TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404928.2.2
(1151 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF512999.1| Medicago sativa TPR1 (tpr1) mRNA, complete cds 572 e-164
>gb|AF512999.1| Medicago sativa TPR1 (tpr1) mRNA, complete cds
Length = 1264
Score = 572 bits (1243), Expect = e-164
Identities = 228/279 (81%), Positives = 261/279 (93%)
Frame = -3 / +1
Query: 1149 DECIKDCDKAVERGRELRADFKMISRALTRKGTALVKLAKTSKDFDIAIETYQKALTEHR 970
++CIKDCDKAVERGRELRAD+KMI+RALTRKGTA+ K AK SKD++ IETYQKALTEHR
Sbjct: 214 EDCIKDCDKAVERGRELRADYKMIARALTRKGTAMGKTAKCSKDYEPVIETYQKALTEHR 393
Query: 969 NPDTLKKLNEAERAKKELEQQEYYDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNP 790
NPDTLKKLNEAE+AKKELEQQEY+DP LADEEREKGNE+FK+QKYPEAIKHYTE++KRNP
Sbjct: 394 NPDTLKKLNEAEKAKKELEQQEYFDPNLADEEREKGNEYFKQQKYPEAIKHYTESIKRNP 573
Query: 789 KDPRVYSNRAACYTKLGAMPEGLKDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAMETY 610
K+P+ YSNRAACYTKLGAMPEGLKDAEKC+ELDPTFTKGYTRKGA+QFFMKEY+KA+ETY
Sbjct: 574 KNPKAYSNRAACYTKLGAMPEGLKDAEKCIELDPTFTKGYTRKGAVQFFMKEYEKALETY 753
Query: 609 QAGLKHDPKNQELLDGVRRCIEQINKANRGEISQDELQERQNKAMQDPEIQNILTDPIMR 430
Q GLKHD NQELL+GVR C++QIN+ +RG+++ +EL+ERQ KAMQDPEIQNIL DP+MR
Sbjct: 754 QEGLKHDANNQELLEGVRSCVKQINRTSRGDVTPEELKERQAKAMQDPEIQNILQDPVMR 933
Query: 429 QVLNDFQENPRAAQEHLKNPGVMQKIQKLVSAGIVQMR* 313
QVL DFQENP+AAQEH KNPGVM KIQKL+SAGIVQM+*
Sbjct: 934 QVLVDFQENPKAAQEHTKNPGVMDKIQKLISAGIVQMK* 1050
Score = 200 bits (431), Expect(2) = 2e-089
Identities = 91/144 (63%), Positives = 99/144 (68%)
Frame = +2 / -3
Query: 314 HLIWTIPALTSF*IFCITPGFFRCSWAALGFSWKSFSTCLMIGSVRMFWISGSCIALFCL 493
+ IWTIPAL SF I ITPGF CS AA GFSWKS +TCL+ GS RMF ISGSCIAL CL
Sbjct: 1049 YFIWTIPALISFWILSITPGFLVCSCAAFGFSWKSTNTCLITGS*RMF*ISGSCIALACL 870
Query: 494 SCNXXXXXXXXXXXXXXXMHLLTPSSSSWFFGSCFRPA**VSMALSYSFMKN*IAPFLVY 673
S N L TPSS+SW F SCF P+ VS A SYSFMKN* APF V
Sbjct: 869 SFNSSGVTSPRLVLLICFTQLRTPSSNSWLFASCFNPSWYVSKAFSYSFMKN*TAPFRV* 690
Query: 674 PLVKVGSSSRHFSASLRPSGMAPN 745
PLVKVGSSS HFSAS +PSG+AP+
Sbjct: 689 PLVKVGSSSIHFSASFKPSGIAPS 618
Score = 157 bits (337), Expect(2) = 8e-045
Identities = 76/171 (44%), Positives = 100/171 (58%)
Frame = +1 / -2
Query: 319 HLDYPSTYKLLNLLHNSRVLQVLLGRPWVLLEII*HLSHDRVGKNVLDLWVLHSFVLSFL 498
HLD T +LL+L+H++RVL + L W+ LEI +LSH+RV K+VL+LW+LH LS L
Sbjct: 1044 HLDNSGTDQLLDLVHHTRVLGMFLCSLWIFLEINQYLSHNRVLKDVLNLWILHCLSLSLL 865
Query: 499 QFVLTYFTSVSLVNLFNAPPNTIKQFLVFRVMLQASLIGFHGLVIFFHEELNCTFPCVPF 678
Q +Y + +NL + N KQFLV +M Q L+ F L IFFHE+LN T P +
Sbjct: 864 QLFRSYIPTTGSINLLHTTSNPFKQFLVVCIMFQPFLVCFQSLFIFFHEKLNRTLPGITL 685
Query: 679 SEGGV*FKTLLCIFKTFRHGS*LGVAGSPIAVNPRILWVSLESLRVVFYCF 831
EG V TLLCI TFRH V S + ++ RI VS L V+ F
Sbjct: 684 GEGRVKLNTLLCIL*TFRHCPQFCVTCSSVTISLRIFRVSFYRLCVMLNSF 532
Score = 144 bits (309), Expect(2) = 2e-089
Identities = 74/114 (64%), Positives = 74/114 (64%)
