TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804972.2.1
(1311 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular tr... 206 5e-054
gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular tr... 203 5e-053
emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-red... 117 3e-041
gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mR... 73 5e-037
gb|CO513763.1|CO513763 s13dSG15H0800064_140308 Glandular tr... 144 2e-035
>gb|CO512887.1|CO512887 s13dSG20H0700060_121700 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 602
Score = 206 bits (444), Expect = 5e-054
Identities = 86/122 (70%), Positives = 101/122 (82%)
Frame = +3 / +1
Query: 132 CVTGASGYIASWIVRLLLDRGYTVRATVRDTADPKKTLHLTALDGAKDRLHLFKASLLEE 311
CVTGASGYIASW+VRLLL+RGYTV+ATVRD DPKK HL L+GAK+RL LFKA+LLEE
Sbjct: 73 CVTGASGYIASWLVRLLLNRGYTVKATVRDPNDPKKGDHLVKLEGAKERLQLFKANLLEE 252
Query: 312 GSFDAAVHGCDTVFHTASPFYHNVKDAKAELLDPAVKGTLNVLGSCKKASIKKVVVTSSM 491
G+FD+ V GC VFHTASPFYH+VKD +AEL+DPA+KGTLNVL SC K+ K +V +S
Sbjct: 253 GAFDSVVQGCHGVFHTASPFYHDVKDPQAELIDPALKGTLNVLNSCAKSPSLKRLVLTSS 432
Query: 492 AA 497
A
Sbjct: 433 IA 438
Score = 115 bits (246), Expect(2) = 1e-034
Identities = 42/69 (60%), Positives = 57/69 (82%)
Frame = +3 / +1
Query: 450 KKASIKKVVVTSSMAAVAYNGRPRTPEVTVDETWFSDPQICETNQQWYILSKTLAEEAAW 629
K S+K++V+TSS+AAVAYNG+PRTP+V VDETWF+D +C + WY++SKTLAEEAAW
Sbjct: 394 KSPSLKRLVLTSSIAAVAYNGKPRTPDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAW 573
Query: 630 KFSRDNGLE 656
KF + N ++
Sbjct: 574 KFVKVNNID 600
Score = 101 bits (216), Expect(2) = 2e-031
Identities = 49/80 (61%), Positives = 57/80 (71%)
Frame = -3 / -1
Query: 370 KGEAV*NTVSHP*TAASKEPSSSRLALNKCSLSLAPSNAVRCNVFFGSAVSRTVARTV*P 191
KG+AV* T P*T SK PSSSR ALN C+LSLAPS+ + + F GS SRTVA TV*P
Sbjct: 311 KGDAV*KTP*QP*TTESKAPSSSRFALNSCNLSLAPSSLTKWSPFLGSFGSRTVALTV*P 132
Query: 190 RSRRSLTIQDAMYPDAPVTH 131
R R + T +AM P+APVTH
Sbjct: 131 RLRSNRTSHEAM*PEAPVTH 72
Score = 51.9 bits (107), Expect(2) = 2e-031
Identities = 26/51 (50%), Positives = 31/51 (60%)
Frame = -3 / -1
Query: 658 ISSPLSLENFQAASSASVLDKIYHC*FVSQICGSENHVSSTVTSGVLGLPL 506
IS +L NF AASSA+V + YH ++ S N VSST TSGV G PL
Sbjct: 602 ISMLFTLTNFHAASSANVFETTYHRFDLAHKSASVNQVSSTTTSGVRGFPL 450
Score = 46.4 bits (95), Expect(2) = 1e-034
Identities = 25/61 (40%), Positives = 34/61 (55%)
Frame = +2 / +3
Query: 266 C*G*AALI*S*PARRGFFRCCSSWMRHCISYCLSLLSQCQGC*GRVT*PSS*GNTQCSRF 445
C*G A + RG C S + C+S+C+SLLS CQG G + * S ++QCS+
Sbjct: 207 C*GEIATVQGKST*RGCL*FCCSRLSWCLSHCISLLS*CQGPSG*ID*SCSQRDSQCSQL 386
Query: 446 M 448
M
Sbjct: 387 M 389
