TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 131537.2.202
(3202 letters)
Database: MedicagoSativa_nucl_with_EST.fasta
7669 sequences; 3,745,706 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRN... 1446 0.0
gb|DQ122788.1| Medicago sativa clone B11 sucrose synthase m... 236 2e-081
gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular tr... 208 8e-075
gb|CO515699.1|CO515699 s13dSG60G0600052_419547 Glandular tr... 273 7e-074
gb|CO517054.1|CO517054 s13dSG31G0500037_468654 Glandular tr... 243 9e-065
gb|DQ122842.1| Medicago sativa clone MF8 sucrose synthase m... 180 1e-045
>gb|AF049487.1|AF049487 Medicago sativa sucrose synthase mRNA, complete cds
Length = 2760
Score = 1446 bits (3151), Expect(2) = 0.0
Identities = 587/752 (78%), Positives = 658/752 (87%)
Frame = -2 / +2
Query: 2802 IPEAEREKLKDGAFEDVLRAAQEAIVIPPWVALAIRPRPGVWEYVRVNVSELAVEELRVP 2623
IPE R+KL DGAF +VLR+ QEAIV+PPWVALA+RPRPG+WEY+RVNV L VE L+
Sbjct: 221 IPEESRQKLTDGAFGEVLRSTQEAIVLPPWVALAVRPRPGIWEYLRVNVHALVVENLQPA 400
Query: 2622 EYLQFKEQLVEEGPNNNFVLELDFEPFNASFPRPSLSKSIGNGVQFLNRHLSSKLFHDKE 2443
E+L+FKE+LV+ N NFVLELDFEPF ASFPRP+L+KSIGNGV FLNRHLS+KLFHDKE
Sbjct: 401 EFLKFKEELVDGSANGNFVLELDFEPFTASFPRPTLNKSIGNGVHFLNRHLSAKLFHDKE 580
Query: 2442 SMYPLLNFLRAHNYKGMTMMLNDRIRSLSALQGALRKAEEHLSTLQADTPYSEFHHRFQE 2263
S++PLL FLR H+YKG T+MLNDRI++ +LQ LRKAEE+LST+ +TPYSEF HRFQE
Sbjct: 581 SLHPLLEFLRLHSYKGKTLMLNDRIQNPDSLQHVLRKAEEYLSTIDPETPYSEFEHRFQE 760
Query: 2262 LGLEKGWGDCAKRAQETIHLLLDLLEAPDPSTLEKFLGTIPMVFNVVILSPHGYFAQANV 2083
+GLE+GWGD A+R E+I LLLDLLEAPDP TLE FL IPMVFNVVILSPHGYFAQ +V
Sbjct: 761 IGLERGWGDTAERVLESIQLLLDLLEAPDPCTLESFLDRIPMVFNVVILSPHGYFAQDDV 940
Query: 2082 LGYPDTGGQVVYILDQVRAMENEMLLRIKQCGLDITPKILIVTRLLPDATGTTCGQRLEK 1903
LGYPDTGGQVVYILDQVRA+E+EML RIK+ GLDI P+ILI+TRLLPDA GTTCGQRLEK
Sbjct: 941 LGYPDTGGQVVYILDQVRALESEMLSRIKKQGLDIIPRILIITRLLPDAVGTTCGQRLEK 1120
Query: 1902 VLGTEHCHILRVPFRTENGIVRKWISRFEVWPYLETYTDDVAHEIAGELQANPDLIIGNY 1723
V GTEHCHILRVPFR E GIVRKWISRFEVWPYLETYT+DVAHE+A ELQ+ PDLI+GNY
Sbjct: 1121 VYGTEHCHILRVPFRDEKGIVRKWISRFEVWPYLETYTEDVAHELAKELQSKPDLIVGNY 1300
Query: 1722 SDGNLVACLLAHKMGVTHCTIAHALEKTKYPNSDLYWKKFEDHYHFSCQFTTDLIAMNHA 1543
SDGN+VA LLAHK+GVT CTIAHALEKTKYP SD+YWKKFE+ YHFSCQFT DL AMNH
Sbjct: 1301 SDGNIVASLLAHKLGVTQCTIAHALEKTKYPESDIYWKKFEEKYHFSCQFTADLFAMNHT 1480
Query: 1542 DFIITSTFQEIAGNKDTVGQYESHMAFTMPGLYRVVHGIDVFDPKFNIVSPGADLSIYFP 1363
DFIITSTFQEIAG+KD VGQYESH AFT+PGLYRVVHGIDVFDPKFNIVSPGAD +IYFP
Sbjct: 1481 DFIITSTFQEIAGSKDKVGQYESHTAFTLPGLYRVVHGIDVFDPKFNIVSPGADQTIYFP 1660
Query: 1362 YTESHKRLTSLHPEIEELLYSQTENTEHKFVLNDRNKPIIFSMARLDRVKNLTGLVELYG 1183
YTE+ +RLTS +PEIEELLYS EN EH VL DRNKPIIF+MARLDRVKN+TGLVE YG
Sbjct: 1661 YTETSRRLTSFYPEIEELLYSSVENEEHICVLKDRNKPIIFTMARLDRVKNITGLVEWYG 1840
Query: 1182 RNKRLQELVNLVVVCGDHGNPSKDKEEQAEFKKMFDLIEQYNLNGHIRWISAQMNRVRNG 1003
+N +L+ELVNLVVV GD SKD EE AE KKM+ LIE Y LNG RWIS+QMNRVRNG
Sbjct: 1841 KNAKLRELVNLVVVAGDRRKESKDLEEIAEMKKMYGLIETYKLNGQFRWISSQMNRVRNG 2020
Query: 1002 ELYRYICDTKGAFVQPAFYEAFGLTVVEAMTCGLPTFATAYGGPAEIIVHGVSGYHIDPY 823
ELYR ICDTKGAFVQPA YEAFGLTVVEAM GLPTFAT GGPAEIIVHG SG+HIDPY
Sbjct: 2021 ELYRVICDTKGAFVQPAVYEAFGLTVVEAMATGLPTFATLNGGPAEIIVHGKSGFHIDPY 2200
Query: 822 QGDKASALLVDFFDKCQAEPSHWSKISQGGLQRIEEKYTWKLYSERLMTLTGVYGFWKYV 643
