TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804918.2.4
(634 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO515573.1|CO515573 s13dSG47A0200017_418205 Glandular tr... 326 2e-090
gb|CO512608.1|CO512608 s13dSG13C0400022_121142 Glandular tr... 324 6e-090
gb|CO514475.1|CO514475 s13dSG43B0600047_327610 Glandular tr... 300 9e-083
gb|CO513390.1|CO513390 s13dSG38E0400023_129944 Glandular tr... 232 2e-062
gb|AJ966562.1|AJ966562 AJ966562 Medicago sativa subsp. falc... 127 9e-031
gb|CO513639.1|CO513639 s13dSG17A0400021_140060 Glandular tr... 115 7e-027
>gb|CO515573.1|CO515573 s13dSG47A0200017_418205 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 572
Score = 326 bits (706), Expect = 2e-090
Identities = 141/148 (95%), Positives = 145/148 (97%)
Frame = -1 / +3
Query: 634 RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVSVV 455
RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKV+VV
Sbjct: 126 RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVV 305
Query: 454 PSAAALVIKALKEPERDRKKVKNIKHSGNISLDDVIEIAKTMRNRSMAKELAGTVKEILG 275
PSAAALVIKALKEPERDRKK KNIKH+GNISLDDVIEIAK M+ RSMAKELAGTVKEILG
Sbjct: 306 PSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILG 485
Query: 274 TCVSVGCTVDGKDPKDLQQEIDDGEVEI 191
TCVSVGCTVDGKDPKDLQQEIDDG+VEI
Sbjct: 486 TCVSVGCTVDGKDPKDLQQEIDDGDVEI 569
Score = 180 bits (387), Expect(2) = 8e-049
Identities = 83/131 (63%), Positives = 92/131 (70%)
Frame = +3 / -2
Query: 240 FPSTVHPTLTQVPRISLTVPANSLAMDLFRMVLAISMTSSRLMLPLCXXXXXXXXXXXXX 419
FPSTVHPT TQVP+IS TVPA+S A+DL ++ AIS+TSS + PLC
Sbjct: 520 FPSTVHPTETQVPKISFTVPASSFAIDLGFIIFAISITSSSEIFPLCLMFLVFLRSLSGS 341
Query: 420 XXXXXXXXXXEGTTETLACRFCTVSLTVTRRPFQSLAVSLAMSSPIFFGERPSGPILGAS 599
+GTT TLA RF TVSLTVT RPFQSLAVSLAMSSPIFFGERP GPILGA
Sbjct: 340 FKALITSAAADGTTATLA*RF*TVSLTVTLRPFQSLAVSLAMSSPIFFGERPRGPILGAR 161
Query: 600 DDAAPTSPPVT 632
+ AAPTSPPVT
Sbjct: 160 ELAAPTSPPVT 128
Score = 28.1 bits (55), Expect(2) = 8e-049
Identities = 9/13 (69%), Positives = 12/13 (92%)
Frame = +1 / -3
Query: 193 SRPHHHRSPAASP 231
S+ HHH+SPAA+P
Sbjct: 567 SQHHHHQSPAANP 529
>gb|CO512608.1|CO512608 s13dSG13C0400022_121142 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 563
Score = 324 bits (702), Expect = 6e-090
Identities = 140/147 (95%), Positives = 144/147 (97%)
Frame = -1 / +3
Query: 634 RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVSVV 455
RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKV+VV
Sbjct: 123 RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVV 302
Query: 454 PSAAALVIKALKEPERDRKKVKNIKHSGNISLDDVIEIAKTMRNRSMAKELAGTVKEILG 275
PSAAALVIKALKEPERDRKK KNIKH+GNISLDDVIEIAK M+ RSMAKELAGTVKEILG
Sbjct: 303 PSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILG 482
Query: 274 TCVSVGCTVDGKDPKDLQQEIDDGEVE 194
TCVSVGCTVDGKDPKDLQQEIDDG+VE
Sbjct: 483 TCVSVGCTVDGKDPKDLQQEIDDGDVE 563
Score = 180 bits (387), Expect(2) = 2e-048
Identities = 83/131 (63%), Positives = 92/131 (70%)
Frame = +3 / -2
Query: 240 FPSTVHPTLTQVPRISLTVPANSLAMDLFRMVLAISMTSSRLMLPLCXXXXXXXXXXXXX 419
FPSTVHPT TQVP+IS TVPA+S A+DL ++ AIS+TSS + PLC
Sbjct: 517 FPSTVHPTETQVPKISFTVPASSFAIDLGFIIFAISITSSSEIFPLCLMFLVFLRSLSGS 338
Query: 420 XXXXXXXXXXEGTTETLACRFCTVSLTVTRRPFQSLAVSLAMSSPIFFGERPSGPILGAS 599
+GTT TLA RF TVSLTVT RPFQSLAVSLAMSSPIFFGERP GPILGA
Sbjct: 337 FKALITSAAADGTTATLA*RF*TVSLTVTLRPFQSLAVSLAMSSPIFFGERPRGPILGAR 158
Query: 600 DDAAPTSPPVT 632
+ AAPTSPPVT
Sbjct: 157 ELAAPTSPPVT 125
Score = 26.7 bits (52), Expect(2) = 2e-048
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = +1 / -3
Query: 202 HHHRSPAASP 231
HHH+SPAA+P
Sbjct: 555 HHHQSPAANP 526
