TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192958.2.1
(1629 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like pr... 203 e-148
gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x va... 352 e-138
gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein Ms... 125 e-126
gb|CO513103.1|CO513103 s13dSG89H0300028_122132 Glandular tr... 184 1e-067
emb|Z71997.1|MSDNAJLP M.sativa mRNA for DnaJ-like protein 182 1e-053
gb|AW698418.1|AW698418 G266 glandular-haired subtracted cDN... 138 2e-033
gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDN... 127 4e-030
>emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like protein
Length = 1545
Score = 203 bits (438), Expect(3) = e-148
Identities = 81/126 (64%), Positives = 95/126 (75%)
Frame = -1 / +1
Query: 1269 EDVVHPLKVSLEDLYNGTSKKLSLSRSVLCSKCNGKGSKSGASSRCAGCQGSGFKVQIRQ 1090
EDVVH +KVSLED+YNGT+KKLSLSR+ LCSKC GKGSKSG + RC GCQG+G K+ RQ
Sbjct: 418 EDVVHSIKVSLEDVYNGTTKKLSLSRNALCSKCKGKGSKSGTAGRCFGCQGTGMKITRRQ 597
Query: 1089 LGPGMIQQMQHPCNECKGSGETISDKDRCPQCKGDXXXXXXXXXXXXXXKGMQNGQKITF 910
+G GMIQQMQH C +CKG+GE IS++DRCPQCKG+ KGMQ G KI F
Sbjct: 598 IGLGMIQQMQHVCPDCKGTGEVISERDRCPQCKGNKITQEKKVLEVHVEKGMQQGHKIVF 777
Query: 909 PGEADE 892
G+ADE
Sbjct: 778 EGQADE 795
Score = 194 bits (419), Expect(3) = e-148
Identities = 78/116 (67%), Positives = 94/116 (81%)
Frame = -1 / +1
Query: 870 EADEAPDTTTGDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTESLCGFQFVLTHLDNRQLL 691
+ADE PDT TGDI+FVLQ K HPKF+R+ DDL EH L+LT++LCGFQF +THLD RQLL
Sbjct: 784 QADELPDTITGDIVFVLQVKGHPKFRRERDDLHIEHNLSLTDALCGFQFNVTHLDGRQLL 963
Query: 690 IKSNPGEVVKPDSFKAINDEGMPMYQRPFMKGKLYIHFSVEFPDSLSPEQCKALEV 523
+KSNPGEV+KP KAINDEGMP + RPFMKG+LYI FSV+FPDS ++LE+
Sbjct: 964 VKSNPGEVIKPGQHKAINDEGMPQHGRPFMKGRLYIKFSVDFPDSGFLSPSQSLEL 1131
Score = 163 bits (350), Expect(3) = e-148
Identities = 63/74 (85%), Positives = 72/74 (97%)
Frame = -1 / +1
Query: 1608 KKSDNTRYYEILGVSKDASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEK 1429
+KSDNT+YY+ILGVSK AS+D++KKAYRKAA+KNHPDKGGDPEKFKEL QAYEVLSDPEK
Sbjct: 79 RKSDNTKYYDILGVSKSASEDEIKKAYRKAAMKNHPDKGGDPEKFKELGQAYEVLSDPEK 258
Query: 1428 REIYDQYGEDALKE 1387
+E+YDQYGEDALKE
Sbjct: 259 KELYDQYGEDALKE 300
Score = 127 bits (271), Expect(3) = 8e-065
Identities = 61/127 (48%), Positives = 66/127 (51%)
Frame = +2 / -2
Query: 890 ASSASPGNVIFCPFCIPXXXXXXXXXXXXXXLSPLHCGHLSLSLMVSPEPLHSLQGXXXX 1069
+SSA P N I P CIP L PLH GHLSLSLM SP PL S Q
Sbjct: 797 SSSACPSNTIL*PCCIPFSTCTSSTFFS*VILFPLH*GHLSLSLMTSPVPLQSGQTCCIC 618
Query: 1070 XXXPGPNCRIWTLKPEPWQPAHLDEAPDFEPLPLHLEQRTLRDRESFFDVPLYKSSRETF 1249
P P C + P PW P HL P FEP PLH E + +SFF VPLY SS+ETF
Sbjct: 617 *IMPSPICLLVIFIPVPWHPKHLPAVPLFEPFPLHFEHSAFLESDSFFVVPLYTSSKETF 438
Query: 1250 RG*TTSS 1270
TTSS
Sbjct: 437 IECTTSS 417
Score = 83.1 bits (175), Expect(3) = 8e-065
Identities = 43/73 (58%), Positives = 44/73 (60%)
