TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.334
(1733 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO512532.1|CO512532 s13dSG08C0200006_120990 Glandular tr... 343 4e-095
>gb|CO512532.1|CO512532 s13dSG08C0200006_120990 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 579
Score = 343 bits (743), Expect = 4e-095
Identities = 132/150 (88%), Positives = 141/150 (94%)
Frame = -1 / +1
Query: 1580 EVFFQEKFEDGWESRWVKSEWKKDENMAGEWNHTSGKWNGDAEDKGIQTSEDYRFYAISA 1401
+VFF+E+FEDGWESRWVKSEWKKDEN+AGEWN+TSG+WNGDA DKGIQTSEDYRFYAISA
Sbjct: 130 KVFFEERFEDGWESRWVKSEWKKDENLAGEWNYTSGQWNGDANDKGIQTSEDYRFYAISA 309
Query: 1400 EYPEFSNKDKTLVLQFSVKHEQKLDCGGGYVKLLGGDVDQKKFGGDTSYSIMFGPDICGY 1221
E+PEFSNKD TLV QFSVKHEQKLDCGGGY+KLL GDVDQKKFGGDT YSIMFGPDICGY
Sbjct: 310 EFPEFSNKDNTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICGY 489
Query: 1220 STKKVHTILTKDGKNHLIKKDVPCETDQLT 1131
STKKVH ILT + NHLIKKDVPCETDQLT
Sbjct: 490 STKKVHAILTYNDTNHLIKKDVPCETDQLT 579
Score = 167 bits (359), Expect = 4e-042
Identities = 77/162 (47%), Positives = 101/162 (62%)
Frame = +3 / -1
Query: 1131 SQLISLTRHILLDQVVLAILGQDSVNLLGAVPTDIWSKHNAVRCVSTKFLLVYITTQQFD 1310
S+L+SLTR+ILLD VV I+GQ+SV G V TDIW+KH+ V + KFLL+ I +QF
Sbjct: 579 SKLVSLTRNILLD*VVCVIVGQNSVYFFGTVTTDIWTKHDTVGSIPAKFLLINIAAKQFH 400
Query: 1311 XXXXXXXXXXXXHRELQHQGLILVAELRVFG*NGIEPVILGGLNTFVLGISIPFSRSVVP 1490
R+L+ +ILVAE R +GI+ VI GLNTFV+ ++IP +R VVP
Sbjct: 399 VTTTTVKFLFMLDRKLED*CVILVAEFRELSRDGIKSVIFTGLNTFVISVTIPLTRGVVP 220
Query: 1491 FTSHVLILLPLGLDPPTFPAIFELLLEEDLPCDGGDRGEREK 1616
VLILLP PP FP+IFE L EE+L G +GE+ +
Sbjct: 219 LPGQVLILLPFRFYPPAFPSIFEALFEENLRRSDG*KGEKNE 94
Score = 141 bits (303), Expect(2) = 2e-053
Identities = 66/81 (81%), Positives = 66/81 (81%)
Frame = +1 / -2
Query: 1333 FCSCFTENCSTRVLSLLLNSGYSAEMA*NL*SSEV*IPLSSASPFHFPEVWFHSPAMFSS 1512
FCSC TEN T VLSLLLNSG SAEMA*NL*SS V*IPLS ASPFH PEV FHSPA FSS
Sbjct: 377 FCSCLTENWKTSVLSLLLNSGNSAEMA*NL*SSLV*IPLSLASPFH*PEV*FHSPAKFSS 198
Query: 1513 FFHSDLTHRLSQPSSNFSWKK 1575
FFHSD THRLS PSS S KK
Sbjct: 197 FFHSDFTHRLSHPSSKRSSKK 135
Score = 85.8 bits (181), Expect(2) = 3e-030
Identities = 33/61 (54%), Positives = 39/61 (63%)
Frame = +2 / -3
Query: 1373 YPCC*TQGIRLKWHRTCNPRRFEYLCPRHLHSIFPKCGSIHQPCSHPSSTRT*PTDFPSH 1552
YPCC* QG + +WH+ CN FEYLC HSI +C S +P SHPSS + PT FP H
