TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.654
(682 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512871.1|CO512871 s13dSG20G0100004_121668 Glandular tr... 241 9e-065
gb|CO513398.1|CO513398 s13dSG38E1200087_129960 Glandular tr... 225 4e-060
gb|CO515891.1|CO515891 s13dSG91D0900076_421321 Glandular tr... 225 4e-060
gb|CO514195.1|CO514195 s13dSG70E0500035_157052 Glandular tr... 213 2e-056
gb|CO517225.1|CO517225 s13dSG32C0500038_486046 Glandular tr... 148 9e-037
>gb|CO512871.1|CO512871 s13dSG20G0100004_121668 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 441
Score = 241 bits (520), Expect = 9e-065
Identities = 94/127 (74%), Positives = 113/127 (88%)
Frame = -2 / +2
Query: 621 KVYTLEEVAKHNTKDDCWLVIGGKVYNVTKFLEDHPGGDDVLLSSTAKDATDDFEDVGHS 442
KV+TL +V+KHN+ DCWLVI KVY+VTKFLEDHPGGD+VL+SST KDA++DF+D+GHS
Sbjct: 41 KVFTLADVSKHNSAKDCWLVIHNKVYDVTKFLEDHPGGDEVLISSTGKDASNDFDDIGHS 220
Query: 441 TTARAMMDEYLVGEIDAATIPSKVKYTPPKQPHYNQDKTPEFVIKILQFLVPLAILGLAV 262
T+A MM+E+ VG+ID++TIPSKV YTPPKQPHYNQDKT EFVIKILQFLVPL ILG+AV
Sbjct: 221 TSAYTMMEEFYVGDIDSSTIPSKVDYTPPKQPHYNQDKTSEFVIKILQFLVPLFILGVAV 400
Query: 261 AVRIYTK 241
+R YTK
Sbjct: 401 GIRFYTK 421
Score = 151 bits (324), Expect = 9e-038
Identities = 78/126 (61%), Positives = 89/126 (70%)
Frame = +2 / -1
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIVAASISPTRYSS 421
LV* TA P I +GT+NWRILMTNS VLS L* GC GGV T G+V S+SPT SS
Sbjct: 420 LV*KRIPTATPKINKGTRNWRILMTNSDVLS*L**GCFGGV*STFEGMVDESMSPT*NSS 241
Query: 422 IIARAVVLCPTSSKSSVASLAVEDSRTSSPPGWSSRNLVTLYTLPPMTSQQSSLVLCLAT 601
II A+VL P SSKS AS VE+ RTSSPPGWSS+N VT TL +TSQQS +LCL T
Sbjct: 240 IIV*ALVL*PISSKSLDASFPVEEIRTSSPPGWSSKNFVTS*TLL*ITSQQSLALLCLET 61
Query: 602 SSRVYT 619
S++V T
Sbjct: 60 SAKVNT 43
>gb|CO513398.1|CO513398 s13dSG38E1200087_129960 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 537
Score = 225 bits (486), Expect = 4e-060
Identities = 89/139 (64%), Positives = 110/139 (79%)
Frame = -2 / +3
Query: 657 DQRKRKRRMSGSKVYTLEEVAKHNTKDDCWLVIGGKVYNVTKFLEDHPGGDDVLLSSTAK 478
D++ K+ S K++T ++V+KHN DCWL++ GKVY+VT F+EDHPGGD+VLLS+T K
Sbjct: 72 DRKCEKKMASDPKLHTFDDVSKHNKTKDCWLILSGKVYDVTPFMEDHPGGDEVLLSATGK 251
Query: 477 DATDDFEDVGHSTTARAMMDEYLVGEIDAATIPSKVKYTPPKQPHYNQDKTPEFVIKILQ 298
DAT+DFEDVGHS +AR MMD+Y +GEID +T+P K Y PP+Q YN DKT EFVIKILQ
