TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2419155.2.3
(1354 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|X70707.1|MSCDC2 M.sativa mRNA for CDC2 kinase 607 e-174
gb|M58365.1|ALFPKSTA Medicago sativa CDC2MS serine/threonin... 588 e-169
emb|X97315.1|MSCDC2MSD M.sativa mRNA for cdc2 kinase homolo... 239 e-109
emb|X97317.1|MSCDC2MSF M.sativa mRNA for cdc2 kinase homolo... 228 e-105
gb|AF302013.1| Medicago sativa subsp. x varia CDK-activatin... 141 1e-080
emb|X97314.1|MSCDC2MSC M.sativa mRNA for cdc2 kinase homolo... 116 5e-074
gb|L07042.1|ALFERKMAP Medicago sativa MAP kinase MsERK1 mRN... 95 1e-054
emb|X66469.1|MSMSK7 M.sativa mRNA msk7 for map kinase 95 1e-054
emb|X82270.1|MSMMK4 M.sativa MMK4 mRNA for protein kinase 96 2e-053
emb|X82268.1|MSMMK2 M.sativa MMK2 mRNA for protein kinase 88 6e-052
emb|AJ224336.1|MSAJ4336 Medicago sativa mRNA for MAP kinase... 94 1e-051
emb|X97316.1|MSCDC2MSE M.sativa mRNA for cdc2 kinase homolo... 78 5e-051
emb|X68411.1|AMMSK1A A.medicago MSK-1 mRNA for protein kinase 91 8e-032
emb|X68409.1|AMMSK3A A.medicago MSK-3 mRNA for protein kinase 90 1e-031
emb|X68410.1|AMMSK2A A.medicago MSK-2 mRNA for protein kinase 86 3e-031
emb|AX774637.1| Sequence 2 from Patent WO03035856 91 3e-030
gb|AF432225.1| Medicago sativa GSK-3-like protein MsK4 mRNA... 91 3e-030
emb|AJ295939.1|MSA295939 Medicago sativa mRNA for wound-ind... 83 1e-029
gb|CO514154.1|CO514154 s13dSG75H0800064_156970 Glandular tr... 66 7e-027
gb|CO512589.1|CO512589 s13dSG13A0100001_121104 Glandular tr... 81 2e-026
gb|CO515142.1|CO515142 s13dSG40H0100016_399149 Glandular tr... 65 2e-022
>emb|X70707.1|MSCDC2 M.sativa mRNA for CDC2 kinase
Length = 1308
Score = 607 bits (1319), Expect = e-174
Identities = 249/293 (84%), Positives = 271/293 (92%)
Frame = +2 / +1
Query: 242 MEQYEKVEKIGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISLLKEMNH 421
MEQYEKVEKIGEGTYGVVYKA D+ATNETIALKKIRLEQEDEGVPSTAIREISLLKEM H
Sbjct: 70 MEQYEKVEKIGEGTYGVVYKARDRATNETIALKKIRLEQEDEGVPSTAIREISLLKEMQH 249
Query: 422 GNIVRLHDVVHSEKRIYLVFEYLDLDLKKFMDSCPEFAKNPTLIKSYLYQILRGVAYCHS 601
NIVRL DVVHSEKR+YLVFEYLDLDLKKFMDS PEFAK+ IK +LYQIL G+AYCHS
Sbjct: 250 RNIVRLQDVVHSEKRLYLVFEYLDLDLKKFMDSSPEFAKDQRQIKMFLYQILCGIAYCHS 429
Query: 602 HRVLHRDLKPQNLLIDRRTNALKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGARQ 781
HRVLHRDLKPQNLLIDR +NA+KLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLG+R
Sbjct: 430 HRVLHRDLKPQNLLIDRSSNAVKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSRH 609
Query: 782 YSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILGTPNEQSWPGVSCLPDFKTA 961
YSTPVDVWSVGCIFAEM+NQ+PLFPGDSEIDELFKIFRI GTPNE++WPGV+ LPDFK+A
Sbjct: 610 YSTPVDVWSVGCIFAEMINQRPLFPGDSEIDELFKIFRITGTPNEETWPGVTSLPDFKSA 789
Query: 962 FPRWQAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLEVV 1120
FP+W A+DLAT VPNL+PAGLDLLS R +P++RITAR ALEHEYFKD++ V
Sbjct: 790 FPKWPAKDLATQVPNLEPAGLDLLSSTCRLDPTRRITARGALEHEYFKDIKFV 948
Score = 380 bits (824), Expect = e-106
Identities = 190/295 (64%), Positives = 206/295 (69%)
Frame = -2 / -2
Query: 1125 HCTTSRSLKYSCSSACLAVILLLGSYRSILERRSNPAGSRFGTTVARS*ACHLGKAVLKS 946
H T SLKYSCS A LAVILL+GS R +L+RRS PAGSRFGT VARS A HLGKA LKS
Sbjct: 953 HGTNLMSLKYSCSRAPLAVILLVGSKRQVLDRRSRPAGSRFGT*VARSLAGHLGKADLKS 774
Query: 945 GKQLTPGQLCSFGVPXXXXXXXXXXXXXXXXXSGFWFTISAKIQPTDHTSTGVEYCLAPS 766
G ++TPG + SFGVP SG WF ISA + PTD TSTGVE PS
Sbjct: 773 GNEVTPGHVSSFGVPVILNILNNSSISESPGKSGRWFIISANMHPTDQTSTGVE**RDPS 594
Query: 765 RISGALYHNVTTS*VNVRTGIPNALAKPKSASFSALVRRSINKF*GFKSR*RTL*EWQYA 586
ISGALY +VTTS VNV TGIPNALA PKSASF+AL RSI++F GFKS *RTL*E Q A
Sbjct: 593 NISGALYQSVTTSCVNVLTGIPNALANPKSASFTALELRSISRFCGFKSL*RTL*E*Q*A 414
Query: 585 TPRSIW*RYDLIKVGFLANSGQESMNFLRSRSRYSKTRYMRFSLWTTSCNLTMLPWFISL 406
P+ I *R I ANSG ES+NF RS+SRYSKTRY RFSL TTSCNLTM IS
Sbjct: 413 MPQRI**RNIFICR*SFANSGDESINFFRSKSRYSKTRYNRFSLCTTSCNLTMFLCCISF 234
Query: 405 RREISRMAVDGTPSSSCSRRIFXXXXXXXXXXXXXLYTTPYVPSPIFSTFSYCSI 241
+REISR+AV GTPSSSCSRRIF L TTP VPSPIFSTFSYCSI
Sbjct: 233 KREISRIAVLGTPSSSCSRRIFFKAIVSLVARSRAL*TTP*VPSPIFSTFSYCSI 69
Score = 243 bits (525), Expect = 4e-065
Identities = 113/243 (46%), Positives = 158/243 (65%)
Frame = -1 / -1
Query: 1108 VLEVLMLKCLPRCDSFAWLVSKHFREKVQPSRVKIWDYCCQVLSLPPGESCLEVRQTTDS 929
V EV MLK P +S + + K++ S ++IWD CQVLS P G+ ++RQ + S
Sbjct: 936 VFEVFMLKSSPGSNSSGGIQATSTG*KIKTSWLQIWDLSCQVLSWPLGKG*FKIRQ*SHS 757
Query: 928 WPTLLIRCT*YPEYLKKFVDLRIARE*WLLVHHFRKDTAHRPHINWCGILPRSKQNFWSS 749
P LIRCT EY K+F++LR+ E W LV+H K +H P IN I+ R KQ FWSS
Sbjct: 756 RPCFLIRCTRDSEYFKQFINLRVPWEKWPLVYHLCKYASH*PDINRGRIMTRPKQYFWSS 577
Query: 748 VP*CHYLMSKCTDRNSKCPG*TKVCKLQCISAPIYQ*VLRFQISVKNSMRMAVRNTTEYL 569
VP CH+LM KC DRNSKCPG +K+CKL I A I Q +L FQ+S+KNSMRM V N TE L
Sbjct: 576 VPECHHLMCKCPDRNSKCPGQSKICKLYGIRAAINQQILWFQVSMKNSMRMTVSNATENL 397
Query: 568 VEV*LDQSGILSKLRAGVHELLEVQIQVLEDKVYALLAVDNIM*SDDVAVVHLLEERDLA 389
++ L I K +H+LL +Q++VL++++ +LL V +I+ ++V V+H L+ER+L+
Sbjct: 396 IKEHLYLPLIFCKFWR*IHKLL*IQVKVLKNQIQSLLTVHHILQPNNVPVLHFLQERNLS 217
Query: 388 DGG 380
+ G
Sbjct: 216 NSG 208
Score = 192 bits (414), Expect = 8e-050
Identities = 98/237 (41%), Positives = 137/237 (57%)
