TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH22b04.yg.2.1
(457 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CA030098.1|CA030098 HX06B10r HX Hordeum vulgare subsp. v... 308 8e-084
gb|CK125346.1|CK125346 BES1824107m01 BES1824 Hordeum vulgar... 262 7e-070
gb|AV833557.1|AV833557 AV833557 K. Sato unpublished cDNA li... 244 2e-064
gb|BU987426.1|BU987426 HF14L16r HF Hordeum vulgare subsp. v... 244 2e-064
gb|BU983633.1|BU983633 HA30G17r HA Hordeum vulgare subsp. v... 216 4e-064
gb|BJ472879.1|BJ472879 BJ472879 K. Sato unpublished cDNA li... 188 2e-047
gb|BJ465518.1|BJ465518 BJ465518 K. Sato unpublished cDNA li... 184 2e-046
gb|BQ467802.1|BQ467802 HS04P07r HS Hordeum vulgare subsp. v... 167 3e-041
gb|CA004277.1|CA004277 HS17B22r HS Hordeum vulgare subsp. v... 164 2e-040
gb|BU973765.1|BU973765 HB25O11r BC Hordeum vulgare subsp. v... 159 1e-038
gb|CA021581.1|CA021581 HZ40I04r HZ Hordeum vulgare subsp. v... 132 1e-030
gb|BQ472656.1|BQ472656 HB01O12r BC Hordeum vulgare subsp. v... 66 2e-024
gb|CA014505.1|CA014505 HT11I16r HT Hordeum vulgare subsp. v... 62 1e-022
gb|BU988941.1|BU988941 HF19D14r HF Hordeum vulgare subsp. v... 66 2e-022
gb|BG365974.2|BG365974 HVSMEi0005I24f Hordeum vulgare 20 DA... 70 3e-021
gb|BG367473.2|BG367473 HVSMEi0012G07f Hordeum vulgare 20 DA... 69 8e-021
>gb|CA030098.1|CA030098 HX06B10r HX Hordeum vulgare subsp. vulgare cDNA clone HX06B10
5-PRIME, mRNA sequence
Length = 639
Score = 308 bits (668), Expect = 8e-084
Identities = 124/152 (81%), Positives = 141/152 (92%)
Frame = +1 / +2
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
T+SYFAMKVMDK SL RKKLLRAQTE+EILQ LDHPFLPTLYTHFETDKFSCLVMEFCP
Sbjct: 116 TKSYFAMKVMDKASLTGRKKLLRAQTEKEILQCLDHPFLPTLYTHFETDKFSCLVMEFCP 295
Query: 181 GGDLHTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIML 360
GGDLHTLRQ+Q GKYF EQA KFYVAE+LLA+EYLHMLGIIYRDLKPEN+LVR+DGHIML
Sbjct: 296 GGDLHTLRQKQRGKYFPEQAVKFYVAEILLAMEYLHMLGIIYRDLKPENILVRDDGHIML 475
Query: 361 SDFDLSLRCSVSLTVIKSANPGLDAMQRNNAA 456
SDFDLSLRC+VS T+I+S+NP +A+++++ A
Sbjct: 476 SDFDLSLRCTVSPTLIRSSNPETEALRKSSQA 571
Score = 188 bits (404), Expect = 2e-047
Identities = 89/143 (62%), Positives = 98/143 (68%)
Frame = -3 / -1
Query: 434 ASRPGFADLITVRLTEQRRDRSKSDSMMWPSSRTRTFSGLRSRYIIPSICRYSNARSTSA 255
AS G DLI V LT QRR+RSKSDSMM PSSRTR FSG RSRY+IPS+C+YS AR SA
