TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716424.2.5
(1999 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 78 1e-057
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 78 8e-057
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 104 1e-051
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 64 3e-045
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 64 9e-045
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 64 1e-044
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 72 1e-035
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 100 3e-030
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 78.0 bits (164), Expect(5) = 1e-057
Identities = 33/86 (38%), Positives = 53/86 (61%)
Frame = -2 / +2
Query: 1599 GLLLLRIGAVPTLFVSSPSAADAVLRTQDHIFASRPPSMAAEIIRYGPSDVAFVPYGEYW 1420
G+ LR+G + + +S AA VL+ QD+IF++RP ++A + + Y +D+AF YG +W
Sbjct: 305 GIFHLRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYLTYDRADMAFAHYGPFW 484
Query: 1419 RQGRKLLTTHMLSTKKVQSFRHGRQE 1342
RQ RKL + S K +S++ R E
Sbjct: 485 RQMRKLCVMKLFSRKHAESWQSVRDE 562
Score = 73.0 bits (153), Expect(5) = 1e-057
Identities = 24/50 (48%), Positives = 36/50 (72%)
Frame = -2 / +2
Query: 666 LLPHYSTADSEIDGYFVPAGTRVLVNAWALGRDRTTWEKPEEFMPERFVQ 517
LL H + ++ ++GYF+P RV++NAWA+GRD WE+PE F P RF++
Sbjct: 1226 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRFLK 1375
Score = 67.5 bits (141), Expect(5) = 1e-057
Identities = 25/54 (46%), Positives = 34/54 (62%)
Frame = -2 / +2
Query: 489 KGKDLRFIPFGSGRRICPGMNFGFATMEIMLANLMYHFDWEVPGSAAGVSMDES 328
KG + FIPFGSGRR CPGM G +++ +A+L++ F WE+P MD S
Sbjct: 1394 KGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMS 1555
Score = 46.0 bits (94), Expect(5) = 1e-057
Identities = 19/46 (41%), Positives = 31/46 (67%)
Frame = -2 / +2
Query: 930 LTTDNLKAILVDMFEAGIETSYLTLEYGMAELINNRHVMEKLQTEV 793
LT DN+KAI++D+ G ET +E+ MAEL+ + ++K+Q E+
Sbjct: 980 LTKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQEL 1117
Score = 44.6 bits (91), Expect(5) = 1e-057
Identities = 17/28 (60%), Positives = 19/28 (67%)
Frame = -2 / +2
Query: 744 EEDLGSMPYLKATIKETLRLHPPAPFLL 661
E D + YLK +KETLRLHPP P LL
Sbjct: 1151 EPDFEKLTYLKCALKETLRLHPPIPLLL 1234
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 78.0 bits (164), Expect(5) = 8e-057
Identities = 33/86 (38%), Positives = 53/86 (61%)
Frame = -2 / +1
Query: 1599 GLLLLRIGAVPTLFVSSPSAADAVLRTQDHIFASRPPSMAAEIIRYGPSDVAFVPYGEYW 1420
G+ LR+G + + +S AA VL+ QD+IF++RP ++A + + Y +D+AF YG +W
Sbjct: 319 GIFHLRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYLTYDRADMAFAHYGPFW 498
Query: 1419 RQGRKLLTTHMLSTKKVQSFRHGRQE 1342
RQ RKL + S K +S++ R E
Sbjct: 499 RQMRKLCVMKLFSRKHAESWQSVRDE 576
Score = 71.6 bits (150), Expect(5) = 8e-057
Identities = 23/50 (46%), Positives = 36/50 (72%)
Frame = -2 / +1
Query: 666 LLPHYSTADSEIDGYFVPAGTRVLVNAWALGRDRTTWEKPEEFMPERFVQ 517
LL H + ++ ++GYF+P RV++NAWA+GRD W++PE F P RF++
Sbjct: 1240 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLK 1389
Score = 67.5 bits (141), Expect(5) = 8e-057
Identities = 25/54 (46%), Positives = 34/54 (62%)
Frame = -2 / +1
Query: 489 KGKDLRFIPFGSGRRICPGMNFGFATMEIMLANLMYHFDWEVPGSAAGVSMDES 328
