TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1738875.2.1
(761 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U01020.1|U01020 Medicago sativa clone MsCHS6-4 chalcone ... 286 3e-078
gb|L02905.1|ALFCHS9A Medicago sativa chalcone synthase (CHS... 285 6e-078
gb|U01019.1|U01019 Medicago sativa clone MsCHS4-2 chalcone ... 283 1e-077
emb|X68106.1|MSCHSI M.sativa msCHSI mRNA for chalcone synthase 283 1e-077
emb|X68107.1|MSCHSII M.sativa msCHSII mRNA for chalcone syn... 283 1e-077
gb|L02901.1|ALFCHS1A Medicago sativa chalcone synthase (CHS... 283 2e-077
gb|L02903.1|ALFCHS4A Medicago sativa chalcone synthase (CHS... 282 3e-077
gb|U01021.1|U01021 Medicago sativa clone MsCHS12-1 chalcone... 282 3e-077
gb|U01018.1|U01018 Medicago sativa clone MsCHS4-1 chalcone ... 281 7e-077
gb|L02902.1|ALFCHS2A Medicago sativa chalcone synthase (CHS... 277 9e-076
gb|L02904.1|ALFCHS8A Medicago sativa chalcone synthase (CHS... 274 1e-074
gb|DQ122840.1| Medicago sativa clone K9 chalcone synthase m... 265 4e-072
gb|CO514718.1|CO514718 s13dSG55C0800066_328096 Glandular tr... 232 4e-062
gb|CO514656.1|CO514656 s13dSG46D1100094_327972 Glandular tr... 124 1e-029
gb|CO514834.1|CO514834 s13dSG59G1200100_328328 Glandular tr... 39 7e-004
gb|CO516112.1|CO516112 s13dSG65D1200102_445552 Glandular tr... 36 0.006
gb|CO516656.1|CO516656 s13dSG66G0900070_446640 Glandular tr... 36 0.006
gb|CO517196.1|CO517196 s13dSG33G1100088_474476 Glandular tr... 36 0.006
gb|CO515474.1|CO515474 s13dSG52A0100005_418007 Glandular tr... 35 0.012
gb|U89923.1|MSU89923 Medicago sativa asparagine synthetase ... 32 0.11
gb|L40327.1|ALFASPSYN Medicago sativa asparagine synthetase... 31 0.15
gb|DR025668.2|DR025668 EST12 Medicago sativa cDNA-AFLP (Zou... 29 0.99
gb|AY639647.1| Medicago sativa GMPase (GMP) mRNA, complete cds 29 0.99
gb|CO516793.1|CO516793 s13dSG94E0900069_446914 Glandular tr... 28 1.9
gb|CO511922.1|CO511922 s13dSG05F0200027_103744 Glandular tr... 27 3.5
gb|CO515174.1|CO515174 s13dSG62C0600050_399213 Glandular tr... 27 3.5
gb|CO516473.1|CO516473 s13dSG28C1200098_446274 Glandular tr... 27 3.5
gb|CO516916.1|CO516916 s13dSG58D0800064_468378 Glandular tr... 27 3.5
gb|CO512060.1|CO512060 s13dSG18D0800062_108440 Glandular tr... 26 4.8
gb|CO516827.1|CO516827 s13dSG98C0200006_446982 Glandular tr... 26 4.8
gb|CO516836.1|CO516836 s13dSG98D0200014_447000 Glandular tr... 26 4.8
gb|L01660.1|ALFNADHSYN Medicago sativa NADH-glutamate synth... 26 5.5
gb|L37606.1|ALFNDGS Medicago sativa (clone GG16-1) NADH-dep... 26 6.6
gb|CO513867.1|CO513867 s13dSG73E0700051_156396 Glandular tr... 25 9.1
gb|L36120.1|ALFMSA3S Medicago sativa proline-rich protein m... 25 9.1
gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRN... 25 9.1
gb|AF417491.1| Medicago sativa RING-H2 protein (RH2-1) gene... 25 9.1
emb|X88845.1|MSNUM1GEN M.sativa mRNA for nuM1 protein 25 9.1
>gb|U01020.1|U01020 Medicago sativa clone MsCHS6-4 chalcone synthase mRNA, partial cds
Length = 1009
Score = 286 bits (618), Expect = 3e-078
Identities = 115/140 (82%), Positives = 128/140 (91%)
Frame = +2 / +1
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PL ISD+NSIFW+AHPGGP
Sbjct: 439 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNIDKALVEAFQPLNISDYNSIFWIAHPGGP 618
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+GL +M+ATR VLSEYGNMSSACVLFILDEMRK+SA+ G TTGEGLDW
Sbjct: 619 AILDQVEQKLGLKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQQGLKTTGEGLDW 798
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVLHSV
Sbjct: 799 GVLFGFGPGLTIETVVLHSV 858
Score = 177 bits (380), Expect = 2e-045
Identities = 86/140 (61%), Positives = 94/140 (67%)
Frame = -3 / -3
Query: 423 TLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERTWR 244
TLW TTVS VSPGPKP TPQS PSPVV P A+ RISS++ T ALDMLPYS T
Sbjct: 857 TLWRTTVSMVSPGPKPNNTPQSSPSPVVLSPC*ADFFRISSKINNTHALDMLPYSLSTSL 678
Query: 243 VARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTSLS 64
VA I + P ST SR+AGPPG A QK+E SEM SG AS++A MF E PGTSL
Sbjct: 677 VAFIFSGFKPNFCST*SRIAGPPG*AIQKIEL*SEMFSGWKASTNALSMFFETIPGTSLR 498
Query: 63 RWKVSPTSRRWPSMAPSESG 4
R V P SRR PSMAPS SG
Sbjct: 497 R*NVRPASRR*PSMAPSLSG 438
>gb|L02905.1|ALFCHS9A Medicago sativa chalcone synthase (CHS9) mRNA, complete cds
Length = 1405
Score = 285 bits (616), Expect = 6e-078
Identities = 115/140 (82%), Positives = 128/140 (91%)