Sbjct: 549 ASVSGLDDLIRVGLTVQRRERSKSDSMMCPSSRTRMFSGFRSRYMIPSMCKYSIARRISA 370
Query: 254 T*NFAACSEKYFPGCLWRRV*RSPPGQNSITKHENLSVSK*VYRVGRNGWSRDCXXXXXX 75
T*N ACS KY P C V +SPPGQNSIT+HENLSVSK VY VGR G S+ C
Sbjct: 369 T*NLTACSGKYLPRCFCLSVCKSPPGQNSITRHENLSVSKCVYNVGRKG*SKHCKISFSV 190
Query: 74 XXXXXXXRLAKEVLSITFMAK*L 6
R + LSITF+AK*L
Sbjct: 189 WARSNFLRPVSDALSITFIAK*L 121
Score = 112 bits (239), Expect = 1e-024
Identities = 52/126 (41%), Positives = 78/126 (61%)
Frame = -2 / -3
Query: 387 TAKR*IEVRQHDVAIFPNEDILWFKITVYNPKHMQVFQCKEHLSYIELCCLL*KIFSRLP 208
TAK ++V HDV + ++++ +IT+Y+ +H+QV KE Y+E L KI + L
Sbjct: 502 TAKGEVKVG*HDVPVITDKNVFRLQITIYDTEHVQVLHSKEDFCYVEFNSLFWKILATLF 323
Query: 207 LAKSVKIPSRAEFHNQA*KLVCLKVSIQGW*KWMVQGLQDLPLGLSSKQLLPTCQRSLIH 28
L+K +++ RA+ HNQ +LV LKV +Q + M+Q L D L L SKQLL C+ L+H
Sbjct: 322 LSKCMQVSPRAKLHNQTRELVSLKVCVQCRKERMIQAL*DFLLSLGSKQLLTACK*CLVH 143
Query: 27 NLHGKV 10
NLH K+
Sbjct: 142 NLHCKI 125
Score = 87.2 bits (184), Expect(2) = 2e-021
Identities = 43/103 (41%), Positives = 62/103 (60%)
Frame = +3 / +1
Query: 6 ELLCHEGYG*DFFGKSEEAASSSDRAGDPAVPGPSISTNPVYSL*DRQVFMLGYGILPWR 185
EL C+EGYG + E ASS D G+ V G S ++ V++L*D QV + GYG+LPW
Sbjct: 121 ELFCNEGYGQGITYRP*EVASSPD*EGNLTVLGSSFPSDIVHTL*D*QVLVSGYGVLPWG 300
Query: 186 GSSHSSPKAAWKIFFRASSKVLCS*GAPCIGIPAYAWDYIP*S 314
+++ + AW++F R S +L S PC G+ A+A +I *S
Sbjct: 301 RLAYT*TETAWQVFSRTSC*ILRSRNPPCYGVLAHARYHIS*S 429
Score = 34.1 bits (68), Expect(2) = 2e-021
Identities = 15/33 (45%), Positives = 22/33 (66%)
Frame = +3 / +1
Query: 351 HHAVGLRSISSLFSEPNGDQVRKSWPRCNAEEQ 449
HHA+ L + SL+S+P+ +QV S R +EEQ
Sbjct: 466 HHAIRL*PLPSLYSQPDSNQVI*SRNRSTSEEQ 564
>gb|CK125346.1|CK125346 BES1824107m01 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010M017 5-PRIME, mRNA sequence
Length = 884
Score = 262 bits (567), Expect = 7e-070
Identities = 108/144 (75%), Positives = 124/144 (86%)
Frame = +1 / +1
Query: 13 FAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCPGGDL 192
FA+KVMD L SRKK+LRAQTEREILQ LDHPFLPTLY++F TD SCLVME+CPGGDL
Sbjct: 325 FALKVMDIEYLISRKKMLRAQTEREILQMLDHPFLPTLYSYFTTDNLSCLVMEYCPGGDL 504
Query: 193 HTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFD 372
H LRQ+QP + FSE AA+FYVAEVLLALEYLHMLG+IYRDLKPEN+LVREDGHIMLSDFD
Sbjct: 505 HVLRQKQPTRCFSEAAARFYVAEVLLALEYLHMLGVIYRDLKPENILVREDGHIMLSDFD 684
Query: 373 LSLRCSVSLTVIKSANPGLDAMQR 444
