TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.452
(1348 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
emb|AX776636.1| Sequence 5 from Patent WO02102841 71 3e-035
emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulatio... 71 3e-035
emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulatio... 62 1e-031
emb|AX776635.1| Sequence 4 from Patent WO02102841 62 1e-031
gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated... 66 1e-031
gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular tr... 90 1e-028
gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular tr... 70 1e-022
gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular tr... 45 1e-019
gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular tr... 51 3e-015
gb|L07042.1|ALFERKMAP Medicago sativa MAP kinase MsERK1 mRN... 52 1e-007
emb|X66469.1|MSMSK7 M.sativa mRNA msk7 for map kinase 52 1e-007
gb|CO514154.1|CO514154 s13dSG75H0800064_156970 Glandular tr... 52 2e-007
emb|X82268.1|MSMMK2 M.sativa MMK2 mRNA for protein kinase 52 2e-007
gb|AF458700.1| Medicago sativa putative ankyrin-kinase (apk... 43 2e-007
emb|AJ224336.1|MSAJ4336 Medicago sativa mRNA for MAP kinase... 51 2e-007
emb|AJ575100.1|MSA575100 Medicago sativa mRNA for putative ... 37 3e-007
gb|CO515446.1|CO515446 s13dSG50H0600060_417951 Glandular tr... 44 5e-005
gb|AY327889.1| Medicago sativa putative serine/threonine-sp... 44 5e-005
gb|CO516163.1|CO516163 s13dSG67B0600057_445654 Glandular tr... 32 1e-004
gb|CO514405.1|CO514405 s13dSG36B1000079_327470 Glandular tr... 27 3e-004
emb|AX468574.1| Sequence 15 from Patent WO0238745 41 4e-004
emb|AJ293274.1|MVA293274 Medicago sativa subsp. x varia mRN... 41 4e-004
gb|AW698373.1|AW698373 G219 glandular-haired subtracted cDN... 41 4e-004
emb|AJ293275.1|MVA293275 Medicago sativa subsp. x varia mRN... 40 5e-004
gb|CO514123.1|CO514123 s13dSG75E0800055_156908 Glandular tr... 39 0.001
emb|X97314.1|MSCDC2MSC M.sativa mRNA for cdc2 kinase homolo... 36 0.008
gb|CO514053.1|CO514053 s13dSG72G0600040_156768 Glandular tr... 25 0.012
gb|CO513167.1|CO513167 s13dSG23F0700059_129498 Glandular tr... 35 0.016
emb|X82270.1|MSMMK4 M.sativa MMK4 mRNA for protein kinase 35 0.016
gb|CO513822.1|CO513822 s13dSG73A0800053_156306 Glandular tr... 28 0.051
gb|CO515005.1|CO515005 s13dSG26B1000089_397515 Glandular tr... 27 0.053
gb|AF129087.1|AF129087 Medicago sativa mitogen-activated pr... 32 0.20
gb|Y16130.1|Y16130 Y16130 young root nodules Medicago sativ... 27 0.30
gb|AF302013.1| Medicago sativa subsp. x varia CDK-activatin... 31 0.38
gb|CO516997.1|CO516997 s13dSG81F0900077_468540 Glandular tr... 28 0.84
gb|CO514857.1|CO514857 s13dSG56B1100093_328374 Glandular tr... 28 2.6
gb|CO515330.1|CO515330 s13dSG48F1200103_417719 Glandular tr... 28 2.6
gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA... 28 2.6
emb|AJ409164.1|MSA409164 Medicago sativa mRNA for ZIK1 protein 28 2.6
gb|AJ410144.1|AJ410144 AJ410144 Medicago sativa library (Ka... 28 3.5
gb|CO511818.1|CO511818 s13dSG02D0400042_103536 Glandular tr... 27 4.8
gb|CO511946.1|CO511946 s13dSG05H0600060_103792 Glandular tr... 27 4.8
gb|CO514341.1|CO514341 s13dSG84D0400030_157344 Glandular tr... 27 4.8
gb|CO515361.1|CO515361 s13dSG49C0400034_417781 Glandular tr... 25 5.3
gb|CO512859.1|CO512859 s13dSG20E1000071_121644 Glandular tr... 24 6.1
gb|CO513901.1|CO513901 s13dSG73H0500044_156464 Glandular tr... 27 6.6
gb|CO514107.1|CO514107 s13dSG75D0400030_156876 Glandular tr... 27 6.6
gb|L01660.1|ALFNADHSYN Medicago sativa NADH-glutamate synth... 27 6.6
emb|AJ401228.1|MSA401228 Medicago sativa mRNA for selenium ... 27 6.6
emb|X97316.1|MSCDC2MSE M.sativa mRNA for cdc2 kinase homolo... 27 6.6
gb|DQ122809.1| Medicago sativa clone P24 nuclear pore compl... 24 7.7
emb|X59930.1|MFSTRESS M.falcata mRNA for environmental stre... 24 8.2
gb|AW698364.1|AW698364 G210 glandular-haired subtracted cDN... 26 9.1
gb|CO512338.1|CO512338 s13dSG68G0800056_114190 Glandular tr... 26 9.1
gb|CO515693.1|CO515693 s13dSG60F0900079_419535 Glandular tr... 26 9.1
gb|CO515884.1|CO515884 s13dSG91C1100084_421307 Glandular tr... 26 9.1
gb|CO516320.1|CO516320 s13dSG99A0700051_445968 Glandular tr... 26 9.1
gb|AF505625.1| Medicago sativa putative basic helix-loop-he... 26 9.1
>emb|AX776636.1| Sequence 5 from Patent WO02102841
Length = 3339
Score = 70.7 bits (148), Expect(4) = 3e-035
Identities = 30/68 (44%), Positives = 42/68 (61%)
Frame = +2 / +3
Query: 422 LEDILHSAKKPCTLSLPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPK 601
L+ + A K L P RL IA+G+A +AY+H+ + +H D+K SNILLD + K
Sbjct: 2277 LDRLYGEASKRKILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAK 2456
Query: 602 VSDFGSSK 625
V+DFG SK
Sbjct: 2457 VADFGFSK 2480
Score = 51.5 bits (106), Expect(4) = 3e-035
Identities = 21/44 (47%), Positives = 29/44 (65%)
Frame = +2 / +3
Query: 641 SYYVRAVAADIGYMDPLYMKTEHFTLECDVYSFGVVLLELITRR 772
SY V GY+DP Y KT+ + + DV+SFGVVLLE+++ R