>gb|CO512956.1|CO512956 s13dSG86G1100084_121838 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 584
Score = 203 bits (437), Expect = 5e-053
Identities = 85/122 (69%), Positives = 100/122 (81%)
Frame = +3 / +1
Query: 132 CVTGASGYIASWIVRLLLDRGYTVRATVRDTADPKKTLHLTALDGAKDRLHLFKASLLEE 311
CVTGASGYIASW+V LLL+RGYTV+ATVRD DPKK HL L+GAK+RL LFKA+LLEE
Sbjct: 73 CVTGASGYIASWLV*LLLNRGYTVKATVRDPNDPKKVDHLVKLEGAKERLQLFKANLLEE 252
Query: 312 GSFDAAVHGCDTVFHTASPFYHNVKDAKAELLDPAVKGTLNVLGSCKKASIKKVVVTSSM 491
G+FD+ V GC VFHTASPFYH+VKD +AEL+DPA+KGTLNVL SC K+ K +V +S
Sbjct: 253 GAFDSVVQGCHGVFHTASPFYHDVKDPQAELIDPALKGTLNVLNSCAKSPSLKRLVLTSS 432
Query: 492 AA 497
A
Sbjct: 433 IA 438
Score = 112 bits (239), Expect(2) = 1e-033
Identities = 41/62 (66%), Positives = 53/62 (85%)
Frame = +3 / +1
Query: 450 KKASIKKVVVTSSMAAVAYNGRPRTPEVTVDETWFSDPQICETNQQWYILSKTLAEEAAW 629
K S+K++V+TSS+AAVAYNG+PRTP+V VDETWF+D +C + WY++SKTLAEEAAW
Sbjct: 394 KSPSLKRLVLTSSIAAVAYNGKPRTPDVVVDETWFTDADLCAKSNLWYVVSKTLAEEAAW 573
Query: 630 KF 635
KF
Sbjct: 574 KF 579
Score = 102 bits (218), Expect(2) = 6e-031
Identities = 49/80 (61%), Positives = 57/80 (71%)
Frame = -3 / -1
Query: 370 KGEAV*NTVSHP*TAASKEPSSSRLALNKCSLSLAPSNAVRCNVFFGSAVSRTVARTV*P 191
KG+AV* T P*T SK PSSSR ALN C+LSLAPS+ + + F GS SRTVA TV*P
Sbjct: 311 KGDAV*KTP*QP*TTESKAPSSSRFALNSCNLSLAPSSLTKWSTFLGSFGSRTVALTV*P 132
Query: 190 RSRRSLTIQDAMYPDAPVTH 131
R R + T +AM P+APVTH
Sbjct: 131 RLRSNQTSHEAM*PEAPVTH 72
Score = 49.6 bits (102), Expect(2) = 6e-031
Identities = 24/45 (53%), Positives = 28/45 (62%)
Frame = -3 / -1
Query: 640 LENFQAASSASVLDKIYHC*FVSQICGSENHVSSTVTSGVLGLPL 506
L NF AASSA+V + YH ++ S N VSST TSGV G PL
Sbjct: 584 LTNFHAASSANVFETTYHRFDLAHKSASVNQVSSTTTSGVRGFPL 450
Score = 46.4 bits (95), Expect(2) = 1e-033
Identities = 25/61 (40%), Positives = 34/61 (55%)
Frame = +2 / +3
Query: 266 C*G*AALI*S*PARRGFFRCCSSWMRHCISYCLSLLSQCQGC*GRVT*PSS*GNTQCSRF 445
C*G A + RG C S + C+S+C+SLLS CQG G + * S ++QCS+
Sbjct: 207 C*GEIATVQGKST*RGCL*FCCSRLSWCLSHCISLLS*CQGPSG*ID*SCSQRDSQCSQL 386
Query: 446 M 448
M
Sbjct: 387 M 389
>emb|X80222.1|MSDFR1 M.sativa mRNA for dihydroflavonol-4-reductase
Length = 650
Score = 117 bits (250), Expect(2) = 3e-041
Identities = 50/111 (45%), Positives = 71/111 (63%)
Frame = +3 / +1
Query: 162 SWIVRLLLDRGYTVRATVRDTADPKKTLHLTALDGAKDRLHLFKASLLEEGSFDAAVHGC 341
SW+V L++ GY VRATVRD + KK L L GAK +L ++KA L EEGSFD A+ GC
Sbjct: 4 SWLVMRLMEPGYMVRATVRDPENLKKVSPLLELPGAKSKLSIWKADLGEEGSFDEAIKGC 183
Query: 342 DTVFHTASPFYHNVKDAKAELLDPAVKGTLNVLGSCKKASIKKVVVTSSMA 494
VFH A+P KD + E++ P +KG L+++ +C KA + ++ +S A
Sbjct: 184 TGVFHVATPMDFESKDPENEMIKPTIKGVLDIMKACLKAKTVRRLIYTSSA 336