Frame = +1 / +3
Query: 394 DLSPQGDEPRQHRQIT*CCPQREAHIPCLRVPGSGPQEVHGLLPGVC*ESHFDQVIPLPD 573
D S +G+ ++H + C Q EA + VP G +EV+G + +C S ++ +PL +
Sbjct: 222 DFSLEGNAAQEHC*VAGCGAQ*EAIVSGF*VP*LGSKEVYGFISRICKRSTANKDVPLSN 401
Query: 574 TPWCCVLPFS*SSSPRFETSELIDRSAH*CTEACRLWFSQGIWNSCPYIYS*GSDIMVQS 753
+ W C+L FS*SSS R ET+E D S *C +ACR W QGIWNSC IY+*G D +VQS
Sbjct: 402 SLWHCLLSFS*SSS*RLETTESAD*SQL*CRKACRFWIGQGIWNSCQDIYT*GGDTLVQS 581
Query: 754 SRNSAWSEAVFHTS*CVVCGLYLCGNGEPKATIPWRF*DRRTF*DIQDTRYTE*AELARS 933
SRN AW ++F+ *C+V G+++C + +P AT P *D *+IQ+ YTE* +A S
Sbjct: 582 SRNIAWVSSLFYPG*CLVSGMHICRDDKPTATFPRGL*D**IV*NIQNHGYTE*GNMAWS 761
Query: 934 QLFA*LQDSFPQVAXXXXXXXXXXXXXCWVGPSL*NASIRAKQKNHSEAST*A*VLQ 1104
A* S QVA W S+ S+ + QKN+ + S+*A*+LQ
Sbjct: 762 DFIA*F*ISLSQVAS*GPGNSSPKSGASWS*SSIQYLSLGSHQKNYCQGSS*A*ILQ 932
Score = 151 bits (324), Expect = 2e-037
Identities = 82/150 (54%), Positives = 94/150 (62%)
Frame = +3 / +2
Query: 570 RYSVVLRTAILIEFFTEI*NLRTY**IGALMH*SLQTLV*PGHLEFLSVHLLMR**HYGT 749
++SV L T ILIEFF E *N R * I ALM *SLQ L PGHLEFLS HL MR *H GT
Sbjct: 398 KFSVALLTVILIEFFIET*NHRIC*LIAALMP*SLQILDWPGHLEFLSGHLHMRW*HSGT 577
Query: 750 ELQKFCLERGSIPHQLMCGLWAVSLRKW*TKSHYSLAILRSTNFLRYSGY*VHRMSRVGQ 929
ELQK+CL I +LM G W L++ *T H+S LR N L+YS VHRM + G
Sbjct: 578 ELQKYCLGLVIILPRLMSGQWDAYLQR**TNGHFSQGTLRLMNCLKYSESRVHRMRKHGL 757
Query: 930 ESVVCLTSRQLSPGGKLRTWQQ*SQILTLL 1019
E + CL Q P G+LRTWQ SQI + L
Sbjct: 758 E*LHCLILNQPFPSGQLRTWQLKSQIWSQL 847
Score = 138 bits (296), Expect = 1e-033
Identities = 84/178 (47%), Positives = 97/178 (54%)
Frame = -3 / -3
Query: 977 LATWGKLS*SQANN*LLANSAHSVYLVS*IS*KVRRSQNRQGIVAFGSPFPQRYSPQTTH 798
LATW +L *+QA L A HSVY *I * + +SQ+ G VA G Q P T H
Sbjct: 805 LATWERLI*NQAMKSLQAMFPHSVYP*F*IF*TIHQSQSPLGKVAVGLSSLQICIPLTRH 626
Query: 797 QLVWNTASLQAEFLELCTIMSLPHE*MYGQEFQMPWLNQSLQASVH*CADLSISSEVSNL 618
Q N QA FLELCT +S PH *M QEFQMPW Q+LQA H* D S S VS+L
Sbjct: 625 QPG*NNDETQAIFLELCTRVSPPHV*MS*QEFQMPWPIQNLQALRH*SCDQSADSVVSSL 446
Query: 617 GEELYENGSTQHHGVSGRGMT*SKWDS*QTPGRSP*TS*GPDPGTRRQGICASRCGQH 444
EELYEN S Q H +G + D Q +P*TS P GT++ AS C H
Sbjct: 445 YEELYENDSKQCHREFDKGTSLFAVDLLQILEMNP*TSLDPSQGTQKPDTIASHCAPH 272
>gb|M58365.1|ALFPKSTA Medicago sativa CDC2MS serine/threonine protein kinase (CDC2MS)
mRNA, partial cds
Length = 998
Score = 588 bits (1279), Expect = e-169
Identities = 240/289 (83%), Positives = 264/289 (91%)
Frame = +2 / +2
Query: 254 EKVEKIGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISLLKEMNHGNIV 433
E VEKIGEGTYGVVYKA D+ TNETIALKKIRLEQEDEGVPSTAIREISLLKEM H NIV
Sbjct: 5 ENVEKIGEGTYGVVYKARDRVTNETIALKKIRLEQEDEGVPSTAIREISLLKEMQHRNIV 184
Query: 434 RLHDVVHSEKRIYLVFEYLDLDLKKFMDSCPEFAKNPTLIKSYLYQILRGVAYCHSHRVL 613
RL DVVHS+KR+YLVFEYLDLDLKK MDS PEF K+P +K +LYQ+L G+AYCHSHRVL
Sbjct: 185 RLQDVVHSDKRLYLVFEYLDLDLKKHMDSSPEFIKDPRQVKMFLYQMLCGIAYCHSHRVL 364
Query: 614 HRDLKPQNLLIDRRTNALKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGARQYSTP 793
HRDLKPQNLLIDRRTN+LKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLG+R YSTP
Sbjct: 365 HRDLKPQNLLIDRRTNSLKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGSRHYSTP 544
Query: 794 VDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILGTPNEQSWPGVSCLPDFKTAFPRW 973
VDVWSVGCIFAEM N++PL PGDSEIDELFKIFRILGTPNE +WPGV+ LPDFK+ FPRW
Sbjct: 545 VDVWSVGCIFAEMANRRPLSPGDSEIDELFKIFRILGTPNEDTWPGVTSLPDFKSTFPRW 724
Query: 974 QAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLEVV 1120
++DLATVVPNL+PAGLDLL+ ML +P+KRITAR A+EHEYFKD++ V
Sbjct: 725 PSKDLATVVPNLEPAGLDLLNSMLCLDPTKRITARSAVEHEYFKDIKFV 871
Score = 363 bits (786), Expect = e-101
Identities = 184/289 (63%), Positives = 195/289 (67%)
Frame = -2 / -3
Query: 1119 TTSRSLKYSCSSACLAVILLLGSYRSILERRSNPAGSRFGTTVARS*ACHLGKAVLKSGK 940
T SLKYSCS+A LAVILL+GS +S+L RRS PAGSRFGTTVARS HLG LKSG
Sbjct: 870 TNLMSLKYSCSTALLAVILLVGSKQSMLLRRSRPAGSRFGTTVARSLDGHLGNVDLKSGN 691
Query: 939 QLTPGQLCSFGVPXXXXXXXXXXXXXXXXXSGFWFTISAKIQPTDHTSTGVEYCLAPSRI 760
++TPG + SFGVP G F ISA I PTD TSTGVE PS I
Sbjct: 690 EVTPGHVSSFGVPKILNILNNSSISESPGDRGRRFAISANIHPTDQTSTGVE**RDPSNI 511
Query: 759 SGALYHNVTTS*VNVRTGIPNALAKPKSASFSALVRRSINKF*GFKSR*RTL*EWQYATP 580
SGA YH+VTTS VNV TGIPNALA PKSAS S LVRRSINKF GFKSR*RTL E Q A P
Sbjct: 510 SGARYHSVTTSCVNVLTGIPNALANPKSASLSELVRRSINKFCGFKSR*RTLCE*Q*AIP 331
Query: 579 RSIW*RYDLIKVGFLANSGQESMNFLRSRSRYSKTRYMRFSLWTTSCNLTMLPWFISLRR 400
+SI *R G NSG ES+ F RS+SRYSKTRY R SL TTSCNLTM ISL
Sbjct: 330 QSI**RNIFTCRGSFINSGDESICFFRSKSRYSKTRYNRLSLCTTSCNLTMFLCCISLSS 151
Query: 399 EISRMAVDGTPSSSCSRRIFXXXXXXXXXXXXXLYTTPYVPSPIFSTFS 253
EISRMAV GTPSSSCS RIF L TTPYVPSPIFSTFS
Sbjct: 150 EISRMAVLGTPSSSCSSRIFFNAMVSFVTRSRAL*TTPYVPSPIFSTFS 4
Score = 236 bits (510), Expect = 4e-063
Identities = 107/246 (43%), Positives = 161/246 (65%)
Frame = -1 / -2
Query: 1120 YHFKVLEVLMLKCLPRCDSFAWLVSKHFREKVQPSRVKIWDYCCQVLSLPPGESCLEVRQ 941
Y V E+ ML P +SF + +KH +K++ S +KIW++ C VL PPG+ ++RQ
Sbjct: 871 YKLNVFEIFMLHSAPGSNSFGGIQAKHAIKKIKTSWLKIWNHGC*VLRWPPGKC*FKIRQ 692
Query: 940 TTDSWPTLLIRCT*YPEYLKKFVDLRIARE*WLLVHHFRKDTAHRPHINWCGILPRSKQN 761
+ SWP + I CT EY K ++L I R * + H K T+H P+INW I+ RSKQ
