TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192958.2.3
(1681 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like pr... 422 e-177
gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x va... 525 e-150
gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein Ms... 128 e-148
gb|CO513103.1|CO513103 s13dSG89H0300028_122132 Glandular tr... 182 5e-070
emb|Z71997.1|MSDNAJLP M.sativa mRNA for DnaJ-like protein 206 8e-061
gb|AW698418.1|AW698418 G266 glandular-haired subtracted cDN... 134 2e-032
gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDN... 123 7e-029
>emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like protein
Length = 1545
Score = 422 bits (915), Expect(3) = e-177
Identities = 165/227 (72%), Positives = 193/227 (85%)
Frame = -3 / +1
Query: 1274 DDVVHPLKVSLEDLYNGTSKKLSLSRSVLCSKCNGKGSKSGASSRCAGCQGSGFKVQIRQ 1095
+DVVH +KVSLED+YNGT+KKLSLSR+ LCSKC GKGSKSG + RC GCQG+G K+ RQ
Sbjct: 418 EDVVHSIKVSLEDVYNGTTKKLSLSRNALCSKCKGKGSKSGTAGRCFGCQGTGMKITRRQ 597
Query: 1094 LGPGMIQQMQHPCNECKGSGETISDKDRCPQCKGDKVVQEKKVLEVFVEKGMQNGQKITF 915
+G GMIQQMQH C +CKG+GE IS++DRCPQCKG+K+ QEKKVLEV VEKGMQ G KI F
Sbjct: 598 IGLGMIQQMQHVCPDCKGTGEVISERDRCPQCKGNKITQEKKVLEVHVEKGMQQGHKIVF 777
Query: 914 PGEADEAPDTVTGDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTESLCGFQFVVTHLDNRQ 735
G+ADE PDT+TGDI+FVLQ K HPKF+R+ DDL EH L+LT++LCGFQF VTHLD RQ
Sbjct: 778 EGQADELPDTITGDIVFVLQVKGHPKFRRERDDLHIEHNLSLTDALCGFQFNVTHLDGRQ 957
Query: 734 LLIKSNPGEVVKPDSFKAINDEGMPMYQRPFMKGKLYIHFSVEFPDS 594
LL+KSNPGEV+KP KAINDEGMP + RPFMKG+LYI FSV+FPDS
Sbjct: 958 LLVKSNPGEVIKPGQHKAINDEGMPQHGRPFMKGRLYIKFSVDFPDS 1098
Score = 299 bits (648), Expect(2) = e-107
Identities = 136/231 (58%), Positives = 152/231 (65%)
Frame = +1 / -2
Query: 595 ESGNSTEKWMYSLPFMNGLWYMGMPSSLIALKESGFTTSPGFDLISSCLLSK*VTTNWKP 774
ESG ST MY PFMNGL G+PSS IAL G TSPGFDL +SCL S+ VT NWKP
Sbjct: 1097 ESGKSTLNLMYRRPFMNGLPCCGIPSSFIALC*PGLMTSPGFDLTNSCLPSRCVTLNWKP 918
Query: 775 HRDSVKVRVCS*KRSSPFLLNLGCSFCWRTKMISPVTVSGASSASPGNVIFCPFCMPFST 954
R SVK+++CS RSS L N GC F +TK +SPV VSG+SSA P N I P C+PFST
Sbjct: 917 QRASVKLKLCSM*RSSRSLRNFGCPFTCKTKTMSPVIVSGSSSACPSNTIL*PCCIPFST 738
Query: 955 NTSRTFFSCTTLSPLHCGHLSLSLIVSPEPLHSLQGCCICWIIPGPNCLIWTLKPEPWQP 1134
TS TFFS L PLH GHLSLSL+ SP PL S Q CCIC I+P P CL+ P PW P
Sbjct: 737 CTSSTFFS*VILFPLH*GHLSLSLMTSPVPLQSGQTCCIC*IMPSPICLLVIFIPVPWHP 558
Query: 1135 AHLDEAPDFEPLPLHLEQRTLRDRESFFDVPLYKSSRETFRG*TTSSPRRC 1287
HL P FEP PLH E + +SFF VPLY SS+ETF TTSSP C
Sbjct: 557 KHLPAVPLFEPFPLHFEHSAFLESDSFFVVPLYTSSKETFIECTTSSPCFC 405
Score = 161 bits (346), Expect(3) = e-177