Frame = +2 / +2
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PL ISD+NSIFW+AHPGGP
Sbjct: 857 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNIDKALVEAFQPLNISDYNSIFWIAHPGGP 1036
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+GL +M+ATR VLSEYGNMSSACVLFILDEMRK+SA+ G TTGEGLDW
Sbjct: 1037 AILDQVEQKLGLKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQAGLKTTGEGLDW 1216
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVLHSV
Sbjct: 1217 GVLFGFGPGLTIETVVLHSV 1276
Score = 178 bits (383), Expect = 8e-046
Identities = 84/142 (59%), Positives = 97/142 (68%)
Frame = -3 / -2
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERT 250
+ TLW TTVS VSPGPKP TPQSRPSPVV P+ A+ RISS++ +T ALD+ PYS T
Sbjct: 1281 IATLWRTTVSMVSPGPKPNNTPQSRPSPVVLSPACADFFRISSKINKTHALDIFPYSLST 1102
Query: 249 WRVARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTS 70
VA I + P ST SR+AGPPG A QK+E SEM +G AS++A MF E PGTS
Sbjct: 1101 SLVAFIFSGFKPNFCST*SRIAGPPG*AIQKIEL*SEMFNGWKASTNALSMFFETIPGTS 922
Query: 69 LSRWKVSPTSRRWPSMAPSESG 4
L R V P SRR PSMAPS SG
Sbjct: 921 LRR*NVRPASRR*PSMAPSLSG 856
>gb|U01019.1|U01019 Medicago sativa clone MsCHS4-2 chalcone synthase mRNA, partial cds
Length = 1268
Score = 283 bits (613), Expect = 1e-077
Identities = 114/140 (81%), Positives = 128/140 (91%)
Frame = +2 / +1
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PLGISD+NSIFW+AHPGGP
Sbjct: 694 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNINKALVEAFEPLGISDYNSIFWIAHPGGP 873
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+ L +M+ATR VLSEYGNMSSACVLFILDEMRK+SA+DG TTGEGL+W
Sbjct: 874 AILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQDGLKTTGEGLEW 1053
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVL SV
Sbjct: 1054 GVLFGFGPGLTIETVVLRSV 1113
Score = 165 bits (354), Expect = 8e-042
Identities = 79/142 (55%), Positives = 93/142 (65%)
Frame = -3 / -1
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERT 250
+ TL TTVS V PGP P +TP S PSPVV PS A+ ISS++ +T ALD+ PYS T
Sbjct: 1118 IATLRKTTVSMVRPGPNPNKTPHSSPSPVVFSPS*ADFFLISSKINKTHALDIFPYSLST 939
Query: 249 WRVARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTS 70
VA I + ST SR+AGPPGCA QK+E SE+P+G AS++A MF E PGTS
Sbjct: 938 SLVAFIFSGFKANFCST*SRIAGPPGCAIQKIEL*SEIPNGSKASTNALLMFFETIPGTS 759
Query: 69 LSRWKVSPTSRRWPSMAPSESG 4
R VSP SRR PSMAPS SG
Sbjct: 758 FRR*NVSPASRR*PSMAPSLSG 693
Score = 43.7 bits (89), Expect(2) = 1e-007
Identities = 15/61 (24%), Positives = 39/61 (63%)
Frame = -2 / -3
Query: 415 EHDGLDGESRAEAEEDAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAVLGEDVAGGA 236
+++ +G++R++ + + P+++ +RG S+L L+H + D+++ H+++ E +GG
Sbjct: 1104 QNNSFNGKTRSKPK*NTPLKSFSRGLQSILS*LLSHFI*DKQNTCT*HISIFTEHFSGGF 925
Query: 235 H 233
H
Sbjct: 924 H 922
Score = 26.3 bits (51), Expect(2) = 1e-007
Identities = 14/66 (21%), Positives = 35/66 (53%)
Frame = -2 / -3
Query: 205 LHLVEDGGPAGVRHPEDGVPVGDAERLERVVQRAADVLGDEPGHVLEQVEGEPHLAEVAV 26
L+L+ + V +P+D + + +++ + + Q DVL + PG++ +V+ +V +
Sbjct: 894 LYLI*NCRSTRVCNPKD*IVIRNSQWFKSLNQCFIDVL*NNPGNIF*KVKC*SSFTKVTI 715
Query: 25 DGALRV 8
+G +
Sbjct: 714 NGPFTI 697
>emb|X68106.1|MSCHSI M.sativa msCHSI mRNA for chalcone synthase
Length = 1361
Score = 283 bits (613), Expect = 1e-077
Identities = 114/140 (81%), Positives = 128/140 (91%)
Frame = +2 / +2
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PLGISD+NSIFW+AHPGGP
Sbjct: 812 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNINKALVEAFEPLGISDYNSIFWIAHPGGP 991
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+ L +M+ATR VLSEYGNMSSACVLFILDEMRK+SA+DG TTGEGL+W
Sbjct: 992 AILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQDGLKTTGEGLEW 1171
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVL SV
Sbjct: 1172 GVLFGFGPGLTIETVVLRSV 1231
Score = 167 bits (359), Expect = 2e-042
Identities = 80/142 (56%), Positives = 94/142 (66%)
Frame = -3 / -3
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERT 250
+ TL TTVS V PGP P +TP S PSPVV PS A+ ISS++ +TQALD+ PYS T
Sbjct: 1236 IATLRKTTVSMVRPGPNPNKTPHSSPSPVVFSPS*ADFFLISSKINKTQALDIFPYSLST 1057
Query: 249 WRVARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTS 70
VA I + ST SR+AGPPGCA QK+E SE+P+G AS++A MF E PGTS
Sbjct: 1056 SLVAFIFSGFKANFCST*SRIAGPPGCAIQKIEL*SEIPNGSKASTNALLMFFETIPGTS 877
Query: 69 LSRWKVSPTSRRWPSMAPSESG 4
R VSP SRR PSMAPS SG
Sbjct: 876 FRR*NVSPASRR*PSMAPSLSG 811
