TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCF7a10.yg.2.1
(523 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU973855.1|BU973855 HB26C19r BC Hordeum vulgare subsp. v... 238 8e-076
gb|CA009582.1|CA009582 HU14I15r HU Hordeum vulgare subsp. v... 235 2e-063
gb|BG300460.1|BG300460 HVSMEb0017C05f Hordeum vulgare seedl... 187 2e-062
gb|BF255654.2|BF255654 HVSMEf0007H02f Hordeum vulgare seedl... 156 2e-055
gb|BJ454539.1|BJ454539 BJ454539 K. Sato unpublished cDNA li... 165 5e-054
gb|BJ468853.1|BJ468853 BJ468853 K. Sato unpublished cDNA li... 156 1e-053
gb|AV922700.1|AV922700 AV922700 K. Sato unpublished cDNA li... 156 2e-052
gb|BQ472666.1|BQ472666 HB01N17r BC Hordeum vulgare subsp. v... 156 6e-052
gb|CA027499.1|CA027499 HZ59C02r HZ Hordeum vulgare subsp. v... 152 7e-051
gb|BJ462697.1|BJ462697 BJ462697 K. Sato unpublished cDNA li... 156 5e-049
gb|BU999595.1|BU999595 HI14P19r HI Hordeum vulgare subsp. v... 156 9e-048
gb|BM374688.1|BM374688 EBma05_SQ002_H10_R maternal, 12 DPA,... 153 7e-041
gb|BI947860.1|BI947860 HVSMEl0007D23f Hordeum vulgare spike... 132 4e-039
gb|BE195440.3|BE195440 HVSMEh0089K10f Hordeum vulgare 5-45 ... 116 1e-038
gb|BJ463647.1|BJ463647 BJ463647 K. Sato unpublished cDNA li... 108 5e-037
gb|BI952630.1|BI952630 HVSMEm0007D23f Hordeum vulgare green... 84 1e-026
gb|CA027598.1|CA027598 HZ59G21r HZ Hordeum vulgare subsp. v... 100 2e-025
gb|BU974311.1|BU974311 HB27I16r BC Hordeum vulgare subsp. v... 65 1e-022
>gb|BU973855.1|BU973855 HB26C19r BC Hordeum vulgare subsp. vulgare cDNA clone HB26C19
5-PRIME, mRNA sequence
Length = 554
Score = 238 bits (514), Expect(2) = 8e-076
Identities = 97/114 (85%), Positives = 106/114 (92%)
Frame = +3 / +2
Query: 3 FDARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGS 182
FDARRKP N+GK+I AL+ LCIF LKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGS
Sbjct: 23 FDARRKPHNIGKVIVALLFLALCIFVLKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGS 202
Query: 183 HAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
HA+LRLL+D+YRVTIVDNLSRGN GAVKVLQ LFP+PGRLQFI+ADLGDQKSV+
Sbjct: 203 HASLRLLKDNYRVTIVDNLSRGNKGAVKVLQELFPEPGRLQFIYADLGDQKSVN 364
Score = 209 bits (450), Expect(2) = 4e-065
Identities = 89/113 (78%), Positives = 93/113 (82%)
Frame = -1 / -3
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
TDF SPRSA MN SLPG GN RTFTAP+FPL+RLSTMVTR*LS S ACEPI*PAP
Sbjct: 360 TDF*SPRSA*MN*SLPGSGNNSWRTFTAPLFPLERLSTMVTR*LSFNSRNEACEPI*PAP 181
Query: 160 PVTKTWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFPTLRGFRLASK 2
PVTKTWVTPGSWRENTLLPPKPGDCFR NIQS ++RA M FP L G RLASK
Sbjct: 180 PVTKTWVTPGSWRENTLLPPKPGDCFRTNIQSAKKSRATMTFPILCGLRLASK 22
Score = 182 bits (393), Expect(2) = 7e-046
Identities = 71/103 (68%), Positives = 81/103 (78%)
Frame = +2 / +1
Query: 32 WKNHCSPGPHNTLYICSEAISWFWWQ*CVFSP*TWCYPRLSNRRSWLYWFTCRFKALTG* 211
W++HC P +TLYICSEAISWFWWQ*CVFSP*TW YP LS+RRSWLYWFTC A+ *
Sbjct: 52 WESHCGPALLSTLYICSEAISWFWWQ*CVFSP*TWGYPCLSDRRSWLYWFTCLITAIKR* 231
Query: 212 LSSYHCG*SI*RKYGSSKGSAGLISAAW*TTIHLC*SGGPEIC 340
LSSYHCG S *RK SSKGS ++S AW T++HLC S G +IC
Sbjct: 232 LSSYHCGQSF*RK*RSSKGSPRIVSGAWETSVHLCRSWGSKIC 360
Score = 182 bits (392), Expect(2) = 2e-056
