TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCL28h07.yg.2.1
(277 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ469639.1|BJ469639 BJ469639 K. Sato unpublished cDNA li... 198 9e-051
gb|BJ473076.1|BJ473076 BJ473076 K. Sato unpublished cDNA li... 198 9e-051
gb|BM374437.2|BM374437 EBpi03_SQ003_J24_R pistil, 4 DPA, no... 198 9e-051
gb|BF616755.1|BF616755 HVSMEc0012I18f Hordeum vulgare seedl... 191 1e-048
gb|BE215834.2|BE215834 HV_CEb0008J21f Hordeum vulgare seedl... 151 1e-036
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 151 1e-036
gb|CA031522.1|CA031522 HX10D22r HX Hordeum vulgare subsp. v... 141 8e-034
gb|BE559011.2|BE559011 HV_CEb0022E12f Hordeum vulgare seedl... 138 1e-032
gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA li... 130 2e-030
gb|BG369637.1|BG369637 HVSMEi0025E14f Hordeum vulgare 20 DA... 129 3e-030
gb|BU982241.1|BU982241 HA25P01r HA Hordeum vulgare subsp. v... 124 1e-028
gb|BU971000.1|BU971000 HB16F02r BC Hordeum vulgare subsp. v... 109 4e-024
>gb|BJ469639.1|BJ469639 BJ469639 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal27j14 5', mRNA sequence
Length = 604
Score = 198 bits (426), Expect = 9e-051
Identities = 83/85 (97%), Positives = 84/85 (98%)
Frame = -1 / +1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
VLMQLQLASVFFTFSLGTKTHYYG TLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF
Sbjct: 106 VLMQLQLASVFFTFSLGTKTHYYGKTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 285
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIELMILLIV+EIFGQSYRGAIA
Sbjct: 286 VKGIELMILLIVFEIFGQSYRGAIA 360
Score = 116 bits (247), Expect(2) = 2e-033
Identities = 46/52 (88%), Positives = 49/52 (94%)
Frame = +3 / -3
Query: 3 AIAPRYDCPNISYTISKIISSMPFTKWLREYNR*FSANLAWNTTNPRPVALY 158
A+APRYDCP IS TISKII+SMP TKWLREY+R*FSANLAWNTTNPRPVALY
Sbjct: 359 AMAPRYDCPKISNTISKIINSMPLTKWLREYSR*FSANLAWNTTNPRPVALY 204
Score = 113 bits (241), Expect = 3e-025
Identities = 50/80 (62%), Positives = 63/80 (78%)
Frame = +1 / -1
Query: 1 TQ*LLGMTAQIFHTQ*AKSSVQCPSQNGFANTIDNSQQTWHGTPQIHAL*LYIQLLHVAA 180
T+ LLG TA+ T+*A+SS +CP +NG A+T D+S++TWHGTP+IH *LY QLLHVAA
Sbjct: 361 TRWLLGTTARRSQTR*ARSSTRCP*RNGSASTADSSRRTWHGTPRIHVQ*LYTQLLHVAA 182
Query: 181 WFRSSEFWSQERM*RTQMLT 240
FRSSE W + RM*R +MLT
Sbjct: 181 SFRSSESWFRGRM*RRRMLT 122
Score = 109 bits (232), Expect = 5e-024
Identities = 50/78 (64%), Positives = 60/78 (76%)
Frame = -3 / +3
Query: 239 VSICVLHILSWDQNSLLRNHAATWRS*I*SYRAWICGVPCQVC*ELSIVFAKPFCEGH*T 60
VSI +LHIL +Q+SLLR AATWRS*+*SY WI GVPCQV ELS V A+PF +GH
Sbjct: 123 VSIRLLHILPRNQDSLLRKDAATWRS*V*SYWTWIRGVPCQVRRELSAVLAEPFRQGHRV 302
