TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716424.2.3
(1842 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 71 6e-044
gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-... 71 1e-043
gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible c... 84 1e-043
gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase ... 70 1e-028
gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase ... 70 2e-028
gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase ... 66 9e-028
gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular tr... 66 5e-024
gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular tr... 92 1e-021
gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular tr... 64 8e-021
>gb|DQ222912.1| Medicago sativa ferulate 5-hydroxylase (F5H-K10) mRNA, complete cds
Length = 1885
Score = 70.7 bits (148), Expect(4) = 6e-044
Identities = 31/88 (35%), Positives = 50/88 (56%)
Frame = +2 / +2
Query: 281 HNGFLFLRAGAVPTLIVSSPSAAEAVMRTHDHIFASRPWSMASHILRYNTCDVAFSPLGE 460
+ G LR G + + +S AA V++ D+IF++RP ++A L Y+ D+AF+ G
Sbjct: 299 YGGIFHLRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYLTYDRADMAFAHYGP 478
Query: 461 YWQQTRKLMNTHLLSNKKVYSFRHGREE 544
+W+Q RKL L S K S++ R+E
Sbjct: 479 FWRQMRKLCVMKLFSRKHAESWQSVRDE 562
Score = 67.0 bits (140), Expect(4) = 6e-044
Identities = 22/44 (50%), Positives = 30/44 (68%)
Frame = +2 / +2
Query: 1235 DCQIDGYHIPANTRVLVNGWAIGRDPAVWEKPEEFMPERFMRDG 1366
+ ++GY IP RV++N WAIGRD WE+PE F P RF++ G
Sbjct: 1250 EATVNGYFIPKQARVMINAWAIGRDANCWEEPESFKPSRFLKPG 1381
Score = 61.1 bits (127), Expect(4) = 6e-044
Identities = 19/45 (42%), Positives = 32/45 (71%)
Frame = +2 / +2
Query: 1385 YSGQDFRYLPFGSGRRICPGANFALATMEIMLANLMYHFDWEVPN 1519
+ G +F ++PFGSGRR CPG L +++ +A+L++ F WE+P+
Sbjct: 1391 FKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPD 1525
Score = 42.8 bits (87), Expect(4) = 6e-044
Identities = 19/45 (42%), Positives = 28/45 (62%)
Frame = +2 / +2
Query: 959 TMDNVKSLLMNVFEAAIETSYLVLESAMAELMNHRRVMKKLQAEV 1093
T DN+K+++M+V ET +E AMAELM +KK+Q E+
Sbjct: 983 TKDNIKAIIMDVMFGGTETVASAIEWAMAELMKSPEDLKKVQQEL 1117
>gb|DQ222911.1| Medicago sativa ferulate 5-hydroxylase (F5H-K1) mRNA, complete cds
Length = 1904
Score = 70.7 bits (148), Expect(5) = 1e-043
Identities = 31/88 (35%), Positives = 50/88 (56%)
Frame = +2 / +1
Query: 281 HNGFLFLRAGAVPTLIVSSPSAAEAVMRTHDHIFASRPWSMASHILRYNTCDVAFSPLGE 460
+ G LR G + + +S AA V++ D+IF++RP ++A L Y+ D+AF+ G
Sbjct: 313 YGGIFHLRMGFLHMVAISDADAARQVLQVQDNIFSNRPATVAIKYLTYDRADMAFAHYGP 492
Query: 461 YWQQTRKLMNTHLLSNKKVYSFRHGREE 544
+W+Q RKL L S K S++ R+E
Sbjct: 493 FWRQMRKLCVMKLFSRKHAESWQSVRDE 576
Score = 65.7 bits (137), Expect(5) = 1e-043
Identities = 21/44 (47%), Positives = 30/44 (68%)
Frame = +2 / +1
Query: 1235 DCQIDGYHIPANTRVLVNGWAIGRDPAVWEKPEEFMPERFMRDG 1366