Sbjct: 2502 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGR 2633
Score = 46.0 bits (94), Expect(4) = 3e-035
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = +2 / +3
Query: 335 RHKNLVQLLGCCLETDIPILVFEFVANGSLEDILH 439
+H+NLV LLG C E D ILV+ F++NGSL D L+
Sbjct: 2187 QHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLY 2291
Score = 44.6 bits (91), Expect(4) = 3e-035
Identities = 17/47 (36%), Positives = 29/47 (61%)
Frame = +2 / +3
Query: 53 LNAQGITTYTKRELKKITNGYSKRLGGGHFGNVYEGTIVDGRKVAVK 193
+ + + +T +++ T Y +G G FG+VY GT+ DG++VAVK
Sbjct: 1977 IKSVSVKPFTLEYIEQATEQYKTLIGEGGFGSVYRGTLDDGQEVAVK 2117
Score = 30.4 bits (60), Expect(3) = 0.002
Identities = 16/31 (51%), Positives = 19/31 (61%)
Frame = -2 / -3
Query: 435 SMSSRLPLATNSKTRIGMSVSRQHPRSWTKF 343
S+SS+ PL N TRI S S Q PR T+F
Sbjct: 2287 SLSSKEPLDMNGYTRIC*SYSLQ*PRRGTRF 2195
Score = 26.7 bits (52), Expect(3) = 0.002
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -2 / -3
Query: 756 SRSTTPKL*TSHSNVKCSVFIYNGSM*P 673
S TTPKL TS + C V Y+GS P
Sbjct: 2617 SSRTTPKLKTSLFSDNCWVL*YSGSRYP 2534
Score = 24.4 bits (47), Expect(3) = 0.002
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -1 / -2
Query: 1018 LKLLDNLCHGWPL 980
L+L DN+ H WP+
Sbjct: 2852 LQLADNINHAWPI 2814
>emb|AJ418368.1|MSA418368 Medicago sativa mRNA for nodulation receptor kinase (nork gene), wild
type allele DMI2
Length = 3339
Score = 70.7 bits (148), Expect(4) = 3e-035
Identities = 30/68 (44%), Positives = 42/68 (61%)
Frame = +2 / +3
Query: 422 LEDILHSAKKPCTLSLPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPK 601
L+ + A K L P RL IA+G+A +AY+H+ + +H D+K SNILLD + K
Sbjct: 2277 LDRLYGEASKRKILDWPTRLSIALGAARGLAYLHTFPGRSVIHRDVKSSNILLDQSMCAK 2456
Query: 602 VSDFGSSK 625
V+DFG SK
Sbjct: 2457 VADFGFSK 2480
Score = 51.5 bits (106), Expect(4) = 3e-035
Identities = 21/44 (47%), Positives = 29/44 (65%)
Frame = +2 / +3
Query: 641 SYYVRAVAADIGYMDPLYMKTEHFTLECDVYSFGVVLLELITRR 772
SY V GY+DP Y KT+ + + DV+SFGVVLLE+++ R
Sbjct: 2502 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGR 2633
Score = 46.0 bits (94), Expect(4) = 3e-035
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = +2 / +3
Query: 335 RHKNLVQLLGCCLETDIPILVFEFVANGSLEDILH 439
+H+NLV LLG C E D ILV+ F++NGSL D L+
Sbjct: 2187 QHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLY 2291
Score = 44.6 bits (91), Expect(4) = 3e-035
Identities = 17/47 (36%), Positives = 29/47 (61%)
Frame = +2 / +3
Query: 53 LNAQGITTYTKRELKKITNGYSKRLGGGHFGNVYEGTIVDGRKVAVK 193
+ + + +T +++ T Y +G G FG+VY GT+ DG++VAVK
Sbjct: 1977 IKSVSVKPFTLEYIEQATEQYKTLIGEGGFGSVYRGTLDDGQEVAVK 2117
Score = 30.4 bits (60), Expect(3) = 0.002
Identities = 16/31 (51%), Positives = 19/31 (61%)
Frame = -2 / -3
Query: 435 SMSSRLPLATNSKTRIGMSVSRQHPRSWTKF 343
S+SS+ PL N TRI S S Q PR T+F
Sbjct: 2287 SLSSKEPLDMNGYTRIC*SYSLQ*PRRGTRF 2195
Score = 26.7 bits (52), Expect(3) = 0.002
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -2 / -3
Query: 756 SRSTTPKL*TSHSNVKCSVFIYNGSM*P 673
S TTPKL TS + C V Y+GS P
Sbjct: 2617 SSRTTPKLKTSLFSDNCWVL*YSGSRYP 2534
Score = 24.4 bits (47), Expect(3) = 0.002
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -1 / -2
Query: 1018 LKLLDNLCHGWPL 980
L+L DN+ H WP+
Sbjct: 2852 LQLADNINHAWPI 2814
>emb|AJ418377.1|MSA418377 Medicago sativa mRNA for nodulation receptor kinase (nork gene),
mutant allele nn1
Length = 3337
Score = 62.0 bits (129), Expect(4) = 1e-031
Identities = 28/68 (41%), Positives = 39/68 (57%)
Frame = +2 / +1
Query: 422 LEDILHSAKKPCTLSLPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPK 601
L+ + A K L P RL IA+G+ +A +H+ +H D+K SNILLD + K
Sbjct: 2275 LDRLYGEASKRKILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAK 2454
Query: 602 VSDFGSSK 625
V+DFG SK
Sbjct: 2455 VADFGFSK 2478
Score = 51.5 bits (106), Expect(4) = 1e-031
Identities = 21/44 (47%), Positives = 29/44 (65%)
Frame = +2 / +1
Query: 641 SYYVRAVAADIGYMDPLYMKTEHFTLECDVYSFGVVLLELITRR 772
SY V GY+DP Y KT+ + + DV+SFGVVLLE+++ R
Sbjct: 2500 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGR 2631
Score = 46.0 bits (94), Expect(4) = 1e-031
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = +2 / +1
Query: 335 RHKNLVQLLGCCLETDIPILVFEFVANGSLEDILH 439
+H+NLV LLG C E D ILV+ F++NGSL D L+
Sbjct: 2185 QHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLY 2289
Score = 40.5 bits (82), Expect(4) = 1e-031
Identities = 16/47 (34%), Positives = 28/47 (59%)
Frame = +2 / +1
Query: 53 LNAQGITTYTKRELKKITNGYSKRLGGGHFGNVYEGTIVDGRKVAVK 193
+ + + +T +++ T Y + G FG+VY GT+ DG++VAVK
Sbjct: 1975 IKSVSVKPFTLEYIEQATEQYKTLICEGGFGSVYRGTLDDGQEVAVK 2115
Score = 29.0 bits (57), Expect(3) = 0.004
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = -2 / -3
Query: 435 SMSSRLPLATNSKTRIGMSVSRQHPRSWTKF 343
++SS+ PL N TRI S S Q PR T+F
Sbjct: 2285 NLSSKEPLDMNGYTRIC*SYSLQ*PRRGTRF 2193
Score = 26.7 bits (52), Expect(3) = 0.004
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -2 / -3
Query: 756 SRSTTPKL*TSHSNVKCSVFIYNGSM*P 673