Sbjct: 691 *SYSWPCIFIWCTQDSEYFK*LINLGIPRG*RSPICHLCKYTSH*PNINWGRIMTRSKQY 512
Query: 760 FWSSVP*CHYLMSKCTDRNSKCPG*TKVCKLQCISAPIYQ*VLRFQISVKNSMRMAVRNT 581
F SSVP C+YLM KC+DRN+KCPG +K+ KL+ IS IYQ +L FQ+S+KNS+ M V N+
Sbjct: 511 FRSSVPQCNYLMCKCSDRNTKCPGQSKIGKLK*ISTAIYQQILWFQVSMKNSV*MTVSNS 332
Query: 580 TEYLVEV*LDQSGILSKLRAGVHELLEVQIQVLEDKVYALLAVDNIM*SDDVAVVHLLEE 401
TE+L++ S I KLR +H LL++ +Q+L++++ +L+ V I+ +DV ++H ++
Sbjct: 331 TEHLIKEHFYLSRIFYKLRR*IHMLLQI*VQILKNQIQSLVTVHYILQPNDVPMLHFFKQ 152
Query: 400 RDLADG 383
R+L +G
Sbjct: 151 RNLTNG 134
Score = 161 bits (347), Expect = 1e-040
Identities = 78/184 (42%), Positives = 109/184 (59%)
Frame = +1 / +1
Query: 424 QHRQIT*CCPQREAHIPCLRVPGSGPQEVHGLLPGVC*ESHFDQVIPLPDTPWCCVLPFS 603
+HR + C Q +A + V G +E +G + V S + +PL + W C+L F+
Sbjct: 175 EHR*VAGCSAQ*QAIVSGF*VSGLRSEEAYGFIS*VYKRSATSKNVPLSNALWNCLLSFT 354
Query: 604 *SSSPRFETSELIDRSAH*CTEACRLWFSQGIWNSCPYIYS*GSDIMVQSSRNSAWSEAV 783
SSS R ET+E +DRS +* T ACR W QGIW SC IY+*GS +V SS N AW ++
Sbjct: 355 QSSSSRLETTEFVDRSPY*FT*ACRFWIGQGIWYSCQNIYT*GSYTVVPSSGNIAWISSL 534
Query: 784 FHTS*CVVCGLYLCGNGEPKATIPWRF*DRRTF*DIQDTRYTE*AELARSQLFA*LQDSF 963
F+ S*C+V G+Y+C +G+ +IPW F D *+IQ+ YT+* +ARS A* +
Sbjct: 535 FYPS*CLVSGMYICRDGKSATSIPWGFRD**VI*NIQNLGYTK*RYMARSNFIA*F*INI 714
Query: 964 PQVA 975
QVA
Sbjct: 715 SQVA 726
Score = 152 bits (326), Expect = 1e-037
Identities = 89/196 (45%), Positives = 109/196 (55%)
Frame = +3 / +3
Query: 576 SVVLRTAILIEFFTEI*NLRTY**IGALMH*SLQTLV*PGHLEFLSVHLLMR**HYGTEL 755
SV L T I EFF E *N R **I L+H SL L PGHL FLS HL MR* H GTEL
Sbjct: 327 SVELLTVIHTEFFIET*NHRIC**IAVLIHLSLPILDWPGHLVFLSEHLHMR*LHCGTEL 506
Query: 756 QKFCLERGSIPHQLMCGLWAVSLRKW*TKSHYSLAILRSTNFLRYSGY*VHRMSRVGQES 935
+K+CL+ I QLM G W V L++W Y L I R ++L+YS VH+M GQE
Sbjct: 507 RKYCLDLVIILPQLMFGQWDVYLQRWQIGDLYPLGIPRLMSYLKYSESWVHQMKIHGQE* 686
Query: 936 VVCLTSRQLSPGGKLRTWQQ*SQILTLLGWTFSLKCFDTSQAKESQRGKHLSMSTSRTLK 1115
+ CL Q PGG LRT Q QIL+ L F + CF KE G SM+ S+TL
Sbjct: 687 LHCLILNQHFPGGHLRT*QPWFQILSQLVLIFLIACFAWIPPKELLPGALWSMNISKTLS 866
Query: 1116 WYSDLLNVLDVALTFV 1163
Y + + + + F+
Sbjct: 867 LYPNSIIYILMVKVFI 914
Score = 113 bits (242), Expect = 4e-026
Identities = 67/139 (48%), Positives = 80/139 (57%)
Frame = -3 / -1
Query: 977 LATWGKLS*SQANN*LLANSAHSVYLVS*IS*KVRRSQNRQGIVAFGSPFPQRYSPQTTH 798
+ATW L *+QA LLA H VY *I * +S+N QGI P Q Y P T H
Sbjct: 728 MATWEMLI*NQAMKLLLAMYLHLVYPRF*IF*ITHQSRNPQGIEVADLPSLQIYIPLTKH 549
Query: 797 QLVWNTASLQAEFLELCTIMSLPHE*MYGQEFQMPWLNQSLQASVH*CADLSISSEVSNL 618
QL N +QA F EL T + LPH *M+ QE+QMPW Q+ QA V+* DLS +S VS+L
Sbjct: 548 QLG*NNDEIQAIFPELGTTV*LPHV*MF*QEYQMPWPIQNRQA*VN*YGDLSTNSVVSSL 369
Query: 617 GEELYENGSTQHHGVSGRG 561
EEL N S Q H +G
Sbjct: 368 DEELCVNDSKQFHRAFDKG 312
>emb|X97315.1|MSCDC2MSD M.sativa mRNA for cdc2 kinase homologue, cdc2MsD
Length = 1274
Score = 239 bits (516), Expect(4) = e-109
Identities = 89/136 (65%), Positives = 114/136 (83%)
Frame = +2 / +1
Query: 545 TLIKSYLYQILRGVAYCHSHRVLHRDLKPQNLLIDRRTNALKLADFGLARAFGIPVRTFT 724
TL++S+L+Q+ +GVA+CHSH VLHRDLKPQNLL+D+ LK+AD GL RAF +P++++T
Sbjct: 388 TLVQSFLFQLCKGVAHCHSHGVLHRDLKPQNLLLDQAKGILKIADLGLGRAFTVPLKSYT 567
Query: 725 HEVVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILG 904
HE+VTLWYRAPE+LLG+ YST VD+WSVGCIFAEMV ++ LFPGDSE +L IF++LG
Sbjct: 568 HEIVTLWYRAPEVLLGSSTYSTGVDIWSVGCIFAEMVRRQALFPGDSEFQQLLNIFKLLG 747
Query: 905 TPNEQSWPGVSCLPDF 952
TP EQ WPGVS L D+
Sbjct: 748 TPTEQQWPGVSSLRDW 795
Score = 93.2 bits (197), Expect(4) = e-109
Identities = 36/59 (61%), Positives = 50/59 (84%)
Frame = +2 / +1
Query: 242 MEQYEKVEKIGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISLLKEMN 418
ME+YEK+EK+GEGTYG VYKA + +T + +ALKK RLE ++EGVP TA+RE+SLL+ ++
Sbjct: 34 MEKYEKLEKVGEGTYGKVYKAKELSTGQIVALKKTRLEMDEEGVPPTALREVSLLQMLS 210
Score = 81.7 bits (172), Expect(5) = 8e-048
Identities = 48/85 (56%), Positives = 50/85 (58%)
Frame = -2 / -1
Query: 834 TISAKIQPTDHTSTGVEYCLAPSRISGALYHNVTTS*VNVRTGIPNALAKPKSASFSALV 655
TISAKI PTD ST VE PS+ SGALY VT S*V + G AL PKSA F
Sbjct: 677 TISAKIHPTDQISTPVE*VEEPSKTSGALYQRVTIS*V*LFNGTVKALPSPKSAIFKIPF 498
Query: 654 RRSINKF*GFKSR*RTL*EWQYATP 580
S NKF GFKSR T * Q ATP
Sbjct: 497 A*SSNKFCGFKSRCNTP*L*Q*ATP 423
Score = 79.0 bits (166), Expect(4) = e-109
Identities = 29/54 (53%), Positives = 42/54 (77%)
Frame = +2 / +1
Query: 962 FPRWQAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLEVVQ 1123
+PRW+ Q+LA VP+L P G+DLL+KML+Y P++RI+A+ AL+H YF L+ Q
Sbjct: 802 YPRWEPQNLARAVPSLSPDGVDLLTKMLKYNPAERISAKAALDHPYFDSLDKSQ 963
Score = 78.0 bits (164), Expect(2) = 2e-021
Identities = 44/115 (38%), Positives = 66/115 (57%)
Frame = -1 / -3
Query: 892 EYLKKFVDLRIARE*WLLVHHFRKDTAHRPHINWCGILPRSKQNFWSSVP*CHYLMSKCT 713
EY++K ++ RI + L HHF K+T++RP+IN + R+KQN SS+P LMS
Sbjct: 735 EYVQKLLEFRIPWKQSLSSHHFSKNTSNRPNINTSRVSRRTKQNLRSSIPKSDNLMSITF 556
Query: 712 DRNSKCPG*TKVCKLQCISAPIYQ*VLRFQISVKNSMRMAVRNTTEYLVEV*LDQ 548
R+SK + LQ I Q +LRFQI++ ++M M + NT L + L+Q
Sbjct: 555 QRHSKSSPKP*ISNLQNPFRLIQQQILRFQITM*HTMTMTMSNTFTQLKQKTLNQ 391
Score = 67.0 bits (140), Expect(5) = 8e-048
Identities = 32/56 (57%), Positives = 35/56 (62%)
Frame = -2 / -1
Query: 405 RREISRMAVDGTPSSSCSRRIFXXXXXXXXXXXXXLYTTPYVPSPIFSTFSYCSIA 238
