TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1716207.2.2
(754 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO516263.1|CO516263 s13dSG69D0200014_445854 Glandular tr... 145 5e-036
gb|CO516472.1|CO516472 s13dSG28C1100084_446272 Glandular tr... 145 5e-036
gb|CO516674.1|CO516674 s13dSG74A0600038_446676 Glandular tr... 145 5e-036
gb|CO513029.1|CO513029 s13dSG09G0900068_121984 Glandular tr... 145 7e-036
>gb|CO516263.1|CO516263 s13dSG69D0200014_445854 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 500
Score = 145 bits (312), Expect = 5e-036
Identities = 62/73 (84%), Positives = 68/73 (93%)
Frame = +3 / +3
Query: 177 VNNAVLFDQATYDKLLSEVPKYKQITPSILSERLRINGSLARRAIKDLMARGLIRMVSVH 356
VNN VLFDQ TYDKLL+E PKYK ITPS+LS+RLRINGSLARRAI+DLMA+GLIR+VS H
Sbjct: 192 VNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMAKGLIRLVSAH 371
Query: 357 SSQQIYTRATNT* 395
SSQQIYTRATNT*
Sbjct: 372 SSQQIYTRATNT* 410
Score = 102 bits (218), Expect = 4e-023
Identities = 47/100 (47%), Positives = 65/100 (65%)
Frame = +2 / +2
Query: 95 AAVVEAGQVRWWQAKEEEVEQGKAKGEGKQRGAVRPGYLRQAALGGAQVQANHPFHPLRA 274
++ ++A Q+ WQ +EEEVEQGKAKGE +Q GAV GYL QA Q+QA+H F PL +
Sbjct: 110 SSFLQASQIWRWQTEEEEVEQGKAKGESEQHGAV*SGYL*QALN*STQIQAHHSFCPL*S 289
Query: 275 SEDQRLPCSEGHQGLDGAWPHSDGLGPLKPADLHQGNQYI 394
ED + C EG++ DG + G+ K ADLHQG +++
Sbjct: 290 FED*WITCKEGYKRFDG*RFNQVGVCSFKSADLHQGYKHL 409
Score = 89.5 bits (189), Expect(2) = 8e-026
Identities = 46/71 (64%), Positives = 53/71 (74%)
Frame = -1 / -2
Query: 400 HPYVLVALV*ICWLEWTETIRMRPRAIKSLMALRAREPLILRRSERMEGVICLYLGTSES 221
H V VALV*IC LE +T ++P AIKSL+AL A +PLIL+RSER EGV+ LYLG S
Sbjct: 415 HT*VFVALV*IC*LE*ADTNLIKPLAIKSLIALLASDPLILKRSERTEGVMSLYLGASVK 236
Query: 220 SLS*VAWSNST 188
SLS*V SNST
Sbjct: 235 SLS*VP*SNST 203
Score = 41.8 bits (85), Expect(2) = 8e-026
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = -2 / -3
Query: 174 SPLAFPCSTSSSFACHHRTWPA 109
SP AFPCSTSSS CH + W A
Sbjct: 189 SPFAFPCSTSSSSVCHRQIWLA 124
>gb|CO516472.1|CO516472 s13dSG28C1100084_446272 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 481
Score = 145 bits (312), Expect = 5e-036
Identities = 62/73 (84%), Positives = 68/73 (93%)
Frame = +3 / +2
Query: 177 VNNAVLFDQATYDKLLSEVPKYKQITPSILSERLRINGSLARRAIKDLMARGLIRMVSVH 356
VNN VLFDQ TYDKLL+E PKYK ITPS+LS+RLRINGSLARRAI+DLMA+GLIR+VS H
Sbjct: 176 VNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMAKGLIRLVSAH 355
Query: 357 SSQQIYTRATNT* 395
SSQQIYTRATNT*
Sbjct: 356 SSQQIYTRATNT* 394
Score = 102 bits (217), Expect = 6e-023
Identities = 48/100 (48%), Positives = 65/100 (65%)
Frame = +2 / +1
Query: 95 AAVVEAGQVRWWQAKEEEVEQGKAKGEGKQRGAVRPGYLRQAALGGAQVQANHPFHPLRA 274
++ ++A Q+ WQ +EEEVEQGKAKGE Q GAV GYL QA Q+QA+H F PL +
Sbjct: 94 SSFLQASQIWRWQTEEEEVEQGKAKGES*QHGAV*SGYL*QALN*STQIQAHHSFCPL*S 273
Query: 275 SEDQRLPCSEGHQGLDGAWPHSDGLGPLKPADLHQGNQYI 394
ED + C EG++ LDG + G+ K ADLHQG +++
Sbjct: 274 FED*WITCKEGYKRLDG*RFNQVGVCSFKSADLHQGYKHL 393
Score = 88.1 bits (186), Expect(2) = 2e-025
Identities = 45/68 (66%), Positives = 52/68 (76%)
Frame = -1 / -2
Query: 391 VLVALV*ICWLEWTETIRMRPRAIKSLMALRAREPLILRRSERMEGVICLYLGTSESSLS 212
V VALV*IC LE +T ++P AIKSL+AL A +PLIL+RSER EGV+ LYLG S SLS
Sbjct: 390 VFVALV*IC*LE*ADTNLIKPLAIKSLIALLASDPLILKRSERTEGVMSLYLGASVKSLS 211
Query: 211 *VAWSNST 188
*V SNST
Sbjct: 210 *VP*SNST 187
Score = 41.8 bits (85), Expect(2) = 2e-025
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = -2 / -3
Query: 174 SPLAFPCSTSSSFACHHRTWPA 109
