TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCS16e03.yg.2.1
(608 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA007616.1|CA007616 HU08I04r HU Hordeum vulgare subsp. v... 235 1e-061
gb|AV836714.1|AV836714 AV836714 K. Sato unpublished cDNA li... 124 2e-035
gb|CA018111.1|CA018111 HV07H21r HV Hordeum vulgare subsp. v... 133 8e-031
>gb|CA007616.1|CA007616 HU08I04r HU Hordeum vulgare subsp. vulgare cDNA clone HU08I04
5-PRIME, mRNA sequence
Length = 622
Score = 235 bits (508), Expect = 1e-061
Identities = 99/130 (76%), Positives = 114/130 (87%)
Frame = -2 / +1
Query: 406 FSRELNSSEMPFAPIASDSISLIISVEDTGVGIPFDAQSRVFTPFMQVGPSIARIHGGTG 227
F+ +L+SSE+PFAPI ++I LIISVEDTGVGIP +AQSR+FTPFMQVGPSIARIHGGTG
Sbjct: 16 FNMDLHSSELPFAPIMPETIRLIISVEDTGVGIPLEAQSRIFTPFMQVGPSIARIHGGTG 195
Query: 226 IGLSISKCLVGLMRGEIGFASKPQVGSTFTFTAVLTRAHSSRNENKSSEFKGINALVVDH 47
IGLSISKCLV LM+GEIGF SK VGSTF+FTAVL+RA + N NKS FKG++ALVVDH
Sbjct: 196 IGLSISKCLVHLMKGEIGFVSKHHVGSTFSFTAVLSRARPNANVNKSPGFKGVSALVVDH 375
Query: 46 RPVRAKVTRY 17
RPVRA VT+Y
Sbjct: 376 RPVRANVTKY 405
Score = 222 bits (480), Expect = 1e-057
Identities = 99/130 (76%), Positives = 104/130 (80%)
Frame = +3 / -3
Query: 18 YLVTLARTGL*STTNALIPLNSDDLFSFLLEWALVRTAVKVKVEPTWGLLANPISPLMSP 197
YLVTLARTGL*STTNAL PLN DL +F L AL TAVK VEPTW LL NPISP M
Sbjct: 404 YLVTLARTGL*STTNALTPLNPGDLLTFALGRALESTAVKENVEPTWCLLTNPISPFMR* 225
Query: 198 TKHLLMLNPMPVPPWMRAMDGPTCMKGVKTRD*ASNGIPTPVSSTDIIKLIESDAMGAKG 377
T+HLLMLNPMPVPPWMRAMDGPTCMKGV RD AS+G+PTPVSSTDII LI S MGAKG
Sbjct: 224 TRHLLMLNPMPVPPWMRAMDGPTCMKGVNMRDWASSGMPTPVSSTDIINLIVSGMMGAKG 45
Query: 378 ISDEFSSLLK 407
SDE S+LK
Sbjct: 44 NSDECRSILK 15
Score = 177 bits (381), Expect = 5e-044
Identities = 69/125 (55%), Positives = 86/125 (68%)
Frame = -1 / +3
Query: 398 GTELI*DAFCTHRIRLNKLDNIS*RYGCWNPVRCSVPCLHPFHASRSIHCSHPWGHWHWI 219
G+ L+ A C+H +K+DNIS*RY C +P +P +H FHA S+HC HPWGHWH I
Sbjct: 24 GSTLVRVALCSHHA*NDKIDNIS*RYWCRHPA*SPIPHIHAFHAGWSVHCPHPWGHWHRI 203
Query: 218 KHQQMLGWTHEGRDWICK*TPSWFYFHLHCGSYEGPFQQK*E*IVRI*RDQCIGGRS*AG 39
+HQQ+ G +HEG DW+CK TP WFY LHC ++EG Q K + I R+*R QCIG S*AG
Sbjct: 204 EHQQVPGSSHEGGDWVCKQTPCWFYVLLHCSTFEGTPQCKCQQITRV*RGQCIGS*S*AG 383
Query: 38 PCQGY 24
CQ Y
Sbjct: 384 SCQCY 398
Score = 151 bits (325), Expect = 3e-036