KG + FIPFGSGRR CPGM G +++ +A+L++ F WE+P MD S
Sbjct: 1408 KGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMS 1569
Score = 44.6 bits (91), Expect(5) = 8e-057
Identities = 17/28 (60%), Positives = 19/28 (67%)
Frame = -2 / +1
Query: 744 EEDLGSMPYLKATIKETLRLHPPAPFLL 661
E D + YLK +KETLRLHPP P LL
Sbjct: 1165 EPDFEKLTYLKCALKETLRLHPPIPLLL 1248
Score = 44.6 bits (91), Expect(5) = 8e-057
Identities = 19/46 (41%), Positives = 31/46 (67%)
Frame = -2 / +1
Query: 930 LTTDNLKAILVDMFEAGIETSYLTLEYGMAELINNRHVMEKLQTEV 793
LT DN+KAI++D+ G ET +E+ MAEL+ + ++K+Q E+
Sbjct: 994 LTKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQEL 1131
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase mRNA,
complete cds
Length = 1744
Score = 104 bits (222), Expect(4) = 1e-051
Identities = 40/86 (46%), Positives = 59/86 (68%)
Frame = -2 / +3
Query: 750 LAEEDLGSMPYLKATIKETLRLHPPAPFLLPHYSTADSEIDGYFVPAGTRVLVNAWALGR 571
+ E DL +PYL+A IKETLRL P L+PH + D +++G+ +PA +++LVNAW L
Sbjct: 1068 VTEPDLHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDPKLNGFDIPAESKILVNAWWLPN 1247
Query: 570 DRTTWEKPEEFMPERFVQEPGAVDVH 493
+ W+KPEEF PERF++E V+ +
Sbjct: 1248 NPAHWKKPEEFRPERFLEEESHVEAN 1325
Score = 73.5 bits (154), Expect(4) = 1e-051
Identities = 33/95 (34%), Positives = 53/95 (55%)
Frame = -2 / +3
Query: 1596 LLLLRIGAVPTLFVSSPSAADAVLRTQDHIFASRPPSMAAEIIRYGPSDVAFVPYGEYWR 1417
+ LLR+G + VSSP A VL TQ F SR ++ +I D+ F YGE+WR
Sbjct: 231 IFLLRMGQRNLVVVSSPELAKEVLHTQCVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWR 410
Query: 1416 QGRKLLTTHMLSTKKVQSFRHGRQEEVRLVMDKIR 1312
+ R+++T + K VQ +R+G + E V++ ++
Sbjct: 411 KMRRIMTVPFFTNKVVQQYRYGWESEAESVVNDVK 515
Score = 45.1 bits (92), Expect(4) = 1e-051
Identities = 21/77 (27%), Positives = 46/77 (59%)
Frame = -2 / +3
Query: 1023 QEHVQSRPSGESEESEADFIHVLLSIQQEYGLTTDNLKAILVDMFEAGIETSYLTLEYGM 844
++ ++S S S + I +L Q++ + DN+ I+ ++ A IET+ ++E+G+
Sbjct: 810 RKKLESTKSTTSNDGLKCAIDHILDAQKKGEINDDNVLYIVENINVAAIETTLWSIEWGI 989
Query: 843 AELINNRHVMEKLQTEV 793
AEL+N++ + K++ E+
Sbjct: 990 AELVNHQDIQNKVREEM 1040
Score = 44.6 bits (91), Expect(4) = 1e-051
Identities = 18/59 (30%), Positives = 28/59 (47%)
Frame = -2 / +3
Query: 498 VHMKGKDLRFIPFGSGRRICPGMNFGFATMEIMLANLMYHFDWEVPGSAAGVSMDESFG 322
V G D R++PFG GRR CPG+ + I + L+ + + P + + E G
Sbjct: 1314 VEANGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNVELLPPPGQSKIDTSEKGG 1490
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 63.8 bits (133), Expect(6) = 3e-045
Identities = 21/44 (47%), Positives = 30/44 (68%)
Frame = -2 / +3
Query: 648 TADSEIDGYFVPAGTRVLVNAWALGRDRTTWEKPEEFMPERFVQ 517
T + EI+GY +P G +L N W +GRD W++P EF PERF++
Sbjct: 1089 TEECEINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFLE 1220
Score = 55.1 bits (114), Expect(6) = 3e-045
Identities = 22/53 (41%), Positives = 35/53 (66%)
Frame = -2 / +3
Query: 516 EPGAVDVHMKGKDLRFIPFGSGRRICPGMNFGFATMEIMLANLMYHFDWEVPG 358
E A + ++G+ + +PFGSGRR+CPG+N + M +LA+L+ FD +V G
Sbjct: 1230 EGEARPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQVLG 1388
Score = 53.8 bits (111), Expect(6) = 3e-045
Identities = 21/49 (42%), Positives = 34/49 (69%)
Frame = -2 / +3