+ ++GY IP RV++N WAIGRD W++PE F P RF++ G
Sbjct: 1264 EATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLKPG 1395
Score = 61.1 bits (127), Expect(5) = 1e-043
Identities = 19/45 (42%), Positives = 32/45 (71%)
Frame = +2 / +1
Query: 1385 YSGQDFRYLPFGSGRRICPGANFALATMEIMLANLMYHFDWEVPN 1519
+ G +F ++PFGSGRR CPG L +++ +A+L++ F WE+P+
Sbjct: 1405 FKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPD 1539
Score = 40.9 bits (83), Expect(5) = 1e-043
Identities = 18/45 (40%), Positives = 27/45 (60%)
Frame = +2 / +1
Query: 959 TMDNVKSLLMNVFEAAIETSYLVLESAMAELMNHRRVMKKLQAEV 1093
T DN+K+++M+V ET +E MAELM +KK+Q E+
Sbjct: 997 TKDNIKAIIMDVMFGGTETVASAIEWVMAELMKSPEDLKKVQQEL 1131
Score = 22.1 bits (42), Expect(5) = 1e-043
Identities = 9/24 (37%), Positives = 13/24 (54%)
Frame = +2 / +1
Query: 209 IVGHLHHIGPQTHISLQELVAKYG 280
I+G++ + TH L L KYG
Sbjct: 247 IIGNMLMMDQLTHRGLANLAKKYG 318
>gb|L11046.1|ALFEICA4HA Medicago sativa elicitor-inducible cinnamic acid 4-hydroxylase mRNA,
complete cds
Length = 1744
Score = 84.5 bits (178), Expect(4) = 1e-043
Identities = 33/79 (41%), Positives = 49/79 (62%)
Frame = +2 / +3
Query: 1127 IREDDLSSLPYLKASMKEALRXXXXXXXXXXXYSTADCQIDGYHIPANTRVLVNGWAIGR 1306
+ E DL LPYL+A +KE LR + D +++G+ IPA +++LVN W +
Sbjct: 1068 VTEPDLHKLPYLQAVIKETLRLRMAIPLLVPHMNLHDPKLNGFDIPAESKILVNAWWLPN 1247
Query: 1307 DPAVWEKPEEFMPERFMRD 1363
+PA W+KPEEF PERF+ +
Sbjct: 1248 NPAHWKKPEEFRPERFLEE 1304
Score = 64.8 bits (135), Expect(4) = 1e-043
Identities = 32/98 (32%), Positives = 52/98 (53%)
Frame = +2 / +3
Query: 299 LRAGAVPTLIVSSPSAAEAVMRTHDHIFASRPWSMASHILRYNTCDVAFSPLGEYWQQTR 478
LR G ++VSSP A+ V+ T F SR ++ I D+ F+ GE+W++ R
Sbjct: 240 LRMGQRNLVVVSSPELAKEVLHTQCVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMR 419
Query: 479 KLMNTHLLSNKKVYSFRHGREEEVCLVVDNLREAAAKS 592
++M +NK V +R+G E E VV++++ A S
Sbjct: 420 RIMTVPFFTNKVVQQYRYGWESEAESVVNDVKNNAEAS 533
Score = 49.2 bits (101), Expect(4) = 1e-043
Identities = 19/39 (48%), Positives = 25/39 (64%)
Frame = +2 / +3
Query: 1388 SGQDFRYLPFGSGRRICPGANFALATMEIMLANLMYHFD 1504
+G DFRYLPFG GRR CPG AL + I + L+ + +
Sbjct: 1323 NGNDFRYLPFGVGRRSCPGIILALPILGITIGRLVQNVE 1439
Score = 41.8 bits (85), Expect(4) = 1e-043
Identities = 20/58 (34%), Positives = 34/58 (58%)
Frame = +2 / +3
Query: 920 IDIFLSRYEEYGFTMDNVKSLLMNVFEAAIETSYLVLESAMAELMNHRRVMKKLQAEV 1093
ID L ++ DNV ++ N+ AAIET+ +E +AEL+NH+ + K++ E+
Sbjct: 867 IDHILDAQKKGEINDDNVLYIVENINVAAIETTLWSIEWGIAELVNHQDIQNKVREEM 1040
>gb|AF195800.1|AF195800 Medicago sativa isoflavone synthase 1 (ifs1) mRNA, partial cds
Length = 1501
Score = 70.3 bits (147), Expect(4) = 1e-028
Identities = 24/46 (52%), Positives = 31/46 (67%)
Frame = +2 / +3
Query: 1229 TADCQIDGYHIPANTRVLVNGWAIGRDPAVWEKPEEFMPERFMRDG 1366
T +C+I+GY IP VL N W +GRDP W++P EF PERF+ G
