TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161295.2.1
(1475 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516282.1|CO516282 s13dSG69E1000081_445892 Glandular tr... 26 0.12
gb|CO517165.1|CO517165 s13dSG33D0400042_474414 Glandular tr... 26 0.65
gb|CO511838.1|CO511838 s13dSG02E1200099_103576 Glandular tr... 29 2.1
gb|CO512768.1|CO512768 s13dSG88D0900074_121462 Glandular tr... 28 2.8
gb|CO515666.1|CO515666 s13dSG60D0300030_419481 Glandular tr... 28 2.8
gb|CO516690.1|CO516690 s13dSG74B1200101_446708 Glandular tr... 28 2.8
gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein Ms... 28 2.8
emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like pr... 28 2.8
emb|X52398.1|MSCAL1 Alfalfa cal1 mRNA for calmodulin 28 2.8
gb|CO517195.1|CO517195 s13dSG33G0900072_474474 Glandular tr... 25 3.7
gb|CO514365.1|CO514365 s13dSG84F0800063_157392 Glandular tr... 28 3.9
gb|CO515511.1|CO515511 s13dSG52E0600051_418081 Glandular tr... 28 3.9
gb|AF079404.1|AF079404 Medicago sativa subsp. X varia cell ... 28 3.9
emb|AX008931.1| Sequence 1 from Patent WO9964451 28 3.9
dbj|BD210105.1| Plant protein having repeated WD40 motif, n... 28 3.9
emb|AJ251210.1|MSA251210 Medicago sativa mRNA for rac G-Pro... 28 3.9
emb|AJ619853.1| Medicago sativa mRNA for small GTPase Rac4 ... 28 3.9
emb|AJ966571.1| Medicago sativa subsp. x varia mRNA for put... 28 3.9
emb|AJ966572.1| Medicago sativa subsp. x varia mRNA for put... 28 3.9
emb|AJ966573.1| Medicago sativa subsp. x varia mRNA for put... 28 3.9
gb|CO514757.1|CO514757 s13dSG55G1000084_328174 Glandular tr... 27 5.3
gb|CO516209.1|CO516209 s13dSG67G0300020_445746 Glandular tr... 27 5.3
gb|CO516540.1|CO516540 s13dSG57C0800056_446408 Glandular tr... 27 5.3
gb|CO513296.1|CO513296 s13dSG37D0200014_129756 Glandular tr... 27 7.3
gb|CO514636.1|CO514636 s13dSG46C0200018_327932 Glandular tr... 27 7.3
gb|CO516849.1|CO516849 s13dSG98E0400024_447026 Glandular tr... 27 7.3
>gb|CO516282.1|CO516282 s13dSG69E1000081_445892 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 544
Score = 25.8 bits (50), Expect(2) = 0.12
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = +3 / -2
Query: 822 NPWSTFQLYACPHRNS 869
NPWS +CPHR S
Sbjct: 225 NPWSYALPQSCPHRPS 178
Score = 24.0 bits (46), Expect(2) = 0.12
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +3 / -2
Query: 660 NSWFAFHHHKPSRAPSSVPEQQSY*QHGHRQC 755
NSW H + P P + + + HR+C
Sbjct: 339 NSWSTQHANFPQACPETQTYSDNQRETQHRRC 244
>gb|CO517165.1|CO517165 s13dSG33D0400042_474414 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 601
Score = 25.8 bits (50), Expect(2) = 0.65
Identities = 8/18 (44%), Positives = 12/18 (66%)
Frame = +2 / +1
Query: 2 FFFFFLQNDRIYTIYMML 55
FFFFF ++IY Y ++
Sbjct: 76 FFFFFFYTNKIYNSYSII 129
Score = 24.4 bits (47), Expect(2) = 0.65
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = +2 / +2
Query: 941 TSLVLVRRNLALILFANPSMFRVPTTLVLCY 1033
+SL ++ RNL LF N + + T CY
Sbjct: 401 SSLRVITRNLLFDLFQNTFLLSLIMTSCCCY 493
>gb|CO511838.1|CO511838 s13dSG02E1200099_103576 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 559
Score = 28.6 bits (56), Expect = 2.1
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +3 / -3
Query: 669 FAFHHHKPSRAPSSVPEQQSY*QHGHRQCTCE 764
FA HHH+ ++ S P Q Q+ H++ CE
Sbjct: 551 FAGHHHQRVQSQSFQPASQLDFQY*HQRILCE 456
>gb|CO512768.1|CO512768 s13dSG88D0900074_121462 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 590
Score = 28.1 bits (55), Expect = 2.8
