TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCN21e10.yg.2.1
(247 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA li... 187 1e-047
gb|AV934137.1|AV934137 AV934137 K. Sato unpublished cDNA li... 187 1e-047
gb|BJ470174.1|BJ470174 BJ470174 K. Sato unpublished cDNA li... 187 1e-047
gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA li... 168 6e-042
gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. v... 160 1e-039
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 160 1e-039
gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA li... 158 5e-039
gb|AV932586.1|AV932586 AV932586 K. Sato unpublished cDNA li... 85 1e-036
gb|BI953459.1|BI953459 HVSMEm0012M15f Hordeum vulgare green... 31 1.1
gb|BG416804.1|BG416804 HVSMEk0014J21f Hordeum vulgare testa... 31 1.1
gb|BG369013.2|BG369013 HVSMEi0022E03f Hordeum vulgare 20 DA... 31 1.1
gb|AL499868.1|AL499868 AL499868 Hordeum vulgare Barke etiol... 30 2.1
gb|BI960665.1|BI960665 HVSMEn0025K21f Hordeum vulgare rachi... 30 2.1
gb|BJ456962.1|BJ456962 BJ456962 K. Sato unpublished cDNA li... 30 2.1
gb|BM372458.2|BM372458 EBpi05_SQ003_A09_R pistil, 8 DPA, no... 30 2.1
gb|CD663955.1|CD663955 UCRHV18_07ah11_b1 Drought-stressed D... 25 2.9
gb|BF626883.2|BF626883 HVSMEb0001J13f Hordeum vulgare seedl... 30 2.9
gb|BQ755032.1|BQ755032 EBed02_SQ003_I09_R endosperm, 8 DPA,... 25 4.3
gb|BQ754874.1|BQ754874 EBed02_SQ003_B03_R endosperm, 8 DPA,... 25 5.0
gb|BM372510.2|BM372510 EBpi05_SQ003_C18_R pistil, 8 DPA, no... 25 5.3
gb|AL511638.1|AL511638 AL511638 Hordeum vulgare Barke devel... 25 5.4
gb|BQ663701.1|BQ663701 HU03K06u HU Hordeum vulgare subsp. v... 29 5.5
gb|BQ465877.1|BQ465877 HU04P01r HU Hordeum vulgare subsp. v... 25 5.5
gb|BU996870.1|BU996870 HI06B15r HI Hordeum vulgare subsp. v... 25 5.8
gb|BQ768637.1|BQ768637 EBro08_SQ012_C09_R root, 3 week, dro... 25 6.8
gb|AL504911.1|AL504911 AL504911 Hordeum vulgare Barke roots... 25 6.9
gb|BJ473108.1|BJ473108 BJ473108 K. Sato unpublished cDNA li... 29 7.5
gb|BG369201.1|BG369201 HVSMEi0023C06f Hordeum vulgare 20 DA... 25 7.7
gb|BG367897.2|BG367897 HVSMEi0014H08f Hordeum vulgare 20 DA... 24 8.3
gb|AL507214.1|AL507214 AL507214 Hordeum vulgare Barke devel... 25 9.9
>gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3g22 5', mRNA sequence
Length = 611
Score = 187 bits (403), Expect = 1e-047
Identities = 76/77 (98%), Positives = 76/77 (98%)
Frame = -2 / +2
Query: 246 AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIANGNGEQTLSRDIYRLGHRFDF 67
AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIA GNGEQTLSRDIYRLGHRFDF
Sbjct: 254 AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIAYGNGEQTLSRDIYRLGHRFDF 433
Query: 66 FRMLSCYYTTIGFYFST 16
FRMLSCYYTTIGFYFST
Sbjct: 434 FRMLSCYYTTIGFYFST 484
Score = 113 bits (242), Expect = 2e-025
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = +2 / -3
Query: 17 VLK*KPIVV**QDSILXXXXXXXXXXXXXLSVCSPLPFAIFASNRDI*LRPTSLPLPTCM 196
VLK*KP+VV**QD+IL L VCSPLP+AIFASN +I*LRPTSLPLPTCM
Sbjct: 483 VLK*KPMVV**QDNILKKSKRWPSR*MSRLRVCSPLPYAIFASNSEI*LRPTSLPLPTCM 304
Query: 197 YSW*VTLPSRNVELNPA 247
YS VT PSR+VELNPA
Sbjct: 303 YS*CVTFPSRSVELNPA 253
Score = 101 bits (216), Expect = 6e-022
Identities = 45/77 (58%), Positives = 58/77 (75%)
Frame = -1 / +1
Query: 247 CRIQFHIARRQCYSP*IHASWQRKGCRSQSNISV*GKDSKWQWRTNAEP*HLPARSSL*F 68
C IQF+ A R+ Y+ *IHASWQ K C SQS+ ++*G+DS W RT++EP*HLP S *F
Sbjct: 253 CWIQFNTA*RKRYTS*IHASWQGKRCWSQSDFTI*GEDSIW*RRTDSEP*HLPTWPSF*F 432
Query: 67 LQDAVLLLHNNWFLLQY 17
LQ+ VLLL+ +W LLQ+
Sbjct: 433 LQNVVLLLYYHWLLLQH 483
Score = 99.6 bits (211), Expect = 3e-021
Identities = 46/74 (62%), Positives = 52/74 (70%)
Frame = +1 / -2
Query: 22 EVETNCCVVTRQHPEEIKAMT*PVDVTAQRLFAIAICYLCLKQRYLIETYIPSFANLHVF 201
EV+ N +V QH EEIK M VDVTAQ LFA+ ICYL LK LIET I S ANLHVF
Sbjct: 478 EVKANGSIVAGQHSEEIKTMAKSVDVTAQSLFAVTICYLRLK**NLIETNISSLANLHVF 299