GD+A+ LLV+FF+K +A+PSHW KISQGGLQRIEEKYTW +YS+RL+TLTGVYGFWK+V
Sbjct: 2201 HGDRAADLLVEFFEKVKADPSHWDKISQGGLQRIEEKYTWTIYSQRLLTLTGVYGFWKHV 2380
Query: 642 SNLERRETRRYLEMLYALKYRTMASTVPLAVE 547
SNL+R E+RRYLEM YALKYR +A +VPLAVE
Sbjct: 2381 SNLDRLESRRYLEMFYALKYRKLAESVPLAVE 2476
Score = 930 bits (2025), Expect(3) = 0.0
Identities = 465/722 (64%), Positives = 505/722 (69%)
Frame = +2 / -1
Query: 641 DTYFQNPYTPVRVISLSEYSFQVYFSSIRWSPPWEILLQWLGSAWHLSKKSTSRADALSP 820
DT FQ P TPV V SL E VYFSSIRW PP EIL Q GSA SK ST R+ A SP
Sbjct: 2382 DTCFQKP*TPVSVRSL*E*IVHVYFSSIRWRPP*EILSQ*DGSALTFSKNSTRRSAARSP 2203
Query: 821 W*GSMW*PDTPCTMISAGPP*AVANVGRPQVMASTTVSPKAS*KAGCTKAPLVSQM*RYS 1000
W*GSMW PD P TMISAGPP VANVG P MASTTV PKAS AGCTKAPLVSQ+ RYS
Sbjct: 2202 W*GSMWNPDLPWTMISAGPPLRVANVGNPVAMASTTVKPKASYTAGCTKAPLVSQITRYS 2023
Query: 1001 SPLRTRFIWAEIQRMCPFRLYCSMRSNIFLNSACSSLSLEGLPWSPQTTTRFTSSCSRLF 1180
SP TRFI EI PF L S+R IF SA SS S + SP TTTRFT+S S F
Sbjct: 2022 SPFLTRFI*EEIHLNWPFNL*VSIRPYIFFISAISSKSFDSFLLSPATTTRFTNSRSLAF 1843
Query: 1181 RPYSSTSPVKFFTRSRRAMEKMIGLFLSFRTNLCSVFSVWLYRSSSISG*REVSLLCDSV 1360
PY STSPV FFTRS AM K+IGL SF T++CS FS L RSSSISG*++V+ SV
Sbjct: 1842 LPYHSTSPVMFFTRSNLAMVKIIGLLRSFSTHMCSSFSTEL*RSSSISG*KDVNRRLVSV 1663
Query: 1361 YGK*MDRSAPGDTMLNLGSNTSMPWTTRYRPGIVNAMCDSYWPTVSLFPAISWKVLVMMK 1540
GK*M SAPGDTMLN GS TS+P TTR PG V A+ SY PT+SL PAISWKVLVMMK
Sbjct: 1662 *GK*MV*SAPGDTMLNFGSKTSIPCTTR*SPGRVKAV*LSYCPTLSLLPAISWKVLVMMK 1483
Query: 1541 SAWFIAIKSVVNWHEKW**SSNFFQ*RSELGYLVFSSAWAMVQ*VTPILWASKHATRFPS 1720
S WFIA +S VNW EK SSNFFQ* S+ GYLVFSSA AMV *VTP L A+K AT FPS
Sbjct: 1482 SVWFIAKRSAVNWQEK*YFSSNFFQ*ISDSGYLVFSSA*AMVH*VTPNLCANKEATMFPS 1303
Query: 1721 L*FPMIRSGLA*SSPAISCATSSV*VSRYGQTSNREIHLRTIPFSVLNGTRRIWQCSVPR 1900
L*FP IRSGL +S A S ATSSV*VSRYGQTSNREIHLRTIPFS L GTRR+WQCSVP
Sbjct: 1302 L*FPTIRSGLLCNSLASS*ATSSV*VSRYGQTSNREIHLRTIPFSSLKGTRRMWQCSVP* 1123
Query: 1901 TFSRRWPQVVPVASGSNLVTIRIFGVMSRPHCLILSSISFSIART*SKM*TTWPPVSG*P 2080
TFS RWPQVVP ASG V +R+ G++S+P L+ SIS S ART*SKM*TTWPPVSG P
Sbjct: 1122 TFSSRWPQVVPTASGRRRVIMRMRGMISKPCFLMRLSISLSKART*SKM*TTWPPVSGYP 943
Query: 2081 KTLA*AK*P*GERITTLNTMGIVPRNFSRVDGSGASRRSKRRWIVSCARLAQSPQPFSRP 2260
KT +*AK*P*GER+TTL T+GI+ R SRV GSGASRRSKR WI S R A SPQP S+P
Sbjct: 942 KTSS*AK*P*GERMTTLKTIGILSRKDSRVQGSGASRRSKRSWIDSRTRSAVSPQPLSKP 763
Query: 2261 SSWNLW*NSEYGVSACRVDRCSSAFLSAPCRALRLRILSFNIMVIPL*LWARRKLSKGYM 2440
SWNL NSEYGVS V R SSAFL R ILSFNI V PL*L + R G
Sbjct: 762 ISWNLCSNSEYGVSGSIVLRYSSAFLRTC*RESGF*ILSFNINVFPL*L*SLRNSKSGCK 583
Query: 2441 LSLSWKSFDDRCLLRNCTPLPMDFDREGRGKEALNGSKSSSRTKLLLGPSSTSCSLNCRY 2620
LSLSWKS +R LR CTP P+D R GRGK+A+NGSKS+S TK L STS SLN R
Sbjct: 582 LSLSWKSLAERWRLRKCTPFPIDLLRVGRGKDAVNGSKSNSSTKFPLALPSTSSSLNLRN 403
Query: 2621 SGXXXXXXXXXXXXXXXXXXXPGLGRMASATHGGMTIAS*AALRTSSKAPSLSFSRSASG 2800
S PGLGR ASATHGG TIAS L+TS APS+SF +SG
Sbjct: 402 SAGCKFSTTRACTFTLRYSQIPGLGRTASATHGGKTIASCVDLKTSPNAPSVSFCLLSSG 223
Query: 2801 IA 2806
I+
Sbjct: 222 IS 217
Score = 400 bits (868), Expect(4) = e-146
Identities = 180/407 (44%), Positives = 272/407 (66%)
Frame = +1 / -3
Query: 1210 VLHTVETSHGEDDWLVPVVQNELVLRVLGLAVQELLNLRVKGGQPLVRLGVREVDGQVRA 1389
VLHTV+ HGED+WLV V+Q+ VL +L AV++L + RV+G Q GVREV+G + +
