TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCT19a08.yg.2.1
(645 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA017100.1|CA017100 HV14M18u HV Hordeum vulgare subsp. v... 258 3e-068
gb|CA007888.1|CA007888 HU09E19r HU Hordeum vulgare subsp. v... 200 4e-058
gb|CA001848.1|CA001848 HS05M10r HS Hordeum vulgare subsp. v... 180 8e-045
gb|BM373115.2|BM373115 EBma04_SQ003_C15_R maternal, 10 DPA,... 151 1e-043
gb|CB876810.1|CB876810 HP02H19T HP Hordeum vulgare subsp. v... 136 2e-039
gb|CB879498.1|CB879498 HP02H19w HP Hordeum vulgare subsp. v... 136 2e-039
gb|BI947034.1|BI947034 HVSMEl0003F09f Hordeum vulgare spike... 150 5e-036
gb|BF624819.3|BF624819 HVSMEa0017N07f Hordeum vulgare seedl... 140 6e-033
gb|BF255465.2|BF255465 HVSMEf0006O24f Hordeum vulgare seedl... 140 6e-033
gb|BF260744.3|BF260744 HVSMEf0022N07f Hordeum vulgare seedl... 140 6e-033
gb|BQ758997.1|BQ758997 EBma07_SQ003_P08_R maternal, 21 DPA,... 138 4e-032
gb|BU969789.1|BU969789 HB12J02r BC Hordeum vulgare subsp. v... 113 1e-024
gb|BG301296.1|BG301296 HVSMEb0020I18f Hordeum vulgare seedl... 79 5e-024
gb|BG299925.1|BG299925 HVSMEa0022I12f Hordeum vulgare seedl... 79 5e-024
gb|BF627500.2|BF627500 HVSMEb0005A10f Hordeum vulgare seedl... 77 1e-023
gb|BF630433.1|BF630433 HVSMEb0009F21f Hordeum vulgare seedl... 74 1e-022
gb|BJ466753.1|BJ466753 BJ466753 K. Sato unpublished cDNA li... 73 2e-022
gb|BU970614.1|BU970614 HB15C18r BC Hordeum vulgare subsp. v... 103 8e-022
gb|BF621296.2|BF621296 HVSMEa0006G08f Hordeum vulgare seedl... 73 1e-021
gb|BU970588.1|BU970588 HB15B14r BC Hordeum vulgare subsp. v... 101 3e-021
gb|BU968318.1|BU968318 HB07C12r BC Hordeum vulgare subsp. v... 74 5e-021
gb|BQ472572.1|BQ472572 HB09C12T BC Hordeum vulgare subsp. v... 74 5e-021
>gb|CA017100.1|CA017100 HV14M18u HV Hordeum vulgare subsp. vulgare cDNA clone HV14M18
3-PRIME, mRNA sequence
Length = 576
Score = 258 bits (557), Expect = 3e-068
Identities = 100/122 (81%), Positives = 110/122 (90%)
Frame = +2 / +1
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
INNGL SC+P EVG+LREVTVFDVSFN+L GPLP QVAGM+KVEQ D+AHN LSG VPEA
Sbjct: 199 INNGLTSCLPAEVGMLREVTVFDVSFNALAGPLPPQVAGMQKVEQFDIAHNLLSGTVPEA 378
Query: 191 ICALPRLKNLTISYNFFTGEPPSCARVVPSDGDRRNCLPNRPTQRTPQQCAAFYSQPPVD 370
+C LPRLKN T SYNFFTGEPPSCARVVP+DGDRRNCLP+RP QR PQQCAAFY++PPVD
Sbjct: 379 VCDLPRLKNFTFSYNFFTGEPPSCARVVPADGDRRNCLPDRPAQRMPQQCAAFYARPPVD 558
Query: 371 CA 376
CA
Sbjct: 559 CA 564
Score = 206 bits (444), Expect = 1e-052
Identities = 91/122 (74%), Positives = 95/122 (77%)
Frame = -1 / -2
Query: 375 AQSTGGCE*NAAHCCGVRCVGRLGRQFLLSPSEGTTRAQDGGSPVKKL*EMVRFLSRGRA 196
AQSTGG * AAHCCG+R GR GRQF SPS GTTRAQDGGSPVKKL*E V+FLSRGR+
Sbjct: 563 AQSTGGRA*KAAHCCGMRWAGRSGRQFRRSPSAGTTRAQDGGSPVKKL*EKVKFLSRGRS 384