Score = 66.6 bits (139), Expect(2) = 3e-041
Identities = 25/58 (43%), Positives = 40/58 (68%)
Frame = +3 / +1
Query: 591 YILSKTLAEEAAWKFSRDNGLEIVTINPAMVIGPLLQPTLNTSAEAILKLINGSSSTY 764
Y +SKTLAE+ AWKF++++ ++++TI P +V+GP L PT+ S L I G+ + Y
Sbjct: 436 YFVSKTLAEQEAWKFAKEHKMDVITIIPPLVVGPFLIPTMPPSLITALSPITGNEAHY 609
>gb|U28213.1|MSU28213 Medicago sativa vestitone reductase mRNA, complete cds
Length = 1237
Score = 73.0 bits (153), Expect(3) = 5e-037
Identities = 38/109 (34%), Positives = 54/109 (49%)
Frame = +3 / +2
Query: 591 YILSKTLAEEAAWKFSRDNGLEIVTINPAMVIGPLLQPTLNTSAEAILKLINGSSSTYPN 770
Y +SKTLAE+A +F NG+++VT+ ++G + P L S E L L+ G
Sbjct: 542 YAVSKTLAEKAVLEFGEQNGIDVVTLILPFIVGRFVCPKLPDSIEKALVLVLGKKEQIGV 721
Query: 771 FCFGWVNVKDVALAHILAYEVPSSNGRYCMVERVVHYSELVNIIRNMYP 917
F V+V DVA AHI E GRY +V E+ ++ YP
Sbjct: 722 TRFHMVHVDDVARAHIYLLENSVPGGRYNCSPFIVPIEEMSQLLSAKYP 868
Score = 65.2 bits (136), Expect(3) = 5e-037
Identities = 30/80 (37%), Positives = 42/80 (52%)
Frame = +3 / +2
Query: 249 LTALDGAKDRLHLFKASLLEEGSFDAAVHGCDTVFHTASPFYHNVKDAKAELLDPAVKGT 428
LT L GA ++LH F A L SF AA+ GC +FHTASP V + + + V G
Sbjct: 197 LTNLPGASEKLHFFNADLSNPDSFAAAIEGCVGIFHTASPIDFAVSEPEEIVTKRTVDGA 376
Query: 429 LNVLGSCKKASIKKVVVTSS 488
L +L +C + K + +S
Sbjct: 377 LGILKACVNSKTVKRFIYTS 436
Score = 49.6 bits (102), Expect(3) = 5e-037
Identities = 16/36 (44%), Positives = 26/36 (72%)
Frame = +3 / +2
Query: 132 CVTGASGYIASWIVRLLLDRGYTVRATVRDTADPKK 239
CVTG +G++ SWI++ LL+ GY+V T+R + K+
Sbjct: 77 CVTGGTGFLGSWIIKSLLENGYSVNTTIRADPERKR 184
>gb|CO513763.1|CO513763 s13dSG15H0800064_140308 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 485
Score = 144 bits (309), Expect = 2e-035
Identities = 54/153 (35%), Positives = 93/153 (60%)
Frame = +3 / +1
Query: 609 LAEEAAWKFSRDNGLEIVTINPAMVIGPLLQPTLNTSAEAILKLINGSSSTYPNFCFGWV 788
LAE+A W+F+++ G ++V INP +GPL+ P +N+S + ++ G TY +F G
Sbjct: 4 LAEKAGWEFAKETGFDVVMINPGTALGPLIPPRINSSMAVLAGVLKGDKETYEDFFMGMA 183
Query: 789 NVKDVALAHILAYEVPSSNGRYCMVERVVHYSELVNIIRNMYPTLPLPDKCADDKPFVPP 968
+ KD+ALAHIL +E ++GR+ VE + HYS+ VN++ +YP + D +P +
Sbjct: 184 HFKDIALAHILGFEQKKASGRHLCVEAIRHYSDFVNLVAELYPEYNVAKIPTDTQPGLLR 363
Query: 969 YQVSKEKIKSIGIELIPLETSVKETIESLKEKG 1067
+ + +K+ +G+E P E +K+ +E LK +G
Sbjct: 364 AKNASKKLIDLGLEFTPAEQIIKDAVECLKSRG 462
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,917,374
Number of Sequences: 7669
Number of extensions: 153991
Number of successful extensions: 2326
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 357163744
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)