TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161238.2.2
(1186 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA... 110 7e-067
gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA... 110 7e-067
gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, comple... 110 1e-059
gb|CO513464.1|CO513464 s13dSG10E0500035_130092 Glandular tr... 101 2e-042
gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular tr... 83 3e-021
>gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA, complete cds
Length = 1267
Score = 110 bits (234), Expect(4) = 7e-067
Identities = 39/58 (67%), Positives = 48/58 (82%)
Frame = +1 / +2
Query: 559 QWPCAAGQKYYGRGPLQISWNYNYGPAGRAIGFDGLGDPGRVARDAVVAFKAALWFWM 732
++PCA+G++YYGRGP+QISWNYNYG GRAIG D L +P VA D V++FK ALWFWM
Sbjct: 545 EFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWM 718
Score = 75.3 bits (158), Expect(4) = 7e-067
Identities = 30/58 (51%), Positives = 38/58 (65%)
Frame = +1 / +2
Query: 322 FFNGIKNQAGSGCEGKNFYTRSAFLSAVKAYPGFAHGGSQVQGKREIAAFFAHATHET 495
F N +K++ SGC+GK YT AF+SA KA+P FA+ G KREIAAF +HET
Sbjct: 275 FNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAAFLGQTSHET 448
Score = 61.6 bits (128), Expect(4) = 5e-026
Identities = 32/57 (56%), Positives = 34/57 (59%)
Frame = -3 / -2
Query: 731 IQNQSAALNATTASRATLPGSPSPSKPMALPAGP*L*FHEICSGPRP*YFCPAAHGH 561
IQN A L T S AT G S S P+ALP P*L*F EI GP P*Y P AHG+
Sbjct: 717 IQNHRAVLKDITGSVATRSGLLSKSTPIALPHCP*L*FQEIWMGPLP*YCFPEAHGN 547
Score = 61.1 bits (127), Expect(4) = 7e-067
Identities = 22/47 (46%), Positives = 29/47 (61%)
Frame = +1 / +2
Query: 760 GFGATTRAINGALECGGNNPAQMNARVGYYRQYCRQLGVDPGPNLTC 900
G+G T ING LECG ++ R+G+Y++YC LGV G NL C
Sbjct: 809 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDC 949
Score = 60.2 bits (125), Expect(4) = 7e-067
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = +3 / +2
Query: 168 CQPNVCCSKFGYCGTTDEYCGDGCQS 245
C +CCSKFG+CG+T EYCGDGCQS
Sbjct: 137 CPGGLCCSKFGWCGSTGEYCGDGCQS 214
Score = 40.9 bits (83), Expect(4) = 5e-026
Identities = 21/47 (44%), Positives = 23/47 (48%)
Frame = -3 / -2
Query: 899 QVRLGPGSTPSWRQYCL**PTRAFIWAGLFPPHSRAPLMALVVAPNP 759
Q RL P TPS QY L* P R+ PHS P + LV P P
Sbjct: 948 QSRLAPYPTPSMSQYLL*NPIRSCTLPSCPLPHSSPPFIMLVTVPYP 808
Score = 33.1 bits (66), Expect(4) = 5e-026
Identities = 25/58 (43%), Positives = 26/58 (44%)
Frame = -3 / -2
Query: 494 VSCVACAKKAAISRLPCTCDPPWAKPGYALTALRNALRV*KFLPSHPLPAWFLMPLKK 321
VSC KKAAIS P AK G AL A A V P HPL + L L K
Sbjct: 447 VSCDV*PKKAAISLFLVAVSPLLAKLGNALAAEMKAS*VYNPFP*HPLSSRCLSILLK 274
Score = 31.8 bits (63), Expect(4) = 5e-026
Identities = 13/17 (76%), Positives = 13/17 (76%)
Frame = -1 / -2
Query: 235 PSPQYSSVVPQ*PNLLQ 185
PSPQYS V P PNLLQ
Sbjct: 204 PSPQYSPVDPHQPNLLQ 154
>gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA, complete cds
Length = 1259
Score = 110 bits (234), Expect(4) = 7e-067
Identities = 39/58 (67%), Positives = 48/58 (82%)
Frame = +1 / +1
Query: 559 QWPCAAGQKYYGRGPLQISWNYNYGPAGRAIGFDGLGDPGRVARDAVVAFKAALWFWM 732
++PCA+G++YYGRGP+QISWNYNYG GRAIG D L +P VA D V++FK ALWFWM
Sbjct: 544 EFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWM 717
Score = 75.3 bits (158), Expect(4) = 7e-067
Identities = 30/58 (51%), Positives = 38/58 (65%)
Frame = +1 / +1
Query: 322 FFNGIKNQAGSGCEGKNFYTRSAFLSAVKAYPGFAHGGSQVQGKREIAAFFAHATHET 495
F N +K++ SGC+GK YT AF+SA KA+P FA+ G KREIAAF +HET
Sbjct: 274 FNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAAFLGQTSHET 447
Score = 61.6 bits (128), Expect(4) = 5e-026
Identities = 32/57 (56%), Positives = 34/57 (59%)
Frame = -3 / -1
Query: 731 IQNQSAALNATTASRATLPGSPSPSKPMALPAGP*L*FHEICSGPRP*YFCPAAHGH 561
IQN A L T S AT G S S P+ALP P*L*F EI GP P*Y P AHG+
Sbjct: 716 IQNHRAVLKDITGSVATRSGLLSKSTPIALPHCP*L*FQEIWMGPLP*YCFPEAHGN 546
Score = 61.1 bits (127), Expect(4) = 7e-067
Identities = 22/47 (46%), Positives = 29/47 (61%)
Frame = +1 / +1
Query: 760 GFGATTRAINGALECGGNNPAQMNARVGYYRQYCRQLGVDPGPNLTC 900
G+G T ING LECG ++ R+G+Y++YC LGV G NL C
Sbjct: 808 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDC 948
Score = 60.2 bits (125), Expect(4) = 7e-067
Identities = 19/26 (73%), Positives = 22/26 (84%)
Frame = +3 / +1
Query: 168 CQPNVCCSKFGYCGTTDEYCGDGCQS 245
C +CCSKFG+CG+T EYCGDGCQS
Sbjct: 136 CPGGLCCSKFGWCGSTGEYCGDGCQS 213
Score = 40.9 bits (83), Expect(4) = 5e-026
Identities = 21/47 (44%), Positives = 23/47 (48%)
Frame = -3 / -1
Query: 899 QVRLGPGSTPSWRQYCL**PTRAFIWAGLFPPHSRAPLMALVVAPNP 759
Q RL P TPS QY L* P R+ PHS P + LV P P
Sbjct: 947 QSRLAPYPTPSMSQYLL*NPIRSCTLPSCPLPHSSPPFIMLVTVPYP 807
Score = 33.1 bits (66), Expect(4) = 5e-026
Identities = 25/58 (43%), Positives = 26/58 (44%)
Frame = -3 / -1
Query: 494 VSCVACAKKAAISRLPCTCDPPWAKPGYALTALRNALRV*KFLPSHPLPAWFLMPLKK 321
VSC KKAAIS P AK G AL A A V P HPL + L L K
Sbjct: 446 VSCDV*PKKAAISLFLVAVSPLLAKLGNALAAEMKAS*VYNPFP*HPLSSRCLSILLK 273
Score = 31.8 bits (63), Expect(4) = 5e-026
Identities = 13/17 (76%), Positives = 13/17 (76%)
Frame = -1 / -1
Query: 235 PSPQYSSVVPQ*PNLLQ 185
PSPQYS V P PNLLQ
Sbjct: 203 PSPQYSPVDPHQPNLLQ 153
>gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, complete cds
Length = 987
Score = 110 bits (234), Expect(4) = 1e-059
Identities = 39/58 (67%), Positives = 48/58 (82%)
Frame = +1 / +1
Query: 559 QWPCAAGQKYYGRGPLQISWNYNYGPAGRAIGFDGLGDPGRVARDAVVAFKAALWFWM 732
++PCA+G++YYGRGP+QISWNYNYG GRAIG D L +P VA D V++FK ALWFWM
Sbjct: 514 EFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWM 687