Query: 202 MVSNIAFAQCGIES 243
M+ N++F QC IES
Sbjct: 298 MMCNVSFTQC*IES 257
Score = 86.3 bits (182), Expect = 3e-017
Identities = 43/74 (58%), Positives = 48/74 (64%)
Frame = -3 / +3
Query: 242 DSIPHCAKAMLLTMNTCKLAKEGM*VSIKYLCLRQR*QMAMANKR*AVTSTG*VIALISS 63
DSI HC K L MNTCKLA+E M VSI++ LR+R* M AN+ *AVTST I LISS
Sbjct: 258 DSIQHCVKETLHIMNTCKLAREEMLVSIRFHYLRRR*HMVTANRL*AVTSTDLAIVLISS 437
Query: 62 GCCLVTTQQLVSTS 21
CC T L TS
Sbjct: 438 ECCPATILPLAFTS 479
Score = 82.6 bits (174), Expect = 4e-016
Identities = 41/74 (55%), Positives = 48/74 (64%)
Frame = +3 / -1
Query: 21 *SRNQLLCSNKTAS*RNQSDDLAGRCHGSAFVRHCHLLSLPQTEIFD*DLHPFLCQLACI 200
*S++Q S++T *RNQ+D GRCHGS VR H+LS PQ D*D H F CQLACI
Sbjct: 479 *SKSQW*YSSRTTF*RNQNDGQVGRCHGSESVRRYHMLSSPQIVKSD*DQHLFPCQLACI 300
Query: 201 HGE*HCLRAMWN*I 242
H * L A+ N*I
Sbjct: 299 HDV*RFLHAVLN*I 258
>gb|AV934137.1|AV934137 AV934137 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal11c09 5', mRNA sequence
Length = 589
Score = 187 bits (403), Expect = 1e-047
Identities = 76/77 (98%), Positives = 76/77 (98%)
Frame = -2 / +1
Query: 246 AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIANGNGEQTLSRDIYRLGHRFDF 67
AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIA GNGEQTLSRDIYRLGHRFDF
Sbjct: 25 AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIAYGNGEQTLSRDIYRLGHRFDF 204
Query: 66 FRMLSCYYTTIGFYFST 16
FRMLSCYYTTIGFYFST
Sbjct: 205 FRMLSCYYTTIGFYFST 255
Score = 113 bits (242), Expect = 2e-025
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = +2 / -3
Query: 17 VLK*KPIVV**QDSILXXXXXXXXXXXXXLSVCSPLPFAIFASNRDI*LRPTSLPLPTCM 196
VLK*KP+VV**QD+IL L VCSPLP+AIFASN +I*LRPTSLPLPTCM
Sbjct: 254 VLK*KPMVV**QDNILKKSKRWPSR*MSRLRVCSPLPYAIFASNSEI*LRPTSLPLPTCM 75
Query: 197 YSW*VTLPSRNVELNPA 247
YS VT PSR+VELNPA
Sbjct: 74 YS*CVTFPSRSVELNPA 24
Score = 101 bits (216), Expect = 6e-022
Identities = 45/77 (58%), Positives = 58/77 (75%)
Frame = -1 / +3
Query: 247 CRIQFHIARRQCYSP*IHASWQRKGCRSQSNISV*GKDSKWQWRTNAEP*HLPARSSL*F 68
C IQF+ A R+ Y+ *IHASWQ K C SQS+ ++*G+DS W RT++EP*HLP S *F
Sbjct: 24 CWIQFNTA*RKRYTS*IHASWQGKRCWSQSDFTI*GEDSIW*RRTDSEP*HLPTWPSF*F 203
Query: 67 LQDAVLLLHNNWFLLQY 17
LQ+ VLLL+ +W LLQ+
Sbjct: 204 LQNVVLLLYYHWLLLQH 254
Score = 99.6 bits (211), Expect = 3e-021
Identities = 46/74 (62%), Positives = 52/74 (70%)
Frame = +1 / -2
Query: 22 EVETNCCVVTRQHPEEIKAMT*PVDVTAQRLFAIAICYLCLKQRYLIETYIPSFANLHVF 201
EV+ N +V QH EEIK M VDVTAQ LFA+ ICYL LK LIET I S ANLHVF
Sbjct: 249 EVKANGSIVAGQHSEEIKTMAKSVDVTAQSLFAVTICYLRLK**NLIETNISSLANLHVF 70
Query: 202 MVSNIAFAQCGIES 243
M+ N++F QC IES
Sbjct: 69 MMCNVSFTQC*IES 28
Score = 86.3 bits (182), Expect = 3e-017
Identities = 43/74 (58%), Positives = 48/74 (64%)
Frame = -3 / +2
Query: 242 DSIPHCAKAMLLTMNTCKLAKEGM*VSIKYLCLRQR*QMAMANKR*AVTSTG*VIALISS 63
DSI HC K L MNTCKLA+E M VSI++ LR+R* M AN+ *AVTST I LISS
Sbjct: 29 DSIQHCVKETLHIMNTCKLAREEMLVSIRFHYLRRR*HMVTANRL*AVTSTDLAIVLISS 208
Query: 62 GCCLVTTQQLVSTS 21
CC T L TS
Sbjct: 209 ECCPATILPLAFTS 250
Score = 82.6 bits (174), Expect = 4e-016
Identities = 41/74 (55%), Positives = 48/74 (64%)
Frame = +3 / -1
Query: 21 *SRNQLLCSNKTAS*RNQSDDLAGRCHGSAFVRHCHLLSLPQTEIFD*DLHPFLCQLACI 200
*S++Q S++T *RNQ+D GRCHGS VR H+LS PQ D*D H F CQLACI
Sbjct: 250 *SKSQW*YSSRTTF*RNQNDGQVGRCHGSESVRRYHMLSSPQIVKSD*DQHLFPCQLACI 71
Query: 201 HGE*HCLRAMWN*I 242
H * L A+ N*I
Sbjct: 70 HDV*RFLHAVLN*I 29
>gb|BJ470174.1|BJ470174 BJ470174 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal15l17 5', mRNA sequence
Length = 537
Score = 187 bits (403), Expect = 1e-047
Identities = 76/77 (98%), Positives = 76/77 (98%)
Frame = -2 / +3
Query: 246 AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIANGNGEQTLSRDIYRLGHRFDF 67
AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIA GNGEQTLSRDIYRLGHRFDF
Sbjct: 36 AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIAYGNGEQTLSRDIYRLGHRFDF 215