RRE SR AV GTPSSS SRR+F LYT P VPSP FS+FSY SI+
Sbjct: 197 RRETSRSAVGGTPSSSISRRVFLSATIWPVESSFALYTFP*VPSPTFSSFSYFSIS 30
Score = 52.8 bits (109), Expect(5) = 8e-048
Identities = 27/50 (54%), Positives = 30/50 (60%)
Frame = -2 / -1
Query: 1113 SRSLKYSCSSACLAVILLLGSYRSILERRSNPAGSRFGTTVARS*ACHLG 964
SR KY SSA LA IL G Y SI RRS P+G GT +A+ * HLG
Sbjct: 953 SRLSKYG*SSAALADILSAGLYLSIFVRRSTPSGDNEGTALAKF*GSHLG 804
Score = 39.6 bits (80), Expect(4) = e-109
Identities = 15/22 (68%), Positives = 19/22 (86%)
Frame = +2 / +1
Query: 455 SEKRIYLVFEYLDLDLKKFMDS 520
S+ +YLVFEYLD DLKKF+D+
Sbjct: 286 SKPLLYLVFEYLDTDLKKFIDT 351
Score = 39.1 bits (79), Expect(2) = 2e-021
Identities = 20/48 (41%), Positives = 28/48 (58%)
Frame = -1 / -3
Query: 1111 KVLEVLMLKCLPRCDSFAWLVSKHFREKVQPSRVKIWDYCCQVLSLPP 968
K ++V M+KC S + +V +HFRE+V R + CQVL LPP
Sbjct: 951 KTVKVWMIKCCFGRYSLSRIVLEHFREEVNSIR*Q*GHSPCQVLRLPP 808
Score = 29.0 bits (57), Expect(5) = 8e-048
Identities = 12/15 (80%), Positives = 12/15 (80%)
Frame = -2 / -1
Query: 516 SMNFLRSRSRYSKTR 472
SMNF RS SRYS TR
Sbjct: 347 SMNFFRSVSRYSNTR 303
Score = 29.0 bits (57), Expect(5) = 8e-048
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -2 / -1
Query: 951 KSGKQLTPGQLCSFGVP 901
+S ++LTPG CS GVP
Sbjct: 794 QSRRELTPGHCCSVGVP 744
>emb|X97317.1|MSCDC2MSF M.sativa mRNA for cdc2 kinase homologue, cdc2MsF
Length = 1285
Score = 228 bits (493), Expect(4) = e-105
Identities = 86/134 (64%), Positives = 107/134 (79%)
Frame = +2 / +2
Query: 542 PTLIKSYLYQILRGVAYCHSHRVLHRDLKPQNLLIDRRTNALKLADFGLARAFGIPVRTF 721
P IK +YQ+ +GVA+CH H +LHRDLKP NLL+DR+T LK+AD GLARAF +P++ +
Sbjct: 422 PPTIKGLMYQLCKGVAFCHGHGILHRDLKPHNLLMDRKTMMLKIADLGLARAFTVPLKKY 601
Query: 722 THEVVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRIL 901
THE++TLWYRAPE+LLGA YS VD+WSV CIFAE+V + LFPGDSE+ +L IFR+L
Sbjct: 602 THEILTLWYRAPEVLLGATHYSMAVDMWSVACIFAELVTKTALFPGDSELQQLLHIFRLL 781
Query: 902 GTPNEQSWPGVSCL 943
GTPNE WPGVS L
Sbjct: 782 GTPNEDVWPGVSKL 823
Score = 95.9 bits (203), Expect(5) = 5e-040
Identities = 47/85 (55%), Positives = 56/85 (65%)
Frame = -2 / -2
Query: 834 TISAKIQPTDHTSTGVEYCLAPSRISGALYHNVTTS*VNVRTGIPNALAKPKSASFSALV 655
T SAK+Q TDH ST +E +AP R SGALYH V+ S*V +G ALA P+SA S +V
Sbjct: 714 TSSAKMQATDHISTAIE*WVAPKRTSGALYHRVSIS*VYFLSGTVKALASPRSAILSIIV 535
Query: 654 RRSINKF*GFKSR*RTL*EWQYATP 580
RSI + *GFKS + * WQ ATP
Sbjct: 534 FRSIKRL*GFKSLCKIP*PWQKATP 460
Score = 90.4 bits (191), Expect(4) = e-105
Identities = 34/60 (56%), Positives = 49/60 (81%)
Frame = +2 / +2
Query: 239 AMEQYEKVEKIGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISLLKEMN 418
A E +EK+EK+GEGTYG VY+A +KAT + +ALKK RL ++DEGVP T +RE+S+L+ ++
Sbjct: 95 AKEAFEKLEKVGEGTYGKVYRAREKATGKIVALKKTRLHEDDEGVPPTTLREVSILRMLS 274
Score = 80.3 bits (169), Expect(4) = e-105
Identities = 29/51 (56%), Positives = 42/51 (82%)
Frame = +2 / +2
Query: 962 FPRWQAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLE 1114
+P+W Q L+ VP L+ AG+DLLS+ML+YEPSKR++A++A+EH YF DL+
Sbjct: 839 YPQWGPQSLSKAVPGLEEAGVDLLSQMLQYEPSKRLSAKKAMEHPYFDDLD 991
Score = 54.7 bits (113), Expect(5) = 5e-040
Identities = 28/57 (49%), Positives = 31/57 (54%)
Frame = -2 / -2
Query: 408 LRREISRMAVDGTPSSSCSRRIFXXXXXXXXXXXXXLYTTPYVPSPIFSTFSYCSIA 238
L+ E SR V GTPSSS RR+F L T PYVPSP FS+FS S A
Sbjct: 264 LKIETSRSVVGGTPSSSS*RRVFFNATIFPVAFSLAL*TFPYVPSPTFSSFSNASFA 94
Score = 41.4 bits (84), Expect(5) = 5e-040
Identities = 25/50 (50%), Positives = 27/50 (54%)
Frame = -2 / -2
Query: 1113 SRSLKYSCSSACLAVILLLGSYRSILERRSNPAGSRFGTTVARS*ACHLG 964
SRS K CS A A L GSY SI E +S PA S+ GT R * H G
Sbjct: 990 SRSSK*GCSIAFFADSRLEGSYCSI*ESKSTPASSKPGTAFERL*GPH*G 841
Score = 36.8 bits (74), Expect(4) = e-105
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +2 / +2
Query: 467 IYLVFEYLDLDLKKFMDSCPEFAKN 541
+YLVFEY+D DLKKF+ S + +N
Sbjct: 341 LYLVFEYMDTDLKKFIRSFRQTGQN 415
Score = 29.5 bits (58), Expect(5) = 5e-040
Identities = 12/15 (80%), Positives = 13/15 (86%)
Frame = -2 / -2
Query: 513 MNFLRSRSRYSKTRY 469
MNFLRS S YSKT+Y
Sbjct: 387 MNFLRSVSIYSKTKY 343
Score = 21.7 bits (41), Expect(5) = 5e-040
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = -2 / -2
Query: 936 LTPGQLCSFGVP 901
LTPG S GVP
Sbjct: 816 LTPGHTSSLGVP 781
>gb|AF302013.1| Medicago sativa subsp. x varia CDK-activating kinase (cak) mRNA,
complete cds
Length = 1590
Score = 141 bits (303), Expect(4) = 1e-080
Identities = 56/106 (52%), Positives = 70/106 (66%)
Frame = +2 / +2
Query: 635 NLLIDRRTNALKLADFGLARAFGIPVRTFTHEVVTLWYRAPEILLGARQYSTPVDVWSVG 814
N L+ LKLADFGLAR FG P R FTH+V WYRAPE+L G +QY VDVW+
Sbjct: 539 NNLLIGPNGQLKLADFGLARIFGSPDRRFTHQVFARWYRAPELLFGTKQYGPGVDVWAAA 718
Query: 815 CIFAEMVNQKPLFPGDSEIDELFKIFRILGTPNEQSWPGVSCLPDF 952
CIFAE++ ++P G S+ID+L KIF GTP+ WP + LPD+
Sbjct: 719 CIFAELLLRRPFLQGSSDIDQLGKIFAAFGTPSPSQWPDMVYLPDY 856
Score = 107 bits (228), Expect(4) = 1e-080
Identities = 44/87 (50%), Positives = 64/87 (73%)
Frame = +2 / +2
Query: 245 EQYEKVEKIGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISLLKEMNHG 424
++Y K E +GEGTYGVVYKA+D T +T+A+KKIR+ ++ EGV TA+REI LLKE+
Sbjct: 155 DRYLKREVLGEGTYGVVYKAIDTQTGQTVAIKKIRIGKQKEGVNFTALREIKLLKELKDP 334
