TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404928.2.1
(932 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF512999.1| Medicago sativa TPR1 (tpr1) mRNA, complete cds 409 e-118
>gb|AF512999.1| Medicago sativa TPR1 (tpr1) mRNA, complete cds
Length = 1264
Score = 409 bits (887), Expect(2) = e-118
Identities = 159/197 (80%), Positives = 185/197 (93%)
Frame = -1 / +1
Query: 788 FYDPKLADEEREKGNEFFKEQKYPEAIKHYTEALKRNPKDPRVYSNRAACYTKLGAMPEG 609
++DP LADEEREKGNE+FK+QKYPEAIKHYTE++KRNPK+P+ YSNRAACYTKLGAMPEG
Sbjct: 460 YFDPNLADEEREKGNEYFKQQKYPEAIKHYTESIKRNPKNPKAYSNRAACYTKLGAMPEG 639
Query: 608 LKDAEKCLELDPTFTKGYTRKGAIQFFMKEYDKAVETYQAGLKHDPNNQELLDGVKRCVE 429
LKDAEKC+ELDPTFTKGYTRKGA+QFFMKEY+KA+ETYQ GLKHD NNQELL+GV+ CV+
Sbjct: 640 LKDAEKCIELDPTFTKGYTRKGAVQFFMKEYEKALETYQEGLKHDANNQELLEGVRSCVK 819
Query: 428 QINKANRGEISQDELQERQNKAMQDPEIQNILTDPIMRQVLIDFQENPSAAQEHLKNPGV 249
QIN+ +RG+++ +EL+ERQ KAMQDPEIQNIL DP+MRQVL+DFQENP AAQEH KNPGV
Sbjct: 820 QINRTSRGDVTPEELKERQAKAMQDPEIQNILQDPVMRQVLVDFQENPKAAQEHTKNPGV 999
Query: 248 MQKIQKLVSAGIVQMR* 198
M KIQKL+SAGIVQM+*
Sbjct: 1000 MDKIQKLISAGIVQMK* 1050
Score = 225 bits (486), Expect(3) = 5e-068
Identities = 101/144 (70%), Positives = 109/144 (75%)
Frame = +1 / -3
Query: 199 HLI*TIPALTSF*IFCITPGFFRCSWAALGFSWKSIRTCRMIGSVRMFWISGSCIALFCL 378
+ I TIPAL SF I ITPGF CS AA GFSWKS TC + GS RMF ISGSCIAL CL
Sbjct: 1049 YFIWTIPALISFWILSITPGFLVCSCAAFGFSWKSTNTCLITGS*RMF*ISGSCIALACL 870
Query: 379 SCNSSWLISPLLALLICSTHLLTPSSSSWLFGSCFKPAW*VSTALSYSFMKN*IAPFLVY 558
S NSS + SP L LLIC T L TPSS+SWLF SCF P+W VS A SYSFMKN* APF V
Sbjct: 869 SFNSSGVTSPRLVLLICFTQLRTPSSNSWLFASCFNPSWYVSKAFSYSFMKN*TAPFRV* 690
Query: 559 PLVKVGSSSRHFSASLRPSGMAPN 630
PLVKVGSSS HFSAS +PSG+AP+
Sbjct: 689 PLVKVGSSSIHFSASFKPSGIAPS 618
Score = 164 bits (353), Expect = 1e-041
Identities = 81/181 (44%), Positives = 105/181 (58%)
Frame = +3 / -2
Query: 174 KANHRIARSSHLNYPSTYKLLNLLHNPGVLQVLLGRTWVLLEIY*NLSHDRVGKNVLDLW 353
K+ H + HL+ T +LL+L+H+ VL + L W+ LEI LSH+RV K+VL+LW
Sbjct: 1074 KSCHLPSNLLHLDNSGTDQLLDLVHHTRVLGMFLCSLWIFLEINQYLSHNRVLKDVLNLW 895
Query: 354 VLHSFVLSFLQFVLAYFTSVGLVDLFNTPLNTIKQFLVVRVMLQASLVGFHGLVIFFHEE 533
+LH LS LQ +Y + G ++L +T N KQFLVV +M Q LV F L IFFHE+
Sbjct: 894 ILHCLSLSLLQLFRSYIPTTGSINLLHTTSNPFKQFLVVCIMFQPFLVCFQSLFIFFHEK 715
Query: 534 LNCTFPRIPFGEGGVKFKTLFCVFKTFRHGS*LGVAGGPIAINPRILRVSLESLRVVFYC 713
LN T P I GEG VK TL C+ TFRH V + I+ RI RVS L V+
Sbjct: 714 LNRTLPGITLGEGRVKLNTLLCIL*TFRHCPQFCVTCSSVTISLRIFRVSFYRLCVMLNS 535
Query: 714 F 716
F
Sbjct: 534 F 532
Score = 145 bits (311), Expect = 8e-036
Identities = 80/182 (43%), Positives = 109/182 (59%)
Frame = -2 / +2
Query: 802 *SNKSFMIQNWQMRRERKATSSSRSKNTQKQ*NTTRRLSRETRRILGFIAIGPPATPS*E 623
*+NK+ IQ W MRRE+K ++ S++ K + T+ L +ETR+IL I T +
Sbjct: 446 *NNKNTSIQIWLMRREKKGMNTLSSRSILKLLSITQSL*KETRKILRLIVTELHVTQNWG 625