Query: 195 QMASGTAPDRRLCATSSCSTLRIPATCCGSGPTSELKLTSKTVTSRSKPTSGGTQESRPL 16
ASGT PD RLCA S+CST IPATC GSGP LKLTS TVTSRS PTS G E RPL
Sbjct: 383 HTASGTVPDSRLCAMSNCSTFCIPATCGGSGPARALKLTSNTVTSRSIPTSAGRHEVRPL 204
Query: 15 LM 10
LM
Sbjct: 203 LM 198
Score = 172 bits (371), Expect = 1e-042
Identities = 79/111 (71%), Positives = 85/111 (76%)
Frame = -3 / -1
Query: 370 VDGRLRVERGALLRRALCGAVGQAVPPVTVRRHDARAGRRLAGEEVVGDGQVLEPREGAD 191
VDG +E GALLR AL GAVG+AVP V V RHDARAG RLAGEEVVG+G+VLEP E A
Sbjct: 558 VDGWAGIESGALLRHALGGAVGEAVPAVAVGRHDARAGWRLAGEEVVGEGEVLEPWEVAH 379
Query: 190 GLRDGPGQAVVRDVQLLHLAHPGHLLRQRANERVEADVEDGHLPEQADLRR 38
GLR GP Q VVR+V+LLHL HPGHL RQR E VEADVE HLPE DL R
Sbjct: 378 GLRHGPRQQVVRNVELLHLLHPGHLWRQRPCEGVEADVEHRHLPEHPDLGR 226
>gb|CA007888.1|CA007888 HU09E19r HU Hordeum vulgare subsp. vulgare cDNA clone HU09E19
5-PRIME, mRNA sequence
Length = 516
Score = 200 bits (431), Expect(2) = 4e-058
Identities = 76/92 (82%), Positives = 83/92 (90%)
Frame = +2 / +1
Query: 101 GPLPQQVAGMRKVEQLDVAHNRLSGAVPEAICALPRLKNLTISYNFFTGEPPSCARVVPS 280
GPLP QVAGM+KVEQ D+AHN LSG VPEA+C LPRLKN T SYNFFTGEPPSCARVVP+
Sbjct: 10 GPLPPQVAGMQKVEQFDIAHNLLSGTVPEAVCDLPRLKNFTFSYNFFTGEPPSCARVVPA 189
Query: 281 DGDRRNCLPNRPTQRTPQQCAAFYSQPPVDCA 376
DGDRRNCLP+RP QR PQQCAAFY++PPVDCA
Sbjct: 190 DGDRRNCLPDRPAQRMPQQCAAFYARPPVDCA 285
Score = 164 bits (352), Expect(3) = 1e-045
Identities = 70/93 (75%), Positives = 75/93 (80%)
Frame = -1 / -2
Query: 375 AQSTGGCE*NAAHCCGVRCVGRLGRQFLLSPSEGTTRAQDGGSPVKKL*EMVRFLSRGRA 196
AQSTGG * AAHCCG+R GR GRQF SPS GTTRAQDGGSPVKKL*E V+FLSRGR+
Sbjct: 284 AQSTGGRA*KAAHCCGMRWAGRSGRQFRRSPSAGTTRAQDGGSPVKKL*EKVKFLSRGRS 105
Query: 195 QMASGTAPDRRLCATSSCSTLRIPATCCGSGPT 97
ASGT PD RLCA S+CST IPATC GSGP+
Sbjct: 104 HTASGTVPDSRLCAMSNCSTFCIPATCGGSGPS 6
Score = 145 bits (311), Expect = 2e-034
Identities = 65/89 (73%), Positives = 71/89 (79%)
Frame = -3 / -1
Query: 370 VDGRLRVERGALLRRALCGAVGQAVPPVTVRRHDARAGRRLAGEEVVGDGQVLEPREGAD 191
VDG +E GALLR AL GAVG+AVP V V RHDARAG RLAGEEVVG+G+VLEP E A
Sbjct: 279 VDGWAGIESGALLRHALGGAVGEAVPAVAVGRHDARAGWRLAGEEVVGEGEVLEPWEVAH 100
Query: 190 GLRDGPGQAVVRDVQLLHLAHPGHLLRQR 104
GLR GP Q VVR+V+LLHL HPGHL RQR
Sbjct: 99 GLRHGPRQQVVRNVELLHLLHPGHLWRQR 13
Score = 44.6 bits (91), Expect(2) = 4e-058
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +2 / +3
Query: 374 AIIPRPVATGIPYAVRIASATCAVPMI 454
A IPRPVAT IP+A+R+A+A +PMI
Sbjct: 330 ASIPRPVATSIPHAIRVAAAASTLPMI 410
Score = 37.3 bits (75), Expect(3) = 1e-045
Identities = 17/27 (62%), Positives = 20/27 (74%)
Frame = -1 / -3
Query: 453 IIGTAQVAEAMRTA*GIPVATGRGMMA 373
IIG+ + A A R A GI VATGRGM+A
Sbjct: 409 IIGSVEAAAATRMAWGILVATGRGMLA 329
Score = 21.7 bits (41), Expect(3) = 1e-045
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = -1 / -2
Query: 558 LTTYNCNGEL 529
+TTY C+GEL