LSLRCSV+ +++ ++ G D R
Sbjct: 685 LSLRCSVNPMLVRCSSVGRDEPPR 756
Score = 171 bits (369), Expect = 1e-042
Identities = 82/140 (58%), Positives = 92/140 (65%)
Frame = -3 / -1
Query: 434 ASRPGFADLITVRLTEQRRDRSKSDSMMWPSSRTRTFSGLRSRYIIPSICRYSNARSTSA 255
+S P L ++ LTE RDRSKS+SMM PSSRT FSG RSRYI P+ICRYSNAR TSA
Sbjct: 746 SSLPTDEHLTSMGLTEHLRDRSKSESMMCPSSRTSMFSGFRSRYITPNICRYSNARRTSA 567
Query: 254 T*NFAACSEKYFPGCLWRRV*RSPPGQNSITKHENLSVSK*VYRVGRNGWSRDCXXXXXX 75
T*N AA SEK+ GC R RSPPGQ SIT+ + LSV K* YRVGRNGWS C
Sbjct: 566 T*NLAAASEKHLVGCFCLRTCRSPPGQYSITRQDRLSVVK*EYRVGRNGWSSICSISLSV 387
Query: 74 XXXXXXXRLAKEVLSITFMA 15
RL + +SITF A
Sbjct: 386 *ALSIFFRLIRYSMSITFSA 327
Score = 100 bits (214), Expect = 4e-021
Identities = 52/127 (40%), Positives = 79/127 (62%)
Frame = -2 / -3
Query: 393 H*TAKR*IEVRQHDVAIFPNEDILWFKITVYNPKHMQVFQCKEHLSYIELCCLL*KIFSR 214
+*T + I+V +HDV IF N+ +L +IT+YNP+HMQ+ QCKE+ S I+ *K SR
Sbjct: 705 N*TPQGQIKVGEHDVPIFTNKYVLRLQITIYNPQHMQILQCKENFSDIKPSSGF*KTSSR 526
Query: 213 LPLAKSVKIPSRAEFHNQA*KLVCLKVSIQGW*KWMVQGLQDLPLGLSSKQLLPTCQRSL 34
L L +++ + H+ A +++C ++ IQG + +V+ LQ LPLGLS + LL
Sbjct: 525 LLLP*DMQVTAWTILHH*ARQIICREIRIQGRKERVVKHLQYLPLGLSPQHLLSAY*VLN 346
Query: 33 IHNLHGK 13
+HNL K
Sbjct: 345 VHNLQCK 325
>gb|AV833557.1|AV833557 AV833557 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags15d19, mRNA sequence
Length = 552
Score = 244 bits (528), Expect = 2e-064
Identities = 100/122 (81%), Positives = 110/122 (90%)
Frame = +1 / +2
Query: 13 FAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCPGGDL 192
FA+KVMD L SRKK+LRAQTEREILQ LDHPFLPTLY++F TD SCLVME+CPGGDL
Sbjct: 185 FALKVMDIEYLISRKKMLRAQTEREILQMLDHPFLPTLYSYFTTDNLSCLVMEYCPGGDL 364
Query: 193 HTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFD 372
H LRQ+QP + FSE AA+FYVAEVLLALEYLHMLG+IYRDLKPEN+LVREDGHIMLSDFD
Sbjct: 365 HVLRQKQPTRCFSEAAARFYVAEVLLALEYLHMLGVIYRDLKPENILVREDGHIMLSDFD 544
Query: 373 LS 378
LS
Sbjct: 545 LS 550
Score = 163 bits (351), Expect = 4e-040
Identities = 76/122 (62%), Positives = 83/122 (68%)
Frame = -3 / -1
Query: 380 RDRSKSDSMMWPSSRTRTFSGLRSRYIIPSICRYSNARSTSAT*NFAACSEKYFPGCLWR 201
RDRSKS+SMM PSSRT FSG RSRYI P+ICRYSNAR TSAT*N AA SEK+ GC
Sbjct: 552 RDRSKSESMMCPSSRTSMFSGFRSRYITPNICRYSNARRTSAT*NLAAASEKHLVGCFCL 373
Query: 200 RV*RSPPGQNSITKHENLSVSK*VYRVGRNGWSRDCXXXXXXXXXXXXXRLAKEVLSITF 21
