TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2418879.2.1
(1083 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY560003.1| Medicago sativa S-adenosylmethionine synthas... 386 e-124
gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular tr... 189 1e-065
gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted... 111 2e-025
>gb|AY560003.1| Medicago sativa S-adenosylmethionine synthase mRNA, complete cds
Length = 1173
Score = 386 bits (838), Expect(2) = e-124
Identities = 162/190 (85%), Positives = 170/190 (89%)
Frame = +3 / +1
Query: 6 TQVTVEYVNEGGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPERYLDEKTI 185
TQVTVEY N+ GAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPE+YLD KTI
Sbjct: 508 TQVTVEYYNDNGAMVPVRVHTVLISTQHDETVTNDEIAADLKEHVIKPVIPEKYLDSKTI 687
Query: 186 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYVARQA 365
FHLNPSGRFVIGGPHGDAGLTGRKIIIDTY KDPTKVDRSGAY+ RQA
Sbjct: 688 FHLNPSGRFVIGGPHGDAGLTGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQA 867
Query: 366 AKSIVASGLARRCLVQVSYAIGVPEPLSVFVDSYGTGTIPDKEILKIVKENFDFRPGMIT 545
AKSIVASGLARRC+VQVSYAIGVPEPLSVFVD+YGTG IPDKEIL IVK+NFDFRPGMI+
Sbjct: 868 AKSIVASGLARRCIVQVSYAIGVPEPLSVFVDTYGTGKIPDKEILNIVKQNFDFRPGMIS 1047
Query: 546 INLDLKKGGN 575
INLDL +GGN
Sbjct: 1048 INLDLLRGGN 1077
Score = 155 bits (334), Expect(2) = 9e-061
Identities = 71/90 (78%), Positives = 75/90 (83%)
Frame = -3 / -2
Query: 271 VSMMILRPVRPASPWGPPMTKRPDGLRWKMVFSSRYLSGMTGLMTCSLRSAAISSLVTVS 92
VSMMI PVRPASP GPPMTKRP+G + KMV SRY SG+TG +TCSLRSAAISSLVTVS
Sbjct: 773 VSMMIFLPVRPASP*GPPMTKRPEGFK*KMVLESRYFSGITGFITCSLRSAAISSLVTVS 594
Query: 91 SCWVEMSTVCTRTGTMAPPSFTYSTVTWVL 2
SC VEMSTV TRTGTMAP S YSTVT VL
Sbjct: 593 SC*VEMSTVWTRTGTMAPLSL*YSTVT*VL 504
Score = 96.8 bits (205), Expect(2) = 9e-061
Identities = 41/56 (73%), Positives = 47/56 (83%)
Frame = -3 / -2
Query: 574 LPPFLRSRLMVIIPGLKSKFSLTIFRISLSGMVPVP*ESTNTDRGSGTPMA*DTCT 407
LPP RSRL+ I+PGLKSKF TI RISLSG+ PVP STNTD+GSGTP+A*+TCT
Sbjct: 1076 LPPLKRSRLIEIMPGLKSKFCFTILRISLSGIFPVPYVSTNTDKGSGTPIA*ETCT 909
Score = 73.0 bits (153), Expect(2) = e-124
Identities = 27/33 (81%), Positives = 30/33 (90%)
Frame = +3 / +1
Query: 558 LKKGGNRFIKTAAYGHFGRDDADFTWEVVKPLK 656
L+ G RF+KTAAYGHFGR+DADFTWEVVKPLK
Sbjct: 1063 LRGGNGRFLKTAAYGHFGREDADFTWEVVKPLK 1161
>gb|CO514483.1|CO514483 s13dSG43C0600040_327626 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 505
Score = 189 bits (407), Expect(2) = 1e-065
Identities = 78/87 (89%), Positives = 84/87 (96%)
Frame = +3 / +3
Query: 315 KDPTKVDRSGAYVARQAAKSIVASGLARRCLVQVSYAIGVPEPLSVFVDSYGTGTIPDKE 494
KDPTKVDRSGAY+ RQAAKSIVA+GLARRC+VQVSYAIGVPEPLSVFVDSYGTG IPDKE
Sbjct: 24 KDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYAIGVPEPLSVFVDSYGTGKIPDKE 203
Query: 495 ILKIVKENFDFRPGMITINLDLKKGGN 575
IL IVKE+FDFRPGMI+INLDLK+GGN
Sbjct: 204 ILNIVKESFDFRPGMISINLDLKRGGN 284
Score = 96.4 bits (204), Expect(2) = 3e-027
Identities = 43/57 (75%), Positives = 48/57 (84%)
Frame = -3 / -1
Query: 577 LLPPFLRSRLMVIIPGLKSKFSLTIFRISLSGMVPVP*ESTNTDRGSGTPMA*DTCT 407
L PP LRSRL+ IIPGLKSK S T+ RISLSG+ PVP*ESTNTD GSGTP+A*+T T
Sbjct: 286 LFPPLLRSRLIEIIPGLKSKDSFTMLRISLSGIFPVP*ESTNTDNGSGTPIA*ET*T 116
Score = 75.8 bits (159), Expect(2) = 1e-065
Identities = 28/30 (93%), Positives = 29/30 (96%)
Frame = +3 / +3
Query: 567 GGNRFIKTAAYGHFGRDDADFTWEVVKPLK 656
G NRF+KTAAYGHFGRDDADFTWEVVKPLK
Sbjct: 279 GNNRFLKTAAYGHFGRDDADFTWEVVKPLK 368
Score = 40.5 bits (82), Expect(2) = 3e-027
Identities = 20/28 (71%), Positives = 20/28 (71%)
Frame = -3 / -1
Query: 649 GLTTSQVKSASSRPKWPYAAVLMNLLPP 566
G TTS VKSASS PK P AAV NLL P
Sbjct: 361 GFTTSHVKSASSLPK*P*AAVFKNLLFP 278
>gb|AW698879.1|AW698879 r109 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 205
Score = 111 bits (237), Expect = 2e-025
Identities = 48/66 (72%), Positives = 51/66 (77%)
Frame = +3 / +3
Query: 246 TGRKIIIDTYXXXXXXXXXXXXXKDPTKVDRSGAYVARQAAKSIVASGLARRCLVQVSYA 425
TGRKIIIDTY KDPTKVDRSGAY+ RQAAKSIVA+GLARRC+VQVSYA
Sbjct: 3 TGRKIIIDTYGGWGAHGGGAFSGKDPTKVDRSGAYIVRQAAKSIVANGLARRCIVQVSYA 182
Query: 426 IGVPEP 443
IGVP P
Sbjct: 183 IGVPXP 200
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,286,263
Number of Sequences: 7669
Number of extensions: 73696
Number of successful extensions: 2181
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 291259917
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)