Score = 49.6 bits (102), Expect(2) = 1e-009
Identities = 16/61 (26%), Positives = 40/61 (65%)
Frame = -2 / -2
Query: 415 EHDGLDGESRAEAEEDAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAVLGEDVAGGA 236
+++ +G++R++ + + P+++ +RG S+L G L+H + D+++ H+++ E +GG
Sbjct: 1222 QNNSFNGKTRSKPK*NTPLKSFSRGLQSILSGLLSHFI*DKQNTST*HISIFTEHFSGGF 1043
Query: 235 H 233
H
Sbjct: 1042 H 1040
Score = 27.2 bits (53), Expect(2) = 1e-009
Identities = 14/66 (21%), Positives = 36/66 (54%)
Frame = -2 / -2
Query: 205 LHLVEDGGPAGVRHPEDGVPVGDAERLERVVQRAADVLGDEPGHVLEQVEGEPHLAEVAV 26
L+L+ + V +P+D + + +++ + + Q DVL + PG++ +V+ +V +
Sbjct: 1012 LYLI*NCRSTRVCNPKD*IVIRNSQWFKSLNQCFIDVL*NNPGNIF*KVKC*SSFTKVTI 833
Query: 25 DGALRV 8
+G+ +
Sbjct: 832 NGSFTI 815
>emb|X68107.1|MSCHSII M.sativa msCHSII mRNA for chalcone synthase
Length = 932
Score = 283 bits (613), Expect = 1e-077
Identities = 114/140 (81%), Positives = 128/140 (91%)
Frame = +2 / +2
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PLGISD+NSIFW+AHPGGP
Sbjct: 371 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNINKALVEAFEPLGISDYNSIFWIAHPGGP 550
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+ L +M+ATR VLSEYGNMSSACVLFILDEMRK+SA+DG TTGEGL+W
Sbjct: 551 AILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQDGLKTTGEGLEW 730
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVL SV
Sbjct: 731 GVLFGFGPGLTIETVVLRSV 790
Score = 171 bits (367), Expect = 1e-043
Identities = 82/140 (58%), Positives = 95/140 (67%)
Frame = -3 / -3
Query: 423 TLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERTWR 244
TL TTVS VSPGPKP +TP S PSPVV PS A+ ISS++ +T ALD+ PYS T
Sbjct: 789 TLRKTTVSMVSPGPKPNKTPHSSPSPVVFSPS*ADFFLISSKINKTHALDIFPYSLSTSL 610
Query: 243 VARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTSLS 64
VA I + ST SR+AGPPGCA QK+E SE+P+G AS++A MF E PGTSL
Sbjct: 609 VAFIFSGFKANFCST*SRIAGPPGCAIQKIEL*SEIPNGSKASTNALLMFFETIPGTSLR 430
Query: 63 RWKVSPTSRRWPSMAPSESG 4
R VSP SRR PSMAPS SG
Sbjct: 429 R*NVSPASRR*PSMAPSLSG 370
Score = 44.1 bits (90), Expect(2) = 2e-007
Identities = 15/61 (24%), Positives = 39/61 (63%)
Frame = -2 / -2
Query: 415 EHDGLDGESRAEAEEDAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAVLGEDVAGGA 236
+++ +G+ R++A+ + P+++ +RG S+L L+H + D+++ H+++ + +GG
Sbjct: 781 QNNSFNGKPRSKAK*NTPLKSFSRGLQSILSRLLSHFI*DKQNTCT*HISIFTKHFSGGF 602
Query: 235 H 233
H
Sbjct: 601 H 599
Score = 24.4 bits (47), Expect(2) = 2e-007
Identities = 13/66 (19%), Positives = 35/66 (53%)
Frame = -2 / -2
Query: 205 LHLVEDGGPAGVRHPEDGVPVGDAERLERVVQRAADVLGDEPGHVLEQVEGEPHLAEVAV 26
L+L+ + V +P+D + + +++ + + Q DVL + G++ ++V+ +V +
Sbjct: 571 LNLI*NCRSTRVCNPKD*IVIRNSQWFKSLNQCFIDVL*NNTGNIFKKVKC*SSFTKVTI 392
Query: 25 DGALRV 8
+G +
Sbjct: 391 NGPFTI 374
>gb|L02901.1|ALFCHS1A Medicago sativa chalcone synthase (CHS1) mRNA, complete cds
Length = 1386
Score = 283 bits (612), Expect = 2e-077
Identities = 114/140 (81%), Positives = 127/140 (90%)
Frame = +2 / +2
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PL ISD+NSIFW+AHPGGP
Sbjct: 827 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNIDKALIEAFQPLNISDYNSIFWIAHPGGP 1006
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+GL +M+ATR VLSEYGNMSSACVLFILDEMRK+S + G TTGEGLDW
Sbjct: 1007 AILDQVEEKLGLKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSVQAGLKTTGEGLDW 1186
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVLHSV
Sbjct: 1187 GVLFGFGPGLTIETVVLHSV 1246
Score = 184 bits (397), Expect = 9e-048
Identities = 86/142 (60%), Positives = 97/142 (68%)
Frame = -3 / -1
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERT 250
+ TLW TTVS VSPGPKP TPQSRPSPVV P+ + ISS+M +T ALDMLPYS T
Sbjct: 1251 IATLWRTTVSMVSPGPKPNNTPQSRPSPVVLSPACTDFFLISSKMNKTHALDMLPYSLST 1072
Query: 249 WRVARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTS 70
VA I + P +STWSR+AGPPG A QK+E SEM +G AS +A MF E PGTS
Sbjct: 1071 SLVAFIFSGFKPNFSSTWSRIAGPPG*AIQKIEL*SEMFNGWKASINALSMFFETIPGTS 892
Query: 69 LSRWKVSPTSRRWPSMAPSESG 4
L R V P SRR PSMAPS SG
Sbjct: 891 LRR*NVRPASRR*PSMAPSLSG 826
Score = 51.0 bits (105), Expect = 2e-007
Identities = 23/63 (36%), Positives = 39/63 (61%)
Frame = -2 / -3
Query: 421 AVEHDGLDGESRAEAEEDAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAVLGEDVAG 242
A+E++G +G+SR+EA++ PV+A + G S L L+HL+ DE++ HV + +
Sbjct: 1243 AMENNGFNGKSRSEAKQHTPVKAFSCGFKSRLYRFLSHLI*DEQNTCT*HVTIFTKYFPS 1064
Query: 241 GAH 233
