TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131554.2.6
(761 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512169.1|CO512169 s13dSG34G0400024_108658 Glandular tr... 179 3e-046
gb|CO514535.1|CO514535 s13dSG43H0500046_327730 Glandular tr... 179 3e-046
gb|CO516654.1|CO516654 s13dSG66G0700054_446636 Glandular tr... 179 3e-046
gb|CO514412.1|CO514412 s13dSG36C0700052_327484 Glandular tr... 177 1e-045
gb|CO515011.1|CO515011 s13dSG26C0700054_397527 Glandular tr... 177 1e-045
gb|AJ888481.1|AJ888481 AJ888481 Medicago sativa subsp. x va... 177 2e-045
gb|CO513556.1|CO513556 s13dSG12H0400032_139894 Glandular tr... 176 3e-045
gb|CO513425.1|CO513425 s13dSG38H1200096_130014 Glandular tr... 176 4e-045
gb|CO512253.1|CO512253 s13dSG03G0900068_114020 Glandular tr... 175 5e-045
gb|CO514208.1|CO514208 s13dSG70F0900075_157078 Glandular tr... 119 4e-028
>gb|CO512169.1|CO512169 s13dSG34G0400024_108658 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 488
Score = 179 bits (386), Expect = 3e-046
Identities = 78/78 (100%), Positives = 78/78 (100%)
Frame = -1 / +2
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT
Sbjct: 125 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 304
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 305 VTAMDVVYALKRQGRTLY 358
Score = 98.7 bits (209), Expect = 8e-022
Identities = 47/66 (71%), Positives = 54/66 (81%)
Frame = +1 / -3
Query: 415 A*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAGLV 594
A*TTS+AVTV ACSV*VTASR+TFS+KI +TPRVSS*+ P +R TPPL A RIAG V
Sbjct: 330 A*TTSIAVTVFLLACSV*VTASRITFSKKIFNTPRVSS*IKPLIRFTPPLRAKRRIAGFV 151
Query: 595 MPWMLS 612
+P MLS
Sbjct: 150 IP*MLS 133
>gb|CO514535.1|CO514535 s13dSG43H0500046_327730 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 504
Score = 179 bits (386), Expect = 3e-046
Identities = 78/78 (100%), Positives = 78/78 (100%)
Frame = -1 / +2
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT
Sbjct: 146 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 325
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 326 VTAMDVVYALKRQGRTLY 379
Score = 105 bits (223), Expect(2) = 3e-025
Identities = 49/66 (74%), Positives = 54/66 (81%)
Frame = +1 / -1
Query: 415 A*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAGLV 594
A*TTSMAVTV ACSV VTASRMTFS+KI +TP VSS*+ P + TPPLLA RIAGLV
Sbjct: 351 A*TTSMAVTVFLLACSVYVTASRMTFSKKIFNTPLVSS*IKPLILFTPPLLAKRRIAGLV 172
Query: 595 MPWMLS 612
+PWMLS
Sbjct: 171 IPWMLS 154
Score = 24.4 bits (47), Expect(2) = 3e-025
Identities = 7/18 (38%), Positives = 15/18 (83%)
Frame = +3 / -3
Query: 378 AEAVEGAALSLERVDDIH 431
++ +EG++L L+ +D+IH
Sbjct: 388 SKPIEGSSLPLQSIDNIH 335
>gb|CO516654.1|CO516654 s13dSG66G0700054_446636 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 465
Score = 179 bits (386), Expect = 3e-046
Identities = 78/78 (100%), Positives = 78/78 (100%)
Frame = -1 / +3
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT
Sbjct: 126 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 305
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 306 VTAMDVVYALKRQGRTLY 359
Score = 98.7 bits (209), Expect = 8e-022
Identities = 47/66 (71%), Positives = 54/66 (81%)
Frame = +1 / -3
Query: 415 A*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAGLV 594
A*TTS+AVTV ACSV*VTASR+TFS+KI +TPRVSS*+ P +R TPPL A RIAG V
