TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804918.2.5
(733 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO515573.1|CO515573 s13dSG47A0200017_418205 Glandular tr... 358 e-100
gb|CO512608.1|CO512608 s13dSG13C0400022_121142 Glandular tr... 356 2e-099
gb|CO514475.1|CO514475 s13dSG43B0600047_327610 Glandular tr... 332 3e-092
gb|CO513390.1|CO513390 s13dSG38E0400023_129944 Glandular tr... 264 6e-072
gb|AJ966562.1|AJ966562 AJ966562 Medicago sativa subsp. falc... 126 4e-030
gb|CO513639.1|CO513639 s13dSG17A0400021_140060 Glandular tr... 113 2e-026
>gb|CO515573.1|CO515573 s13dSG47A0200017_418205 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 572
Score = 358 bits (776), Expect = e-100
Identities = 152/165 (92%), Positives = 161/165 (97%)
Frame = -2 / +3
Query: 726 AAMPPKLDPSQVVEVFVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLR 547
+ MPPKLDPSQ+VEV+VRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLR
Sbjct: 75 STMPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLR 254
Query: 546 VTVKLTVQNRQAKVSVVPSAAALVIKALKEPERDRKKVKNIKHSGNISLDDVVEIARTMR 367
VTVKLTVQNRQAKV+VVPSAAALVIKALKEPERDRKK KNIKH+GNISLDDV+EIA+ M+
Sbjct: 255 VTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMK 434
Query: 366 NRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGEVEI 232
RSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDG+VEI
Sbjct: 435 PRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGDVEI 569
Score = 244 bits (528), Expect(2) = 1e-066
Identities = 111/146 (76%), Positives = 121/146 (82%)
Frame = +2 / -2
Query: 284 PSTVHPTLTQVPRISLTVPANSLAMDLFLMVLAISTTSSRLMLPLCLMFLTFFRSLSGSL 463
PSTVHPT TQVP+IS TVPA+S A+DL ++ AIS TSS + PLCLMFL F RSLSGS
Sbjct: 517 PSTVHPTETQVPKISFTVPASSFAIDLGFIIFAISITSSSEIFPLCLMFLVFLRSLSGSF 338
Query: 464 SALMTSAAAEGTTETLAWRFWTVSLTVTRRPFQSLAVSLAMSSPIFFGESPSGPILGARD 643
AL+TSAAA+GTT TLA RF TVSLTVT RPFQSLAVSLAMSSPIFFGE P GPILGAR+
Sbjct: 337 KALITSAAADGTTATLA*RF*TVSLTVTLRPFQSLAVSLAMSSPIFFGERPRGPILGARE 158
Query: 644 DAAPTSPPVTRTKTSTTCDGSSLGGM 721
AAPTSPPVTRT TST DGSS GG+
Sbjct: 157 LAAPTSPPVTRT*TSTIWDGSSFGGI 80
Score = 23.5 bits (45), Expect(2) = 1e-066
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = +1 / -1
Query: 232 DLDLAVINLLLQVL 273
+L++ +INLLLQ+L
Sbjct: 569 NLNITIINLLLQIL 528
>gb|CO512608.1|CO512608 s13dSG13C0400022_121142 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 563
Score = 356 bits (772), Expect = 2e-099
Identities = 151/164 (92%), Positives = 160/164 (97%)
Frame = -2 / +3
Query: 726 AAMPPKLDPSQVVEVFVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLR 547
+ MPPKLDPSQ+VEV+VRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLR
Sbjct: 72 STMPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLR 251
Query: 546 VTVKLTVQNRQAKVSVVPSAAALVIKALKEPERDRKKVKNIKHSGNISLDDVVEIARTMR 367
VTVKLTVQNRQAKV+VVPSAAALVIKALKEPERDRKK KNIKH+GNISLDDV+EIA+ M+
Sbjct: 252 VTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMK 431
Query: 366 NRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGEVE 235
RSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDG+VE
Sbjct: 432 PRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGDVE 563
Score = 244 bits (528), Expect(2) = 1e-066
Identities = 111/146 (76%), Positives = 121/146 (82%)
Frame = +2 / -2
Query: 284 PSTVHPTLTQVPRISLTVPANSLAMDLFLMVLAISTTSSRLMLPLCLMFLTFFRSLSGSL 463
PSTVHPT TQVP+IS TVPA+S A+DL ++ AIS TSS + PLCLMFL F RSLSGS
Sbjct: 514 PSTVHPTETQVPKISFTVPASSFAIDLGFIIFAISITSSSEIFPLCLMFLVFLRSLSGSF 335
Query: 464 SALMTSAAAEGTTETLAWRFWTVSLTVTRRPFQSLAVSLAMSSPIFFGESPSGPILGARD 643
AL+TSAAA+GTT TLA RF TVSLTVT RPFQSLAVSLAMSSPIFFGE P GPILGAR+
Sbjct: 334 KALITSAAADGTTATLA*RF*TVSLTVTLRPFQSLAVSLAMSSPIFFGERPRGPILGARE 155
Query: 644 DAAPTSPPVTRTKTSTTCDGSSLGGM 721
AAPTSPPVTRT TST DGSS GG+
Sbjct: 154 LAAPTSPPVTRT*TSTIWDGSSFGGI 77
Score = 23.1 bits (44), Expect(2) = 1e-066
Identities = 8/13 (61%), Positives = 12/13 (92%)
Frame = +1 / -1
Query: 235 LDLAVINLLLQVL 273
L++ +INLLLQ+L
Sbjct: 563 LNITIINLLLQIL 525
