TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCM12h10.yg.2.1
(339 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BE215834.2|BE215834 HV_CEb0008J21f Hordeum vulgare seedl... 145 7e-035
gb|CA031522.1|CA031522 HX10D22r HX Hordeum vulgare subsp. v... 145 7e-035
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 145 7e-035
gb|BJ469639.1|BJ469639 BJ469639 K. Sato unpublished cDNA li... 144 2e-034
gb|BJ473076.1|BJ473076 BJ473076 K. Sato unpublished cDNA li... 144 2e-034
gb|BF616755.1|BF616755 HVSMEc0012I18f Hordeum vulgare seedl... 144 2e-034
gb|BM374437.2|BM374437 EBpi03_SQ003_J24_R pistil, 4 DPA, no... 130 3e-030
gb|BG369637.1|BG369637 HVSMEi0025E14f Hordeum vulgare 20 DA... 119 7e-027
gb|BE559011.2|BE559011 HV_CEb0022E12f Hordeum vulgare seedl... 97 2e-022
>gb|BE215834.2|BE215834 HV_CEb0008J21f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0008J21f, mRNA sequence
Length = 504
Score = 145 bits (312), Expect = 7e-035
Identities = 55/110 (50%), Positives = 74/110 (67%)
Frame = -3 / +1
Query: 331 SVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGI 152
SV TFS+GT++ ILHGGAKYRAT RGFVV+H FAENY LY+R HF+K +E+ +
Sbjct: 160 SVFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHFVKGLEVAL 339
Query: 151 ILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
+L ++ A+ + YI SSWF+ +SW+ AP+ F SGF+W K V
Sbjct: 340 LLVIFLAYGFNNSGAIGYILLSISSWFMALSWLFAPYVFNPSGFEWQKVV 489
>gb|CA031522.1|CA031522 HX10D22r HX Hordeum vulgare subsp. vulgare cDNA clone HX10D22
5-PRIME, mRNA sequence
Length = 478
Score = 145 bits (312), Expect = 7e-035
Identities = 55/110 (50%), Positives = 74/110 (67%)
Frame = -3 / +3
Query: 331 SVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGI 152
SV TFS+GT++ ILHGGAKYRAT RGFVV+H FAENY LY+R HF+K +E+ +
Sbjct: 3 SVFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHFVKGLEVAL 182
Query: 151 ILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
+L ++ A+ + YI SSWF+ +SW+ AP+ F SGF+W K V
Sbjct: 183 LLVIFLAYGFNNSGAIGYILLSISSWFMALSWLFAPYVFNPSGFEWQKVV 332
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 145 bits (312), Expect = 7e-035
Identities = 55/110 (50%), Positives = 74/110 (67%)
Frame = -3 / +3
Query: 331 SVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGI 152
SV TFS+GT++ ILHGGAKYRAT RGFVV+H FAENY LY+R HF+K +E+ +
Sbjct: 4824 SVFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHFVKGLEVAL 5003
Query: 151 ILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
+L ++ A+ + YI SSWF+ +SW+ AP+ F SGF+W K V
Sbjct: 5004 LLVIFLAYGFNNSGAIGYILLSISSWFMALSWLFAPYVFNPSGFEWQKVV 5153
>gb|BJ469639.1|BJ469639 BJ469639 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal27j14 5', mRNA sequence
Length = 604
Score = 144 bits (309), Expect = 2e-034
Identities = 58/111 (52%), Positives = 73/111 (65%)
Frame = -3 / +1
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
+SV TFS+GTK+ +LHGGA+YRAT RGFVV H FAENY LY+R HF+K IEL
Sbjct: 127 ASVFFTFSLGTKTHYYGKTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELM 306
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
I+L V+ + + YI S WF+VV+W+ APF F SGF+W K V
Sbjct: 307 ILLIVFEIFGQSYRGAIAYIFITFSMWFMVVTWLFAPFLFNPSGFEWQKIV 459
>gb|BJ473076.1|BJ473076 BJ473076 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal40n15 5', mRNA sequence
Length = 614