G H
Sbjct: 1063 GLH 1055
>gb|L02903.1|ALFCHS4A Medicago sativa chalcone synthase (CHS4) mRNA, complete cds
Length = 1281
Score = 282 bits (611), Expect = 3e-077
Identities = 114/140 (81%), Positives = 127/140 (90%)
Frame = +2 / +3
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PLGISD+NSIFW+AHPGGP
Sbjct: 726 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNIDKALVEAFQPLGISDYNSIFWIAHPGGP 905
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+ L +MRATR VLSEYGNMSSACVLFILDEMRK+S +DG TTGEGL+W
Sbjct: 906 AILDQVEQKLALKPEKMRATREVLSEYGNMSSACVLFILDEMRKKSTQDGLKTTGEGLEW 1085
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVL SV
Sbjct: 1086 GVLFGFGPGLTIETVVLRSV 1145
Score = 175 bits (377), Expect = 5e-045
Identities = 81/142 (57%), Positives = 95/142 (66%)
Frame = -3 / -3
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERT 250
+ TL TTVS VSPGPKP TP S PSPVV PS + ISS++ +T ALD+ PYS T
Sbjct: 1150 IATLRKTTVSMVSPGPKPNNTPHSSPSPVVFSPS*VDFFLISSKINKTHALDIFPYSLST 971
Query: 249 WRVARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTS 70
VA I + ST S++AGPPGCA QK+E SE+P+G AS++A MF E PGTS
Sbjct: 970 SLVALIFSGFKANFCST*SKIAGPPGCAIQKIEL*SEIPNGWKASTNALSMFFETIPGTS 791
Query: 69 LSRWKVSPTSRRWPSMAPSESG 4
L RW VSP SRR PSMAPS SG
Sbjct: 790 LRRWNVSPASRR*PSMAPSLSG 725
Score = 46.4 bits (95), Expect = 4e-006
Identities = 29/136 (21%), Positives = 72/136 (52%)
Frame = -2 / -2
Query: 415 EHDGLDGESRAEAEEDAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAVLGEDVAGGA 236
+++ G+S ++A+ P ++ + S+L L+H + D+++ H+++ + + G+
Sbjct: 1136 QNNSFYGKSWSKAK*HTPFKSFSCCLQSILS*FLSHFI*DKQNTCT*HISIFTKHFSSGS 957
Query: 235 HPGLVQPDLGLHLVEDGGPAGVRHPEDGVPVGDAERLERVVQRAADVLGDEPGHVLEQVE 56
H +Q L L+L+ + V +P+D V + +++ LE +VL + P ++ ++VE
Sbjct: 956 HLFRLQG*LLLYLI*NCRATRVCNPKD*VVIRNSQWLESFN*CFINVL*NNPRNIFKKVE 777
Query: 55 GEPHLAEVAVDGALRV 8
+V ++G+ +
Sbjct: 776 C*SSFTKVTINGSFTI 729
>gb|U01021.1|U01021 Medicago sativa clone MsCHS12-1 chalcone synthase mRNA, complete cds
Length = 1380
Score = 282 bits (611), Expect = 3e-077
Identities = 114/140 (81%), Positives = 127/140 (90%)
Frame = +2 / +1
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PLGISD+NSIFW+AHPGGP
Sbjct: 808 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNIDKALVEAFQPLGISDYNSIFWIAHPGGP 987
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+ L +MRATR VLSEYGNMSSACVLFILDEMRK+S +DG TTGEGL+W
Sbjct: 988 AILDQVEQKLALKPEKMRATREVLSEYGNMSSACVLFILDEMRKKSTQDGLKTTGEGLEW 1167
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVL SV
Sbjct: 1168 GVLFGFGPGLTIETVVLRSV 1227
Score = 175 bits (377), Expect = 5e-045
Identities = 81/142 (57%), Positives = 95/142 (66%)
Frame = -3 / -2
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERT 250
+ TL TTVS VSPGPKP TP S PSPVV PS + ISS++ +T ALD+ PYS T
Sbjct: 1232 IATLRKTTVSMVSPGPKPNNTPHSSPSPVVFSPS*VDFFLISSKINKTHALDIFPYSLST 1053
Query: 249 WRVARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTS 70
VA I + ST S++AGPPGCA QK+E SE+P+G AS++A MF E PGTS
Sbjct: 1052 SLVALIFSGFKANFCST*SKIAGPPGCAIQKIEL*SEIPNGWKASTNALSMFFETIPGTS 873
Query: 69 LSRWKVSPTSRRWPSMAPSESG 4
L RW VSP SRR PSMAPS SG
Sbjct: 872 LRRWNVSPASRR*PSMAPSLSG 807
Score = 46.4 bits (95), Expect = 4e-006
Identities = 29/136 (21%), Positives = 72/136 (52%)
Frame = -2 / -1
Query: 415 EHDGLDGESRAEAEEDAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAVLGEDVAGGA 236
+++ G+S ++A+ P ++ + S+L L+H + D+++ H+++ + + G+
Sbjct: 1218 QNNSFYGKSWSKAK*HTPFKSFSCCLQSILS*FLSHFI*DKQNTCT*HISIFTKHFSSGS 1039
Query: 235 HPGLVQPDLGLHLVEDGGPAGVRHPEDGVPVGDAERLERVVQRAADVLGDEPGHVLEQVE 56
H +Q L L+L+ + V +P+D V + +++ LE +VL + P ++ ++VE
Sbjct: 1038 HLFRLQG*LLLYLI*NCRATRVCNPKD*VVIRNSQWLESFN*CFINVL*NNPRNIFKKVE 859
Query: 55 GEPHLAEVAVDGALRV 8
+V ++G+ +
Sbjct: 858 C*SSFTKVTINGSFTI 811
>gb|U01018.1|U01018 Medicago sativa clone MsCHS4-1 chalcone synthase mRNA, complete cds
Length = 1423
Score = 281 bits (608), Expect = 7e-077
Identities = 114/140 (81%), Positives = 127/140 (90%)
Frame = +2 / +1
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHL E GLTFHLLKDVPG++SKNI +AL +AF+PL ISD+NSIFW+AHPGGP
Sbjct: 847 PDSEGAIDGHLVEAGLTFHLLKDVPGIVSKNIDKALIEAFQPLNISDYNSIFWIAHPGGP 1026
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+GL +M+ATR VLSEYGNMSSACVLFILDEMRK+SA+ G TTGEGLDW
Sbjct: 1027 AILDQVEEKLGLKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSAQAGLKTTGEGLDW 1206
