TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.593
(1027 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA... 187 1e-096
gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA... 187 1e-096
gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, comple... 187 1e-082
gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular tr... 69 3e-022
>gb|U83592.1|MSU83592 Medicago sativa class I chitinase mRNA, complete cds
Length = 1267
Score = 187 bits (403), Expect(4) = 1e-096
Identities = 68/98 (69%), Positives = 84/98 (85%)
Frame = +3 / +2
Query: 390 YYGRGPIQLTHKYNYRLAGQALNLNLVGDPDLVARDPVVAFKTAIWFWMTPQSPKPSCHA 569
YYGRGPIQ++ YNY G+A+ ++L+ +PDLVA DPV++FKTA+WFWMTPQSPKPSCH
Sbjct: 572 YYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHD 751
Query: 570 VMTGDWTPSATDRAAGRLPGYGLTSNIINGGLECGKGQ 683
V+TG W+PS+ DRAAGRL GYG +NIINGGLECG+GQ
Sbjct: 752 VITGRWSPSSADRAAGRLSGYGTVTNIINGGLECGRGQ 865
Score = 125 bits (268), Expect(4) = 1e-096
Identities = 46/62 (74%), Positives = 51/62 (82%)
Frame = +3 / +2
Query: 186 YTYDAFIEAASKFPGFGTTGDEQTRRRELAAFFGQTSHETTGGWATAPDGPFAWGYCRVK 365
YTYDAFI AA FP F GD T++RE+AAF GQTSHETTGGWATAPDGP+AWGYC V+
Sbjct: 329 YTYDAFISAAKAFPNFANNGDTATKKREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVR 508
Query: 366 EQ 371
EQ
Sbjct: 509 EQ 514
Score = 114 bits (243), Expect(3) = 6e-050
Identities = 47/68 (69%), Positives = 52/68 (76%)
Frame = -1 / -2
Query: 679 PLPHSSPPLMMFEVRPYPGSLPAARSVADGVQSPVITAWHDGLGDCGVIQNQMAVLKATT 500
PLPHSSPP +M PYP SLPAARS +G+ PVIT+W DGLGD GVIQN AVLK T
Sbjct: 861 PLPHSSPPFIMLVTVPYPDSLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDIT 682
Query: 499 GSLATRSG 476
GS+ATRSG
Sbjct: 681 GSVATRSG 658
Score = 84.9 bits (179), Expect(3) = 6e-050
Identities = 37/53 (69%), Positives = 40/53 (75%)
Frame = -1 / -2
Query: 373 FCSFTRQYPQANGPSGAVAHPPVVSWDVWPKKAASSRLRVCSSPVVPKPGNLL 215
FCS T+QYPQA GPSGAVAHPPVVS DV PKKAA S V SP++ K GN L
Sbjct: 516 FCSLTKQYPQAYGPSGAVAHPPVVSCDV*PKKAAISLFLVAVSPLLAKLGNAL 358
Score = 54.7 bits (113), Expect(4) = 1e-096
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = +3 / +2
Query: 699 KDRVGYYKRYCDMLRVGYGDNVPCKDQKP 785
+DR+G+YKRYCD+L VGYG N+ C Q+P
Sbjct: 878 QDRIGFYKRYCDILGVGYGANLDCFSQRP 964
Score = 38.6 bits (78), Expect(4) = 1e-096
Identities = 14/22 (63%), Positives = 17/22 (77%)
Frame = +3 / +2
Query: 114 SIITRPMFQSMLSHRGDSGCQG 179
S+I+R F +ML HR DSGCQG
Sbjct: 254 SLISRDTFNNMLKHRDDSGCQG 319
Score = 38.2 bits (77), Expect(3) = 6e-050
Identities = 18/30 (60%), Positives = 20/30 (66%)
Frame = -1 / -2
Query: 790 P*GFWSLQGTLSPYPTRSISQYLL**PTRS 701
P G Q L+PYPT S+SQYLL* P RS
Sbjct: 969 PKGL*EKQSRLAPYPTPSMSQYLL*NPIRS 880
>gb|U83591.1|MSU83591 Medicago sativa class I chitinase mRNA, complete cds