S TTPKL TS + C V Y+GS P
Sbjct: 2615 SSRTTPKLKTSLFSDNCWVL*YSGSRYP 2532
Score = 24.4 bits (47), Expect(3) = 0.004
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -1 / -2
Query: 1018 LKLLDNLCHGWPL 980
L+L DN+ H WP+
Sbjct: 2850 LQLADNINHAWPI 2812
>emb|AX776635.1| Sequence 4 from Patent WO02102841
Length = 3378
Score = 62.0 bits (129), Expect(4) = 1e-031
Identities = 28/68 (41%), Positives = 39/68 (57%)
Frame = +2 / +1
Query: 422 LEDILHSAKKPCTLSLPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPK 601
L+ + A K L P RL IA+G+ +A +H+ +H D+K SNILLD + K
Sbjct: 2296 LDRLYGEASKRKILDWPTRLSIALGAPRGLA*LHTFPGPSVIHRDLKSSNILLDQSMCAK 2475
Query: 602 VSDFGSSK 625
V+DFG SK
Sbjct: 2476 VADFGFSK 2499
Score = 51.5 bits (106), Expect(4) = 1e-031
Identities = 21/44 (47%), Positives = 29/44 (65%)
Frame = +2 / +1
Query: 641 SYYVRAVAADIGYMDPLYMKTEHFTLECDVYSFGVVLLELITRR 772
SY V GY+DP Y KT+ + + DV+SFGVVLLE+++ R
Sbjct: 2521 SYVSLEVRGTAGYLDPEYYKTQQLSEKSDVFSFGVVLLEIVSGR 2652
Score = 46.0 bits (94), Expect(4) = 1e-031
Identities = 20/35 (57%), Positives = 26/35 (74%)
Frame = +2 / +1
Query: 335 RHKNLVQLLGCCLETDIPILVFEFVANGSLEDILH 439
+H+NLV LLG C E D ILV+ F++NGSL D L+
Sbjct: 2206 QHENLVPLLGYCNEYDQQILVYPFMSNGSLLDRLY 2310
Score = 40.5 bits (82), Expect(4) = 1e-031
Identities = 16/47 (34%), Positives = 28/47 (59%)
Frame = +2 / +1
Query: 53 LNAQGITTYTKRELKKITNGYSKRLGGGHFGNVYEGTIVDGRKVAVK 193
+ + + +T +++ T Y + G FG+VY GT+ DG++VAVK
Sbjct: 1996 IKSVSVKPFTLEYIEQATEQYKTLICEGGFGSVYRGTLDDGQEVAVK 2136
Score = 29.0 bits (57), Expect(3) = 0.004
Identities = 15/31 (48%), Positives = 19/31 (61%)
Frame = -2 / -2
Query: 435 SMSSRLPLATNSKTRIGMSVSRQHPRSWTKF 343
++SS+ PL N TRI S S Q PR T+F
Sbjct: 2306 NLSSKEPLDMNGYTRIC*SYSLQ*PRRGTRF 2214
Score = 26.7 bits (52), Expect(3) = 0.004
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -2 / -2
Query: 756 SRSTTPKL*TSHSNVKCSVFIYNGSM*P 673
S TTPKL TS + C V Y+GS P
Sbjct: 2636 SSRTTPKLKTSLFSDNCWVL*YSGSRYP 2553
Score = 24.4 bits (47), Expect(3) = 0.004
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = -1 / -1
Query: 1018 LKLLDNLCHGWPL 980
L+L DN+ H WP+
Sbjct: 2871 LQLADNINHAWPI 2833
>gb|AY563141.1| Medicago sativa calcium/calmodulin-regulated receptor-like kinase
mRNA, complete cds
Length = 1798
Score = 66.1 bits (138), Expect(4) = 1e-031
Identities = 28/56 (50%), Positives = 38/56 (67%)
Frame = +2 / +1
Query: 461 LSLPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPKVSDFGSSKL 628
L + ERLDIAI A AI Y+H + +H DIK SNIL+ ++L KV+DFG ++L
Sbjct: 844 LEIGERLDIAIDIAHAITYLHMYTDNPIIHRDIKASNILISENLRAKVADFGFARL 1011
Score = 50.6 bits (104), Expect(4) = 1e-031
Identities = 20/38 (52%), Positives = 27/38 (71%)
Frame = +2 / +1
Query: 659 VAADIGYMDPLYMKTEHFTLECDVYSFGVVLLELITRR 772
V GYMDP Y++T T + DVYSFGV+L+E++T R
Sbjct: 1048 VKGTAGYMDPEYLRTYQLTEKSDVYSFGVLLVEMMTGR 1161
Score = 39.1 bits (79), Expect(4) = 1e-031
Identities = 18/42 (42%), Positives = 24/42 (57%)
Frame = +2 / +1
Query: 122 RLGGGHFGNVYEGTIVDGRKVAVKCPLRTRVSSHRCHWKNLI 247
++G G FG VY G + DG VAVK + + SH +KN I
Sbjct: 577 QIGEGGFGTVYRGKLNDGTIVAVKRAKKEALQSHLYEFKNEI 702
Score = 30.9 bits (61), Expect(4) = 1e-031
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +2 / +1
Query: 338 HKNLVQLLGCCLETDIPILVFEFVANGSLEDIL 436
H NLV+L G D +++ E+V NG+L + L
Sbjct: 724 HLNLVRLYGYLEHGDEKLIIVEYVGNGNLTEHL 822
Score = 28.6 bits (56), Expect(2) = 0.024
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = -2 / -3
Query: 627 SLEEPKSETFGLRSSSRRMLEGLMS 553
+L +PKS T LR S RM + LMS
Sbjct: 1010 NLAKPKSATLALRFSLIRMFDALMS 936
Score = 23.5 bits (45), Expect(2) = 0.024
Identities = 15/33 (45%), Positives = 19/33 (57%)
Frame = -2 / -3
Query: 771 LLVMSSRSTTPKL*TSHSNVKCSVFIYNGSM*P 673
L V+ S S TP *TS +V V Y+GS+ P
Sbjct: 1160 LPVIISTSNTPNE*TSLFSVS**VLKYSGSIYP 1062
>gb|CO512543.1|CO512543 s13dSG08D0400030_121012 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 611
Score = 90.4 bits (191), Expect(2) = 1e-028
Identities = 43/95 (45%), Positives = 57/95 (60%)
Frame = +2 / +1
Query: 338 HKNLVQLLGCCLETDIPILVFEFVANGSLEDILHSAKKPCTLSLPERLDIAIGSAEAIAY 517
HKNLV+LLG + + ILV+E++ NGSL D LH + +S R+ +A+ +A I Y
Sbjct: 160 HKNLVKLLGFYEDKNGRILVYEYMNNGSLNDHLHKFQTSTIMSWNGRIKVALDAARGIEY 339
Query: 518 MHSLDNQKRVHGDIKPSNILLDDDLNPKVSDFGSS 622
+H +H DIK SNILLD KVSDFG S
Sbjct: 340 LHKYAQPPIIHRDIKTSNILLDSKWVAKVSDFGLS 444
Score = 51.5 bits (106), Expect(2) = 1e-028
Identities = 20/39 (51%), Positives = 28/39 (71%)
Frame = +2 / +1
Query: 662 AADIGYMDPLYMKTEHFTLECDVYSFGVVLLELITRRRA 778
A +GYMDP Y + ++ T + DVYSFGV LLEL++ +A
Sbjct: 490 AGTVGYMDPEYYRLQYLTSKSDVYSFGVXLLELLSGYKA 606
Score = 34.5 bits (69), Expect(3) = 7e-006
Identities = 19/36 (52%), Positives = 23/36 (63%)
Frame = -2 / -1
Query: 780 LALLLVMSSRSTTPKL*TSHSNVKCSVFIYNGSM*P 673
+AL + SS TPKL*TS +VK Y+GSM*P
Sbjct: 608 MALYPLKSSNKXTPKL*TSLFDVKY*SL*YSGSM*P 501
Score = 23.5 bits (45), Expect(3) = 7e-006
Identities = 9/23 (39%), Positives = 13/23 (56%)