Identities = 82/113 (72%), Positives = 92/113 (81%)
Frame = -3 / -2
Query: 341 YRFLVPQISKDEL*STRLRK*ALQNLYCSHISSR*IIHNGNSIAIL*EP*SGM*TNITSS 162
+RFL+P+I DEL S RLRK L+NLYCS ISSR I+HNGNSI I * P* GM*TNI SS
Sbjct: 361 HRFLIPKICIDELKSPRLRKQFLENLYCSFISSRKIVHNGNSIVIF**P**GM*TNIASS 182
Query: 161 SCY*DVGNTRFMARKHTIATKTRRLLQSKYTECCEDQGCNDFSNITRLPPCIK 3
SC+*D+GN RFMARKHTIATKTRRLLQ+KYTEC E+QG NDF NI R P I+
Sbjct: 181 SCH*DMGNPRFMARKHTIATKTRRLLQNKYTEC*EEQGHNDFPNIVRFTPSIE 23
Score = 142 bits (304), Expect(2) = 7e-044
Identities = 70/113 (61%), Positives = 75/113 (66%)
Frame = -2 / -1
Query: 339 QISGPPDQQR*IVVYQAAEISPAEPLLLPYFL*IDYPQW*LDSYPVRALKRHVNQYNQXX 160
QI P D R* V QA E EPLLL YFL* D PQW*LDSY + A+ RHVNQY+Q
Sbjct: 359 QIFDPQDLHR*TEVSQAPETILGEPLLLLYFL*KDCPQW*LDSYLLIAVMRHVNQYSQLL 180
Query: 159 XXXXRG*HQVHGEKTHYCHQNQEIASEQIYRVL*GPGLQ*FFQHYEASALHQS 1
G* QVHGEKTHYCHQNQEIASEQIYRVL G Q* Q+ A H+S
Sbjct: 179 LSLRHG*PQVHGEKTHYCHQNQEIASEQIYRVLRRAGPQ*LSQYCAVYA*HRS 21
Score = 107 bits (229), Expect = 4e-023
Identities = 51/75 (68%), Positives = 58/75 (77%)
Frame = +1 / +3
Query: 115 CFLAMNLVLPTS**QEELVILVHMPL*GSYRIAIELPLWIIYLEEIWEQ*RFCRAYFRSL 294
CFLAMNL LP S**QEEL ILVHMP G +I IELPLW I+LEEI EQ*RF + FRSL
Sbjct: 135 CFLAMNLGLPMS**QEELAILVHMPHYGY*KITIELPLWTIFLEEIKEQ*RFSKNCFRSL 314
Query: 295 VDYNSSLLIWGTRNL 339
D++SS+ I G +NL
Sbjct: 315 GDFSSSMQILGIKNL 359
Score = 65.2 bits (136), Expect(2) = 8e-076
Identities = 26/29 (89%), Positives = 28/29 (96%)
Frame = +1 / +2
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VNKIF+ENAFDAVMHFAA AYVGEST+EP
Sbjct: 359 VNKIFSENAFDAVMHFAAVAYVGESTMEP 445
Score = 58.8 bits (122), Expect(2) = 4e-065
Identities = 21/28 (75%), Positives = 23/28 (82%)
Frame = -2 / -2
Query: 522 GFQCALSHISXSCKVHHSIKCIFSKYLV 439
GF C L+HI SC+VHHSIKCIF KYLV
Sbjct: 445 GFHCTLTHIGNSCEVHHSIKCIFRKYLV 362
Score = 56.1 bits (116), Expect(2) = 2e-056
Identities = 21/28 (75%), Positives = 25/28 (89%)
Frame = -1 / -1
Query: 523 GVPMCSLPHKXQLQSASQHQMHFQQISC 440
GVP+ S PH+ QL+SASQHQMHFQ+ISC
Sbjct: 446 GVPLYSHPHRQQLRSASQHQMHFQKISC 363
Score = 54.7 bits (113), Expect(2) = 7e-044
Identities = 25/29 (86%), Positives = 25/29 (86%)
Frame = -3 / -3
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
GS VLSPT*A AAKCITASNAFS NIL T
Sbjct: 444 GSIVLSPT*ATAAKCITASNAFSENILFT 358
Score = 21.7 bits (41), Expect(2) = 7e-046
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +3 / +1
Query: 474 DALCSXXLCGREHIGT 521
DAL S LCG E+ GT
Sbjct: 397 DALRSCCLCG*EYNGT 444
>gb|CA009582.1|CA009582 HU14I15r HU Hordeum vulgare subsp. vulgare cDNA clone HU14I15
5-PRIME, mRNA sequence
Length = 668
Score = 235 bits (507), Expect(2) = 2e-063
Identities = 96/114 (84%), Positives = 105/114 (92%)
Frame = +3 / +1
Query: 3 FDARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGS 182
FDARRKP N+GK+I AL+ LCIF LKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGS
Sbjct: 298 FDARRKPHNIGKVIVALLFLALCIFVLKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGS 477
Query: 183 HAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
HA+LRLL+D+YRVTIVDNLSRGN GAVKVLQ LFP+P RLQFI+ADLGDQKSV+
Sbjct: 478 HASLRLLKDNYRVTIVDNLSRGNKGAVKVLQELFPEPXRLQFIYADLGDQKSVN 639