Score = 144 bits (309), Expect = 2e-034
Identities = 58/111 (52%), Positives = 73/111 (65%)
Frame = -3 / +3
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
+SV TFS+GTK+ +LHGGA+YRAT RGFVV H FAENY LY+R HF+K IEL
Sbjct: 138 ASVFFTFSLGTKTHYYGKTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELM 317
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
I+L V+ + + YI S WF+VV+W+ APF F SGF+W K V
Sbjct: 318 ILLIVFEIFGQSYRGAIAYIFITFSMWFMVVTWLFAPFLFNPSGFEWQKIV 470
>gb|BF616755.1|BF616755 HVSMEc0012I18f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0012I18f, mRNA sequence
Length = 762
Score = 144 bits (308), Expect = 2e-034
Identities = 58/111 (52%), Positives = 73/111 (65%)
Frame = -3 / +3
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
+SV TFS+GTK+ +LHGGA+YRAT RGFVV H FAENY LY+R HF+K IEL
Sbjct: 21 ASVLFTFSLGTKTHWYGKTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELM 200
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
I+L V+ + + YI S WF+VV+W+ APF F SGF+W K V
Sbjct: 201 ILLIVFEIFGQSYRGAIAYIFITFSMWFMVVTWLFAPFLFNPSGFEWQKIV 353
>gb|BM374437.2|BM374437 EBpi03_SQ003_J24_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ003_J24 5', mRNA sequence
Length = 573
Score = 130 bits (278), Expect = 3e-030
Identities = 53/103 (51%), Positives = 67/103 (65%)
Frame = -3 / +1
Query: 334 SSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELG 155
+SV TFS+GTK+ +LHGGA+YRAT RGFVV H FAENY LY+R HF+K IEL
Sbjct: 265 ASVFFTFSLGTKTHYYGKTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELM 444
Query: 154 IILTVYAAHSVIAKNTLVYIXXXXSSWFLVVSWIMAPFAFXXS 26
I+L V+ + + YI S WF+VV+W+ APF F S
Sbjct: 445 ILLIVFEIFGQSYRGAIAYIFITFSMWFMVVTWLFAPFLFNPS 573
>gb|BG369637.1|BG369637 HVSMEi0025E14f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0025E14f, mRNA sequence
Length = 725
Score = 119 bits (254), Expect = 7e-027
Identities = 47/91 (51%), Positives = 58/91 (63%)
Frame = -3 / +2
Query: 274 LHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIELGIILTVYAAHSVIAKNTLVYI 95
LHGGA+YRAT RGF+ H FAENY LY+R HF+K IEL I+L V+ + + YI
Sbjct: 2 LHGGAEYRATGRGFLGFHAKFAENYRLYSRSHFVKGIELMILLIVFEIFRQSYRGAIAYI 181
Query: 94 XXXXSSWFLVVSWIMAPFAFXXSGFDWLKTV 2
S WF+V +W+ APF F SGF W K V
Sbjct: 182 FITFSMWFMVATWLFAPFLFNPSGFQWQKIV 274
>gb|BE559011.2|BE559011 HV_CEb0022E12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0022E12f, mRNA sequence
Length = 730
Score = 97.3 bits (206), Expect(2) = 2e-022
Identities = 37/67 (55%), Positives = 50/67 (74%)
Frame = -3 / +2
Query: 337 FSSVXXTFSMGTKSXXXDXXILHGGAKYRATXRGFVVQHKSFAENYXLYARGHFIKAIEL 158
F S+ TFS+GT++ ILHGGAK+RAT RGFVV+H FA+NY LY+R HF+KA+E+
Sbjct: 104 FCSLFFTFSLGTRTHYFGRTILHGGAKHRATGRGFVVRHIKFADNYRLYSRSHFVKALEV 283
Query: 157 GIILTVY 137
++L Y
Sbjct: 284 ALLLIAY 304
Score = 26.3 bits (51), Expect(2) = 2e-022
Identities = 7/16 (43%), Positives = 11/16 (68%)
Frame = -3 / +1
Query: 82 SSWFLVVSWIMAPFAF 35
++WF V+ W AP+ F
Sbjct: 361 NTWFKVIYWTFAPYIF 408
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 121,417,268
Number of Sequences: 312970
Number of extensions: 1240709
Number of successful extensions: 76773
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 49
effective length of database: 43,042,649
effective search space used: 2711686887
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)