Frame = -2 / -1
Query: 621 EEPKSETFGLRSSSRRMLEGLMS 553
+ PKS+T S +M + LMS
Sbjct: 443 DNPKSDTLATHFESNKMFDVLMS 375
Score = 22.6 bits (43), Expect(3) = 7e-006
Identities = 16/35 (45%), Positives = 19/35 (54%)
Frame = -2 / -1
Query: 441 L*SMSSRLPLATNSKTRIGMSVSRQHPRSWTKFL* 337
L* S +LPL S T I +S P + TKFL*
Sbjct: 263 L*RWSFKLPLFMYS*TNILPFLSS*KPSNLTKFL* 159
>gb|CO512977.1|CO512977 s13dSG09B0200013_121880 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 582
Score = 69.8 bits (146), Expect(3) = 1e-022
Identities = 28/59 (47%), Positives = 39/59 (66%)
Frame = +2 / +1
Query: 461 LSLPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPKVSDFGSSKLLAI 637
L+ +RL I G+A+ + Y+H +K VHGD+KPSNILL D+ P +SDFG +L I
Sbjct: 175 LTWSDRLKIMKGTAKGLVYLHEFSPKKYVHGDLKPSNILLGHDMTPYISDFGLGRLANI 351
Score = 40.9 bits (83), Expect = 4e-004
Identities = 22/48 (45%), Positives = 24/48 (50%)
Frame = -2 / -2
Query: 615 PKSETFGLRSSSRRMLEGLMSPWTRF*LSRECM*AIASAEPMAMSSRS 472
PKSE G+ S R ML G SPWT F C * A P + SRS
Sbjct: 329 PKSEM*GVMSCPRSMLLGFRSPWTYFLGLNSCR*TNPFAVPFMIFSRS 186
Score = 37.3 bits (75), Expect(3) = 1e-022
Identities = 16/33 (48%), Positives = 22/33 (66%)
Frame = +2 / +1
Query: 674 GYMDPLYMKTEHFTLECDVYSFGVVLLELITRR 772
GY P +K + + DVYS+GV+LLE+IT R
Sbjct: 457 GYQAPEALKVVKPSQKWDVYSYGVILLEMITGR 555
Score = 32.2 bits (64), Expect(3) = 1e-022
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = +2 / +1
Query: 335 RHKNLVQLLGCCLETDIPILVFEFVANGSLEDILH 439
RH N+ L D +L+++++ NGSL +H
Sbjct: 40 RHPNIATLRAYYWSVDEKLLIYDYIPNGSLASAIH 144
>gb|CO511959.1|CO511959 s13dSG07B0300029_103818 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 596
Score = 44.6 bits (91), Expect(4) = 1e-019
Identities = 19/35 (54%), Positives = 24/35 (68%)
Frame = +2 / +1
Query: 674 GYMDPLYMKTEHFTLECDVYSFGVVLLELITRRRA 778
GY+DP Y + T + D YSFGVVL+ELI+ RA
Sbjct: 445 GYVDPEYYQCYQLTDKSDAYSFGVVLVELISSLRA 549
Score = 40.0 bits (81), Expect(4) = 1e-019
Identities = 15/28 (53%), Positives = 22/28 (78%)
Frame = +2 / +1
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSKL 628
+H D+K +NILLD+ + KV+DFG S+L
Sbjct: 313 MHRDVKSNNILLDEKFHVKVADFGLSRL 396
Score = 35.9 bits (72), Expect(4) = 1e-019
Identities = 15/30 (50%), Positives = 20/30 (66%)
Frame = +2 / +1
Query: 455 CTLSLPERLDIAIGSAEAIAYMHSLDNQKR 544
C L RLDIA+ +AEA+AY+H+ D R
Sbjct: 232 CLLPWSVRLDIALETAEALAYLHASDVMHR 321
Score = 28.6 bits (56), Expect = 1.9
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -2 / -1
Query: 636 IARSLEEPKSETFGLRSSSRRMLEGLMS 553
+ +LE PKS T SSRRML L S
Sbjct: 404 LGNNLERPKSATLTWNFSSRRMLLDLTS 321
Score = 25.4 bits (49), Expect(4) = 1e-019
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +2 / +1
Query: 335 RHKNLVQLLGC 367
RHKNLV L GC
Sbjct: 106 RHKNLVTLYGC 138
>gb|CO511940.1|CO511940 s13dSG05G1200100_103780 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 595
Score = 51.5 bits (106), Expect(2) = 3e-015
Identities = 21/51 (41%), Positives = 33/51 (64%)
Frame = +2 / +1
Query: 476 RLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPKVSDFGSSKL 628
R+ IA G+A + Y+H ++ D+K SNILL +D + K+SDFG +K+
Sbjct: 67 RMKIAAGAARGLEYLHDKMKPPVIYRDLKCSNILLGEDYHSKLSDFGLAKV 219
Score = 45.1 bits (92), Expect(2) = 3e-015
Identities = 20/40 (50%), Positives = 23/40 (57%)
Frame = +2 / +1
Query: 659 VAADIGYMDPLYMKTEHFTLECDVYSFGVVLLELITRRRA 778
V GY P Y T T + D+YS GV LLELIT R+A
Sbjct: 256 VMGTYGYCAPDYAMTGQLTFKSDIYSIGVALLELITGRKA 375
>gb|L07042.1|ALFERKMAP Medicago sativa MAP kinase MsERK1 mRNA, complete cds
Length = 1529
Score = 52.4 bits (108), Expect = 1e-007
Identities = 22/76 (28%), Positives = 42/76 (55%)
Frame = +2 / +3
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSKLLAIHSYYVRAVAADIGYMDPLYMKTEHFTLEC 724
+H D+KPSN+LL+ + + K+ DFG +++ + + V L + + +T
Sbjct: 633 LHRDLKPSNLLLNANCDLKICDFGLARVTSETDFMTEYVVTRWYRAPELLLNSSDYTAAI 812
Query: 725 DVYSFGVVLLELITRR 772
DV+S G + +EL+ R+
Sbjct: 813 DVWSVGCIFMELMDRK 860
>emb|X66469.1|MSMSK7 M.sativa mRNA msk7 for map kinase
Length = 1512
Score = 52.4 bits (108), Expect = 1e-007
Identities = 22/76 (28%), Positives = 42/76 (55%)
Frame = +2 / +1
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSKLLAIHSYYVRAVAADIGYMDPLYMKTEHFTLEC 724
+H D+KPSN+LL+ + + K+ DFG +++ + + V L + + +T
Sbjct: 631 LHRDLKPSNLLLNANCDLKICDFGLARVTSETDFMTEYVVTRWYRAPELLLNSSDYTAAI 810
Query: 725 DVYSFGVVLLELITRR 772
DV+S G + +EL+ R+
Sbjct: 811 DVWSVGCIFMELMDRK 858
>gb|CO514154.1|CO514154 s13dSG75H0800064_156970 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 555
Score = 51.9 bits (107), Expect = 2e-007
Identities = 22/76 (28%), Positives = 41/76 (53%)
Frame = +2 / +2
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSKLLAIHSYYVRAVAADIGYMDPLYMKTEHFTLEC 724
+H D+KPSN+LL+ + + K+ DFG ++ + + V L + +T
Sbjct: 191 LHRDLKPSNLLLNSNCDLKIGDFGLARTTSETDFMTEYVVTRWYRAPELLLNCSEYTAAI 370
Query: 725 DVYSFGVVLLELITRR 772
D++S G +L E++TR+
Sbjct: 371 DIWSVGCILGEVMTRQ 418
>emb|X82268.1|MSMMK2 M.sativa MMK2 mRNA for protein kinase