Score = 204 bits (441), Expect(2) = 1e-054
Identities = 88/113 (77%), Positives = 92/113 (81%)
Frame = -1 / -1
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
TDF SPRSA MN SL G GN RTFTAP+FPL+RLSTMVTR*LS S ACEPI*PAP
Sbjct: 635 TDF*SPRSA*MN*SLXGSGNNSWRTFTAPLFPLERLSTMVTR*LSFNSRNEACEPI*PAP 456
Query: 160 PVTKTWVTPGSWRENTLLPPKPGDCFRANIQSVVRTRAAMIFPTLRGFRLASK 2
PVTKTWVTPGSWRENTLLPPKPGDCFR NIQS ++RA M FP L G RLASK
Sbjct: 455 PVTKTWVTPGSWRENTLLPPKPGDCFRTNIQSAKKSRATMTFPILCGLRLASK 297
Score = 182 bits (393), Expect(2) = 5e-047
Identities = 82/113 (72%), Positives = 92/113 (81%)
Frame = -3 / -3
Query: 341 YRFLVPQISKDEL*STRLRK*ALQNLYCSHISSR*IIHNGNSIAIL*EP*SGM*TNITSS 162
+RFL+P+I DEL S RLRK L+NLYCS ISSR I+HNGNSI I * P* GM*TNI SS
Sbjct: 636 HRFLIPKICIDELKSXRLRKQFLENLYCSFISSRKIVHNGNSIVIF**P**GM*TNIASS 457
Query: 161 SCY*DVGNTRFMARKHTIATKTRRLLQSKYTECCEDQGCNDFSNITRLPPCIK 3
SC+*D+GN RFMARKHTIATKTRRLLQ+KYTEC E+QG NDF NI R P I+
Sbjct: 456 SCH*DMGNPRFMARKHTIATKTRRLLQNKYTEC*EEQGHNDFPNIVRFTPSIE 298
Score = 177 bits (380), Expect = 5e-044
Identities = 70/103 (67%), Positives = 80/103 (77%)
Frame = +2 / +3
Query: 32 WKNHCSPGPHNTLYICSEAISWFWWQ*CVFSP*TWCYPRLSNRRSWLYWFTCRFKALTG* 211
W++HC P +TLYICSEAISWFWWQ*CVFSP*TW YP LS+RRSWLYWFTC A+ *
Sbjct: 327 WESHCGPALLSTLYICSEAISWFWWQ*CVFSP*TWGYPCLSDRRSWLYWFTCLITAIKR* 506
Query: 212 LSSYHCG*SI*RKYGSSKGSAGLISAAW*TTIHLC*SGGPEIC 340
LSSYHCG S *RK SSKGS ++S A T++HLC S G +IC
Sbjct: 507 LSSYHCGQSF*RK*RSSKGSPRIVSGAXETSVHLCRSWGSKIC 635
Score = 142 bits (304), Expect(2) = 1e-034
Identities = 70/113 (61%), Positives = 75/113 (66%)
Frame = -2 / -2
Query: 339 QISGPPDQQR*IVVYQAAEISPAEPLLLPYFL*IDYPQW*LDSYPVRALKRHVNQYNQXX 160
QI P D R* V QA E EPLLL YFL* D PQW*LDSY + A+ RHVNQY+Q
Sbjct: 634 QIFDPQDLHR*TEVSQAPETILGEPLLLLYFL*KDCPQW*LDSYLLIAVMRHVNQYSQLL 455
Query: 159 XXXXRG*HQVHGEKTHYCHQNQEIASEQIYRVL*GPGLQ*FFQHYEASALHQS 1
G* QVHGEKTHYCHQNQEIASEQIYRVL G Q* Q+ A H+S
Sbjct: 454 LSLRHG*PQVHGEKTHYCHQNQEIASEQIYRVLRRAGPQ*LSQYCAVYA*HRS 296
Score = 108 bits (231), Expect = 2e-023
Identities = 51/75 (68%), Positives = 58/75 (77%)
Frame = +1 / +2
Query: 115 CFLAMNLVLPTS**QEELVILVHMPL*GSYRIAIELPLWIIYLEEIWEQ*RFCRAYFRSL 294
CFLAMNL LP S**QEEL ILVHMP G +I IELPLW I+LEEI EQ*RF + FRSL
Sbjct: 410 CFLAMNLGLPMS**QEELAILVHMPHYGY*KITIELPLWTIFLEEIKEQ*RFSKNCFRSL 589
Query: 295 VDYNSSLLIWGTRNL 339
D++SS+ I G +NL
Sbjct: 590 XDFSSSMQILGIKNL 634
Score = 28.1 bits (55), Expect(2) = 1e-054
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -1 / -2
Query: 469 HQMHFQQISC 440
HQMHFQ+ISC
Sbjct: 667 HQMHFQKISC 638
Score = 27.2 bits (53), Expect(2) = 2e-063
Identities = 10/11 (90%), Positives = 11/11 (100%)
Frame = +1 / +1
Query: 436 VNKIFAENAFD 468
VNKIF+ENAFD
Sbjct: 634 VNKIFSENAFD 666
Score = 24.4 bits (47), Expect(2) = 5e-047
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = -2 / -3
Query: 468 IKCIFSKYLV 439
IKCIF KYLV
Sbjct: 666 IKCIFRKYLV 637
Score = 24.0 bits (46), Expect(2) = 1e-034
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = -3 / -1
Query: 470 ASNAFSANILLT 435
ASNAFS NIL T
Sbjct: 668 ASNAFSENILFT 633
>gb|BG300460.1|BG300460 HVSMEb0017C05f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0017C05f, mRNA sequence