Sbjct: 1813 VLHTVQPCHGEDNWLVAVLQHTYVLFILN*AVKKLFDFRVEGCQSAASFGVREVNGLISS 1634
Query: 1390 RRHDVELGVEHINAVDNAVQARHCERHV*LVLADGVLVSGDLLEGTGDDEVGMVHCNQVS 1569
R++VEL ++ IN V + V++ + V L+L++ +L S +LLEGT DDE+ +VHC ++S
Sbjct: 1633 WRYNVELWIKDINTVHDTVESWKSKGSVTLILSNLILASSNLLEGTSDDEICVVHCEKIS 1454
Query: 1570 GELAREVVVILKLLPVEVGVRVLSFLKRMGNGTVSNTHLVGEQTRNKVSVTVVSDDQVRI 1749
G+LA EV+++ + PV +G VLS L+ M NGT+S+T + +Q N VS+TVVS++Q+
Sbjct: 1453 GKLAGEVILLFEFFPVNIGFGVLSLLECMSNGTLSDT*FMCQQRGNNVSITVVSNNQIWF 1274
Query: 1750 GLKLSSNLMRHVISVSLQVRPDFKSRDPLANDSVFCSEWHAKDMAVLGAKDLLKALATGG 1929
L+L LM +++SVS + PDFKS DPLAN+S+ SE ++K++A+L + DL K LAT
Sbjct: 1273 ALQLFGKLMSNILSVSF*IWPDFKS*DPLANNSLLISEGNSKNVAMLSSIDLFKPLATSS 1094
Query: 1930 ASCIREQPGDNKDLRRDVKTTLLDPQQHFVLHSAHLIQDVDNLASGVRVTQDISLSEVTM 2109
+ CI E+ DN++ R D++ L+ +H L +LIQDV+NLAS +RV+QDI LS+VTM
Sbjct: 1093 SDCIGEKTSDNENARDDIQALFLNATEHLALQGTNLIQDVNNLASSIRVSQDIILSKVTM 914
Query: 2110 RGEDNDIEHHGDRSKELLQGGRIWGLQEVQEEVDSLLCTLSAITPTLLQTKFLEPVVKFR 2289
R +D++I+ H D KE L+G R+ L ++QE++D L L ++P LQT LEPV KF
Sbjct: 913 RRKDDNIKDHWDSIKERLKGARVRSL*KIQEKLDRLEDALCGVSPASLQTNLLEPVFKF* 734
Query: 2290 VWGISL*GGQVLLSLPQRTLQSTETADSVVQHHGHPLVVVGAKEVEQ 2430
VW + +L S PQ L+ +SV+QH LV V +++ ++
Sbjct: 733 VWCFRINCA*ILFSFPQNMLKRIGVLNSVIQHQCLSLVAVKSEKFQK 593
Score = 194 bits (419), Expect(5) = e-126
Identities = 108/258 (41%), Positives = 137/258 (53%)
Frame = -1 / +1
Query: 2782 EAQGWCF*GCPEGSSGGDCHPPMGCTCHPP*AWCLGVCEGQRQ*ARC*GAESS*VPAVQG 2603
EA WC E +G + MGCTC A LGV E + + C + *V +Q
Sbjct: 241 EAD*WCIW*SFEIHTGSNSFATMGCTCCSSKARYLGVSESECACSCCRKFATC*VSQIQR 420
Query: 2602 TACGRRPQQQLCS*AGL*AIQCLLPPSFSVKVHWQWRAVPQQAPVIKALP*QGEHVPLAQ 2423
C + +LC+* GL* I C+ P S+S +V+W+W A+P LP*QGE
Sbjct: 421 RTC*WKC*WKLCA*VGL*TIYCIFPSSYSQQVNWKWCALP*SPSFC*TLP*QGEFTSTFG 600
Query: 2422 LPSRPQLQGDDHDVERQNPQSQCSARCAEEG*GAPVHPTS*YPIL*ISPQVPGTWSGEGL 2243
+ QLQG D DVE QN + + S+ C+EE * H S* IL I QVPG W GE L
Sbjct: 601 ISQTSQLQGKDIDVE*QNSKPRFSSTCSEES*RVSKHN*S*NTILRI*TQVPGDWFGERL 780
Query: 2242 G*LR*ACTGDYXXXXXXXXXPRSVHPGEVPWNDPHGVQCRYPLPSWLLRSS*CLGLP*HR 2063
G R A Y +HP P +P+G+ C +P SWLL S *CLG+P*+
Sbjct: 781 GRHRRARPRVYPTSLGSSRGS*PLHP*VFP**NPNGL*CCHPFSSWLLCSR*CLGIP*YW 960
Query: 2062 RPGCLHLGSSARYGERNA 2009
RPGCLHLGSS+ GER+A
Sbjct: 961 RPGCLHLGSSSCLGERDA 1014
Score = 159 bits (342), Expect(5) = e-126
Identities = 92/224 (41%), Positives = 123/224 (54%)
Frame = -1 / +1
Query: 1555 NEPCRLHHHQYLPRDRRKQGHRRPVRVTHGVHNAWPVPRCPRH*CVRPQVQHRVSWRGPV 1376
NEP R HHH YLPRD KQG V +H ++++ +P R+*C+ +VQH +S
Sbjct: 1468 NEPHRFHHH*YLPRDCWKQG*GWTV*ESHCLYSSRTLPCRARY*CL*SKVQHCISRS*SD 1647
Query: 1375 HLLPVHRVAQEADLPSPGD*GAPVQPNREHGAQVRSERQEQANHLLHGSSRPCEELDWAG 1196
HLLP+HR D+ P + A +Q + E A + +E +QAN+L HG PCEE
Sbjct: 1648 HLLPLHRN*PPIDILLP*NRRASLQLS*E*RAHMCAEGPQQANYLHHGKVGPCEEHYRTC 1827
Query: 1195 GAVRPEQAAAGAGEPRGRLRRPWQPFQGQGGAGRVQEDV*PHRAVQPERAHPLDLRPDEP 1016
G VR E A+ GEP RR + +G G +EDV P+ +Q E +D DEP
Sbjct: 1828 GVVRQECQAS*VGEPCCCGRRQEEGVKGLGRDS*DEEDVWPN*DLQVEWPIQMDFLSDEP 2007
Query: 1015 RPQRRAVPLHLRHQGRLRAACFLRGFRADGG*GHDLRPAHVCHS 884
+RRAVP L HQG RA C +R FR D *GH A++C+S
Sbjct: 2008 CQKRRAVPCDL*HQGSFRATCRVRSFRFDSC*GHGYWIANICNS 2139