Identities = 72/127 (56%), Positives = 84/127 (66%)
Frame = +2 / -2
Query: 2 RPPGQVPSNLGTDRPMIYHQCIDPFKF*RFILISAGMGPRKNRSEGESRTNLGFTCKSNL 181
+P + N+GT+RPMI +QC DPFK F+ I G PRK SEGE RTN+ F K NL
Sbjct: 420 KPL*MILGNIGTNRPMINYQCTDPFKPW*FVDICIGACPRKYCSEGERRTNMVFAYKPNL 241
Query: 182 SPHESNQAFADA*SNASAPMDASNGWTYLHERGEDTGLSIERDSNTRIFN*YYQAY*V*C 361
HE NQA ADA S+ASAPMDA NG T LHER E GL +RD++T IFN*YYQ+Y
Sbjct: 240 PLHEMNQALADAQSDASAPMDAGNGRTNLHERREYAGLGFKRDADTSIFN*YYQSYRFRH 61
Query: 362 DGCKRHL 382
DG K L
Sbjct: 60 DGSKGQL 40
Score = 129 bits (276), Expect = 1e-029
Identities = 70/122 (57%), Positives = 78/122 (63%)
Frame = -3 / +2
Query: 390 THLRCLLHPSHQTQ*A**YQLKIRVLESRSMLSPVSSPLSCK*VHPLLASMGALALD*AS 211
T C L PS +* **YQLKI V SR +P S LSC+ V PL ASMGALA D*AS
Sbjct: 32 TRPSCPLLPSCLKR*D**YQLKILVSASRLKPNPAYSRLSCRLVRPLPASMGALASD*AS 211
Query: 210 ANAWLDS*GERLDLQVNPKLVLLSPSLRFLRGPIPAEMRINRQNLKGSMHWW*IIGRSVP 31
A+AW S* RL L N LVL SPSL++ RG P +M N Q LKGS+HW IIGR VP
Sbjct: 212 ASAWFIS*RGRLGL*ANTMLVLRSPSLQYFRGHAPMQMSTNHQGLKGSVHW*LIIGRFVP 391
Query: 30 RL 25
L
Sbjct: 392 ML 397
Score = 121 bits (259), Expect = 3e-027
Identities = 62/126 (49%), Positives = 70/126 (55%)
Frame = +1 / -1
Query: 31 WHGPAYDLPPMH*SL*ILTIYSHFCWNGPS*EPQ*R*K*NQLGVYLQIQSLPS*VQPSIC 210
WH PAYD PMH* L* L I H W PS Q*R *NQ GV LQ QS PS* +P C
Sbjct: 391 WHEPAYDQLPMH*PL*TLVIC*HLHWGVPSKVLQ*RRT*NQHGVCLQTQSPPS*DEPGTC 212
Query: 211 *CLIQCQCPHGCEQWMDLLA*KG*RHGTEHRTGFQHPYXXXXXXXXXXXMRWVQKASQMS 390
*C I+CQCPHGC QW D A*K * G + G +H Y W Q+A++ S
Sbjct: 211 *CSIRCQCPHGCGQWTDQPA*KA*ICGIGLQAGCRHQYLQLILSILSFQA*WEQRATRTS 32
Query: 391 SVPC*R 408
P *+
Sbjct: 31 VDPY*K 14
>gb|AV836714.1|AV836714 AV836714 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd26p01, mRNA
sequence
Length = 635
Score = 124 bits (266), Expect(2) = 2e-035
Identities = 50/71 (70%), Positives = 62/71 (87%)
Frame = -2 / +3
Query: 466 NTLSGYPVANRKRSWENFRVFSRELNSSEMPFAPIASDSISLIISVEDTGVGIPFDAQSR 287
NTLSG VA R+ SW++FR+F+ +L+SSE+PFAPI ++I LIISVEDTGVGIP +AQSR
Sbjct: 423 NTLSGCEVAKRRLSWQSFRLFNMDLHSSELPFAPIMPETIRLIISVEDTGVGIPLEAQSR 602
Query: 286 VFTPFMQVGPS 254
+FTPFMQVGPS
Sbjct: 603 IFTPFMQVGPS 635
Score = 110 bits (234), Expect(2) = 2e-026
Identities = 50/71 (70%), Positives = 54/71 (76%)
Frame = +3 / -2
Query: 255 DGPTCMKGVKTRD*ASNGIPTPVSSTDIIKLIESDAMGAKGISDEFSSLLKTRKFSQLRF 434