>gb|CO514475.1|CO514475 s13dSG43B0600047_327610 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 518
Score = 300 bits (650), Expect = 9e-083
Identities = 130/136 (95%), Positives = 133/136 (97%)
Frame = -1 / +3
Query: 634 RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVSVV 455
RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKV+VV
Sbjct: 111 RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVV 290
Query: 454 PSAAALVIKALKEPERDRKKVKNIKHSGNISLDDVIEIAKTMRNRSMAKELAGTVKEILG 275
PSAAALVIKALKEPERDRKK KNIKH+GNISLDDVIEIAK M+ RSMAKELAGTVKEILG
Sbjct: 291 PSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILG 470
Query: 274 TCVSVGCTVDGKDPKD 227
TCVSVGCTVDGKDPKD
Sbjct: 471 TCVSVGCTVDGKDPKD 518
Score = 183 bits (394), Expect = 2e-047
Identities = 84/131 (64%), Positives = 91/131 (69%)
Frame = +3 / -2
Query: 240 FPSTVHPTLTQVPRISLTVPANSLAMDLFRMVLAISMTSSRLMLPLCXXXXXXXXXXXXX 419
FPSTVHPT TQVPRIS TVPA+S A+DL ++ AIS+TSS + PLC
Sbjct: 505 FPSTVHPTETQVPRISFTVPASSFAIDLGFIIFAISITSSSEIFPLCLIFFVFFLSLSGS 326
Query: 420 XXXXXXXXXXEGTTETLACRFCTVSLTVTRRPFQSLAVSLAMSSPIFFGERPSGPILGAS 599
EGTT T A RF TVSLTVTRRPFQS AVSLAMSSPIFFGERP GPILGA
Sbjct: 325 FNALITSAAAEGTTATFA*RFWTVSLTVTRRPFQSFAVSLAMSSPIFFGERPRGPILGAR 146
Query: 600 DDAAPTSPPVT 632
+ AAPTSPPVT
Sbjct: 145 ELAAPTSPPVT 113
>gb|CO513390.1|CO513390 s13dSG38E0400023_129944 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 440
Score = 232 bits (502), Expect = 2e-062
Identities = 102/108 (94%), Positives = 105/108 (97%)
Frame = -1 / +2
Query: 634 RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVSVV 455
RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKV+VV
Sbjct: 116 RVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVV 295
Query: 454 PSAAALVIKALKEPERDRKKVKNIKHSGNISLDDVIEIAKTMRNRSMA 311
PSAAALVIKALKEPERDRKK KNIKH+GNISLDDVIEIAK M+ RSMA
Sbjct: 296 PSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMA 439
Score = 134 bits (288), Expect = 8e-033
Identities = 64/107 (59%), Positives = 71/107 (66%)
Frame = +3 / -3
Query: 312 AMDLFRMVLAISMTSSRLMLPLCXXXXXXXXXXXXXXXXXXXXXXXEGTTETLACRFCTV 491
A+DL ++ AIS+TSS + PLC +GTT TLA RF TV
Sbjct: 438 AIDLGFIIFAISITSSSEIFPLCLMFLVFLRSLSGSFKALITSAAADGTTATLA*RF*TV 259
Query: 492 SLTVTRRPFQSLAVSLAMSSPIFFGERPSGPILGASDDAAPTSPPVT 632
SLTVT RPFQSLAVSLAMSSPIFFGERP GPILGA + AAPTSPPVT
Sbjct: 258 SLTVTLRPFQSLAVSLAMSSPIFFGERPRGPILGARELAAPTSPPVT 118
>gb|AJ966562.1|AJ966562 AJ966562 Medicago sativa subsp. falcata flower buds Medicago sativa
subsp. falcata cDNA clone Em13, mRNA sequence
Length = 280
Score = 127 bits (273), Expect = 9e-031
Identities = 54/65 (83%), Positives = 58/65 (89%)
Frame = -1 / -3
Query: 382 KHSGNISLDDVIEIAKTMRNRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDG 203
KH+GNISLDDVIEIAK M+ RSMAKELAG VKEILGTCVSVG DGKDPK LQQ+IDDG
Sbjct: 278 KHNGNISLDDVIEIAKIMKPRSMAKELAGAVKEILGTCVSVGVLCDGKDPKHLQQQIDDG 99
Query: 202 EVEIP 188
+VEIP
Sbjct: 98 DVEIP 84
>gb|CO513639.1|CO513639 s13dSG17A0400021_140060 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 316
Score = 115 bits (245), Expect = 7e-027
Identities = 48/53 (90%), Positives = 50/53 (94%)
Frame = -1 / +2
Query: 346 EIAKTMRNRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEIDDGEVEIP 188
EIAK M+ RSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEI DG+VEIP
Sbjct: 5 EIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEISDGDVEIP 163
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,366,650
Number of Sequences: 7669
Number of extensions: 36799
Number of successful extensions: 764
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 155646960
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)