Identities = 62/74 (83%), Positives = 71/74 (95%)
Frame = -3 / +1
Query: 1610 KKSDNTRYYEILGVSKDASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQGYEVLSDPEK 1431
+KSDNT+YY+ILGVSK AS+D++KKAYRKAA+KNHPDKGGDPEKFKEL Q YEVLSDPEK
Sbjct: 79 RKSDNTKYYDILGVSKSASEDEIKKAYRKAAMKNHPDKGGDPEKFKELGQAYEVLSDPEK 258
Query: 1430 REIYDQYGEDALKE 1389
+E+YDQYGEDALKE
Sbjct: 259 KELYDQYGEDALKE 300
Score = 104 bits (222), Expect(2) = e-107
Identities = 53/73 (72%), Positives = 55/73 (75%)
Frame = +1 / -2
Query: 1390 SLRASSPYWS*ISLFSGSLRTS*P*ASSLNFSGSPPLSGWFLMAALR*AFLRSSCDASLE 1569
SLRASSPY S S FSGSL TS +SLNFSGSPPLSGWF +AA *AFL SS A E
Sbjct: 299 SLRASSPY*SYSSFFSGSLNTSYAWPNSLNFSGSPPLSGWFFIAAFL*AFLISSSLALFE 120
Query: 1570 TPRIS*YRVLSLF 1608
TPRIS*Y VLSLF
Sbjct: 119 TPRIS*YLVLSLF 81
Score = 80.3 bits (169), Expect(4) = 1e-030
Identities = 43/123 (34%), Positives = 69/123 (56%)
Frame = +3 / -1
Query: 567 QGLALLRAQRVRELHREVDVQLALHERPLVHGHAFVVDRLERIRLHNFARI*FDQQLPVI 746
Q LA + R+RE++ ++DV +LHERP + H+ ++ L +FA + FDQ LP I
Sbjct: 1125 QALAWGKKTRIREINTKLDV*ASLHERPTMLWHSLIIYSFMLTWLDDFAGVRFDQ*LPSI 946
Query: 747 QVSHNKLEATQRFSQGQGVLVEEVITXXXXXXXXXXXENKDDISSDSVRRFISFTRECDL 926
++ KLEAT+ S Q V +VIT ++K+ +S D +R FIS + E +L
Sbjct: 945 KMCDIKLEATESIS*AQIVFNVKVITLPPKLRMSLYLQDKNYVSCDCIREFISLSFEYNL 766
Query: 927 LPI 935
+ +
Sbjct: 765 VTL 757
Score = 76.2 bits (160), Expect(3) = e-177
Identities = 30/59 (50%), Positives = 41/59 (69%)
Frame = -3 / +1
Query: 593 LSPEQCKALEAVLPPKPVSQYTDMELDECEETMPYDVNIEAEMRRRQQQHQEAYDEDED 417
LSP Q LE +LP K + E+D+CEET +DVNI EM R++QQ++EAYD+D+D
Sbjct: 1105 LSPSQSLELEKILPQKTSKNLSQKEVDDCEETTLHDVNIAEEMSRKKQQYREAYDDDDD 1281
Score = 68.9 bits (144), Expect(3) = 5e-025
Identities = 46/129 (35%), Positives = 71/129 (55%)
Frame = -2 / +3
Query: 1083 NDPANAASLQRVQGFWRDNQRQG*MPTVQG**SCAGEEGS*SVCGERHAEWAEDHIPW*S 904
+D NA L +Q R + + *MP++QG + +EG+ CG+ A ++D I S
Sbjct: 609 HDSTNATRLS*LQRNRRGH**ER*MPSMQGKQDYSRKEGAGGACGKGDAAGSQDCIRRTS 788
Query: 903 **SA*HCHWRYHLCSPAEGASQVQEKG**PLLRAHPDLD*ISVWLPVCCDSLG*QAAADQ 724
**+ *+ H R+ C ++G S+V E **P +L+* S+WLPV C + +A Q
Sbjct: 789 **TP*YNHRRHSFCLASKGTSEVSEGA**PSH*TQFELN*CSLWLPV*CHTS*WKATIGQ 968
Query: 723 IKSGRSCEA 697
I+ RS +A
Sbjct: 969 IEPRRSHQA 995
Score = 44.6 bits (91), Expect(3) = 5e-025
Identities = 21/71 (29%), Positives = 35/71 (49%)
Frame = -2 / +3
Query: 1338 ILWWWKPFWRWQQ*GQKATAGR*CXXXXXXXXXXFVQWHIKEALSVPQCPLLQVQW*GFK 1159
I++W + +W W +K A R C V H KEA++ +C +L++ FK
Sbjct: 354 IIFWCRLWWWWSFTRKKTEARRRCGAFYKGFLGGCV*RHYKEAITF*ECTVLKM*RERFK 533
Query: 1158 VWSFIEVCWLP 1126
W+ +V W+P
Sbjct: 534 KWNCWKVFWMP 566