Query: 425 NIVRLHDVVHSEKRIYLVFEYLDLDLK 505
NI+ L D + ++LVFE+++ DL+
Sbjct: 335 NIIELIDCFPHKGNLHLVFEFMETDLE 415
Score = 54.7 bits (113), Expect(4) = 1e-080
Identities = 21/35 (60%), Positives = 26/35 (74%)
Frame = +2 / +2
Query: 542 PTLIKSYLYQILRGVAYCHSHRVLHRDLKPQNLLI 646
P IKSYL L+G+A+CH +LHRD+KP NLLI
Sbjct: 449 PGDIKSYLQMTLKGLAHCHKKWILHRDMKPNNLLI 553
Score = 49.6 bits (102), Expect(4) = 1e-080
Identities = 21/42 (50%), Positives = 26/42 (61%)
Frame = +2 / +2
Query: 977 AQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYF 1102
A L ++ P LDLLSKM Y+P RI+ +QALEH YF
Sbjct: 878 APPLRSLFPMATDDALDLLSKMFTYDPKDRISVQQALEHRYF 1003
>emb|X97314.1|MSCDC2MSC M.sativa mRNA for cdc2 kinase homologue, cdc2MsC
Length = 1789
Score = 116 bits (248), Expect(6) = 5e-074
Identities = 42/75 (56%), Positives = 57/75 (76%)
Frame = +2 / +3
Query: 722 THEVVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRIL 901
T+ V+TLWYR PE+LLG +Y VD+WSVGCIFAE+++ KP+FPG E ++L KIF +
Sbjct: 678 TNRVITLWYRPPELLLGTTRYGPAVDMWSVGCIFAELLHGKPIFPGKDEPEQLNKIFELC 857
Query: 902 GTPNEQSWPGVSCLP 946
G P+E +WPGV+ P
Sbjct: 858 GAPDEVNWPGVTKTP 902
Score = 99.1 bits (210), Expect(6) = 5e-074
Identities = 39/78 (50%), Positives = 59/78 (75%)
Frame = +2 / +3
Query: 224 SHWGHAMEQYEKVEKIGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISL 403
S +++ +EK+E+IGEGTYG+VY A + T E +ALKKIR++ E EG P TAIREI +
Sbjct: 135 SRGSRSVDCFEKLEQIGEGTYGMVYMAREIETGEIVALKKIRMDNEREGFPITAIREIKI 314
Query: 404 LKEMNHGNIVRLHDVVHS 457
LK+++H N+++L ++V S
Sbjct: 315 LKKLHHENVIKLKEIVTS 368
Score = 55.1 bits (114), Expect(6) = 5e-074
Identities = 21/37 (56%), Positives = 27/37 (72%)
Frame = +2 / +3
Query: 551 IKSYLYQILRGVAYCHSHRVLHRDLKPQNLLIDRRTN 661
IK Y+ Q+L G+ YCH ++VLHRD+K NLLID N
Sbjct: 507 IKCYMRQLLTGLHYCHVNQVLHRDIKGSNLLIDNEGN 617
Score = 40.9 bits (83), Expect(6) = 5e-074
Identities = 18/39 (46%), Positives = 25/39 (64%)
Frame = +2 / +3
Query: 986 LATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYF 1102
L V + D L+LL KML +P++RI A+ AL+ EYF
Sbjct: 945 LREVFRHFDRHALELLEKMLTLDPAQRIPAKDALDAEYF 1061
Score = 28.1 bits (55), Expect(6) = 5e-074
Identities = 11/12 (91%), Positives = 12/12 (100%)
Frame = +2 / +3
Query: 665 LKLADFGLARAF 700
LKLADFGLAR+F
Sbjct: 618 LKLADFGLARSF 653
Score = 26.7 bits (52), Expect(6) = 5e-074
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +2 / +3
Query: 467 IYLVFEYLDLDLKKFMD 517
IY+VFEY+D DL D
Sbjct: 426 IYMVFEYMDHDLTGLAD 476
>gb|L07042.1|ALFERKMAP Medicago sativa MAP kinase MsERK1 mRNA, complete cds
Length = 1529
Score = 95.5 bits (202), Expect(5) = 1e-054
Identities = 34/65 (52%), Positives = 48/65 (73%)
Frame = +2 / +3
Query: 722 THEVVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRIL 901
T VVT WYRAPE+LL + Y+ +DVWSVGCIF E++++KPLFPG + +L + ++
Sbjct: 738 TEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQLRLLMELI 917
Query: 902 GTPNE 916
GTP+E
Sbjct: 918 GTPSE 932
Score = 60.6 bits (126), Expect(5) = 1e-054
Identities = 26/66 (39%), Positives = 41/66 (62%)
Frame = +2 / +3
Query: 269 IGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISLLKEMNHGNIVRLHDV 448
IG+G YG+V A + TNE +A+KKI +++ +REI LL+ M+H N+V + D+
Sbjct: 282 IGKGAYGIVCSAHNSETNEHVAVKKIANAFDNKIDAKRTLREIKLLRHMDHENVVAIRDI 461
Query: 449 VHSEKR 466
V +R
Sbjct: 462 VPPPQR 479
Score = 55.1 bits (114), Expect(5) = 1e-054
Identities = 21/30 (70%), Positives = 25/30 (83%)
Frame = +2 / +3
Query: 560 YLYQILRGVAYCHSHRVLHRDLKPQNLLID 649
+LYQILRG+ Y HS VLHRDLKP NLL++
Sbjct: 582 FLYQILRGLKYIHSANVLHRDLKPSNLLLN 671
Score = 49.6 bits (102), Expect(5) = 1e-054
Identities = 20/58 (34%), Positives = 29/58 (50%)
Frame = +2 / +3
Query: 965 PRWQAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLEVVQ*PAKC 1138
P ++ Q P++ P +DL+ KML ++P KRIT AL H Y L + C
Sbjct: 984 PPYRRQSFQEKFPHVHPEAIDLVEKMLTFDPRKRITVEDALAHPYLTSLHDISDEPVC 1157
Score = 22.1 bits (42), Expect(5) = 1e-054
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +2 / +3
Query: 665 LKLADFGLAR 694
LK+ DFGLAR
Sbjct: 684 LKICDFGLAR 713
>emb|X66469.1|MSMSK7 M.sativa mRNA msk7 for map kinase
Length = 1512
Score = 95.5 bits (202), Expect(5) = 1e-054
Identities = 34/65 (52%), Positives = 48/65 (73%)
Frame = +2 / +1
Query: 722 THEVVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRIL 901
T VVT WYRAPE+LL + Y+ +DVWSVGCIF E++++KPLFPG + +L + ++
Sbjct: 736 TEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIFMELMDRKPLFPGRDHVHQLRLLMELI 915
Query: 902 GTPNE 916
GTP+E
Sbjct: 916 GTPSE 930
Score = 60.6 bits (126), Expect(5) = 1e-054
Identities = 26/66 (39%), Positives = 41/66 (62%)
Frame = +2 / +1
Query: 269 IGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISLLKEMNHGNIVRLHDV 448
IG+G YG+V A + TNE +A+KKI +++ +REI LL+ M+H N+V + D+
Sbjct: 280 IGKGAYGIVCSAHNSETNEHVAVKKIANAFDNKIDAKRTLREIKLLRHMDHENVVAIRDI 459
Query: 449 VHSEKR 466
V +R
Sbjct: 460 VPPPQR 477
Score = 55.1 bits (114), Expect(5) = 1e-054
Identities = 21/30 (70%), Positives = 25/30 (83%)
Frame = +2 / +1
Query: 560 YLYQILRGVAYCHSHRVLHRDLKPQNLLID 649
+LYQILRG+ Y HS VLHRDLKP NLL++
Sbjct: 580 FLYQILRGLKYIHSANVLHRDLKPSNLLLN 669
Score = 49.6 bits (102), Expect(5) = 1e-054