Query: 59 DDFAYCV*NIWAVIPRSY 6
DD AY V*++ AV+PRS+
Sbjct: 303 DDLAYRV*DLRAVVPRSH 356
Score = 43.2 bits (88), Expect(2) = 2e-033
Identities = 19/21 (90%), Positives = 19/21 (90%)
Frame = +3 / -3
Query: 195 *VLVPRENVKNTDANWSCIST 257
*VLVPRENVK TDAN SCIST
Sbjct: 167 *VLVPRENVKKTDAN*SCIST 105
>gb|BJ473076.1|BJ473076 BJ473076 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal40n15 5', mRNA sequence
Length = 614
Score = 198 bits (426), Expect = 9e-051
Identities = 83/85 (97%), Positives = 84/85 (98%)
Frame = -1 / +3
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
VLMQLQLASVFFTFSLGTKTHYYG TLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF
Sbjct: 117 VLMQLQLASVFFTFSLGTKTHYYGKTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 296
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIELMILLIV+EIFGQSYRGAIA
Sbjct: 297 VKGIELMILLIVFEIFGQSYRGAIA 371
Score = 116 bits (247), Expect(2) = 2e-033
Identities = 46/52 (88%), Positives = 49/52 (94%)
Frame = +3 / -2
Query: 3 AIAPRYDCPNISYTISKIISSMPFTKWLREYNR*FSANLAWNTTNPRPVALY 158
A+APRYDCP IS TISKII+SMP TKWLREY+R*FSANLAWNTTNPRPVALY
Sbjct: 370 AMAPRYDCPKISNTISKIINSMPLTKWLREYSR*FSANLAWNTTNPRPVALY 215
Score = 113 bits (241), Expect = 3e-025
Identities = 50/80 (62%), Positives = 63/80 (78%)
Frame = +1 / -3
Query: 1 TQ*LLGMTAQIFHTQ*AKSSVQCPSQNGFANTIDNSQQTWHGTPQIHAL*LYIQLLHVAA 180
T+ LLG TA+ T+*A+SS +CP +NG A+T D+S++TWHGTP+IH *LY QLLHVAA
Sbjct: 372 TRWLLGTTARRSQTR*ARSSTRCP*RNGSASTADSSRRTWHGTPRIHVQ*LYTQLLHVAA 193
Query: 181 WFRSSEFWSQERM*RTQMLT 240
FRSSE W + RM*R +MLT
Sbjct: 192 SFRSSESWFRGRM*RRRMLT 133
Score = 109 bits (232), Expect = 5e-024
Identities = 50/78 (64%), Positives = 60/78 (76%)
Frame = -3 / +2
Query: 239 VSICVLHILSWDQNSLLRNHAATWRS*I*SYRAWICGVPCQVC*ELSIVFAKPFCEGH*T 60
VSI +LHIL +Q+SLLR AATWRS*+*SY WI GVPCQV ELS V A+PF +GH
Sbjct: 134 VSIRLLHILPRNQDSLLRKDAATWRS*V*SYWTWIRGVPCQVRRELSAVLAEPFRQGHRV 313
Query: 59 DDFAYCV*NIWAVIPRSY 6
DD AY V*++ AV+PRS+
Sbjct: 314 DDLAYRV*DLRAVVPRSH 367
Score = 43.2 bits (88), Expect(2) = 2e-033
Identities = 19/21 (90%), Positives = 19/21 (90%)
Frame = +3 / -2
Query: 195 *VLVPRENVKNTDANWSCIST 257
*VLVPRENVK TDAN SCIST
Sbjct: 178 *VLVPRENVKKTDAN*SCIST 116
>gb|BM374437.2|BM374437 EBpi03_SQ003_J24_R pistil, 4 DPA, no treatment, cv Optic, EBpi03
Hordeum vulgare subsp. vulgare cDNA clone
EBpi03_SQ003_J24 5', mRNA sequence
Length = 573
Score = 198 bits (426), Expect = 9e-051
Identities = 83/85 (97%), Positives = 84/85 (98%)
Frame = -1 / +1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
VLMQLQLASVFFTFSLGTKTHYYG TLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF
Sbjct: 244 VLMQLQLASVFFTFSLGTKTHYYGKTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 423
Query: 76 VKGIELMILLIVYEIFGQSYRGAIA 2
VKGIELMILLIV+EIFGQSYRGAIA
Sbjct: 424 VKGIELMILLIVFEIFGQSYRGAIA 498
Score = 116 bits (247), Expect(2) = 1e-033
Identities = 46/52 (88%), Positives = 49/52 (94%)
Frame = +3 / -2
Query: 3 AIAPRYDCPNISYTISKIISSMPFTKWLREYNR*FSANLAWNTTNPRPVALY 158
A+APRYDCP IS TISKII+SMP TKWLREY+R*FSANLAWNTTNPRPVALY
Sbjct: 497 AMAPRYDCPKISNTISKIINSMPLTKWLREYSR*FSANLAWNTTNPRPVALY 342
Score = 113 bits (241), Expect = 3e-025
Identities = 50/80 (62%), Positives = 63/80 (78%)
Frame = +1 / -3
Query: 1 TQ*LLGMTAQIFHTQ*AKSSVQCPSQNGFANTIDNSQQTWHGTPQIHAL*LYIQLLHVAA 180
T+ LLG TA+ T+*A+SS +CP +NG A+T D+S++TWHGTP+IH *LY QLLHVAA
Sbjct: 499 TRWLLGTTARRSQTR*ARSSTRCP*RNGSASTADSSRRTWHGTPRIHVQ*LYTQLLHVAA 320
Query: 181 WFRSSEFWSQERM*RTQMLT 240
FRSSE W + RM*R +MLT
Sbjct: 319 SFRSSESWFRGRM*RRRMLT 260
Score = 109 bits (232), Expect = 5e-024
Identities = 50/78 (64%), Positives = 60/78 (76%)
Frame = -3 / +3
Query: 239 VSICVLHILSWDQNSLLRNHAATWRS*I*SYRAWICGVPCQVC*ELSIVFAKPFCEGH*T 60
VSI +LHIL +Q+SLLR AATWRS*+*SY WI GVPCQV ELS V A+PF +GH
Sbjct: 261 VSIRLLHILPRNQDSLLRKDAATWRS*V*SYWTWIRGVPCQVRRELSAVLAEPFRQGHRV 440
Query: 59 DDFAYCV*NIWAVIPRSY 6
DD AY V*++ AV+PRS+
Sbjct: 441 DDLAYRV*DLRAVVPRSH 494
Score = 43.2 bits (88), Expect(2) = 1e-033
Identities = 19/21 (90%), Positives = 19/21 (90%)
Frame = +3 / -2
Query: 195 *VLVPRENVKNTDANWSCIST 257
*VLVPRENVK TDAN SCIST
Sbjct: 305 *VLVPRENVKKTDAN*SCIST 243
>gb|BF616755.1|BF616755 HVSMEc0012I18f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0012I18f, mRNA sequence
Length = 762
Score = 191 bits (411), Expect = 1e-048
Identities = 80/84 (95%), Positives = 82/84 (97%)
Frame = -1 / +3
Query: 253 LMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFV 74
LMQLQLASV FTFSLGTKTH+YG TLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFV
Sbjct: 3 LMQLQLASVLFTFSLGTKTHWYGKTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFV 182
Query: 73 KGIELMILLIVYEIFGQSYRGAIA 2
KGIELMILLIV+EIFGQSYRGAIA
Sbjct: 183 KGIELMILLIVFEIFGQSYRGAIA 254
Score = 146 bits (313), Expect = 3e-035
Identities = 63/84 (75%), Positives = 66/84 (78%)
Frame = +3 / -3
Query: 3 AIAPRYDCPNISYTISKIISSMPFTKWLREYNR*FSANLAWNTTNPRPVALYXXXXXXXX 182
A+APRYDCP IS TISKII+SMP TKWLREY+R*FSANLAWNTTNPRPVALY
Sbjct: 253 AMAPRYDCPKISNTISKIINSMPLTKWLREYSR*FSANLAWNTTNPRPVALYSAPPCSSV 74
Query: 183 XXXX*VLVPRENVKNTDANWSCIS 254
*VLVPRENV TDAN SCIS
Sbjct: 73 FPYQ*VLVPRENVNKTDAN*SCIS 2
Score = 109 bits (232), Expect = 5e-024