Score = 35.9 bits (72), Expect(4) = 1e-030
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = +3 / -1
Query: 1383 HSLLEGILTILVIDLSLFRVAEDLITLS 1466
HS +GI TIL+I LFR+A+ I L+
Sbjct: 306 HSFFKGIFTILIIQFFLFRIAQHFICLA 223
Score = 34.1 bits (68), Expect(4) = 1e-030
Identities = 16/41 (39%), Positives = 28/41 (68%)
Frame = +3 / -1
Query: 990 ITLALWASILVADCLSRTLALVARMLHLLDHSRPQLPDLDL 1112
++LAL ASI + + L+ + A+ +LHLL+H++ LP +L
Sbjct: 702 VSLALRASISLTNDLACSFAVRTDVLHLLNHAQSNLPSGNL 580
Score = 34.1 bits (68), Expect(3) = 5e-025
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = -1 / +2
Query: 1444 ATLKRERSMTNMVRMPSRREW 1382
A LKR+ M NMV+MP ++EW
Sbjct: 245 AILKRKNCMINMVKMPLKKEW 307
Score = 33.6 bits (67), Expect(4) = 1e-030
Identities = 16/41 (39%), Positives = 20/41 (48%)
Frame = +2 / -3
Query: 1211 ASLMCHCTNPPEKPSEDEPHHLPAVAFCPYCCHLQKGFHHQ 1333
ASL C T+PP KP + PH L A F H + H +
Sbjct: 481 ASL*CRYTHPPRKPL*NAPHLLLASVFLRVKDHHHQSLHQK 359
>gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x varia young root nodule Medicago
sativa subsp. x varia cDNA clone MsNod658, mRNA sequence
Length = 1004
Score = 525 bits (1140), Expect = e-150
Identities = 209/250 (83%), Positives = 229/250 (91%)
Frame = -3 / +3
Query: 1154 GASSRCAGCQGSGFKVQIRQLGPGMIQQMQHPCNECKGSGETISDKDRCPQCKGDKVVQE 975
GAS +CAGCQG+G KV IR LGP MIQQMQHPCNECKG+GETI+DKDRCPQCKG+KVVQE
Sbjct: 3 GASMKCAGCQGTGMKVSIRHLGPSMIQQMQHPCNECKGTGETINDKDRCPQCKGEKVVQE 182
Query: 974 KKVLEVFVEKGMQNGQKITFPGEADEAPDTVTGDIIFVLQQKEHPKFKRKGDDLFYEHTL 795
KKVLEV VEKGMQN QKITFPGEADEAPDTVTGDI+FVLQQKEHPKFKRK +DLF EHTL
Sbjct: 183 KKVLEVHVEKGMQNSQKITFPGEADEAPDTVTGDIVFVLQQKEHPKFKRKSEDLFVEHTL 362
Query: 794 TLTESLCGFQFVVTHLDNRQLLIKSNPGEVVKPDSFKAINDEGMPMYQRPFMKGKLYIHF 615
+LTE+LCGFQFV+THLD RQLLIKSNPGEVVKPDS+KAINDEGMPMYQRPFMKGKLYIHF
Sbjct: 363 SLTEALCGFQFVLTHLDGRQLLIKSNPGEVVKPDSYKAINDEGMPMYQRPFMKGKLYIHF 542
Query: 614 SVEFPDSLSPEQCKALEAVLPPKPVSQYTDMELDECEETMPYDVNIEAEMRRRQQQHQEA 435
+VEFPD+LS +Q K L+AVLP KP SQ TDME+DECEET +DVN+E E RR+QQQ QEA
Sbjct: 543 TVEFPDTLSLDQVKGLQAVLPAKPSSQLTDMEIDECEETTLHDVNMEEENRRKQQQQQEA 722
Query: 434 YDEDEDMPGG 405
YDED+DMPGG
Sbjct: 723 YDEDDDMPGG 752
Score = 390 bits (846), Expect = e-109
Identities = 173/269 (64%), Positives = 186/269 (69%)
Frame = +1 / -2
Query: 349 FYHCYQAAAYCWAHCTLCAPPGMXXXXXXXXXXXXXRLRISASMFTXXXXXXXXXXXXXX 528
F+H + YCWAHCTLCAPPG+ L S+S+ T
Sbjct: 808 FHHHHFF*FYCWAHCTLCAPPGISSSSSYASCCCCCFLLFSSSILTS*SVVSSHSSISMS 629
Query: 529 VYCDTGLGGSTASRALHCSGLSESGNSTEKWMYSLPFMNGLWYMGMPSSLIALKESGFTT 708
V CD GL G TA + S L SGNST K M SLPF+NGLWY+G+PSSLIAL ESG TT