R RSPPGQ SIT+ + LSV K* YRVGRNGWS C RL + +SITF
Sbjct: 372 RTCRSPPGQYSITRQDRLSVVK*EYRVGRNGWSSICSISLSV*ALSIFFRLIRYSMSITF 193
Query: 20 MA 15
A
Sbjct: 192 SA 187
Score = 100 bits (213), Expect = 5e-021
Identities = 50/120 (41%), Positives = 75/120 (62%)
Frame = -2 / -3
Query: 372 IEVRQHDVAIFPNEDILWFKITVYNPKHMQVFQCKEHLSYIELCCLL*KIFSRLPLAKSV 193
I+V +HDV IF N+ +L +IT+YNP+HMQ+ QCKE+ S I+ *K SRL L +
Sbjct: 544 IKVGEHDVPIFTNKYVLRLQITIYNPQHMQILQCKENFSDIKPSSGF*KTSSRLLLP*DM 365
Query: 192 KIPSRAEFHNQA*KLVCLKVSIQGW*KWMVQGLQDLPLGLSSKQLLPTCQRSLIHNLHGK 13
++ + H+ A +++C ++ IQG + +V+ LQ LPLGLS + LL +HNL K
Sbjct: 364 QVTAWTILHH*ARQIICREIRIQGRKERVVKHLQYLPLGLSPQHLLSAY*VLNVHNLQCK 185
>gb|BU987426.1|BU987426 HF14L16r HF Hordeum vulgare subsp. vulgare cDNA clone HF14L16
5-PRIME, mRNA sequence
Length = 539
Score = 244 bits (528), Expect = 2e-064
Identities = 100/122 (81%), Positives = 110/122 (90%)
Frame = +1 / +1
Query: 13 FAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCPGGDL 192
FA+KVMD L SRKK+LRAQTEREILQ LDHPFLPTLY++F TD SCLVME+CPGGDL
Sbjct: 172 FALKVMDIEYLISRKKMLRAQTEREILQMLDHPFLPTLYSYFTTDNLSCLVMEYCPGGDL 351
Query: 193 HTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFD 372
H LRQ+QP + FSE AA+FYVAEVLLALEYLHMLG+IYRDLKPEN+LVREDGHIMLSDFD
Sbjct: 352 HVLRQKQPTRCFSEAAARFYVAEVLLALEYLHMLGVIYRDLKPENILVREDGHIMLSDFD 531
Query: 373 LS 378
LS
Sbjct: 532 LS 537
Score = 163 bits (351), Expect = 4e-040
Identities = 76/122 (62%), Positives = 83/122 (68%)
Frame = -3 / -1
Query: 380 RDRSKSDSMMWPSSRTRTFSGLRSRYIIPSICRYSNARSTSAT*NFAACSEKYFPGCLWR 201
RDRSKS+SMM PSSRT FSG RSRYI P+ICRYSNAR TSAT*N AA SEK+ GC
Sbjct: 539 RDRSKSESMMCPSSRTSMFSGFRSRYITPNICRYSNARRTSAT*NLAAASEKHLVGCFCL 360
Query: 200 RV*RSPPGQNSITKHENLSVSK*VYRVGRNGWSRDCXXXXXXXXXXXXXRLAKEVLSITF 21
R RSPPGQ SIT+ + LSV K* YRVGRNGWS C RL + +SITF
Sbjct: 359 RTCRSPPGQYSITRQDRLSVVK*EYRVGRNGWSSICSISLSV*ALSIFFRLIRYSMSITF 180
Query: 20 MA 15
A
Sbjct: 179 SA 174
Score = 100 bits (213), Expect = 5e-021
Identities = 50/120 (41%), Positives = 75/120 (62%)
Frame = -2 / -3
Query: 372 IEVRQHDVAIFPNEDILWFKITVYNPKHMQVFQCKEHLSYIELCCLL*KIFSRLPLAKSV 193
I+V +HDV IF N+ +L +IT+YNP+HMQ+ QCKE+ S I+ *K SRL L +
Sbjct: 531 IKVGEHDVPIFTNKYVLRLQITIYNPQHMQILQCKENFSDIKPSSGF*KTSSRLLLP*DM 352
Query: 192 KIPSRAEFHNQA*KLVCLKVSIQGW*KWMVQGLQDLPLGLSSKQLLPTCQRSLIHNLHGK 13
++ + H+ A +++C ++ IQG + +V+ LQ LPLGLS + LL +HNL K