Query: 66 FRMLSCYYTTIGFYFST 16
FRMLSCYYTTIGFYFST
Sbjct: 216 FRMLSCYYTTIGFYFST 266
Score = 113 bits (242), Expect = 2e-025
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = +2 / -3
Query: 17 VLK*KPIVV**QDSILXXXXXXXXXXXXXLSVCSPLPFAIFASNRDI*LRPTSLPLPTCM 196
VLK*KP+VV**QD+IL L VCSPLP+AIFASN +I*LRPTSLPLPTCM
Sbjct: 265 VLK*KPMVV**QDNILKKSKRWPSR*MSRLRVCSPLPYAIFASNSEI*LRPTSLPLPTCM 86
Query: 197 YSW*VTLPSRNVELNPA 247
YS VT PSR+VELNPA
Sbjct: 85 YS*CVTFPSRSVELNPA 35
Score = 101 bits (216), Expect = 6e-022
Identities = 45/77 (58%), Positives = 58/77 (75%)
Frame = -1 / +2
Query: 247 CRIQFHIARRQCYSP*IHASWQRKGCRSQSNISV*GKDSKWQWRTNAEP*HLPARSSL*F 68
C IQF+ A R+ Y+ *IHASWQ K C SQS+ ++*G+DS W RT++EP*HLP S *F
Sbjct: 35 CWIQFNTA*RKRYTS*IHASWQGKRCWSQSDFTI*GEDSIW*RRTDSEP*HLPTWPSF*F 214
Query: 67 LQDAVLLLHNNWFLLQY 17
LQ+ VLLL+ +W LLQ+
Sbjct: 215 LQNVVLLLYYHWLLLQH 265
Score = 99.6 bits (211), Expect = 3e-021
Identities = 46/74 (62%), Positives = 52/74 (70%)
Frame = +1 / -2
Query: 22 EVETNCCVVTRQHPEEIKAMT*PVDVTAQRLFAIAICYLCLKQRYLIETYIPSFANLHVF 201
EV+ N +V QH EEIK M VDVTAQ LFA+ ICYL LK LIET I S ANLHVF
Sbjct: 260 EVKANGSIVAGQHSEEIKTMAKSVDVTAQSLFAVTICYLRLK**NLIETNISSLANLHVF 81
Query: 202 MVSNIAFAQCGIES 243
M+ N++F QC IES
Sbjct: 80 MMCNVSFTQC*IES 39
Score = 86.3 bits (182), Expect = 3e-017
Identities = 43/74 (58%), Positives = 48/74 (64%)
Frame = -3 / +1
Query: 242 DSIPHCAKAMLLTMNTCKLAKEGM*VSIKYLCLRQR*QMAMANKR*AVTSTG*VIALISS 63
DSI HC K L MNTCKLA+E M VSI++ LR+R* M AN+ *AVTST I LISS
Sbjct: 40 DSIQHCVKETLHIMNTCKLAREEMLVSIRFHYLRRR*HMVTANRL*AVTSTDLAIVLISS 219
Query: 62 GCCLVTTQQLVSTS 21
CC T L TS
Sbjct: 220 ECCPATILPLAFTS 261
Score = 82.6 bits (174), Expect = 4e-016
Identities = 41/74 (55%), Positives = 48/74 (64%)
Frame = +3 / -1
Query: 21 *SRNQLLCSNKTAS*RNQSDDLAGRCHGSAFVRHCHLLSLPQTEIFD*DLHPFLCQLACI 200
*S++Q S++T *RNQ+D GRCHGS VR H+LS PQ D*D H F CQLACI
Sbjct: 261 *SKSQW*YSSRTTF*RNQNDGQVGRCHGSESVRRYHMLSSPQIVKSD*DQHLFPCQLACI 82
Query: 201 HGE*HCLRAMWN*I 242
H * L A+ N*I
Sbjct: 81 HDV*RFLHAVLN*I 40
>gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags28g06 5', mRNA sequence
Length = 666
Score = 168 bits (361), Expect = 6e-042
Identities = 65/77 (84%), Positives = 73/77 (94%)
Frame = -2 / +3
Query: 246 AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIANGNGEQTLSRDIYRLGHRFDF 67
AGFN TLR GNVTHHEY+QVGKGRDVGLNQ+S+FEAK+A+GNGEQTLSRD+YRLGHR DF
Sbjct: 183 AGFNCTLRGGNVTHHEYIQVGKGRDVGLNQVSMFEAKVASGNGEQTLSRDVYRLGHRLDF 362
Query: 66 FRMLSCYYTTIGFYFST 16
FRMLS +YTTIGFYF+T
Sbjct: 363 FRMLSFFYTTIGFYFNT 413
Score = 86.3 bits (182), Expect = 3e-017
Identities = 36/80 (45%), Positives = 48/80 (60%)
Frame = -1 / +2
Query: 247 CRIQFHIARRQCYSP*IHASWQRKGCRSQSNISV*GKDSKWQWRTNAEP*HLPARSSL*F 68
C IQ + QCY+P*+H W RKGC +Q V* + WQW N+EP* L + F
Sbjct: 182 CWIQLYPPWGQCYTP*VHPGW*RKGCGAQPGFYV*SQGC*WQW*ANSEP*CLQTGPQIGF 361
Query: 67 LQDAVLLLHNNWFLLQYLPG 8
L DA++LL+N+W L Q+ G
Sbjct: 362 LSDALVLLYNHWILFQHNDG 421
Score = 80.3 bits (169), Expect = 2e-015
Identities = 36/74 (48%), Positives = 49/74 (66%)
Frame = +1 / -2
Query: 22 EVETNCCVVTRQHPEEIKAMT*PVDVTAQRLFAIAICYLCLKQRYLIETYIPSFANLHVF 201
E+E+N C+ R+HP+EI+++ V++TAQ L IA L K R L+E +IPSF NL V
Sbjct: 407 EIESNGCIKEREHPKEIQSVAQSVNITAQSLLTIATSNLGFKHRNLVEPHIPSFTNLDVL 228
Query: 202 MVSNIAFAQCGIES 243
MV NIA + IES
Sbjct: 227 MVCNIAPTEGTIES 186
Score = 79.4 bits (167), Expect(2) = 5e-019
Identities = 34/48 (70%), Positives = 36/48 (75%)
Frame = +2 / -3
Query: 104 LSVCSPLPFAIFASNRDI*LRPTSLPLPTCMYSW*VTLPSRNVELNPA 247
L VCSPLP A ASN + L PTSLPLPT MYSW VTLP R V+LNPA
Sbjct: 325 LRVCSPLPLATLASNIETWLSPTSLPLPTWMYSWCVTLPPRRVQLNPA 182
Score = 56.5 bits (117), Expect = 3e-008
Identities = 34/74 (45%), Positives = 41/74 (55%)
Frame = -3 / +1
Query: 242 DSIPHCAKAMLLTMNTCKLAKEGM*VSIKYLCLRQR*QMAMANKR*AVTSTG*VIALISS 63
