TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.458
(708 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514638.1|CO514638 s13dSG46C0400034_327936 Glandular tr... 242 5e-065
gb|CO513887.1|CO513887 s13dSG73G0300020_156436 Glandular tr... 237 2e-063
>gb|CO514638.1|CO514638 s13dSG46C0400034_327936 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 567
Score = 242 bits (522), Expect = 5e-065
Identities = 95/118 (80%), Positives = 108/118 (91%)
Frame = +1 / +2
Query: 82 SCSVEMVKGRTGQRVRLYVRGTVLGYKRSKSNQYETTSLVQIEGVNTKEDVAWYCGKRMA 261
+ +V+MVKGR G+RVRLYVRGT+LGYKRSK NQY TSLVQIEGVNTKE+VAWY GK+MA
Sbjct: 11 AATVKMVKGRQGERVRLYVRGTILGYKRSKVNQYPNTSLVQIEGVNTKEEVAWYAGKKMA 190
Query: 262 YIYKAKTKSSGTHYRCIWGKVARPHGNSGVVRAKFRSNLPPESMGRKVRVFMYPSSI* 435
YIYKAK K +GTHYRCIWGKV RPHGNSG+VRAKF+SNLPP+SMG +VRVFMYPS+I*
Sbjct: 191 YIYKAKVKKNGTHYRCIWGKVTRPHGNSGIVRAKFKSNLPPKSMGSRVRVFMYPSNI* 364
Score = 168 bits (361), Expect = 8e-043
Identities = 78/115 (67%), Positives = 82/115 (71%)
Frame = -2 / -1
Query: 431 MLLGYMNTLTLRPMDSGGRLDLNLARTTPELPCGRATLPQMQR*WVPXXXXXXX*M*AIR 252
MLLGY+NTLT P+D GGR DLNLART P LP G TLP MQR*WVP *+ AI
Sbjct: 360 MLLGYINTLTRDPIDLGGRFDLNLARTIPLLP*GLVTLPHMQR*WVPFFFTLAL*IYAIF 181
Query: 251 FPQYHATSSLVFTPSICTSDVVSYWFDLDRLYPRTVPRT*SLTRCPVRPFTISTL 87
P Y ATSSLVFTPSI T DV YWF LD LYPR VP T*SLTR P RPFTI T+
Sbjct: 180 LPAYQATSSLVFTPSIWTRDVFGYWFTLDLLYPRIVPLT*SLTRSP*RPFTIFTV 16
Score = 103 bits (220), Expect = 2e-023
Identities = 39/81 (48%), Positives = 57/81 (70%)
Frame = +3 / +1
Query: 96 DGEGAHGAAREALRPGHRPWIQAVQVEPVRDYVAGADRGGEHQGGRRVVLREADGLHLQG 275
DGEG A +ALR G+ PWIQ +Q +PV +++G + EHQG +V R+ +G++LQG
Sbjct: 25 DGEGTSRRACQALREGYNPWIQKIQSKPVPKHISGPN*RSEHQGRGSLVCRQENGIYLQG 204
Query: 276 QDQEQRHPLPLHLGQGRPPAW 338
Q +E+R+PLPLH+GQG +W
Sbjct: 205 QGEEERNPLPLHMGQGYKASW 267
>gb|CO513887.1|CO513887 s13dSG73G0300020_156436 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 551
Score = 237 bits (511), Expect = 2e-063
Identities = 92/115 (80%), Positives = 105/115 (91%)
Frame = +1 / +2
Query: 91 VEMVKGRTGQRVRLYVRGTVLGYKRSKSNQYETTSLVQIEGVNTKEDVAWYCGKRMAYIY 270
++MVKGR G+R+RLYVRGTVLGYKRSK NQY TSLVQIEGVNTKE+VAWY GK++AYIY
Sbjct: 65 IKMVKGRQGERIRLYVRGTVLGYKRSKVNQYPNTSLVQIEGVNTKEEVAWYAGKKLAYIY 244
Query: 271 KAKTKSSGTHYRCIWGKVARPHGNSGVVRAKFRSNLPPESMGRKVRVFMYPSSI* 435
KAK K G+HYRCIWGKV RPHGNSG+VRAKF+SNLPP+SMG +VRVFMYPS+I*
Sbjct: 245 KAKVKKDGSHYRCIWGKVTRPHGNSGIVRAKFKSNLPPKSMGARVRVFMYPSNI* 409
Score = 163 bits (350), Expect = 3e-041
Identities = 76/112 (67%), Positives = 81/112 (72%)
Frame = -2 / -3
Query: 431 MLLGYMNTLTLRPMDSGGRLDLNLARTTPELPCGRATLPQMQR*WVPXXXXXXX*M*AIR 252
+LLGY+NTLTL P+D GGRLDLN ART P LP G TLPQMQR*W+P *M A
Sbjct: 405 ILLGYINTLTLAPIDFGGRLDLNFARTMPLLP*GLVTLPQMQR*WLPSFFTLAL*MYANF 226
Query: 251 FPQYHATSSLVFTPSICTSDVVSYWFDLDRLYPRTVPRT*SLTRCPVRPFTI 96
FP YHATSSLVFTPSI T +V Y F LD LYPRTVP T*SL R P RP TI
Sbjct: 225 FPAYHATSSLVFTPSI*TREVFGY*FTLDLLYPRTVPLT*SLIRSPCRPLTI 70
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,157,064
Number of Sequences: 7669
Number of extensions: 58199
Number of successful extensions: 675
Number of sequences better than 1.0e-020: 2
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 178808710
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)