Frame = +2 / -2
Query: 1388 SLRAXXXXXXXXXXXXGSLRTXXXXXXXLNFSGSPPLSGWFLMAALR*AFLRSSWDASLE 1567
SLRA GSL T LNFSGSPPLSGWF +AA *AFL SS A E
Sbjct: 299 SLRASSPY*SYSSFFSGSLNTSYAWPNSLNFSGSPPLSGWFFIAAFL*AFLISSSLALFE 120
Query: 1568 TPRIS*YRVLSLF 1606
TPRIS*Y VLSLF
Sbjct: 119 TPRIS*YLVLSLF 81
Score = 79.9 bits (168), Expect(3) = 8e-065
Identities = 37/62 (59%), Positives = 40/62 (64%)
Frame = +2 / -2
Query: 557 ESGNSTEK*MYSLPFMKGL*YMGMPSSLIALKESGFTTSPGFDLISSCLLSK*VRTNWKP 736
ESG ST MY PFM GL G+PSS IAL G TSPGFDL +SCL S+ V NWKP
Sbjct: 1097 ESGKSTLNLMYRRPFMNGLPCCGIPSSFIALC*PGLMTSPGFDLTNSCLPSRCVTLNWKP 918
Query: 737 HR 742
R
Sbjct: 917 QR 912
Score = 79.0 bits (166), Expect(4) = 1e-042
Identities = 31/59 (52%), Positives = 41/59 (69%)
Frame = -1 / +1
Query: 555 LSPEQCKALEVVLPPKPVSQYTDMELDECEETMTYDVNIEEEMRRRQQHHQEAYDEDDD 379
LSP Q LE +LP K + E+D+CEET +DVNI EEM R++Q ++EAYD+DDD
Sbjct: 1105 LSPSQSLELEKILPQKTSKNLSQKEVDDCEETTLHDVNIAEEMSRKKQQYREAYDDDDD 1281
Score = 71.6 bits (150), Expect(5) = 3e-038
Identities = 41/115 (35%), Positives = 61/115 (53%)
Frame = +1 / -1
Query: 529 QGLALLRAQRVRELHRKVNVQLALHEGPLIHGHAFIVDCLERIRLHNFTRVRFDQQLPVI 708
Q LA + R+RE++ K++V +LHE P + H+ I+ L +F VRFDQ LP I
Sbjct: 1125 QALAWGKKTRIREINTKLDV*ASLHERPTMLWHSLIIYSFMLTWLDDFAGVRFDQ*LPSI 946
Query: 709 QVSQNKLEATQRFSQGQCVLVEEVIAXXXXXXXXXXXENKDDISSGSIRCFISFA 873
++ KLEAT+ S Q V +VI ++K+ +S IR FIS +
Sbjct: 945 KMCDIKLEATESIS*AQIVFNVKVITLPPKLRMSLYLQDKNYVSCDCIREFISLS 781
Score = 66.1 bits (138), Expect(4) = 1e-042
Identities = 36/93 (38%), Positives = 49/93 (52%)
Frame = -2 / +2
Query: 1268 RMWFIL*RFLWRICTMAHQRSSLCPAVFSAPSAMVRVQSLELHRGVLVAKVLALRSKSGS 1089
+MW IL*RF WR+C A QRS + A + +VQ +EL GVL A+ R G
Sbjct: 419 KMWCIL*RFPWRMCITALQRSYHFLGMHCAQNVKGKVQKVELLEGVLDARAQV*RLPEGK 598
Query: 1088 WGLE*FSKCSILAMSARVLERPSAIRIDAHNAK 990
*F+KC+ ++A+ R IDA NA+
Sbjct: 599 LDWA*FNKCNTSVLTAKEQARSLVREIDALNAR 697
Score = 58.3 bits (121), Expect(5) = 3e-038
Identities = 26/72 (36%), Positives = 44/72 (61%)
Frame = +1 / -1
Query: 892 FISFARECDLLPVLHTFLNYHFKNFLLRHNFITFALWASILIADGLSRTLALIARMLHLL 1071
FIS + E +L+ +LH + H ++ L N ++ AL ASI + + L+ + A+ +LHLL
Sbjct: 795 FISLSFEYNLVTLLHPLFHMHLQHLLFLSNLVSLALRASISLTNDLACSFAVRTDVLHLL 616
Query: 1072 NHSRPQLPDLDL 1107
NH++ LP +L
Sbjct: 615 NHAQSNLPSGNL 580
Score = 49.2 bits (101), Expect(4) = 1e-042
Identities = 27/54 (50%), Positives = 32/54 (59%)
Frame = -3 / +3
Query: 1540 QEGLPQGRHQEPPRQGRRSREVQGASSGL*GPQ*S*KARDL*SIW*GCPQGGNG 1379
QE L +G +EP R G S EVQG +* + S*K R +*SIW* CP NG
Sbjct: 147 QESL*KGSDEEPSR*GWGS*EVQGVGPSI*SVERS*KERTV*SIW*RCP*RRNG 308
Score = 41.4 bits (84), Expect(4) = 1e-042
Identities = 23/59 (38%), Positives = 32/59 (54%)
Frame = -3 / +3
Query: 853 *YYHWRYHLCSPAEGASQVQEKGR*PLLRAHTDLD*ISVWLPVCSDSLG*QAAADQIEP 677
*Y H R+ C ++G S+V E *P +L+* S+WLPV + +A QIEP
Sbjct: 801 *YNHRRHSFCLASKGTSEVSEGA**PSH*TQFELN*CSLWLPV*CHTS*WKATIGQIEP 977