Length = 1565
Score = 51.9 bits (107), Expect = 2e-007
Identities = 22/76 (28%), Positives = 41/76 (53%)
Frame = +2 / +1
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSKLLAIHSYYVRAVAADIGYMDPLYMKTEHFTLEC 724
+H D+KPSN+LL+ + + K+ DFG ++ + + V L + +T
Sbjct: 760 LHRDLKPSNLLLNANCDLKIGDFGLARTTSETDFMTEYVVTRWYRAPELLLNCSDYTAAI 939
Query: 725 DVYSFGVVLLELITRR 772
D++S G +L E++TR+
Sbjct: 940 DIWSVGCILGEIVTRQ 987
>gb|AF458700.1| Medicago sativa putative ankyrin-kinase (apk1) mRNA, complete cds
Length = 1815
Score = 42.8 bits (87), Expect(2) = 2e-007
Identities = 22/70 (31%), Positives = 35/70 (50%)
Frame = +2 / +1
Query: 428 DILHSAKKPCTLSLPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPKVS 607
D+ +K LS + L ++ A + Y+H +H D+KP NILLD+ KV+
Sbjct: 889 DLTGYIQKKGRLSPSKVLRFSLDIARGMNYLHECKPDPIIHCDLKPKNILLDNGGQLKVA 1068
Query: 608 DFGSSKLLAI 637
FG+ + I
Sbjct: 1069 GFGTVRFSLI 1098
Score = 27.2 bits (53), Expect(2) = 2e-007
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +2 / +1
Query: 677 YMDPLYMKTEHFTLECDVYSFGVVLLELI 763
Y+ P + + F D YSFG+++ E+I
Sbjct: 1156 YVAPEIYRGDVFDRSVDAYSFGLIVYEMI 1242
>emb|AJ224336.1|MSAJ4336 Medicago sativa mRNA for MAP kinase, complete CDS
Length = 1442
Score = 51.5 bits (106), Expect = 2e-007
Identities = 23/75 (30%), Positives = 39/75 (52%)
Frame = +2 / +1
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSKLLAIHSYYVRAVAADIGYMDPLYMKTEHFTLEC 724
+H D+KPSN+LL + + K+ DFG ++ + + V L + +T
Sbjct: 520 LHRDLKPSNLLLKANCDLKICDFGLARTTSETDFMTEYVVTRWYRAPELLLNCSEYTAAI 699
Query: 725 DVYSFGVVLLELITR 769
DV+S G +L+E+I R
Sbjct: 700 DVWSVGCILMEIIRR 744
>emb|AJ575100.1|MSA575100 Medicago sativa mRNA for putative mitogen-activated protein kinase 1
(mekk1 gene)
Length = 1840
Score = 37.3 bits (75), Expect(3) = 3e-007
Identities = 16/38 (42%), Positives = 27/38 (71%)
Frame = +2 / +1
Query: 527 LDNQKRVHGDIKPSNILLDDDLNPKVSDFGSSKLLAIH 640
L ++ VH DIK +NIL+D + + KV+DFG +K + ++
Sbjct: 1327 LHDRNIVHRDIKCANILVDANGSVKVADFGLAKAIKLN 1440
Score = 30.4 bits (60), Expect(3) = 3e-007
Identities = 14/23 (60%), Positives = 14/23 (60%)
Frame = +2 / +1
Query: 125 LGGGHFGNVYEGTIVDGRKVAVK 193
LG G FG VYEG DG AVK
Sbjct: 1003 LGRGSFGTVYEGISEDGFFFAVK 1071
Score = 25.8 bits (50), Expect(3) = 3e-007
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +2 / +1
Query: 338 HKNLVQLLGCCLETDIPILVFEFVANGSL 424
H+N+V+ +G ++ + EFV GSL
Sbjct: 1159 HENIVRYIGTEMDESNLYIFIEFVTKGSL 1245
>gb|CO515446.1|CO515446 s13dSG50H0600060_417951 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 531
Score = 43.7 bits (89), Expect = 5e-005
Identities = 18/40 (45%), Positives = 26/40 (65%)
Frame = +2 / +3
Query: 647 YVRAVAADIGYMDPLYMKTEHFTLECDVYSFGVVLLELIT 766
+V ++GY+ P Y T +L+ DVY FGV+LLEL+T
Sbjct: 180 FVNGDLGELGYIAPEYSSTMVASLKGDVYGFGVLLLELVT 299
>gb|AY327889.1| Medicago sativa putative serine/threonine-specific receptor-like
protein kinase gene, partial cds
Length = 547
Score = 43.7 bits (89), Expect = 5e-005
Identities = 19/48 (39%), Positives = 29/48 (60%)
Frame = +2 / +3
Query: 674 GYMDPLYMKTEHFTLECDVYSFGVVLLELITRRRASWYEQDQQGNKIL 817
GYM P Y F+++ DV+SFGVV+LE+I+ R+ + Q +L
Sbjct: 6 GYMPPEYAVHGSFSIKSDVFSFGVVVLEIISGRKNRGFYDPQHHLNLL 149
>gb|CO516163.1|CO516163 s13dSG67B0600057_445654 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 535
Score = 32.2 bits (64), Expect(3) = 1e-004
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = +2 / +3
Query: 392 LVFEFVANGSLEDILHSAKKPCTLSLPERLDIAIGSAEAIAYMHS 526
LV+E V+NG+ + L + ERL + I A+AI ++H+
Sbjct: 231 LVYE*VSNGNFQSYLSGDSCGKIFNWSERLSVLISVAKAIHFLHT 365
Score = 24.9 bits (48), Expect(3) = 1e-004
Identities = 8/24 (33%), Positives = 14/24 (58%)
Frame = +2 / +3
Query: 125 LGGGHFGNVYEGTIVDGRKVAVKC 196
LG +G +Y+G + G V ++C
Sbjct: 15 LGENIYGKLYKGKLESGIAVVIRC 86
Score = 23.5 bits (45), Expect(3) = 1e-004
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +2 / +3
Query: 335 RHKNLVQLLGCCLE 376
RH +LV LLG C++
Sbjct: 153 RHTHLVSLLGHCID 194
>gb|CO514405.1|CO514405 s13dSG36B1000079_327470 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 550
Score = 27.2 bits (53), Expect(3) = 3e-004
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +2 / +3
Query: 329 VSRHKNLVQLLGCCLETD 382
V +H NLV+L+G C E D
Sbjct: 396 VIKHPNLVRLVGYCAEDD 449
Score = 26.3 bits (51), Expect(3) = 3e-004
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +2 / +2
Query: 125 LGGGHFGNVYEGTIV 169
+G G FG+VY GT+V
Sbjct: 239 IGEGGFGSVYRGTLV 283
Score = 25.8 bits (50), Expect(3) = 3e-004
Identities = 10/16 (62%), Positives = 14/16 (87%)
Frame = +2 / +3
Query: 389 ILVFEFVANGSLEDIL 436
+LV+EF++N SLED L
Sbjct: 468 LLVYEFMSNKSLEDHL 515
>emb|AX468574.1| Sequence 15 from Patent WO0238745
Length = 1495
Score = 40.9 bits (83), Expect = 4e-004
Identities = 17/55 (30%), Positives = 32/55 (58%)
Frame = +2 / +1
Query: 467 LPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPKVSDFGSSKLL 631
+P+ +A + + + + L + VH DIKPSN+L++ K++DFG ++L
