TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1738955.2.1
(1271 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular tr... 268 1e-072
gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular tr... 136 1e-066
gb|CO515743.1|CO515743 s13dSG77C1100086_419635 Glandular tr... 218 1e-057
gb|CO514666.1|CO514666 s13dSG46E1100087_327992 Glandular tr... 110 6e-051
gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular tr... 162 9e-041
>gb|CO513205.1|CO513205 s13dSG24B0800061_129574 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 268 bits (579), Expect = 1e-072
Identities = 102/149 (68%), Positives = 120/149 (80%)
Frame = -1 / +2
Query: 1124 GSWQSAHATFYXXXXXXXXXXXXXGYGNMYSTGYGTNTAALSTALFNDGAACGSCYELRC 945
G W +AHATFY GYGN+YS GYGTNTAALSTALFN G +CGSC+E+RC
Sbjct: 146 GGWVNAHATFYGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGSCFEIRC 325
Query: 944 DNNGQSCLPGTITVTATNFCPPNYGLPSDDGGWCNPPRPHFDMAQPAFLQIAQYRAGIVP 765
+ + CLPG+I VTATNFCPPN LP+++GGWC+PP HFD+AQP FL+IAQY+AGIVP
Sbjct: 326 AGDRKWCLPGSILVTATNFCPPNNALPNNNGGWCSPPLQHFDLAQPVFLRIAQYKAGIVP 505
Query: 764 VAYRRVPCVKKGGIRFTINGHSYFNLVLV 678
V+YRRVPC ++GGIRFTINGHSYFNLVLV
Sbjct: 506 VSYRRVPCRRRGGIRFTINGHSYFNLVLV 592
Score = 155 bits (332), Expect = 2e-038
Identities = 74/123 (60%), Positives = 82/123 (66%)
Frame = +1 / -2
Query: 679 TSTRLK*EWPLMVNLIPPFFTHGTLL*ATGTMPARYCAIWRKAGWAMSKCGRGGLHQPPS 858
T T+LK*E PL+VNLIPP GTLL* TGT+PA Y AI RK G A SKC +GGLH PP
Sbjct: 591 TRTKLK*ECPLIVNLIPPLLLQGTLL*ETGTIPALY*AILRKTG*ARSKCCKGGLHHPPL 412
Query: 859 SLGRP*LGGQKLVAVTVMVPGRHDCPLLSQRSS*QDPHAAPSLNSAVLSAAVLVPYPVLY 1038
LG+ GGQKLVAVT + PGRH + S Q+P P LN AVL AAV VP P LY
Sbjct: 411 LLGKALFGGQKLVAVTKIEPGRHHLRSPAHLISKQEPQLKPLLNKAVLKAAVFVP*P*LY 232
Query: 1039 MFP 1047
FP
Sbjct: 231 RFP 223
>gb|CO514640.1|CO514640 s13dSG46C0600050_327940 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 536
Score = 136 bits (291), Expect(2) = 1e-066
Identities = 52/65 (80%), Positives = 57/65 (87%)
Frame = -1 / +3
Query: 854 GGWCNPPRPHFDMAQPAFLQIAQYRAGIVPVAYRRVPCVKKGGIRFTINGHSYFNLVLVT 675
GGWC+PP HFD++QP F IAQYRAGIVPVAYRRV C ++GGIRFTINGHSYFNLVLVT
Sbjct: 342 GGWCDPPNHHFDLSQPIFQHIAQYRAGIVPVAYRRVRCKRRGGIRFTINGHSYFNLVLVT 521
Query: 674 NVAGA 660
NV GA
Sbjct: 522 NVGGA 536
Score = 132 bits (283), Expect(2) = 1e-066
Identities = 51/83 (61%), Positives = 61/83 (73%)
Frame = -1 / +3
Query: 1127 YGSWQSAHATFYXXXXXXXXXXXXXGYGNMYSTGYGTNTAALSTALFNDGAACGSCYELR 948
+ SW +AHATFY GYGN+YS GYGTNTAALSTALFN+G +CG+CYE++
Sbjct: 93 HASWINAHATFYGGSDASGTMGGACGYGNLYSQGYGTNTAALSTALFNNGLSCGACYEIK 272
Query: 947 CDNNGQSCLPGTITVTATNFCPP 879
C N+ Q C PG+I VTATNFCPP
Sbjct: 273 CVNDPQWCNPGSIIVTATNFCPP 341
Score = 86.3 bits (182), Expect(2) = 8e-032
Identities = 42/65 (64%), Positives = 45/65 (69%)
Frame = +1 / -2
Query: 661 APATLVTSTRLK*EWPLMVNLIPPFFTHGTLL*ATGTMPARYCAIWRKAGWAMSKCGRGG 840
AP T VT T+LK*E PLMVNLIPP H TLL*ATGT+PA Y A+ K G SK GG
Sbjct: 535 APPTFVTRTKLK*ECPLMVNLIPPLLLHLTLL*ATGTIPALY*AMC*KIG*ERSKW*LGG 356
Query: 841 LHQPP 855
HQPP
Sbjct: 355 SHQPP 341
Score = 66.1 bits (138), Expect(2) = 8e-032
Identities = 32/56 (57%), Positives = 36/56 (64%)
Frame = +1 / -2
Query: 880 GGQKLVAVTVMVPGRHDCPLLSQRSS*QDPHAAPSLNSAVLSAAVLVPYPVLYMFP 1047
GGQKLVAVT++ PG H C + S* P P LNSAVL+AAV VP P L FP