SP AFPCSTSSS CH + W A
Sbjct: 173 SPFAFPCSTSSSSVCHRQIWLA 108
>gb|CO516674.1|CO516674 s13dSG74A0600038_446676 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 488
Score = 145 bits (312), Expect = 5e-036
Identities = 62/73 (84%), Positives = 68/73 (93%)
Frame = +3 / +1
Query: 177 VNNAVLFDQATYDKLLSEVPKYKQITPSILSERLRINGSLARRAIKDLMARGLIRMVSVH 356
VNN VLFDQ TYDKLL+E PKYK ITPS+LS+RLRINGSLARRAI+DLMA+GLIR+VS H
Sbjct: 184 VNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMAKGLIRLVSAH 363
Query: 357 SSQQIYTRATNT* 395
SSQQIYTRATNT*
Sbjct: 364 SSQQIYTRATNT* 402
Score = 102 bits (218), Expect = 4e-023
Identities = 47/100 (47%), Positives = 65/100 (65%)
Frame = +2 / +3
Query: 95 AAVVEAGQVRWWQAKEEEVEQGKAKGEGKQRGAVRPGYLRQAALGGAQVQANHPFHPLRA 274
++ ++A Q+ WQ +EEEVEQGKAKGE +Q GAV GYL QA Q+QA+H F PL +
Sbjct: 102 SSFLQASQIWRWQTEEEEVEQGKAKGESEQHGAV*SGYL*QALN*STQIQAHHSFCPL*S 281
Query: 275 SEDQRLPCSEGHQGLDGAWPHSDGLGPLKPADLHQGNQYI 394
ED + C EG++ DG + G+ K ADLHQG +++
Sbjct: 282 FED*WITCKEGYKRFDG*RFNQVGVCSFKSADLHQGYKHL 401
Score = 89.5 bits (189), Expect(2) = 8e-026
Identities = 46/71 (64%), Positives = 53/71 (74%)
Frame = -1 / -1
Query: 400 HPYVLVALV*ICWLEWTETIRMRPRAIKSLMALRAREPLILRRSERMEGVICLYLGTSES 221
H V VALV*IC LE +T ++P AIKSL+AL A +PLIL+RSER EGV+ LYLG S
Sbjct: 407 HT*VFVALV*IC*LE*ADTNLIKPLAIKSLIALLASDPLILKRSERTEGVMSLYLGASVK 228
Query: 220 SLS*VAWSNST 188
SLS*V SNST
Sbjct: 227 SLS*VP*SNST 195
Score = 41.8 bits (85), Expect(2) = 8e-026
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = -2 / -2
Query: 174 SPLAFPCSTSSSFACHHRTWPA 109
SP AFPCSTSSS CH + W A
Sbjct: 181 SPFAFPCSTSSSSVCHRQIWLA 116
>gb|CO513029.1|CO513029 s13dSG09G0900068_121984 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 545
Score = 145 bits (311), Expect = 7e-036
Identities = 61/72 (84%), Positives = 67/72 (93%)
Frame = +3 / +3
Query: 177 VNNAVLFDQATYDKLLSEVPKYKQITPSILSERLRINGSLARRAIKDLMARGLIRMVSVH 356
VNN VLFDQ TYDKLL+E PKYK ITPS+LS+RLRINGSLARRAI+DLMA+GLIR+VS H
Sbjct: 171 VNNMVLFDQGTYDKLLTEAPKYKLITPSVLSDRLRINGSLARRAIRDLMAKGLIRLVSAH 350
Query: 357 SSQQIYTRATNT 392
SSQQIYTRATNT
Sbjct: 351 SSQQIYTRATNT 386
Score = 99.6 bits (211), Expect = 4e-022
Identities = 47/99 (47%), Positives = 64/99 (64%)
Frame = +2 / +2
Query: 95 AAVVEAGQVRWWQAKEEEVEQGKAKGEGKQRGAVRPGYLRQAALGGAQVQANHPFHPLRA 274
++ ++A Q+ WQ +EEEVEQGKAKGE + GAV GYL QA Q+QA+H F PL +
Sbjct: 89 SSFLQASQIWRWQTEEEEVEQGKAKGESE*HGAV*SGYL*QALN*STQIQAHHSFCPL*S 268
Query: 275 SEDQRLPCSEGHQGLDGAWPHSDGLGPLKPADLHQGNQY 391
ED + C EG++ LDG + G+ K ADLHQG ++
Sbjct: 269 FED*WITCKEGYKRLDG*RFNQVGVCSFKSADLHQGYKH 385
Score = 92.3 bits (195), Expect(2) = 1e-026
Identities = 46/71 (64%), Positives = 54/71 (76%)
Frame = -1 / -2
Query: 400 HPYVLVALV*ICWLEWTETIRMRPRAIKSLMALRAREPLILRRSERMEGVICLYLGTSES 221
H +V VALV*IC LE +T ++P AIKSL+AL A +PLIL+RSER EGV+ LYLG S
Sbjct: 394 HTWVFVALV*IC*LE*ADTNLIKPLAIKSLIALLASDPLILKRSERTEGVMSLYLGASVK 215
Query: 220 SLS*VAWSNST 188
SLS*V SNST
Sbjct: 214 SLS*VP*SNST 182
Score = 41.8 bits (85), Expect(2) = 1e-026
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = -2 / -3
Query: 174 SPLAFPCSTSSSFACHHRTWPA 109
SP AFPCSTSSS CH + W A
Sbjct: 168 SPFAFPCSTSSSSVCHRQIWLA 103
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,306,503
Number of Sequences: 7669
Number of extensions: 68497
Number of successful extensions: 954
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 192705760
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)