Score = 39.1 bits (79), Expect(5) = 3e-038
Identities = 24/64 (37%), Positives = 29/64 (45%)
Frame = +3 / -3
Query: 1122 GNQHTSMKLQTLNPYHCTWSREHCGTERASLMCHCTNPPEKPLEDEPHPLPFAAFCPYCC 1301
G Q+T + LN S H ASL C T+PP KPL + PH L + F
Sbjct: 565 GIQNTFQQFHFLNLSLYILSTVHS*KVIASL*CRYTHPPRKPL*NAPHLLLASVFLRVKD 386
Query: 1302 HHLQ 1313
HH Q
Sbjct: 385 HHHQ 374
Score = 31.8 bits (63), Expect(5) = 3e-038
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +1 / -1
Query: 1381 HSLLEGILTILIINLSLFRITE 1446
HS +GI TILII LFRI +
Sbjct: 306 HSFFKGIFTILIIQFFLFRIAQ 241
Score = 26.3 bits (51), Expect(5) = 3e-038
Identities = 18/41 (43%), Positives = 20/41 (48%)
Frame = +3 / -3
Query: 1425 LAFQDH*GPHKPELAP*TSRDRRPCRGGS*WRPCGRPS*GH 1547
L+FQD H P*TS+D P GS P R S* H
Sbjct: 262 LSFQDRSTLHMLGPTP*TSQDPHPYLDGSSSLPFYRLS*FH 140
>gb|AJ248336.1|AJ248336 AJ248336 Medicago sativa subsp. x varia young root nodule Medicago
sativa subsp. x varia cDNA clone MsNod658, mRNA sequence
Length = 1004
Score = 352 bits (762), Expect(2) = e-138
Identities = 141/172 (81%), Positives = 155/172 (90%)
Frame = -1 / +3
Query: 870 EADEAPDTTTGDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTESLCGFQFVLTHLDNRQLL 691
EADEAPDT TGDI+FVLQQKEHPKFKRK +DLF EHTL+LTE+LCGFQFVLTHLD RQLL
Sbjct: 249 EADEAPDTVTGDIVFVLQQKEHPKFKRKSEDLFVEHTLSLTEALCGFQFVLTHLDGRQLL 428
Query: 690 IKSNPGEVVKPDSFKAINDEGMPMYQRPFMKGKLYIHFSVEFPDSLSPEQCKALEVVLPP 511
IKSNPGEVVKPDS+KAINDEGMPMYQRPFMKGKLYIHF+VEFPD+LS +Q K L+ VLP
Sbjct: 429 IKSNPGEVVKPDSYKAINDEGMPMYQRPFMKGKLYIHFTVEFPDTLSLDQVKGLQAVLPA 608
Query: 510 KPVSQYTDMELDECEETMTYDVNIEEEMRRRQQHHQEAYDEDDDVPGGGQRV 355
KP SQ TDME+DECEET +DVN+EEE RR+QQ QEAYDEDDD+PGG QRV
Sbjct: 609 KPSSQLTDMEIDECEETTLHDVNMEEENRRKQQQQQEAYDEDDDMPGGAQRV 764
Score = 197 bits (425), Expect(2) = 1e-076
Identities = 89/168 (52%), Positives = 95/168 (56%)
Frame = +2 / -2
Query: 338 YCWAHCTLCPPPGTXXXXXXXXWWCCCRLRISSSMFTXXXXXXXXXXXXXXXYCDTGLGG 517
YCWAHCTLC PPG CCC L SSS+ T CD GL G
Sbjct: 781 YCWAHCTLCAPPGISSSSSYASCCCCCFLLFSSSILTS*SVVSSHSSISMSVNCDEGLAG 602
Query: 518 RTTSRALHCSGLNESGNSTEK*MYSLPFMKGL*YMGMPSSLIALKESGFTTSPGFDLISS 697
RT + S L SGNST K*M SLPF+ GL Y+G+PSSLIAL ESG TTSPGFDLI S
Sbjct: 601 RTACKPFT*SRLRVSGNSTVK*M*SLPFINGLWYIGIPSSLIALYESGLTTSPGFDLIRS 422
Query: 698 CLLSK*VRTNWKPHRDXXXXXXXXXXXXXPFLLNLGCSFCWRTKMISP 841
SK*V TNWKPH+ FLLNLGCSFC T ISP
Sbjct: 421 WRPSK*VSTNWKPHKASVRDSVCSTKRSSLFLLNLGCSFC*STNTISP 278
Score = 156 bits (336), Expect(2) = e-138
Identities = 62/87 (71%), Positives = 67/87 (77%)
Frame = -1 / +3
Query: 1149 GASSRCAGCQGSGFKVQIRQLGPGMIQQMQHPCNECKGSGETISDKDRCPQCKGDXXXXX 970
GAS +CAGCQG+G KV IR LGP MIQQMQHPCNECKG+GETI+DKDRCPQCKG+
Sbjct: 3 GASMKCAGCQGTGMKVSIRHLGPSMIQQMQHPCNECKGTGETINDKDRCPQCKGEKVVQE 182
Query: 969 XXXXXXXXXKGMQNGQKITFPGEADEA 889
KGMQN QKITFPGEADEA
Sbjct: 183 KKVLEVHVEKGMQNSQKITFPGEADEA 263
Score = 116 bits (247), Expect(2) = 3e-049
