TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.287
(857 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular tr... 192 5e-050
gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular tr... 189 3e-049
gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular tr... 158 8e-040
gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular tr... 133 4e-032
gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular tr... 121 1e-028
>gb|CO514287.1|CO514287 s13dSG76F1000079_157236 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 521
Score = 192 bits (414), Expect = 5e-050
Identities = 82/89 (92%), Positives = 87/89 (97%)
Frame = +1 / +2
Query: 262 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 441
VETYKIYIFKVLKQVHPDIG+SSKAM IMNSFINDIFEKLA E+++LARYNKKPTITSRE
Sbjct: 209 VETYKIYIFKVLKQVHPDIGVSSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSRE 388
Query: 442 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 528
IQT+VRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 389 IQTAVRLVLPGELAKHAVSEGTKAVTKFT 475
Score = 105 bits (224), Expect = 8e-024
Identities = 53/89 (59%), Positives = 61/89 (68%)
Frame = -1 / -3
Query: 533 EEVNLVTALVPSETACLASSPGSTRRTEVWISRDVMVGFXXXXXXXXXXXXXXSKMSLMK 354
E VNLVTALVPSETACLA+SPG T T V IS +V+VGF SK+SLMK
Sbjct: 480 ELVNLVTALVPSETACLANSPGRTNLTAV*ISLEVIVGFLLYLAKRDDS*ASFSKISLMK 301
Query: 353 EFMMDMALEEMPMSGCTCFSTLKM*ILYV 267
F++ +AL E P+SG TCF TLKM ILYV
Sbjct: 300 LFIIPIALLETPISG*TCFRTLKMYILYV 214
>gb|CO513351.1|CO513351 s13dSG38A0300017_129866 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 566
Score = 189 bits (408), Expect = 3e-049
Identities = 82/89 (92%), Positives = 86/89 (96%)
Frame = +1 / +1
Query: 262 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 441
VETYKIYIFK LKQVHPDIGISSKAM IMNSFINDIFEKLA E+++LARYNKKPTITSRE
Sbjct: 247 VETYKIYIFKGLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSRE 426
Query: 442 IQTSVRLVLPGELAKHAVSEGTKAVTKFT 528
IQT+VRLVLPGELAKHAVSEGTKAVTKFT
Sbjct: 427 IQTAVRLVLPGELAKHAVSEGTKAVTKFT 513
Score = 114 bits (243), Expect = 2e-026
Identities = 57/87 (65%), Positives = 62/87 (71%)
Frame = -1 / -1
Query: 533 EEVNLVTALVPSETACLASSPGSTRRTEVWISRDVMVGFXXXXXXXXXXXXXXSKMSLMK 354
E VNLVTALVPSETACLASSPG TRRT V ISR+VMVGF SK+SLMK
Sbjct: 518 ELVNLVTALVPSETACLASSPGRTRRTAV*ISREVMVGFLLYLASLEDS*ASFSKISLMK 339
Query: 353 EFMMDMALEEMPMSGCTCFSTLKM*IL 273
FM+ +AL E+PMSG TC LKM*IL
Sbjct: 338 LFMIPIALLEIPMSG*TCLRPLKM*IL 258
>gb|CO516018.1|CO516018 s13dSG64A0200005_445364 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 500
Score = 158 bits (340), Expect = 8e-040
Identities = 68/74 (91%), Positives = 72/74 (97%)
Frame = +1 / +2
Query: 262 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTITSRE 441
VETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIFEKLA E+++LARYNKKPTITSRE
Sbjct: 263 VETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTITSRE 442
Query: 442 IQTSVRLVLPGELA 483
IQT+VRLVLPGELA
Sbjct: 443 IQTAVRLVLPGELA 484
>gb|CO516125.1|CO516125 s13dSG65F0900079_445578 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 363
Score = 133 bits (284), Expect = 4e-032
Identities = 57/62 (91%), Positives = 61/62 (98%)
Frame = +1 / +2
Query: 343 IMNSFINDIFEKLAGEAAKLARYNKKPTITSREIQTSVRLVLPGELAKHAVSEGTKAVTK 522
IMNSFINDIFEKLA E+++LARYNKKPTITSREIQT+VRLVLPGELAKHAVSEGTKAVTK
Sbjct: 8 IMNSFINDIFEKLAQESSRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTK 187
Query: 523 FT 528
FT
Sbjct: 188 FT 193
>gb|CO516115.1|CO516115 s13dSG65E0500039_445558 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 403
Score = 121 bits (259), Expect = 1e-028
Identities = 51/56 (91%), Positives = 54/56 (96%)
Frame = +1 / +2
Query: 262 VETYKIYIFKVLKQVHPDIGISSKAMSIMNSFINDIFEKLAGEAAKLARYNKKPTI 429
VETYKIYIFKVLKQVHPDIGISSKAM IMNSFINDIFEKLA E+++LARYNKKPTI
Sbjct: 236 VETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQESSRLARYNKKPTI 403
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 4,264,086
Number of Sequences: 7669
Number of extensions: 43317
Number of successful extensions: 586
Number of sequences better than 1.0e-020: 5
length of database: 1,248,568
effective HSP length: 42
effective length of database: 926,470
effective search space used: 225132210
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)