Identities = 20/58 (34%), Positives = 29/58 (50%)
Frame = +2 / +1
Query: 965 PRWQAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLEVVQ*PAKC 1138
P ++ Q P++ P +DL+ KML ++P KRIT AL H Y L + C
Sbjct: 982 PPYRRQSFQEKFPHVHPEAIDLVEKMLTFDPRKRITVEDALAHPYLTSLHDISDEPVC 1155
Score = 22.1 bits (42), Expect(5) = 1e-054
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +2 / +1
Query: 665 LKLADFGLAR 694
LK+ DFGLAR
Sbjct: 682 LKICDFGLAR 711
>emb|X82270.1|MSMMK4 M.sativa MMK4 mRNA for protein kinase
Length = 1695
Score = 95.9 bits (203), Expect(4) = 2e-053
Identities = 34/65 (52%), Positives = 48/65 (73%)
Frame = +2 / +1
Query: 722 THEVVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRIL 901
T VVT WYRAPE+LL + Y++ +DVWSVGCIF E++N+KPLFPG + ++ + +L
Sbjct: 763 TEYVVTRWYRAPELLLNSSDYTSAIDVWSVGCIFMELMNKKPLFPGKDHVHQMRLLTELL 942
Query: 902 GTPNE 916
GTP +
Sbjct: 943 GTPTD 957
Score = 58.8 bits (122), Expect(4) = 2e-053
Identities = 24/61 (39%), Positives = 37/61 (60%)
Frame = +2 / +1
Query: 269 IGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISLLKEMNHGNIVRLHDV 448
IG G YG+V L+ TNE +A+KKI ++ +REI LL+ ++H N++ L DV
Sbjct: 307 IGRGAYGIVCSLLNTETNELVAVKKIANAFDNHMDAKRTLREIKLLRHLDHENVIGLRDV 486
Query: 449 V 451
+
Sbjct: 487 I 489
Score = 53.8 bits (111), Expect(4) = 2e-053
Identities = 19/30 (63%), Positives = 25/30 (83%)
Frame = +2 / +1
Query: 560 YLYQILRGVAYCHSHRVLHRDLKPQNLLID 649
+LYQILRG+ Y HS ++HRDLKP NLL++
Sbjct: 607 FLYQILRGLRYIHSANIIHRDLKPSNLLLN 696
Score = 52.8 bits (109), Expect(4) = 2e-053
Identities = 21/52 (40%), Positives = 32/52 (61%)
Frame = +2 / +1
Query: 965 PRWQAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLEVV 1120
P++ Q L V P++ P +DL+ KML +P++RIT +AL H Y + L V
Sbjct: 1012 PQYPRQPLNRVFPHVHPLAIDLVDKMLTIDPTRRITVEEALAHPYLEKLHDV 1167
>emb|X82268.1|MSMMK2 M.sativa MMK2 mRNA for protein kinase
Length = 1565
Score = 87.7 bits (185), Expect(5) = 6e-052
Identities = 31/67 (46%), Positives = 46/67 (68%)
Frame = +2 / +1
Query: 722 THEVVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRIL 901
T VVT WYRAPE+LL Y+ +D+WSVGCI E+V ++PLFPG + +L + ++
Sbjct: 865 TEYVVTRWYRAPELLLNCSDYTAAIDIWSVGCILGEIVTRQPLFPGRDYVHQLRLVTELI 1044
Query: 902 GTPNEQS 922
G+P++ S
Sbjct: 1045 GSPDDAS 1065
Score = 56.5 bits (117), Expect(5) = 6e-052
Identities = 21/69 (30%), Positives = 40/69 (57%)
Frame = +2 / +1
Query: 260 VEKIGEGTYGVVYKALDKATNETIALKKIRLEQEDEGVPSTAIREISLLKEMNHGNIVRL 439
+ +G G YG+V A++ T E +A+KKI ++ +REI LL+ M+H N++ +
Sbjct: 400 IRSVGRGAYGIVCAAVNAETREEVAIKKIGNAFDNRIDAKRTLREIKLLRHMDHENVMSI 579
Query: 440 HDVVHSEKR 466
D++ ++
Sbjct: 580 KDIIRPPQK 606
Score = 54.7 bits (113), Expect(5) = 6e-052
Identities = 23/59 (38%), Positives = 33/59 (55%)
Frame = +2 / +1
Query: 965 PRWQAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLEVVQ*PAKCA 1141
P++ Q+ + PN+ P +DLL KML ++PSKRI +AL H Y L + CA
Sbjct: 1114 PQYPKQNFSARFPNMSPGAVDLLEKMLIFDPSKRIKVDEALCHPYMAPLHDINEEPVCA 1290
Score = 52.8 bits (109), Expect(5) = 6e-052
Identities = 19/30 (63%), Positives = 25/30 (83%)
Frame = +2 / +1
Query: 560 YLYQILRGVAYCHSHRVLHRDLKPQNLLID 649
++YQ+LRG+ Y HS VLHRDLKP NLL++
Sbjct: 709 FVYQLLRGLKYVHSANVLHRDLKPSNLLLN 798
Score = 22.1 bits (42), Expect(5) = 6e-052
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +2 / +1
Query: 665 LKLADFGLAR 694
LK+ DFGLAR
Sbjct: 811 LKIGDFGLAR 840
>emb|AJ224336.1|MSAJ4336 Medicago sativa mRNA for MAP kinase, complete CDS
Length = 1442
Score = 94.1 bits (199), Expect(6) = 1e-051
Identities = 34/66 (51%), Positives = 47/66 (71%)
Frame = +2 / +1
Query: 722 THEVVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRIL 901
T VVT WYRAPE+LL +Y+ +DVWSVGCI E++ ++PLFPG + +L I +L
Sbjct: 625 TEYVVTRWYRAPELLLNCSEYTAAIDVWSVGCILMEIIRREPLFPGKDYVQQLALITELL 804
Query: 902 GTPNEQ 919
G+PNE+
Sbjct: 805 GSPNEE 822
Score = 53.8 bits (111), Expect(6) = 1e-051
Identities = 23/58 (39%), Positives = 29/58 (50%)
Frame = +2 / +1
Query: 965 PRWQAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLEVVQ*PAKC 1138
P Q A P++ P LDL KML ++PSKRIT +AL H Y L + C
Sbjct: 874 PHVDKQPFAERFPDMSPLALDLAEKMLVFDPSKRITVEEALNHPYMSSLHEINEEPVC 1047
Score = 53.8 bits (111), Expect(6) = 1e-051
Identities = 20/29 (68%), Positives = 24/29 (82%)
Frame = +2 / +1
Query: 560 YLYQILRGVAYCHSHRVLHRDLKPQNLLI 646
+LYQ+LRG+ Y HS VLHRDLKP NLL+
Sbjct: 469 FLYQLLRGLKYIHSANVLHRDLKPSNLLL 555
Score = 35.4 bits (71), Expect(6) = 1e-051
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = +2 / +1
Query: 260 VEKIGEGTYGVVYKALDKATNETIALKKI 346
++ +G G YG+V A + TNE +A+KKI
Sbjct: 160 LQPVGRGAYGIVCCATNSDTNEGVAIKKI 246
Score = 30.9 bits (61), Expect(6) = 1e-051
Identities = 9/22 (40%), Positives = 17/22 (77%)
Frame = +2 / +1
Query: 386 IREISLLKEMNHGNIVRLHDVV 451
+REI LL M+H N++++ D++
Sbjct: 286 LREIKLLCHMDHDNVIKIKDII 351
Score = 22.1 bits (42), Expect(6) = 1e-051
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +2 / +1
Query: 665 LKLADFGLAR 694
LK+ DFGLAR
Sbjct: 571 LKICDFGLAR 600
>emb|X97316.1|MSCDC2MSE M.sativa mRNA for cdc2 kinase homologue, cdc2MsE
Length = 1490
Score = 78.0 bits (164), Expect(6) = 5e-051
Identities = 28/43 (65%), Positives = 37/43 (86%)
Frame = +2 / +3
