TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCN28g01.yg.2.1
         (513 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AY177665.1|  Hordeum vulgare subsp. vulgare putative call...   145   7e-045

>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
            complete cds
          Length = 6180

 Score =  145 bits (312), Expect(2) = 7e-045
 Identities = 61/95 (64%), Positives = 72/95 (75%)
 Frame = -1 / +3

                                                                        
Query: 495  LRLLLTVKESAMDVPTNLDARRRISFFANSLFMSMPDAPKVRQMLPFSVLTPYYKEDVLF 316
            L  LLT+KESA +VP NL+A RR+ FF NSLFM MP A  V +ML FSV TPY  E VL+
Sbjct: 3168 LHSLLTIKESAANVPKNLEASRRLQFFTNSLFMRMPVARPVSEMLSFSVFTPYCSETVLY 3347

                                               
Query: 315  SSQALAEQNEDGVSILFYLQKIYPDEWKNFLERVH 211
            S   L ++NEDG+S LFYLQKIYPDEWKNFL R++
Sbjct: 3348 SIAELQKKNEDGISTLFYLQKIYPDEWKNFLTRIN 3452




 Score = 54.2 bits (112), Expect(2) = 7e-045
 Identities = 21/38 (55%), Positives = 28/38 (73%)
 Frame = -1 / +3

                                                  
Query: 204  SEDQLHETEHSEEQLRLWASYRGQTLTRTVRGMMYQER 91
            ++ +L  + +   +LRLWASYRGQTL RTVRGMMY  +
Sbjct: 3468 ADSELFSSANDILELRLWASYRGQTLARTVRGMMYYRK 3581



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 221,066,305
Number of Sequences: 312970
Number of extensions: 3056044
Number of successful extensions: 51845
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5127561480
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)