Identities = 53/118 (44%), Positives = 75/118 (63%)
Frame = +1 / -1
Query: 517 ENNLQGLALLRAQRVRELHRKVNVQLALHEGPLIHGHAFIVDCLERIRLHNFTRVRFDQQ 696
+N+LQ L++ V E H +VNV+LALH+ PL+H H+ IV+ L IRL NF+ VRFD++
Sbjct: 602 KNSLQTFHLIKT*SVGEFHSEVNVKLALHKWPLVHRHSLIVNSLV*IRLDNFSGVRFDKK 423
Query: 697 LPVIQVSQNKLEATQRFSQGQCVLVEEVIAXXXXXXXXXXXENKDDISSGSIRCFISF 870
L IQVS++KLE TQ S+ QCVL ++V ++K +I S + CFI F
Sbjct: 422 LATIQVSKHKLETTQSLSKRQCVLYKKVFTLSFELGVFFLLKHKYNIPSNCVWCFIGF 249
Score = 105 bits (223), Expect(2) = 1e-076
Identities = 49/87 (56%), Positives = 50/87 (57%)
Frame = +2 / -2
Query: 890 ASSASPGNVIFCPFCIPXXXXXXXXXXXXXXLSPLHCGHLSLSLMVSPEPLHSLQGXXXX 1069
ASSASPGNVIF FCIP SPLHCG SLSL+VSP PLHSLQG
Sbjct: 262 ASSASPGNVIF*LFCIPFST*TSSTFFSCTTFSPLHCGQRSLSLIVSPVPLHSLQGCCIC 83
Query: 1070 XXXPGPNCRIWTLKPEPWQPAHLDEAP 1150
GP C I T P P QPAH EAP
Sbjct: 82 WIIDGPRCLIETFIPVP*QPAHFMEAP 2
Score = 101 bits (215), Expect(5) = 7e-038
Identities = 49/98 (50%), Positives = 63/98 (64%)
Frame = -3 / +2
Query: 856 T*YYHWRYHLCSPAEGASQVQEKGR*PLLRAHTDLD*ISVWLPVCSDSLG*QAAADQIEP 677
T + +W Y +C+ AE QVQ+K P RAHT * VW P+C+ SLG + QIEP
Sbjct: 263 TRHSYWGYCICASAERTPQVQKKE*RPFCRAHTVSY*GFVWFPICAYSLGWSPTSYQIEP 442
Query: 676 W*SCEA*FFQGNQR*RHAHVSEALHEGQAVHSLFGGVP 563
SC+A*F QG R* +A V EA++EGQA+HSL G+P
Sbjct: 443 RRSCQA*FIQGY*R*GNADVPEAIYEGQALHSLHCGIP 556
Score = 95.0 bits (201), Expect(2) = 3e-049
Identities = 36/64 (56%), Positives = 48/64 (75%)
Frame = +1 / -1
Query: 886 YCFISFARECDLLPVLHTFLNYHFKNFLLRHNFITFALWASILIADGLSRTLALIARMLH 1065
+CFI F REC+LL VLH+FL+ +FK FLL +N ++ ALW ++ I D + TL IARMLH
Sbjct: 266 WCFIGFTRECNLLTVLHSFLDMNFKYFLLLYNLLSLALWTAVFIIDSFTGTLTFIARMLH 87
Query: 1066 LLNH 1077
LL+H
Sbjct: 86 LLDH 75
Score = 84.0 bits (177), Expect(3) = 3e-025
Identities = 49/96 (51%), Positives = 57/96 (59%)
Frame = +3 / -3
Query: 582 ECTACPS*RASDTWACLHR*LP*KNQASQLHQGSI*SAAACYPSESEQTGSHTEIQSRSV 761
EC ACPS* AS T A HR* P NQA QL +GSI* +PSE Q G+HT+ Q +V
Sbjct: 537 ECKACPS*MASGTSAFPHR**PCMNQA*QLLRGSI**EVGDHPSE*AQIGNHTKPQ*ETV 358
Query: 762 CARRRGHRPFS*TWDAPSAGEQR*YLQW*YQVLHQL 869
CA ++G F *TW SA Q Y Q VLH+L
Sbjct: 357 CALQKGLHSFF*TWGVLSAEAQIQYPQ*LCLVLHRL 250
Score = 44.1 bits (90), Expect(5) = 7e-038
Identities = 25/58 (43%), Positives = 34/58 (58%)
Frame = -3 / +2
Query: 1078 NDSANAASLQ*VQGFWRDHQR*G*MPTMQR**SCAXXXXXXXXXXERYAERAEDHIPW 905
ND ANAASLQ*+ G+ ++Q * + T+Q C ER AE++ED+IPW
Sbjct: 74 NDPANAASLQ*M*GYR*NYQ**RPLSTVQGREGCTREEST*SSCRERNAEQSEDYIPW 247
Score = 36.8 bits (74), Expect(3) = 3e-025
Identities = 23/53 (43%), Positives = 24/53 (45%)
Frame = +3 / -3
Query: 993 CIVGIYPYR*WSLQNPCTHCKDAAFAESFQAPTAGFGP*SQNLGNQHTSMKLQ 1151
C V Y * + P HCKDAAFA S P Q L NQH S K Q
Sbjct: 159 CTVDSGLYH**FHRYPYIHCKDAAFAGSLMGQGV**KPSYQFLDNQHISWKPQ 1
Score = 29.0 bits (57), Expect(5) = 7e-038
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = -3 / +2