Sbjct: 351 QVTAWTILHH*ARQIICREIRIQGRKERVVKHLQYLPLGLSPQHLLSAY*VLNVHNLQCK 172
>gb|BU983633.1|BU983633 HA30G17r HA Hordeum vulgare subsp. vulgare cDNA clone HA30G17
5-PRIME, mRNA sequence
Length = 493
Score = 216 bits (467), Expect(2) = 4e-064
Identities = 84/99 (84%), Positives = 96/99 (96%)
Frame = +1 / +1
Query: 121 TLYTHFETDKFSCLVMEFCPGGDLHTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGI 300
TLYTHFETD+FSCLVMEFCPGGDLHTLRQ+QPGKYFSE AA+FY AEVLLA+EYLHMLG+
Sbjct: 91 TLYTHFETDRFSCLVMEFCPGGDLHTLRQKQPGKYFSEYAARFYAAEVLLAIEYLHMLGV 270
Query: 301 IYRDLKPENVLVREDGHIMLSDFDLSLRCSVSLTVIKSA 417
+YRDLKPENVLVR+DGHIMLSDFDLSLRC+VS T+I+++
Sbjct: 271 VYRDLKPENVLVRDDGHIMLSDFDLSLRCAVSPTLIRTS 387
Score = 152 bits (327), Expect(2) = 5e-039
Identities = 69/97 (71%), Positives = 76/97 (78%)
Frame = -3 / -3
Query: 410 LITVRLTEQRRDRSKSDSMMWPSSRTRTFSGLRSRYIIPSICRYSNARSTSAT*NFAACS 231
LI V T R+RSKS+S+MWPSSRTRTFSG RS PS+CRYS AR TSA *N AA S
Sbjct: 380 LIRVGETAHLRERSKSESIMWPSSRTRTFSGFRSL*TTPSMCRYSIARRTSAA*NLAAYS 201
Query: 230 EKYFPGCLWRRV*RSPPGQNSITKHENLSVSK*VYRV 120
EKYFPGC RRV +SPPGQNS+T+HENLSVSK V RV
Sbjct: 200 EKYFPGCFCRRVCKSPPGQNSMTRHENLSVSKCV*RV 90
Score = 46.9 bits (96), Expect(2) = 4e-064
Identities = 23/33 (69%), Positives = 25/33 (75%)
Frame = +1 / +3
Query: 34 KTSLASRKKLLRAQTEREILQSLDHPFLPTLYT 132
+ SLASRKKL RAQTEREILQ LD +LYT
Sbjct: 6 RASLASRKKLNRAQTEREILQLLDIHSYNSLYT 104
Score = 27.2 bits (53), Expect(2) = 5e-039
Identities = 13/21 (61%), Positives = 14/21 (66%)
Frame = -2 / -3
Query: 105 VQGLQDLPLGLSSKQLLPTCQ 43
+Q L DLPL LS QLLP Q
Sbjct: 77 IQKL*DLPLSLSPIQLLPAGQ 15
>gb|BJ472879.1|BJ472879 BJ472879 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal39h24 5', mRNA sequence
Length = 452
Score = 188 bits (405), Expect = 2e-047
Identities = 78/132 (59%), Positives = 96/132 (72%)
Frame = +1 / +3
Query: 13 FAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCPGGDL 192
FAMK MDK+ + +R K+ RA EREI LDHPFLPTLYT F+T CL+ +FCPGG+L
Sbjct: 57 FAMKAMDKSVMLNRNKVHRAIIEREIYSLLDHPFLPTLYTSFQTPTHVCLITDFCPGGEL 236
Query: 193 HTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFD 372
+QP K F E +A+FY AEV++ LEYLH LGIIYRDLKPEN+L++ DGHI+L+DFD
Sbjct: 237 FAALDKQPLKIFREDSARFYAAEVVIGLEYLHCLGIIYRDLKPENILLQADGHIVLTDFD 416
Query: 373 LSLRCSVSLTVI 408
LS S VI
Sbjct: 417 LSFLTSSKPHVI 452