Sbjct: 515 MTTYFCDGEL 486
>gb|CA001848.1|CA001848 HS05M10r HS Hordeum vulgare subsp. vulgare cDNA clone HS05M10
5-PRIME, mRNA sequence
Length = 451
Score = 180 bits (387), Expect = 8e-045
Identities = 70/94 (74%), Positives = 81/94 (86%)
Frame = +2 / +1
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
INNGL SC+PPE+G LRE+TV DVS N L GPL Q+VAG+RK+EQL+VAHN LSG +P+A
Sbjct: 169 INNGLKSCLPPEIGRLRELTVLDVSHNQLAGPLTQEVAGLRKLEQLNVAHNLLSGPIPQA 348
Query: 191 ICALPRLKNLTISYNFFTGEPPSCARVVPSDGDR 292
+CALPRLKN T SYNFFTGEPP+CARVVP D DR
Sbjct: 349 VCALPRLKNFTFSYNFFTGEPPACARVVPRDSDR 450
Score = 128 bits (274), Expect = 3e-029
Identities = 59/93 (63%), Positives = 65/93 (69%)
Frame = -1 / -3
Query: 288 SPSEGTTRAQDGGSPVKKL*EMVRFLSRGRAQMASGTAPDRRLCATSSCSTLRIPATCCG 109
S S GTTRA GGSPVKKL E V+FLSRG A A G P+ RLCAT SCS+ PAT C
Sbjct: 446 SLSRGTTRAHAGGSPVKKLYEKVKFLSRGSAHTAWGIGPESRLCATLSCSSFLSPATSCV 267
Query: 108 SGPTSELKLTSKTVTSRSKPTSGGTQESRPLLM 10
SGP S L LTS TV+SR +P SGG + RPLLM
Sbjct: 266 SGPASWLWLTSSTVSSRRRPISGGRHDLRPLLM 168
Score = 111 bits (237), Expect = 4e-024
Identities = 52/84 (61%), Positives = 60/84 (71%)
Frame = -3 / -2
Query: 289 VTVRRHDARAGRRLAGEEVVGDGQVLEPREGADGLRDGPGQAVVRDVQLLHLAHPGHLLR 110
V V RHDARA RRL GEEVV +G+VLE R+ A GL P + VVRDV+LL L P HLLR
Sbjct: 447 VAVPRHDARARRRLPGEEVV*EGEVLESRQRAHGLGYRPREQVVRDVELLQLPQPRHLLR 268
Query: 109 QRANERVEADVEDGHLPEQADLRR 38
+RA E V AD+E LP+ ADLRR
Sbjct: 267 ERAGELVVADIEHRELPQAADLRR 196
>gb|BM373115.2|BM373115 EBma04_SQ003_C15_R maternal, 10 DPA, no treatment, cv Optic, EBma04
Hordeum vulgare subsp. vulgare cDNA clone
EBma04_SQ003_C15 5', mRNA sequence
Length = 486
Score = 151 bits (325), Expect(2) = 1e-043
Identities = 56/66 (84%), Positives = 61/66 (92%)
Frame = +2 / +1
Query: 179 VPEAICALPRLKNLTISYNFFTGEPPSCARVVPSDGDRRNCLPNRPTQRTPQQCAAFYSQ 358
VPEA+C LPRLKN T SYNFFTGEPPSCARVVP+DGDRRNCLP+RP QR PQQCAAFY++
Sbjct: 1 VPEAVCDLPRLKNFTFSYNFFTGEPPSCARVVPADGDRRNCLPDRPAQRMPQQCAAFYAR 180
Query: 359 PPVDCA 376
PPVDCA
Sbjct: 181 PPVDCA 198
Score = 114 bits (244), Expect(4) = 2e-034
Identities = 50/65 (76%), Positives = 53/65 (81%)
Frame = -1 / -2
Query: 375 AQSTGGCE*NAAHCCGVRCVGRLGRQFLLSPSEGTTRAQDGGSPVKKL*EMVRFLSRGRA 196
AQSTGG * AAHCCG+R GR GRQF SPS GTTRAQDGGSPVKKL*E V+FLSRGR+
Sbjct: 197 AQSTGGRA*KAAHCCGMRWAGRSGRQFRRSPSAGTTRAQDGGSPVKKL*EKVKFLSRGRS 18
Query: 195 QMASG 181
ASG
Sbjct: 17 HTASG 3
Score = 44.6 bits (91), Expect(2) = 1e-043
Identities = 17/27 (62%), Positives = 22/27 (81%)
Frame = +2 / +3
Query: 374 AIIPRPVATGIPYAVRIASATCAVPMI 454
A IPRPVAT IP+A+R+A+A +PMI
Sbjct: 243 ASIPRPVATSIPHAIRVAAAASTLPMI 323
Score = 37.3 bits (75), Expect(4) = 2e-034
Identities = 17/27 (62%), Positives = 20/27 (74%)
Frame = -1 / -3
Query: 453 IIGTAQVAEAMRTA*GIPVATGRGMMA 373
IIG+ + A A R A GI VATGRGM+A