Identities = 8/15 (53%), Positives = 9/15 (60%)
Frame = +3 / +2
Query: 912 PAGAGEDHHRPHWYW 956
P G GED H W+W
Sbjct: 443 PGGEGEDEHDVVWFW 487
>gb|CO515666.1|CO515666 s13dSG60D0300030_419481 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 513
Score = 28.1 bits (55), Expect = 2.8
Identities = 7/12 (58%), Positives = 9/12 (75%)
Frame = +3 / -2
Query: 657 GNSWFAFHHHKP 692
GN W+ FH H+P
Sbjct: 284 GNQWYRFHQHQP 249
>gb|CO516690.1|CO516690 s13dSG74B1200101_446708 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 567
Score = 28.1 bits (55), Expect = 2.8
Identities = 7/12 (58%), Positives = 9/12 (75%)
Frame = +3 / -2
Query: 657 GNSWFAFHHHKP 692
GN W+ FH H+P
Sbjct: 284 GNQWYRFHQHQP 249
>gb|AF069507.1|AF069507 Medicago sativa DnaJ-like protein MsJ1 gene, complete cds
Length = 4449
Score = 28.1 bits (55), Expect = 2.8
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = -2 / -1
Query: 553 CQGCPGHHRPQRTDRVP 503
C C GHH P T VP
Sbjct: 3006 CVQCEGHHAPSETSDVP 2956
>emb|AJ000995.1|MSDNAJ Medicago sativa mRNA for DnaJ-like protein
Length = 1545
Score = 28.1 bits (55), Expect = 2.8
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = -2 / -3
Query: 553 CQGCPGHHRPQRTDRVP 503
C C GHH P T VP
Sbjct: 892 CVQCEGHHAPSETSDVP 842
>emb|X52398.1|MSCAL1 Alfalfa cal1 mRNA for calmodulin
Length = 807
Score = 28.1 bits (55), Expect = 2.8
Identities = 7/12 (58%), Positives = 9/12 (75%)
Frame = +3 / -1
Query: 657 GNSWFAFHHHKP 692
GN W+ FH H+P
Sbjct: 246 GNQWYRFHQHQP 211
>gb|CO517195.1|CO517195 s13dSG33G0900072_474474 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 123
Score = 24.9 bits (48), Expect(2) = 3.7
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +3 / +3
Query: 3 FFFFFCKMTGFI 38
FFFFFC+ FI
Sbjct: 42 FFFFFCQTENFI 77
Score = 18.9 bits (35), Expect(2) = 3.7
Identities = 7/14 (50%), Positives = 9/14 (64%)
Frame = +1 / +1
Query: 397 EGKALTQGDLRFPP 438
+GKAL +FPP
Sbjct: 79 KGKALINTQDKFPP 120
>gb|CO514365.1|CO514365 s13dSG84F0800063_157392 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 461
Score = 27.6 bits (54), Expect = 3.9
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3 / +3
Query: 198 YSSNTFPTNYVAHSF 242
Y+SNTFPT+YV F
Sbjct: 273 YTSNTFPTDYVPTVF 317
>gb|CO515511.1|CO515511 s13dSG52E0600051_418081 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 211
Score = 27.6 bits (54), Expect = 3.9
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = -3 / -3
Query: 1254 STTQKLTSKWPLPFPLSKQL 1195
S TQ KWP+P+ LS+++
Sbjct: 80 SNTQPSARKWPVPYQLSQRV 21
>gb|AF079404.1|AF079404 Medicago sativa subsp. X varia cell cycle switch protein (ccs52)
mRNA, complete cds
Length = 1988
Score = 27.6 bits (54), Expect = 3.9
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +1 / -1
Query: 280 GPPKISMSCALLHP 321
GPP I SC LLHP
Sbjct: 929 GPPLILSSCILLHP 888
>emb|AX008931.1| Sequence 1 from Patent WO9964451
Length = 2006
Score = 27.6 bits (54), Expect = 3.9
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +1 / -1
Query: 280 GPPKISMSCALLHP 321
GPP I SC LLHP
Sbjct: 929 GPPLILSSCILLHP 888
>dbj|BD210105.1| Plant protein having repeated WD40 motif, nucleic acid encoding the
protein and utilization of the same
Length = 2006
Score = 27.6 bits (54), Expect = 3.9
Identities = 10/14 (71%), Positives = 10/14 (71%)
Frame = +1 / -1
Query: 280 GPPKISMSCALLHP 321
GPP I SC LLHP
Sbjct: 929 GPPLILSSCILLHP 888
>emb|AJ251210.1|MSA251210 Medicago sativa mRNA for rac G-Protein (rac1 gene)
Length = 917
Score = 27.6 bits (54), Expect = 3.9
