TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH2g03.yg.2.1
(537 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 94 2e-034
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 94.1 bits (199), Expect(2) = 2e-034
Identities = 38/56 (67%), Positives = 43/56 (76%)
Frame = +3 / +3
Query: 369 DQVIYKIKLPGNAILXEGKPENQXHAIIFTRXXXXQTIDMNQEHYMEEXLKMXNLL 536
D+ IY +KLPGN L EGKPENQ HA+IFTR QTIDMNQ++Y EE LKM NLL
Sbjct: 3909 DKEIYSVKLPGNPKLGEGKPENQNHAVIFTRGNAVQTIDMNQDNYFEEALKMRNLL 4076
Score = 71.2 bits (149), Expect(2) = 2e-034
Identities = 29/53 (54%), Positives = 39/53 (73%)
Frame = +3 / +3
Query: 114 QCKAIADMKFTYVVSCQLYGIQKRSNEPCAHDILRLMTEYPSLRVAYIDEVEA 272
+ +A AD+KFTYVV+CQ+YG+QK +P A DI LM +LR+AYID VE+
Sbjct: 3684 EARAQADLKFTYVVTCQIYGVQKGEGKPEAADIALLMQRNEALRIAYIDVVES 3842
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 178,174,731
Number of Sequences: 312970
Number of extensions: 1990118
Number of successful extensions: 19704
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5469398912
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)