Sbjct: 322 IIGSVEAAAATRMAWGILVATGRGMLA 242
Score = 28.1 bits (55), Expect(4) = 2e-034
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = -1 / -2
Query: 615 LLYAGSYQSRT 583
LLYAGSYQSRT
Sbjct: 479 LLYAGSYQSRT 447
Score = 21.7 bits (41), Expect(4) = 2e-034
Identities = 7/10 (70%), Positives = 9/10 (90%)
Frame = -1 / -2
Query: 558 LTTYNCNGEL 529
+TTY C+GEL
Sbjct: 428 MTTYFCDGEL 399
>gb|CB876810.1|CB876810 HP02H19T HP Hordeum vulgare subsp. vulgare cDNA clone HP02H19
5-PRIME, mRNA sequence
Length = 648
Score = 136 bits (291), Expect(2) = 2e-039
Identities = 54/91 (59%), Positives = 67/91 (73%)
Frame = +2 / +2
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
IN+G+ SC+P E+G LRE+TV DVSFN L G LP+ +AGM +EQLDVAHN L+G +PE
Sbjct: 221 INSGIRSCIPQEIGWLRELTVLDVSFNQLQGTLPESMAGMHALEQLDVAHNELTGHIPEG 400
Query: 191 ICALPRLKNLTISYNFFTGEPPSCARVVPSD 283
ICALPRL N T SYN+F GE C + +D
Sbjct: 401 ICALPRLANFTYSYNYFCGELERCTALRRND 493
Score = 46.4 bits (95), Expect(2) = 2e-039
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +2 / +2
Query: 287 DRRNCLPNRPTQRTPQQCAAFYSQPPVDC 373
DR+NC+ RP QR QC AF +PPV C
Sbjct: 494 DRQNCIAGRPDQRPADQCLAFLHRPPVHC 580
>gb|CB879498.1|CB879498 HP02H19w HP Hordeum vulgare subsp. vulgare cDNA clone HP02H19
3-PRIME, mRNA sequence
Length = 648
Score = 136 bits (291), Expect(2) = 2e-039
Identities = 54/91 (59%), Positives = 67/91 (73%)
Frame = +2 / -2
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
IN+G+ SC+P E+G LRE+TV DVSFN L G LP+ +AGM +EQLDVAHN L+G +PE
Sbjct: 479 INSGIRSCIPQEIGWLRELTVLDVSFNQLQGTLPESMAGMHALEQLDVAHNELTGHIPEG 300
Query: 191 ICALPRLKNLTISYNFFTGEPPSCARVVPSD 283
ICALPRL N T SYN+F GE C + +D
Sbjct: 299 ICALPRLANFTYSYNYFCGELERCTALRRND 207
Score = 46.4 bits (95), Expect(2) = 2e-039
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +2 / -2
Query: 287 DRRNCLPNRPTQRTPQQCAAFYSQPPVDC 373
DR+NC+ RP QR QC AF +PPV C
Sbjct: 206 DRQNCIAGRPDQRPADQCLAFLHRPPVHC 120
>gb|BI947034.1|BI947034 HVSMEl0003F09f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0003F09f, mRNA sequence
Length = 1107
Score = 150 bits (323), Expect = 5e-036
Identities = 63/86 (73%), Positives = 70/86 (81%)
Frame = +2 / +1
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
INNGL SC+P EVG+LREVTVFDVSFN+L GPLP QVAGM+KVE +D+AHN LSG VPEA
Sbjct: 184 INNGLTSCLPAEVGMLREVTVFDVSFNALAGPLPPQVAGMQKVEHVDIAHNLLSGTVPEA 363
Query: 191 ICALPRLKNLTISYNFFTGEPPSCAR 268
+C LPRLK LT S NF TG P AR
Sbjct: 364 VCHLPRLKKLTFSTNFSTGSPILGAR 441
Score = 125 bits (267), Expect = 3e-028
Identities = 58/86 (67%), Positives = 60/86 (69%)
Frame = -1 / -2
Query: 267 RAQDGGSPVKKL*EMVRFLSRGRAQMASGTAPDRRLCATSSCSTLRIPATCCGSGPTSEL 88
RA G PV+K E V FLSRGR ASGT PD RLCA S+CST IPATC GSGP L
Sbjct: 440 RAPRMGLPVEKFVEKVSFLSRGRWHTASGTVPDSRLCAMSTCSTFCIPATCGGSGPARAL 261