Query: 622 PCLKVLKTQKSVLNLTPPSPKGIRGKVQFSSS*KNMTRPWKPTRLA*SMTRTTRNCLMVL 443
CLKV + Q+SVL+LT PSP+ I G+V+FS S*KNM R WK TR *+M +TTRNCL
Sbjct: 626 QCLKV*RMQRSVLSLTRPSPRVIPGRVRFSFS*KNMKRLWKHTRKG*NMMQTTRNCLKGF 805
Query: 442 RGVLNRSTRPTEVK*ARTNCKKDRTKLCKTQRSRTFLPTLSCDRF**ISRRTQVRPRST* 263
V ++ P * + ++DR + CK QR RT TL DR+ ISR+ Q R+
Sbjct: 806 EVV*SKLIEPVVGM*LLKS*RRDRLRQCKIQRFRTSFKTLL*DRYWLISRKIQRLHRNIP 985
Query: 262 RT 257
RT
Sbjct: 986 RT 991
Score = 122 bits (260), Expect(2) = 2e-037
Identities = 71/158 (44%), Positives = 96/158 (60%)
Frame = -3 / +3
Query: 906 RI*YCYRXLPEGSDXASEPRNS*KVX*G*ESKERVRATRVL*SKIGR*GERERQRVLQGA 727
R+* C+ LP+ + A PR+ + * *E +ER R TR+L SK G *GER+R+ +L A
Sbjct: 342 RL*TCH*DLPKSAYRAP*PRHPEETQ*S*EGQERTRTTRILRSKFG**GERKRE*IL*AA 521
Query: 726 KIPRSNKTLHGGSQEKPEGS*GL*Q*GRLLHQVXXXXXXXXXXRKVS*T*PHLHQRVYEE 547
++ S LH ++KPE S*GL* +LH+ R+V * *P LHQ +Y E
Sbjct: 522 EVS*SY*ALHRVYKKKPEKS*GL**QSCMLHKTGGNA*RFKGCREVY*A*PDLHQGLYPE 701
Query: 546 RCNSVLHERI*QGRGNLPGWLEA*PEQPGTA*WC*EVC 433
C SV HERI*+G GN+PG +E * +QPG A* ++C
Sbjct: 702 GCGSVFHERI*KGFGNIPGRVET*CKQPGIA*RGSKLC 815
Score = 103 bits (220), Expect = 3e-023
Identities = 59/128 (46%), Positives = 67/128 (52%)
Frame = +2 / -1
Query: 413 WPC*SVQHTS*HHQAVPGCSGHASSQPGRFPRPCHILS*RTELHLSSYTLW*RWGQVQDT 592
W * H QA+PGC H S+ PG FP+P HILS*+TE H S Y W*R GQ Q T
Sbjct: 835 WFY*FASHNFEPLQAIPGCLHHVSTLPGMFPKPFHILS*KTEPHPSGYNPW*RSGQAQYT 656
Query: 593 FLRL*DLQAWLLTWCSRRPYCYKP*DPSGFS*EPPCSVLLLLGIFAP*RTRCLSLSPHLP 772
L +LQA C+ + YKP*D SGF C+ L A * L LSPH P
Sbjct: 655 SLHPLNLQALPPVLCNMQLCYYKP*DFSGFFL*TLCNA**LQDTSAA*SIHSLFLSPHQP 476
Query: 773 ILDHKTLV 796
LD LV
Sbjct: 475 NLDRSILV 452
Score = 48.7 bits (100), Expect(2) = 2e-037
Identities = 24/64 (37%), Positives = 34/64 (53%)
Frame = -3 / +3
Query: 390 RIARKTEQSYARPRDPEHSYRPYHATGSNRFPGEPKCGPGAPEEPRGYAEDSEACKCWDS 211
R+ +T AR RD EH RP + TG FPG+ K G +EP +D +A +C +
Sbjct: 858 RVEGETG*GNARSRDSEHPSRPCYETGIG*FPGKSKGCTGTYQEPGCDGQDPKADQCRNC 1037
Query: 210 SDEM 199
DE+
Sbjct: 1038 PDEV 1049
Score = 37.3 bits (75), Expect(3) = 5e-068
Identities = 15/19 (78%), Positives = 15/19 (78%)
Frame = +1 / -3
Query: 694 SV*CFIASGYFCSLKNSLP 750
SV*C IASGYFC LK S P
Sbjct: 554 SV*CLIASGYFCCLKYSFP 498
Score = 32.2 bits (64), Expect(2) = e-118
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = -1 / +1
Query: 902 FDIAIEXSQKALTXHRNPET 843
++ IE QKALT HRNP+T
Sbjct: 346 YEPVIETYQKALTEHRNPDT 405
Score = 29.0 bits (57), Expect(3) = 5e-068
Identities = 10/13 (76%), Positives = 10/13 (76%)
Frame = +1 / -3
Query: 841 RVSGFRCXVRAFW 879
RVSG RC V AFW
Sbjct: 407 RVSGLRCSVSAFW 369
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,809,649
Number of Sequences: 7669
Number of extensions: 118083
Number of successful extensions: 1062
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 245319867
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)