DSI AML TM+T +L KEGM S ++LCL+ R +AM +K *AV T IS
Sbjct: 187 DSIVPSVGAMLHTMSTSRLVKEGMWGSTRFLCLKPRLLVAMVSKL*AVMFTDWATDWISF 366
Query: 62 GCCLVTTQQLVSTS 21
GC Q L S S
Sbjct: 367 GCSRSFIQPLDSIS 408
Score = 35.0 bits (70), Expect(2) = 0.007
Identities = 14/24 (58%), Positives = 15/24 (62%)
Frame = +3 / -1
Query: 171 HPFLCQLACIHGE*HCLRAMWN*I 242
HPFL Q C HG *HC +N*I
Sbjct: 258 HPFLYQPGCTHGV*HCPHGGYN*I 187
Score = 30.4 bits (60), Expect(2) = 5e-019
Identities = 16/41 (39%), Positives = 19/41 (46%)
Frame = +3 / -1
Query: 6 RPGRY*SRNQLLCSNKTAS*RNQSDDLAGRCHGSAFVRHCH 128
+P *+R Q L AS RN + HGS F HCH
Sbjct: 423 QPSLC*NRIQWLYKRTRASERNPICGPVCKHHGSEFAHHCH 301
Score = 19.9 bits (37), Expect(2) = 0.007
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +2 / -3
Query: 17 VLK*KPIVV**QDSI 61
VLK* P+VV* +SI
Sbjct: 412 VLK*NPMVV*KNESI 368
>gb|BU969103.1|BU969103 HB10J09r BC Hordeum vulgare subsp. vulgare cDNA clone HB10J09
5-PRIME, mRNA sequence
Length = 426
Score = 160 bits (344), Expect = 1e-039
Identities = 63/77 (81%), Positives = 69/77 (89%)
Frame = -2 / +2
Query: 246 AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIANGNGEQTLSRDIYRLGHRFDF 67
AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+LFE K+A GNGEQ LSRDIYRLG FDF
Sbjct: 32 AGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDIYRLGQLFDF 211
Query: 66 FRMLSCYYTTIGFYFST 16
FRMLS Y TT+GFYF T
Sbjct: 212 FRMLSFYVTTVGFYFCT 262
Score = 84.9 bits (179), Expect = 8e-017
Identities = 43/77 (55%), Positives = 46/77 (59%)
Frame = +2 / -1
Query: 17 VLK*KPIVV**QDSILXXXXXXXXXXXXXLSVCSPLPFAIFASNRDI*LRPTSLPLPTCM 196
V K* P VV *+D+IL L CSP P A F SN I*L PTSLPLPT +
Sbjct: 261 VQK*NPTVVT*KDNILKKSKSCPSLYISLLRTCSPFPPATFPSNSAI*LSPTSLPLPTWI 82
Query: 197 YSW*VTLPSRNVELNPA 247
YSW*V P R VELNPA
Sbjct: 81 YSW*VMFP*RRVELNPA 31
Score = 52.8 bits (109), Expect = 4e-007
Identities = 27/76 (35%), Positives = 41/76 (53%)
Frame = +1 / -3
Query: 19 TEVETNCCVVTRQHPEEIKAMT*PVDVTAQRLFAIAICYLCLKQRYLIETYIPSFANLHV 198
TEV+ N + HPE++K + V ++A+ LFA++ K LI+ I SF NL +
Sbjct: 259 TEVKPNSSHIEG*HPEKVKKLPKSVYISAKNLFAVSSRNFSFK*CDLIKPNISSFTNLDI 80
Query: 199 FMVSNIAFAQCGIESC 246
MVS + Q I+ C
Sbjct: 79 LMVSYVPLTQSRIKPC 32
Score = 50.6 bits (104), Expect = 2e-006
Identities = 26/77 (33%), Positives = 43/77 (55%)
Frame = -1 / +1
Query: 247 CRIQFHIARRQCYSP*IHASWQRKGCRSQSNISV*GKDSKWQWRTNAEP*HLPARSSL*F 68
CR+ F+ A R+ SP*++ W RK C + S+ ++* K + RT++ ++ ++ *
Sbjct: 31 CRV*FYSASREHNSP*VYPGW*RKRCWA*SDRTI*RKSCGRKRRTSS*QRYIQTWATF*L 210
Query: 67 LQDAVLLLHNNWFLLQY 17
QD +LL W LL Y
Sbjct: 211 FQDVILLCDYCWVLLLY 261
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 160 bits (344), Expect = 1e-039
Identities = 63/77 (81%), Positives = 69/77 (89%)
Frame = -2 / +3
Query: 246 AGFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIANGNGEQTLSRDIYRLGHRFDF 67
AGFNSTLR+GN+THHEY+QVGKGRDVGLNQI+LFE K+A GNGEQ LSRDIYRLG FDF
Sbjct: 4344 AGFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDIYRLGQLFDF 4523
Query: 66 FRMLSCYYTTIGFYFST 16
FRMLS Y TT+GFYF T
Sbjct: 4524 FRMLSFYVTTVGFYFCT 4574
Score = 84.9 bits (179), Expect = 8e-017
Identities = 43/77 (55%), Positives = 46/77 (59%)
Frame = +2 / -3
Query: 17 VLK*KPIVV**QDSILXXXXXXXXXXXXXLSVCSPLPFAIFASNRDI*LRPTSLPLPTCM 196
V K* P VV *+D+IL L CSP P A F SN I*L PTSLPLPT +
Sbjct: 4573 VQK*NPTVVT*KDNILKKSKSCPSLYISLLRTCSPFPPATFPSNSAI*LSPTSLPLPTWI 4394
Query: 197 YSW*VTLPSRNVELNPA 247
YSW*V P R VELNPA
Sbjct: 4393 YSW*VMFP*RRVELNPA 4343
Score = 52.8 bits (109), Expect = 4e-007
Identities = 27/76 (35%), Positives = 41/76 (53%)
Frame = +1 / -2
Query: 19 TEVETNCCVVTRQHPEEIKAMT*PVDVTAQRLFAIAICYLCLKQRYLIETYIPSFANLHV 198
TEV+ N + HPE++K + V ++A+ LFA++ K LI+ I SF NL +
Sbjct: 4571 TEVKPNSSHIEG*HPEKVKKLPKSVYISAKNLFAVSSRNFSFK*CDLIKPNISSFTNLDI 4392
Query: 199 FMVSNIAFAQCGIESC 246
MVS + Q I+ C
Sbjct: 4391 LMVSYVPLTQSRIKPC 4344