Sbjct: 664 IPQENQLADVARQILRGLAYLHRRHIVHRDIKPSNLLINSRKQVKIADFGVGRIL 828
>emb|AJ293274.1|MVA293274 Medicago sativa subsp. x varia mRNA for MEK map kinase kinase
(simkk gene)
Length = 1536
Score = 40.9 bits (83), Expect = 4e-004
Identities = 17/55 (30%), Positives = 32/55 (58%)
Frame = +2 / +2
Query: 467 LPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKPSNILLDDDLNPKVSDFGSSKLL 631
+P+ +A + + + + L + VH DIKPSN+L++ K++DFG ++L
Sbjct: 680 IPQENQLADVARQILRGLAYLHRRHIVHRDIKPSNLLINSRKQVKIADFGVGRIL 844
>gb|AW698373.1|AW698373 G219 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 184
Score = 40.9 bits (83), Expect = 4e-004
Identities = 17/27 (62%), Positives = 20/27 (74%)
Frame = +2 / -2
Query: 548 HGDIKPSNILLDDDLNPKVSDFGSSKL 628
H D+KP N+LLD + N KVSDFG S L
Sbjct: 123 HRDLKPQNLLLDAEGNLKVSDFGLSAL 43
Score = 29.5 bits (58), Expect = 0.99
Identities = 14/23 (60%), Positives = 15/23 (65%)
Frame = -2 / +2
Query: 621 EEPKSETFGLRSSSRRMLEGLMS 553
E PKSET L S+SRR GL S
Sbjct: 50 ERPKSETLRLPSASRRRFCGLRS 118
>emb|AJ293275.1|MVA293275 Medicago sativa subsp. x varia mRNA for MAP kinase kinase (PRK
gene)
Length = 1471
Score = 40.5 bits (82), Expect = 5e-004
Identities = 16/48 (33%), Positives = 25/48 (52%)
Frame = +2 / +1
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSKLLAIHSYYVRAVAADIGYMDP 688
+H D+KPSN+L++ K++DFG S ++ S YM P
Sbjct: 568 IHRDLKPSNLLINHTGEVKITDFGVSAIMESTSGQANTFIGTYNYMSP 711
>gb|CO514123.1|CO514123 s13dSG75E0800055_156908 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 244
Score = 39.1 bits (79), Expect = 0.001
Identities = 15/27 (55%), Positives = 20/27 (74%)
Frame = +2 / +2
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSK 625
++ D K SNILLD + N K+SDFG +K
Sbjct: 47 IYRDFKASNILLDGNYNAKISDFGLAK 127
>emb|X97314.1|MSCDC2MSC M.sativa mRNA for cdc2 kinase homologue, cdc2MsC
Length = 1789
Score = 36.3 bits (73), Expect = 0.008
Identities = 14/36 (38%), Positives = 25/36 (69%)
Frame = +2 / +3
Query: 518 MHSLDNQKRVHGDIKPSNILLDDDLNPKVSDFGSSK 625
+H + +H DIK SN+L+D++ N K++DFG ++
Sbjct: 540 LHYCHVNQVLHRDIKGSNLLIDNEGNLKLADFGLAR 647
>gb|CO514053.1|CO514053 s13dSG72G0600040_156768 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 552
Score = 25.4 bits (49), Expect(3) = 0.012
Identities = 11/22 (50%), Positives = 15/22 (68%)
Frame = +2 / +1
Query: 389 ILVFEFVANGSLEDILHSAKKP 454
+LV+EF+ N SLE+ L S P
Sbjct: 469 LLVYEFMPNRSLENHLFSRTLP 534
Score = 24.9 bits (48), Expect(3) = 0.012
Identities = 8/15 (53%), Positives = 13/15 (86%)
Frame = +2 / +1
Query: 122 RLGGGHFGNVYEGTI 166
++G G FG+VY+G+I
Sbjct: 244 KIGEGGFGSVYKGSI 288
Score = 23.1 bits (44), Expect(3) = 0.012
Identities = 8/14 (57%), Positives = 10/14 (71%)
Frame = +2 / +1
Query: 329 VSRHKNLVQLLGCC 370
+ H NLV+LLG C
Sbjct: 397 IVNHPNLVKLLGYC 438
>gb|CO513167.1|CO513167 s13dSG23F0700059_129498 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 592
Score = 35.4 bits (71), Expect = 0.016
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +2 / +2
Query: 527 LDNQKRVHGDIKPSNILLDDDLNPKVSDFGSSK 625
L + VH DIK +NIL+D + K++DFG +K
Sbjct: 254 LHGRNTVHRDIKGANILVDPNGEIKLADFGMAK 352
>emb|X82270.1|MSMMK4 M.sativa MMK4 mRNA for protein kinase
Length = 1695
Score = 35.4 bits (71), Expect = 0.016
Identities = 12/27 (44%), Positives = 21/27 (77%)
Frame = +2 / +1
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSK 625
+H D+KPSN+LL+ + + K+ DFG ++
Sbjct: 658 IHRDLKPSNLLLNANCDLKIIDFGLAR 738
>gb|CO513822.1|CO513822 s13dSG73A0800053_156306 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 562
Score = 27.6 bits (54), Expect(3) = 0.051
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = -3 / +2
Query: 743 RRSCRHRTPM*SARSSYIMGPCSLYL 666
R CR R P+ +SY+ G C L L
Sbjct: 284 RMFCRRRNPLGLLSTSYLTGACFLLL 361
Score = 21.7 bits (41), Expect(3) = 0.051
Identities = 8/14 (57%), Positives = 8/14 (57%)
Frame = -3 / +3
Query: 89 PFWCRWLCPGHSVV 48
PFW R C G S V
Sbjct: 441 PFWYRQNCSGWSCV 482
Score = 21.7 bits (41), Expect(3) = 0.051
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = -1 / +2
Query: 1027 LDSLKLLDNLCHGWPLPLVF 968
L S + + + WPLPL+F
Sbjct: 44 LHSAAVKNRISLWWPLPLLF 103
>gb|CO515005.1|CO515005 s13dSG26B1000089_397515 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 455
Score = 27.2 bits (53), Expect(2) = 0.053
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = +2 / +3
Query: 329 VSRHKNLVQLLGCCLETD 382
V +H NLV+L+G C E D
Sbjct: 396 VIKHPNLVRLVGYCAEDD 449
Score = 26.3 bits (51), Expect(2) = 0.053
Identities = 9/15 (60%), Positives = 12/15 (80%)
Frame = +2 / +2
Query: 125 LGGGHFGNVYEGTIV 169
+G G FG+VY GT+V
Sbjct: 239 IGEGGFGSVYRGTLV 283
>gb|AF129087.1|AF129087 Medicago sativa mitogen-activated protein kinase homologue (TDY1)
gene, complete cds
Length = 7346
Score = 31.8 bits (63), Expect = 0.20
Identities = 12/27 (44%), Positives = 18/27 (66%)
Frame = +2 / +3
Query: 548 HGDIKPSNILLDDDLNPKVSDFGSSKL 628
H D+KP NIL + + KV DFG +++
Sbjct: 3591 HRDLKPKNILANANCKLKVCDFGLARV 3671