>gb|BJ465518.1|BJ465518 BJ465518 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29j23 5', mRNA sequence
Length = 669
Score = 184 bits (397), Expect = 2e-046
Identities = 75/126 (59%), Positives = 90/126 (71%)
Frame = +1 / +2
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
T YFAMK MDK + +R K+ RA ER+IL LDHPFLPTLY F+T CL+ ++ P
Sbjct: 275 TGEYFAMKAMDKNVMLNRNKVHRATAERQILDMLDHPFLPTLYASFQTKTHICLITDYYP 454
Query: 181 GGDLHTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIML 360
GG+L L RQP K E A +FY AEV++ALEYLH GIIYRDLKPEN+L+ DGHI L
Sbjct: 455 GGELFLLLDRQPQKVLREDAVRFYAAEVVIALEYLHCQGIIYRDLKPENILLHRDGHISL 634
Query: 361 SDFDLS 378
+DFDLS
Sbjct: 635 TDFDLS 652
>gb|BQ467802.1|BQ467802 HS04P07r HS Hordeum vulgare subsp. vulgare cDNA clone HS04P07
5-PRIME, mRNA sequence
Length = 436
Score = 167 bits (360), Expect = 3e-041
Identities = 64/109 (58%), Positives = 84/109 (77%)
Frame = +1 / +3
Query: 79 EREILQSLDHPFLPTLYTHFETDKFSCLVMEFCPGGDLHTLRQRQPGKYFSEQAAKFYVA 258
E +L SLDHPF+PTLY + +++C +M++C GGDLH++ +R+PG AA+FY A
Sbjct: 66 ESRVLSSLDHPFVPTLYARLDAGRYACFLMDYCSGGDLHSVLRRRPGGRLPVAAARFYAA 245
Query: 259 EVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFDLSLRCSVSLTV 405
EVLLALEYLH+LG +YRDLKPENVL+R DGH++LSDFDL+L SV V
Sbjct: 246 EVLLALEYLHVLGFVYRDLKPENVLLRGDGHVVLSDFDLALPASVEPAV 392
>gb|CA004277.1|CA004277 HS17B22r HS Hordeum vulgare subsp. vulgare cDNA clone HS17B22
5-PRIME, mRNA sequence
Length = 376
Score = 164 bits (353), Expect = 2e-040
Identities = 61/101 (60%), Positives = 81/101 (80%)
Frame = +1 / +3
Query: 79 EREILQSLDHPFLPTLYTHFETDKFSCLVMEFCPGGDLHTLRQRQPGKYFSEQAAKFYVA 258
E +L SLDHPF+PTLY + +++C +M++C GGDLH++ +R+PG AA+FY A
Sbjct: 66 ESRVLSSLDHPFVPTLYARLDAGRYACFLMDYCSGGDLHSVLRRRPGGRLPVAAARFYAA 245
Query: 259 EVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFDLSL 381
EVLLALEYLH+LG +YRDLKPENVL+R DGH++LSDFDL+L
Sbjct: 246 EVLLALEYLHVLGFVYRDLKPENVLLRGDGHVVLSDFDLAL 368
>gb|BU973765.1|BU973765 HB25O11r BC Hordeum vulgare subsp. vulgare cDNA clone HB25O11
5-PRIME, mRNA sequence
Length = 500
Score = 159 bits (341), Expect = 1e-038
Identities = 63/70 (90%), Positives = 66/70 (94%)
Frame = +1 / +2
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
T+ YFAMK+MDK SLA RKKLLRAQTEREILQ LDHPFLPTLYTHFETDKFSCLVMEFCP
Sbjct: 290 TKCYFAMKIMDKASLAGRKKLLRAQTEREILQCLDHPFLPTLYTHFETDKFSCLVMEFCP 469
Query: 181 GGDLHTLRQR 210
