TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCG13d12.yg.2.1
(755 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AY177665.1| Hordeum vulgare subsp. vulgare putative call... 327 5e-091
gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA li... 237 4e-062
gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA li... 148 4e-035
>gb|AY177665.1| Hordeum vulgare subsp. vulgare putative callose synthase mRNA,
complete cds
Length = 6180
Score = 327 bits (708), Expect(2) = 5e-091
Identities = 128/163 (78%), Positives = 149/163 (91%)
Frame = -2 / +3
Query: 502 DQVIYKIKLPGNAILGEGKPENQNHAIIFTRGECLQTIDMNQEHYMEEALKMRNLLQEFE 323
D+ IY +KLPGN LGEGKPENQNHA+IFTRG +QTIDMNQ++Y EEALKMRNLL+EF
Sbjct: 3909 DKEIYSVKLPGNPKLGEGKPENQNHAVIFTRGNAVQTIDMNQDNYFEEALKMRNLLEEFS 4088
Query: 322 KKHGVRYPSMLGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLANPLRVRFHYGHPDIF 143
+ HG PS+LGVREH+FTGSVSSLA FMSNQETSFVT+GQRVL+NPL+VR HYGHPD+F
Sbjct: 4089 QNHGKFKPSILGVREHVFTGSVSSLASFMSNQETSFVTLGQRVLSNPLKVRMHYGHPDVF 4268
Query: 142 DRLFHITRGGVSKASKIINLSEDIFAGFNSTLREGNVTHHEYL 14
DR+FHITRGG+SKAS+IIN+SEDIFAGFNSTLR+GN+THHEY+
Sbjct: 4269 DRIFHITRGGISKASRIINISEDIFAGFNSTLRQGNITHHEYI 4397
Score = 148 bits (317), Expect = 4e-035
Identities = 67/142 (47%), Positives = 103/142 (72%)
Frame = +2 / -2
Query: 17 VLVVSNIAFTQCGIESCKYVLTEIDNFGCFTHTTPRNVEKAIEDIRMTIMKTNPQRIGKH 196
+L+VS + TQ I+ CKY+LT+ID+ G T TPRN++ +I+ + +TIM ++ Q I K+
Sbjct: 4394 ILMVSYVPLTQSRIKPCKYILTDIDDTGRLTDATPRNMKYSIKHVWVTIMHSHFQWIRKN 4215
Query: 197 SLSNCYETSFLI*HEPCKG*HTARKYVFSDSKHRWISHSMFLLKLLQQISHFQSLLHIVL 376
+LS C++TSFLI*H+ KG +T+ + +FS++K+ + +M L + L+QISHF+ LL IV+
Sbjct: 4214 TLS*CHKTSFLI*HK*SKGGNTSGEDMFSNTKN*RLELAMILREFLKQISHFECLLEIVV 4035
Query: 377 LIHIYSLEAFASGEYYCMVLIF 442
LIHI L + ++ EYY MVLIF
Sbjct: 4034 LIHINGLYSISASEYYSMVLIF 3969
Score = 147 bits (316), Expect(2) = 2e-042
Identities = 72/114 (63%), Positives = 80/114 (70%)
Frame = +3 / -3
Query: 18 YSW*VTLPSRNVELNPANMSSLRLIILDALLTPPLVMWKRRSKISG*P**KRTLNGLAST 197
YSW*V P R VELNPAN+SSL L+I DALL PPLV+ S SG*P* T +GL T
Sbjct: 4393 YSW*VMFP*RRVELNPANISSLILMIRDALLMPPLVI*NILSNTSG*P*CILTFSGLERT 4214
Query: 198 RCPIVTKLVS*FDMNHARDDTLPVNMCSLTPSIDGYLTPCFFSNSCSRFLIFKA 359
RCP VTKLVS*FD+N AR++TLPV CSLTP I+G P F NS SRF I A
Sbjct: 4213 RCPNVTKLVS*FDINEAREETLPVKTCSLTPRIEGLNLP*F*ENSSSRFRILSA 4052
Score = 98.2 bits (208), Expect(2) = 6e-027
Identities = 41/96 (42%), Positives = 61/96 (63%)
