TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2405341.2.2
(1164 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AX776636.1| Sequence 5 from Patent WO02102841 82 2e-051
emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulatio... 82 2e-051
emb|AX776635.1| Sequence 4 from Patent WO02102841 79 4e-049
emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulatio... 79 4e-049
gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated... 68 2e-032
gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular tr... 79 2e-031
gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular tr... 62 2e-029
gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular tr... 83 1e-026
gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular tr... 105 3e-026
gb|CO515446.1|CO515446 s13dSG50H0600060_417951 Glandular tr... 71 4e-023
>emb|AX776636.1| Sequence 5 from Patent WO02102841
Length = 3339
Score = 82.2 bits (173), Expect(5) = 2e-051
Identities = 35/64 (54%), Positives = 45/64 (70%)
Frame = +2 / +3
Query: 302 KSEQLDWDARINIILGAAKGLSYLHHDCSPRIIHRDIKSSNILLDGSFEARVSDFGLAKL 481
K + LDW R++I LGAA+GL+YLH +IHRD+KSSNILLD S A+V+DFG +K
Sbjct: 2304 KRKILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFSKY 2483
Query: 482 LEDE 493
E
Sbjct: 2484 APQE 2495
Score = 69.3 bits (145), Expect(5) = 2e-051
Identities = 31/85 (36%), Positives = 52/85 (61%)
Frame = +2 / +3
Query: 485 EDEESHITTIVAGTFGYLAPEYMQFGRATEKTDVYSFGVLVLEILSGKRPTDASFIEKGL 664
++ +S+++ V GT GYL PEY + + +EK+DV+SFGV++LEI+SG+ P +
Sbjct: 2490 QEGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEW 2669
Query: 665 NIVGWLNFLAGENREREIVDLNCEG 739
++V W ++ EIVD +G
Sbjct: 2670 SLVEWAKPYIRASKVDEIVDPGIKG 2744
Score = 52.4 bits (108), Expect(5) = 2e-051
Identities = 19/51 (37%), Positives = 33/51 (64%)
Frame = +2 / +3
Query: 161 FDRELEILGSVKHRYLVNLRGYCNSPSSKLLIYDYLQGGSLDEVLHEKSEQ 313
FD EL +L +++H LV L GYCN ++L+Y ++ GSL + L+ ++ +
Sbjct: 2154 FDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYGEASK 2306
Score = 37.3 bits (75), Expect(5) = 2e-051
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = +1 / +3
Query: 34 TMDEENIIGAGGFGTVYKLQMDDGNVLALK 123
T + +IG GGFG+VY+ +DDG +A+K
Sbjct: 2028 TEQYKTLIGEGGFGSVYRGTLDDGQEVAVK 2117
Score = 30.4 bits (60), Expect(5) = 2e-051
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +2 / +3
Query: 737 GVHTETLDALLSLAKQCVSSLPEERPTMHRVVQMLESDVI 856
G H E L ++ +A QC+ RP M +V+ LE +I
Sbjct: 2745 GYHAEALWRVVEVALQCLEPYSTYRPCMVDIVRELEDALI 2864
>emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulation receptor kinase (nork gene), wild
type allele DMI2
Length = 3339
Score = 82.2 bits (173), Expect(5) = 2e-051
Identities = 35/64 (54%), Positives = 45/64 (70%)
Frame = +2 / +3
Query: 302 KSEQLDWDARINIILGAAKGLSYLHHDCSPRIIHRDIKSSNILLDGSFEARVSDFGLAKL 481
K + LDW R++I LGAA+GL+YLH +IHRD+KSSNILLD S A+V+DFG +K
Sbjct: 2304 KRKILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAKVADFGFSKY 2483
Query: 482 LEDE 493
E
Sbjct: 2484 APQE 2495
Score = 69.3 bits (145), Expect(5) = 2e-051
Identities = 31/85 (36%), Positives = 52/85 (61%)
Frame = +2 / +3
Query: 485 EDEESHITTIVAGTFGYLAPEYMQFGRATEKTDVYSFGVLVLEILSGKRPTDASFIEKGL 664