>gb|Y16130.1|Y16130 Y16130 young root nodules Medicago sativa subsp. x varia cDNA clone
Mscp16 similar to protein kinase homologue, mRNA
sequence
Length = 438
Score = 27.2 bits (53), Expect(2) = 0.30
Identities = 10/29 (34%), Positives = 17/29 (58%)
Frame = +2 / +3
Query: 677 YMDPLYMKTEHFTLECDVYSFGVVLLELI 763
Y+ P + + F D YSFG+++ E+I
Sbjct: 282 YVAPEIYRGDVFDRSVDAYSFGLIVYEMI 368
Score = 23.5 bits (45), Expect(2) = 0.30
Identities = 11/40 (27%), Positives = 19/40 (47%)
Frame = +2 / +2
Query: 446 KKPCTLSLPERLDIAIGSAEAIAYMHSLDNQKRVHGDIKP 565
+K LS + L ++ A + Y+H +H D+KP
Sbjct: 26 QKKGRLSPSKVLRFSLDIARGMNYLHECKPDPIIHCDLKP 145
>gb|AF302013.1| Medicago sativa subsp. x varia CDK-activating kinase (cak) mRNA,
complete cds
Length = 1590
Score = 30.9 bits (61), Expect = 0.38
Identities = 10/28 (35%), Positives = 21/28 (75%)
Frame = +2 / +2
Query: 545 VHGDIKPSNILLDDDLNPKVSDFGSSKL 628
+H D+KP+N+L+ + K++DFG +++
Sbjct: 518 LHRDMKPNNLLIGPNGQLKLADFGLARI 601
>gb|CO516997.1|CO516997 s13dSG81F0900077_468540 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 512
Score = 27.6 bits (54), Expect(2) = 0.84
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = -3 / +2
Query: 743 RRSCRHRTPM*SARSSYIMGPCSLYL 666
R CR R P+ +SY+ G C L L
Sbjct: 386 RMFCRRRNPLGLLSTSYLTGACFLLL 463
Score = 21.7 bits (41), Expect(2) = 0.84
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = -1 / +2
Query: 1027 LDSLKLLDNLCHGWPLPLVF 968
L S + + + WPLPL+F
Sbjct: 146 LHSAAVKNRISLWWPLPLLF 205
>gb|CO514857.1|CO514857 s13dSG56B1100093_328374 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 461
Score = 28.1 bits (55), Expect = 2.6
Identities = 12/34 (35%), Positives = 16/34 (47%)
Frame = +2 / +1
Query: 761 ITRRRASWYEQDQQGNKILPIEFVKCFKDHGSGC 862
+ R + W + N ILP +CFK H S C
Sbjct: 142 VLRLVSFWNVTLVKANVILPAA*CRCFKAHSSAC 243
>gb|CO515330.1|CO515330 s13dSG48F1200103_417719 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 558
Score = 28.1 bits (55), Expect = 2.6
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +2 / +3
Query: 545 VHGDIKPSNILLDDDLNPKVSDFG 616
VH DIK NIL+ +D +++DFG
Sbjct: 480 VHCDIKLQNILVFNDGXYQIADFG 551
>gb|U20431.1|MSU20431 Medicago sativa polygalacturonase mRNA, complete cds
Length = 1492
Score = 28.1 bits (55), Expect = 2.6
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = +3 / +3
Query: 1029 YYCWVHIYKIMACSSQASSKHADEWKRSK 1115
+YC++ I+ + CSS S H W+++K
Sbjct: 48 HYCFIPIHC*LLCSSIWGS*HIKIWRKTK 134
>emb|AJ409164.1|MSA409164 Medicago sativa mRNA for ZIK1 protein
Length = 1776
Score = 28.1 bits (55), Expect = 2.6
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = +2 / +1
Query: 704 EHFTLECDVYSFGVVLLELIT 766
E + D+Y+FG+ LLEL+T
Sbjct: 607 EEYNELVDIYAFGMCLLELVT 669
>gb|AJ410144.1|AJ410144 AJ410144 Medicago sativa library (Kalo P) Medicago sativa cDNA
clone U63, mRNA sequence
Length = 849
Score = 27.6 bits (54), Expect = 3.5
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +2 / +3
Query: 416 GSLEDILHSAKKPCTLSLPERLDIAIG 496
G ++H+ K PC L LP L A+G
Sbjct: 438 GLTPSLVHATKHPCYLGLPIILIXAVG 518
>gb|CO511818.1|CO511818 s13dSG02D0400042_103536 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 493
Score = 27.2 bits (53), Expect = 4.8
Identities = 17/50 (34%), Positives = 23/50 (46%)
Frame = -1 / +1
Query: 364 SQELDQVLVTTDFELEPDLVHERPIFLHPLLR*RYTARSNQILPMAAVRR 215
+ +LD VL TT DLV + L P L + NQ+L AV +
Sbjct: 301 NDDLDSVLQTTPLSRWNDLVFFQNGMLEPWLESKGLKDGNQVLAYFAVSK 450
>gb|CO511946.1|CO511946 s13dSG05H0600060_103792 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 370
Score = 27.2 bits (53), Expect = 4.8
Identities = 11/31 (35%), Positives = 14/31 (45%)
Frame = -3 / +3
Query: 506 LPRSQWRCPAAPAARGYMVSWHYEVCPPGFH 414
L R W C + R +V W + V PP H
Sbjct: 225 LTRRSWVCFSVSWVRWRVVVWWFAVAPPFCH 317
>gb|CO514341.1|CO514341 s13dSG84D0400030_157344 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 534
Score = 27.2 bits (53), Expect = 4.8
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -2 / +2
Query: 375 SRQHPRSWTKFL*RLTSNWNL 313
S QH R WT L ++ +NW+L
Sbjct: 119 SYQHCRHWTCRLWKINNNWSL 181
>gb|CO515361.1|CO515361 s13dSG49C0400034_417781 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 572
Score = 24.9 bits (48), Expect(2) = 5.3
Identities = 15/37 (40%), Positives = 20/37 (54%)
Frame = -2 / -2
Query: 984 LSLLSSLRHRTANMPIMSRQRLLHRD*ASSPEKSSLL 874
LSL SSL + P +R + R+ A+S EKS L
Sbjct: 538 LSLSSSLIQKVFQAPRAQPRRYILRNPANSREKSGQL 428
Score = 21.7 bits (41), Expect(2) = 5.3
Identities = 5/12 (41%), Positives = 7/12 (58%)
Frame = -1 / -2
Query: 688 WVHVAYICSHCP 653
W H+ +C CP
Sbjct: 172 WNHIVSLCISCP 137
>gb|CO512859.1|CO512859 s13dSG20E1000071_121644 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 486
Score = 24.4 bits (47), Expect(2) = 6.1
Identities = 11/34 (32%), Positives = 17/34 (50%)
Frame = +2 / +3
Query: 335 RHKNLVQLLGCCLETDIPILVFEFVANGSLEDIL 436
RH N++ +G +V EF+ GSL +L
Sbjct: 360 RHPNILLFMGAVTSPQRLCIVTEFLPRGSLFRLL 461