Score = 110 bits (235), Expect(5) = e-126
Identities = 53/120 (44%), Positives = 75/120 (62%)
Frame = -1 / +1
Query: 1981 HHAEDPYCHQVAP*CNWHHLWPAP*EGPWHRALPYPSRAIQNRKRNRSQVDLAI*SLAVP 1802
+H + H +P C+ ++LWP *+G W+ ALP+ S ++Q + N SQVDL I*SLA+
Sbjct: 1042 YHPSHSHYHSSSPRCSRNYLWPTA*KGLWN*ALPHSSSSLQR*EGNCSQVDLTI*SLAIS 1221
Query: 1801 GDLH**RGA*DCWRASGQS*PDHRKLQ*RKPCCVFARPQDGCYSLYHCPCA*EN*VP*LR 1622
+LH* +* C R + Q+ D KLQ* K CC+F C+S+YHC C E+*VP +R
Sbjct: 1222 RNLH*GCCS*ACQRVAKQTRSDCWKLQ*WKHCCLFVGT*IRCHSVYHCSCTRED*VPRIR 1401
Score = 103 bits (219), Expect(5) = 5e-044
Identities = 59/133 (44%), Positives = 65/133 (48%)
Frame = -3 / +3
Query: 2408 TTTRG*P*C*TTESAVSVLCKVR*GRLRSTCPPYKLIPHTLNFTTGSRNLVWRRVGVIAL 2229
T TR *C* TE +L + *G+L+S L HT N TGSR LVWR G
Sbjct: 615 TATRERH*C*MTEFKTPILFNMF*GKLKSI*AQLILKHHTQNLNTGSRRLVWREAGETPQ 794
Query: 2228 SVHRRLXXXXXXXWRPQIRPPWRSSLERSPWCSMSLSSPLMVTSLKLMSWVTLTPEARLS 2049
S L R P SL S W M S LMVT LK+MSW TL EARL
Sbjct: 795 SASSSLSNFSWIF*RLLTLAPLSLSLIESQWSLMLSSFLLMVTLLKMMSWDTLILEARLF 974
Query: 2048 TSWIKCALWRTKC 2010
TSWIK WR +C
Sbjct: 975 TSWIKFVPWRARC 1013
Score = 98.7 bits (209), Expect(7) = 2e-078
Identities = 56/134 (41%), Positives = 69/134 (51%)
Frame = +3 / -2
Query: 1188 TAPPAQSSSSHGRDEPWRR*LACSCRSERTCAPCSRFGCTGAPQSPGEGRSASCATRCTG 1367
T P SSHG PW R*LAC S CA S+ C A + G S RC G
Sbjct: 1835 TTPQVL*CSSHGPTLPW*R*LACCGPSAHICALHSQLSCKEALRFQGRRMSIGG*FRCKG 1656
Query: 1368 SRWTGPRQETRC*TWGRTHQCRGQRGTGQAL*TPCVTRTGRRCPCFRRSLGRYW***SRH 1547
S+W+ E +C*T + HQ R + G * C + T + PCF++SLGRY ***+
Sbjct: 1655 SKWSDQLLEIQC*TLDQRHQYRARHGRVLEE*RQCDSHTVQPYPCFQQSLGRY****NLC 1476
Query: 1548 GSLQSSQW*TGTRS 1589
GSL+ Q * G RS
Sbjct: 1475 GSLRKDQR*IGRRS 1434
Score = 87.2 bits (184), Expect(7) = 2e-078
Identities = 44/101 (43%), Positives = 54/101 (53%)
Frame = +3 / -2
Query: 1644 SQAHGQWYSE*HPSCGRANTQQGFRHCSFR*SGQDWPEALQQSHAPRHQCKSPGTARLQI 1823
S+ H QWY+E*H QQ F HCSF+ S L Q+H QCK ARLQI
Sbjct: 1379 SRVHEQWYTE*HLIYVPTKRQQCFHHCSFQQSDLVCFATLWQAHEQHPQCKFLDMARLQI 1200
Query: 1824 ARSTCERFRFLF*MAREGYGSARCQGPSQGAGHRWCQLHQG 1946
RSTCE+F * E G+A+ P Q GH+ +LH+G
Sbjct: 1199 VRSTCEQFPSHL*RELEECGNAQFHRPFQAVGHK*FRLHRG 1077
Score = 83.1 bits (175), Expect(4) = e-146
Identities = 38/104 (36%), Positives = 57/104 (54%)
Frame = +1 / -3
Query: 2491 HAIANGL*QRRTGEGGIEWLKVQLKNKVVVGAFFHKLFLELQVLRNSQLLNSELTDVDPH 2670
H I+N L + RT E +W KVQLK+KV + +K F E + L Q+ +++ +
Sbjct: 532 HTISN*LVESRTRERCSKWFKVQLKHKVSISTSINKFFFEFEKLSRLQIFDNKSMHIHSQ 353
Query: 2671 ILPDTRPRADGKCNPWGDDNRLLSCPQDILKSTILELLALSLRD 2802
ILP+T P + KCNPW + L Q+ K TI +LL+ R+
Sbjct: 352 ILPNTWPWTNSKCNPWWQNYCFLCGSQNFTKCTISQLLSTLFRN 221
Score = 73.9 bits (155), Expect(7) = 2e-078
Identities = 29/45 (64%), Positives = 36/45 (80%)
Frame = +3 / -2
Query: 2034 LDPRCRQPGLRCQGNPRH*LERSNHEGRG*RH*TPWGSFQGTSPG 2168
LDPRC+QPGL+ QG PRH LE+SNHE +G*+H* P G +QG + G
Sbjct: 989 LDPRCKQPGLQYQGIPRHHLEQSNHEEKG*QH*RPLGFYQGKTQG 855
Score = 64.3 bits (134), Expect(2) = 0.0
Identities = 24/56 (42%), Positives = 44/56 (78%)
Frame = -2 / +2
Query: 2949 LSRLHSVRERIGDSLSAHPNELVAVFTRLKNLGKGMLQPHQIIAEYNNAIPEAERE 2782
L+R+HS++ER+ ++L+A+ NE++A+ +RL+ GKG+LQ HQ+IAE+ E+ ++
Sbjct: 77 LTRVHSLKERLDETLTANRNEILALLSRLEAKGKGILQHHQVIAEFEEIPEESRQK 244