Length = 871
Score = 187 bits (402), Expect(3) = 2e-062
Identities = 78/95 (82%), Positives = 84/95 (88%)
Frame = +3 / +3
Query: 3 FDARRKPRNVGKIIAALVLTTLCIFALKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGS 182
FDARRKP N+GK+I AL+ LCIF LKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGS
Sbjct: 222 FDARRKPHNIGKVIVALLFLALCIFVLKQSPGFGGNSVFSRHEPGVTHVLVTGGAGYIGS 401
Query: 183 HAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFP 287
HA+LRLL+D+YRVTIVDNLSRGN GAVKV L P
Sbjct: 402 HASLRLLKDNYRVTIVDNLSRGN*GAVKVPP*LVP 506
Score = 174 bits (374), Expect(3) = 1e-053
Identities = 73/89 (82%), Positives = 75/89 (84%)
Frame = -1 / -1
Query: 268 TFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAPPVTKTWVTPGSWRENTLLPPKPGD 89
TFTAP FPLDRLSTMVTR*LS S ACEPI*PAPPVTKTWVTPGSWRENTLLPPKPGD
Sbjct: 487 TFTAP*FPLDRLSTMVTR*LSFNSRNEACEPI*PAPPVTKTWVTPGSWRENTLLPPKPGD 308
Query: 88 CFRANIQSVVRTRAAMIFPTLRGFRLASK 2
CFR NIQS ++RA M FP L G RLASK
Sbjct: 307 CFRTNIQSAKKSRATMTFPILCGLRLASK 221
Score = 154 bits (330), Expect(2) = 3e-040
Identities = 59/81 (72%), Positives = 66/81 (81%)
Frame = +2 / +2
Query: 32 WKNHCSPGPHNTLYICSEAISWFWWQ*CVFSP*TWCYPRLSNRRSWLYWFTCRFKALTG* 211
W++HC P +TLYICSEAISWFWWQ*CVFSP*TW YP LS+RRSWLYWFTC A+ *
Sbjct: 251 WESHCGPALLSTLYICSEAISWFWWQ*CVFSP*TWGYPCLSDRRSWLYWFTCLITAIKR* 430
Query: 212 LSSYHCG*SI*RKYGSSKGSA 274
LSSYHCG S+*RK SSK S+
Sbjct: 431 LSSYHCGQSV*RKLRSSKSSS 493
Score = 152 bits (326), Expect(3) = 5e-045
Identities = 67/90 (74%), Positives = 74/90 (82%)
Frame = -3 / -3
Query: 272 QNLYCSHISSR*IIHNGNSIAIL*EP*SGM*TNITSSSCY*DVGNTRFMARKHTIATKTR 93
+N YCS ISSR I+HNGNSI I * P* GM*TNI SSSC+*D+GN RFMARKHTIATKTR
Sbjct: 491 RNFYCSLISSRQIVHNGNSIVIF**P**GM*TNIASSSCH*DMGNPRFMARKHTIATKTR 312
Query: 92 RLLQSKYTECCEDQGCNDFSNITRLPPCIK 3
RLLQ+KYTEC E+QG NDF NI R P I+
Sbjct: 311 RLLQNKYTEC*EEQGHNDFPNIVRFTPSIE 222
Score = 121 bits (259), Expect(2) = 2e-031
Identities = 58/90 (64%), Positives = 63/90 (70%)
Frame = -2 / -2
Query: 270 EPLLLPYFL*IDYPQW*LDSYPVRALKRHVNQYNQXXXXXXRG*HQVHGEKTHYCHQNQE 91
E LLL FL* D PQW*LDSY + A+ RHVNQY+Q G* QVHGEKTHYCHQNQE
Sbjct: 489 ELLLLLNFL*TDCPQW*LDSYLLIAVMRHVNQYSQLLLSLRHG*PQVHGEKTHYCHQNQE 310
Query: 90 IASEQIYRVL*GPGLQ*FFQHYEASALHQS 1
IASEQIYRVL G Q* Q+ A H+S
Sbjct: 309 IASEQIYRVLRRAGPQ*LSQYCAVYA*HRS 220
Score = 50.1 bits (103), Expect(3) = 2e-062
Identities = 19/27 (70%), Positives = 23/27 (85%)
Frame = +3 / +1
Query: 264 KVLQGLFPQPGRLQFIFADLGDQKSVS 344
K L FP+PGRLQFI++DLGDQKSV+
Sbjct: 484 KFLHNWFPEPGRLQFIYSDLGDQKSVN 564
Score = 40.5 bits (82), Expect(3) = 2e-062
Identities = 17/29 (58%), Positives = 19/29 (65%)
Frame = +1 / +1
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VNKIF ENAFDAVMHF G ++ P
Sbjct: 559 VNKIFPENAFDAVMHFRNCCLCGWKSIGP 645
Score = 37.7 bits (76), Expect(3) = 1e-053
Identities = 17/26 (65%), Positives = 18/26 (69%)
Frame = -1 / -3
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTF 263
TDF SPRS MN SLPG GN+ R F
Sbjct: 560 TDF*SPRSE*MNWSLPGSGNQLWRNF 483
Score = 36.3 bits (73), Expect(3) = 1e-053
Identities = 13/14 (92%), Positives = 14/14 (100%)
Frame = -1 / -1
Query: 481 SASQHQMHFQQISC 440
SASQHQMHFQ+ISC