Sbjct: 337 YPCC*IQGTQQRWHKICNLHWFEYLCH*RHHSIDQRCSSTPRPSSHPSSIQILPTGFPIH 158
Query: 1553 L 1555
L
Sbjct: 157 L 155
Score = 83.5 bits (176), Expect(2) = 2e-053
Identities = 38/50 (76%), Positives = 42/50 (84%)
Frame = +1 / -2
Query: 1132 VN*SVSQGTSFLIKWFLPSLVRIV*TFLVLYPQISGPNIML*DVSPPNFF 1281
V+ SVSQGTSFLIKWF+ VRI TFLVL PQISGPN++L* VSPPNFF
Sbjct: 578 VSWSVSQGTSFLIKWFVSL*VRIACTFLVL*PQISGPNMIL*GVSPPNFF 429
Score = 73.5 bits (154), Expect(2) = 2e-029
Identities = 35/61 (57%), Positives = 42/61 (68%)
Frame = -2 / +2
Query: 1555 KMAGKVGGSSPSGRRMRTWLVNGTTLRENGMEMPRTKVFKPPRITGSMPFQPNTLSSATR 1376
KM GK GG + +GRRMRTW +GTT NGM TKVFKP +IT MP ++L+SATR
Sbjct: 155 KMDGKAGG*NLNGRRMRTWPGSGTTPLVNGMVTLMTKVFKPVKITDFMPSLLSSLNSATR 334
Query: 1375 I 1373
I
Sbjct: 335 I 337
Score = 71.6 bits (150), Expect(2) = 2e-029
Identities = 27/50 (54%), Positives = 38/50 (76%)
Frame = -3 / +3
Query: 1290 CRPEEIWWRHILQHYVWTRYLWVQHQEGSHYPDQGWQEPLDQEGCAL*D* 1141
C EEIW + LQ++VW+RYLW+Q+Q+ + Y D +PL+QEGC+L*D*
Sbjct: 420 C*SEEIWRGYSLQYHVWSRYLWLQYQKSTRYSDLQ*HKPLNQEGCSL*D* 569
Score = 71.6 bits (150), Expect(2) = 2e-027
Identities = 33/60 (55%), Positives = 41/60 (68%)
Frame = -3 / +3
Query: 1551 WLGKSVGQVRVEEG*EHGW*MEPHFGKMEWRCRGQRYSNLRGLQVLCHFSRIP*VQQQG* 1372
W+GK VG++ +EEG*E G +E H MEW QRYSN LQ+LCH +P*+QQQG*
Sbjct: 159 WMGKPVGKI*MEEG*ELGRGVELHLWSMEW*R**QRYSNQ*RLQILCHLC*VP*IQQQG* 338
Score = 66.1 bits (138), Expect(2) = 2e-027
Identities = 33/57 (57%), Positives = 37/57 (64%)
Frame = -2 / +2
Query: 1306 NCWVVM*TRRNLVETHLTALCLDQISVGTAPRRFTLS*PRMARTT*SRRMCLVRLIS 1136
NC M RRNL T CL QISV T P+++TL *P M +TT*SRRM LVRL S
Sbjct: 404 NCLAAMLIRRNLAGILPTVSCLVQISVVTVPKKYTLF*PTMTQTT*SRRMFLVRLTS 574
Score = 62.0 bits (129), Expect(2) = 3e-030
Identities = 31/55 (56%), Positives = 32/55 (58%)
Frame = +2 / -3
Query: 1142 QSHKAHPS*SSGSCHPWSG*CEPSWCCTHRYLVQT*CCKMCLHQISSGLHHHPAI 1306
QSHK HPS* SG CH S * W C HRYL QT* C+ QISS H AI
Sbjct: 568 QSHKEHPS*LSGLCHCRSE*RVLFWYCNHRYLDQT*YCREYPRQISSDQHRR*AI 404
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,620,647
Number of Sequences: 7669
Number of extensions: 180523
Number of successful extensions: 979
Number of sequences better than 1.0e-020: 1
length of database: 1,248,568
effective HSP length: 44
effective length of database: 911,132
effective search space used: 485633356
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)