Sbjct: 628 VNCDEGLAGRTACKPFT*SRLRVSGNSTVK*M*SLPFINGLWYIGIPSSLIALYESGLTT 449
Query: 709 SPGFDLISSCLLSK*VTTNWKPHRDSVKVRVCS*KRSSPFLLNLGCSFCWRTKMISPVTV 888
SPGFDLI S SK*V+TNWKPH+ SV+ VCS KRSS FLLNLGCSFC T ISPVTV
Sbjct: 448 SPGFDLIRSWRPSK*VSTNWKPHKASVRDSVCSTKRSSLFLLNLGCSFC*STNTISPVTV 269
Query: 889 SGASSASPGNVIFCPFCMPFSTNTSRTFFSCTTLSPLHCGHLSLSLIVSPEPLHSLQGCC 1068
SGASSASPGNVIF FC+PFST TS TFFSCTT SPLHCG SLSLIVSP PLHSLQGCC
Sbjct: 268 SGASSASPGNVIF*LFCIPFST*TSSTFFSCTTFSPLHCGQRSLSLIVSPVPLHSLQGCC 89
Query: 1069 ICWIIPGPNCLIWTLKPEPWQPAHLDEAP 1155
ICWII GP CLI T P P QPAH EAP
Sbjct: 88 ICWIIDGPRCLIETFIPVP*QPAHFMEAP 2
Score = 144 bits (310), Expect(2) = 1e-040
Identities = 70/137 (51%), Positives = 88/137 (64%)
Frame = -2 / +2
Query: 1011 MPTVQG**SCAGEEGS*SVCGERHAEWAEDHIPW*S**SA*HCHWRYHLCSPAEGASQVQ 832
+ TVQG C EE +*S C ER+AE +ED+IPW*S *S H +W Y +C+ AE QVQ
Sbjct: 146 LSTVQGREGCTREEST*SSCRERNAEQSEDYIPW*SR*STRHSYWGYCICASAERTPQVQ 325
Query: 831 EKG**PLLRAHPDLD*ISVWLPVCCDSLG*QAAADQIKSGRSCEA*FFQGDQRRRHAHVP 652
+K * P RAH * VW P+C SLG + QI+ RSC+A*F QG R +A VP
Sbjct: 326 KKE*RPFCRAHTVSY*GFVWFPICAYSLGWSPTSYQIEPRRSCQA*FIQGY*R*GNADVP 505
Query: 651 EAVHEGQAVHPLLGGVP 601
EA++EGQA+H L G+P
Sbjct: 506 EAIYEGQALHSLHCGIP 556
Score = 130 bits (278), Expect(3) = 3e-042
Identities = 66/154 (42%), Positives = 94/154 (61%)
Frame = +3 / -1
Query: 474 FDVHVIGHRLLALVQLHVGVLRHRLGREHSLQGLALLRAQRVRELHREVDVQLALHERPL 653
F + ++ LAL+ LHV L R ++SLQ L++ V E H EV+V+LALH+ PL
Sbjct: 683 FHIDIMKCSFLALIDLHVCQL**RFSWKNSLQTFHLIKT*SVGEFHSEVNVKLALHKWPL 504
Query: 654 VHGHAFVVDRLERIRLHNFARI*FDQQLPVIQVSHNKLEATQRFSQGQGVLVEEVITXXX 833
VH H+ +V+ L IRL NF+ + FD++L IQVS +KLE TQ S+ Q VL ++V T
Sbjct: 503 VHRHSLIVNSLV*IRLDNFSGVRFDKKLATIQVSKHKLETTQSLSKRQCVLYKKVFTLSF 324
Query: 834 XXXXXXXXENKDDISSDSVRRFISFTRECDLLPI 935
++K +I S+ V FI FTREC+LL +
Sbjct: 323 ELGVFFLLKHKYNIPSNCVWCFIGFTRECNLLTV 222
Score = 46.9 bits (96), Expect(3) = 3e-042
Identities = 16/31 (51%), Positives = 23/31 (74%)
Frame = +3 / -1
Query: 990 ITLALWASILVADCLSRTLALVARMLHLLDH 1082
++LALW ++ + D + TL +ARMLHLLDH
Sbjct: 167 LSLALWTAVFIIDSFTGTLTFIARMLHLLDH 75
Score = 37.7 bits (76), Expect(2) = 1e-040
Identities = 22/48 (45%), Positives = 27/48 (56%)
Frame = -1 / +1
Query: 1138 VLVAKVLALRSKSGSWGLE*SSKCSILATSARVLERQSATRIDAHSAR 995
VLV K L R + +*SSKCSILA + RV + S + HSAR
Sbjct: 19 VLVVKELV*RFLLDTLAHQ*SSKCSILAMNVRVPVKLSMIKTAVHSAR 162
Score = 29.0 bits (57), Expect(3) = 3e-042
Identities = 18/31 (58%), Positives = 18/31 (58%)
Frame = +2 / -3
Query: 914 GM*SSAHSACLSPQTLQEPSSPAQLYHPCTV 1006
GM*SS SA LS LQ SS Q PCTV