GGDLHTLRQ+
Sbjct: 470 GGDLHTLRQK 499
>gb|CA021581.1|CA021581 HZ40I04r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ40I04
5-PRIME, mRNA sequence
Length = 637
Score = 132 bits (282), Expect = 1e-030
Identities = 55/58 (94%), Positives = 57/58 (98%)
Frame = +1 / +2
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEF 174
T+S+FAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTH ETDKFSCLVMEF
Sbjct: 464 TKSHFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHXETDKFSCLVMEF 637
>gb|BQ472656.1|BQ472656 HB01O12r BC Hordeum vulgare subsp. vulgare cDNA clone HB01O12
5-PRIME, mRNA sequence
Length = 669
Score = 65.7 bits (137), Expect(2) = 2e-024
Identities = 26/71 (36%), Positives = 44/71 (61%)
Frame = +1 / +3
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
+RS +AMK + K+ + R ++ + ER +L +D ++ LY F+ D+F L+ME+ P
Sbjct: 54 SRSVYAMKKLKKSEMLRRGQVEHVKAERNLLAEVDSAYIVKLYYSFQDDEFLYLIMEYLP 233
Query: 181 GGDLHTLRQRQ 213
GGD+ TL R+
Sbjct: 234 GGDMMTLLMRK 266
Score = 65.2 bits (136), Expect(2) = 2e-024
Identities = 25/49 (51%), Positives = 36/49 (73%)
Frame = +1 / +3
Query: 229 SEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFDL 375
+E AKFY+AE +LA+E +H I+RD+KP+N+L+ GH+ LSDF L
Sbjct: 276 TEDEAKFYIAETVLAIESIHKHNYIHRDIKPDNLLLDLSGHLKLSDFGL 422
>gb|CA014505.1|CA014505 HT11I16r HT Hordeum vulgare subsp. vulgare cDNA clone HT11I16
5-PRIME, mRNA sequence
Length = 670
Score = 62.5 bits (130), Expect(2) = 1e-022
Identities = 24/49 (48%), Positives = 35/49 (71%)
Frame = +1 / +1
Query: 229 SEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFDL 375
+E A+FYV E +LA+E +H I+RD+KP+N+L+ GH+ LSDF L
Sbjct: 385 TEDEARFYVGETVLAIEAIHRHNYIHRDIKPDNLLLDRYGHLRLSDFGL 531
Score = 62.5 bits (130), Expect(2) = 1e-022
Identities = 25/71 (35%), Positives = 43/71 (60%)
Frame = +1 / +1
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
T + +AMK + K+ + R ++ + ER +L +DH + LY F+ ++F L+ME+ P
Sbjct: 163 TGNVYAMKKLKKSEMLRRGQVEHVRAERNLLAEVDHHCIVKLYCSFQDNEFLYLIMEYLP 342
Query: 181 GGDLHTLRQRQ 213
GGD+ TL R+
Sbjct: 343 GGDMMTLLMRK 375
>gb|BU988941.1|BU988941 HF19D14r HF Hordeum vulgare subsp. vulgare cDNA clone HF19D14
5-PRIME, mRNA sequence
Length = 516
Score = 65.7 bits (137), Expect(2) = 2e-022
Identities = 26/71 (36%), Positives = 44/71 (61%)
Frame = +1 / +3
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