Sbjct: 1089 TEECEINGYVIPEGALVLFNVWQVGRDPKYWDRPSEFRPERFLETG 1226
Score = 58.3 bits (121), Expect(4) = 1e-028
Identities = 23/41 (56%), Positives = 30/41 (73%)
Frame = +2 / +3
Query: 1391 GQDFRYLPFGSGRRICPGANFALATMEIMLANLMYHFDWEV 1513
GQ F+ LPFGSGRR+CPG N A + M +LA+L+ FD +V
Sbjct: 1260 GQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQV 1382
Score = 31.8 bits (63), Expect(4) = 1e-028
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +2 / +3
Query: 437 VAFSPLGEYWQQTRKLMNTHLLSNKKVYSFRHGREEEV 550
VA P G YW+ RKL+ LL+ V R R +++
Sbjct: 315 VAMVPFGPYWRFVRKLIMNDLLNATTVNKLRPLRTQQI 428
Score = 28.1 bits (55), Expect(4) = 1e-028
Identities = 9/31 (29%), Positives = 18/31 (58%)
Frame = +2 / +3
Query: 281 HNGFLFLRAGAVPTLIVSSPSAAEAVMRTHD 373
H L G++PT++ S+P + ++TH+
Sbjct: 159 HGPLFSLSFGSMPTVVASTPELFKLFLQTHE 251
>gb|AF195802.1|AF195802 Medicago sativa isoflavone synthase 3 (ifs3) mRNA, partial cds
Length = 1501
Score = 69.8 bits (146), Expect(4) = 2e-028
Identities = 23/46 (50%), Positives = 31/46 (67%)
Frame = +2 / +3
Query: 1229 TADCQIDGYHIPANTRVLVNGWAIGRDPAVWEKPEEFMPERFMRDG 1366
T +C+I+GY IP +L N W +GRDP W++P EF PERF+ G
Sbjct: 1089 TEECEINGYVIPEGALILFNVWQVGRDPKYWDRPSEFRPERFLETG 1226
Score = 58.3 bits (121), Expect(4) = 2e-028
Identities = 23/41 (56%), Positives = 30/41 (73%)
Frame = +2 / +3
Query: 1391 GQDFRYLPFGSGRRICPGANFALATMEIMLANLMYHFDWEV 1513
GQ F+ LPFGSGRR+CPG N A + M +LA+L+ FD +V
Sbjct: 1260 GQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQV 1382
Score = 31.8 bits (63), Expect(4) = 2e-028
Identities = 14/38 (36%), Positives = 20/38 (52%)
Frame = +2 / +3
Query: 437 VAFSPLGEYWQQTRKLMNTHLLSNKKVYSFRHGREEEV 550
VA P G YW+ RKL+ LL+ V R R +++
Sbjct: 315 VAMVPFGPYWKFVRKLIMNDLLNATTVNKLRPLRTQQI 428
Score = 27.6 bits (54), Expect(4) = 2e-028
Identities = 9/31 (29%), Positives = 18/31 (58%)
Frame = +2 / +3
Query: 281 HNGFLFLRAGAVPTLIVSSPSAAEAVMRTHD 373
H L G++PT++ S+P + ++TH+
Sbjct: 159 HGPLFSLYFGSMPTVVASTPELFKLFLQTHE 251
>gb|AF195801.1|AF195801 Medicago sativa isoflavone synthase 2 (ifs2) mRNA, partial cds
Length = 1501
Score = 66.1 bits (138), Expect(4) = 9e-028
Identities = 22/46 (47%), Positives = 30/46 (65%)
Frame = +2 / +3
Query: 1229 TADCQIDGYHIPANTRVLVNGWAIGRDPAVWEKPEEFMPERFMRDG 1366
T +C+I+GY IP +L N W +GRD W++P EF PERF+ G
Sbjct: 1089 TEECEINGYVIPEGALILFNVWQVGRDSKYWDRPSEFRPERFLETG 1226
Score = 58.3 bits (121), Expect(4) = 9e-028
Identities = 23/41 (56%), Positives = 30/41 (73%)
Frame = +2 / +3
Query: 1391 GQDFRYLPFGSGRRICPGANFALATMEIMLANLMYHFDWEV 1513
GQ F+ LPFGSGRR+CPG N A + M +LA+L+ FD +V
Sbjct: 1260 GQHFQLLPFGSGRRMCPGVNLATSGMATLLASLIQCFDLQV 1382
Score = 33.1 bits (66), Expect(4) = 9e-028
Identities = 14/38 (36%), Positives = 21/38 (55%)
Frame = +2 / +3
Query: 437 VAFSPLGEYWQQTRKLMNTHLLSNKKVYSFRHGREEEV 550
VA +P G YW+ RKL+ LL+ V R R +++
