TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 131584.2.4
         (739 letters)

Database: MedicagoSativa_nucl_with_EST.fasta 
           7669 sequences; 3,745,706 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AJ410120.1|AJ410120  AJ410120 Medicago sativa library (Ka...   186   3e-048

>gb|AJ410120.1|AJ410120 AJ410120 Medicago sativa library (Kalo P) Medicago sativa cDNA
           clone U40, mRNA sequence
          Length = 757

 Score =  186 bits (400), Expect = 3e-048
 Identities = 75/84 (89%), Positives = 79/84 (94%)
 Frame = +1 / +3

                                                                       
Query: 184 DENQAGKKEEEEFSTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVR 363
           +E+  GK EEEEF+TGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVR
Sbjct: 126 EEDAVGKNEEEEFNTGPLSVLMMSVKNNTQVLINCRNNKKLLGRVRAFDRHCNMVLENVR 305

                                   
Query: 364 EMWTEIPKTGKGKKKALPVNKDRF 435
           EMWTE+PKTGKGKKKA PVNKD F
Sbjct: 306 EMWTEVPKTGKGKKKAQPVNKDPF 377




 Score =  119 bits (254), Expect = 5e-028
 Identities = 55/72 (76%), Positives = 58/72 (80%)
 Frame = -3 / -1

                                                                       
Query: 434 NLSLFTGRAFFLPLPVLGISVHISRTFSSTMLQWRSKARTRPSSFLLLRQLMRTWVLFLT 255
           N SL TG AFF PLPVLG SVHISRTFS T+LQ  SKA TRP SFLLLRQL+RT VLF T
Sbjct: 376 NGSLLTG*AFFFPLPVLGTSVHISRTFSRTILQCLSKALTRPRSFLLLRQLIRTCVLFFT 197

                       
Query: 254 LIIRTDRGPVLN 219
           LI+ TD GPVLN
Sbjct: 196 LIMSTDSGPVLN 161



  Database: MedicagoSativa_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:23 PM
  Number of letters in database: 3,745,706
  Number of sequences in database:  7669
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 6,332,214
Number of Sequences: 7669
Number of extensions: 82290
Number of successful extensions: 1137
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 188073410
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)