Query: 1150 WSFIEVCWLPRFWL*G 1103
W F E+CWL R W G
Sbjct: 2 WGFHEMCWLSRNWYEG 49
Score = 27.2 bits (53), Expect(3) = 3e-025
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = +3 / -3
Query: 885 LLLHQLRQGM*SSARSAY 938
L+LH+L QGM*SS SA+
Sbjct: 267 LVLHRLHQGM*SSDCSAF 214
Score = 25.8 bits (50), Expect(5) = 7e-038
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = -2 / +1
Query: 386 MMMCQAVGRGCSAPSSR 336
MM+C RG SAPSSR
Sbjct: 733 MMICLVGHRGYSAPSSR 783
Score = 25.4 bits (49), Expect(5) = 7e-038
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3 / +2
Query: 526 GCSPAQASITVH*HGARRVRGD 461
GCS + IT+ HG R VRG+
Sbjct: 593 GCSSS*TFITIDRHGDR*VRGN 658
>gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein MsJ1 gene, complete cds
Length = 4449
Score = 125 bits (267), Expect(7) = e-126
Identities = 48/66 (72%), Positives = 57/66 (86%)
Frame = -1 / +3
Query: 855 PDTTTGDIIFVLQQKEHPKFKRKGDDLFYEHTLTLTESLCGFQFVLTHLDNRQLLIKSNP 676
PDT TGDI+FVLQ K HPKF+R+ DDL EH L+LT++LCGFQF +THLD RQLL+KSNP
Sbjct: 2913 PDTITGDIVFVLQVKGHPKFRRERDDLHIEHNLSLTDALCGFQFNVTHLDGRQLLVKSNP 3092
Query: 675 GEVVKP 658
GEV+KP
Sbjct: 3093 GEVIKP 3110
Score = 97.3 bits (206), Expect(7) = e-126
Identities = 37/51 (72%), Positives = 45/51 (88%)
Frame = -1 / +1
Query: 1608 KKSDNTRYYEILGVSKDASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQA 1456
+KSDNT+YY+ILGVSK AS+D++KKAYRKAA+KNHPDKGGDPEK + A
Sbjct: 79 RKSDNTKYYDILGVSKSASEDEIKKAYRKAAMKNHPDKGGDPEKVSLMVTA 231
Score = 81.7 bits (172), Expect(7) = e-126
Identities = 30/45 (66%), Positives = 36/45 (80%)
Frame = -1 / +3
Query: 1164 KGSKSGASSRCAGCQGSGFKVQIRQLGPGMIQQMQHPCNECKGSG 1030
KGSKSG + RC GCQG+G K+ RQ+G GMIQQMQH C +CKG+G
Sbjct: 2400 KGSKSGTAGRCFGCQGTGMKITRRQIGLGMIQQMQHVCPDCKGTG 2534
Score = 79.0 bits (166), Expect(7) = e-126
Identities = 31/59 (52%), Positives = 41/59 (69%)
Frame = -1 / +2
Query: 555 LSPEQCKALEVVLPPKPVSQYTDMELDECEETMTYDVNIEEEMRRRQQHHQEAYDEDDD 379
LSP Q LE +LP K + E+D+CEET +DVNI EEM R++Q ++EAYD+DDD
Sbjct: 3302 LSPSQSLELEKILPQKTSKNLSQKEVDDCEETTLHDVNIAEEMSRKKQQYREAYDDDDD 3478
Score = 73.9 bits (155), Expect(7) = e-126
Identities = 29/37 (78%), Positives = 33/37 (89%)
Frame = -1 / +1
Query: 1269 EDVVHPLKVSLEDLYNGTSKKLSLSRSVLCSKCNGKG 1159
EDVVH +KVSLED+YNGT+KKLSLSR+ LCSKC G G
Sbjct: 1291 EDVVHSIKVSLEDVYNGTTKKLSLSRNALCSKCKGYG 1401
Score = 71.2 bits (149), Expect(7) = 2e-049
Identities = 34/45 (75%), Positives = 35/45 (77%)
Frame = +2 / -2
Query: 1472 LNFSGSPPLSGWFLMAALR*AFLRSSWDASLETPRIS*YRVLSLF 1606
L FSGSPPLSGWF +AA *AFL SS A ETPRIS*Y VLSLF
Sbjct: 215 LTFSGSPPLSGWFFIAAFL*AFLISSSLALFETPRIS*YLVLSLF 81
Score = 69.8 bits (146), Expect(7) = e-126
Identities = 27/31 (87%), Positives = 30/31 (96%)
Frame = -1 / +1
Query: 1479 KFKELAQAYEVLSDPEKREIYDQYGEDALKE 1387
+FKEL QAYEVLSDPEK+E+YDQYGEDALKE
Sbjct: 979 QFKELGQAYEVLSDPEKKELYDQYGEDALKE 1071
Score = 66.1 bits (138), Expect(5) = 4e-027