Identities = 48/80 (60%), Positives = 62/80 (77%)
Frame = +1 / -1
Query: 1 TQ*LLGMTAQIFHTQ*AKSSVQCPSQNGFANTIDNSQQTWHGTPQIHAL*LYIQLLHVAA 180
T+ LLG TA+ T+*A+SS +CP +NG A+T D+S++TWHGTP+IH *LY QLLHVAA
Sbjct: 255 TRWLLGTTARRSQTR*ARSSTRCP*RNGSASTADSSRRTWHGTPRIHVQ*LYTQLLHVAA 76
Query: 181 WFRSSEFWSQERM*RTQMLT 240
FR+SE W + RM* +MLT
Sbjct: 75 SFRTSESWFRGRM*TRRMLT 16
Score = 106 bits (226), Expect = 3e-023
Identities = 48/78 (61%), Positives = 60/78 (76%)
Frame = -3 / +2
Query: 239 VSICVLHILSWDQNSLLRNHAATWRS*I*SYRAWICGVPCQVC*ELSIVFAKPFCEGH*T 60
VSI ++HIL +Q+SL+R AATWRS*+*SY WI GVPCQV ELS V A+PF +GH
Sbjct: 17 VSIRLVHILPRNQDSLVRKDAATWRS*V*SYWTWIRGVPCQVRRELSAVLAEPFRQGHRV 196
Query: 59 DDFAYCV*NIWAVIPRSY 6
DD AY V*++ AV+PRS+
Sbjct: 197 DDLAYRV*DLRAVVPRSH 250
>gb|BE215834.2|BE215834 HV_CEb0008J21f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0008J21f, mRNA sequence
Length = 504
Score = 151 bits (324), Expect = 1e-036
Identities = 59/84 (70%), Positives = 72/84 (85%)
Frame = -1 / +1
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+ MQ QL SVFFTFSLGT+THY+G T+LHGGA+YRATGRGFVV H KFAENYRLYSRSHF
Sbjct: 136 ITMQFQLCSVFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHF 315
Query: 76 VKGIELMILLIVYEIFGQSYRGAI 5
VKG+E+ +LL+++ +G + GAI
Sbjct: 316 VKGLEVALLLVIFLAYGFNNSGAI 387
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 151 bits (324), Expect = 1e-036
Identities = 59/84 (70%), Positives = 72/84 (85%)
Frame = -1 / +3
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+ MQ QL SVFFTFSLGT+THY+G T+LHGGA+YRATGRGFVV H KFAENYRLYSRSHF
Sbjct: 4800 ITMQFQLCSVFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHF 4979
Query: 76 VKGIELMILLIVYEIFGQSYRGAI 5
VKG+E+ +LL+++ +G + GAI
Sbjct: 4980 VKGLEVALLLVIFLAYGFNNSGAI 5051
>gb|CA031522.1|CA031522 HX10D22r HX Hordeum vulgare subsp. vulgare cDNA clone HX10D22
5-PRIME, mRNA sequence
Length = 478
Score = 141 bits (303), Expect = 8e-034
Identities = 55/76 (72%), Positives = 67/76 (88%)
Frame = -1 / +3
Query: 232 SVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELMI 53
SVFFTFSLGT+THY+G T+LHGGA+YRATGRGFVV H KFAENYRLYSRSHFVKG+E+ +
Sbjct: 3 SVFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHFVKGLEVAL 182
Query: 52 LLIVYEIFGQSYRGAI 5
LL+++ +G + GAI
Sbjct: 183 LLVIFLAYGFNNSGAI 230
>gb|BE559011.2|BE559011 HV_CEb0022E12f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0022E12f, mRNA sequence
Length = 730
Score = 138 bits (295), Expect = 1e-032
Identities = 53/73 (72%), Positives = 64/73 (87%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+ MQLQ S+FFTFSLGT+THY+G T+LHGGA++RATGRGFVV H KFA+NYRLYSRSHF