Score = 50.6 bits (104), Expect = 2e-006
Identities = 26/77 (33%), Positives = 43/77 (55%)
Frame = -1 / +2
Query: 247 CRIQFHIARRQCYSP*IHASWQRKGCRSQSNISV*GKDSKWQWRTNAEP*HLPARSSL*F 68
CR+ F+ A R+ SP*++ W RK C + S+ ++* K + RT++ ++ ++ *
Sbjct: 4343 CRV*FYSASREHNSP*VYPGW*RKRCWA*SDRTI*RKSCGRKRRTSS*QRYIQTWATF*L 4522
Query: 67 LQDAVLLLHNNWFLLQY 17
QD +LL W LL Y
Sbjct: 4523 FQDVILLCDYCWVLLLY 4573
>gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd19k01, mRNA
sequence
Length = 644
Score = 158 bits (340), Expect = 5e-039
Identities = 62/76 (81%), Positives = 68/76 (89%)
Frame = -2 / +2
Query: 243 GFNSTLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIANGNGEQTLSRDIYRLGHRFDFF 64
GFNSTLR+GN+THHEY+QVGKGRDVGLNQI+LFE K+A GNGEQ LSRDIYRLG FDFF
Sbjct: 5 GFNSTLRQGNITHHEYIQVGKGRDVGLNQIALFEGKVAGGNGEQVLSRDIYRLGQLFDFF 184
Query: 63 RMLSCYYTTIGFYFST 16
RMLS Y TT+GFYF T
Sbjct: 185 RMLSFYVTTVGFYFCT 232
Score = 83.5 bits (176), Expect = 2e-016
Identities = 42/77 (54%), Positives = 46/77 (59%)
Frame = +2 / -3
Query: 17 VLK*KPIVV**QDSILXXXXXXXXXXXXXLSVCSPLPFAIFASNRDI*LRPTSLPLPTCM 196
V K* P VV *+D+IL L CSP P A F SN I*L PTSLPLPT +
Sbjct: 231 VQK*NPTVVT*KDNILKKSKSCPSLYISLLRTCSPFPPATFPSNSAI*LSPTSLPLPTWI 52
Query: 197 YSW*VTLPSRNVELNPA 247
YSW*V P R VELNP+
Sbjct: 51 YSW*VMFP*RRVELNPS 1
Score = 49.2 bits (101), Expect = 5e-006
Identities = 26/74 (35%), Positives = 40/74 (54%)
Frame = +1 / -2
Query: 19 TEVETNCCVVTRQHPEEIKAMT*PVDVTAQRLFAIAICYLCLKQRYLIETYIPSFANLHV 198
TEV+ N + HPE++K + V ++A+ LFA++ K LI+ I SF NL +
Sbjct: 229 TEVKPNSSHIEG*HPEKVKKLPKSVYISAKNLFAVSSRNFSFK*CDLIKPNISSFTNLDI 50
Query: 199 FMVSNIAFAQCGIE 240
MVS + Q I+
Sbjct: 49 LMVSYVPLTQSRIK 8
>gb|AV932586.1|AV932586 AV932586 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal1e06 5', mRNA sequence
Length = 582
Score = 85.4 bits (180), Expect(2) = 1e-036
Identities = 35/41 (85%), Positives = 37/41 (90%)
Frame = -2 / +3
Query: 234 STLREGNVTHHEYMQVGKGRDVGLNQISLFEAKIANGNGEQ 112
+T + NVTHHEYMQVGKGRDVGLNQISLFEAKIA GNGEQ
Sbjct: 24 NTAXKXNVTHHEYMQVGKGRDVGLNQISLFEAKIAYGNGEQ 146
Score = 83.5 bits (176), Expect(2) = 1e-036
Identities = 32/32 (100%), Positives = 32/32 (100%)
Frame = -2 / +1
Query: 111 TLSRDIYRLGHRFDFFRMLSCYYTTIGFYFST 16
TLSRDIYRLGHRFDFFRMLSCYYTTIGFYFST
Sbjct: 148 TLSRDIYRLGHRFDFFRMLSCYYTTIGFYFST 243
Score = 69.8 bits (146), Expect(2) = 2e-018
Identities = 29/34 (85%), Positives = 31/34 (91%)
Frame = +2 / -3
Query: 113 CSPLPFAIFASNRDI*LRPTSLPLPTCMYSW*VT 214
CSPLP+AIFASN +I*LRPTSLPLPTCMYS VT
Sbjct: 145 CSPLPYAIFASNSEI*LRPTSLPLPTCMYS*CVT 44
Score = 51.9 bits (107), Expect(2) = 8e-013
Identities = 23/39 (58%), Positives = 27/39 (69%)
Frame = +1 / -2
Query: 112 LFAIAICYLCLKQRYLIETYIPSFANLHVFMVSNIAFAQ 228
LFA+ ICYL LK LIET I S ANLHVFM+ N+ +
Sbjct: 146 LFAVTICYLRLK**NLIETNISSLANLHVFMMCNVXLXR 30
Score = 48.7 bits (100), Expect(2) = 2e-014
Identities = 21/40 (52%), Positives = 27/40 (67%)
Frame = -1 / +2
Query: 232 HIARRQCYSP*IHASWQRKGCRSQSNISV*GKDSKWQWRT 113
H + Y+ *IHASWQ K C SQS+ ++*G+DS W RT
Sbjct: 26 HCVXGKRYTS*IHASWQGKRCWSQSDFTI*GEDSIW*RRT 145
Score = 45.5 bits (93), Expect(2) = 2e-014
Identities = 19/31 (61%), Positives = 24/31 (77%)
Frame = -1 / +3
Query: 109 AEP*HLPARSSL*FLQDAVLLLHNNWFLLQY 17
+EP*HLP S *FLQ+ VLLL+ +W LLQ+
Sbjct: 150 SEP*HLPTWPSF*FLQNVVLLLYYHWLLLQH 242
Score = 43.7 bits (89), Expect(2) = 4e-008
Identities = 19/30 (63%), Positives = 20/30 (66%)
Frame = +3 / -1
Query: 114 VRHCHLLSLPQTEIFD*DLHPFLCQLACIH 203
VR H+LS PQ D*D H F CQLACIH
Sbjct: 144 VRRYHMLSSPQIVKSD*DQHLFPCQLACIH 55
Score = 42.8 bits (87), Expect(3) = 1e-009
Identities = 20/36 (55%), Positives = 25/36 (69%)
Frame = -3 / +1
Query: 221 KAMLLTMNTCKLAKEGM*VSIKYLCLRQR*QMAMAN 114
+ L MNTCKLA+E M VSI++ LR+R* M AN
Sbjct: 37 RXTLHIMNTCKLAREEMLVSIRFHYLRRR*HMVTAN 144
Score = 37.7 bits (76), Expect(2) = 2e-018
Identities = 17/29 (58%), Positives = 19/29 (65%)