++ +S+++ V GT GYL PEY + + +EK+DV+SFGV++LEI+SG+ P +
Sbjct: 2490 QEGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEW 2669
Query: 665 NIVGWLNFLAGENREREIVDLNCEG 739
++V W ++ EIVD +G
Sbjct: 2670 SLVEWAKPYIRASKVDEIVDPGIKG 2744
Score = 52.4 bits (108), Expect(5) = 2e-051
Identities = 19/51 (37%), Positives = 33/51 (64%)
Frame = +2 / +3
Query: 161 FDRELEILGSVKHRYLVNLRGYCNSPSSKLLIYDYLQGGSLDEVLHEKSEQ 313
FD EL +L +++H LV L GYCN ++L+Y ++ GSL + L+ ++ +
Sbjct: 2154 FDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYGEASK 2306
Score = 37.3 bits (75), Expect(5) = 2e-051
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = +1 / +3
Query: 34 TMDEENIIGAGGFGTVYKLQMDDGNVLALK 123
T + +IG GGFG+VY+ +DDG +A+K
Sbjct: 2028 TEQYKTLIGEGGFGSVYRGTLDDGQEVAVK 2117
Score = 30.4 bits (60), Expect(5) = 2e-051
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +2 / +3
Query: 737 GVHTETLDALLSLAKQCVSSLPEERPTMHRVVQMLESDVI 856
G H E L ++ +A QC+ RP M +V+ LE +I
Sbjct: 2745 GYHAEALWRVVEVALQCLEPYSTYRPCMVDIVRELEDALI 2864
>emb|AX776635.1| Sequence 4 from Patent WO02102841
Length = 3378
Score = 78.5 bits (165), Expect(5) = 4e-049
Identities = 34/64 (53%), Positives = 44/64 (68%)
Frame = +2 / +1
Query: 302 KSEQLDWDARINIILGAAKGLSYLHHDCSPRIIHRDIKSSNILLDGSFEARVSDFGLAKL 481
K + LDW R++I LGA +GL+ LH P +IHRD+KSSNILLD S A+V+DFG +K
Sbjct: 2323 KRKILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFSKY 2502
Query: 482 LEDE 493
E
Sbjct: 2503 APQE 2514
Score = 69.3 bits (145), Expect(5) = 4e-049
Identities = 31/85 (36%), Positives = 52/85 (61%)
Frame = +2 / +1
Query: 485 EDEESHITTIVAGTFGYLAPEYMQFGRATEKTDVYSFGVLVLEILSGKRPTDASFIEKGL 664
++ +S+++ V GT GYL PEY + + +EK+DV+SFGV++LEI+SG+ P +
Sbjct: 2509 QEGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEW 2688
Query: 665 NIVGWLNFLAGENREREIVDLNCEG 739
++V W ++ EIVD +G
Sbjct: 2689 SLVEWAKPYIRASKVDEIVDPGIKG 2763
Score = 52.4 bits (108), Expect(5) = 4e-049
Identities = 19/51 (37%), Positives = 33/51 (64%)
Frame = +2 / +1
Query: 161 FDRELEILGSVKHRYLVNLRGYCNSPSSKLLIYDYLQGGSLDEVLHEKSEQ 313
FD EL +L +++H LV L GYCN ++L+Y ++ GSL + L+ ++ +
Sbjct: 2173 FDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYGEASK 2325
Score = 33.1 bits (66), Expect(5) = 4e-049
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = +1 / +1
Query: 34 TMDEENIIGAGGFGTVYKLQMDDGNVLALK 123
T + +I GGFG+VY+ +DDG +A+K
Sbjct: 2047 TEQYKTLICEGGFGSVYRGTLDDGQEVAVK 2136
Score = 30.4 bits (60), Expect(5) = 4e-049
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +2 / +1
Query: 737 GVHTETLDALLSLAKQCVSSLPEERPTMHRVVQMLESDVI 856
G H E L ++ +A QC+ RP M +V+ LE +I
Sbjct: 2764 GYHAEALWRVVEVALQCLEPYSTYRPCMVDIVRELEDALI 2883
>emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulation receptor kinase (nork gene),
mutant allele nn1
Length = 3337
Score = 78.5 bits (165), Expect(5) = 4e-049
Identities = 34/64 (53%), Positives = 44/64 (68%)
Frame = +2 / +1
Query: 302 KSEQLDWDARINIILGAAKGLSYLHHDCSPRIIHRDIKSSNILLDGSFEARVSDFGLAKL 481
K + LDW R++I LGA +GL+ LH P +IHRD+KSSNILLD S A+V+DFG +K
Sbjct: 2302 KRKILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAKVADFGFSKY 2481
Query: 482 LEDE 493
E
Sbjct: 2482 APQE 2493