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVLHSV
Sbjct: 1207 GVLFGFGPGLTIETVVLHSV 1266
Score = 182 bits (393), Expect = 3e-047
Identities = 86/142 (60%), Positives = 96/142 (67%)
Frame = -3 / -3
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERT 250
+ TLW TTVS VSPGPKP TPQSRPSPVV P+ A+ ISS+M T ALDMLPYS T
Sbjct: 1271 IATLWRTTVSMVSPGPKPNNTPQSRPSPVVLSPACADFFLISSKMNNTHALDMLPYSLST 1092
Query: 249 WRVARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTS 70
VA I + P +STWSR+AGPPG A QK+E SEM +G AS +A MF E PGTS
Sbjct: 1091 SLVAFIFSGFKPNFSSTWSRIAGPPG*AIQKIEL*SEMFNGWKASINALSMFFETIPGTS 912
Query: 69 LSRWKVSPTSRRWPSMAPSESG 4
L R V P S R PSMAPS SG
Sbjct: 911 LRR*NVRPASTR*PSMAPSLSG 846
Score = 49.2 bits (101), Expect = 6e-007
Identities = 23/63 (36%), Positives = 38/63 (60%)
Frame = -2 / -2
Query: 421 AVEHDGLDGESRAEAEEDAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAVLGEDVAG 242
A+E++G +G+SR+EA++ PV+A + G S L L+HL+ DE+ HV + +
Sbjct: 1263 AMENNGFNGKSRSEAKQHTPVKAFSCGFKSRLCRFLSHLI*DEQYTCT*HVTIFTKYFPS 1084
Query: 241 GAH 233
G H
Sbjct: 1083 GLH 1075
>gb|L02902.1|ALFCHS2A Medicago sativa chalcone synthase (CHS2) mRNA, complete cds
Length = 1254
Score = 277 bits (600), Expect = 9e-076
Identities = 112/140 (80%), Positives = 126/140 (90%)
Frame = +2 / +2
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PLGISD+NSIFW+AHPGGP
Sbjct: 800 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNITKALVEAFEPLGISDYNSIFWIAHPGGP 979
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+ L +M ATR VLSEYGNMSSACVLFILDEMRK+S ++G TTGEGL+W
Sbjct: 980 AILDQVEQKLALKPEKMNATREVLSEYGNMSSACVLFILDEMRKKSTQNGLKTTGEGLEW 1159
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVL SV
Sbjct: 1160 GVLFGFGPGLTIETVVLRSV 1219
Score = 165 bits (355), Expect = 6e-042
Identities = 80/142 (56%), Positives = 93/142 (65%)
Frame = -3 / -1
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERT 250
+ TL TTVS VSPGPKP TP S PSPVV P + ISS++ +T ALD+ PYS T
Sbjct: 1224 IATLRKTTVSMVSPGPKPNNTPHSSPSPVVFNPF*VDFFLISSKINKTHALDIFPYSLST 1045
Query: 249 WRVARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTS 70
VA I + ST SR+AGPPGCA QK+E SE+P+G AS++A MF E PGTS
Sbjct: 1044 SLVAFIFSGFKANFCST*SRIAGPPGCAIQKIEL*SEIPNGSKASTNALVMFFETIPGTS 865
Query: 69 LSRWKVSPTSRRWPSMAPSESG 4
L R VSP SRR PSMAPS SG
Sbjct: 864 LRR*NVSPASRR*PSMAPSLSG 799
Score = 29.5 bits (58), Expect(2) = 1e-004
Identities = 13/53 (24%), Positives = 32/53 (60%)
Frame = -2 / -3
Query: 166 HPEDGVPVGDAERLERVVQRAADVLGDEPGHVLEQVEGEPHLAEVAVDGALRV 8
+P+D V + +++ LE + Q +DVL + P ++ ++V+ +V ++G+ +
Sbjct: 961 NPKD*VVIRNSQWLESLNQCFSDVL*NNPRNIFKKVKC*SSFTKVTINGSFTI 803
Score = 29.5 bits (58), Expect(2) = 1e-004
Identities = 12/53 (22%), Positives = 32/53 (60%)
Frame = -2 / -3
Query: 421 AVEHDGLDGESRAEAEEDAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAV 263
A +++ +G+S ++ ++ P ++ +R SVL L+H + D+++ H+++
Sbjct: 1216 ATQNNCFNGKSWSKTKQYTPFKSFSRCLQSVLS*LLSHFI*DKQNTCT*HISI 1058
>gb|L02904.1|ALFCHS8A Medicago sativa chalcone synthase (CHS8) mRNA, complete cds
Length = 1347
Score = 274 bits (592), Expect = 1e-074
Identities = 111/140 (79%), Positives = 126/140 (90%)
Frame = +2 / +1
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEG IDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PLGISD+NSIFW+AHPGGP
Sbjct: 784 PDSEGGIDGHLREAGLTFHLLKDVPGIVSKNINKALVEAFEPLGISDYNSIFWIAHPGGP 963
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+ L +M+ATR VLSEYGNMSSACVL ILDEMRK+SA+DG TTGEGL++
Sbjct: 964 AILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLVILDEMRKKSAQDGLKTTGEGLEF 1143
Query: 365 GVLFGFGPGLTVETVVLHSV 424
GVLFGFGPGLT+ETVVL SV
Sbjct: 1144 GVLFGFGPGLTIETVVLRSV 1203
Score = 166 bits (357), Expect = 3e-042
Identities = 80/142 (56%), Positives = 94/142 (66%)
Frame = -3 / -2
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERT 250
+ TL TTVS V PGPKP +TP S PSPVV PS A+ ISS++ +T ALD+ PYS T
Sbjct: 1208 IATLRKTTVSMVRPGPKPNKTPNSSPSPVVFSPS*ADFFLISSKITKTHALDIFPYSLST 1029
Query: 249 WRVARILALSSPTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTS 70
VA I + ST SR+AGPPGCA QK+E SE+P+G AS++A MF E PGTS
Sbjct: 1028 SLVAFIFSGFKANFCST*SRIAGPPGCAIQKIEL*SEIPNGSKASTNALLMFFETIPGTS 849
Query: 69 LSRWKVSPTSRRWPSMAPSESG 4
L R VSP SRR PSM PS SG
Sbjct: 848 LRR*NVSPASRR*PSMPPSLSG 783
Score = 44.6 bits (91), Expect(2) = 7e-008