Sbjct: 604 SASQHQMHFQEISC 563
Score = 35.9 bits (72), Expect(3) = 5e-045
Identities = 13/15 (86%), Positives = 14/15 (93%)
Frame = -2 / -2
Query: 483 KVHHSIKCIFSKYLV 439
+VHHSIKCIF KYLV
Sbjct: 606 EVHHSIKCIFRKYLV 562
Score = 35.0 bits (70), Expect(2) = 2e-031
Identities = 14/16 (87%), Positives = 14/16 (87%)
Frame = -3 / -3
Query: 482 KCITASNAFSANILLT 435
KCITASNAFS NIL T
Sbjct: 605 KCITASNAFSGNILFT 558
Score = 30.9 bits (61), Expect(3) = 5e-045
Identities = 14/26 (53%), Positives = 19/26 (73%)
Frame = -3 / -2
Query: 341 YRFLVPQISKDEL*STRLRK*ALQNL 264
+RFL+PQI DEL S RLR+ ++ L
Sbjct: 561 HRFLIPQI*IDELESPRLREPIMEEL 484
Score = 30.4 bits (60), Expect(2) = 3e-040
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +2 / +3
Query: 254 GSSKGSAGLISAAW*TTIHLC*SGGPEIC 340
G+ K L+ AW T +HL SGG +IC
Sbjct: 474 GAVKVPP*LVPGAWETPVHLFRSGGSKIC 560
>gb|BF255654.2|BF255654 HVSMEf0007H02f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0007H02f, mRNA sequence
Length = 841
Score = 156 bits (335), Expect(3) = 2e-055
Identities = 67/78 (85%), Positives = 71/78 (91%)
Frame = +3 / +1
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQ 290
SVFS HE GVTHVLVTGGAGYIGSHAALRLL DS+RVTIVDNLSRGN+GAVKVLQ LFP
Sbjct: 100 SVFSIHELGVTHVLVTGGAGYIGSHAALRLLNDSFRVTIVDNLSRGNIGAVKVLQNLFPD 279
Query: 291 PGRLQFIFADLGDQKSVS 344
PGRLQFI ADLGD K+V+
Sbjct: 280 PGRLQFIHADLGDAKAVN 333
Score = 120 bits (257), Expect(2) = 9e-034
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = -1 / -3
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
T F SPRSA MNC+ PG GNK RT TAPIFPL+RLSTMVT S S +AAC+P+*PAP
Sbjct: 329 TAFASPRSACMNCNRPGSGNKF*RTLTAPIFPLERLSTMVTLKESLSSRRAACDPM*PAP 150
Query: 160 PVTKTWVTPGSWRENTL 110
PVT T VTP SW ENTL
Sbjct: 149 PVTNT*VTPSSWIENTL 99
Score = 67.5 bits (141), Expect(3) = 2e-055
Identities = 28/32 (87%), Positives = 29/32 (90%)
Frame = +1 / +1
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
A VN+IFAENAFDAVMHFAA AYVGESTLEP
Sbjct: 319 AKAVNRIFAENAFDAVMHFAAVAYVGESTLEP 414
Score = 42.3 bits (86), Expect(2) = 9e-034
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = -1 / -1
Query: 511 CSLPHKXQLQSASQHQMHFQQISC* 437
CS PHK + QSA QHQMHF +I C*
Sbjct: 403 CSRPHKQRQQSA*QHQMHFLRIFC* 329
Score = 30.4 bits (60), Expect(3) = 2e-055
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +3 / +1
Query: 15 RKPRNVGKIIAALVLTTLCIFALKQSP 95
RK R + KII +LT +C+ L Q P
Sbjct: 1 RKSRYLSKIIMVALLTAMCVVMLTQPP 81
>gb|BJ454539.1|BJ454539 BJ454539 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak24n03 5', mRNA sequence
Length = 629
Score = 165 bits (355), Expect(2) = 5e-054
Identities = 68/76 (89%), Positives = 74/76 (97%)
Frame = +3 / +1
Query: 117 FSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPG 296
FSRHEPGVTHVLVTGGAGYIGSHA+LRLL+D+YRVTIVDNLSRGN GAVKVLQ LFP+PG
Sbjct: 40 FSRHEPGVTHVLVTGGAGYIGSHASLRLLKDNYRVTIVDNLSRGNKGAVKVLQELFPEPG 219
Query: 297 RLQFIFADLGDQKSVS 344
RLQFI+ADLGDQKSV+
Sbjct: 220 RLQFIYADLGDQKSVN 267
Score = 142 bits (305), Expect(2) = 3e-045
Identities = 61/75 (81%), Positives = 63/75 (84%)
Frame = -1 / -1
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
TDF SPRSA MN SLPG GN RTFTAP+FPL+RLSTMVTR*LS S ACEPI*PAP
