TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.662
(940 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AW698840.1|AW698840 r515 non-glandular-haired subtracted... 172 9e-053
gb|CO513810.1|CO513810 s13dSG82H0100012_156282 Glandular tr... 150 3e-037
gb|CO517170.1|CO517170 s13dSG33E0100007_474424 Glandular tr... 143 3e-035
>gb|AW698840.1|AW698840 r515 non-glandular-haired subtracted cDNA library Medicago sativa
cDNA, mRNA sequence
Length = 397
Score = 172 bits (371), Expect(3) = 9e-053
Identities = 70/76 (92%), Positives = 73/76 (96%)
Frame = -2 / +2
Query: 636 AQAKSRHSNGQGRWPVKSARFILDLLKNAESNADVKGLDVDNLYVSHIQVNQAQKQRRRT 457
AQAK+RHSNGQGRWPVKSA FILDLLKNAESNA+VKGLDVD LYVSHIQVNQAQ+ RRRT
Sbjct: 53 AQAKNRHSNGQGRWPVKSAXFILDLLKNAESNAEVKGLDVDALYVSHIQVNQAQEPRRRT 232
Query: 456 YRAHGRINPYMSSPCH 409
YRAHGRINPYMSSPCH
Sbjct: 233 YRAHGRINPYMSSPCH 280
Score = 110 bits (234), Expect(2) = 4e-027
Identities = 50/77 (64%), Positives = 56/77 (72%)
Frame = +2 / -2
Query: 386 FSDRISSMWQGEDM*GLMRP*AR*VRRLCFWAWFTWMCET*RLSTSKPFTSALLSAFFSK 565
F + + MWQG+DM*GL+ P A V RL A TW+CET R STSKP TSALLSAFFSK
Sbjct: 303 FRIKSTXMWQGDDM*GLILPWALYVLRLGSCA*LTWICETYRASTSKPLTSALLSAFFSK 124
Query: 566 SNMNLADLTGQRPCPLE 616
S +N ADLTG PCPLE
Sbjct: 123 SRINXADLTGHLPCPLE 73
Score = 39.6 bits (80), Expect(3) = 9e-053
Identities = 14/16 (87%), Positives = 15/16 (93%)
Frame = -2 / +3
Query: 687 HKQAIPFRRYCGGVGR 640
HKQAIPFRR+C GVGR
Sbjct: 3 HKQAIPFRRFCRGVGR 50
Score = 28.1 bits (55), Expect(3) = 9e-053
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = -2 / +3
Query: 405 ELILSEKEEPVKKEADNIVAARKQ 334
ELILSEKEE +KE +AA K+
Sbjct: 285 ELILSEKEEAGQKEPGAQLAASKK 356
Score = 25.8 bits (50), Expect(2) = 4e-027
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2 / -1
Query: 341 LAATMLSASFFTGSSFSDRISS 406
LAA+ SF SSFSD+I+S
Sbjct: 349 LAASWAPGSF*PASSFSDKINS 284
>gb|CO513810.1|CO513810 s13dSG82H0100012_156282 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 269
Score = 150 bits (322), Expect = 3e-037
Identities = 61/71 (85%), Positives = 65/71 (91%)
Frame = -2 / +2
Query: 852 AAMVKYSQEPGNPTKSAKAMGRDLRVHFKNTRETAFALRKLPLTKAKRYLEDVIAHKQAI 673
AAMVKYS+EP NPTKS KA G DLRVHFKNTRETAFA+RKLPLTKAKRYLEDV+AHKQA
Sbjct: 56 AAMVKYSKEPDNPTKSTKARGADLRVHFKNTRETAFAIRKLPLTKAKRYLEDVLAHKQAF 235
Query: 672 PFRRYCGGVGR 640
PFRR+C GVGR
Sbjct: 236 PFRRFCRGVGR 268
Score = 97.7 bits (207), Expect = 2e-021
Identities = 47/70 (67%), Positives = 52/70 (74%)
Frame = +2 / -3
Query: 644 PTPPQYLRKGIACL*AITSSRYRLALVKGSLRSAKAVSLVFLKWTLRSLPMALADLVGLP 823
PTP Q KG ACL A TSS+Y LALV+G+ A AVSLVFLK TLRS P+AL DLVGL
Sbjct: 264 PTPRQKRLKGKACLWAKTSSKYLLALVRGNFLMANAVSLVFLK*TLRSAPLALVDLVGLS 85
Query: 824 GSCEYLTIAA 853
GS EY T+AA
Sbjct: 84 GSLEYFTMAA 55
>gb|CO517170.1|CO517170 s13dSG33E0100007_474424 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 558
Score = 143 bits (307), Expect = 3e-035
Identities = 61/73 (83%), Positives = 65/73 (89%)
Frame = -2 / -1
Query: 552 AESNADVKGLDVDNLYVSHIQVNQAQKQRRRTYRAHGRINPYMSSPCHIELILSEKEEPV 373
AESNA+VKGLDVD LYVSHIQVNQAQKQRRRTYRAHGRINPYMS PCHIELILSEKEE V
Sbjct: 558 AESNAEVKGLDVDALYVSHIQVNQAQKQRRRTYRAHGRINPYMSFPCHIELILSEKEEAV 379
Query: 372 KKEADNIVAARKQ 334
KE + +AA K+
Sbjct: 378 MKEPETQLAASKK 340
Score = 101 bits (216), Expect = 1e-022
Identities = 47/70 (67%), Positives = 51/70 (72%)
Frame = +2 / +2
Query: 341 LAATMLSASFFTGSSFSDRISSMWQGEDM*GLMRP*AR*VRRLCFWAWFTWMCET*RLST 520
LAA +S SF T SSFSD I+SMWQG DM*G + P A V RLCF A TW+CET R ST
Sbjct: 347 LAAN*VSGSFITASSFSDNINSMWQGNDM*GFILPWALYVLRLCFCA*LTWICETYRAST 526
Query: 521 SKPFTSALLS 550
SKP TSALLS
Sbjct: 527 SKPLTSALLS 556
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,482,002
Number of Sequences: 7669
Number of extensions: 116896
Number of successful extensions: 1150
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 247157469
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)