TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2418879.2.2
(816 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY560003.1| Medicago sativa S-adenosylmethionine synthas... 490 e-139
gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular tr... 364 e-102
gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular tr... 235 7e-063
gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular tr... 233 2e-062
>gb|AY560003.1| Medicago sativa S-adenosylmethionine synthase mRNA, complete cds
Length = 1173
Score = 490 bits (1064), Expect = e-139
Identities = 201/215 (93%), Positives = 206/215 (95%)
Frame = -1 / +1
Query: 762 ESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKA 583
E+FLFTSESVNEGHPDKLCDQ+SDAVLDACL QD DSKVACETCTKTNMVMVFGEITTKA
Sbjct: 4 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDVDSKVACETCTKTNMVMVFGEITTKA 183
Query: 582 SVDYEKIVRDTCREIGFTSDDVGLDADRCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAG 403
VDYEKIVRDTCR+IGF SDDVGLDAD CKVLVNIEQQSPDIAQGVHGH TKRPEEIGAG
Sbjct: 184 KVDYEKIVRDTCRKIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAG 363
Query: 402 DQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKDGTCAWLRPDGKTQVTVEYVNEGG 223
DQGHMFGYATDETPELMPLSHVLATKLGARLTEVRK+GTC WLRPDGKTQVTVEY N+ G
Sbjct: 364 DQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKNGTCPWLRPDGKTQVTVEYYNDNG 543
Query: 222 AMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIK 118
AMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIK
Sbjct: 544 AMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIK 648
Score = 371 bits (804), Expect = e-104
Identities = 175/218 (80%), Positives = 184/218 (84%)
Frame = +2 / -2
Query: 104 TGMTGLMTCSLRSAAISSLVTVSSCWVEMSTVCTRTGTMAPPSFTYSTVTWVLPSGLSQA 283
+G+TG +TCSLRSAAISSLVTVSSC VEMSTV TRTGTMAP S YSTVT VLPSGL+Q
Sbjct: 662 SGITGFITCSLRSAAISSLVTVSSC*VEMSTVWTRTGTMAPLSL*YSTVT*VLPSGLNQG 483
Query: 284 QVPSLRTSVRRAPSLVASTWLSGISSGVSSVAYPNMWPWSPAPISSGRFVKCPCTPCAMS 463
VP LRTSVRRAPSLVA TWL GI+SGVSSVA PNM PWSPAPISSG FV+ PCTP A+S
Sbjct: 482 HVPFLRTSVRRAPSLVARTWLKGINSGVSSVA*PNM*PWSPAPISSGLFVR*PCTP*AIS 303
Query: 464 GDCCSMFTSTLQRSASSPTSSEVNPISRQVSRTIFS*STLAFVVISPKTITMFVLVQVSQ 643
G CCSM T TLQ SAS PTSSE NPI QVSRTIFS*STLA VVISPKTITM VLVQVS
Sbjct: 302 GLCCSMLTRTLQLSASRPTSSETNPIFLQVSRTIFS*STLALVVISPKTITMLVLVQVSH 123
Query: 644 ATLLSGS*ARQASSTASDTWSHSLSGCPSFTDSEVNRK 757
ATLLS S +RQASSTAS+ S SLSG PSFTDSEVNRK
Sbjct: 122 ATLLSTSCSRQASSTASEI*SQSLSG*PSFTDSEVNRK 9
Score = 96.4 bits (204), Expect(2) = 2e-026
Identities = 40/123 (32%), Positives = 76/123 (61%)
Frame = +1 / -1
Query: 241 LHRHLGLAVGPEPGAGAVLADLRQARAELGGQHVAQRHQLGGLVGGVPEHVALVAGADLL 420
L+ L L + +P A +LA+L + +LG +++AQRHQ L+ + +HV LV +LL
Sbjct: 525 LNSDLSLTIRSQPRACTILANLSETSTKLGCKNMAQRHQFRSLISSIAKHVTLVTSTNLL 346
Query: 421 GPLREVPVHALRDVGGLLLDVHQHLAAVGVEPHIVGGEPDLAAGVAHDLLVVHARLRGDL 600
++ ++ L ++ LLL+V ++LA V ++ +I+ +P+L+ G+ H+ L+V+ L DL
Sbjct: 345 RSFCKMTMYTLSNIRTLLLNVDKNLAVVSIKTYIIRNKPNLSTGITHNFLIVNLSLGCDL 166
Query: 601 AEN 609
++
Sbjct: 165 TKD 157
Score = 36.8 bits (74), Expect(2) = 2e-026
Identities = 14/31 (45%), Positives = 22/31 (70%)
Frame = +1 / -1
Query: 670 QAGVQHRVRHLVAQLVWVPLVHGLRGEQEAL 762
+A + H +R L+ +LV + L+HGLRGE + L
Sbjct: 96 KASI*HSIRDLITKLVGMTLIHGLRGE*KGL 4
>gb|CO514605.1|CO514605 s13dSG42G0700056_327870 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 600
Score = 364 bits (789), Expect = e-102
Identities = 147/162 (90%), Positives = 156/162 (96%)
Frame = -1 / +2
Query: 771 MAAESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEIT 592
MA E+FL+TSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNMVMVFGEIT
Sbjct: 113 MATETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEIT 292
Query: 591 TKASVDYEKIVRDTCREIGFTSDDVGLDADRCKVLVNIEQQSPDIAQGVHGHFTKRPEEI 412
TKA+VDYEKIVR+TCR IGF SDDVGLDAD CKVLVNIEQQSPDIAQGVHGH T+RPEEI
Sbjct: 293 TKANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTERPEEI 472
Query: 411 GAGDQGHMFGYATDETPELMPLSHVLATKLGARLTEVRKDGT 286
GAGDQGHMFGYATDETPELMPL+HVLATKLGA+LTEVRK+GT
Sbjct: 473 GAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKNGT 598
Score = 251 bits (542), Expect = 1e-067
Identities = 117/157 (74%), Positives = 125/157 (79%)
Frame = +2 / -1
Query: 287 VPSLRTSVRRAPSLVASTWLSGISSGVSSVAYPNMWPWSPAPISSGRFVKCPCTPCAMSG 466
VP LRTSV AP+LVA TW+ GISSGVSSVAYPNM P SPAPISSG V+ P TP A+SG
Sbjct: 597 VPFLRTSVSLAPNLVARTWVKGISSGVSSVAYPNM*P*SPAPISSGLSVRWP*TP*AISG 418
Query: 467 DCCSMFTSTLQRSASSPTSSEVNPISRQVSRTIFS*STLAFVVISPKTITMFVLVQVSQA 646
CCSM T TLQ SAS PTSSE P+ V RTIFS*ST AFVVISPKTITM V VQVS A
Sbjct: 417 LCCSMLTRTLQLSASRPTSSETKPMFLHVLRTIFS*STFAFVVISPKTITMLVFVQVSHA 238
Query: 647 TLLSGS*ARQASSTASDTWSHSLSGCPSFTDSEVNRK 757
TLLSGS +RQAS TAS+ WS SLSGCPSFTDS+V RK
Sbjct: 237 TLLSGSCSRQASRTASEIWSQSLSGCPSFTDSDVYRK 127
>gb|CO514747.1|CO514747 s13dSG55F1100095_328154 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 435
Score = 235 bits (507), Expect = 7e-063
Identities = 96/104 (92%), Positives = 100/104 (96%)
Frame = -1 / +1
Query: 765 AESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTK 586
AE+FLFTSESVNEGHPDKLCDQ+SDAVLDACL QDPDSK ACETCTKTNMVMVFGEITTK
Sbjct: 124 AETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKGACETCTKTNMVMVFGEITTK 303
Query: 585 ASVDYEKIVRDTCREIGFTSDDVGLDADRCKVLVNIEQQSPDIA 454
A+VDYEKIVRDTCR IGF SDDVGLDAD+CKVLVNIEQQSPDIA
Sbjct: 304 ANVDYEKIVRDTCRTIGFISDDVGLDADKCKVLVNIEQQSPDIA 435
Score = 173 bits (372), Expect = 3e-044
Identities = 79/101 (78%), Positives = 86/101 (85%)
Frame = +2 / -2
Query: 455 AMSGDCCSMFTSTLQRSASSPTSSEVNPISRQVSRTIFS*STLAFVVISPKTITMFVLVQ 634
A+SG CCSM T TL SAS PTSSE+NP+ R VSRTIFS*STLA VVISPKT+TM VLV
Sbjct: 434 AISGLCCSMLTKTLHLSASRPTSSEMNPMVRHVSRTIFS*STLALVVISPKTMTMLVLVH 255
Query: 635 VSQATLLSGS*ARQASSTASDTWSHSLSGCPSFTDSEVNRK 757
VSQA LLSGS +RQASSTAS+ WSHSLSG PSFTDS+VNRK
Sbjct: 254 VSQAPLLSGSCSRQASSTASEIWSHSLSG*PSFTDSDVNRK 132
>gb|CO516632.1|CO516632 s13dSG66E0200007_446592 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 425
Score = 233 bits (503), Expect = 2e-062
Identities = 94/104 (90%), Positives = 99/104 (95%)
Frame = -1 / +2
Query: 771 MAAESFLFTSESVNEGHPDKLCDQVSDAVLDACLAQDPDSKVACETCTKTNMVMVFGEIT 592
MA E+FL+TSESVNEGHPDKLCDQ+SDAVLDACL QDPDSKVACETCTKTNMVMVFGEIT
Sbjct: 113 MATETFLYTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEIT 292
Query: 591 TKASVDYEKIVRDTCREIGFTSDDVGLDADRCKVLVNIEQQSPD 460
TKA+VDYEKIVR+TCR IGF SDDVGLDAD CKVLVNIEQQSPD
Sbjct: 293 TKANVDYEKIVRNTCRNIGFVSDDVGLDADNCKVLVNIEQQSPD 424
Score = 163 bits (350), Expect = 3e-041
Identities = 75/99 (75%), Positives = 79/99 (79%)
Frame = +2 / -3
Query: 461 SGDCCSMFTSTLQRSASSPTSSEVNPISRQVSRTIFS*STLAFVVISPKTITMFVLVQVS 640
SG CCSM T TLQ SAS PTSSE P+ V RTIFS*ST AFVVISPKTITM V VQVS
Sbjct: 423 SGLCCSMLTRTLQLSASRPTSSETKPMFLHVLRTIFS*STFAFVVISPKTITMLVFVQVS 244
Query: 641 QATLLSGS*ARQASSTASDTWSHSLSGCPSFTDSEVNRK 757
ATLLSGS +RQAS TAS+ WS SLSGCPSFTDS+V RK
Sbjct: 243 HATLLSGSCSRQASRTASEIWSQSLSGCPSFTDSDVYRK 127
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,750,170
Number of Sequences: 7669
Number of extensions: 48552
Number of successful extensions: 591
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 212161630
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)