>gb|CO514475.1|CO514475 s13dSG43B0600047_327610 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 518
Score = 332 bits (719), Expect = 3e-092
Identities = 141/151 (93%), Positives = 148/151 (98%)
Frame = -2 / +3
Query: 720 MPPKLDPSQVVEVFVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVT 541
MPPKLDPSQ+VEV+VRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVT
Sbjct: 66 MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVT 245
Query: 540 VKLTVQNRQAKVSVVPSAAALVIKALKEPERDRKKVKNIKHSGNISLDDVVEIARTMRNR 361
VKLTVQNRQAKV+VVPSAAALVIKALKEPERDRKK KNIKH+GNISLDDV+EIA+ M+ R
Sbjct: 246 VKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPR 425
Query: 360 SMAKELAGTVKEILGTCVSVGCTVDGKDPKD 268
SMAKELAGTVKEILGTCVSVGCTVDGKDPKD
Sbjct: 426 SMAKELAGTVKEILGTCVSVGCTVDGKDPKD 518
Score = 255 bits (551), Expect = 5e-069
Identities = 112/146 (76%), Positives = 122/146 (83%)
Frame = +2 / -2
Query: 284 PSTVHPTLTQVPRISLTVPANSLAMDLFLMVLAISTTSSRLMLPLCLMFLTFFRSLSGSL 463
PSTVHPT TQVPRIS TVPA+S A+DL ++ AIS TSS + PLCL+F FF SLSGS
Sbjct: 502 PSTVHPTETQVPRISFTVPASSFAIDLGFIIFAISITSSSEIFPLCLIFFVFFLSLSGSF 323
Query: 464 SALMTSAAAEGTTETLAWRFWTVSLTVTRRPFQSLAVSLAMSSPIFFGESPSGPILGARD 643
+AL+TSAAAEGTT T A RFWTVSLTVTRRPFQS AVSLAMSSPIFFGE P GPILGAR+
Sbjct: 322 NALITSAAAEGTTATFA*RFWTVSLTVTRRPFQSFAVSLAMSSPIFFGERPRGPILGARE 143
Query: 644 DAAPTSPPVTRTKTSTTCDGSSLGGM 721
AAPTSPPVTRT TST CDGSS GG+
Sbjct: 142 LAAPTSPPVTRT*TSTICDGSSFGGI 65
>gb|CO513390.1|CO513390 s13dSG38E0400023_129944 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 440
Score = 264 bits (572), Expect = 6e-072
Identities = 113/125 (90%), Positives = 121/125 (96%)
Frame = -2 / +2
Query: 726 AAMPPKLDPSQVVEVFVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLR 547
+ MPPKLDPSQ+VEV+VRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLR
Sbjct: 65 STMPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLR 244
Query: 546 VTVKLTVQNRQAKVSVVPSAAALVIKALKEPERDRKKVKNIKHSGNISLDDVVEIARTMR 367
VTVKLTVQNRQAKV+VVPSAAALVIKALKEPERDRKK KNIKH+GNISLDDV+EIA+ M+
Sbjct: 245 VTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMK 424
Query: 366 NRSMA 352
RSMA
Sbjct: 425 PRSMA 439
Score = 202 bits (435), Expect = 5e-053
Identities = 93/123 (75%), Positives = 101/123 (82%)
Frame = +2 / -3
Query: 353 AMDLFLMVLAISTTSSRLMLPLCLMFLTFFRSLSGSLSALMTSAAAEGTTETLAWRFWTV 532
A+DL ++ AIS TSS + PLCLMFL F RSLSGS AL+TSAAA+GTT TLA RF TV
Sbjct: 438 AIDLGFIIFAISITSSSEIFPLCLMFLVFLRSLSGSFKALITSAAADGTTATLA*RF*TV 259
Query: 533 SLTVTRRPFQSLAVSLAMSSPIFFGESPSGPILGARDDAAPTSPPVTRTKTSTTCDGSSL 712
SLTVT RPFQSLAVSLAMSSPIFFGE P GPILGAR+ AAPTSPPVTRT TST DGSS
Sbjct: 258 SLTVTLRPFQSLAVSLAMSSPIFFGERPRGPILGARELAAPTSPPVTRT*TSTIWDGSSF 79
Query: 713 GGM 721
GG+
Sbjct: 78 GGI 70
>gb|AJ966562.1|AJ966562 AJ966562 Medicago sativa subsp. falcata flower buds Medicago sativa
subsp. falcata cDNA clone Em13, mRNA sequence
Length = 280
Score = 126 bits (269), Expect = 4e-030
Identities = 52/65 (80%), Positives = 58/65 (89%)
Frame = -2 / -3
Query: 423 KHSGNISLDDVVEIARTMRNRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDG 244
KH+GNISLDDV+EIA+ M+ RSMAKELAG VKEILGTCVSVG DGKDPK LQQ+IDDG
Sbjct: 278 KHNGNISLDDVIEIAKIMKPRSMAKELAGAVKEILGTCVSVGVLCDGKDPKHLQQQIDDG 99
Query: 243 EVEIP 229
+VEIP
Sbjct: 98 DVEIP 84
>gb|CO513639.1|CO513639 s13dSG17A0400021_140060 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 316
Score = 113 bits (242), Expect = 2e-026
Identities = 47/53 (88%), Positives = 50/53 (94%)
Frame = -2 / +2
Query: 387 EIARTMRNRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGEVEIP 229
EIA+ M+ RSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEI DG+VEIP
Sbjct: 5 EIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEISDGDVEIP 163
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,881,482
Number of Sequences: 7669
Number of extensions: 48180
Number of successful extensions: 750
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 186220470
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)