Query: 939 EYGLTTDNLKAILVDMFEAGIETSYLTLEYGMAELINNRHVMEKLQTEV 793
E +T D++K ++VD F AG +++ + E+ +AELINN V+EK + EV
Sbjct: 816 EIKITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEV 962
Score = 41.4 bits (84), Expect(6) = 3e-045
Identities = 13/28 (46%), Positives = 21/28 (75%)
Frame = -2 / +3
Query: 753 MLAEEDLGSMPYLKATIKETLRLHPPAP 670
++ E D ++PY++A +KET R+HPP P
Sbjct: 987 LVDEVDTQNLPYIRAIVKETFRMHPPLP 1070
Score = 41.4 bits (84), Expect(6) = 3e-045
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = -2 / +3
Query: 1455 SDVAFVPYGEYWRQGRKLLTTHMLSTKKVQSFRHGRQEEVRLVM 1324
S VA VP+G YW+ RKL+ +L+ V R R +++R ++
Sbjct: 309 SSVAMVPFGPYWKFVRKLIMNDLLNATTVNKLRPLRTQQIRKLL 440
Score = 28.6 bits (56), Expect(6) = 3e-045
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -2 / +3
Query: 360 GSAAGVSMDESFGLTLRRKEKLLLVP 283
G A VSM+E GLT+ R L+ VP
Sbjct: 1410 GGDAKVSMEERAGLTVPRAHSLVCVP 1487
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 64.3 bits (134), Expect(6) = 9e-045
Identities = 21/44 (47%), Positives = 30/44 (68%)
Frame = -2 / +3
Query: 648 TADSEIDGYFVPAGTRVLVNAWALGRDRTTWEKPEEFMPERFVQ 517
T + EI+GY +P G +L N W +GRD W++P EF PERF++
Sbjct: 1089 TEECEINGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERFLE 1220
Score = 55.1 bits (114), Expect(6) = 9e-045
Identities = 22/53 (41%), Positives = 35/53 (66%)
Frame = -2 / +3
Query: 516 EPGAVDVHMKGKDLRFIPFGSGRRICPGMNFGFATMEIMLANLMYHFDWEVPG 358
E A + ++G+ + +PFGSGRR+CPG+N + M +LA+L+ FD +V G
Sbjct: 1230 EGEARPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQVLG 1388
Score = 53.8 bits (111), Expect(6) = 9e-045
Identities = 21/49 (42%), Positives = 34/49 (69%)
Frame = -2 / +3
Query: 939 EYGLTTDNLKAILVDMFEAGIETSYLTLEYGMAELINNRHVMEKLQTEV 793
E +T D++K ++VD F AG +++ + E+ +AELINN V+EK + EV
Sbjct: 816 EIKITKDHIKGLVVDFFSAGTDSTAVATEWALAELINNPKVLEKAREEV 962
Score = 41.4 bits (84), Expect(6) = 9e-045
Identities = 13/28 (46%), Positives = 21/28 (75%)
Frame = -2 / +3
Query: 753 MLAEEDLGSMPYLKATIKETLRLHPPAP 670
++ E D ++PY++A +KET R+HPP P
Sbjct: 987 LVDEVDTQNLPYIRAIVKETFRMHPPLP 1070
Score = 39.1 bits (79), Expect(6) = 9e-045
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = -2 / +3
Query: 1455 SDVAFVPYGEYWRQGRKLLTTHMLSTKKVQSFRHGRQEEVR 1333
S VA P+G YW+ RKL+ +L+ V R R +++R
Sbjct: 309 SSVAMAPFGPYWKFVRKLIMNDLLNATTVNKLRPLRTQQIR 431
Score = 28.6 bits (56), Expect(6) = 9e-045
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -2 / +3
Query: 360 GSAAGVSMDESFGLTLRRKEKLLLVP 283
G A VSM+E GLT+ R L+ VP
Sbjct: 1410 GGDAKVSMEERAGLTVPRAHSLVCVP 1487
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 64.3 bits (134), Expect(6) = 1e-044
Identities = 22/44 (50%), Positives = 30/44 (68%)
Frame = -2 / +3
Query: 648 TADSEIDGYFVPAGTRVLVNAWALGRDRTTWEKPEEFMPERFVQ 517
T + EI+GY +P G VL N W +GRD W++P EF PERF++
Sbjct: 1089 TEECEINGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERFLE 1220
Score = 55.1 bits (114), Expect(6) = 1e-044
Identities = 22/53 (41%), Positives = 35/53 (66%)
Frame = -2 / +3
Query: 516 EPGAVDVHMKGKDLRFIPFGSGRRICPGMNFGFATMEIMLANLMYHFDWEVPG 358
E A + ++G+ + +PFGSGRR+CPG+N + M +LA+L+ FD +V G
Sbjct: 1230 EGEAGPLDLRGQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQVLG 1388