Query: 87 KLTSKTVTSRSKPTSGGTQESRPLLM 10
KLTS TVTSRS PTS G E RPLLM
Sbjct: 260 KLTSNTVTSRSIPTSAGRHEVRPLLM 183
>gb|BF624819.3|BF624819 HVSMEa0017N07f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0017N07f, mRNA sequence
Length = 672
Score = 140 bits (301), Expect = 6e-033
Identities = 55/91 (60%), Positives = 68/91 (74%)
Frame = +2 / +3
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
IN+G+ SC+P E+G LRE+TV DVSFN L G LP+ +AGM +EQLDVAHN L+G +PE
Sbjct: 96 INSGIRSCIPQEIGWLRELTVLDVSFNQLQGTLPESMAGMHALEQLDVAHNELTGHIPEG 275
Query: 191 ICALPRLKNLTISYNFFTGEPPSCARVVPSD 283
ICALPRL N T SYN+F GEP C + +D
Sbjct: 276 ICALPRLANFTYSYNYFCGEPERCTALRRND 368
>gb|BF255465.2|BF255465 HVSMEf0006O24f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0006O24f, mRNA sequence
Length = 603
Score = 140 bits (301), Expect = 6e-033
Identities = 55/91 (60%), Positives = 68/91 (74%)
Frame = +2 / +2
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
IN+G+ SC+P E+G LRE+TV DVSFN L G LP+ +AGM +EQLDVAHN L+G +PE
Sbjct: 197 INSGIRSCIPQEIGWLRELTVLDVSFNQLQGTLPESMAGMHALEQLDVAHNELTGHIPEG 376
Query: 191 ICALPRLKNLTISYNFFTGEPPSCARVVPSD 283
ICALPRL N T SYN+F GEP C + +D
Sbjct: 377 ICALPRLANFTYSYNYFCGEPERCTALRRND 469
>gb|BF260744.3|BF260744 HVSMEf0022N07f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0022N07f, mRNA sequence
Length = 674
Score = 140 bits (301), Expect = 6e-033
Identities = 55/91 (60%), Positives = 68/91 (74%)
Frame = +2 / +3
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
IN+G+ SC+P E+G LRE+TV DVSFN L G LP+ +AGM +EQLDVAHN L+G +PE
Sbjct: 96 INSGIRSCIPQEIGWLRELTVLDVSFNQLQGTLPESMAGMHALEQLDVAHNELTGHIPEG 275
Query: 191 ICALPRLKNLTISYNFFTGEPPSCARVVPSD 283
ICALPRL N T SYN+F GEP C + +D
Sbjct: 276 ICALPRLANFTYSYNYFCGEPERCTALRRND 368
>gb|BQ758997.1|BQ758997 EBma07_SQ003_P08_R maternal, 21 DPA, no treatment, cv Optic, EBma07
Hordeum vulgare subsp. vulgare cDNA clone
EBma07_SQ003_P08 5', mRNA sequence
Length = 279
Score = 138 bits (295), Expect = 4e-032
Identities = 51/59 (86%), Positives = 55/59 (93%)
Frame = +2 / +3
Query: 200 LPRLKNLTISYNFFTGEPPSCARVVPSDGDRRNCLPNRPTQRTPQQCAAFYSQPPVDCA 376
LPRLKN T SYNFFTGEPPSCARVVP+DGDRRNCLP+RP QR PQQCAAFY++PPVDCA
Sbjct: 6 LPRLKNFTFSYNFFTGEPPSCARVVPADGDRRNCLPDRPAQRMPQQCAAFYARPPVDCA 182
Score = 108 bits (231), Expect(2) = 1e-025
Identities = 47/60 (78%), Positives = 50/60 (83%)
Frame = -1 / -3
Query: 375 AQSTGGCE*NAAHCCGVRCVGRLGRQFLLSPSEGTTRAQDGGSPVKKL*EMVRFLSRGRA 196
AQSTGG * AAHCCG+R GR GRQF SPS GTTRAQDGGSPVKKL*E V+FLSRGR+
Sbjct: 181 AQSTGGRA*KAAHCCGMRWAGRSGRQFRRSPSAGTTRAQDGGSPVKKL*EKVKFLSRGRS 2
Score = 26.7 bits (52), Expect(2) = 1e-025
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = -1 / -3
Query: 420 RTA*GIPVATGRGMMA 373
R A GI VATGRGM+A
Sbjct: 274 RMAWGILVATGRGMLA 227