Score = 61.1 bits (127), Expect(4) = e-146
Identities = 26/62 (41%), Positives = 37/62 (59%)
Frame = +1 / -3
Query: 562 HGARHGAVLERVQHLQVPPGLAPLQVGHVLPEPVHAGEGHQPLRVQLPGVLLLDTLEPAL 741
H AVLE ++HL++ L ++V H+LPE + E +PLRV P +LL +TLE L
Sbjct: 2461 HRLSQFAVLESIEHLKIAAALKTVEVRHMLPEAIDTSECKKPLRVNCPCILLFNTLETTL 2282
Query: 742 GD 747
D
Sbjct: 2281 RD 2276
Score = 47.8 bits (98), Expect(7) = 2e-078
Identities = 23/44 (52%), Positives = 25/44 (56%)
Frame = +3 / -2
Query: 2442 SPCHGRALMTGAC*GTARHCQWTLTEKDGGRRH*MAQSPAQEQS 2573
SPCHGR *G+A H Q T E D G+ *M QSP Q QS
Sbjct: 581 SPCHGRV*QKDGD*GSAHHFQLTC*E*DEGKMQ*MVQSPTQAQS 450
Score = 46.0 bits (94), Expect(4) = e-146
Identities = 20/62 (32%), Positives = 29/62 (46%)
Frame = +1 / -3
Query: 871 RTAVGCGKRGQAAGHGLNHRQPESLVESRLHEXXXXXXXXXXXXXXXXXXHLGGDPADVP 1050
R + K Q++ HGLN+ Q ES V RLHE HL G+P+++
Sbjct: 2152 RATIKSCKCWQSSSHGLNNCQTESFVHGRLHESSLGVTNHTVQLSVSDTVHLRGNPSELA 1973
Query: 1051 VQ 1056
+Q
Sbjct: 1972 IQ 1967
Score = 44.6 bits (91), Expect(5) = 5e-044
Identities = 24/46 (52%), Positives = 28/46 (60%)
Frame = -3 / +3
Query: 1946 SLMQLAPPVASALRRSLAPSTAISFACHSEQKTESFASGSRDLKSG 1809
S MQ VA+ L+RS+ S A F SE + E FASGS DLKSG
Sbjct: 1077 SPMQSELLVANGLKRSMELSIATFFEFPSEMRRELFASGSHDLKSG 1214
Score = 42.8 bits (87), Expect(5) = 5e-044
Identities = 26/61 (42%), Positives = 33/61 (54%)
Frame = -3 / +3
Query: 1679 VLLTVPLPMRLRKLSTLTPTSTGRSLRITTTSRASSPLT*LQ*TMXXXXXXXXSKRSPET 1500
V L+VPL M R+LST P TG++ + + TS A+ PL Q*T SKR E
Sbjct: 1344 VSLSVPLLMHSRRLSTPNPIFTGKNSKRSITSPANLPLIFSQ*TTQISSSLVPSKRLLEA 1523
Query: 1499 R 1497
R
Sbjct: 1524 R 1526
Score = 40.5 bits (82), Expect(5) = 5e-044
Identities = 24/61 (39%), Positives = 30/61 (49%)
Frame = -3 / +3
Query: 2624 LSTCSSRNSLWKKAPTTTLFLSWTLSHSMPPSPVLLCQSPLAMAXXXXXXXXXXXXSMTR 2445
LS +S+ +L + TL LSWTL+H + S VLL S L M SMTR
Sbjct: 399 LSFSNSKKNLLMEVLMETLCLSWTLNHLLHLSLVLLSTSQLEMVCTSLIAIFLLNSSMTR 578
Query: 2444 R 2442
R
Sbjct: 579 R 581
Score = 37.7 bits (76), Expect(5) = e-126
Identities = 21/59 (35%), Positives = 33/59 (55%)
Frame = -1 / +1
Query: 757 LEQDLPGRAPAYRGEVHLEAVLGEADDPHRRVRVLEVRVQPGEARDPAVPGDAVRAQVP 581
L QDL +P Y EV+++ +L EA H + +LE V P + +P + D + +QVP
Sbjct: 2266 LGQDLSRWSPTY*REVYMDNLLSEASYTHWCLWLLEACV*PRPS*EPPLS*DVLCSQVP 2442
Score = 37.3 bits (75), Expect(5) = 5e-044
Identities = 22/55 (40%), Positives = 24/55 (43%)
Frame = -3 / +3
Query: 725 VSRRSTPGSCTRRG**PSPACTGSGSTCPTWRGARPGGTWRCCTRSSTAPWRAPC 561
V +RS G T RG S SGS C T R RC SSTA W + C
Sbjct: 2298 VLKRSIHGQFTLRGFLHSLVSMASGSMCLTSTVLRAAAILRCSMLSSTANWLSLC 2462
Score = 35.9 bits (72), Expect(7) = 2e-078
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = +3 / -2
Query: 2328 QPSSAHLAEH*DCGFCRSTSWSSPCSC 2408
Q SS H+ E+ FC STS S PCSC
Sbjct: 695 QLSSEHVEENRGFEFCHSTSMSFPCSC 615
Score = 31.3 bits (62), Expect(7) = 2e-078
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = +3 / -2
Query: 2241 PNPSPDQVPGTCGEIQSMGYQ 2303
P+ SP+Q PGTC +I SM +Q
Sbjct: 782 PSLSPNQSPGTCVQILSMVFQ 720
Score = 25.8 bits (50), Expect(3) = 0.0
Identities = 12/25 (48%), Positives = 14/25 (56%)
Frame = +2 / -1
Query: 2816 SAMIWWGCSIPFPRFFSLVKTATSS 2890
SA+ W C IPFP SL + A S
Sbjct: 210 SAIT*WCCKIPFPFASSLERRAKIS 136