Score = 69.3 bits (145), Expect(5) = 4e-049
Identities = 31/85 (36%), Positives = 52/85 (61%)
Frame = +2 / +1
Query: 485 EDEESHITTIVAGTFGYLAPEYMQFGRATEKTDVYSFGVLVLEILSGKRPTDASFIEKGL 664
++ +S+++ V GT GYL PEY + + +EK+DV+SFGV++LEI+SG+ P +
Sbjct: 2488 QEGDSYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGREPLNIKRPRIEW 2667
Query: 665 NIVGWLNFLAGENREREIVDLNCEG 739
++V W ++ EIVD +G
Sbjct: 2668 SLVEWAKPYIRASKVDEIVDPGIKG 2742
Score = 52.4 bits (108), Expect(5) = 4e-049
Identities = 19/51 (37%), Positives = 33/51 (64%)
Frame = +2 / +1
Query: 161 FDRELEILGSVKHRYLVNLRGYCNSPSSKLLIYDYLQGGSLDEVLHEKSEQ 313
FD EL +L +++H LV L GYCN ++L+Y ++ GSL + L+ ++ +
Sbjct: 2152 FDNELNLLSAIQHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLYGEASK 2304
Score = 33.1 bits (66), Expect(5) = 4e-049
Identities = 13/30 (43%), Positives = 20/30 (66%)
Frame = +1 / +1
Query: 34 TMDEENIIGAGGFGTVYKLQMDDGNVLALK 123
T + +I GGFG+VY+ +DDG +A+K
Sbjct: 2026 TEQYKTLICEGGFGSVYRGTLDDGQEVAVK 2115
Score = 30.4 bits (60), Expect(5) = 4e-049
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = +2 / +1
Query: 737 GVHTETLDALLSLAKQCVSSLPEERPTMHRVVQMLESDVI 856
G H E L ++ +A QC+ RP M +V+ LE +I
Sbjct: 2743 GYHAEALWRVVEVALQCLEPYSTYRPCMVDIVRELEDALI 2862
>gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated receptor-like kinase
mRNA, complete cds
Length = 1798
Score = 68.4 bits (143), Expect(3) = 2e-032
Identities = 25/48 (52%), Positives = 39/48 (81%)
Frame = +2 / +1
Query: 497 SHITTIVAGTFGYLAPEYMQFGRATEKTDVYSFGVLVLEILSGKRPTD 640
+HI+T V GT GY+ PEY++ + TEK+DVYSFGVL++E+++G+ P +
Sbjct: 1030 THISTQVKGTAGYMDPEYLRTYQLTEKSDVYSFGVLLVEMMTGRHPVE 1173
Score = 63.8 bits (133), Expect(3) = 2e-032
Identities = 27/63 (42%), Positives = 41/63 (65%)
Frame = +2 / +1
Query: 302 KSEQLDWDARINIILGAAKGLSYLHHDCSPRIIHRDIKSSNILLDGSFEARVSDFGLAKL 481
+ + L+ R++I + A ++YLH IIHRDIK+SNIL+ + A+V+DFG A+L
Sbjct: 832 RGDGLEIGERLDIAIDIAHAITYLHMYTDNPIIHRDIKASNILISENLRAKVADFGFARL 1011
Query: 482 LED 490
ED
Sbjct: 1012 SED 1020
Score = 47.3 bits (97), Expect(3) = 2e-032
Identities = 18/42 (42%), Positives = 28/42 (66%)
Frame = +1 / +1
Query: 10 KDILKKLETMDEENIIGAGGFGTVYKLQMDDGNVLALKRIVK 135
++I K +N IG GGFGTVY+ +++DG ++A+KR K
Sbjct: 535 EEIYKSTAKFSSDNQIGEGGFGTVYRGKLNDGTIVAVKRAKK 660
>gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 595
Score = 78.5 bits (165), Expect(2) = 2e-031
Identities = 30/56 (53%), Positives = 41/56 (73%)
Frame = +2 / +1
Query: 314 LDWDARINIILGAAKGLSYLHHDCSPRIIHRDIKSSNILLDGSFEARVSDFGLAKL 481
LDW+ R+ I GAA+GL YLH P +I+RD+K SNILL + +++SDFGLAK+
Sbjct: 52 LDWNTRMKIAAGAARGLEYLHDKMKPPVIYRDLKCSNILLGEDYHSKLSDFGLAKV 219
Score = 72.5 bits (152), Expect(2) = 2e-031
Identities = 28/72 (38%), Positives = 46/72 (63%)
Frame = +2 / +1
Query: 491 EESHITTIVAGTFGYLAPEYMQFGRATEKTDVYSFGVLVLEILSGKRPTDASFIEKGLNI 670
+++H++T V GT+GY AP+Y G+ T K+D+YS GV +LE+++G++ D K N+
Sbjct: 232 DKTHVSTRVMGTYGYCAPDYAMTGQLTFKSDIYSIGVALLELITGRKAIDHRRPAKEQNL 411
Query: 671 VGWLNFLAGENR 706
V W L + R
Sbjct: 412 VAWARPLFRDRR 447
>gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 62.5 bits (130), Expect(3) = 2e-029
Identities = 26/56 (46%), Positives = 33/56 (58%)
Frame = +2 / +1