Sbjct: 252 DATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKRTYVPPQQSQYNPDKTSEFVIKILQ 431
Query: 297 FLVPLAILGLAVAVRIYTK 241
FLVPL ILGLA VR YTK
Sbjct: 432 FLVPLLILGLAFVVRNYTK 488
Score = 142 bits (305), Expect = 4e-035
Identities = 73/134 (54%), Positives = 87/134 (64%)
Frame = +2 / -3
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIVAASISPTRYSS 421
LV* LT A+P+I RGT+N +IL+TNS VLS L C GG G V SISP Y S
Sbjct: 487 LV*FLTTKAKPNIRRGTRNCKILITNSEVLSGLY*DC*GGT*VRFRGTVDGSISPM*YLS 308
Query: 422 IIARAVVLCPTSSKSSVASLAVEDSRTSSPPGWSSRNLVTLYTLPPMTSQQSSLVLCLAT 601
II+ A LCPTSSKS VAS V D++TSSPPG SS VT YT P SQQS ++LC T
Sbjct: 307 IISLAESLCPTSSKSLVASFPVADNKTSSPPG*SSIKGVTSYTFPERISQQSLVLLCFDT 128
Query: 602 SSRVYTFDPDILLF 643
SS+V +F + + F
Sbjct: 127 SSKV*SFGSEAIFF 86
>gb|CO515891.1|CO515891 s13dSG91D0900076_421321 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 552
Score = 225 bits (486), Expect = 4e-060
Identities = 89/136 (65%), Positives = 109/136 (80%)
Frame = -2 / +3
Query: 648 KRKRRMSGSKVYTLEEVAKHNTKDDCWLVIGGKVYNVTKFLEDHPGGDDVLLSSTAKDAT 469
+RK+ S K++T ++V+KHN DCWL++ GKVY+VT F+EDHPGGD+VLLS+T KDAT
Sbjct: 84 ERKKMASDPKLHTFDDVSKHNKTKDCWLILSGKVYDVTPFMEDHPGGDEVLLSATGKDAT 263
Query: 468 DDFEDVGHSTTARAMMDEYLVGEIDAATIPSKVKYTPPKQPHYNQDKTPEFVIKILQFLV 289
+DFEDVGHS +AR MMD+Y +GEID +T+P K Y PP+Q YN DKT EFVIKILQFLV
Sbjct: 264 NDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKRTYVPPQQSQYNPDKTSEFVIKILQFLV 443
Query: 288 PLAILGLAVAVRIYTK 241
PL ILGLA VR YTK
Sbjct: 444 PLLILGLAFVVRNYTK 491
Score = 147 bits (316), Expect = 1e-036
Identities = 76/135 (56%), Positives = 89/135 (65%)
Frame = +2 / -3
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIVAASISPTRYSS 421
LV* LT A+P+I RGT+N +IL+TNS VLS L C GG L G V SISP Y S
Sbjct: 490 LV*FLTTKAKPNIRRGTRNCKILITNSEVLSGLY*DC*GGT*VRLRGTVDGSISPM*YLS 311
Query: 422 IIARAVVLCPTSSKSSVASLAVEDSRTSSPPGWSSRNLVTLYTLPPMTSQQSSLVLCLAT 601
II+ A LCPTSSKS VAS V D+RTSSPPG SS VT YT P SQQS ++LC T
Sbjct: 310 IISLAESLCPTSSKSLVASFPVADNRTSSPPG*SSIKGVTSYTFPERISQQSLVLLCFDT 131
Query: 602 SSRVYTFDPDILLFL 646
SS+V +F + + FL
Sbjct: 130 SSKV*SFGSEAIFFL 86
>gb|CO514195.1|CO514195 s13dSG70E0500035_157052 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 518
Score = 213 bits (459), Expect = 2e-056
Identities = 86/136 (63%), Positives = 104/136 (76%)
Frame = -2 / +3