Score = 51.5 bits (106), Expect(6) = 1e-044
Identities = 19/49 (38%), Positives = 34/49 (69%)
Frame = -2 / +3
Query: 939 EYGLTTDNLKAILVDMFEAGIETSYLTLEYGMAELINNRHVMEKLQTEV 793
E +T + +K ++VD+F AG +++ + E+ +AELINN V++K + EV
Sbjct: 816 EIKITKEQIKGLVVDLFSAGTDSTAVATEWALAELINNPRVLQKAREEV 962
Score = 41.4 bits (84), Expect(6) = 1e-044
Identities = 13/28 (46%), Positives = 21/28 (75%)
Frame = -2 / +3
Query: 753 MLAEEDLGSMPYLKATIKETLRLHPPAP 670
++ E D ++PY++A +KET R+HPP P
Sbjct: 987 LVDEVDTQNLPYIRAIVKETFRMHPPLP 1070
Score = 40.9 bits (83), Expect(6) = 1e-044
Identities = 16/41 (39%), Positives = 24/41 (58%)
Frame = -2 / +3
Query: 1455 SDVAFVPYGEYWRQGRKLLTTHMLSTKKVQSFRHGRQEEVR 1333
+ VA VP+G YWR RKL+ +L+ V R R +++R
Sbjct: 309 NSVAMVPFGPYWRFVRKLIMNDLLNATTVNKLRPLRTQQIR 431
Score = 28.6 bits (56), Expect(6) = 1e-044
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = -2 / +3
Query: 360 GSAAGVSMDESFGLTLRRKEKLLLVP 283
G A VSM+E GLT+ R L+ VP
Sbjct: 1410 GDDAKVSMEERAGLTVPRAHSLVCVP 1487
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 71.6 bits (150), Expect(3) = 1e-035
Identities = 23/50 (46%), Positives = 36/50 (72%)
Frame = -2 / +2
Query: 666 LLPHYSTADSEIDGYFVPAGTRVLVNAWALGRDRTTWEKPEEFMPERFVQ 517
LL H + ++ ++GYF+P RV++NAWA+GRD W++PE F P RF++
Sbjct: 86 LLLHETAEEATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLK 235
Score = 67.5 bits (141), Expect(3) = 1e-035
Identities = 25/54 (46%), Positives = 34/54 (62%)
Frame = -2 / +2
Query: 489 KGKDLRFIPFGSGRRICPGMNFGFATMEIMLANLMYHFDWEVPGSAAGVSMDES 328
KG + FIPFGSGRR CPGM G +++ +A+L++ F WE+P MD S
Sbjct: 254 KGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPDGMKPSEMDMS 415
Score = 44.6 bits (91), Expect(3) = 1e-035
Identities = 17/28 (60%), Positives = 19/28 (67%)
Frame = -2 / +2
Query: 744 EEDLGSMPYLKATIKETLRLHPPAPFLL 661
E D + YLK +KETLRLHPP P LL
Sbjct: 11 EPDFEKLTYLKCALKETLRLHPPIPLLL 94
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 100 bits (214), Expect(3) = 3e-030
Identities = 40/75 (53%), Positives = 51/75 (68%)
Frame = -2 / +2
Query: 744 EEDLGSMPYLKATIKETLRLHPPAPFLLPHYSTADSEIDGYFVPAGTRVLVNAWALGRDR 565
E +L + YLK+ IKET+RLHP P L+P S +I+GY +PA TRV+VNAWA+GRD
Sbjct: 227 ETELHQLIYLKSVIKETMRLHPSLPLLIPRESIKPCQINGYDIPAKTRVIVNAWAIGRDP 406
Query: 564 TTWEKPEEFMPERFV 520
W E F PERF+
Sbjct: 407 RYWVDAESFKPERFL 451
Score = 40.5 bits (82), Expect(3) = 3e-030
Identities = 18/38 (47%), Positives = 24/38 (63%)
Frame = -2 / +2
Query: 903 LVDMFEAGIETSYLTLEYGMAELINNRHVMEKLQTEVR 790
L DMF G E+S + + M+E++ N VMEK Q EVR
Sbjct: 83 LQDMFVGGTESSSEVVLWAMSEMVKNPMVMEKAQVEVR 196
Score = 24.0 bits (46), Expect(3) = 3e-030
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = -2 / +2
Query: 489 KGKDLRFIPFGS 454
KG D +IPFGS
Sbjct: 470 KGTDFEYIPFGS 505
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,349,450
Number of Sequences: 7669
Number of extensions: 170011
Number of successful extensions: 1221
Number of sequences better than 1.0e-020: 8
length of database: 1,248,568
effective HSP length: 45
effective length of database: 903,463
effective search space used: 560147060
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)