DGPTCMKGV RD AS+G+PTPVSSTDII LI S MGAKG SDE S+LK+RK QL
Sbjct: 634 DGPTCMKGVNMRDWASSGMPTPVSSTDIINLIVSGMMGAKGNSDECRSILKSRKLCQLSL 455
Query: 435 LFATG*PLKVL 467
L AT PLKVL
Sbjct: 454 LLATSQPLKVL 422
Score = 43.7 bits (89), Expect(2) = 2e-035
Identities = 20/41 (48%), Positives = 23/41 (56%)
Frame = -2 / +3
Query: 592 QIXXXXXXXSXXXTEXXHIYXTVHVVEEIMXXLEXETGTQS 470
QI S TE HIY TVHVVEE+M L+ ET Q+
Sbjct: 294 QIITNLMGNSIKFTERGHIYLTVHVVEEVMCSLDVETEIQN 416
Score = 28.6 bits (56), Expect(2) = 2e-026
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = +2 / -1
Query: 503 HNLFNNMNCXVDMXXLC 553
H+LF+NMN +DM C
Sbjct: 383 HHLFDNMNRQIDMSSFC 333
>gb|CA018111.1|CA018111 HV07H21r HV Hordeum vulgare subsp. vulgare cDNA clone HV07H21
5-PRIME, mRNA sequence
Length = 241
Score = 133 bits (285), Expect = 8e-031
Identities = 56/74 (75%), Positives = 62/74 (83%)
Frame = -2 / +2
Query: 307 PFDAQSRVFTPFMQVGPSIARIHGGTGIGLSISKCLVGLMRGEIGFASKPQVGSTFTFTA 128
P +AQSR+FTPFMQVGPSIARIHGGT IGLSISKCLV LM+GEIGF SK VG F+FTA
Sbjct: 11 PLEAQSRIFTPFMQVGPSIARIHGGTXIGLSISKCLVHLMKGEIGFVSKHHVGXXFSFTA 190
Query: 127 VLTRAHSSRNENKS 86
VL+RA + N NKS
Sbjct: 191 VLSRARPNANVNKS 232
Score = 122 bits (262), Expect = 1e-027
Identities = 52/75 (69%), Positives = 56/75 (74%)
Frame = +3 / -2
Query: 87 DLFSFLLEWALVRTAVKVKVEPTWGLLANPISPLMSPTKHLLMLNPMPVPPWMRAMDGPT 266
DL +F L AL TAVK PTW LL NPISP M T+HLLMLNPM VPPWMRAMDGPT
Sbjct: 231 DLLTFALGRALESTAVKENXXPTWCLLTNPISPFMR*TRHLLMLNPMXVPPWMRAMDGPT 52
Query: 267 CMKGVKTRD*ASNGI 311
CMKGV RD AS+G+
Sbjct: 51 CMKGVNMRDWASSGL 7
Score = 106 bits (226), Expect = 1e-022
Identities = 38/71 (53%), Positives = 48/71 (67%)
Frame = -1 / +1
Query: 293 VPCLHPFHASRSIHCSHPWGHWHWIKHQQMLGWTHEGRDWICK*TPSWFYFHLHCGSYEG 114
+P +H FHA S+HC HPWGH H I+HQQ+ G +HEG DW+CK TP W LHC ++EG
Sbjct: 25 IPHIHAFHAGWSVHCPHPWGHXHRIEHQQVPGSSHEGGDWVCKQTPCWXXVLLHCSTFEG 204
Query: 113 PFQQK*E*IVR 81
Q K + I R
Sbjct: 205 TPQCKCQQITR 237
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 257,978,680
Number of Sequences: 312970
Number of extensions: 4084748
Number of successful extensions: 48172
Number of sequences better than 1.0e-020: 5
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6404923059
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)