Identities = 27/42 (64%), Positives = 33/42 (78%)
Frame = -1 / +2
Query: 648 KAINDEGMPMYQRPFMKGKLYIHFSVEFPDSLSPEQCKALEV 523
KAINDEGMP + RPFMKG+LYI FSV+FPDS ++LE+
Sbjct: 3203 KAINDEGMPQHGRPFMKGRLYIKFSVDFPDSGFLSPSQSLEL 3328
Score = 60.2 bits (125), Expect(7) = e-126
Identities = 25/50 (50%), Positives = 30/50 (60%)
Frame = -1 / +2
Query: 1035 SGETISDKDRCPQCKGDXXXXXXXXXXXXXXKGMQNGQKITFPGEADEAV 886
+GE IS++DRCPQCKG+ KGMQ G KI F G+ADE V
Sbjct: 2609 AGEVISERDRCPQCKGNKITQEKKVLEVHVEKGMQQGHKIVFEGQADELV 2758
Score = 54.7 bits (113), Expect(7) = 2e-049
Identities = 25/50 (50%), Positives = 27/50 (54%)
Frame = +2 / -1
Query: 884 ITASSASPGNVIFCPFCIPXXXXXXXXXXXXXXLSPLHCGHLSLSLMVSP 1033
+T+SSA P N I P CIP L PLH GHLSLSLM SP
Sbjct: 2760 LTSSSACPSNTIL*PCCIPFSTCTSSTFFS*VILFPLH*GHLSLSLMTSP 2611
Score = 48.3 bits (99), Expect(5) = 1e-021
Identities = 21/37 (56%), Positives = 24/37 (64%)
Frame = +2 / -2
Query: 1160 PLPLHLEQRTLRDRESFFDVPLYKSSRETFRG*TTSS 1270
P PLH E + +SFF VPLY SS+ETF TTSS
Sbjct: 1400 PYPLHFEHSAFLESDSFFVVPLYTSSKETFIECTTSS 1290
Score = 46.4 bits (95), Expect(7) = 2e-049
Identities = 18/28 (64%), Positives = 20/28 (71%)
Frame = +2 / -3
Query: 659 GFTTSPGFDLISSCLLSK*VRTNWKPHR 742
G TSPGFDL +SCL S+ V NWKP R
Sbjct: 3109 GLMTSPGFDLTNSCLPSRCVTLNWKPQR 3026
Score = 41.8 bits (85), Expect(7) = 2e-049
Identities = 20/53 (37%), Positives = 24/53 (45%)
Frame = +2 / -3
Query: 1031 PEPLHSLQGXXXXXXXPGPNCRIWTLKPEPWQPAHLDEAPDFEPLPLHLEQRT 1189
P PL S Q P P C + P PW P HL P FEP ++ + RT
Sbjct: 2533 PVPLQSGQTCCIC*IMPSPICLLVIFIPVPWHPKHLPAVPLFEPFLINQKFRT 2375
Score = 41.4 bits (84), Expect(5) = 4e-027
Identities = 23/59 (38%), Positives = 32/59 (54%)
Frame = -3 / +2
Query: 853 *YYHWRYHLCSPAEGASQVQEKGR*PLLRAHTDLD*ISVWLPVCSDSLG*QAAADQIEP 677
*Y H R+ C ++G S+V E *P +L+* S+WLPV + +A QIEP
Sbjct: 2915 *YNHRRHSFCLASKGTSEVSEGA**PSH*TQFELN*CSLWLPV*CHTS*WKATIGQIEP 3091
Score = 41.4 bits (84), Expect(5) = 1e-021
Identities = 24/65 (36%), Positives = 33/65 (50%)
Frame = +1 / -2
Query: 661 LHNFTRVRFDQQLPVIQVSQNKLEATQRFSQGQCVLVEEVIAXXXXXXXXXXXENKDDIS 840
L +F VRFDQ LP I++ KLEAT+ S Q V +VI ++K+ +S
Sbjct: 3107 LDDFAGVRFDQ*LPSIKMCDIKLEATESIS*AQIVFNVKVITLPPKLRMSLYLQDKNYVS 2928
Query: 841 SGSIR 855
IR
Sbjct: 2927 CDCIR 2913
Score = 40.0 bits (81), Expect(7) = 2e-049
Identities = 19/31 (61%), Positives = 20/31 (64%)
Frame = +2 / -1
Query: 557 ESGNSTEK*MYSLPFMKGL*YMGMPSSLIAL 649
ESG ST MY PFM GL G+PSS IAL
Sbjct: 3294 ESGKSTLNLMYRRPFMNGLPCCGIPSSFIAL 3202
Score = 39.6 bits (80), Expect(5) = 1e-021
Identities = 18/51 (35%), Positives = 30/51 (58%)
Frame = +1 / -3
Query: 880 SHYCFISFARECDLLPVLHTFLNYHFKNFLLRHNFITFALWASILIADGLS 1032
S Y FIS + E +L+ +LH + H ++ L N ++ AL ASI + + L+
Sbjct: 2764 STYEFISLSFEYNLVTLLHPLFHMHLQHLLFLSNLVSLALRASISLTNDLA 2612
Score = 37.7 bits (76), Expect(7) = 2e-049
Identities = 17/36 (47%), Positives = 21/36 (58%)