Frame = +1 / -1
Query: 22 EVETNCCVVTRQHPEEIKAMT*PVDVTAQ 108
EV+ N +V QH EEIK M VDVTAQ
Sbjct: 237 EVKANGSIVAGQHSEEIKTMAKSVDVTAQ 151
Score = 36.8 bits (74), Expect(2) = 8e-013
Identities = 16/29 (55%), Positives = 21/29 (72%)
Frame = +3 / -3
Query: 21 *SRNQLLCSNKTAS*RNQSDDLAGRCHGS 107
*S++Q S++T *RNQ+D GRCHGS
Sbjct: 238 *SKSQW*YSSRTTF*RNQNDGQVGRCHGS 152
Score = 31.8 bits (63), Expect(3) = 1e-009
Identities = 17/29 (58%), Positives = 17/29 (58%)
Frame = -3 / +2
Query: 107 *AVTSTG*VIALISSGCCLVTTQQLVSTS 21
*AVTST I LISS CC T L TS
Sbjct: 152 *AVTSTDLAIVLISSECCPATILPLAFTS 238
Score = 29.0 bits (57), Expect(2) = 4e-008
Identities = 14/16 (87%), Positives = 16/16 (100%)
Frame = +2 / -2
Query: 17 VLK*KPIVV**QDSIL 64
VLK*KP+VV**QD+IL
Sbjct: 242 VLK*KPMVV**QDNIL 195
Score = 18.0 bits (33), Expect(3) = 1e-009
Identities = 7/12 (58%), Positives = 7/12 (58%)
Frame = -2 / +1
Query: 240 FNSTLREGNVTH 205
FNSTLR H
Sbjct: 16 FNSTLRXRXTLH 51
>gb|BI953459.1|BI953459 HVSMEm0012M15f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0012M15f, mRNA sequence
Length = 640
Score = 31.3 bits (62), Expect = 1.1
Identities = 12/44 (27%), Positives = 25/44 (56%)
Frame = +1 / -2
Query: 112 LFAIAICYLCLKQRYLIETYIPSFANLHVFMVSNIAFAQCGIES 243
+F +AIC++CL + ++T A+L F+ + + C +E+
Sbjct: 411 IFGMAICHVCLYHKIPVKTSKNCAASLGXFITTCVTGCTCWLEN 280
>gb|BG416804.1|BG416804 HVSMEk0014J21f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0014J21f, mRNA sequence
Length = 697
Score = 31.3 bits (62), Expect = 1.1
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = -2 / +1
Query: 243 GFNSTLREGNVTHHEYMQVGKGR 175
G N TL + N HE++QV KGR
Sbjct: 457 GVNDTLADSNGKDHEWLQVQKGR 525
>gb|BG369013.2|BG369013 HVSMEi0022E03f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0022E03f, mRNA sequence
Length = 786
Score = 31.3 bits (62), Expect = 1.1
Identities = 11/35 (31%), Positives = 19/35 (54%)
Frame = +1 / +2
Query: 130 CYLCLKQRYLIETYIPSFANLHVFMVSNIAFAQCG 234
CY CL ++ E+ I + +HV ++ + F CG
Sbjct: 170 CYFCLFLLFIAESSIEAVILIHVLLLPYLFFVVCG 274
>gb|AL499868.1|AL499868 AL499868 Hordeum vulgare Barke etiolated leaves Hordeum vulgare
subsp. vulgare cDNA clone HK03N14r 3', mRNA sequence
Length = 626
Score = 30.4 bits (60), Expect = 2.1
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = -1 / +1
Query: 175 GCRSQSNISV*GKDSKWQWRT 113
GCRSQ N + G + K++W+T
Sbjct: 394 GCRSQQNFPLAGPEVKFRWQT 456
>gb|BI960665.1|BI960665 HVSMEn0025K21f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025K21f, mRNA sequence
Length = 657
Score = 30.4 bits (60), Expect = 2.1
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = -1 / +1
Query: 175 GCRSQSNISV*GKDSKWQWRT 113
GCRSQ N + G + K++W+T
Sbjct: 322 GCRSQQNFPLAGPEVKFRWQT 384
>gb|BJ456962.1|BJ456962 BJ456962 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak25g14 3', mRNA sequence
Length = 372
Score = 30.4 bits (60), Expect = 2.1
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = -1 / +1
Query: 175 GCRSQSNISV*GKDSKWQWRT 113
GCRSQ N + G + K++W+T
Sbjct: 274 GCRSQQNFPLAGPEVKFRWQT 336
>gb|BM372458.2|BM372458 EBpi05_SQ003_A09_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_A09 5', mRNA sequence
Length = 534
Score = 30.4 bits (60), Expect = 2.1
Identities = 10/21 (47%), Positives = 15/21 (71%)
Frame = -1 / -3
Query: 175 GCRSQSNISV*GKDSKWQWRT 113
GCRSQ N + G + K++W+T
Sbjct: 238 GCRSQQNFPLAGPEVKFRWQT 176
>gb|CD663955.1|CD663955 UCRHV18_07ah11_b1 Drought-stressed Dicktoo barley epidermis cDNA
library Hordeum vulgare subsp. vulgare cDNA clone
UCRHV18_07ah11, mRNA sequence
Length = 286
Score = 24.9 bits (48), Expect(2) = 2.9
Identities = 12/36 (33%), Positives = 20/36 (55%)
Frame = +2 / -1
Query: 104 LSVCSPLPFAIFASNRDI*LRPTSLPLPTCMYSW*V 211
LSV + L A+ +S D+ T + +CM +W*+
Sbjct: 190 LSVLAVLRTAVVSSQADVLSLVTGVICISCMLAW*I 83
Score = 19.4 bits (36), Expect(2) = 2.9