Sbjct: 243 GM*SSDCSAFLSRHELQVLSSLVQPSLPCTV 151
>gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein MsJ1 gene, complete cds
Length = 4449
Score = 128 bits (274), Expect(8) = e-148
Identities = 49/66 (74%), Positives = 58/66 (87%)
Frame = -3 / +3
Query: 893 PDTVTGDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTESLCGFQFVVTHLDNRQLLIKSNP 714
PDT+TGDI+FVLQ K HPKF+R+ DDL EH L+LT++LCGFQF VTHLD RQLL+KSNP
Sbjct: 2913 PDTITGDIVFVLQVKGHPKFRRERDDLHIEHNLSLTDALCGFQFNVTHLDGRQLLVKSNP 3092
Query: 713 GEVVKP 696
GEV+KP
Sbjct: 3093 GEVIKP 3110
Score = 96.8 bits (205), Expect(8) = e-148
Identities = 36/44 (81%), Positives = 43/44 (97%)
Frame = -3 / +1
Query: 1610 KKSDNTRYYEILGVSKDASQDDLKKAYRKAAIKNHPDKGGDPEK 1479
+KSDNT+YY+ILGVSK AS+D++KKAYRKAA+KNHPDKGGDPEK
Sbjct: 79 RKSDNTKYYDILGVSKSASEDEIKKAYRKAAMKNHPDKGGDPEK 210
Score = 92.3 bits (195), Expect(7) = 1e-074
Identities = 40/66 (60%), Positives = 47/66 (71%)
Frame = +1 / -3
Query: 697 GFTTSPGFDLISSCLLSK*VTTNWKPHRDSVKVRVCS*KRSSPFLLNLGCSFCWRTKMIS 876
G TSPGFDL +SCL S+ VT NWKP R SVK+++CS RSS L N GC F +TK +S
Sbjct: 3109 GLMTSPGFDLTNSCLPSRCVTLNWKPQRASVKLKLCSM*RSSRSLRNFGCPFTCKTKTMS 2930
Query: 877 PVTVSG 894
PV VSG
Sbjct: 2929 PVIVSG 2912
Score = 91.8 bits (194), Expect(8) = e-148
Identities = 35/48 (72%), Positives = 41/48 (85%)
Frame = -3 / +2
Query: 1040 SGETISDKDRCPQCKGDKVVQEKKVLEVFVEKGMQNGQKITFPGEADE 897
+GE IS++DRCPQCKG+K+ QEKKVLEV VEKGMQ G KI F G+ADE
Sbjct: 2609 AGEVISERDRCPQCKGNKITQEKKVLEVHVEKGMQQGHKIVFEGQADE 2752
Score = 81.7 bits (172), Expect(8) = e-148
Identities = 30/45 (66%), Positives = 36/45 (80%)
Frame = -3 / +3
Query: 1169 KGSKSGASSRCAGCQGSGFKVQIRQLGPGMIQQMQHPCNECKGSG 1035
KGSKSG + RC GCQG+G K+ RQ+G GMIQQMQH C +CKG+G
Sbjct: 2400 KGSKSGTAGRCFGCQGTGMKITRRQIGLGMIQQMQHVCPDCKGTG 2534
Score = 76.2 bits (160), Expect(8) = e-148
Identities = 30/59 (50%), Positives = 41/59 (69%)
Frame = -3 / +2
Query: 593 LSPEQCKALEAVLPPKPVSQYTDMELDECEETMPYDVNIEAEMRRRQQQHQEAYDEDED 417
LSP Q LE +LP K + E+D+CEET +DVNI EM R++QQ++EAYD+D+D
Sbjct: 3302 LSPSQSLELEKILPQKTSKNLSQKEVDDCEETTLHDVNIAEEMSRKKQQYREAYDDDDD 3478
Score = 72.5 bits (152), Expect(8) = e-148
Identities = 28/37 (75%), Positives = 33/37 (89%)
Frame = -3 / +1
Query: 1274 DDVVHPLKVSLEDLYNGTSKKLSLSRSVLCSKCNGKG 1164
+DVVH +KVSLED+YNGT+KKLSLSR+ LCSKC G G
Sbjct: 1291 EDVVHSIKVSLEDVYNGTTKKLSLSRNALCSKCKGYG 1401
Score = 72.1 bits (151), Expect(7) = 1e-074
Identities = 34/45 (75%), Positives = 35/45 (77%)
Frame = +1 / -2
Query: 1474 LNFSGSPPLSGWFLMAALR*AFLRSSCDASLETPRIS*YRVLSLF 1608
L FSGSPPLSGWF +AA *AFL SS A ETPRIS*Y VLSLF
Sbjct: 215 LTFSGSPPLSGWFFIAAFL*AFLISSSLALFETPRIS*YLVLSLF 81