Query: 648 KRKRRMSGSKVYTLEEVAKHNTKDDCWLVIGGKVYNVTKFLEDHPGGDDVLLSSTAKDAT 469
+RK+ S K++T ++V KHN DCWL++ GKVY+VT F+EDHPGGD+ LLS+T KDAT
Sbjct: 75 ERKKMASDPKLHTFDDVLKHNKTKDCWLILSGKVYDVTPFMEDHPGGDEGLLSATGKDAT 254
Query: 468 DDFEDVGHSTTARAMMDEYLVGEIDAATIPSKVKYTPPKQPHYNQDKTPEFVIKILQFLV 289
+DFEDVGHS +AR MMD+Y +GEID +T P K Y PP+Q YN DKT EFVIKILQFLV
Sbjct: 255 NDFEDVGHSDSAREMMDKYYIGEIDPSTGPLKRTYVPPQQSQYNPDKTSEFVIKILQFLV 434
Query: 288 PLAILGLAVAVRIYTK 241
PL ILGL R YTK
Sbjct: 435 PLLILGLXFVGRNYTK 482
Score = 136 bits (291), Expect = 3e-033
Identities = 71/135 (52%), Positives = 85/135 (62%)
Frame = +2 / -2
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIVAASISPTRYSS 421
LV* L +P+I RGT+N +IL+TNS VLS L C GG G V SISP Y S
Sbjct: 481 LV*FLPTKXKPNIRRGTRNCKILITNSEVLSGLY*DC*GGT*VRFRGPVDGSISPM*YLS 302
Query: 422 IIARAVVLCPTSSKSSVASLAVEDSRTSSPPGWSSRNLVTLYTLPPMTSQQSSLVLCLAT 601
II+ A LCPTSSKS VAS V D++ SSPPG SS VT YT P SQQS ++LC T
Sbjct: 301 IISLAESLCPTSSKSLVASFPVADNKPSSPPG*SSIKGVTSYTFPERISQQSLVLLCFNT 122
Query: 602 SSRVYTFDPDILLFL 646
SS+V +F + + FL
Sbjct: 121 SSKV*SFGSEAIFFL 77
>gb|CO517225.1|CO517225 s13dSG32C0500038_486046 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 579
Score = 148 bits (317), Expect = 9e-037
Identities = 62/88 (70%), Positives = 71/88 (80%)
Frame = -2 / -1
Query: 504 DVLLSSTAKDATDDFEDVGHSTTARAMMDEYLVGEIDAATIPSKVKYTPPKQPHYNQDKT 325
+VLLS+T KDAT+DFEDVGHS +AR MMD+Y +GEID +T+P K Y PP+Q YN DKT
Sbjct: 552 EVLLSATGKDATNDFEDVGHSDSAREMMDKYYIGEIDPSTVPLKRTYVPPQQSQYNPDKT 373
Query: 324 PEFVIKILQFLVPLAILGLAVAVRIYTK 241
EFVIKILQFLVPL ILGLA VR YTK
Sbjct: 372 SEFVIKILQFLVPLLILGLAFVVRNYTK 289
Score = 99.1 bits (210), Expect = 5e-022
Identities = 52/88 (59%), Positives = 59/88 (67%)
Frame = +2 / +2
Query: 242 LV*ILTATARPSIARGTKNWRILMTNSGVLSWL*CGCLGGVYFTLLGIVAASISPTRYSS 421
LV* LT A+P+I RGT+N +IL+TNS VLS L C GG L G V SISP Y S
Sbjct: 290 LV*FLTTKAKPNIRRGTRNCKILITNSEVLSGLY*DC*GGT*VRLRGTVDGSISPM*YLS 469
Query: 422 IIARAVVLCPTSSKSSVASLAVEDSRTS 505
II+ A LCPTSSKS VAS V D+RTS
Sbjct: 470 IISLAESLCPTSSKSLVASFPVADNRTS 553
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,174,090
Number of Sequences: 7669
Number of extensions: 90621
Number of successful extensions: 1512
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 170470480
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)