TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716450.2.1
(2214 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514334.1|CO514334 s13dSG84C0600038_157330 Glandular tr... 232 1e-061
gb|AY912107.1| Medicago satvia SSR marker Agat004 sequence 78 7e-029
>gb|CO514334.1|CO514334 s13dSG84C0600038_157330 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 492
Score = 232 bits (501), Expect = 1e-061
Identities = 95/115 (82%), Positives = 106/115 (92%)
Frame = -2 / +2
Query: 1817 RFSTAAAVEEPITPSVHVNYTKLLINGNFVDSASGKTFPTLDPRTGEVIAHVAEGDAEDI 1638
+FSTAAAVEE ITP V +NYT+ LING FVD+ASGKTFPTLDPRTGEVIA+VAEGDAEDI
Sbjct: 146 KFSTAAAVEELITPQVSINYTQHLINGKFVDAASGKTFPTLDPRTGEVIANVAEGDAEDI 325
Query: 1637 NRAVAAARKAFDEGPWPKMTAYERSRILLRFADLIEKHNDELAALETWDNGKPYE 1473
NRAV+AAR+AFD GPWPKM+AYER RILLRFADL+EKHNDE+AALE W+NGK YE
Sbjct: 326 NRAVSAAREAFDNGPWPKMSAYERCRILLRFADLVEKHNDEIAALEAWNNGKLYE 490
Score = 144 bits (310), Expect = 3e-035
Identities = 71/116 (61%), Positives = 76/116 (65%)
Frame = +1 / -1
Query: 1471 CSYGFPLSHVSKAASSSLCFSIKSANRSRIRDLS*AVIFGHGPSSKALRAAATARLMXXX 1650
CS FPL H S+AA SSLCFS KSA RS IR LS*A+I G GP SKA RAA TARL+
Sbjct: 492 CS*SFPLFHASRAAISSLCFSTKSAKRSNIRHLS*ALILGQGPLSKASRAADTARLISSA 313
Query: 1651 XXXXXXXXXXXVRGSRVGKVLPDAESTKFPLMRSFV*FTWTDGVMGSSTAAAVLNL 1818
VRGS VG V PDA ST FP +R V*F T GV+ SSTAAAVLNL
Sbjct: 312 SPSATLAITSPVRGSSVGNVFPDAASTNFPFIRCCV*FIETCGVINSSTAAAVLNL 145
Score = 121 bits (258), Expect = 4e-028
Identities = 53/113 (46%), Positives = 72/113 (63%)
Frame = +3 / -3
Query: 1476 IWLPVVPCLQSSKLVIVLLYQISKPQ*DTGPLIGSHLRPWPFIKSLASRSYCTVNVLCVT 1655
I L +VPCLQS L+IVLL QISK Q T P IG+H RP IK LAS YCTVN+ +T
Sbjct: 487 IKLSIVPCLQSCNLIIVLLNQISKTQQYTTPFIGTHFRPRSIIKGLASC*YCTVNIFSIT 308
Query: 1656 LSHMSNHLPCTRVQSWEGLAGCGINKVPINEELCVVHMD*RRDGLLYCCCSAE 1814
S++SNH PCT ++ W+ C I+K+ I + LC+++ + + L C A+
Sbjct: 307 FSNISNHFPCTWIKCWKCFP*CSIHKLSIYKMLCIIY*NLWSNQFLDSCSGAK 149
>gb|AY912107.1| Medicago satvia SSR marker Agat004 sequence
Length = 594
Score = 78.0 bits (164), Expect(2) = 7e-029
Identities = 33/38 (86%), Positives = 34/38 (89%)
Frame = -2 / -3
Query: 425 FGGYKMSGIGREKGVDSLKNYLQVKAVVTPIKNAAWL* 312
FGGYKMSG GREKG SLKNYLQVKAVVTP+KN AWL*
Sbjct: 505 FGGYKMSGQGREKGEYSLKNYLQVKAVVTPLKNPAWL* 392
Score = 65.2 bits (136), Expect(2) = 7e-029
Identities = 24/30 (80%), Positives = 27/30 (90%)
Frame = -2 / -1
Query: 515 SLDTANTLTRALRAGTVWVNCFDVFDAAIP 426
++DTANTLTRAL+ GTVWVNCFD FDAA P
Sbjct: 594 NIDTANTLTRALKVGTVWVNCFDTFDAAXP 505
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 18,692,215
Number of Sequences: 7669
Number of extensions: 262874
Number of successful extensions: 1824
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 45
effective length of database: 903,463
effective search space used: 625196396
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)