TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.451
(1101 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514693.1|CO514693 s13dSG46H1100096_328046 Glandular tr... 194 7e-086
gb|CO514795.1|CO514795 s13dSG59C0900070_328250 Glandular tr... 188 8e-059
>gb|CO514693.1|CO514693 s13dSG46H1100096_328046 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 552
Score = 194 bits (418), Expect(2) = 7e-086
Identities = 77/85 (90%), Positives = 81/85 (95%)
Frame = +2 / +3
Query: 92 TMKFNIANPSTGCQKKLEIDDDQKLRAFYDRRISQEVSGDALGEEFKGYVFKIMGGCDKQ 271
TMKFN+ANP+TGCQKKLEIDDD KLRAF+D+RISQEV GDALGEEFKGYVFKI GGCDKQ
Sbjct: 63 TMKFNVANPTTGCQKKLEIDDDMKLRAFWDKRISQEVLGDALGEEFKGYVFKITGGCDKQ 242
Query: 272 GFPMKQGVLTSGRVRLLLHRGTPCF 346
GFPMKQGVLT GRVRLLLHRGTPCF
Sbjct: 243 GFPMKQGVLTPGRVRLLLHRGTPCF 317
Score = 190 bits (410), Expect(2) = 7e-054
Identities = 85/132 (64%), Positives = 93/132 (70%)
Frame = -1 / -3
Query: 486 FSVSVRPGRSFSPFFTITKLITDRSWLTMQPRTDFXXXXXXXXXXXRKQGVPLWSRRRTR 307
FS SV+PG+SFSPFFTITK T+RS LTM PRTDF RKQGVPLWS+RRTR
Sbjct: 457 FSTSVKPGKSFSPFFTITKFRTERSGLTMHPRTDFLLLSPLRLPCPRKQGVPLWSKRRTR 278
Query: 306 PEVKTPCFIGKPCLSHPPMILKT*PLNSSPRASPLTSWDILRS*KARXXXXXXXXXXXWH 127
P V TPCFIG PCLSHPP+ILKT PLNSSP+ASP TS +IL S AR W
Sbjct: 277 PGVNTPCFIGNPCLSHPPVILKTYPLNSSPKASPKTSCEILLSQNARSFMSSSISSFFWQ 98
Query: 126 PVEGFAMLNFMV 91
PV GFA LNF+V
Sbjct: 97 PVVGFATLNFIV 62
Score = 174 bits (375), Expect = 1e-044
Identities = 84/162 (51%), Positives = 107/162 (66%)
Frame = -3 / -2
Query: 580 VCVHVLAYIIILVKVEQLSDLGGPLGTPHPWLLSVSQTRQIILTLLHNHQVDNR*VLAHD 401
V V +L IIIL K+E+L+D G L TPH WL ++SQT QII TLLHN+QV NR V A +
Sbjct: 551 VGVDILPDIIILGKIEELTDFGSSLRTPHSWLFNISQTWQIIFTLLHNNQVQNREVWADN 372
Query: 400 ATTDRXXXXXXXXXXXXTEAGSAPMEQKTNTTRSQNSLLHWEALLVTSTHDLEDITLELL 221
A T+R +E S +EQ+ NTTR ++LLHW++L VTST D EDI+L+
Sbjct: 371 APTNRLPSSLSITPSVSSETRSTSVEQEANTTRG*HTLLHWKSLFVTSTSDFEDISLKFF 192
Query: 220 TQSITTDLLGYPSVIKGTQLLVVIDFQLLLAPGGRVRDVELH 95
SIT +LL P V K T+L VVIDF+LLLA G + +VELH
Sbjct: 191 A*SITQNLL*DPLVPKCTKLHVVIDFKLLLATSGWICNVELH 66
Score = 138 bits (296), Expect(2) = 7e-086
Identities = 57/61 (93%), Positives = 59/61 (96%)
Frame = +2 / +3
Query: 398 CIVSQDLSVINLVIVKKGENDLPGLTDTEKPRMRGPKRASKIRKLFNLNKDDDVRKYVNT 577
CIVS DLSV+NLVIVKKGENDLPGLTD EKPRMRGPKRASKIRKLFNL+KDDDVRKYVNT
Sbjct: 369 CIVSPDLSVLNLVIVKKGENDLPGLTDVEKPRMRGPKRASKIRKLFNLSKDDDVRKYVNT 548
Query: 578 Y 580
Y
Sbjct: 549 Y 551
Score = 91.3 bits (193), Expect(2) = 2e-037
Identities = 42/86 (48%), Positives = 56/86 (65%)
Frame = +1 / +2
Query: 91 HHEVQHREPFHRVPKEAGNR*RPEAACLL*PKDIPGGQW*CSG*GVQGLCLQDHGWM*QA 270
H+EVQ + H +P+EA NR*R EA+C+L +D+ G *C + G+CLQ+H WM*Q
Sbjct: 62 HNEVQRCKSNHWLPEEA*NR*RHEASCILGQEDLTRGFG*CFRRRI*GICLQNHWWM*QT 241
Query: 271 RLPNEARSFDFWSCSSSAP*GHSLLP 348
R+ NEA + WSCS AP +SL P
Sbjct: 242 RISNEAGCVNPWSCSPLAPQRYSLFP 319
Score = 79.4 bits (167), Expect(2) = 2e-037
Identities = 36/65 (55%), Positives = 48/65 (73%)
Frame = +1 / +2
Query: 385 VCPWLHREPRPICYQLGDCEEG*E*SAWSD*H*EAKDEGSQEGLQDQKAVQP*QG**CTQ 564
VC W+H +PRP+C +LG CEEG*+* A D* *+AK+EGS E Q+ A+Q QG**C +
Sbjct: 356 VCSWVHCQPRPLCSELGYCEEG*K*FARFD*C*KAKNEGS*ESFQNP*ALQSFQG**CQE 535