Identities = 16/61 (26%), Positives = 39/61 (63%)
Frame = -2 / -1
Query: 415 EHDGLDGESRAEAEEDAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAVLGEDVAGGA 236
+++G +G++R++A+ + +++ +RG S+L L+H + D ++ H+++ E +GG
Sbjct: 1194 QNNGFNGKTRSKAK*NTKLKSFSRGLQSILS*LLSHFI*DNQNTCT*HISIFTEHFSGGF 1015
Query: 235 H 233
H
Sbjct: 1014 H 1012
Score = 25.8 bits (50), Expect(2) = 7e-008
Identities = 13/66 (19%), Positives = 35/66 (53%)
Frame = -2 / -1
Query: 205 LHLVEDGGPAGVRHPEDGVPVGDAERLERVVQRAADVLGDEPGHVLEQVEGEPHLAEVAV 26
L+L+ + V +P+D + + +++ + + Q DVL + PG++ ++V+ +V +
Sbjct: 984 LYLI*NCRSTRVCNPKD*IVIRNSQWFKSLNQCFIDVL*NNPGNIFKKVKC*SSFTKVTI 805
Query: 25 DGALRV 8
+ +
Sbjct: 804 NAPFTI 787
>gb|DQ122840.1| Medicago sativa clone K9 chalcone synthase mRNA, partial cds
Length = 660
Score = 265 bits (574), Expect = 4e-072
Identities = 106/131 (80%), Positives = 119/131 (90%)
Frame = +2 / +2
Query: 5 PDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGGP 184
PDSEGAIDGHLRE GLTFHLLKDVPG++SKNI +AL +AF+PL ISD+NSIFW+AHPGGP
Sbjct: 266 PDSEGAIDGHLREAGLTFHLLKDVPGIVSKNIDKALVEAFQPLNISDYNSIFWIAHPGGP 445
Query: 185 AILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTGEGLDW 364
AILDQVE K+GL +M+ATR VLSEYGNMSSACVLFILDEMRK+S +DG TTGEGL+W
Sbjct: 446 AILDQVEQKLGLKPEKMKATREVLSEYGNMSSACVLFILDEMRKKSTKDGLKTTGEGLEW 625
Query: 365 GVLFGFGPGLT 397
GVLFGFGPGLT
Sbjct: 626 GVLFGFGPGLT 658
Score = 150 bits (323), Expect = 1e-037
Identities = 74/131 (56%), Positives = 85/131 (64%)
Frame = -3 / -1
Query: 396 VSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQALDMLPYSERTWRVARILALSS 217
VSPGPKP TP S PSPVV PS + ISS++ +T ALD+ PYS T VA I +
Sbjct: 657 VSPGPKPNNTPHSSPSPVVFNPSLVDFFLISSKINKTHALDIFPYSLSTSLVAFIFSGFK 478
Query: 216 PTLASTWSRMAGPPGCATQKMEFQSEMPSGLNASSSARPMFLEMSPGTSLSRWKVSPTSR 37
P ST SR+AGPPG A QK+E SEM +G AS++A MF E PGTSL R V P SR
Sbjct: 477 PNFCST*SRIAGPPG*AIQKIEL*SEMFNGWKASTNALSMFFETIPGTSLRR*NVRPASR 298
Query: 36 RWPSMAPSESG 4
R PSMAPS SG
Sbjct: 297 R*PSMAPSLSG 265
>gb|CO514718.1|CO514718 s13dSG55C0800066_328096 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 388
Score = 232 bits (501), Expect = 4e-062
Identities = 93/116 (80%), Positives = 107/116 (92%)
Frame = +2 / +3
Query: 2 LPDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAHPGG 181
LPDS+GAIDGHLREVGLTFHLLKDVPG+ISKNI ++L +AF P+GISDWNSIFWVAHPGG
Sbjct: 39 LPDSDGAIDGHLREVGLTFHLLKDVPGIISKNIEKSLVEAFAPIGISDWNSIFWVAHPGG 218
Query: 182 PAILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKRSAEDGQATTG 349
PAILDQVE K+ L + ++R+TRHVLSEYGNMSSACVLFILDEMR+RS E+G+ TTG
Sbjct: 219 PAILDQVEEKLRLKEEKLRSTRHVLSEYGNMSSACVLFILDEMRRRSKEEGKITTG 386
Score = 148 bits (318), Expect = 7e-037
Identities = 68/117 (58%), Positives = 84/117 (71%)
Frame = -3 / -1
Query: 351 SPVVACPSSAERLRISSRMKRTQALDMLPYSERTWRVARILALSSPTLASTWSRMAGPPG 172
SPVV PSS + L ISS++ +TQALD+ PYS T RV R + + +ST S++AGPPG
Sbjct: 388 SPVVIFPSSLDLLLISSKINKTQALDIFPYSLSTCRVDRSFSSLRRSFSST*SKIAGPPG 209
Query: 171 CATQKMEFQSEMPSGLNASSSARPMFLEMSPGTSLSRWKVSPTSRRWPSMAPSESGR 1
CATQK+EFQS +P G AS+ +F E+ PGTS R KVSPTSRR PS+APSESGR
Sbjct: 208 CATQKIEFQSLIPIGAKASTKLFSIFFEIIPGTSFKR*KVSPTSRRCPSIAPSESGR 38
Score = 55.6 bits (115), Expect = 7e-009
Identities = 20/101 (19%), Positives = 56/101 (55%)
Frame = -2 / -3
Query: 310 HLVEDEEDAGAGHVAVLGEDVAGGAHPGLVQPDLGLHLVEDGGPAGVRHPEDGVPVGDAE 131
H ++++++ H+++ + V G L++ + +L+ + + +P+D +P+ +
Sbjct: 347 HFIQNKQNTSTRHISIFTKHVPSGPEFLLLKTEFFFNLI*NSRSTWMCNPKDRIPITYSN 168
Query: 130 RLERVVQRAADVLGDEPGHVLEQVEGEPHLAEVAVDGALRV 8
+ Q ++L D P ++ ++++ EPH E++V+ A+R+
Sbjct: 167 WCKSFNQTLLNIL*DNPRNIFQKMKSEPHFTEMSVNRAIRI 45
>gb|CO514656.1|CO514656 s13dSG46D1100094_327972 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 534
Score = 124 bits (265), Expect = 1e-029
Identities = 48/57 (84%), Positives = 54/57 (94%)
Frame = +2 / +1
Query: 2 LPDSEGAIDGHLREVGLTFHLLKDVPGLISKNIGRALDDAFKPLGISDWNSIFWVAH 172
LPDS+GAIDGHLREVGLTFHLLKDVPG+ISKNI ++L +AF P+GISDWNSIFWVAH
Sbjct: 364 LPDSDGAIDGHLREVGLTFHLLKDVPGIISKNIEKSLVEAFAPIGISDWNSIFWVAH 534
Score = 84.5 bits (178), Expect = 1e-017
Identities = 37/57 (64%), Positives = 43/57 (75%)
Frame = -3 / -2
Query: 171 CATQKMEFQSEMPSGLNASSSARPMFLEMSPGTSLSRWKVSPTSRRWPSMAPSESGR 1
CATQK+EFQS +P G AS+ +F E+ PGTS R KVSPTSRR PS+APSESGR
Sbjct: 533 CATQKIEFQSLIPIGAKASTKLFSIFFEIIPGTSFRR*KVSPTSRRCPSIAPSESGR 363
Score = 37.3 bits (75), Expect = 0.002
