TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2161238.2.1
(1225 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA... 107 6e-063
gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA... 107 6e-063
gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, comple... 107 1e-055
gb|CO513464.1|CO513464 s13dSG10E0500035_130092 Glandular tr... 113 3e-046
>gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA, complete cds
Length = 1267
Score = 107 bits (229), Expect(4) = 6e-063
Identities = 39/58 (67%), Positives = 49/58 (84%)
Frame = -3 / +2
Query: 653 QWPCAAGQKYYGRGPLQISWNYNYGPAGRDIGFNGLADPNRVAQDAVIAFKTALWFWM 480
++PCA+G++YYGRGP+QISWNYNYG GR IG + L +P+ VA D VI+FKTALWFWM
Sbjct: 545 EFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWM 718
Score = 73.0 bits (153), Expect(4) = 6e-063
Identities = 29/60 (48%), Positives = 37/60 (61%)
Frame = -3 / +2
Query: 896 DAFFNGIKNQAGSGCEGKNFYTRSAFLSAVNKYPGFAHGGTEVEGKREIAAFFAHVTHET 717
D F N +K++ SGC+GK YT AF+SA +P FA+ G KREIAAF +HET
Sbjct: 269 DTFNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAAFLGQTSHET 448
Score = 58.3 bits (121), Expect(4) = 6e-063
Identities = 18/26 (69%), Positives = 21/26 (80%)
Frame = -3 / +2
Query: 1055 CQPNFCCSKFGYCGTTDDYCGDGCQS 978
C CCSKFG+CG+T +YCGDGCQS
Sbjct: 137 CPGGLCCSKFGWCGSTGEYCGDGCQS 214
Score = 54.2 bits (112), Expect(4) = 6e-063
Identities = 20/47 (42%), Positives = 26/47 (55%)
Frame = -3 / +2
Query: 452 GFGATIRAINGALECNGNNPAQMNARVGYYKQYCQQLRVDPGPNLTC 312
G+G ING LEC ++ R+G+YK+YC L V G NL C
Sbjct: 809 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDC 949
>gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA, complete cds
Length = 1259
Score = 107 bits (229), Expect(4) = 6e-063
Identities = 39/58 (67%), Positives = 49/58 (84%)
Frame = -3 / +1
Query: 653 QWPCAAGQKYYGRGPLQISWNYNYGPAGRDIGFNGLADPNRVAQDAVIAFKTALWFWM 480
++PCA+G++YYGRGP+QISWNYNYG GR IG + L +P+ VA D VI+FKTALWFWM
Sbjct: 544 EFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWM 717
Score = 73.0 bits (153), Expect(4) = 6e-063
Identities = 29/60 (48%), Positives = 37/60 (61%)
Frame = -3 / +1
Query: 896 DAFFNGIKNQAGSGCEGKNFYTRSAFLSAVNKYPGFAHGGTEVEGKREIAAFFAHVTHET 717
D F N +K++ SGC+GK YT AF+SA +P FA+ G KREIAAF +HET
Sbjct: 268 DTFNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREIAAFLGQTSHET 447
Score = 58.3 bits (121), Expect(4) = 6e-063
Identities = 18/26 (69%), Positives = 21/26 (80%)
Frame = -3 / +1
Query: 1055 CQPNFCCSKFGYCGTTDDYCGDGCQS 978
C CCSKFG+CG+T +YCGDGCQS
Sbjct: 136 CPGGLCCSKFGWCGSTGEYCGDGCQS 213
Score = 54.2 bits (112), Expect(4) = 6e-063
Identities = 20/47 (42%), Positives = 26/47 (55%)
Frame = -3 / +1
Query: 452 GFGATIRAINGALECNGNNPAQMNARVGYYKQYCQQLRVDPGPNLTC 312
G+G ING LEC ++ R+G+YK+YC L V G NL C
Sbjct: 808 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDC 948
>gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, complete cds
Length = 987
Score = 107 bits (229), Expect(4) = 1e-055
Identities = 39/58 (67%), Positives = 49/58 (84%)
Frame = -3 / +1
Query: 653 QWPCAAGQKYYGRGPLQISWNYNYGPAGRDIGFNGLADPNRVAQDAVIAFKTALWFWM 480
++PCA+G++YYGRGP+QISWNYNYG GR IG + L +P+ VA D VI+FKTALWFWM
Sbjct: 514 EFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWM 687
Score = 57.4 bits (119), Expect(4) = 1e-055
Identities = 23/52 (44%), Positives = 30/52 (57%)
Frame = -3 / +1
Query: 896 DAFFNGIKNQAGSGCEGKNFYTRSAFLSAVNKYPGFAHGGTEVEGKREIAAF 741
D F N +K++ SGC+GK YT AF+SA +P FA+ G KRE F
Sbjct: 235 DTFNNMLKHRDDSGCQGKGLYTYDAFISAAKAFPNFANNGDTATKKREDCCF 390
Score = 56.5 bits (117), Expect(4) = 1e-055
Identities = 17/25 (68%), Positives = 20/25 (80%)
Frame = -3 / +1
Query: 1055 CQPNFCCSKFGYCGTTDDYCGDGCQ 981
C CCSKFG+CG+T +YCGDGCQ
Sbjct: 103 CPGGLCCSKFGWCGSTGEYCGDGCQ 177
Score = 54.2 bits (112), Expect(4) = 1e-055
Identities = 20/47 (42%), Positives = 26/47 (55%)
Frame = -3 / +1
Query: 452 GFGATIRAINGALECNGNNPAQMNARVGYYKQYCQQLRVDPGPNLTC 312
G+G ING LEC ++ R+G+YK+YC L V G NL C
Sbjct: 778 GYGTVTNIINGGLECGRGQDGRVQDRIGFYKRYCDILGVGYGANLDC 918
>gb|CO513464.1|CO513464 s13dSG10E0500035_130092 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 587
Score = 113 bits (242), Expect(3) = 3e-046
Identities = 46/77 (59%), Positives = 56/77 (72%)
Frame = -3 / +2
Query: 917 NVANVVSDAFFNGIKNQAGSGCEGKNFYTRSAFLSAVNKYPGFAHGGTEVEGKREIAAFF 738
NVA++V+ FFN I +QA S C GKNFY+R+AFL A+N Y F G+ + KREIAA F
Sbjct: 287 NVADIVTQEFFNSIIDQADSSCAGKNFYSRAAFLDALNSYNQFGRSGSVDDSKREIAAAF 466
Query: 737 AHVTHETGHFCYISEIN 687
AH THET HFCYI EI+
Sbjct: 467 AHFTHETLHFCYIEEID 517
Score = 71.6 bits (150), Expect(3) = 3e-046
Identities = 22/33 (66%), Positives = 24/33 (72%)
Frame = -3 / +2
Query: 1067 QNCGCQPNFCCSKFGYCGTTDDYCGDGCQSGPC 969
QNCGC+ CCS+ GYCG T YCG GCQ GPC
Sbjct: 140 QNCGCEEGLCCSEHGYCGNTAAYCGTGCQQGPC 238
Score = 33.6 bits (67), Expect(3) = 3e-046
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -3 / +2
Query: 683 SNAYCDASNRQWPCAAGQKYY 621
S YCD SN ++PCA + YY
Sbjct: 524 SKDYCDESNTKYPCAPNKGYY 586
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,283,319
Number of Sequences: 7669
Number of extensions: 69334
Number of successful extensions: 570
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 334443564
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)