Identities = 7/16 (43%), Positives = 9/16 (56%)
Frame = +3 / -2
Query: 189 LACIHGE*HCLRAMWN 236
L C HGE + +WN
Sbjct: 105 LVC*HGESGRIVILWN 58
>gb|BF626883.2|BF626883 HVSMEb0001J13f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0001J13f, mRNA sequence
Length = 764
Score = 29.9 bits (59), Expect = 2.9
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = +3 / -1
Query: 66 RNQSDDLAGRCHGSAFVRH 122
R ++LAGR HG A VRH
Sbjct: 143 RGDGEELAGRGHGGAHVRH 87
>gb|BQ755032.1|BQ755032 EBed02_SQ003_I09_R endosperm, 8 DPA, no treatment, cv Optic, EBed02
Hordeum vulgare subsp. vulgare cDNA clone
EBed02_SQ003_I09 5', mRNA sequence
Length = 421
Score = 25.4 bits (49), Expect(2) = 4.3
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2 / +1
Query: 164 RPTSLPLPTCMYSW*VTLPSRN 229
RPTS P P W T P+R+
Sbjct: 163 RPTSCPAPATSRCWSGTSPTRS 228
Score = 18.9 bits (35), Expect(2) = 4.3
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +1 / +3
Query: 1 AAARAGTEVETNCCVVTRQHPEEIKAMT*PVD 96
AAA A + R H + + A+ P+D
Sbjct: 63 AAAMANMQESLTLVGTMRGHNDVVTAIAAPID 158
>gb|BQ754874.1|BQ754874 EBed02_SQ003_B03_R endosperm, 8 DPA, no treatment, cv Optic, EBed02
Hordeum vulgare subsp. vulgare cDNA clone
EBed02_SQ003_B03 5', mRNA sequence
Length = 498
Score = 25.4 bits (49), Expect(2) = 5.0
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2 / +1
Query: 164 RPTSLPLPTCMYSW*VTLPSRN 229
RPTS P P W T P+R+
Sbjct: 151 RPTSCPAPATSRCWSGTSPTRS 216
Score = 18.9 bits (35), Expect(2) = 5.0
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +1 / +3
Query: 1 AAARAGTEVETNCCVVTRQHPEEIKAMT*PVD 96
AAA A + R H + + A+ P+D
Sbjct: 51 AAAMANMQESLTLVGTMRGHNDVVTAIAAPID 146
>gb|BM372510.2|BM372510 EBpi05_SQ003_C18_R pistil, 8 DPA, no treatment, cv Optic, EBpi05
Hordeum vulgare subsp. vulgare cDNA clone
EBpi05_SQ003_C18 5', mRNA sequence
Length = 523
Score = 25.4 bits (49), Expect(2) = 5.3
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2 / +3
Query: 164 RPTSLPLPTCMYSW*VTLPSRN 229
RPTS P P W T P+R+
Sbjct: 177 RPTSCPAPATSRCWSGTSPTRS 242
Score = 18.9 bits (35), Expect(2) = 5.3
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +1 / +2
Query: 1 AAARAGTEVETNCCVVTRQHPEEIKAMT*PVD 96
AAA A + R H + + A+ P+D
Sbjct: 77 AAAMANMQESLTLVGTMRGHNDVVTAIAAPID 172
>gb|AL511638.1|AL511638 AL511638 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY08L14u 3',
mRNA sequence
Length = 531
Score = 25.4 bits (49), Expect(2) = 5.4
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2 / +1
Query: 164 RPTSLPLPTCMYSW*VTLPSRN 229
RPTS P P W T P+R+
Sbjct: 160 RPTSCPAPATSRCWSGTSPTRS 225
Score = 18.9 bits (35), Expect(2) = 5.4
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +1 / +3
Query: 1 AAARAGTEVETNCCVVTRQHPEEIKAMT*PVD 96
AAA A + R H + + A+ P+D
Sbjct: 60 AAAMANMQESLTLVGTMRGHNDVVTAIAAPID 155
>gb|BQ663701.1|BQ663701 HU03K06u HU Hordeum vulgare subsp. vulgare cDNA clone HU03K06
3-PRIME, mRNA sequence
Length = 621
Score = 29.0 bits (57), Expect = 5.5
Identities = 13/44 (29%), Positives = 25/44 (56%)
Frame = +1 / +2
Query: 112 LFAIAICYLCLKQRYLIETYIPSFANLHVFMVSNIAFAQCGIES 243
+F +AIC +CL + ++T S A+L F+ + + C +E+
Sbjct: 467 IFGMAICPVCLFLKIPLKTSKNSAASLGKFITTCVTGCTCWLEN 598
>gb|BQ465877.1|BQ465877 HU04P01r HU Hordeum vulgare subsp. vulgare cDNA clone HU04P01
5-PRIME, mRNA sequence
Length = 542
Score = 25.4 bits (49), Expect(2) = 5.5
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2 / +1
Query: 164 RPTSLPLPTCMYSW*VTLPSRN 229
RPTS P P W T P+R+
Sbjct: 142 RPTSCPAPATSRCWSGTSPTRS 207
Score = 18.9 bits (35), Expect(2) = 5.5
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +1 / +3
Query: 1 AAARAGTEVETNCCVVTRQHPEEIKAMT*PVD 96
AAA A + R H + + A+ P+D
Sbjct: 42 AAAMANMQESLTLVGTMRGHNDVVTAIAAPID 137
>gb|BU996870.1|BU996870 HI06B15r HI Hordeum vulgare subsp. vulgare cDNA clone HI06B15
5-PRIME, mRNA sequence
Length = 576