Length = 1259
Score = 187 bits (403), Expect(4) = 1e-096
Identities = 68/98 (69%), Positives = 84/98 (85%)
Frame = +3 / +1
Query: 390 YYGRGPIQLTHKYNYRLAGQALNLNLVGDPDLVARDPVVAFKTAIWFWMTPQSPKPSCHA 569
YYGRGPIQ++ YNY G+A+ ++L+ +PDLVA DPV++FKTA+WFWMTPQSPKPSCH
Sbjct: 571 YYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHD 750
Query: 570 VMTGDWTPSATDRAAGRLPGYGLTSNIINGGLECGKGQ 683
V+TG W+PS+ DRAAGRL GYG +NIINGGLECG+GQ
Sbjct: 751 VITGRWSPSSADRAAGRLSGYGTVTNIINGGLECGRGQ 864
Score = 125 bits (268), Expect(4) = 1e-096
Identities = 46/62 (74%), Positives = 51/62 (82%)
Frame = +3 / +1
Query: 186 YTYDAFIEAASKFPGFGTTGDEQTRRRELAAFFGQTSHETTGGWATAPDGPFAWGYCRVK 365
YTYDAFI AA FP F GD T++RE+AAF GQTSHETTGGWATAPDGP+AWGYC V+
Sbjct: 328 YTYDAFISAAKAFPNFANNGDTATKKREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVR 507
Query: 366 EQ 371
EQ
Sbjct: 508 EQ 513
Score = 114 bits (243), Expect(3) = 5e-050
Identities = 47/68 (69%), Positives = 52/68 (76%)
Frame = -1 / -1
Query: 679 PLPHSSPPLMMFEVRPYPGSLPAARSVADGVQSPVITAWHDGLGDCGVIQNQMAVLKATT 500
PLPHSSPP +M PYP SLPAARS +G+ PVIT+W DGLGD GVIQN AVLK T
Sbjct: 860 PLPHSSPPFIMLVTVPYPDSLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDIT 681
Query: 499 GSLATRSG 476
GS+ATRSG
Sbjct: 680 GSVATRSG 657
Score = 84.9 bits (179), Expect(3) = 5e-050
Identities = 37/53 (69%), Positives = 40/53 (75%)
Frame = -1 / -1
Query: 373 FCSFTRQYPQANGPSGAVAHPPVVSWDVWPKKAASSRLRVCSSPVVPKPGNLL 215
FCS T+QYPQA GPSGAVAHPPVVS DV PKKAA S V SP++ K GN L
Sbjct: 515 FCSLTKQYPQAYGPSGAVAHPPVVSCDV*PKKAAISLFLVAVSPLLAKLGNAL 357
Score = 54.7 bits (113), Expect(4) = 1e-096
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = +3 / +1
Query: 699 KDRVGYYKRYCDMLRVGYGDNVPCKDQKP 785
+DR+G+YKRYCD+L VGYG N+ C Q+P
Sbjct: 877 QDRIGFYKRYCDILGVGYGANLDCFSQRP 963
Score = 38.6 bits (78), Expect(4) = 1e-096
Identities = 14/22 (63%), Positives = 17/22 (77%)
Frame = +3 / +1
Query: 114 SIITRPMFQSMLSHRGDSGCQG 179
S+I+R F +ML HR DSGCQG
Sbjct: 253 SLISRDTFNNMLKHRDDSGCQG 318
Score = 38.2 bits (77), Expect(3) = 5e-050
Identities = 18/30 (60%), Positives = 20/30 (66%)
Frame = -1 / -1
Query: 790 P*GFWSLQGTLSPYPTRSISQYLL**PTRS 701
P G Q L+PYPT S+SQYLL* P RS
Sbjct: 968 PKGL*EKQSRLAPYPTPSMSQYLL*NPIRS 879
>gb|AY804254.1| Medicago sativa chitinase (Chi) mRNA, complete cds
Length = 987
Score = 187 bits (403), Expect(5) = 1e-082
Identities = 68/98 (69%), Positives = 84/98 (85%)
Frame = +3 / +1
Query: 390 YYGRGPIQLTHKYNYRLAGQALNLNLVGDPDLVARDPVVAFKTAIWFWMTPQSPKPSCHA 569
YYGRGPIQ++ YNY G+A+ ++L+ +PDLVA DPV++FKTA+WFWMTPQSPKPSCH
Sbjct: 541 YYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPKPSCHD 720
Query: 570 VMTGDWTPSATDRAAGRLPGYGLTSNIINGGLECGKGQ 683