Sbjct: 340 GGQKLVAVTIIDPGLHHCGSFTHLIS*HAPQLNPLLNSAVLNAAVFVP*PWL*RFP 173
>gb|CO515743.1|CO515743 s13dSG77C1100086_419635 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 538
Score = 218 bits (471), Expect = 1e-057
Identities = 82/122 (67%), Positives = 97/122 (79%)
Frame = -1 / +1
Query: 1130 DYGSWQSAHATFYXXXXXXXXXXXXXGYGNMYSTGYGTNTAALSTALFNDGAACGSCYEL 951
DYG W+SAHATFY GYGN+YS GYGTNTAALSTALFN G +CGSCYE+
Sbjct: 172 DYGGWESAHATFYGGGDASGTMGGACGYGNLYSQGYGTNTAALSTALFNSGLSCGSCYEM 351
Query: 950 RCDNNGQSCLPGTITVTATNFCPPNYGLPSDDGGWCNPPRPHFDMAQPAFLQIAQYRAGI 771
RC+++ + C PG+I VTATNFCPPN LP+++GGWCNPP HFDMA+PA+LQIA+YRAGI
Sbjct: 352 RCNDDPRWCKPGSIIVTATNFCPPNPSLPNNNGGWCNPPLQHFDMAEPAYLQIAEYRAGI 531
Query: 770 VP 765
VP
Sbjct: 532 VP 537
Score = 124 bits (266), Expect = 2e-029
Identities = 58/94 (61%), Positives = 64/94 (68%)
Frame = +1 / -3
Query: 766 GTMPARYCAIWRKAGWAMSKCGRGGLHQPPSSLGRP*LGGQKLVAVTVMVPGRHDCPLLS 945
GT+PA Y AI R AG A+SKC +GGLHQPP LG+ GGQK VAVT++ PG H
Sbjct: 536 GTIPALYSAI*R*AGSAISKCCKGGLHQPPLLLGKDGFGGQKFVAVTIIEPGLHHLGSSL 357
Query: 946 QRSS*QDPHAAPSLNSAVLSAAVLVPYPVLYMFP 1047
S*QDP P LN AVLSAAVLVPYP LY FP
Sbjct: 356 HLIS*QDPQLKPLLNKAVLSAAVLVPYPWLYKFP 255
>gb|CO514666.1|CO514666 s13dSG46E1100087_327992 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 542
Score = 110 bits (234), Expect(2) = 6e-051
Identities = 39/51 (76%), Positives = 45/51 (88%)
Frame = -1 / +1
Query: 914 TITVTATNFCPPNYGLPSDDGGWCNPPRPHFDMAQPAFLQIAQYRAGIVPV 762
+I +TATNFCPPN+ PSD+GGWCNPPRPH D+A P FL+IAQYRAGIVPV
Sbjct: 388 SILITATNFCPPNFAQPSDNGGWCNPPRPHSDLAMPMFLKIAQYRAGIVPV 540
Score = 106 bits (226), Expect(2) = 6e-051
Identities = 41/77 (53%), Positives = 51/77 (66%)
Frame = -1 / +1
Query: 1124 GSWQSAHATFYXXXXXXXXXXXXXGYGNMYSTGYGTNTAALSTALFNDGAACGSCYELRC 945
G W SAHATFY GYGN+YS GYG NTAALSTALFN+G +CG+C+EL+C
Sbjct: 172 GPWTSAHATFYGGSDASGTMGGACGYGNLYSQGYGVNTAALSTALFNNGLSCGACFELKC 351
Query: 944 DNNGQSCLPGTITVTAT 894
D + + C PG ++ T
Sbjct: 352 DQDPRWCNPGNPSILIT 402
>gb|CO515120.1|CO515120 s13dSG40E0200019_399105 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 314
Score = 162 bits (348), Expect = 9e-041
Identities = 65/90 (72%), Positives = 71/90 (78%)
Frame = -1 / +3
Query: 719 FTINGHSYFNLVLVTNVAGAGDVQSVSIKGSSTGWQPMSRNWGQNWQSNSLLDGQSLSFQ 540
FTINGHSYFNLVLVTNV GAGDV SV+IKGS T WQ MSRNWGQNWQSNS L+GQSLS
Sbjct: 6 FTINGHSYFNLVLVTNVGGAGDVHSVAIKGSRTRWQAMSRNWGQNWQSNSYLNGQSLSVV 185
Query: 539 VTASDGRTVTSNGVAPAGWQFGQTFEGAQF 450
VT +G ++ S VAP W FGQT+ G QF
Sbjct: 186 VTTGNGHSIVSFNVAPPSWSFGQTYTGRQF 275
Score = 105 bits (225), Expect = 9e-024
Identities = 52/90 (57%), Positives = 58/90 (64%)
Frame = +1 / -2
Query: 451 NWAPSKVWPNCQPAGATPLLVTVRPSLAVTWKDRLWPSRSELLCQFWPQLRDMGCHPVLE 630
N P V P Q GAT + P VT +RL P + ELLCQF PQ DM CH VLE
Sbjct: 274 NCLPV*VCPKDQLGGATLNDTIL*PLPVVTTTERLCPFK*ELLCQFCPQFLDMACHLVLE 95
Query: 631 PLMDTDCTSPAPATLVTSTRLK*EWPLMVN 720
PLM T+CTSPAP TLVT T+LK*E PL+VN
Sbjct: 94 PLMATECTSPAPPTLVTRTKLK*E*PLIVN 5
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,266,799
Number of Sequences: 7669
Number of extensions: 114877
Number of successful extensions: 868
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 349144380
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)