Query: 314 LDWDARINIILGAAKGLSYLHHDCSPRIIHRDIKSSNILLDGSFEARVSDFGLAKL 481
L W R+ I+ G AKGL YLH + +H D+K SNILL +SDFGL +L
Sbjct: 175 LTWSDRLKIMKGTAKGLVYLHEFSPKKYVHGDLKPSNILLGHDMTPYISDFGLGRL 342
Score = 53.3 bits (110), Expect(3) = 2e-029
Identities = 22/51 (43%), Positives = 30/51 (58%)
Frame = +2 / +1
Query: 155 RFFDRELEILGSVKHRYLVNLRGYCNSPSSKLLIYDYLQGGSLDEVLHEKS 307
R F E+E +G ++H + LR Y S KLLIYDY+ GSL +H K+
Sbjct: 1 REFQTEVEAIGKLRHPNIATLRAYYWSVDEKLLIYDYIPNGSLASAIHGKA 153
Score = 46.0 bits (94), Expect(3) = 2e-029
Identities = 16/35 (45%), Positives = 28/35 (80%)
Frame = +2 / +1
Query: 530 GYLAPEYMQFGRATEKTDVYSFGVLVLEILSGKRP 634
GY APE ++ + ++K DVYS+GV++LE+++G+ P
Sbjct: 457 GYQAPEALKVVKPSQKWDVYSYGVILLEMITGRLP 561
>gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 611
Score = 83.1 bits (175), Expect(2) = 1e-026
Identities = 34/74 (45%), Positives = 50/74 (67%)
Frame = +2 / +1
Query: 299 EKSEQLDWDARINIILGAAKGLSYLHHDCSPRIIHRDIKSSNILLDGSFEARVSDFGLAK 478
+ S + W+ RI + L AA+G+ YLH P IIHRDIK+SNILLD + A+VSDFGL+
Sbjct: 268 QTSTIMSWNGRIKVALDAARGIEYLHKYAQPPIIHRDIKTSNILLDSKWVAKVSDFGLSL 447
Query: 479 LLEDEESHITTIVA 520
+ ++E +++A
Sbjct: 448 MGPEDEESHLSLLA 489
Score = 51.9 bits (107), Expect(2) = 1e-026
Identities = 20/56 (35%), Positives = 34/56 (60%)
Frame = +2 / +1
Query: 134 KQMKGLDRFFDRELEILGSVKHRYLVNLRGYCNSPSSKLLIYDYLQGGSLDEVLHE 301
K+ + D F ELE L + H+ LV L G+ + ++L+Y+Y+ GSL++ LH+
Sbjct: 97 KRQEDTDSAFVNELESLSRLHHKNLVKLLGFYEDKNGRILVYEYMNNGSLNDHLHK 264
>gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 596
Score = 105 bits (223), Expect(2) = 3e-026
Identities = 40/92 (43%), Positives = 65/92 (70%)
Frame = +2 / +1
Query: 395 IIHRDIKSSNILLDGSFEARVSDFGLAKLLEDEESHITTIVAGTFGYLAPEYMQFGRATE 574
++HRD+KS+NILLD F +V+DFGL++L ++ +H++T GT GY+ PEY Q + T+
Sbjct: 310 VMHRDVKSNNILLDEKFHVKVADFGLSRLFPNDVTHVSTAPQGTPGYVDPEYYQCYQLTD 489
Query: 575 KTDVYSFGVLVLEILSGKRPTDASFIEKGLNI 670
K+D YSFGV+++E++S R D + +N+
Sbjct: 490 KSDAYSFGVVLVELISSLRAVDITRHRNDVNL 585
Score = 28.6 bits (56), Expect(2) = 3e-026
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2 / +1
Query: 314 LDWDARINIILGAAKGLSYLH 376
L W R++I L A+ L+YLH
Sbjct: 238 LPWSVRLDIALETAEALAYLH 300
>gb|CO515446.1|CO515446 s13dSG50H0600060_417951 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 531
Score = 70.7 bits (148), Expect(2) = 4e-023
Identities = 28/54 (51%), Positives = 41/54 (75%)
Frame = +2 / +3
Query: 332 INIILGAAKGLSYLHHDCSPRIIHRDIKSSNILLDGSFEARVSDFGLAKLLEDE 493
+ I LGAA+GL++LHH C P II ++I S+ IL+D F+AR+ DFGLA+L+ +
Sbjct: 6 VRIGLGAARGLAWLHHGCHPPIIQQNICSNVILVDEEFDARIMDFGLARLMTSD 167
Score = 52.4 bits (108), Expect(2) = 4e-023
Identities = 21/46 (45%), Positives = 32/46 (69%)
Frame = +2 / +3
Query: 521 GTFGYLAPEYMQFGRATEKTDVYSFGVLVLEILSGKRPTDASFIEK 658
G GY+APEY A+ K DVY FGVL+LE+++G +P + + I++
Sbjct: 198 GELGYIAPEYSSTMVASLKGDVYGFGVLLLELVTGCKPLEVNNIDE 335
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,477,534
Number of Sequences: 7669
Number of extensions: 155447
Number of successful extensions: 1196
Number of sequences better than 1.0e-020: 13
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 316067544
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)