Score = 21.7 bits (41), Expect(2) = 6.1
Identities = 5/9 (55%), Positives = 6/9 (66%)
Frame = +1 / +2
Query: 127 WRRALWQCL 153
W R LW C+
Sbjct: 221 WARVLWNCI 247
>gb|CO513901.1|CO513901 s13dSG73H0500044_156464 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 558
Score = 26.7 bits (52), Expect = 6.6
Identities = 7/17 (41%), Positives = 12/17 (70%)
Frame = -2 / +1
Query: 279 LCCGSGTRRGRIKFFQW 229
+CCGSGT+ + ++W
Sbjct: 427 VCCGSGTK*AEVARYKW 477
>gb|CO514107.1|CO514107 s13dSG75D0400030_156876 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 454
Score = 26.7 bits (52), Expect = 6.6
Identities = 9/25 (36%), Positives = 13/25 (52%)
Frame = -3 / -2
Query: 935 CRGNACCIEIEHPRLRNQVCYHTSH 861
C +AC + ++ VC HTSH
Sbjct: 252 CLASACSLHFTSDVVQINVCMHTSH 178
>gb|L01660.1|ALFNADHSYN Medicago sativa NADH-glutamate synthase mRNA, comlete cds
Length = 7174
Score = 26.7 bits (52), Expect = 6.6
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +3 / -1
Query: 471 RSGWTSPLAPRKLSPTC 521
R WT+P A R+L+ TC
Sbjct: 2500 RCWWTAPTAKRELTATC 2450
>emb|AJ401228.1|MSA401228 Medicago sativa mRNA for selenium binding protein (sbp gene)
Length = 1776
Score = 26.7 bits (52), Expect = 6.6
Identities = 11/29 (37%), Positives = 16/29 (55%)
Frame = -2 / +1
Query: 312 ISFMNDPSSSTLCCGSGTRRGRIKFFQWQ 226
I F++DP+ T GS I+FF+ Q
Sbjct: 952 IRFLHDPAKDTGFVGSALTSNMIRFFKTQ 1038
>emb|X97316.1|MSCDC2MSE M.sativa mRNA for cdc2 kinase homologue, cdc2MsE
Length = 1490
Score = 26.7 bits (52), Expect = 6.6
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +2 / +3
Query: 545 VHGDIKPSNILLDDD 589
VH D+KPSNIL+ D
Sbjct: 288 VHRDVKPSNILVMGD 332
>gb|DQ122809.1| Medicago sativa clone P24 nuclear pore complex protein-related
protein mRNA, partial cds
Length = 630
Score = 23.5 bits (45), Expect(2) = 7.7
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = +3 / +2
Query: 429 TYFIVPRNHVPSRCRSGWTSPLAPRKLS 512
TY ++ +NH P + S + RKLS
Sbjct: 311 TYLVLTKNHCPKQSYSLSLNLTTSRKLS 394
Score = 22.6 bits (43), Expect(2) = 7.7
Identities = 9/17 (52%), Positives = 10/17 (58%)
Frame = +2 / +1
Query: 89 ELKKITNGYSKRLGGGH 139
+LKKI N RLG H
Sbjct: 154 QLKKIINDQHTRLGDAH 204
>emb|X59930.1|MFSTRESS M.falcata mRNA for environmental stress and abscisic acid inducible
protein
Length = 679
Score = 24.0 bits (46), Expect(2) = 8.2
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = +2 / +1
Query: 710 FTLECDVYSFGVVLLELITRRRASWY 787
+ L C VYS V L+E++ + + +Y
Sbjct: 586 YYLSCHVYSIDVSLVEIMYKFASIYY 663
Score = 22.1 bits (42), Expect(2) = 8.2
Identities = 6/10 (60%), Positives = 7/10 (70%)
Frame = +1 / +3
Query: 655 GSGCRYRLHG 684
G GC+Y HG
Sbjct: 393 GGGCQYHCHG 422
>gb|AW698364.1|AW698364 G210 glandular-haired subtracted cDNA library Medicago sativa cDNA,
mRNA sequence
Length = 129
Score = 26.3 bits (51), Expect = 9.1
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -1 / -3
Query: 1183 IIACISTAAMFSASHVLTDHSCCLLRF 1103
++ CI+T + FS + +S C+L F
Sbjct: 91 LLDCITTYSPFSQDYTQHQYSVCILNF 11
>gb|CO512338.1|CO512338 s13dSG68G0800056_114190 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 593
Score = 26.3 bits (51), Expect = 9.1
Identities = 12/36 (33%), Positives = 17/36 (47%)
Frame = +2 / +2
Query: 140 FGNVYEGTIVDGRKVAVKCPLRTRVSSHRCHWKNLI 247
F + G I + V+CP +SSH + K LI
Sbjct: 83 FSSFTAGNISWVSSIKVQCPFHILISSHHNYIKGLI 190
>gb|CO515693.1|CO515693 s13dSG60F0900079_419535 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 568
Score = 26.3 bits (51), Expect = 9.1
Identities = 11/22 (50%), Positives = 13/22 (59%)
Frame = -3 / +3
Query: 143 QSALLQGVCCSH*LSSSAPFWC 78
QS L CCS S+S+ FWC
Sbjct: 225 QSKCLVFFCCSINSSASSIFWC 290
>gb|CO515884.1|CO515884 s13dSG91C1100084_421307 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 551
Score = 26.3 bits (51), Expect = 9.1
Identities = 13/23 (56%), Positives = 14/23 (60%)
Frame = +2 / +1
Query: 554 DIKPSNILLDDDLNPKVSDFGSS 622
DIK NILLDDD K F +S
Sbjct: 370 DIKLQNILLDDDFCQKNLRFWTS 438
>gb|CO516320.1|CO516320 s13dSG99A0700051_445968 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 559
Score = 26.3 bits (51), Expect = 9.1
Identities = 7/13 (53%), Positives = 10/13 (76%)
Frame = +1 / +3
Query: 535 SKACPWRHQAFQH 573
SKACPW+ ++H
Sbjct: 381 SKACPWKEGPYRH 419
>gb|AF505625.1| Medicago sativa putative basic helix-loop-helix protein mRNA, partial
cds
Length = 653
Score = 26.3 bits (51), Expect = 9.1
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = -1 / -2
Query: 1294 LRSIKRSNSCELLCLQIDIAVLLEE*IMYIYL 1199
LR +K S SC LLC I + *++++ L
Sbjct: 436 LRDLKSSLSCSLLCKPISSELSSTP*LVFLVL 341
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: May 2, 2006 2:40 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,322,406
Number of Sequences: 7669
Number of extensions: 180301
Number of successful extensions: 1111
Number of sequences better than 10.0: 66
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 368097328
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)