TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2193041.2.1
(877 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY205233.1| Medicago sativa 60S ribosomal protein mRNA, ... 310 1e-085
>gb|AY205233.1| Medicago sativa 60S ribosomal protein mRNA, partial cds
Length = 544
Score = 310 bits (672), Expect = 1e-085
Identities = 131/163 (80%), Positives = 147/163 (90%)
Frame = +3 / +1
Query: 72 MGIDLVAGGRNKKTKRTAPKSDDVYLKLLVKLYRFLVRRTKSNFNAVILKRLFMSKTNRP 251
MGIDL AGG++KKTKRTAPKS+D+YLKLLVKLYRFLVRRT+SNFN VILKRLFMSK N+P
Sbjct: 52 MGIDLKAGGKSKKTKRTAPKSNDIYLKLLVKLYRFLVRRTESNFNKVILKRLFMSKVNKP 231
Query: 252 PLSMRRLVRFMEGKGDQIAVIVGTVTDDKRISEVPAMKVCALRFTETARARIINAGGECL 431
PLS+ RL+R+ +GK +IAV+VG VTDD R+ EVPA+KV ALRFTE ARARI AGGECL
Sbjct: 232 PLSLSRLIRYTKGKEGKIAVVVGAVTDDIRVYEVPAIKVTALRFTEKARARIEKAGGECL 411
Query: 432 TFDQLALRAPLGQNTVLLRGPKNAREAVRHFGPAPGVPHSHTK 560
TFDQLAL+APLGQNTVLLRGPKNAREAV+HFG APGVPHS TK
Sbjct: 412 TFDQLALKAPLGQNTVLLRGPKNAREAVKHFGRAPGVPHSSTK 540
Score = 170 bits (366), Expect(2) = 2e-048
Identities = 85/124 (68%), Positives = 88/124 (70%)
Frame = -1 / -3
Query: 559 LVWL*GTPGAGPKCLTASLAFLGPLSRTVFCPSGARSASWSKVRHSPPALMILALAVSVN 380
LV L GTPGA PKCLTAS AFLGPL RTVFCP GA AS SKVRHSPPA ILALA SVN
Sbjct: 539 LVLLCGTPGARPKCLTASRAFLGPLRRTVFCPKGALRAS*SKVRHSPPAFSILALAFSVN 360
Query: 379 LSAQTFIAGTSLILLSSVTVPTITAI*SPFPSMNLTRRRIESGGRLVLLMKRRLRITALK 200
L A T IAGTS L+SSVT PT TAI FP + +SGG L LLMK LRIT LK
Sbjct: 359 LRAVTLIAGTS*TLMSSVTAPTTTAILPSFPLV*RINLESDSGGLLTLLMKSLLRITLLK 180
Query: 199 LLFV 188
LL V
Sbjct: 179 LLSV 168
Score = 37.3 bits (75), Expect(2) = 2e-048
Identities = 16/21 (76%), Positives = 17/21 (80%)
Frame = -1 / -3
Query: 133 DLGAVRLVFLLRPPATRSIPM 71
D GAVRLVFL PPA +SIPM
Sbjct: 113 DFGAVRLVFLDLPPAFKSIPM 51
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,881,149
Number of Sequences: 7669
Number of extensions: 64832
Number of successful extensions: 644
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 230691030
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)