>gb|BU969789.1|BU969789 HB12J02r BC Hordeum vulgare subsp. vulgare cDNA clone HB12J02
5-PRIME, mRNA sequence
Length = 421
Score = 113 bits (241), Expect = 1e-024
Identities = 43/91 (47%), Positives = 60/91 (65%)
Frame = +2 / +2
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
+N + SC+PPE+G L+++ V D+S N G LP+ + M +E ++V +N L+GAVPEA
Sbjct: 47 LNTSISSCIPPEIGKLKKLKVLDLSHNEFAGELPESIGDMESLEVINVGYNMLTGAVPEA 226
Query: 191 ICALPRLKNLTISYNFFTGEPPSCARVVPSD 283
IC LP LKNLT+ N+F EP SC V D
Sbjct: 227 ICLLPNLKNLTVVGNYFCEEPVSCLHVPRRD 319
>gb|BG301296.1|BG301296 HVSMEb0020I18f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0020I18f, mRNA sequence
Length = 890
Score = 78.5 bits (165), Expect(3) = 5e-024
Identities = 30/64 (46%), Positives = 41/64 (64%)
Frame = +2 / +3
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
+NN L CVP E+G+L + TV D N L GP+P + + VEQL++ NRL G VP+A
Sbjct: 9 LNNQLSGCVPHELGMLTKTTVIDAGMNQLTGPIPSSFSCLNSVEQLNLGGNRLYGQVPDA 188
Query: 191 ICAL 202
+C L
Sbjct: 189 LCKL 200
Score = 37.3 bits (75), Expect(3) = 5e-024
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2 / +3
Query: 296 NCLPNRPTQRTPQQCAAFYSQP 361
NC+P QR P +CA+F SQP
Sbjct: 312 NCIPGMANQRAPAECASFLSQP 377
Score = 32.2 bits (64), Expect(3) = 5e-024
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2 / +3
Query: 206 RLKNLTISYNFFTGEPPSCARVV 274
RL NLT+S N+FT P+C+ ++
Sbjct: 216 RLSNLTLSGNYFTSVGPACSALI 284
>gb|BG299925.1|BG299925 HVSMEa0022I12f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0022I12f, mRNA sequence
Length = 544
Score = 78.5 bits (165), Expect(3) = 5e-024
Identities = 30/64 (46%), Positives = 41/64 (64%)
Frame = +2 / +1
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
+NN L CVP E+G+L + TV D N L GP+P + + VEQL++ NRL G VP+A
Sbjct: 49 LNNQLSGCVPHELGMLTKTTVIDAGMNQLTGPIPSSFSCLNSVEQLNLGGNRLYGQVPDA 228
Query: 191 ICAL 202
+C L
Sbjct: 229 LCKL 240
Score = 37.3 bits (75), Expect(3) = 5e-024
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2 / +1
Query: 296 NCLPNRPTQRTPQQCAAFYSQP 361
NC+P QR P +CA+F SQP
Sbjct: 352 NCIPGMANQRAPAECASFLSQP 417
Score = 32.2 bits (64), Expect(3) = 5e-024
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2 / +1
Query: 206 RLKNLTISYNFFTGEPPSCARVV 274
RL NLT+S N+FT P+C+ ++
Sbjct: 256 RLSNLTLSGNYFTSVGPACSALI 324
>gb|BF627500.2|BF627500 HVSMEb0005A10f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0005A10f, mRNA sequence
Length = 758
Score = 77.1 bits (162), Expect(3) = 1e-023
Identities = 29/64 (45%), Positives = 42/64 (65%)
Frame = +2 / +1
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
+NN L C+P E+G+L + TV D N + GP+P + + VEQL++A NRL G VP+A
Sbjct: 196 LNNQLSGCLPNELGMLTKTTVIDAGMNQITGPIPSSFSCLGSVEQLNLAGNRLYGQVPDA 375
Query: 191 ICAL 202
+C L
Sbjct: 376 LCKL 387
Score = 36.3 bits (73), Expect(3) = 1e-023