Frame = -1 / +2
Query: 443 RKSEPCNNIHQRRMPPNYRYESGALYGGGFENEKSAARV*EETWSEISIDAWSQRTHIYG 264
RKS+PC NIH R+ N+ YESG L+ GG +N KSA + ++W + ++W RT ++
Sbjct: 3968 RKSKPCCNIHSRKCCTNH*YESGQLFRGGTQNAKSA*GILSKSWQVQAFNSWC*RTCLHR 4147
Query: 263 QCVIPCMVHVKSGN*FRNNWTTSACQSVEGSFSLWS 156
+C +PC ++VKSGN*F + T + S E +LWS
Sbjct: 4148 KCFLPCFIYVKSGN*FCDIRTACSF*STESENALWS 4255
Score = 45.1 bits (92), Expect(2) = 2e-042
Identities = 17/24 (70%), Positives = 18/24 (75%)
Frame = +3 / -3
Query: 408 PLVNIIAWF*FSGFPSPKMALPGS 479
P VNI AWF*FSG PSP LPG+
Sbjct: 4003 PRVNITAWF*FSGLPSPSFGLPGN 3932
Score = 42.3 bits (86), Expect(2) = 6e-027
Identities = 17/35 (48%), Positives = 25/35 (71%)
Frame = -1 / +2
Query: 128 HYEGWCE*SIQNYQSQ*GHICRIQFHIA*RQCYSP 24
+YEGW +* + +Q Q*G+ICR+ F+ A R+ SP
Sbjct: 4283 YYEGWHQ*GVPYHQYQ*GYICRV*FYSASREHNSP 4387
Score = 27.6 bits (54), Expect(2) = 5e-091
Identities = 15/46 (32%), Positives = 19/46 (41%)
Frame = -2 / +3
Query: 742 ADMXXXYXXXXXXYGIQXXXXEXXAHDILRLXTXYPSXXXAYIDEV 605
AD+ Y YG+Q + A DI L + AYID V
Sbjct: 3699 ADLKFTYVVTCQIYGVQKGEGKPEAADIALLMQRNEALRIAYIDVV 3836
>gb|AV923029.1|AV923029 AV923029 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3g22 5', mRNA sequence
Length = 611
Score = 237 bits (513), Expect = 4e-062
Identities = 96/100 (96%), Positives = 100/100 (100%)
Frame = -2 / +2
Query: 313 GVRYPSMLGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLANPLRVRFHYGHPDIFDRL 134
GVRYP++LGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLANPLRVRFHYGHPDIFDRL
Sbjct: 8 GVRYPTILGVREHIFTGSVSSLAWFMSNQETSFVTIGQRVLANPLRVRFHYGHPDIFDRL 187
Query: 133 FHITRGGVSKASKIINLSEDIFAGFNSTLREGNVTHHEYL 14
FH+TRGGVSKASKIINLSEDIFAGFNSTLREGNVTHHEY+
Sbjct: 188 FHLTRGGVSKASKIINLSEDIFAGFNSTLREGNVTHHEYM 307
Score = 189 bits (407), Expect = 2e-047
Identities = 84/99 (84%), Positives = 88/99 (88%)
Frame = +3 / -3
Query: 18 YSW*VTLPSRNVELNPANMSSLRLIILDALLTPPLVMWKRRSKISG*P**KRTLNGLAST 197
YS VT PSR+VELNPANMSSLRLIILDALLTPPLV WK RSKISG P** RTL GLAST
Sbjct: 303 YS*CVTFPSRSVELNPANMSSLRLIILDALLTPPLVRWKSRSKISGCP**NRTLKGLAST 124
Query: 198 RCPIVTKLVS*FDMNHARDDTLPVNMCSLTPSIDGYLTP 314
RCPIVTKLVS FDMNHA ++TLPVN+CSLTPSI GYLTP
Sbjct: 123 RCPIVTKLVSWFDMNHASEETLPVNICSLTPSIVGYLTP 7
Score = 139 bits (298), Expect = 2e-032
Identities = 56/100 (56%), Positives = 80/100 (80%)
Frame = +2 / -2
Query: 17 VLVVSNIAFTQCGIESCKYVLTEIDNFGCFTHTTPRNVEKAIEDIRMTIMKTNPQRIGKH 196
V ++ N++FTQC IES KYVLT+IDN CF +TTP +E++I+DIRM+IMK+NP RIGK+