Score = 61.1 bits (127), Expect(4) = 1e-059
Identities = 22/47 (46%), Positives = 29/47 (61%)
Frame = +1 / +1
Query: 760 GFGATTRAINGALECGGNNPAQMNARVGYYRQYCRQLGVDPGPNLTC 900
G+G T ING LECG ++ R+G+Y++YC LGV G NL C
Sbjct: 778 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDC 918
Score = 59.7 bits (124), Expect(4) = 1e-059
Identities = 24/50 (48%), Positives = 31/50 (62%)
Frame = +1 / +1
Query: 322 FFNGIKNQAGSGCEGKNFYTRSAFLSAVKAYPGFAHGGSQVQGKREIAAF 471
F N +K++ SGC+GK YT AF+SA KA+P FA+ G KRE F
Sbjct: 241 FNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREDCCF 390
Score = 58.3 bits (121), Expect(4) = 1e-059
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = +3 / +1
Query: 168 CQPNVCCSKFGYCGTTDEYCGDGCQ 242
C +CCSKFG+CG+T EYCGDGCQ
Sbjct: 103 CPGGLCCSKFGWCGSTGEYCGDGCQ 177
>gb|CO513464.1|CO513464 s13dSG10E0500035_130092 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 587
Score = 101 bits (215), Expect(3) = 2e-042
Identities = 42/68 (61%), Positives = 48/68 (70%)
Frame = +1 / +2
Query: 322 FFNGIKNQAGSGCEGKNFYTRSAFLSAVKAYPGFAHGGSQVQGKREIAAFFAHATHETGH 501
FFN I +QA S C GKNFY+R+AFL A+ +Y F GS KREIAA FAH THET H
Sbjct: 314 FFNSIIDQADSSCAGKNFYSRAAFLDALNSYNQFGRSGSVDDSKREIAAAFAHFTHETLH 493
Query: 502 FCYISEIN 525
FCYI EI+
Sbjct: 494 FCYIEEID 517
Score = 73.9 bits (155), Expect(3) = 2e-042
Identities = 24/43 (55%), Positives = 31/43 (72%)
Frame = +3 / +2
Query: 123 LLCAVAGPAAAQNCGCQPNVCCSKFGYCGTTDEYCGDGCQSGP 251
++ V+ A+AQNCGC+ +CCS+ GYCG T YCG GCQ GP
Sbjct: 107 IMIMVSKNASAQNCGCEEGLCCSEHGYCGNTAAYCGTGCQQGP 235
Score = 30.9 bits (61), Expect(3) = 2e-042
Identities = 10/21 (47%), Positives = 13/21 (61%)
Frame = +1 / +2
Query: 529 SNAYCDPSKRQWPCAAGQKYY 591
S YCD S ++PCA + YY
Sbjct: 524 SKDYCDESNTKYPCAPNKGYY 586
>gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 510
Score = 82.6 bits (174), Expect(2) = 3e-021
Identities = 32/76 (42%), Positives = 44/76 (57%)
Frame = +1 / +1
Query: 544 DPSKRQWPCAAGQKYYGRGPLQISWNYNYGPAGRAIGFDGLGDPGRVARDAVVAFKAALW 723
D K +PC G +YYGRG + I WNYNYG AG A+ + L P + ++A +AF+AA+W
Sbjct: 28 DYYKLTYPCTPGAEYYGRGAIPIYWNYNYGAAGEALKVNLLDHPEYIEQNATLAFQAAIW 207
Query: 724 FWMNSVHGVVPQGFGA 771
WM + P A
Sbjct: 208 KWMTPIKKSQPSAHDA 255
Score = 34.1 bits (68), Expect(2) = 3e-021
Identities = 16/39 (41%), Positives = 19/39 (48%)
Frame = +1 / +1
Query: 760 GFGATTRAINGALECGGNNPAQMNARVGYYRQYCRQLGV 876
GFGAT + G CG + MN V +Y Y LGV
Sbjct: 310 GFGATMNILYGEGVCGQGDVDSMNTIVSHYLYYLDLLGV 426
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,596,353
Number of Sequences: 7669
Number of extensions: 78456
Number of successful extensions: 810
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 322499151
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)