Score = 25.4 bits (49), Expect(7) = 2e-078
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = +3 / -2
Query: 2619 TQELSAPQQRAH*R*PSHTPRHQA*GGWQVQPM 2717
TQ+++ +Q+ H S TP++ A QVQPM
Sbjct: 404 TQQVANFRQQEHAHSLSDTPKYLALDEQQVQPM 306
Score = 23.5 bits (45), Expect(5) = e-126
Identities = 7/15 (46%), Positives = 10/15 (66%)
Frame = -1 / +1
Query: 865 DHRARRVWLPHRPLP 821
DH ++W+PH LP
Sbjct: 2158 DHCPWQIWIPH*SLP 2202
Score = 22.6 bits (43), Expect(3) = 0.0
Identities = 10/26 (38%), Positives = 13/26 (50%)
Frame = +3 / -2
Query: 561 ARCSPWCGT*ARTASPGTAGSRASPG 638
A+ P CGT* S + GS+ G
Sbjct: 2462 AQTQPICGT*EHRTSQDSGGSQDGRG 2385
>gb|DQ122788.1| Medicago sativa clone B11 sucrose synthase mRNA, partial cds
Length = 536
Score = 236 bits (510), Expect(2) = 2e-081
Identities = 92/122 (75%), Positives = 105/122 (86%)
Frame = -2 / +2
Query: 1119 SKDKEEQAEFKKMFDLIEQYNLNGHIRWISAQMNRVRNGELYRYICDTKGAFVQPAFYEA 940
S D+EE AE +KM DL++QYNLNG RW++AQ NR RNGELYRYI DTKGAFVQPAFYEA
Sbjct: 167 SSDREEIAEIEKMHDLMKQYNLNGEFRWLTAQTNRARNGELYRYIADTKGAFVQPAFYEA 346
Query: 939 FGLTVVEAMTCGLPTFATAYGGPAEIIVHGVSGYHIDPYQGDKASALLVDFFDKCQAEPS 760
FGLTVVEAMTCGLPTFAT +GGPAEII HGVSG+HIDPY DKAS LL++FF K + +P+
Sbjct: 347 FGLTVVEAMTCGLPTFATCHGGPAEIIEHGVSGFHIDPYHPDKASELLLEFFQKSKEDPN 526
Query: 759 HW 754
HW
Sbjct: 527 HW 532
Score = 169 bits (364), Expect(2) = 6e-051
Identities = 82/122 (67%), Positives = 92/122 (75%)
Frame = +2 / -3
Query: 755 QWLGSAWHLSKKSTSRADALSPW*GSMW*PDTPCTMISAGPP*AVANVGRPQVMASTTVS 934
QW GS+ K S++ ++ALS *GS+ PDTPC+MISAGPP VANVG PQVMASTTVS
Sbjct: 531 QWFGSSLLFWKNSSNSSEALSG**GSI*NPDTPCSMISAGPPWQVANVGSPQVMASTTVS 352
Query: 935 PKAS*KAGCTKAPLVSQM*RYSSPLRTRFIWAEIQRMCPFRLYCSMRSNIFLNSACSSLS 1114
PKAS*KAG TKAP VS M*RYSSPLR F+ A R PF+LYC MRS IF SA SSLS
Sbjct: 351 PKAS*KAG*TKAPFVSAM*RYSSPLRALFV*AVSHRNSPFKLYCFMRSCIFSISAISSLS 172
Query: 1115 LE 1120
L+
Sbjct: 171 LD 166
Score = 83.1 bits (175), Expect(2) = 2e-081
Identities = 35/45 (77%), Positives = 40/45 (88%)
Frame = -2 / +2
Query: 1269 LNDRNKPIIFSMARLDRVKNLTGLVELYGRNKRLQELVNLVVVCG 1135
L DR+KPIIFSMARLDRVKN+TGLVE Y +N +L+ELVNLVVV G
Sbjct: 14 LADRSKPIIFSMARLDRVKNITGLVESYAKNSKLRELVNLVVVAG 148
Score = 48.3 bits (99), Expect(2) = 6e-051
Identities = 25/45 (55%), Positives = 27/45 (60%)
Frame = +2 / -3
Query: 1136 PQTTTRFTSSCSRLFRPYSSTSPVKFFTRSRRAMEKMIGLFLSFR 1270
P TTTR TSS + LF ST PV F T S AMEK+IG S R
Sbjct: 147 PATTTRLTSSLNLLFLA*LSTKPVMFLTLSSLAMEKIIGFDRSAR 13
>gb|CO514282.1|CO514282 s13dSG76F0500043_157226 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 515
Score = 208 bits (449), Expect(2) = 8e-075
Identities = 83/103 (80%), Positives = 90/103 (87%)
Frame = -2 / +1
Query: 1119 SKDKEEQAEFKKMFDLIEQYNLNGHIRWISAQMNRVRNGELYRYICDTKGAFVQPAFYEA 940
S D+EE AE +KM DL++QYNLNG RWI+AQ NR RNGELYRYI DTKGAFVQPAFYEA
Sbjct: 205 SSDREEIAEIEKMHDLMKQYNLNGEFRWITAQTNRARNGELYRYIADTKGAFVQPAFYEA 384
Query: 939 FGLTVVEAMTCGLPTFATAYGGPAEIIVHGVSGYHIDPYQGDK 811
FGLTVVEAMTCGLPTFAT +GGPAEII HGVSG+HIDPY DK
Sbjct: 385 FGLTVVEAMTCGLPTFATCHGGPAEIIEHGVSGFHIDPYHPDK 513
Score = 157 bits (338), Expect(2) = 3e-046
Identities = 77/104 (74%), Positives = 82/104 (78%)
Frame = +2 / -1
Query: 809 ALSPW*GSMW*PDTPCTMISAGPP*AVANVGRPQVMASTTVSPKAS*KAGCTKAPLVSQM 988
