TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN28g01.yg.2.1
(513 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 145 7e-045
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 145 bits (312), Expect(2) = 7e-045
Identities = 61/95 (64%), Positives = 72/95 (75%)
Frame = -1 / +3
Query: 495 LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 316
L LLT+KESA +VP NL+A RR+ FF NSLFM MP A V +ML FSV TPY E VL+
Sbjct: 3168 LHSLLTIKESAANVPKNLEASRRLQFFTNSLFMRMPVARPVSEMLSFSVFTPYCSETVLY 3347
Query: 315 SSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVH 211
S L ++NEDG+S LFYLQKIYPDEWKNFL R++
Sbjct: 3348 SIAELQKKNEDGISTLFYLQKIYPDEWKNFLTRIN 3452
Score = 54.2 bits (112), Expect(2) = 7e-045
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = -1 / +3
Query: 204 SEDQLHETEHSEEQLRLWASYRGQTLTRTVRGMMYQER 91
++ +L + + +LRLWASYRGQTL RTVRGMMY +
Sbjct: 3468 ADSELFSSANDILELRLWASYRGQTLARTVRGMMYYRK 3581
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 221,066,305
Number of Sequences: 312970
Number of extensions: 3056044
Number of successful extensions: 51845
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5127561480
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)