TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH10g11.yg.2.1
(632 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 297 6e-087
gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA li... 176 1e-043
gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA li... 141 3e-033
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 297 bits (642), Expect(2) = 6e-087
Identities = 125/150 (83%), Positives = 138/150 (92%)
Frame = -3 / +3
Query: 453 REVEIYRVKLPGELKIGEGKPENQNHALIFTRGDAVQTIDMNQDNYFEEALKMRNLLEEF 274
++ EIY VKLPG K+GEGKPENQNHA+IFTRG+AVQTIDMNQDNYFEEALKMRNLLEEF
Sbjct: 3906 KDKEIYSVKLPGNPKLGEGKPENQNHAVIFTRGNAVQTIDMNQDNYFEEALKMRNLLEEF 4085
Query: 273 NRYYGIRKPKILGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLADPLKVRMHYGHPDV 94
++ +G KP ILGVREHVFTGSVSSLA FMS QETSFVTLGQRVL++PLKVRMHYGHPDV
Sbjct: 4086 SQNHGKFKPSILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLSNPLKVRMHYGHPDV 4265
Query: 93 FDRLWFLGRGGISKASRIINISEDIFAGFN 4
FDR++ + RGGISKASRIINISEDIFAGFN
Sbjct: 4266 FDRIFHITRGGISKASRIINISEDIFAGFN 4355
Score = 120 bits (256), Expect = 9e-027
Identities = 59/147 (40%), Positives = 85/147 (57%)
Frame = +1 / -2
Query: 4 VETSKNVLTNVDYS*CFTDTTSAQKPKTIKNIWVAIMHSDFKWISQNTLPQSDKTCLLGR 183
++ K +LT++D + TD T +IK++WV IMHS F+WI +NTL KT L
Sbjct: 4355 IKPCKYILTDIDDTGRLTDATPRNMKYSIKHVWVTIMHSHFQWIRKNTLS*CHKTSFLI* 4176
Query: 184 HEPSKRRN*TREDVFPNPKNFRLANSIITIELF**VSHL*SFFKVVVLVHINSLNCIAPC 363
H+ SK N + ED+F N KN RL ++I E +SH ++VVL+HIN L I+
Sbjct: 4175 HK*SKGGNTSGEDMFSNTKN*RLELAMILREFLKQISHFECLLEIVVLIHINGLYSISAS 3996
Query: 364 EDECMILIFWLSFANLQLSRQLNPIDF 444
E M+LIF + L ++RQ N I+F
Sbjct: 3995 EYYSMVLIFGFTLPKLWIARQFNRINF 3915
Score = 103 bits (219), Expect = 1e-021
Identities = 50/143 (34%), Positives = 75/143 (52%)
Frame = -2 / +2
Query: 442 NLSG*VAWRVEDWRRKARKSESCTHLHKGRCSSNY*YEPRQLL*RSSKDEKPTRRVQSLL 263
NL *+AW+ + W RKS+ C ++H +C +N+*YE QL +++ K + S
Sbjct: 3917 NLFC*IAWQSKAWGG*TRKSKPCCNIHSRKCCTNH*YESGQLFRGGTQNAKSA*GILSKS 4096
Query: 262 WNSQA*NSWGSGTRLHGFSFFSCLVHVCPGDKFCHXXXXXXXXXXXSQNALWPPRCF*SS 83
W QA NSW T LH F C ++V G+ FC S+NALW PR * +
Sbjct: 4097 WQVQAFNSWC*RTCLHRKCFLPCFIYVKSGN*FCDIRTACSF*STESENALWSPRRV**N 4276
Query: 82 LVFGPRWYQ*SIKNNQH**GHFC 14
+ + W+Q* + +Q+ *G+ C
Sbjct: 4277 ISYYEGWHQ*GVPYHQYQ*GYIC 4345
Score = 83.5 bits (176), Expect(3) = 1e-027
Identities = 37/49 (75%), Positives = 39/49 (79%)
Frame = +2 / -3
Query: 89 SKTSGWP*CILTLSGSARTRCPRVTKLVSWADMNQAREETEPVKTCSRT 235
S TSG P*CILT SG RTRCP VTKLVS D+N+AREET PVKTCS T
Sbjct: 4270 SNTSG*P*CILTFSGLERTRCPNVTKLVS*FDINEAREETLPVKTCSLT 4124
Score = 44.1 bits (90), Expect(2) = 6e-087
Identities = 17/34 (50%), Positives = 22/34 (64%)
Frame = -3 / +3
Query: 624 CQVYXQQKAKNDPNAXXILELMKNYEALRVAYVD 523
CQ+Y QK + P A I LM+ EALR+AY+D