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = +2 / +1
Query: 290 RRNCLPNRPTQRTPQQCAAFYSQP 361
+ NC+P QR P +CA+F SQP
Sbjct: 493 KHNCIPGFANQRGPAECASFLSQP 564
Score = 33.6 bits (67), Expect(3) = 1e-023
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +2 / +1
Query: 206 RLKNLTISYNFFTGEPPSCARVV 274
RL NLT+S N+FT P+CA +V
Sbjct: 403 RLANLTLSGNYFTSVGPACAALV 471
>gb|BF630433.1|BF630433 HVSMEb0009F21f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0009F21f, mRNA sequence
Length = 733
Score = 73.9 bits (155), Expect(3) = 1e-022
Identities = 29/63 (46%), Positives = 38/63 (60%)
Frame = +2 / -3
Query: 14 NNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEAI 193
NN CVP E+G+L + TV D N L GP+P + + VEQL+ NRL G VP+A+
Sbjct: 713 NNSFSGCVPHELGMLTKTTVXDAGMNQLNGPIPSSFSCLNSVEQLNXGGNRLYGQVPDAL 534
Query: 194 CAL 202
C L
Sbjct: 533 CKL 525
Score = 37.3 bits (75), Expect(3) = 1e-022
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2 / -3
Query: 296 NCLPNRPTQRTPQQCAAFYSQP 361
NC+P QR P +CA+F SQP
Sbjct: 413 NCIPGMANQRAPAECASFLSQP 348
Score = 32.2 bits (64), Expect(3) = 1e-022
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2 / -3
Query: 206 RLKNLTISYNFFTGEPPSCARVV 274
RL NLT+S N+FT P+C+ ++
Sbjct: 509 RLSNLTLSGNYFTSVGPACSALI 441
>gb|BJ466753.1|BJ466753 BJ466753 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags33m08 3', mRNA sequence
Length = 697
Score = 73.0 bits (153), Expect(3) = 2e-022
Identities = 28/60 (46%), Positives = 38/60 (63%)
Frame = +2 / -1
Query: 23 LDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEAICAL 202
L CVP E+G+L + TV D N L GP+P + + VEQL++ NRL G VP+A+C L
Sbjct: 682 LSGCVPHELGMLTKTTVIDAGMNQLTGPIPSSFSCLNSVEQLNLGGNRLYGQVPDALCKL 503
Score = 37.3 bits (75), Expect(3) = 2e-022
Identities = 12/22 (54%), Positives = 15/22 (68%)
Frame = +2 / -1
Query: 296 NCLPNRPTQRTPQQCAAFYSQP 361
NC+P QR P +CA+F SQP
Sbjct: 391 NCIPGMANQRAPAECASFLSQP 326
Score = 32.2 bits (64), Expect(3) = 2e-022
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2 / -1
Query: 206 RLKNLTISYNFFTGEPPSCARVV 274
RL NLT+S N+FT P+C+ ++
Sbjct: 487 RLSNLTLSGNYFTSVGPACSALI 419
>gb|BU970614.1|BU970614 HB15C18r BC Hordeum vulgare subsp. vulgare cDNA clone HB15C18
5-PRIME, mRNA sequence
Length = 511
Score = 103 bits (220), Expect = 8e-022
Identities = 40/63 (63%), Positives = 50/63 (79%)
Frame = +2 / +3
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
IN+G+ SC+P E+G LRE+TV DVSFN L G LP+ +AGM +EQLDVAHN L+G +PE
Sbjct: 321 INSGIRSCIPQEIGWLRELTVLDVSFNQLQGTLPESMAGMHALEQLDVAHNELTGHIPEG 500
Query: 191 ICA 199
ICA
Sbjct: 501 ICA 509
>gb|BF621296.2|BF621296 HVSMEa0006G08f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0006G08f, mRNA sequence
Length = 822
Score = 73.5 bits (154), Expect(3) = 1e-021