Sbjct: 331 A*TTSIAVTVFLLACSV*VTASRITFSKKIFNTPRVSS*IKPLIRFTPPLRAKRRIAGFV 152
Query: 595 MPWMLS 612
+P MLS
Sbjct: 151 IP*MLS 134
>gb|CO514412.1|CO514412 s13dSG36C0700052_327484 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 496
Score = 177 bits (381), Expect = 1e-045
Identities = 77/78 (98%), Positives = 77/78 (98%)
Frame = -1 / +1
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDN QGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT
Sbjct: 229 VLRDNTQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 408
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 409 VTAMDVVYALKRQGRTLY 462
Score = 96.8 bits (205), Expect = 3e-021
Identities = 46/66 (69%), Positives = 54/66 (81%)
Frame = +1 / -3
Query: 415 A*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAGLV 594
A*TTS+AVTV ACSV*VTASR+TFS+KI +TPRVSS*+ P +R TPPL A RIAG V
Sbjct: 434 A*TTSIAVTVFLLACSV*VTASRITFSKKIFNTPRVSS*IKPLIRFTPPLRAKRRIAGFV 255
Query: 595 MPWMLS 612
+P +LS
Sbjct: 254 IP*VLS 237
>gb|CO515011.1|CO515011 s13dSG26C0700054_397527 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 472
Score = 177 bits (381), Expect = 1e-045
Identities = 77/78 (98%), Positives = 77/78 (98%)
Frame = -1 / +1
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDN QGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT
Sbjct: 229 VLRDNTQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 408
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 409 VTAMDVVYALKRQGRTLY 462
Score = 96.8 bits (205), Expect = 3e-021
Identities = 46/66 (69%), Positives = 54/66 (81%)
Frame = +1 / -3
Query: 415 A*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAGLV 594
A*TTS+AVTV ACSV*VTASR+TFS+KI +TPRVSS*+ P +R TPPL A RIAG V
Sbjct: 434 A*TTSIAVTVFLLACSV*VTASRITFSKKIFNTPRVSS*IKPLIRFTPPLRAKRRIAGFV 255
Query: 595 MPWMLS 612
+P +LS
Sbjct: 254 IP*VLS 237
>gb|AJ888481.1|AJ888481 AJ888481 Medicago sativa subsp. x varia 4-day-old Medicago sativa
subsp. x varia cDNA, mRNA sequence
Length = 453
Score = 177 bits (380), Expect = 2e-045
Identities = 77/78 (98%), Positives = 77/78 (98%)
Frame = -1 / +2
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDNIQGITKPAIRRLARRGGVKRISGLIYE TRGVLKIFLENVIRDAVTYTEHARRKT
Sbjct: 137 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEXTRGVLKIFLENVIRDAVTYTEHARRKT 316
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 317 VTAMDVVYALKRQGRTLY 370
Score = 105 bits (225), Expect(2) = 4e-027
Identities = 50/66 (75%), Positives = 53/66 (80%)
Frame = +1 / -1
Query: 415 A*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAGLV 594
A*TTSMAVTV ACSV*VTASR+TFS+KIL TPRV S*M P + TPP LA RIAG V
Sbjct: 342 A*TTSMAVTVFLLACSV*VTASRITFSKKILRTPRVXS*MRPLILFTPPRLARRRIAGFV 163
Query: 595 MPWMLS 612
MPWMLS
Sbjct: 162 MPWMLS 145
Score = 29.9 bits (59), Expect(2) = 4e-027
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +3 / -3
Query: 345 QFATR*GSDLAAEAVEGAALSLERVDDIH 431
+F T+* +L E VE +LSL+ +++IH
Sbjct: 412 RFRTK*TLNLTTETVESPSLSLQSINNIH 326
>gb|CO513556.1|CO513556 s13dSG12H0400032_139894 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 553
Score = 176 bits (379), Expect = 3e-045
Identities = 77/78 (98%), Positives = 77/78 (98%)
Frame = -1 / +3
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDA TYTEHARRKT
Sbjct: 120 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDADTYTEHARRKT 299