Query: 565 VREHI 579
V +H+
Sbjct: 536 VCQHL 550
Score = 35.4 bits (71), Expect(2) = 7e-054
Identities = 17/34 (50%), Positives = 19/34 (55%)
Frame = -2 / -1
Query: 581 GMCSRTCVHHHPC*G*TAF*SWRPSWDPSSLASQ 480
G C T HHHP * A+ W+ S DPS LA Q
Sbjct: 552 GRC*HTS*HHHPWKD*RAYGFWKLS*DPSFLAFQ 451
>gb|CO514795.1|CO514795 s13dSG59C0900070_328250 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 449
Score = 188 bits (406), Expect(2) = 8e-059
Identities = 75/84 (89%), Positives = 79/84 (94%)
Frame = +2 / +2
Query: 95 MKFNIANPSTGCQKKLEIDDDQKLRAFYDRRISQEVSGDALGEEFKGYVFKIMGGCDKQG 274
MK N+ANP+TGCQKKLEIDDD KLRAF+D+RISQEV GDALGEEFKGYVFKI GGCDKQG
Sbjct: 68 MKVNVANPTTGCQKKLEIDDDMKLRAFWDKRISQEVLGDALGEEFKGYVFKITGGCDKQG 247
Query: 275 FPMKQGVLTSGRVRLLLHRGTPCF 346
FPMKQGVLT GRVRLLLHRGTPCF
Sbjct: 248 FPMKQGVLTPGRVRLLLHRGTPCF 319
Score = 173 bits (373), Expect = 3e-044
Identities = 82/140 (58%), Positives = 89/140 (63%)
Frame = -1 / -3
Query: 474 VRPGRSFSPFFTITKLITDRSWLTMQPRTDFXXXXXXXXXXXRKQGVPLWSRRRTRPEVK 295
V PGRS SPF ITK T+RS LTM PRTDF RKQGVPLWS+ TRP V
Sbjct: 447 VSPGRSLSPFLPITKFKTERSGLTMHPRTDFLXRSPLRLPCPRKQGVPLWSKSLTRPGVN 268
Query: 294 TPCFIGKPCLSHPPMILKT*PLNSSPRASPLTSWDILRS*KARXXXXXXXXXXXWHPVEG 115
TPCFIG PCLSHPP+ILKT*PLNSSP+ASP TS +IL S KAR HPV G
Sbjct: 267 TPCFIGNPCLSHPPVILKT*PLNSSPKASPRTSCEILLSQKARSFMSSSISSFF*HPVVG 88
Query: 114 FAMLNFMVALRRALAGSEEL 55
A L FM+ R +E L
Sbjct: 87 LATLTFMLESRPLDPAAETL 28
Score = 129 bits (276), Expect = 7e-031
Identities = 64/127 (50%), Positives = 84/127 (66%)
Frame = -3 / -2
Query: 475 SQTRQIILTLLHNHQVDNR*VLAHDATTDRXXXXXXXXXXXXTEAGSAPMEQKTNTTRSQ 296
S+ +II+T L N+QV NR V A +A+T+R TE S+ MEQ+ NT R Q
Sbjct: 448 SKPWKIIITFLTNNQVQNREVGADNASTNRLPXTLSIAPSVSTETRSSSMEQEPNTARGQ 269
Query: 295 NSLLHWEALLVTSTHDLEDITLELLTQSITTDLLGYPSVIKGTQLLVVIDFQLLLAPGGR 116
+SLLHWE+L VT T DLEDITL+L +QSIT + L P V + T+L V+IDF+LLL G
Sbjct: 268 HSLLHWESLFVTPTSDLEDITLKLFSQSITKNFL*DPLVPESTELHVIIDFKLLLTSSGW 89
Query: 115 VRDVELH 95
+ +V LH
Sbjct: 88 IGNVNLH 68
Score = 128 bits (275), Expect = 9e-031
Identities = 56/114 (49%), Positives = 70/114 (61%)
Frame = +1 / +1
Query: 94 HEVQHREPFHRVPKEAGNR*RPEAACLL*PKDIPGGQW*CSG*GVQGLCLQDHGWM*QAR 273
HE + H + +EA NR* EA C L +D+ G W*C G V GLCLQDH W+*Q R
Sbjct: 67 HEG*RCQSNHWMSEEA*NR**HEAPCFLGQEDLTGSSW*CFGRRV*GLCLQDHWWV*QTR 246
Query: 274 LPNEARSFDFWSCSSSAP*GHSLLPWLXXXXXXXXXXVCPWLHREPRPICYQLG 435
+PNEA S D W C + AP* +SL PW VC W+H +PRP+C++LG
Sbjct: 247 IPNEAGSVDPWPC*ALAP*RNSLFPWTRKAQWRAXKEVCSWMHCQPRPLCFELG 408
Score = 53.8 bits (111), Expect(2) = 8e-059
Identities = 22/26 (84%), Positives = 24/26 (92%)
Frame = +2 / +2
Query: 398 CIVSQDLSVINLVIVKKGENDLPGLT 475
CIVS DLSV+NLVI KKG+NDLPGLT
Sbjct: 371 CIVSPDLSVLNLVIGKKGDNDLPGLT 448
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,664,943
Number of Sequences: 7669
Number of extensions: 93129
Number of successful extensions: 827
Number of sequences better than 1.0e-020: 3
length of database: 1,248,568
effective HSP length: 43
effective length of database: 918,801
effective search space used: 296772723
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)