V+TG W+PS+ DRAAGRL GYG +NIINGGLECG+GQ
Sbjct: 721 VITGRWSPSSADRAAGRLSGYGTVTNIINGGLECGRGQ 834
Score = 114 bits (243), Expect(3) = 2e-038
Identities = 47/68 (69%), Positives = 52/68 (76%)
Frame = -1 / -2
Query: 679 PLPHSSPPLMMFEVRPYPGSLPAARSVADGVQSPVITAWHDGLGDCGVIQNQMAVLKATT 500
PLPHSSPP +M PYP SLPAARS +G+ PVIT+W DGLGD GVIQN AVLK T
Sbjct: 830 PLPHSSPPFIMLVTVPYPDSLPAARSALEGLHLPVITSWQDGLGDWGVIQNHRAVLKDIT 651
Query: 499 GSLATRSG 476
GS+ATRSG
Sbjct: 650 GSVATRSG 627
Score = 54.7 bits (113), Expect(5) = 1e-082
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = +3 / +1
Query: 699 KDRVGYYKRYCDMLRVGYGDNVPCKDQKP 785
+DR+G+YKRYCD+L VGYG N+ C Q+P
Sbjct: 847 QDRIGFYKRYCDILGVGYGANLDCFSQRP 933
Score = 49.6 bits (102), Expect(5) = 1e-082
Identities = 19/34 (55%), Positives = 21/34 (61%)
Frame = +3 / +1
Query: 186 YTYDAFIEAASKFPGFGTTGDEQTRRRELAAFFG 287
YTYDAFI AA FP F GD T++RE F G
Sbjct: 295 YTYDAFISAAKAFPNFANNGDTATKKREDCCFLG 396
Score = 46.9 bits (96), Expect(5) = 1e-082
Identities = 16/20 (80%), Positives = 18/20 (90%)
Frame = +3 / +1
Query: 312 GWATAPDGPFAWGYCRVKEQ 371
G ATAPDGP+AWGYC V+EQ
Sbjct: 424 GGATAPDGPYAWGYCFVREQ 483
Score = 45.1 bits (92), Expect(3) = 2e-038
Identities = 17/21 (80%), Positives = 18/21 (85%)
Frame = -1 / -2
Query: 373 FCSFTRQYPQANGPSGAVAHP 311
FCS T+QYPQA GPSGAVA P
Sbjct: 485 FCSLTKQYPQAYGPSGAVAPP 423
Score = 38.6 bits (78), Expect(5) = 1e-082
Identities = 14/22 (63%), Positives = 17/22 (77%)
Frame = +3 / +1
Query: 114 SIITRPMFQSMLSHRGDSGCQG 179
S+I+R F +ML HR DSGCQG
Sbjct: 220 SLISRDTFNNMLKHRDDSGCQG 285
Score = 38.2 bits (77), Expect(3) = 2e-038
Identities = 18/30 (60%), Positives = 20/30 (66%)
Frame = -1 / -2
Query: 790 P*GFWSLQGTLSPYPTRSISQYLL**PTRS 701
P G Q L+PYPT S+SQYLL* P RS
Sbjct: 938 PKGL*EKQSRLAPYPTPSMSQYLL*NPIRS 849
>gb|CO514897.1|CO514897 s13dSG56F1200103_328454 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 510
Score = 69.3 bits (145), Expect(2) = 3e-022
Identities = 25/51 (49%), Positives = 35/51 (68%)
Frame = +3 / +1
Query: 390 YYGRGPIQLTHKYNYRLAGQALNLNLVGDPDLVARDPVVAFKTAIWFWMTP 542
YYGRG I + YNY AG+AL +NL+ P+ + ++ +AF+ AIW WMTP
Sbjct: 70 YYGRGAIPIYWNYNYGAAGEALKVNLLDHPEYIEQNATLAFQAAIWKWMTP 222
Score = 50.6 bits (104), Expect(2) = 3e-022
Identities = 18/43 (41%), Positives = 25/43 (58%)
Frame = +3 / +1
Query: 552 KPSCHAVMTGDWTPSATDRAAGRLPGYGLTSNIINGGLECGKG 680
+PS H G+W P+ D R+PG+G T NI+ G CG+G
Sbjct: 235 QPSAHDAFVGNWKPTKNDTMEKRVPGFGATMNILYGEGVCGQG 363
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,353,995
Number of Sequences: 7669
Number of extensions: 89081
Number of successful extensions: 921
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 273802698
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)