Frame = +3 / -3
Query: 1206 ASLMCHCTNPPEKPLEDEPHPLPFAAFCPYCCHHLQ 1313
ASL C T+PP KPL + PH L + F HHL+
Sbjct: 1354 ASL*CRYTHPPRKPL*NAPHLLLASVFLRVKDHHLR 1247
Score = 35.9 bits (72), Expect(5) = 4e-027
Identities = 14/21 (66%), Positives = 16/21 (76%)
Frame = -2 / +2
Query: 1268 RMWFIL*RFLWRICTMAHQRS 1206
+MW IL*RF WR+C A QRS
Sbjct: 1292 KMWCIL*RFPWRMCITALQRS 1354
Score = 33.6 bits (67), Expect(5) = 1e-021
Identities = 14/37 (37%), Positives = 24/37 (64%)
Frame = +1 / -3
Query: 529 QGLALLRAQRVRELHRKVNVQLALHEGPLIHGHAFIV 639
Q LA + R+RE++ K++V +LHE P + H+ I+
Sbjct: 3322 QALAWGKKTRIREINTKLDV*ASLHERPTMLWHSLII 3212
Score = 33.6 bits (67), Expect(5) = 4e-027
Identities = 18/36 (50%), Positives = 21/36 (58%)
Frame = -3 / +3
Query: 1486 SREVQGASSGL*GPQ*S*KARDL*SIW*GCPQGGNG 1379
S VQG +* + S*K R +*SIW* CP NG
Sbjct: 972 SISVQGVGPSI*SVERS*KERTV*SIW*RCP*RRNG 1079
Score = 31.8 bits (63), Expect(7) = 2e-049
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = +1 / -1
Query: 1381 HSLLEGILTILIINLSLFRITE 1446
HS +GI TILII LFRI +
Sbjct: 1077 HSFFKGIFTILIIQFFLFRIAQ 1012
Score = 30.4 bits (60), Expect(5) = 4e-027
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = -2 / +1
Query: 1163 RVQSLELHRGVLVAKVLALRSKSGSWGLE*FSKCSILAMSAR 1038
+VQ +EL GVL A+ R G *F+KC+ ++A+
Sbjct: 2401 KVQKVELLEGVLDARAQV*RLPEGKLDWA*FNKCNTSVLTAK 2526
Score = 25.4 bits (49), Expect(5) = 1e-021
Identities = 10/24 (41%), Positives = 16/24 (66%)
Frame = +1 / -2
Query: 1036 TLALIARMLHLLNHSRPQLPDLDL 1107
+ A+ +LHLLNH++ LP +L
Sbjct: 2528 SFAVRTDVLHLLNHAQSNLPSGNL 2457
>gb|CO513103.1|CO513103 s13dSG89H0300028_122132 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 550
Score = 184 bits (396), Expect(2) = 1e-067
Identities = 75/81 (92%), Positives = 79/81 (97%)
Frame = -1 / +1
Query: 1629 QMFGRAPKKSDNTRYYEILGVSKDASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYE 1450
+MFGRAPKKSD+TRYYEILGVSK ASQDDLKKAY+KAAIKNHPDKGGDPEKFKELAQAYE
Sbjct: 79 KMFGRAPKKSDSTRYYEILGVSKTASQDDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYE 258
Query: 1449 VLSDPEKREIYDQYGEDALKE 1387
VLSDPEKR+IYD YGEDALKE
Sbjct: 259 VLSDPEKRDIYDTYGEDALKE 321
Score = 94.5 bits (200), Expect(2) = 7e-034
Identities = 47/80 (58%), Positives = 53/80 (66%)
Frame = +2 / -3
Query: 1388 SLRAXXXXXXXXXXXXGSLRTXXXXXXXLNFSGSPPLSGWFLMAALR*AFLRSSWDASLE 1567
SLRA GSL+T LNFSGSPPLSG FL+AA *AF +SS +A L+
Sbjct: 320 SLRASSPYVS*ISRFSGSLKTS*ACASSLNFSGSPPLSG*FLIAAFL*AFFKSS*EAVLD 141
Query: 1568 TPRIS*YRVLSLFFGARPNI 1627
TP+IS*Y VLSLFFGA PNI
Sbjct: 140 TPKIS*YLVLSLFFGALPNI 81
Score = 88.6 bits (187), Expect(2) = 1e-067
Identities = 37/40 (92%), Positives = 39/40 (97%)
Frame = -1 / +1
Query: 1269 EDVVHPLKVSLEDLYNGTSKKLSLSRSVLCSKCNGKGSKS 1150
EDVVHPLKVSLEDLY GTSKKLSLSR+VLCSKC+GKGSKS
Sbjct: 430 EDVVHPLKVSLEDLYLGTSKKLSLSRNVLCSKCSGKGSKS 549
Score = 65.2 bits (136), Expect(2) = 7e-034
Identities = 27/40 (67%), Positives = 31/40 (77%)