Identities = 13/53 (24%), Positives = 33/53 (62%)
Frame = -2 / -1
Query: 166 HPEDGVPVGDAERLERVVQRAADVLGDEPGHVLEQVEGEPHLAEVAVDGALRV 8
+P+D +P+ + + Q ++L D P ++ ++++ EP+ E++V+ A+R+
Sbjct: 528 NPKD*IPITYSNWCKSFNQTLLNIL*DNPRNIFQKMKSEPNFTEMSVNRAIRI 370
>gb|CO514834.1|CO514834 s13dSG59G1200100_328328 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 568
Score = 39.1 bits (79), Expect = 7e-004
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +2 / +2
Query: 170 HPGGPAILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFIL 301
H GG A+LD++E + L M +R L+ +GN SS+ + + L
Sbjct: 140 HAGGRAVLDELEKNLDLSDWHMEPSRMTLNRFGNTSSSSLWYEL 271
>gb|CO516112.1|CO516112 s13dSG65D1200102_445552 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 565
Score = 35.9 bits (72), Expect = 0.006
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +2 / +3
Query: 170 HPGGPAILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKR 319
HPGG A++D V + L++ + R L +GN S+ + ++L M +
Sbjct: 147 HPGGRAVIDGVGKGLTLNEYDLEPARMALHRWGNTSAGGLWYVLGYMEAK 296
>gb|CO516656.1|CO516656 s13dSG66G0900070_446640 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 567
Score = 35.9 bits (72), Expect = 0.006
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +2 / +1
Query: 170 HPGGPAILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKR 319
HPGG A++D V + L++ + R L +GN S+ + ++L M +
Sbjct: 43 HPGGRAVIDGVGKGLTLNEYDLEPARMALHRWGNTSAGGLWYVLGYMEAK 192
>gb|CO517196.1|CO517196 s13dSG33G1100088_474476 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 649
Score = 35.9 bits (72), Expect = 0.006
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +2 / -3
Query: 170 HPGGPAILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKR 319
HPGG A++D V + L++ + R L +GN S+ + ++L M +
Sbjct: 572 HPGGRAVIDGVGKGLTLNEYDLEPARMALHRWGNTSAGGLWYVLGYMEAK 423
>gb|CO515474.1|CO515474 s13dSG52A0100005_418007 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 427
Score = 35.0 bits (70), Expect = 0.012
Identities = 15/50 (30%), Positives = 25/50 (50%)
Frame = +2 / +1
Query: 170 HPGGPAILDQVEAKVGLDKARMRATRHVLSEYGNMSSACVLFILDEMRKR 319
H ILD+++ + L M A+R L +GN SS+ + + L M +
Sbjct: 19 HAASKTILDELQKNLELSDKNMEASRMTLHRFGNTSSSSIWYELAYMEAK 168
>gb|U89923.1|MSU89923 Medicago sativa asparagine synthetase mRNA, complete cds
Length = 2184
Score = 31.8 bits (63), Expect = 0.11
Identities = 14/37 (37%), Positives = 19/37 (51%)
Frame = +1 / +3
Query: 619 YLYICISCKTRVELA*GEDPYRCHFSMHGVVRYWVYL 729
YL+IC +R + E YRCHF +H + W L
Sbjct: 540 YLFICSVGYSRQQFHCCEGCYRCHFLVHWLGTRWFCL 650
>gb|L40327.1|ALFASPSYN Medicago sativa asparagine synthetase gene, exons 1-13 and complete
cds
Length = 7969
Score = 31.3 bits (62), Expect = 0.15
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +1 / +2
Query: 619 YLYICISCKTRVELA*GEDPYRCHFSMHGVVRYW 720
YL+IC +R + E YRCHF +H + W
Sbjct: 4346 YLFICSVGYSRQQFHCCEGCYRCHFLVHWLGTRW 4447
>gb|DR025668.2|DR025668 EST12 Medicago sativa cDNA-AFLP (Zou and Gong) Medicago sativa cDNA
clone M18 5', mRNA sequence
Length = 392
Score = 28.6 bits (56), Expect = 0.99
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -2 / +2
Query: 232 PGLVQPDLGLHLVEDGGPAGVRHPEDG 152
P LV+P L H G VRHP+ G
Sbjct: 269 PVLVEPSLKYHPYRHGCLTAVRHPDPG 349
>gb|AY639647.1| Medicago sativa GMPase (GMP) mRNA, complete cds
Length = 1181
Score = 28.6 bits (56), Expect = 0.99
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -3 / -2
Query: 411 TTVSTVSPGPKPKRTPQSRPSPVVACPSS 325
T ST+ PGP P PSP+ A S+
Sbjct: 949 TPDSTMQPGPIATSGPMRHPSPIFAVSST 863
>gb|CO516793.1|CO516793 s13dSG94E0900069_446914 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 125
Score = 27.6 bits (54), Expect = 1.9
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +2 / -1
Query: 578 FPCTVSRTIIIYNNISI 628
+PC++S II YNNI I
Sbjct: 119 WPCSISIVIIYYNNIII 69
>gb|CO511922.1|CO511922 s13dSG05F0200027_103744 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 604
Score = 26.7 bits (52), Expect = 3.5
Identities = 17/51 (33%), Positives = 22/51 (43%)
Frame = -2 / -1
Query: 370 DAPVEALARGGLSVLGGALAHLVEDEEDAGAGHVAVLGEDVAGGAHPGLVQ 218
D VEA GG GGA ++ + + G V G V GG G V+
Sbjct: 388 DGGVEAGVVGGGVEGGGAEGAIIGGDGEGGVEGGGVEGSTVGGGVEGGGVE 236
>gb|CO515174.1|CO515174 s13dSG62C0600050_399213 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 26.7 bits (52), Expect = 3.5
Identities = 10/24 (41%), Positives = 14/24 (58%)