Frame = +2 / -3
Query: 809 SV*CFIASGYFCSLKNSLPFSLSSSANFGS*YXXXXXXXXXXXXXXXXXRVSGFRCSVRA 988
SV*C IASGYFC LK S PFSLSSSA FGS Y RVSG RCSV A
Sbjct: 554 SV*CLIASGYFCCLKYSFPFSLSSSAKFGSKYSCCSSSFLAFSASLSFFRVSGLRCSVSA 375
Query: 989 FW*VSMAISKSLEVLASLTRAVPFLVSALEIILKSARSSLPLSTALSQSLMHSS 1150
FW*VSM S SLE A L AVPFLV AL IIL SA SS PLSTALSQS M SS
Sbjct: 374 FW*VSMTGS*SLEHFAVLPMAVPFLVKALAIIL*SALSSRPLSTALSQSFMQSS 213
Score = 110 bits (235), Expect(3) = 1e-035
Identities = 59/134 (44%), Positives = 85/134 (63%)
Frame = -1 / +2
Query: 968 TQTLLKS*MRLRERKKSWSNKSTMIQN*QMRRERKATSSSRSKNTQKQ*NTTRRLSRETQ 789
TQT ++ M+LR +K+ +NK+T IQ MRRE+K ++ S++ K + T+ L +ET+
Sbjct: 395 TQTP*RNSMKLRRPRKN*NNKNTSIQIWLMRREKKGMNTLSSRSILKLLSITQSL*KETR 574
Query: 788 RILGFTAIGLPATPS*EPCLKVLKMQRSVLN*TPPSLKGTQGKVQFNSS*KNMTRPWKPI 609
+IL L T + CLKV +MQRSVL+ T PS + G+V+F+ S*KNM R WK
Sbjct: 575 KILRLIVTELHVTQNWGQCLKV*RMQRSVLSLTRPSPRVIPGRVRFSFS*KNMKRLWKHT 754
Query: 608 RLA*SMTRKTRNCL 567
R *+M + TRNCL
Sbjct: 755 RKG*NMMQTTRNCL 796
Score = 58.3 bits (121), Expect(2) = 5e-021
Identities = 33/80 (41%), Positives = 48/80 (60%)
Frame = -2 / +3
Query: 1027 L*GF*YCHRDLPEGSN*ASEPRHS*KVE*G*ESEKRVGATRVL*SKISR*GERERQRVLQ 848
L* * CH DLP+ + A PRH + +* *E ++R TR+L SK *GER+R+ +L
Sbjct: 336 L*RL*TCH*DLPKSAYRAP*PRHPEETQ*S*EGQERTRTTRILRSKFG**GERKRE*IL* 515
Query: 847 GAKIPRSNKTLHGGSQEKPK 788
A++ S LH ++KP+
Sbjct: 516 AAEVS*SY*ALHRVYKKKPE 575
Score = 57.4 bits (119), Expect(2) = 5e-021
Identities = 29/57 (50%), Positives = 37/57 (64%)
Frame = -2 / +3
Query: 736 HA*RS*RCREVS*TRPHLH*RVHKERCNSILHERI*QGHGNLSGWPEA*PEKPGTA* 566
+A*R CREV * P LH ++ E C S+ HERI*+G GN+ G E * ++PG A*
Sbjct: 627 NA*RFKGCREVY*A*PDLHQGLYPEGCGSVFHERI*KGFGNIPGRVET*CKQPGIA* 797
Score = 42.3 bits (86), Expect(2) = 8e-045
Identities = 22/65 (33%), Positives = 31/65 (47%)
Frame = +1 / -2
Query: 955 KSVWVPMLS*SLLVGLDGNIKILRGLSKLD*SCSFSCQCP*NHLEISTKFSSPLHSLITV 1134
+ VWV +L LV L+ I R + SF CQ HL + + SS +SLIT+
Sbjct: 408 QGVWVTVLCKRFLVSLNDRFIIFRAFCRFTHGSSFPCQSSCYHLVVCS*LSSSFNSLITI 229
Query: 1135 LDAFI 1149
A +
Sbjct: 228 FYAIL 214
Score = 40.0 bits (81), Expect(3) = 1e-035
Identities = 20/42 (47%), Positives = 26/42 (61%)
Frame = -1 / +2
Query: 497 KKDRTKLCKTQRSRTFLPTLS*DRC*MISRRTQGRPRST*RT 372
++DR + CK QR RT TL *DR +ISR+ Q R+ RT
Sbjct: 866 RRDRLRQCKIQRFRTSFKTLL*DRYWLISRKIQRLHRNIPRT 991
Score = 32.7 bits (65), Expect(3) = 1e-035
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = -2 / +3
Query: 1144 MHQGL**GCGEGKRTSC*FQDDFKGTDKKRN 1052
+H+ L**GC + R QDD K DK+RN
Sbjct: 219 LHKRL**GC*KRTRAKSRLQDDSKSFDKERN 311
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,686,983
Number of Sequences: 7669
Number of extensions: 134171
Number of successful extensions: 829
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 312392340
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)