Score = 69.8 bits (146), Expect(7) = 1e-074
Identities = 31/48 (64%), Positives = 34/48 (70%)
Frame = +1 / -1
Query: 895 ASSASPGNVIFCPFCMPFSTNTSRTFFSCTTLSPLHCGHLSLSLIVSP 1038
+SSA P N I P C+PFST TS TFFS L PLH GHLSLSL+ SP
Sbjct: 2754 SSSACPSNTIL*PCCIPFSTCTSSTFFS*VILFPLH*GHLSLSLMTSP 2611
Score = 68.0 bits (142), Expect(8) = e-148
Identities = 26/31 (83%), Positives = 29/31 (93%)
Frame = -3 / +1
Query: 1481 KFKELAQGYEVLSDPEKREIYDQYGEDALKE 1389
+FKEL Q YEVLSDPEK+E+YDQYGEDALKE
Sbjct: 979 QFKELGQAYEVLSDPEKKELYDQYGEDALKE 1071
Score = 65.7 bits (137), Expect(8) = e-148
Identities = 25/31 (80%), Positives = 28/31 (90%)
Frame = -3 / +2
Query: 686 KAINDEGMPMYQRPFMKGKLYIHFSVEFPDS 594
KAINDEGMP + RPFMKG+LYI FSV+FPDS
Sbjct: 3203 KAINDEGMPQHGRPFMKGRLYIKFSVDFPDS 3295
Score = 65.2 bits (136), Expect(7) = 1e-074
Identities = 26/53 (49%), Positives = 31/53 (58%)
Frame = +1 / -3
Query: 1036 PEPLHSLQGCCICWIIPGPNCLIWTLKPEPWQPAHLDEAPDFEPLPLHLEQRT 1194
P PL S Q CCIC I+P P CL+ P PW P HL P FEP ++ + RT
Sbjct: 2533 PVPLQSGQTCCIC*IMPSPICLLVIFIPVPWHPKHLPAVPLFEPFLINQKFRT 2375
Score = 52.8 bits (109), Expect(5) = 3e-022
Identities = 23/41 (56%), Positives = 26/41 (63%)
Frame = +1 / -2
Query: 1165 PLPLHLEQRTLRDRESFFDVPLYKSSRETFRG*TTSSPRRC 1287
P PLH E + +SFF VPLY SS+ETF TTSSP C
Sbjct: 1400 PYPLHFEHSAFLESDSFFVVPLYTSSKETFIECTTSSPCFC 1278
Score = 43.7 bits (89), Expect(7) = 1e-074
Identities = 20/31 (64%), Positives = 21/31 (67%)
Frame = +1 / -1
Query: 595 ESGNSTEKWMYSLPFMNGLWYMGMPSSLIAL 687
ESG ST MY PFMNGL G+PSS IAL
Sbjct: 3294 ESGKSTLNLMYRRPFMNGLPCCGIPSSFIAL 3202
Score = 41.8 bits (85), Expect(5) = 3e-022
Identities = 24/65 (36%), Positives = 35/65 (53%)
Frame = +3 / -2
Query: 699 LHNFARI*FDQQLPVIQVSHNKLEATQRFSQGQGVLVEEVITXXXXXXXXXXXENKDDIS 878
L +FA + FDQ LP I++ KLEAT+ S Q V +VIT ++K+ +S
Sbjct: 3107 LDDFAGVRFDQ*LPSIKMCDIKLEATESIS*AQIVFNVKVITLPPKLRMSLYLQDKNYVS 2928
Query: 879 SDSVR 893
D +R
Sbjct: 2927 CDCIR 2913
Score = 37.3 bits (75), Expect(7) = 1e-074
Identities = 20/30 (66%), Positives = 21/30 (70%)
Frame = +1 / -2
Query: 1390 SLRASSPYWS*ISLFSGSLRTS*P*ASSLN 1479
SLRASSPY S S FSGSL TS +SLN
Sbjct: 1070 SLRASSPY*SYSSFFSGSLNTSYAWPNSLN 981
Score = 36.8 bits (74), Expect(5) = 3e-022
Identities = 14/37 (37%), Positives = 25/37 (67%)
Frame = +3 / -3
Query: 567 QGLALLRAQRVRELHREVDVQLALHERPLVHGHAFVV 677
Q LA + R+RE++ ++DV +LHERP + H+ ++
Sbjct: 3322 QALAWGKKTRIREINTKLDV*ASLHERPTMLWHSLII 3212
Score = 35.9 bits (72), Expect(5) = 3e-022
Identities = 14/28 (50%), Positives = 19/28 (67%)
Frame = +3 / -1
Query: 1383 HSLLEGILTILVIDLSLFRVAEDLITLS 1466