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3 / +3
Query: 198 YSSNTFPTNYVAHSF 242
Y+SNTFPT+YV F
Sbjct: 105 YTSNTFPTDYVPTVF 149
>emb|AJ619853.1| Medicago sativa mRNA for small GTPase Rac4 (rac4 gene)
Length = 594
Score = 27.6 bits (54), Expect = 3.9
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3 / +1
Query: 198 YSSNTFPTNYVAHSF 242
Y+SNTFPT+YV F
Sbjct: 76 YTSNTFPTDYVPTVF 120
>emb|AJ966571.1| Medicago sativa subsp. x varia mRNA for putative Rho GTPase (rop5
gene)
Length = 1104
Score = 27.6 bits (54), Expect = 3.9
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3 / +3
Query: 198 YSSNTFPTNYVAHSF 242
Y+SNTFPT+YV F
Sbjct: 252 YTSNTFPTDYVPTVF 296
>emb|AJ966572.1| Medicago sativa subsp. x varia mRNA for putative Rho GTPase (rop3
gene)
Length = 1106
Score = 27.6 bits (54), Expect = 3.9
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3 / +2
Query: 198 YSSNTFPTNYVAHSF 242
Y+SNTFPT+YV F
Sbjct: 269 YTSNTFPTDYVPTVF 313
>emb|AJ966573.1| Medicago sativa subsp. x varia mRNA for putative Rho GTPase (rop6
gene)
Length = 1266
Score = 27.6 bits (54), Expect = 3.9
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +3 / +1
Query: 198 YSSNTFPTNYVAHSF 242
Y+SNTFPT+YV F
Sbjct: 193 YTSNTFPTDYVPTVF 237
>gb|CO514757.1|CO514757 s13dSG55G1000084_328174 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 505
Score = 27.2 bits (53), Expect = 5.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +1 / +3
Query: 694 QGLLPQCLSNKVTDNTAIVNAHARAICIED 783
Q LL QCLSNK+ + A C+ED
Sbjct: 375 QRLLLQCLSNKLPKCDICQDKPAFIFCVED 464
>gb|CO516209.1|CO516209 s13dSG67G0300020_445746 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 459
Score = 27.2 bits (53), Expect = 5.3
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2 / -1
Query: 533 VSWTTLASSSMVNSPGLPRLNGPTCSPSISFIRP 634
V WT+ + + ++ LP NGP CS + I P
Sbjct: 102 VQWTSESHYNAMSHFDLPIFNGPFCSSTAITIIP 1
>gb|CO516540.1|CO516540 s13dSG57C0800056_446408 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 520
Score = 27.2 bits (53), Expect = 5.3
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +1 / +1
Query: 694 QGLLPQCLSNKVTDNTAIVNAHARAICIED 783
Q LL QCLSNK+ + A C+ED
Sbjct: 55 QRLLLQCLSNKLPKCDICQDKPAFIFCVED 144
>gb|CO513296.1|CO513296 s13dSG37D0200014_129756 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 584
Score = 26.7 bits (52), Expect = 7.3
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = +2 / +1
Query: 947 LVLVRRNLALILFANPSMFRVPTTL 1021
LVL RRN + L ++P + +PT L
Sbjct: 16 LVLTRRNTSQSLISSPMLAALPTAL 90
>gb|CO514636.1|CO514636 s13dSG46C0200018_327932 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 550
Score = 26.7 bits (52), Expect = 7.3
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = +2 / +2
Query: 359 WSPKMRLRKSETMRGRPSRRGIFGSHPRNS 448
W + + +K+ RGR G FGSH S
Sbjct: 14 WYLRKKKKKNGGDRGRKRSEGAFGSHKAQS 103
>gb|CO516849.1|CO516849 s13dSG98E0400024_447026 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 445
Score = 26.7 bits (52), Expect = 7.3
Identities = 12/22 (54%), Positives = 13/22 (59%)
Frame = +2 / +2
Query: 338 GVVAVASWSPKMRLRKSETMRG 403
G V SW P + LRK ET RG
Sbjct: 182 GTVWPESWMP*LTLRK*ETSRG 247
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,609,193
Number of Sequences: 7669
Number of extensions: 199564
Number of successful extensions: 1438
Number of sequences better than 10.0: 28
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 407276004
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)