Sbjct: 315 VAMAPFGPYWKFVRKLIMNDLLNATTVNKLRPLRTQQI 428
Score = 27.6 bits (54), Expect(4) = 9e-028
Identities = 9/31 (29%), Positives = 18/31 (58%)
Frame = +2 / +3
Query: 281 HNGFLFLRAGAVPTLIVSSPSAAEAVMRTHD 373
H L G++PT++ S+P + ++TH+
Sbjct: 159 HGPLFSLYFGSMPTVVASTPELFKLFLQTHE 251
>gb|CO512107.1|CO512107 s13dSG18H1200096_108534 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 495
Score = 65.7 bits (137), Expect(2) = 5e-024
Identities = 21/44 (47%), Positives = 30/44 (68%)
Frame = +2 / +2
Query: 1235 DCQIDGYHIPANTRVLVNGWAIGRDPAVWEKPEEFMPERFMRDG 1366
+ ++GY IP RV++N WAIGRD W++PE F P RF++ G
Sbjct: 110 EATVNGYFIPKQARVMINAWAIGRDANCWDEPESFKPSRFLKPG 241
Score = 61.1 bits (127), Expect(2) = 5e-024
Identities = 19/45 (42%), Positives = 32/45 (71%)
Frame = +2 / +2
Query: 1385 YSGQDFRYLPFGSGRRICPGANFALATMEIMLANLMYHFDWEVPN 1519
+ G +F ++PFGSGRR CPG L +++ +A+L++ F WE+P+
Sbjct: 251 FKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELPD 385
>gb|CO516169.1|CO516169 s13dSG67C0300018_445666 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 506
Score = 91.8 bits (194), Expect(2) = 1e-021
Identities = 41/97 (42%), Positives = 52/97 (53%)
Frame = +2 / +2
Query: 1082 QAEVRAYGAEKKLDMIREDDLSSLPYLKASMKEALRXXXXXXXXXXXYSTADCQIDGYHI 1261
+A+V K + E +L L YLK+ +KE +R S CQI+GY I
Sbjct: 176 KAQVEVRRVFDKKGYVDETELHQLIYLKSVIKETMRLHPSLPLLIPRESIKPCQINGYDI 355
Query: 1262 PANTRVLVNGWAIGRDPAVWEKPEEFMPERFMRDGWD 1372
PA TRV+VN WAIGRDP W E F PERF+ +D
Sbjct: 356 PAKTRVIVNAWAIGRDPRYWVDAESFKPERFLNSRFD 466
Score = 26.7 bits (52), Expect(2) = 1e-021
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +2 / +2
Query: 1385 YSGQDFRYLPFGS 1423
+ G DF Y+PFGS
Sbjct: 467 FKGTDFEYIPFGS 505
>gb|CO513758.1|CO513758 s13dSG15G1200088_140298 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 473
Score = 63.8 bits (133), Expect(3) = 8e-021
Identities = 21/44 (47%), Positives = 33/44 (75%)
Frame = +2 / +1
Query: 1382 SYSGQDFRYLPFGSGRRICPGANFALATMEIMLANLMYHFDWEV 1513
+Y G +F +PFG+GRRICPG A TM +M+A+L+++F+W +
Sbjct: 76 NYKGNNFELIPFGAGRRICPGLPLAHRTMHLMVASLLHNFEWNL 207
Score = 42.3 bits (86), Expect(3) = 8e-021
Identities = 13/20 (65%), Positives = 17/20 (85%)
Frame = +2 / +1
Query: 1298 IGRDPAVWEKPEEFMPERFM 1357
+GRDPA+W+ P F+PERFM
Sbjct: 4 MGRDPAIWDNPNPFVPERFM 63
Score = 27.2 bits (53), Expect(3) = 8e-021
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +2 / +1
Query: 1544 VSMDETFGLMLRRNEPL 1594
++MDE FGL L+R +PL
Sbjct: 232 LNMDEQFGLTLKRVQPL 282
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,964,351
Number of Sequences: 7669
Number of extensions: 146570
Number of successful extensions: 1053
Number of sequences better than 1.0e-020: 9
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 518434108
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)