Score = 25.4 bits (49), Expect(2) = 5.8
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2 / +2
Query: 164 RPTSLPLPTCMYSW*VTLPSRN 229
RPTS P P W T P+R+
Sbjct: 170 RPTSCPAPATSRCWSGTSPTRS 235
Score = 18.9 bits (35), Expect(2) = 5.8
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +1 / +1
Query: 1 AAARAGTEVETNCCVVTRQHPEEIKAMT*PVD 96
AAA A + R H + + A+ P+D
Sbjct: 70 AAAMANMQESLTLVGTMRGHNDVVTAIAAPID 165
>gb|BQ768637.1|BQ768637 EBro08_SQ012_C09_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ012_C09 5', mRNA sequence
Length = 681
Score = 25.4 bits (49), Expect(2) = 6.8
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2 / +3
Query: 164 RPTSLPLPTCMYSW*VTLPSRN 229
RPTS P P W T P+R+
Sbjct: 156 RPTSCPAPATSRCWSGTSPTRS 221
Score = 18.9 bits (35), Expect(2) = 6.8
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +1 / +2
Query: 1 AAARAGTEVETNCCVVTRQHPEEIKAMT*PVD 96
AAA A + R H + + A+ P+D
Sbjct: 56 AAAMANMQESLTLVGTMRGHNDVVTAIAAPID 151
>gb|AL504911.1|AL504911 AL504911 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW06M15V 5', mRNA sequence
Length = 700
Score = 25.4 bits (49), Expect(2) = 6.9
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2 / +2
Query: 164 RPTSLPLPTCMYSW*VTLPSRN 229
RPTS P P W T P+R+
Sbjct: 134 RPTSCPAPATSRCWSGTSPTRS 199
Score = 18.9 bits (35), Expect(2) = 6.9
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +1 / +1
Query: 1 AAARAGTEVETNCCVVTRQHPEEIKAMT*PVD 96
AAA A + R H + + A+ P+D
Sbjct: 34 AAAMANMQESLTLVGTMRGHNDVVTAIAAPID 129
>gb|BJ473108.1|BJ473108 BJ473108 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal38b23 5', mRNA sequence
Length = 598
Score = 28.6 bits (56), Expect = 7.5
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = -2 / +3
Query: 240 FNSTLREGNVTHHEYMQVGKG 178
+NS R+G VTH Y+Q G G
Sbjct: 459 YNSVDRDGTVTHGGYLQHGGG 521
>gb|BG369201.1|BG369201 HVSMEi0023C06f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0023C06f, mRNA sequence
Length = 786
Score = 25.4 bits (49), Expect(2) = 7.7
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = +2 / +3
Query: 164 RPTSLPLPTCMYSW*VTLPSRN 229
RPTS P P W T P+R+
Sbjct: 123 RPTSCPAPATSRCWSGTSPTRS 188
Score = 18.9 bits (35), Expect(2) = 7.7
Identities = 9/32 (28%), Positives = 14/32 (43%)
Frame = +1 / +2
Query: 1 AAARAGTEVETNCCVVTRQHPEEIKAMT*PVD 96
AAA A + R H + + A+ P+D
Sbjct: 23 AAAMANMQESLTLVGTMRGHNDVVTAIAAPID 118
>gb|BG367897.2|BG367897 HVSMEi0014H08f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0014H08f, mRNA sequence
Length = 848
Score = 24.0 bits (46), Expect(2) = 8.3
Identities = 11/34 (32%), Positives = 13/34 (38%)
Frame = -1 / +2
Query: 220 RQCYSP*IHASWQRKGCRSQSNISV*GKDSKWQW 119
R P H W+R S+ V GK W W
Sbjct: 14 RTAADPSPHPIWRRLPAP*PSSHGVRGKTGSWAW 115
Score = 20.3 bits (38), Expect(2) = 8.3
Identities = 6/11 (54%), Positives = 8/11 (72%)
Frame = -3 / +2
Query: 80 IALISSGCCLV 48
+ L SGCC+V
Sbjct: 341 LRLFGSGCCVV 373
>gb|AL507214.1|AL507214 AL507214 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY01D01V 5',
mRNA sequence
Length = 298
Score = 24.9 bits (48), Expect(2) = 9.9
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = +3 / -1
Query: 120 HCHLLSLP 143
HCHLLSLP
Sbjct: 115 HCHLLSLP 92
Score = 17.6 bits (32), Expect(2) = 9.9
Identities = 7/16 (43%), Positives = 9/16 (56%)
Frame = +1 / -2
Query: 37 CCVVTRQHPEEIKAMT 84
CC+ + E KAMT
Sbjct: 294 CCLDASEFSEIGKAMT 247
Database: Hordeum_nucl_with_EST.fasta
Posted date: May 2, 2006 2:38 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 120,240,047
Number of Sequences: 312970
Number of extensions: 1499181
Number of successful extensions: 156438
Number of sequences better than 10.0: 40
length of database: 58,378,179
effective HSP length: 47
effective length of database: 43,668,589
effective search space used: 1484732026
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)