+RS +AMK + K+ + R ++ + ER +L +D ++ LY F+ D+F L+ME+ P
Sbjct: 159 SRSVYAMKKLKKSEMLRRGQVEHVKAERNLLAEVDSAYIVKLYYSFQDDEFLYLIMEYLP 338
Query: 181 GGDLHTLRQRQ 213
GGD+ TL R+
Sbjct: 339 GGDMMTLLMRK 371
Score = 58.3 bits (121), Expect(2) = 2e-022
Identities = 22/45 (48%), Positives = 33/45 (73%)
Frame = +1 / +3
Query: 229 SEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLS 363
+E AKFY+AE +LA+E +H I+RD+KP+N+L+ GH+ LS
Sbjct: 381 TEDEAKFYIAETVLAIESIHKHNYIHRDIKPDNLLLDLSGHLKLS 515
>gb|BG365974.2|BG365974 HVSMEi0005I24f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0005I24f, mRNA sequence
Length = 936
Score = 70.3 bits (147), Expect(2) = 3e-021
Identities = 26/51 (50%), Positives = 39/51 (76%)
Frame = +1 / +1
Query: 226 FSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFDLS 378
F E+ A+ Y AE++ A+ +LH GI++RDLKPEN+L+ +GH ML+DF L+
Sbjct: 262 FREELARIYTAEIISAVAHLHANGIMHRDLKPENILLDAEGHAMLTDFGLA 414
Score = 50.1 bits (103), Expect(2) = 3e-021
Identities = 24/64 (37%), Positives = 34/64 (53%)
Frame = +1 / +1
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
T +AMKVM K + + + ER+IL +DHPF+ L F+T LV++F
Sbjct: 43 TSEIYAMKVMRKDKILEKNHSEYMKAERDILTKVDHPFVVQLRYSFQTKYRLYLVLDFVN 222
Query: 181 GGDL 192
GG L
Sbjct: 223 GGHL 234
>gb|BG367473.2|BG367473 HVSMEi0012G07f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0012G07f, mRNA sequence
Length = 704
Score = 68.9 bits (144), Expect(2) = 8e-021
Identities = 25/51 (49%), Positives = 39/51 (76%)
Frame = +1 / +2
Query: 226 FSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFDLS 378
F E+ A+ Y AE++ A+ +LH GI++RDLKPEN+L+ +GH +L+DF L+
Sbjct: 455 FREELARIYTAEIISAVAHLHANGIMHRDLKPENILLDAEGHALLTDFGLA 607
Score = 50.1 bits (103), Expect(2) = 8e-021
Identities = 24/64 (37%), Positives = 34/64 (53%)
Frame = +1 / +2
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
T +AMKVM K + + + ER+IL +DHPF+ L F+T LV++F
Sbjct: 236 TSEIYAMKVMRKDKILEKNHSEYMKAERDILTKVDHPFVVQLRYSFQTKYRLYLVLDFVN 415
Query: 181 GGDL 192
GG L
Sbjct: 416 GGHL 427
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 238,389,061
Number of Sequences: 312970
Number of extensions: 3971681
Number of successful extensions: 107766
Number of sequences better than 1.0e-020: 20
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 4315697579
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)