HS +GI TIL+I LFR+A+ I L+
Sbjct: 1077 HSFFKGIFTILIIQFFLFRIAQHFICLA 994
Score = 31.3 bits (62), Expect(7) = 1e-074
Identities = 14/27 (51%), Positives = 16/27 (59%)
Frame = +2 / -3
Query: 1211 ASLMCHCTNPPEKPSEDEPHHLPAVAF 1291
ASL C T+PP KP + PH L A F
Sbjct: 1354 ASL*CRYTHPPRKPL*NAPHLLLASVF 1274
Score = 23.1 bits (44), Expect(5) = 3e-022
Identities = 9/24 (37%), Positives = 16/24 (66%)
Frame = +3 / -2
Query: 1041 TLALVARMLHLLDHSRPQLPDLDL 1112
+ A+ +LHLL+H++ LP +L
Sbjct: 2528 SFAVRTDVLHLLNHAQSNLPSGNL 2457
>gb|CO513103.1|CO513103 s13dSG89H0300028_122132 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 550
Score = 182 bits (392), Expect(3) = 5e-070
Identities = 74/81 (91%), Positives = 78/81 (96%)
Frame = -3 / +1
Query: 1631 EMFGRAPKKSDNTRYYEILGVSKDASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQGYE 1452
+MFGRAPKKSD+TRYYEILGVSK ASQDDLKKAY+KAAIKNHPDKGGDPEKFKELAQ YE
Sbjct: 79 KMFGRAPKKSDSTRYYEILGVSKTASQDDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYE 258
Query: 1451 VLSDPEKREIYDQYGEDALKE 1389
VLSDPEKR+IYD YGEDALKE
Sbjct: 259 VLSDPEKRDIYDTYGEDALKE 321
Score = 126 bits (269), Expect(3) = 2e-047
Identities = 62/80 (77%), Positives = 68/80 (85%)
Frame = +1 / -3
Query: 1390 SLRASSPYWS*ISLFSGSLRTS*P*ASSLNFSGSPPLSGWFLMAALR*AFLRSSCDASLE 1569
SLRASSPY S*IS FSGSL+TS* ASSLNFSGSPPLSG FL+AA *AF +SS +A L+
Sbjct: 320 SLRASSPYVS*ISRFSGSLKTS*ACASSLNFSGSPPLSG*FLIAAFL*AFFKSS*EAVLD 141
Query: 1570 TPRIS*YRVLSLFFGARPNI 1629
TP+IS*Y VLSLFFGA PNI
Sbjct: 140 TPKIS*YLVLSLFFGALPNI 81
Score = 87.2 bits (184), Expect(3) = 5e-070
Identities = 36/40 (90%), Positives = 39/40 (97%)
Frame = -3 / +1
Query: 1274 DDVVHPLKVSLEDLYNGTSKKLSLSRSVLCSKCNGKGSKS 1155
+DVVHPLKVSLEDLY GTSKKLSLSR+VLCSKC+GKGSKS
Sbjct: 430 EDVVHPLKVSLEDLYLGTSKKLSLSRNVLCSKCSGKGSKS 549
Score = 73.9 bits (155), Expect(3) = 2e-047
Identities = 30/44 (68%), Positives = 34/44 (77%)
Frame = +1 / -3
Query: 1156 DFEPLPLHLEQRTLRDRESFFDVPLYKSSRETFRG*TTSSPRRC 1287
D EPLPLH E +T +RESF DVP +SS+ET RG*TTSSPR C
Sbjct: 548 DLEPLPLHFEHKTFLERESFLDVPR*RSSKETLRG*TTSSPRLC 417
Score = 29.9 bits (59), Expect(3) = 5e-070
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = -2 / +3
Query: 1377 RWWDA*SL*HIPVILWWW 1324
R W * +*H+ V LWWW
Sbjct: 336 RRWWP*PI*HLLVFLWWW 389
Score = 23.5 bits (45), Expect(3) = 2e-047
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = +2 / -1
Query: 1325 HHQRMTGICQRDH 1363
HHQR T CQ H
Sbjct: 388 HHQRKTRRCQMGH 350
>emb|Z71997.1|MSDNAJLP M.sativa mRNA for DnaJ-like protein
Length = 513
Score = 206 bits (445), Expect(2) = 8e-061
Identities = 79/110 (71%), Positives = 95/110 (86%)