Sbjct: 3729 CQIYGVQKGEGKPEAADIALLMQRNEALRIAYID 3830
Score = 41.4 bits (84), Expect(3) = 1e-027
Identities = 22/32 (68%), Positives = 24/32 (75%)
Frame = +2 / -3
Query: 275 NSSSRFLIFRASSK*LSWFISIV*TASPLVKM 370
NSSSRF I ASSK*LS FIS+V TA P V +
Sbjct: 4084 NSSSRFRILSASSK*LS*FISMVCTAFPRVNI 3989
Score = 35.4 bits (71), Expect(3) = 1e-027
Identities = 14/22 (63%), Positives = 19/22 (86%)
Frame = +2 / -3
Query: 2 QLKPAKMSSLMLIILDALLIPP 67
+L PA +SSL+L+I DALL+PP
Sbjct: 4357 ELNPANISSLILMIRDALLMPP 4292
>gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3g22 5', mRNA sequence
Length = 611
Score = 176 bits (379), Expect = 1e-043
Identities = 70/86 (81%), Positives = 80/86 (93%)
Frame = -3 / +2
Query: 261 GIRKPKILGVREHVFTGSVSSLAWFMSAQETSFVTLGQRVLADPLKVRMHYGHPDVFDRL 82
G+R P ILGVREH+FTGSVSSLAWFMS QETSFVT+GQRVLA+PL+VR HYGHPD+FDRL
Sbjct: 8 GVRYPTILGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLANPLRVRFHYGHPDIFDRL 187
Query: 81 WFLGRGGISKASRIINISEDIFAGFN 4
+ L RGG+SKAS+IIN+SEDIFAGFN
Sbjct: 188 FHLTRGGVSKASKIINLSEDIFAGFN 265
Score = 106 bits (226), Expect = 1e-022
Identities = 51/78 (65%), Positives = 54/78 (69%)
Frame = +2 / -3
Query: 2 QLKPAKMSSLMLIILDALLIPPRPKNQRRSKTSGWP*CILTLSGSARTRCPRVTKLVSWA 181
+L PA MSSL LIILDALL PP + + RSK SG P* TL G A TRCP VTKLVSW
Sbjct: 267 ELNPANMSSLRLIILDALLTPPLVRWKSRSKISGCP**NRTLKGLASTRCPIVTKLVSWF 88
Query: 182 DMNQAREETEPVKTCSRT 235
DMN A EET PV CS T
Sbjct: 87 DMNHASEETLPVNICSLT 34
>gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags28g06 5', mRNA sequence
Length = 666
Score = 141 bits (303), Expect = 3e-033
Identities = 56/59 (94%), Positives = 59/59 (100%)
Frame = -3 / +3
Query: 177 QETSFVTLGQRVLADPLKVRMHYGHPDVFDRLWFLGRGGISKASRIINISEDIFAGFNC 1
+ETSFVTLGQRVLA+PLKVRMHYGHPDVFDRLWFLGRGGISKASR+INISEDIFAGFNC
Sbjct: 21 RETSFVTLGQRVLANPLKVRMHYGHPDVFDRLWFLGRGGISKASRVINISEDIFAGFNC 197
Score = 124 bits (266), Expect = 4e-028
Identities = 53/58 (91%), Positives = 54/58 (93%)
Frame = +2 / -3
Query: 2 QLKPAKMSSLMLIILDALLIPPRPKNQRRSKTSGWP*CILTLSGSARTRCPRVTKLVS 175
QL PAK+SSLMLI LDALLIPPRPKNQRRS TSGWP*CILTLSG ARTRCPRVTKLVS
Sbjct: 196 QLNPAKISSLMLITLDALLIPPRPKNQRRSNTSGWP*CILTLSGLARTRCPRVTKLVS 23
Score = 114 bits (243), Expect = 5e-025
Identities = 43/57 (75%), Positives = 55/57 (96%)
Frame = +1 / -2
Query: 1 TVETSKNVLTNVDYS*CFTDTTSAQKPKTIKNIWVAIMHSDFKWISQNTLPQSDKTC 171
T+E+SK++LT+VDYS*CFT+TTSAQ+PKTIK+IWVAIMHS+ +W+S N+LPQSDKTC
Sbjct: 197 TIESSKDILTDVDYS*CFTNTTSAQEPKTIKHIWVAIMHSNLEWVS*NSLPQSDKTC 27
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 264,862,602
Number of Sequences: 312970
Number of extensions: 3741313
Number of successful extensions: 31801
Number of sequences better than 1.0e-020: 4
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6744256731
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)