Frame = +2 / -3
Query: 1151 DFEPLPLHLEQRTLRDRESFFDVPLYKSSRETFRG*TTSS 1270
D EPLPLH E +T +RESF DVP +SS+ET RG*TTSS
Sbjct: 548 DLEPLPLHFEHKTFLERESFLDVPR*RSSKETLRG*TTSS 429
>emb|Z71997.1|MSDNAJLP M.sativa mRNA for DnaJ-like protein
Length = 513
Score = 182 bits (393), Expect(2) = 1e-053
Identities = 69/95 (72%), Positives = 82/95 (86%)
Frame = -1 / +3
Query: 1269 EDVVHPLKVSLEDLYNGTSKKLSLSRSVLCSKCNGKGSKSGASSRCAGCQGSGFKVQIRQ 1090
EDVVH +KVSLED+YNGT+KKLSLSR+ LCSKC GKGSKSG + RC GCQG+G K+ RQ
Sbjct: 177 EDVVHSIKVSLEDVYNGTTKKLSLSRNALCSKCKGKGSKSGTAGRCFGCQGTGMKITRRQ 356
Query: 1089 LGPGMIQQMQHPCNECKGSGETISDKDRCPQCKGD 985
+G GMIQQMQH C +CKG+GE IS++DRCPQCKG+
Sbjct: 357 IGLGMIQQMQHVCPDCKGTGEVISERDRCPQCKGN 461
Score = 109 bits (232), Expect(2) = 5e-028
Identities = 51/96 (53%), Positives = 55/96 (57%)
Frame = +2 / -3
Query: 983 LSPLHCGHLSLSLMVSPEPLHSLQGXXXXXXXPGPNCRIWTLKPEPWQPAHLDEAPDFEP 1162
L PLH GHLSLSLM SP PL S Q P P C + P PW P HL P FEP
Sbjct: 463 LFPLH*GHLSLSLMTSPVPLQSGQTCCIC*IMPSPICLLVIFIPVPWHPKHLPAVPLFEP 284
Query: 1163 LPLHLEQRTLRDRESFFDVPLYKSSRETFRG*TTSS 1270
PLH E + +SFF VPLY SS+ETF TTSS
Sbjct: 283 FPLHFEHSAFLESDSFFVVPLYTSSKETFIECTTSS 176
Score = 43.2 bits (88), Expect(2) = 1e-053
Identities = 16/18 (88%), Positives = 17/18 (94%)
Frame = -1 / +3
Query: 1440 DPEKREIYDQYGEDALKE 1387
DPEK+E YDQYGEDALKE
Sbjct: 6 DPEKKESYDQYGEDALKE 59
Score = 30.9 bits (61), Expect(2) = 5e-028
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = +1 / -2
Query: 1381 HSLLEGILTILIINLSLFRI 1440
HS +GI TILII LFRI
Sbjct: 65 HSFFKGIFTILIIRFFLFRI 6
>gb|AW698418.1|AW698418 G266 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 273
Score = 138 bits (296), Expect = 2e-033
Identities = 56/85 (65%), Positives = 67/85 (78%)
Frame = -1 / -2
Query: 618 YQRPFMKGKLYIHFSVEFPDSLSPEQCKALEVVLPPKPVSQYTDMELDECEETMTYDVNI 439
YQRPFMKGKLYIHF+VEFPD+LS +Q K L+ VLP KP SQ TDME+DECEET +DVN+
Sbjct: 266 YQRPFMKGKLYIHFTVEFPDTLSLDQVKGLQAVLPAKPSSQLTDMEIDECEETTLHDVNM 87
Query: 438 EEEMRRRQQHHQEAYDEDDDVPGGG 364
EEE RR+QQ Q+ ++DD GG
Sbjct: 86 EEENRRKQQQQQQEAYDEDDDMPGG 12
>gb|AW698402.1|AW698402 G249 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 316
Score = 127 bits (272), Expect = 4e-030
Identities = 53/86 (61%), Positives = 66/86 (76%)
Frame = -1 / -1
Query: 621 MYQRPFMKGKLYIHFSVEFPDSLSPEQCKALEVVLPPKPVSQYTDMELDECEETMTYDVN 442
+YQRPFMKGKLYIHF+VEF D+LS +Q K L+ V+P KP SQ TDME+DECEET +DVN
Sbjct: 289 VYQRPFMKGKLYIHFTVEFTDTLSLDQVKGLQDVIPGKPSSQLTDMEIDECEETTLHDVN 110
Query: 441 IEEEMRRRQQHHQEAYDEDDDVPGGG 364
+EEE R +QQ Q+ ++DD GG
Sbjct: 109 MEEENRSKQQQQQQ*AYDEDDDMLGG 32
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,509,983
Number of Sequences: 7669
Number of extensions: 161055
Number of successful extensions: 987
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 453743736
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)