Sbjct: 86 ITMQLQFCSLFFTFSLGTRTHYFGRTILHGGAKHRATGRGFVVRHIKFADNYRLYSRSHF 265
Query: 76 VKGIELMILLIVY 38
VK +E+ +LLI Y
Sbjct: 266 VKALEVALLLIAY 304
>gb|AV836635.1|AV836635 AV836635 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd19k01, mRNA
sequence
Length = 644
Score = 130 bits (278), Expect = 2e-030
Identities = 51/62 (82%), Positives = 56/62 (90%)
Frame = -1 / +2
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRSHF 77
+ MQ QL SVFFTFSLGT+THY+G T+LHGGA+YRATGRGFVV H KFAENYRLYSRSHF
Sbjct: 458 ITMQFQLCSVFFTFSLGTRTHYFGRTILHGGAKYRATGRGFVVRHIKFAENYRLYSRSHF 637
Query: 76 VK 71
VK
Sbjct: 638 VK 643
>gb|BG369637.1|BG369637 HVSMEi0025E14f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0025E14f, mRNA sequence
Length = 725
Score = 129 bits (277), Expect = 3e-030
Identities = 54/58 (93%), Positives = 56/58 (96%)
Frame = -1 / +2
Query: 175 LHGGAEYRATGRGFVVFHAKFAENYRLYSRSHFVKGIELMILLIVYEIFGQSYRGAIA 2
LHGGAEYRATGRGF+ FHAKFAENYRLYSRSHFVKGIELMILLIV+EIF QSYRGAIA
Sbjct: 2 LHGGAEYRATGRGFLGFHAKFAENYRLYSRSHFVKGIELMILLIVFEIFRQSYRGAIA 175
Score = 106 bits (226), Expect = 3e-023
Identities = 43/52 (82%), Positives = 46/52 (88%)
Frame = +3 / -3
Query: 3 AIAPRYDCPNISYTISKIISSMPFTKWLREYNR*FSANLAWNTTNPRPVALY 158
A+APRYDC IS TISKII+SMP TKWLREY+R*FSANLAWN NPRPVALY
Sbjct: 174 AMAPRYDCRKISNTISKIINSMPLTKWLREYSR*FSANLAWNPRNPRPVALY 19
>gb|BU982241.1|BU982241 HA25P01r HA Hordeum vulgare subsp. vulgare cDNA clone HA25P01
5-PRIME, mRNA sequence
Length = 300
Score = 124 bits (265), Expect = 1e-028
Identities = 47/58 (81%), Positives = 54/58 (93%)
Frame = -1 / +3
Query: 256 VLMQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSRS 83
++MQLQLASVFFTF LGTKTHYYG T+LHGGA+YR TGRGFVV+HAKFA+NY +YSRS
Sbjct: 126 IIMQLQLASVFFTFQLGTKTHYYGRTILHGGAKYRPTGRGFVVYHAKFADNYHMYSRS 299
>gb|BU971000.1|BU971000 HB16F02r BC Hordeum vulgare subsp. vulgare cDNA clone HB16F02
5-PRIME, mRNA sequence
Length = 380
Score = 109 bits (233), Expect = 4e-024
Identities = 41/55 (74%), Positives = 49/55 (89%)
Frame = -1 / +3
Query: 250 MQLQLASVFFTFSLGTKTHYYGTTLLHGGAEYRATGRGFVVFHAKFAENYRLYSR 86
MQ+ +SVF+TFS+GTK+HYYG T+LHGGA+YRATGRGFVV H FAENYRLY+R
Sbjct: 216 MQMNFSSVFYTFSMGTKSHYYGRTILHGGAKYRATGRGFVVQHKSFAENYRLYAR 380
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 121,280,438
Number of Sequences: 312970
Number of extensions: 1452849
Number of successful extensions: 121674
Number of sequences better than 1.0e-020: 13
length of database: 58,378,179
effective HSP length: 48
effective length of database: 43,355,619
effective search space used: 1864291617
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)