Sbjct: 304 VFMMCNVSFTQC*IESSKYVLTKIDNLRCFAYTTPCEMEESIKDIRMSIMKSNP*RIGKY 125
Query: 197 SLSNCYETSFLI*HEPCKG*HTARKYVFSDSKHRWISHSM 316
+LSN ++T L+*HEP K +TA +Y+FS +K+ WI H++
Sbjct: 124 TLSNSHKTCLLV*HEPRKRRNTASEYMFSYTKYCWIPHTI 5
Score = 126 bits (269), Expect = 2e-028
Identities = 51/96 (53%), Positives = 68/96 (70%)
Frame = -1 / +1
Query: 314 WSEISIDAWSQRTHIYGQCVIPCMVHVKSGN*FRNNWTTSACQSVEGSFSLWSS*YLRSP 135
W E+S + W +RT+I+ QC C+VHVK + F + WT CQS +GS SLW+S*YL S
Sbjct: 7 WCEVSNNTWCKRTYIHWQCFFACVVHVKPRDKFCDYWTACTCQSFKGSISLWTS*YL*ST 186
Query: 134 FPHYEGWCE*SIQNYQSQ*GHICRIQFHIA*RQCYS 27
P ++GWC+ SI +YQS *GHIC IQF+ A*R+ Y+
Sbjct: 187 LPSHKGWCKQSI*DYQS**GHICWIQFNTA*RKRYT 294
Score = 102 bits (217), Expect = 3e-021
Identities = 57/101 (56%), Positives = 64/101 (63%)
Frame = -3 / +3
Query: 315 ME*DIHRCLESENTYLRAVCHPLHGSCQIRKLVS*QLDNECLPIR*GFVFIMVILISSIA 136
M * I + L EN Y AV L GSCQ ++ V * LD+ LPI *GF FIM ILIS I
Sbjct: 6 MV*GIQQYLV*ENIYSLAVFLRLRGSCQTKRQVL*LLDSVYLPIL*GFDFIMDILISLID 185
Query: 135 FSTLRGVV*VKHPKLSISVRTYLQDSIPHCVKAMLLTTSTC 13
S +GVV* KH +LSI VRTYL DSI HCVK L +TC
Sbjct: 186 SSISQGVV*AKHLRLSILVRTYLLDSIQHCVKETLHIMNTC 308
Score = 101 bits (216), Expect = 4e-021
Identities = 53/92 (57%), Positives = 58/92 (63%)
Frame = +1 / -1
Query: 40 LHAMWN*ILQICPH*D**FWMLYSHHPS*CGKGDRRYQDDHNENEPSTDWQALVVQLLRN 219
LHA+ N*I QICPH*D** ML HHP * G+ D+RYQD HNE EP DWQ VQ +N
Sbjct: 281 LHAVLN*IQQICPH*D**S*MLCLHHPL*DGRVDQRYQDVHNEIEPLKDWQVHAVQ*SQN 102
Query: 220 *FPDLT*TMQGMTHCP*ICVL*LQASMDISLH 315
LT*T Q HC *I VL Q +D S H
Sbjct: 101 LSLGLT*TTQAKKHCQ*IYVLLHQVLLDTSHH 6
>gb|BJ462458.1|BJ462458 BJ462458 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags28g06 5', mRNA sequence
Length = 666
Score = 148 bits (317), Expect = 4e-035
Identities = 56/72 (77%), Positives = 67/72 (93%)
Frame = -2 / +3
Query: 229 QETSFVTIGQRVLANPLRVRFHYGHPDIFDRLFHITRGGVSKASKIINLSEDIFAGFNST 50
+ETSFVT+GQRVLANPL+VR HYGHPD+FDRL+ + RGG+SKAS++IN+SEDIFAGFN T
Sbjct: 21 RETSFVTLGQRVLANPLKVRMHYGHPDVFDRLWFLGRGGISKASRVINISEDIFAGFNCT 200
Query: 49 LREGNVTHHEYL 14
LR GNVTHHEY+
Sbjct: 201 LRGGNVTHHEYI 236
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 304,843,400
Number of Sequences: 312970
Number of extensions: 4363058
Number of successful extensions: 22963
Number of sequences better than 1.0e-020: 4
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8483341800
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)