Query: 731 VVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPG 859
VVT+W RAPE+LLGA+ Y++ VD+W+VGCIFAE++ KPLF G
Sbjct: 423 VVTIWCRAPELLLGAKHYTSAVDMWAVGCIFAELLTLKPLFQG 551
Score = 55.1 bits (114), Expect(6) = 5e-051
Identities = 21/27 (77%), Positives = 24/27 (88%)
Frame = +2 / +3
Query: 1025 DLLSKMLRYEPSKRITARQALEHEYFK 1105
DLLSKML Y+P +RITA QA+EHEYFK
Sbjct: 759 DLLSKMLEYDPKRRITAAQAMEHEYFK 839
Score = 49.6 bits (102), Expect(6) = 5e-051
Identities = 17/36 (47%), Positives = 27/36 (75%)
Frame = +2 / +3
Query: 539 NPTLIKSYLYQILRGVAYCHSHRVLHRDLKPQNLLI 646
N +KS L+Q+L G+ Y HS+ ++HRD+KP N+L+
Sbjct: 216 NQYTVKSILWQLLNGLNYLHSNWIVHRDVKPSNILV 323
Score = 42.3 bits (86), Expect(6) = 5e-051
Identities = 17/36 (47%), Positives = 26/36 (72%)
Frame = +2 / +3
Query: 341 KIRLEQEDEGVPSTAIREISLLKEMNHGNIVRLHDV 448
K + ++ +GV TAIREI LL+E+ H N+V+L +V
Sbjct: 3 KFKQSKDGDGVSPTAIREIMLLREITHENVVKLVNV 110
Score = 39.1 bits (79), Expect(6) = 5e-051
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = +2 / +3
Query: 854 PGDSEIDELFKIFRILGTPNEQSWPGVSCLP 946
P ++D+L KIF++LG P + WP ++ LP
Sbjct: 570 PNPFQLDQLDKIFKVLGHPTLEKWPSLAHLP 662
Score = 23.5 bits (45), Expect(6) = 5e-051
Identities = 7/17 (41%), Positives = 12/17 (70%)
Frame = +2 / +3
Query: 452 HSEKRIYLVFEYLDLDL 502
H++ +YL F+Y + DL
Sbjct: 120 HTDMSLYLAFDYAEHDL 170
>emb|X68411.1|AMMSK1A A.medicago MSK-1 mRNA for protein kinase
Length = 1613
Score = 91.3 bits (193), Expect(4) = 8e-032
Identities = 30/63 (47%), Positives = 49/63 (77%)
Frame = +2 / +1
Query: 731 VVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILGTP 910
+ + +YRAPE++ GA +Y+T +DVWSVGC+ AE++ +PLFPG+ +D+L +I ++LGTP
Sbjct: 772 ICSRYYRAPELIFGATEYTTAIDVWSVGCVLAELLLGQPLFPGERGVDQLVEIIKVLGTP 951
Query: 911 NEQ 919
+
Sbjct: 952 TRE 960
Score = 46.0 bits (94), Expect(4) = 8e-032
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = +2 / +1
Query: 608 VLHRDLKPQNLLIDRRTNALKLADFGLAR 694
V HRD+KPQNLL++ T+ +KL DFG A+
Sbjct: 652 VCHRDIKPQNLLVNPHTHQVKLCDFGSAK 738
Score = 35.0 bits (70), Expect(4) = 8e-032
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = +2 / +1
Query: 269 IGEGTYGVVYKALDKATNETIALKKI 346
+G G++GVV++A T ET+A+KK+
Sbjct: 307 VGHGSFGVVFQAKCLETGETVAIKKV 384
Score = 25.4 bits (49), Expect(4) = 8e-032
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = +2 / +1
Query: 551 IKSYLYQILRGVAYCH 598
+K Y YQI R ++Y H
Sbjct: 592 VKLYTYQIFRALSYIH 639
>emb|X68409.1|AMMSK3A A.medicago MSK-3 mRNA for protein kinase
Length = 1800
Score = 89.5 bits (189), Expect(4) = 1e-031
Identities = 28/63 (44%), Positives = 48/63 (76%)
Frame = +2 / +1
Query: 731 VVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILGTP 910
+ + +YRAPE++ GA +Y+T +D+WS GC+ E++ +PLFPG+S +D+L +I ++LGTP
Sbjct: 940 ICSRYYRAPELIFGATEYTTAIDIWSAGCVLGELLLGQPLFPGESGVDQLVEIIKVLGTP 1119
Query: 911 NEQ 919
+
Sbjct: 1120 TRE 1128
Score = 46.4 bits (95), Expect(4) = 1e-031
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = +2 / +1
Query: 608 VLHRDLKPQNLLIDRRTNALKLADFGLAR 694
V HRD+KPQNLL++ T+ LK+ DFG A+
Sbjct: 820 VCHRDIKPQNLLVNPHTHQLKICDFGSAK 906
Score = 35.0 bits (70), Expect(4) = 1e-031
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = +2 / +1
Query: 269 IGEGTYGVVYKALDKATNETIALKKI 346
+G G++GVV++A T ET+A+KK+
Sbjct: 475 VGHGSFGVVFQAKCLETGETVAIKKV 552
Score = 26.3 bits (51), Expect(4) = 1e-031
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2 / +1
Query: 551 IKSYLYQILRGVAYCHS 601
+K Y YQI R +AY H+
Sbjct: 760 VKLYSYQICRALAYIHN 810
>emb|X68410.1|AMMSK2A A.medicago MSK-2 mRNA for protein kinase
Length = 1543
Score = 85.8 bits (181), Expect(4) = 3e-031
Identities = 27/63 (42%), Positives = 47/63 (74%)
Frame = +2 / +1
Query: 731 VVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILGTP 910
+ + +YRAPE++ GA +Y++ +D+WS GC+ E++ +PLFPG S +D+L +I ++LGTP
Sbjct: 757 ICSRYYRAPELIFGATEYTSAIDIWSAGCVLGELLLGQPLFPGASGVDQLVEIIKVLGTP 936
Query: 911 NEQ 919
+
Sbjct: 937 TRE 945
Score = 46.9 bits (96), Expect(4) = 3e-031
Identities = 18/29 (62%), Positives = 23/29 (79%)
Frame = +2 / +1
Query: 608 VLHRDLKPQNLLIDRRTNALKLADFGLAR 694
V HRD+KPQNLL++ T+ LKL DFG A+
Sbjct: 637 VSHRDIKPQNLLVNPHTHQLKLCDFGSAK 723
Score = 36.3 bits (73), Expect(4) = 3e-031
Identities = 13/32 (40%), Positives = 22/32 (68%)
Frame = +2 / +1
Query: 251 YEKVEKIGEGTYGVVYKALDKATNETIALKKI 346
Y +G+G++GVV++A T ET+A+KK+
Sbjct: 274 YMAERAVGQGSFGVVFQAKCLETGETVAIKKV 369
Score = 26.3 bits (51), Expect(4) = 3e-031
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2 / +1
Query: 551 IKSYLYQILRGVAYCHS 601
+K Y YQI R +AY H+
Sbjct: 577 VKLYSYQICRSLAYIHN 627
>emb|AX774637.1| Sequence 2 from Patent WO03035856
Length = 1866
Score = 91.3 bits (193), Expect(3) = 3e-030
Identities = 29/63 (46%), Positives = 49/63 (77%)
Frame = +2 / +3
Query: 731 VVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILGTP 910
+ + +YRAPE++ GA +Y+T +D+WS GC+ AE++ +PLFPG+S +D+L +I ++LGTP
Sbjct: 1059 ICSRYYRAPELIFGATEYTTAIDIWSTGCVMAELLLGQPLFPGESGVDQLVEIIKVLGTP 1238
Query: 911 NEQ 919
+
Sbjct: 1239 TRE 1247
Score = 46.9 bits (96), Expect(3) = 3e-030
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = +2 / +3
Query: 608 VLHRDLKPQNLLIDRRTNALKLADFGLAR 694