Frame = -3 / +3
Query: 1274 DDVVHPLKVSLEDLYNGTSKKLSLSRSVLCSKCNGKGSKSGASSRCAGCQGSGFKVQIRQ 1095
+DVVH +KVSLED+YNGT+KKLSLSR+ LCSKC GKGSKSG + RC GCQG+G K+ RQ
Sbjct: 177 EDVVHSIKVSLEDVYNGTTKKLSLSRNALCSKCKGKGSKSGTAGRCFGCQGTGMKITRRQ 356
Query: 1094 LGPGMIQQMQHPCNECKGSGETISDKDRCPQCKGDKVVQEKKVLEVFVEK 945
+G GMIQQMQH C +CKG+GE IS++DRCPQCKG+K+ Q+KKVLEV VEK
Sbjct: 357 IGLGMIQQMQHVCPDCKGTGEVISERDRCPQCKGNKITQQKKVLEVHVEK 506
Score = 149 bits (320), Expect(2) = 5e-040
Identities = 66/114 (57%), Positives = 72/114 (63%)
Frame = +1 / -3
Query: 946 FSTNTSRTFFSCTTLSPLHCGHLSLSLIVSPEPLHSLQGCCICWIIPGPNCLIWTLKPEP 1125
FST TS TFF L PLH GHLSLSL+ SP PL S Q CCIC I+P P CL+ P P
Sbjct: 505 FSTCTSSTFFC*VILFPLH*GHLSLSLMTSPVPLQSGQTCCIC*IMPSPICLLVIFIPVP 326
Query: 1126 WQPAHLDEAPDFEPLPLHLEQRTLRDRESFFDVPLYKSSRETFRG*TTSSPRRC 1287
W P HL P FEP PLH E + +SFF VPLY SS+ETF TTSSP C
Sbjct: 325 WHPKHLPAVPLFEPFPLHFEHSAFLESDSFFVVPLYTSSKETFIECTTSSPCFC 164
Score = 43.2 bits (88), Expect(2) = 8e-061
Identities = 16/18 (88%), Positives = 17/18 (94%)
Frame = -3 / +3
Query: 1442 DPEKREIYDQYGEDALKE 1389
DPEK+E YDQYGEDALKE
Sbjct: 6 DPEKKESYDQYGEDALKE 59
Score = 30.9 bits (61), Expect(2) = 5e-040
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = +3 / -2
Query: 1383 HSLLEGILTILVIDLSLFRVA 1445
HS +GI TIL+I LFR+A
Sbjct: 65 HSFFKGIFTILIIRFFLFRIA 3
>gb|AW698418.1|AW698418 G266 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 273
Score = 134 bits (288), Expect = 2e-032
Identities = 54/80 (67%), Positives = 66/80 (82%)
Frame = -3 / -2
Query: 656 YQRPFMKGKLYIHFSVEFPDSLSPEQCKALEAVLPPKPVSQYTDMELDECEETMPYDVNI 477
YQRPFMKGKLYIHF+VEFPD+LS +Q K L+AVLP KP SQ TDME+DECEET +DVN+
Sbjct: 266 YQRPFMKGKLYIHFTVEFPDTLSLDQVKGLQAVLPAKPSSQLTDMEIDECEETTLHDVNM 87
Query: 476 EAEMRRRQQQHQEAYDEDED 417
E E RR+QQQ Q+ +++D
Sbjct: 86 EEENRRKQQQQQQEAYDEDD 27
>gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 316
Score = 123 bits (263), Expect = 7e-029
Identities = 50/81 (61%), Positives = 64/81 (79%)
Frame = -3 / -1
Query: 659 MYQRPFMKGKLYIHFSVEFPDSLSPEQCKALEAVLPPKPVSQYTDMELDECEETMPYDVN 480
+YQRPFMKGKLYIHF+VEF D+LS +Q K L+ V+P KP SQ TDME+DECEET +DVN
Sbjct: 289 VYQRPFMKGKLYIHFTVEFTDTLSLDQVKGLQDVIPGKPSSQLTDMEIDECEETTLHDVN 110
Query: 479 IEAEMRRRQQQHQEAYDEDED 417
+E E R +QQQ Q+ +++D
Sbjct: 109 MEEENRSKQQQQQQ*AYDEDD 47
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,681,263
Number of Sequences: 7669
Number of extensions: 203585
Number of successful extensions: 2925
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 469232980
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)