE+FDFRPGMI INLDLK+GGN R+LKTAAYGHFGRDD DFTWEVVKPLK K
Sbjct: 204 ILNIVKESFDFRPGMISINLDLKRGGNNRFLKTAAYGHFGRDDADFTWEVVKPLKGGK 377
Score = 76.2 bits (160), Expect(2) = 7e-022
Identities = 34/43 (79%), Positives = 37/43 (86%)
Frame = +2 / -1
Query: 452 IMPGLKSKFSFTIFRISLSGIAPVPYVSTNTDRGSGTPMA*ET 580
I+PGLKSK SFT+ RISLSGI PVP STNTD GSGTP+A*ET
Sbjct: 250 IIPGLKSKDSFTMLRISLSGIFPVP*ESTNTDNGSGTPIA*ET 122
Score = 42.3 bits (86), Expect(2) = 7e-022
Identities = 20/26 (76%), Positives = 20/26 (76%)
Frame = +2 / -1
Query: 326 FSALRGFTTSQVKSGSSLPKCP*AAV 403
F L GFTTS VKS SSLPK P*AAV
Sbjct: 376 FPPLSGFTTSHVKSASSLPK*P*AAV 299
>gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 205
Score = 106 bits (227), Expect = 4e-024
Identities = 49/66 (74%), Positives = 50/66 (75%)
Frame = -3 / +3
Query: 747 TGRKIIIDTYXXXXXXXXXXXXGKDPTKVDRSGAYVARQAAKSIVAAGLARRAIVQVSYA 568
TGRKIIIDTY GKDPTKVDRSGAY+ RQAAKSIVA GLARR IVQVSYA
Sbjct: 3 TGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYA 182
Query: 567 IGVPEP 550
IGVP P
Sbjct: 183 IGVPXP 200
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,432,776
Number of Sequences: 7669
Number of extensions: 116956
Number of successful extensions: 4582
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 282071907
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)