Identities = 27/64 (42%), Positives = 41/64 (64%)
Frame = +2 / +2
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
+NN L C+P E+G+L + TV D N + GP+P + + V+QL++A NRL G VP+
Sbjct: 275 LNNQLSGCLPNELGMLTKTTVIDAGMNQITGPIPSSFSCLGSVKQLNLAGNRLYGQVPDT 454
Query: 191 ICAL 202
+C L
Sbjct: 455 LCKL 466
Score = 33.6 bits (67), Expect(3) = 1e-021
Identities = 13/23 (56%), Positives = 17/23 (73%)
Frame = +2 / +2
Query: 206 RLKNLTISYNFFTGEPPSCARVV 274
RL NLT+S N+FT P+CA +V
Sbjct: 482 RLANLTLSGNYFTSVGPACAALV 550
Score = 32.7 bits (65), Expect(3) = 1e-021
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +2 / +2
Query: 290 RRNCLPNRPTQRTPQQCAAFYSQP 361
+ NC+P QR P +CA + SQP
Sbjct: 572 KHNCIPGFANQRGPTECA*YLSQP 643
>gb|BU970588.1|BU970588 HB15B14r BC Hordeum vulgare subsp. vulgare cDNA clone HB15B14
5-PRIME, mRNA sequence
Length = 508
Score = 101 bits (216), Expect = 3e-021
Identities = 39/62 (62%), Positives = 49/62 (79%)
Frame = +2 / +3
Query: 11 INNGLDSCVPPEVGLLREVTVFDVSFNSLVGPLPQQVAGMRKVEQLDVAHNRLSGAVPEA 190
IN+G+ SC+P E+G LRE+TV DVSFN L G LP+ +AGM +EQLDVAHN L+G +PE
Sbjct: 321 INSGIRSCIPQEIGWLRELTVLDVSFNQLQGTLPESMAGMHALEQLDVAHNELTGHIPEG 500
Query: 191 IC 196
IC
Sbjct: 501 IC 506
>gb|BU968318.1|BU968318 HB07C12r BC Hordeum vulgare subsp. vulgare cDNA clone HB07C12
5-PRIME, mRNA sequence
Length = 504
Score = 73.9 bits (155), Expect(2) = 5e-021
Identities = 29/49 (59%), Positives = 35/49 (71%)
Frame = +2 / +1
Query: 137 VEQLDVAHNRLSGAVPEAICALPRLKNLTISYNFFTGEPPSCARVVPSD 283
+EQLDVAHN L+G +PE ICALPRL N T SYN+F GE C + +D
Sbjct: 16 LEQLDVAHNELTGHIPEGICALPRLANFTYSYNYFCGELERCTALRRND 162
Score = 46.4 bits (95), Expect(2) = 5e-021
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +2 / +1
Query: 287 DRRNCLPNRPTQRTPQQCAAFYSQPPVDC 373
DR+NC+ RP QR QC AF +PPV C
Sbjct: 163 DRQNCIAGRPDQRPADQCLAFLHRPPVHC 249
>gb|BQ472572.1|BQ472572 HB09C12T BC Hordeum vulgare subsp. vulgare cDNA clone HB09C12
5-PRIME, mRNA sequence
Length = 486
Score = 73.9 bits (155), Expect(2) = 5e-021
Identities = 29/49 (59%), Positives = 35/49 (71%)
Frame = +2 / +1
Query: 137 VEQLDVAHNRLSGAVPEAICALPRLKNLTISYNFFTGEPPSCARVVPSD 283
+EQLDVAHN L+G +PE ICALPRL N T SYN+F GE C + +D
Sbjct: 16 LEQLDVAHNELTGHIPEGICALPRLANFTYSYNYFCGELERCTALRRND 162
Score = 46.4 bits (95), Expect(2) = 5e-021
Identities = 16/29 (55%), Positives = 19/29 (65%)
Frame = +2 / +1
Query: 287 DRRNCLPNRPTQRTPQQCAAFYSQPPVDC 373
DR+NC+ RP QR QC AF +PPV C
Sbjct: 163 DRQNCIAGRPDQRPADQCLAFLHRPPVHC 249
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 215,162,616
Number of Sequences: 312970
Number of extensions: 3297937
Number of successful extensions: 61999
Number of sequences better than 1.0e-020: 27
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6913923567
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)