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 300 VTAMDVVYALKRQGRTLY 353
Score = 92.3 bits (195), Expect(2) = 5e-022
Identities = 45/68 (66%), Positives = 55/68 (80%)
Frame = +1 / -1
Query: 409 LSA*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAG 588
L A*TTS+AVTV ACSV*V+AS +TFS+KI +TP VSS*++P + TPPLLA+ RIAG
Sbjct: 331 LRA*TTSIAVTVFLLACSV*VSASLITFSKKIFNTPLVSS*INPLILFTPPLLANRRIAG 152
Query: 589 LVMPWMLS 612
V+P MLS
Sbjct: 151 FVIP*MLS 128
Score = 26.3 bits (51), Expect(2) = 5e-022
Identities = 9/22 (40%), Positives = 16/22 (72%)
Frame = +3 / -3
Query: 366 SDLAAEAVEGAALSLERVDDIH 431
S E +EG++LSL+ +++IH
Sbjct: 374 SSSTTETIEGSSLSLKGINNIH 309
>gb|CO513425.1|CO513425 s13dSG38H1200096_130014 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 488
Score = 176 bits (378), Expect = 4e-045
Identities = 77/78 (98%), Positives = 77/78 (98%)
Frame = -1 / +2
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKI LENVIRDAVTYTEHARRKT
Sbjct: 131 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKISLENVIRDAVTYTEHARRKT 310
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAMDVVYALKRQGRTLY
Sbjct: 311 VTAMDVVYALKRQGRTLY 364
Score = 105 bits (225), Expect(2) = 5e-027
Identities = 50/66 (75%), Positives = 54/66 (81%)
Frame = +1 / -3
Query: 415 A*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAGLV 594
A*TTSMAVTV ACSV*VTASR+TFS++IL TPRVSS*M P + TPP LA RIAG V
Sbjct: 336 A*TTSMAVTVFLLACSV*VTASRITFSKEILRTPRVSS*MRPLILFTPPRLARRRIAGFV 157
Query: 595 MPWMLS 612
MPWMLS
Sbjct: 156 MPWMLS 139
Score = 29.5 bits (58), Expect(2) = 5e-027
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = +3 / -2
Query: 345 QFATR*GSDLAAEAVEGAALSLERVDDIH 431
+F T+* +L E VE +LSL+ +++IH
Sbjct: 406 RFRTK*TLNLTTEPVESPSLSLQSINNIH 320
>gb|CO512253.1|CO512253 s13dSG03G0900068_114020 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 563
Score = 175 bits (377), Expect = 5e-045
Identities = 77/78 (98%), Positives = 77/78 (98%)
Frame = -1 / +2
Query: 620 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 441
VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT
Sbjct: 125 VLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKT 304
Query: 440 VTAMDVVYALKRQGRTLY 387
VTAM VVYALKRQGRTLY
Sbjct: 305 VTAMVVVYALKRQGRTLY 358
Score = 97.3 bits (206), Expect = 2e-021
Identities = 46/66 (69%), Positives = 54/66 (81%)
Frame = +1 / -3
Query: 415 A*TTSMAVTVLRRACSV*VTASRMTFSRKILSTPRVSS*MSPEMRLTPPLLASLRIAGLV 594
A*TT++AVTV ACSV*VTASR+TFS+KI +TPRVSS*+ P +R TPPL A RIAG V
Sbjct: 330 A*TTTIAVTVFLLACSV*VTASRITFSKKIFNTPRVSS*IKPLIRFTPPLRAKRRIAGFV 151
Query: 595 MPWMLS 612
+P MLS
Sbjct: 150 IP*MLS 133
>gb|CO514208.1|CO514208 s13dSG70F0900075_157078 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 288
Score = 119 bits (255), Expect = 4e-028
Identities = 52/54 (96%), Positives = 52/54 (96%)
Frame = -1 / +3
Query: 617 LRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEH 456
LRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLEN IRDA TYTEH
Sbjct: 126 LRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENGIRDAGTYTEH 287
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,554,070
Number of Sequences: 7669
Number of extensions: 80597
Number of successful extensions: 4348
Number of sequences better than 1.0e-020: 12
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 195485170
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)