ALS *GS+ PDTPC+MISAGPP VANVG PQVMASTTVSPKAS*KAG TKAP VS M
Sbjct: 515 ALSG**GSI*NPDTPCSMISAGPPWQVANVGSPQVMASTTVSPKAS*KAG*TKAPFVSAM 336
Query: 989 *RYSSPLRTRFIWAEIQRMCPFRLYCSMRSNIFLNSACSSLSLE 1120
*RYSSPLR F+ A I R PF+LYC MRS IF SA SSLSL+
Sbjct: 335 *RYSSPLRALFV*AVIHRNSPFKLYCFMRSCIFSISAISSLSLD 204
Score = 89.0 bits (188), Expect(2) = 8e-075
Identities = 39/61 (63%), Positives = 47/61 (77%)
Frame = -2 / +1
Query: 1317 EELLYSQTENTEHKFVLNDRNKPIIFSMARLDRVKNLTGLVELYGRNKRLQELVNLVVVC 1138
E+LLY + E L DR+KPIIFSMARLDRVKN+TGLVE Y +N +L+ELVNL+VV
Sbjct: 4 EKLLYDTEQTDECIGSLADRSKPIIFSMARLDRVKNITGLVESYAKNSKLRELVNLIVVA 183
Query: 1137 G 1135
G
Sbjct: 184 G 186
Score = 44.6 bits (91), Expect(2) = 3e-046
Identities = 22/39 (56%), Positives = 24/39 (61%)
Frame = +2 / -1
Query: 1136 PQTTTRFTSSCSRLFRPYSSTSPVKFFTRSRRAMEKMIG 1252
P TT R TSS + LF ST PV F T S AMEK+IG
Sbjct: 185 PATTIRLTSSLNLLFLA*LSTKPVMFLTLSSLAMEKIIG 69
>gb|CO515699.1|CO515699 s13dSG60G0600052_419547 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 449
Score = 273 bits (591), Expect = 7e-074
Identities = 115/148 (77%), Positives = 124/148 (83%)
Frame = -2 / +3
Query: 1344 RLTSLHPEIEELLYSQTENTEHKFVLNDRNKPIIFSMARLDRVKNLTGLVELYGRNKRLQ 1165
RL S HPEIEELLYS EN EH VL DR+KPIIF+MARLDRVKN+TGLVE YG+N RL+
Sbjct: 6 RLKSFHPEIEELLYSSVENEEHICVLKDRSKPIIFTMARLDRVKNITGLVEWYGKNARLR 185
Query: 1164 ELVNLVVVCGDHGNPSKDKEEQAEFKKMFDLIEQYNLNGHIRWISAQMNRVRNGELYRYI 985
ELVNLVVV GD SKD EE+AE KKM+ LIE Y LNG RWIS+QMNR+RNGELYR I
Sbjct: 186 ELVNLVVVAGDRRKESKDLEEKAEMKKMYGLIETYKLNGQFRWISSQMNRIRNGELYRVI 365
Query: 984 CDTKGAFVQPAFYEAFGLTVVEAMTCGL 901
CDTKGAFVQPA YEAFGLTVVEAMTCGL
Sbjct: 366 CDTKGAFVQPAIYEAFGLTVVEAMTCGL 449
Score = 182 bits (393), Expect = 2e-046
Identities = 92/147 (62%), Positives = 101/147 (68%)
Frame = +2 / -2
Query: 905 PQVMASTTVSPKAS*KAGCTKAPLVSQM*RYSSPLRTRFIWAEIQRMCPFRLYCSMRSNI 1084
PQV+ASTTV PKAS* AGCTKAP VSQM R SSP R RFI EI PF L S+R I
Sbjct: 445 PQVIASTTVKPKAS*IAGCTKAPFVSQMTR*SSPFRIRFICEEIHLNWPFNL*VSIRPYI 266
Query: 1085 FLNSACSSLSLEGLPWSPQTTTRFTSSCSRLFRPYSSTSPVKFFTRSRRAMEKMIGLFLS 1264
F SA SS SL+ SP TTTRFTSS R F PY STSPV FFTRS A+ K+IGL S
Sbjct: 265 FFISAFSSKSLDSFLLSPATTTRFTSSLRRAFLPYHSTSPVMFFTRSSLAIVKIIGLLRS 86
Query: 1265 FRTNLCSVFSVWLYRSSSISG*REVSL 1345
F T++CS FS L RSSSISG + +L
Sbjct: 85 FNTHMCSSFSTEL*RSSSISGWNDFNL 5
>gb|CO517054.1|CO517054 s13dSG31G0500037_468654 Glandular trichomes Medicago sativa cDNA,
mRNA sequence
Length = 374
Score = 243 bits (525), Expect = 9e-065
Identities = 102/123 (82%), Positives = 107/123 (86%)
Frame = -2 / +1
Query: 2232 AKRAQETIHLLLDLLEAPDPSTLEKFLGTIPMVFNVVILSPHGYFAQANVLGYPDTGGQV 2053
A+R E I LLLDLLEAPDP TLEKFLG IPM FN VILSPHGYFAQ N LGYPDTGGQV
Sbjct: 4 AERVLEMIQLLLDLLEAPDPFTLEKFLGRIPMAFNAVILSPHGYFAQDNALGYPDTGGQV 183
Query: 2052 VYILDQVRAMENEMLLRIKQCGLDITPKILIVTRLLPDATGTTCGQRLEKVLGTEHCHIL 1873
VYILDQVRA+ENEML RIKQ GLDI P+ILI+TRLLPDA GTTCG+RLEKV TEHCHIL
Sbjct: 184 VYILDQVRALENEMLRRIKQQGLDIKPRILIITRLLPDAVGTTCGERLEKVYDTEHCHIL 363
Query: 1872 RVP 1864
RVP
Sbjct: 364 RVP 372
Score = 183 bits (395), Expect = 8e-047
Identities = 84/124 (67%), Positives = 95/124 (76%)
Frame = +2 / -1
Query: 1862 NGTRRIWQCSVPRTFSRRWPQVVPVASGSNLVTIRIFGVMSRPHCLILSSISFSIART*S 2041
NGTRR+WQCSV TFSRR PQVVP ASG V +R+ G++S+P CLI SISFS ART S
