TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.489
(1666 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514896.1|CO514896 s13dSG56F1000091_328452 Glandular tr... 168 1e-042
gb|CO515574.1|CO515574 s13dSG47A0300021_418207 Glandular tr... 131 2e-040
gb|CO515747.1|CO515747 s13dSG77D0300030_419643 Glandular tr... 122 5e-038
gb|CO516729.1|CO516729 s13dSG74F0700061_446786 Glandular tr... 105 8e-032
>gb|CO514896.1|CO514896 s13dSG56F1000091_328452 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 534
Score = 168 bits (362), Expect = 1e-042
Identities = 73/88 (82%), Positives = 79/88 (89%)
Frame = +2 / +3
Query: 1049 ITNGAGLASYPIDTVRRRMMMTSGEAVKYKSSLDAFQQILKKEGPKSLFKGAGANILRAI 1228
IT GAGLASYPI TVRRRMMMTSGEAVKY SSL AFQ I+ KEG KSLFKGAGANILRA+
Sbjct: 6 ITIGAGLASYPIGTVRRRMMMTSGEAVKYNSSLHAFQTIVAKEGTKSLFKGAGANILRAV 185
Query: 1229 AGAGVLSGYDQLQILFFGKKYGSGGA*M 1312
AGAGVL+GYD+LQ++ FGKKYGSGG *M
Sbjct: 186 AGAGVLAGYDKLQLVLFGKKYGSGGG*M 269
Score = 114 bits (244), Expect = 3e-026
Identities = 54/88 (61%), Positives = 59/88 (67%)
Frame = -2 / -3
Query: 1311 I*APPEPYFFPKKRIWSWS*PESTXXXXXXXXXXXXXXLNRDLGPSFLRICWNASKELLY 1132
I* PPEPYFFP K S S*P ST L RDL PSF + WNA KELLY
Sbjct: 268 I*PPPEPYFFPNKTSCSLS*PASTPAPATARNMFAPAPLKRDLVPSFATMVWNACKELLY 89
Query: 1131 LTASPDVIIILLRTVSMG*DARPAPLVI 1048
TASP+VIIILLRTV MG*+A+PAP+VI
Sbjct: 88 FTASPEVIIILLRTVPMG*EAKPAPIVI 5
>gb|CO515574.1|CO515574 s13dSG47A0300021_418207 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 542
Score = 131 bits (280), Expect(2) = 2e-040
Identities = 55/65 (84%), Positives = 61/65 (93%)
Frame = +2 / +2
Query: 401 NFMIDFMMGGVSAAVSKTAAAPIERVKLLIQNQDEMIKSGRLSEPYKGIVDCFKRTIKDE 580
+F+IDF+MGGVSAAVSKTAAAPIER+KLLIQNQDEMIKSGRLSEPYKGI DCFKRT+ DE
Sbjct: 347 HFVIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKSGRLSEPYKGIGDCFKRTMADE 526
Query: 581 GFSSL 595
G +L
Sbjct: 527 GVVAL 541
Score = 50.6 bits (104), Expect(2) = 2e-040
Identities = 22/60 (36%), Positives = 32/60 (53%)
Frame = +2 / +2
Query: 137 QVAMADQANQPTVLHKLGGQFHLRSIISEGVRARNICPSVSSYERRFATRNYMTQSLWGP 316
+ M DQA QPT++ K+ GQ H RS +S G+R + +RF+ NY +L P
Sbjct: 77 KATMVDQARQPTIMEKVTGQLHRRSGLSSGIRGYEGTYRHPAMSQRFSYGNYSNAALQNP 256
>gb|CO515747.1|CO515747 s13dSG77D0300030_419643 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 523
Score = 122 bits (262), Expect(2) = 5e-038
Identities = 51/57 (89%), Positives = 56/57 (98%)
Frame = +2 / +1
Query: 401 NFMIDFMMGGVSAAVSKTAAAPIERVKLLIQNQDEMIKSGRLSEPYKGIVDCFKRTI 571
+F+IDF+MGGVSAAVSKTAAAPIER+KLLIQNQDEMIKSGRLSEPYKGI DCFKRT+
Sbjct: 352 HFVIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKSGRLSEPYKGIGDCFKRTM 522
Score = 50.6 bits (104), Expect(2) = 5e-038
Identities = 22/60 (36%), Positives = 32/60 (53%)
Frame = +2 / +1
Query: 137 QVAMADQANQPTVLHKLGGQFHLRSIISEGVRARNICPSVSSYERRFATRNYMTQSLWGP 316
+ M DQA QPT++ K+ GQ H RS +S G+R + +RF+ NY +L P
Sbjct: 82 KATMVDQARQPTIMEKVTGQLHRRSGLSSGIRGYEGTYRHPAMSQRFSYGNYSNAALQNP 261
>gb|CO516729.1|CO516729 s13dSG74F0700061_446786 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 504
Score = 105 bits (224), Expect(2) = 8e-032
Identities = 44/48 (91%), Positives = 48/48 (100%)
Frame = +2 / +2
Query: 401 NFMIDFMMGGVSAAVSKTAAAPIERVKLLIQNQDEMIKSGRLSEPYKG 544
+F+IDF+MGGVSAAVSKTAAAPIER+KLLIQNQDEMIKSGRLSEPYKG
Sbjct: 359 HFVIDFLMGGVSAAVSKTAAAPIERIKLLIQNQDEMIKSGRLSEPYKG 502
Score = 47.3 bits (97), Expect(2) = 8e-032
Identities = 21/60 (35%), Positives = 30/60 (50%)
Frame = +2 / +2
Query: 137 QVAMADQANQPTVLHKLGGQFHLRSIISEGVRARNICPSVSSYERRFATRNYMTQSLWGP 316
+ M DQ QPT++ K+ GQ H RS +S G R + +RF+ NY +L P
Sbjct: 89 KATMVDQVKQPTIMEKVTGQLHCRSGLSSGFRGYEGTYRHPAMSQRFSYGNYSNAALQNP 268
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,661,145
Number of Sequences: 7669
Number of extensions: 189899
Number of successful extensions: 1215
Number of sequences better than 1.0e-020: 4
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 464677320
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)