Sbjct: 263 TDF*SPRSA*MN*SLPGSGNNSWRTFTAPLFPLERLSTMVTR*LSFNSRNEACEPI*PAP 84
Query: 160 PVTKTWVTPGSWREN 116
PVTKTWVTPGSWREN
Sbjct: 83 PVTKTWVTPGSWREN 39
Score = 120 bits (257), Expect(2) = 3e-027
Identities = 50/75 (66%), Positives = 57/75 (76%)
Frame = +2 / +3
Query: 116 VFSP*TWCYPRLSNRRSWLYWFTCRFKALTG*LSSYHCG*SI*RKYGSSKGSAGLISAAW 295
VFSP*TW YP LS+RRSWLYWFTC A+ *LSSYHCG S *RK SSKGS ++S AW
Sbjct: 39 VFSP*TWGYPCLSDRRSWLYWFTCLITAIKR*LSSYHCGQSF*RK*RSSKGSPRIVSGAW 218
Query: 296 *TTIHLC*SGGPEIC 340
T++HLC S G +IC
Sbjct: 219 ETSVHLCRSWGSKIC 263
Score = 116 bits (248), Expect(2) = 1e-036
Identities = 54/75 (72%), Positives = 61/75 (81%)
Frame = -3 / -3
Query: 341 YRFLVPQISKDEL*STRLRK*ALQNLYCSHISSR*IIHNGNSIAIL*EP*SGM*TNITSS 162
+RFL+P+I DEL S RLRK L+NLYCS ISSR I+HNGNSI I * P* GM*TNI SS
Sbjct: 264 HRFLIPKICIDELKSPRLRKQFLENLYCSFISSRKIVHNGNSIVIF**P**GM*TNIASS 85
Query: 161 SCY*DVGNTRFMARK 117
SC+*D+GN RFMARK
Sbjct: 84 SCH*DMGNPRFMARK 40
Score = 103 bits (220), Expect = 6e-022
Identities = 50/74 (67%), Positives = 57/74 (77%)
Frame = +1 / +2
Query: 118 FLAMNLVLPTS**QEELVILVHMPL*GSYRIAIELPLWIIYLEEIWEQ*RFCRAYFRSLV 297
FLAMNL LP S**QEEL ILVHMP G +I IELPLW I+LEEI EQ*RF + FRSL
Sbjct: 41 FLAMNLGLPMS**QEELAILVHMPHYGY*KITIELPLWTIFLEEIKEQ*RFSKNCFRSLG 220
Query: 298 DYNSSLLIWGTRNL 339
D++SS+ I G +NL
Sbjct: 221 DFSSSMQILGIKNL 262
Score = 91.8 bits (194), Expect(2) = 7e-029
Identities = 46/79 (58%), Positives = 49/79 (62%)
Frame = -2 / -2
Query: 339 QISGPPDQQR*IVVYQAAEISPAEPLLLPYFL*IDYPQW*LDSYPVRALKRHVNQYNQXX 160
QI P D R* V QA E EPLLL YFL* D PQW*LDSY + A+ RHVNQY+Q
Sbjct: 262 QIFDPQDLHR*TEVSQAPETILGEPLLLLYFL*KDCPQW*LDSYLLIAVMRHVNQYSQLL 83
Query: 159 XXXXRG*HQVHGEKTHYCH 103
G* QVHGEKT H
Sbjct: 82 LSLRHG*PQVHGEKTAVVH 26
Score = 65.2 bits (136), Expect(2) = 5e-054
Identities = 26/29 (89%), Positives = 28/29 (96%)
Frame = +1 / +1
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VNKIF+ENAFDAVMHFAA AYVGEST+EP
Sbjct: 262 VNKIFSENAFDAVMHFAAVAYVGESTMEP 348
Score = 58.8 bits (122), Expect(2) = 3e-045
Identities = 21/28 (75%), Positives = 23/28 (82%)
Frame = -2 / -3
Query: 522 GFQCALSHISXSCKVHHSIKCIFSKYLV 439
GF C L+HI SC+VHHSIKCIF KYLV
Sbjct: 348 GFHCTLTHIGNSCEVHHSIKCIFRKYLV 265
Score = 56.1 bits (116), Expect(2) = 1e-036
Identities = 21/28 (75%), Positives = 25/28 (89%)
Frame = -1 / -2
Query: 523 GVPMCSLPHKXQLQSASQHQMHFQQISC 440
GVP+ S PH+ QL+SASQHQMHFQ+ISC
Sbjct: 349 GVPLYSHPHRQQLRSASQHQMHFQKISC 266
Score = 54.7 bits (113), Expect(2) = 7e-029
Identities = 25/29 (86%), Positives = 25/29 (86%)
Frame = -3 / -1
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
GS VLSPT*A AAKCITASNAFS NIL T
Sbjct: 347 GSIVLSPT*ATAAKCITASNAFSENILFT 261
Score = 21.7 bits (41), Expect(2) = 3e-027
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +3 / +3
Query: 474 DALCSXXLCGREHIGT 521
DAL S LCG E+ GT
Sbjct: 300 DALRSCCLCG*EYNGT 347
>gb|BJ468853.1|BJ468853 BJ468853 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd27i15 5', mRNA sequence
Length = 561
Score = 156 bits (335), Expect(3) = 1e-053
Identities = 67/78 (85%), Positives = 71/78 (91%)
Frame = +3 / +3
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQ 290