Sbjct: 374 NGTRRMWQCSVSYTFSRRSPQVVPTASGRRRVIMRMRGLISKPCCLIRLSISFSKARTWS 195
Query: 2042 KM*TTWPPVSG*PKTLA*AK*P*GERITTLNTMGIVPRNFSRVDGSGASRRSKRRWIVSC 2221
KM TT PPVSG PK L+ AK*P GER+T L +GI+PRNFSRV GSGASRRS R WI+S
Sbjct: 194 KMYTTCPPVSGYPKALSWAK*PCGERMTALKAIGILPRNFSRVKGSGASRRSNRSWIISR 15
Query: 2222 ARLA 2233
R A
Sbjct: 14 TRSA 3
Score = 128 bits (274), Expect = 4e-030
Identities = 53/122 (43%), Positives = 83/122 (68%)
Frame = +1 / -3
Query: 1864 WHAKDMAVLGAKDLLKALATGGASCIREQPGDNKDLRRDVKTTLLDPQQHFVLHSAHLIQ 2043
W++K++A+ G LK T + CIRE+ GDN++ R D++ LLD +H VL ++L+Q
Sbjct: 372 WNSKNVAMFGIVYFLKTFTTSSSHCIREKTGDNENARLDIQALLLDTSEHLVLQGSNLVQ 193
Query: 2044 DVDNLASGVRVTQDISLSEVTMRGEDNDIEHHGDRSKELLQGGRIWGLQEVQEEVDSLLC 2223
+V NL++G+RV+Q I L EVTM +D+ I+ H D SKE L+G RI L+++Q+++D
Sbjct: 192 NVHNLSTGIRVSQGIILGEVTMWRKDDSIKGHRDSSKEFLEGERIRCLEKIQQKLDHFKD 13
Query: 2224 TL 2229
TL
Sbjct: 12 TL 7
Score = 112 bits (239), Expect = 3e-025
Identities = 46/105 (43%), Positives = 66/105 (62%)
Frame = -1 / +3
Query: 2176 SVHPGEVPWNDPHGVQCRYPLPSWLLRSS*CLGLP*HRRPGCLHLGSSARYGERNAAEDQ 1997
S HP E+PW +P+G+ C +P +WLLR *CLG+P*+R GC+H G S+ GER+A Q
Sbjct: 60 SFHPREIPWKNPYGL*CCHPFSTWLLRPR*CLGIP*YRWTGCVHFGPSSSLGERDAQTYQ 239
Query: 1996 AVWS*HHAEDPYCHQVAP*CNWHHLWPAP*EGPWHRALPYPSRAI 1862
A + A + H +P*C+ ++LW *E +R LP+ S +I
Sbjct: 240 ATRLGYQASHSHYHPSSP*CSGNYLW*TS*ESIRYRTLPHSSSSI 374
>gb|DQ122842.1| Medicago sativa clone MF8 sucrose synthase mRNA, partial cds
Length = 652
Score = 180 bits (387), Expect = 1e-045
Identities = 72/102 (70%), Positives = 88/102 (86%)
Frame = -2 / +2
Query: 2802 IPEAEREKLKDGAFEDVLRAAQEAIVIPPWVALAIRPRPGVWEYVRVNVSELAVEELRVP 2623
IPE +R+KL +GAF +VLR+ QEA+V+PP+VALA+RPRPGVWEY+RV+V L V+ELR
Sbjct: 347 IPEEKRQKLVNGAFGEVLRSTQEAVVLPPFVALAVRPRPGVWEYLRVDVHSLVVDELRAA 526
Query: 2622 EYLQFKEQLVEEGPNNNFVLELDFEPFNASFPRPSLSKSIGN 2497
EYL+FKE+LVE N NFVLELDFEPFNA FPRP+L+KSIGN
Sbjct: 527 EYLKFKEELVEGSSNGNFVLELDFEPFNAPFPRPTLNKSIGN 652
Score = 104 bits (221), Expect(2) = 2e-024
Identities = 53/102 (51%), Positives = 59/102 (57%)
Frame = +2 / -2
Query: 2501 PMDFDREGRGKEALNGSKSSSRTKLLLGPSSTSCSLNCRYSGXXXXXXXXXXXXXXXXXX 2680
P+D R GRGK ALNGSKS+S TKL L STS SLN RYS
Sbjct: 648 PIDLFRVGRGKGALNGSKSNSSTKLPLELPSTSSSLNLRYSAARSSSTTRLCTSTLRYSQ 469
Query: 2681 XPGLGRMASATHGGMTIAS*AALRTSSKAPSLSFSRSASGIA 2806
PGLGR A AT+GG T AS* L+TS KAP SF +SGI+
Sbjct: 468 TPGLGRTARATNGGNTTAS*VDLKTSPKAPLTSFCLFSSGIS 343
Score = 24.4 bits (47), Expect(2) = 2e-024
Identities = 12/26 (46%), Positives = 14/26 (53%)
Frame = +2 / -2
Query: 2816 SAMIWWGCSIPFPRFFSLVKTATSSL 2893
SA+ W C IPFP L K A S+
Sbjct: 336 SAIT*WCCRIPFPFASILDKRAKISI 259
Database: MedicagoSativa_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:23 PM
Number of letters in database: 3,745,706
Number of sequences in database: 7669
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 27,087,189
Number of Sequences: 7669
Number of extensions: 403844
Number of successful extensions: 2514
Number of sequences better than 1.0e-020: 6
length of database: 1,248,568
effective HSP length: 46
effective length of database: 895,794
effective search space used: 913709880
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)