+ HRD+KPQNLL++ T+ LKL DFG A+
Sbjct: 939 ICHRDIKPQNLLVNPHTHQLKLCDFGSAK 1025
Score = 26.7 bits (52), Expect(3) = 3e-030
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2 / +3
Query: 551 IKSYLYQILRGVAYCHS 601
+K Y YQI R +AY H+
Sbjct: 879 VKLYTYQICRALAYIHN 929
>gb|AF432225.1| Medicago sativa GSK-3-like protein MsK4 mRNA, complete cds
Length = 1850
Score = 91.3 bits (193), Expect(3) = 3e-030
Identities = 29/63 (46%), Positives = 49/63 (77%)
Frame = +2 / +3
Query: 731 VVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILGTP 910
+ + +YRAPE++ GA +Y+T +D+WS GC+ AE++ +PLFPG+S +D+L +I ++LGTP
Sbjct: 1059 ICSRYYRAPELIFGATEYTTAIDIWSTGCVMAELLLGQPLFPGESGVDQLVEIIKVLGTP 1238
Query: 911 NEQ 919
+
Sbjct: 1239 TRE 1247
Score = 46.9 bits (96), Expect(3) = 3e-030
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = +2 / +3
Query: 608 VLHRDLKPQNLLIDRRTNALKLADFGLAR 694
+ HRD+KPQNLL++ T+ LKL DFG A+
Sbjct: 939 ICHRDIKPQNLLVNPHTHQLKLCDFGSAK 1025
Score = 26.7 bits (52), Expect(3) = 3e-030
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2 / +3
Query: 551 IKSYLYQILRGVAYCHS 601
+K Y YQI R +AY H+
Sbjct: 879 VKLYTYQICRALAYIHN 929
>emb|AJ295939.1|MSA295939 Medicago sativa mRNA for wound-induced GSK-3-like protein (wig gene)
Length = 1980
Score = 83.1 bits (175), Expect(4) = 1e-029
Identities = 27/63 (42%), Positives = 48/63 (76%)
Frame = +2 / +3
Query: 731 VVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILGTP 910
+ + +YRAPE++ GA +Y+T +D+WSVGC+ AE++ + +F G+S +D+L +I ++LGTP
Sbjct: 1098 ICSRYYRAPELIFGATEYTTAIDMWSVGCVLAELLLGQAMFLGESGVDQLVEIIKVLGTP 1277
Query: 911 NEQ 919
+
Sbjct: 1278 TRE 1286
Score = 46.4 bits (95), Expect(4) = 1e-029
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = +2 / +3
Query: 608 VLHRDLKPQNLLIDRRTNALKLADFGLAR 694
V HRD+KPQNLL++ +T LK+ DFG A+
Sbjct: 978 VCHRDIKPQNLLVNPQTRQLKICDFGSAK 1064
Score = 35.0 bits (70), Expect(4) = 1e-029
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = +2 / +3
Query: 269 IGEGTYGVVYKALDKATNETIALKKI 346
+G G++GVV++A TNE +A+KK+
Sbjct: 633 VGTGSFGVVFQAKCLETNEAVAIKKV 710
Score = 26.7 bits (52), Expect(4) = 1e-029
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = +2 / +3
Query: 551 IKSYLYQILRGVAYCH 598
++ Y YQILRG+ Y H
Sbjct: 918 VQLYTYQILRGLNYLH 965
>gb|CO514154.1|CO514154 s13dSG75H0800064_156970 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 555
Score = 65.7 bits (137), Expect(4) = 7e-027
Identities = 22/42 (52%), Positives = 31/42 (73%)
Frame = +2 / +2
Query: 722 THEVVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKP 847
T VVT WYRAPE+LL +Y+ +D+WSVGCI E++ ++P
Sbjct: 296 TEYVVTRWYRAPELLLNCSEYTAAIDIWSVGCILGEVMTRQP 421
Score = 54.2 bits (112), Expect(4) = 7e-027
Identities = 20/30 (66%), Positives = 25/30 (83%)
Frame = +2 / +2
Query: 560 YLYQILRGVAYCHSHRVLHRDLKPQNLLID 649
+LYQ+LRG+ Y HS VLHRDLKP NLL++
Sbjct: 140 FLYQLLRGLKYVHSANVLHRDLKPSNLLLN 229
Score = 28.6 bits (56), Expect(4) = 7e-027
Identities = 10/26 (38%), Positives = 17/26 (65%)
Frame = +2 / +3
Query: 845 PLFPGDSEIDELFKIFRILGTPNEQS 922
PLFPG + +L I ++G+P++ S
Sbjct: 420 PLFPGKDYVHQLKLITELIGSPDDAS 497
Score = 22.1 bits (42), Expect(4) = 7e-027
Identities = 8/10 (80%), Positives = 9/10 (90%)
Frame = +2 / +2
Query: 665 LKLADFGLAR 694
LK+ DFGLAR
Sbjct: 242 LKIGDFGLAR 271
>gb|CO512589.1|CO512589 s13dSG13A0100001_121104 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 572
Score = 81.3 bits (171), Expect(2) = 2e-026
Identities = 28/56 (50%), Positives = 42/56 (75%)
Frame = +2 / +2
Query: 749 RAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFPGDSEIDELFKIFRILGTPNE 916
RAPE+LL + Y++ +DVWSVGCIF E++N+KPLFPG + ++ + +LGTP +
Sbjct: 5 RAPELLLNSSDYTSAIDVWSVGCIFMELMNKKPLFPGKDHVHQMRLLTELLGTPTD 172
Score = 53.3 bits (110), Expect(2) = 2e-026
Identities = 21/52 (40%), Positives = 32/52 (61%)
Frame = +2 / +2
Query: 965 PRWQAQDLATVVPNLDPAGLDLLSKMLRYEPSKRITARQALEHEYFKDLEVV 1120
P++ Q L V P++ P +DL+ KML +P++RIT +AL H Y + L V
Sbjct: 227 PQYPRQSLNRVFPHVHPLAIDLVDKMLTIDPTRRITVEEALAHPYLEKLHDV 382
>gb|CO515142.1|CO515142 s13dSG40H0100016_399149 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 336
Score = 64.8 bits (135), Expect(3) = 2e-022
Identities = 20/42 (47%), Positives = 33/42 (78%)
Frame = +2 / +1
Query: 731 VVTLWYRAPEILLGARQYSTPVDVWSVGCIFAEMVNQKPLFP 856
+ + +YRAPE++ GA +Y+T +D+WS GC+ AE++ +PLFP
Sbjct: 211 ICSRYYRAPELIFGATEYTTAIDIWSTGCVMAELLLGQPLFP 336
Score = 46.9 bits (96), Expect(3) = 2e-022
Identities = 17/29 (58%), Positives = 23/29 (79%)
Frame = +2 / +1
Query: 608 VLHRDLKPQNLLIDRRTNALKLADFGLAR 694
+ HRD+KPQNLL++ T+ LKL DFG A+
Sbjct: 91 ICHRDIKPQNLLVNPHTHQLKLCDFGSAK 177
Score = 26.7 bits (52), Expect(3) = 2e-022
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2 / +1
Query: 551 IKSYLYQILRGVAYCHS 601
+K Y YQI R +AY H+
Sbjct: 31 VKLYTYQICRALAYIHN 81
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,591,451
Number of Sequences: 7669
Number of extensions: 126529
Number of successful extensions: 1083
Number of sequences better than 1.0e-020: 22
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 369919592
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)