SVFS HE GVTHVLVTGGAGYIGSHAALRLL DS+RVTIVDNLSRGN+GAVKVLQ LFP
Sbjct: 258 SVFSIHELGVTHVLVTGGAGYIGSHAALRLLNDSFRVTIVDNLSRGNIGAVKVLQNLFPD 437
Query: 291 PGRLQFIFADLGDQKSVS 344
PGRLQFI ADLGD K+V+
Sbjct: 438 PGRLQFIHADLGDAKAVN 491
Score = 120 bits (257), Expect(2) = 9e-034
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = -1 / -3
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
T F SPRSA MNC+ PG GNK RT TAPIFPL+RLSTMVT S S +AAC+P+*PAP
Sbjct: 487 TAFASPRSACMNCNRPGSGNKF*RTLTAPIFPLERLSTMVTLKESLSSRRAACDPM*PAP 308
Query: 160 PVTKTWVTPGSWRENTL 110
PVT T VTP SW ENTL
Sbjct: 307 PVTNT*VTPSSWIENTL 257
Score = 57.9 bits (120), Expect(3) = 1e-053
Identities = 24/28 (85%), Positives = 25/28 (89%)
Frame = +1 / +3
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGES 510
A VN+IFAENAFDAVMHFAA AYVGES
Sbjct: 477 AKAVNRIFAENAFDAVMHFAAVAYVGES 560
Score = 42.3 bits (86), Expect(2) = 9e-034
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = -1 / -1
Query: 511 CSLPHKXQLQSASQHQMHFQQISC* 437
CS PHK + QSA QHQMHF +I C*
Sbjct: 561 CSRPHKQRQQSA*QHQMHFLRIFC* 487
Score = 33.6 bits (67), Expect(3) = 1e-053
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3 / +3
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +RK R + KII +LT +C+ L Q P
Sbjct: 150 DPKRKSRYLSKIIMVALLTAMCVVMLTQPP 239
>gb|AV922700.1|AV922700 AV922700 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd2a02 5', mRNA sequence
Length = 608
Score = 156 bits (335), Expect(4) = 2e-052
Identities = 67/78 (85%), Positives = 71/78 (91%)
Frame = +3 / +2
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQ 290
SVFS HE GVTHVLVTGGAGYIGSHAALRLL DS+RVTIVDNLSRGN+GAVKVLQ LFP
Sbjct: 263 SVFSIHELGVTHVLVTGGAGYIGSHAALRLLNDSFRVTIVDNLSRGNIGAVKVLQNLFPD 442
Query: 291 PGRLQFIFADLGDQKSVS 344
PGRLQFI ADLGD K+V+
Sbjct: 443 PGRLQFIHADLGDAKAVN 496
Score = 120 bits (257), Expect(3) = 2e-032
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = -1 / -3
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
T F SPRSA MNC+ PG GNK RT TAPIFPL+RLSTMVT S S +AAC+P+*PAP
Sbjct: 492 TAFASPRSACMNCNRPGSGNKF*RTLTAPIFPLERLSTMVTLKESLSSRRAACDPM*PAP 313
Query: 160 PVTKTWVTPGSWRENTL 110
PVT T VTP SW ENTL
Sbjct: 312 PVTNT*VTPSSWIENTL 262
Score = 44.1 bits (90), Expect(4) = 2e-052
Identities = 18/26 (69%), Positives = 19/26 (73%)
Frame = +1 / +2
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVG 504
A VN+IFAENAFDAVMHFA G
Sbjct: 482 AKAVNRIFAENAFDAVMHFACRCLCG 559
Score = 33.6 bits (67), Expect(4) = 2e-052
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +RK R + KII +LT +C+ L Q P
Sbjct: 155 DPKRKSRYLSKIIMVALLTAMCVVMLTQPP 244
Score = 29.9 bits (59), Expect(3) = 2e-032
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -2 / -2
Query: 495 SXSCKVHHSIKCIFSKYLVD 436
+ + KVH SIKCIF +Y V+
Sbjct: 550 ATASKVHDSIKCIFCEYSVN 491
Score = 27.6 bits (54), Expect(4) = 2e-052
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +1 / +3
Query: 487 AXAYVGESTLEP 522
A AYVGESTLEP
Sbjct: 543 AVAYVGESTLEP 578
Score = 25.8 bits (50), Expect(3) = 2e-032
Identities = 11/14 (78%), Positives = 13/14 (92%)
Frame = -3 / -2
Query: 521 GSNVLSPT*AXAAK 480
GS+VLSPT*A A+K
Sbjct: 577 GSSVLSPT*ATASK 536