Frame = -3 / +1
Query: 393 SPGPKPKRTPQSRPSPVVACPSSA 322
SP P PK+ P + P + PSS+
Sbjct: 355 SPPPSPKKPPSANCPPPPSSPSSS 426
>gb|CO516473.1|CO516473 s13dSG28C1200098_446274 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 560
Score = 26.7 bits (52), Expect = 3.5
Identities = 10/32 (31%), Positives = 16/32 (50%)
Frame = -3 / +1
Query: 420 LWSTTVSTVSPGPKPKRTPQSRPSPVVACPSS 325
L +T+ +PG P + P + P+P P S
Sbjct: 109 LLATSCFAQAPGAAPTQPPSATPTPPTPAPKS 204
>gb|CO516916.1|CO516916 s13dSG58D0800064_468378 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 517
Score = 26.7 bits (52), Expect = 3.5
Identities = 14/32 (43%), Positives = 16/32 (50%)
Frame = -2 / -2
Query: 409 DGLDGESRAEAEEDAPVEALARGGLSVLGGAL 314
D LDG SRAE EA A S+ GG +
Sbjct: 306 DSLDGASRAELSCPEATEAEASPSSSLEGGVI 211
>gb|CO512060.1|CO512060 s13dSG18D0800062_108440 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 597
Score = 26.3 bits (51), Expect = 4.8
Identities = 8/13 (61%), Positives = 12/13 (92%)
Frame = -2 / +1
Query: 589 STWKNSISYLLLH 551
STWKNS++ +L+H
Sbjct: 379 STWKNSVTTVLVH 417
>gb|CO516827.1|CO516827 s13dSG98C0200006_446982 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 417
Score = 26.3 bits (51), Expect = 4.8
Identities = 14/40 (35%), Positives = 21/40 (52%)
Frame = -2 / +2
Query: 586 TWKNSISYLLLHTTIIAESTLLFADGRRKIMTNETTTNWI 467
T KNS+S L H +A +DG I +N+T ++I
Sbjct: 248 TLKNSLSIALSHFPPLAGRLTTDSDGYIHITSNDTGVDFI 367
>gb|CO516836.1|CO516836 s13dSG98D0200014_447000 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 391
Score = 26.3 bits (51), Expect = 4.8
Identities = 13/50 (26%), Positives = 21/50 (42%)
Frame = -3 / +1
Query: 429 MGTLWSTTVSTVSPGPKPKRTPQSRPSPVVACPSSAERLRISSRMKRTQA 280
M L + + +P P P P P+P P AE +++ K+ A
Sbjct: 91 MAELQTKQEAVPAPAPAPAPAPVPTPAPAAVEPPLAEAPPVNAVEKKAAA 240
>gb|L01660.1|ALFNADHSYN Medicago sativa NADH-glutamate synthase mRNA, comlete cds
Length = 7174
Score = 25.8 bits (50), Expect(2) = 5.5
Identities = 7/19 (36%), Positives = 14/19 (73%)
Frame = -3 / -1
Query: 267 PYSERTWRVARILALSSPT 211
P+SER W + ++++ SP+
Sbjct: 2242 PFSERRWHCSSVVSVRSPS 2186
Score = 22.1 bits (42), Expect(2) = 5.5
Identities = 8/23 (34%), Positives = 12/23 (52%)
Frame = -3 / -1
Query: 378 PKRTPQSRPSPVVACPSSAERLR 310
P+R + PV +CPS +R
Sbjct: 3916 PRRAAMATSLPVFSCPSVCRVVR 3848
>gb|L37606.1|ALFNDGS Medicago sativa (clone GG16-1) NADH-dependent glutamate synthase
gene, complete cds
Length = 14306
Score = 25.8 bits (50), Expect = 6.6
Identities = 7/19 (36%), Positives = 14/19 (73%)
Frame = -3 / -1
Query: 267 PYSERTWRVARILALSSPT 211
P+SER W + ++++ SP+
Sbjct: 7493 PFSERRWHCSSVVSVRSPS 7437
>gb|CO513867.1|CO513867 s13dSG73E0700051_156396 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 222
Score = 25.4 bits (49), Expect = 9.1
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = -3 / -2
Query: 408 TVSTVSPGPKPKRTPQSRPSP 346
T+S +P PKP P +P P
Sbjct: 176 TLSPPAPPPKPPPAPPPKPPP 114
>gb|L36120.1|ALFMSA3S Medicago sativa proline-rich protein mRNA, complete cds
Length = 1018
Score = 25.4 bits (49), Expect = 9.1
Identities = 10/28 (35%), Positives = 13/28 (46%)
Frame = -3 / +2
Query: 414 STTVSTVSPGPKPKRTPQSRPSPVVACP 331
S+ P P TPQ+ P PV + P
Sbjct: 194 SSPAPPTPPANTPPTTPQASPPPVQSSP 277
>gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRNA, complete cds
Length = 2760
Score = 25.4 bits (49), Expect = 9.1
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = -3 / -1
Query: 42 SRRWPSMAPSESGR 1
S RWP + P+ SGR
Sbjct: 1116 SSRWPQVVPTASGR 1075
>gb|AF417491.1| Medicago sativa RING-H2 protein (RH2-1) gene, complete cds
Length = 3970
Score = 25.4 bits (49), Expect = 9.1
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = +1 / +1
Query: 598 HNHHI**YLYICISC 642
H+HHI Y YI I+C
Sbjct: 1780 HHHHIISYTYIKIAC 1824
>emb|X88845.1|MSNUM1GEN M.sativa mRNA for nuM1 protein
Length = 2255
Score = 25.4 bits (49), Expect = 9.1
Identities = 9/25 (36%), Positives = 12/25 (48%)
Frame = -3 / -1
Query: 438 PVVMGTLWSTTVSTVSPGPKPKRTP 364
P + LW+ + S PKP TP
Sbjct: 1406 PFISSALWAASAVANSTCPKPLNTP 1332
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,063,388
Number of Sequences: 7669
Number of extensions: 56755
Number of successful extensions: 799
Number of sequences better than 10.0: 38
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 195485170
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)