>gb|BQ472666.1|BQ472666 HB01N17r BC Hordeum vulgare subsp. vulgare cDNA clone HB01N17
5-PRIME, mRNA sequence
Length = 521
Score = 156 bits (335), Expect(2) = 6e-052
Identities = 67/78 (85%), Positives = 71/78 (91%)
Frame = +3 / +3
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQ 290
SVFS HE GVTHVLVTGGAGYIGSHAALRLL DS+RVTIVDNLSRGN+GAVKVLQ LFP
Sbjct: 39 SVFSIHELGVTHVLVTGGAGYIGSHAALRLLNDSFRVTIVDNLSRGNIGAVKVLQNLFPD 218
Query: 291 PGRLQFIFADLGDQKSVS 344
PGRLQFI ADLGD K+V+
Sbjct: 219 PGRLQFIHADLGDAKAVN 272
Score = 120 bits (257), Expect(2) = 9e-034
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = -1 / -2
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
T F SPRSA MNC+ PG GNK RT TAPIFPL+RLSTMVT S S +AAC+P+*PAP
Sbjct: 268 TAFASPRSACMNCNRPGSGNKF*RTLTAPIFPLERLSTMVTLKESLSSRRAACDPM*PAP 89
Query: 160 PVTKTWVTPGSWRENTL 110
PVT T VTP SW ENTL
Sbjct: 88 PVTNT*VTPSSWIENTL 38
Score = 67.5 bits (141), Expect(2) = 6e-052
Identities = 28/32 (87%), Positives = 29/32 (90%)
Frame = +1 / +3
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
A VN+IFAENAFDAVMHFAA AYVGESTLEP
Sbjct: 258 AKAVNRIFAENAFDAVMHFAAVAYVGESTLEP 353
Score = 42.3 bits (86), Expect(2) = 9e-034
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = -1 / -3
Query: 511 CSLPHKXQLQSASQHQMHFQQISC* 437
CS PHK + QSA QHQMHF +I C*
Sbjct: 342 CSRPHKQRQQSA*QHQMHFLRIFC* 268
>gb|CA027499.1|CA027499 HZ59C02r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ59C02
5-PRIME, mRNA sequence
Length = 405
Score = 152 bits (326), Expect(2) = 7e-051
Identities = 64/75 (85%), Positives = 69/75 (92%)
Frame = +3 / +2
Query: 120 SRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGR 299
+RHE GVTHVLVTGGAGYIGSHAALRLL DS+RVTIVDNLSRGN+GAVKVLQ LFP PGR
Sbjct: 5 TRHELGVTHVLVTGGAGYIGSHAALRLLNDSFRVTIVDNLSRGNIGAVKVLQNLFPDPGR 184
Query: 300 LQFIFADLGDQKSVS 344
LQFI ADLGD K+V+
Sbjct: 185 LQFIHADLGDAKAVN 229
Score = 107 bits (229), Expect(2) = 4e-030
Identities = 49/71 (69%), Positives = 54/71 (76%)
Frame = -1 / -1
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
T F SPRSA MNC+ PG GNK RT TAPIFPL+RLSTMVT S S +AAC+P+*PAP
Sbjct: 225 TAFASPRSACMNCNRPGSGNKF*RTLTAPIFPLERLSTMVTLKESLSSRRAACDPM*PAP 46
Query: 160 PVTKTWVTPGS 128
PVT T VTP S
Sbjct: 45 PVTNT*VTPSS 13
Score = 67.5 bits (141), Expect(2) = 7e-051
Identities = 28/32 (87%), Positives = 29/32 (90%)
Frame = +1 / +2
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
A VN+IFAENAFDAVMHFAA AYVGESTLEP
Sbjct: 215 AKAVNRIFAENAFDAVMHFAAVAYVGESTLEP 310
Score = 42.3 bits (86), Expect(2) = 4e-030
Identities = 17/25 (68%), Positives = 19/25 (76%)
Frame = -1 / -2
Query: 511 CSLPHKXQLQSASQHQMHFQQISC* 437
CS PHK + QSA QHQMHF +I C*
Sbjct: 299 CSRPHKQRQQSA*QHQMHFLRIFC* 225
>gb|BJ462697.1|BJ462697 BJ462697 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags29p06 5', mRNA sequence
Length = 590
Score = 156 bits (335), Expect(3) = 5e-049
Identities = 67/78 (85%), Positives = 71/78 (91%)
Frame = +3 / +2
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQ 290
SVFS HE GVTHVLVTGGAGYIGSHAALRLL DS+RVTIVDNLSRGN+GAVKVLQ LFP
Sbjct: 269 SVFSIHELGVTHVLVTGGAGYIGSHAALRLLNDSFRVTIVDNLSRGNIGAVKVLQNLFPD 448
Query: 291 PGRLQFIFADLGDQKSVS 344
PGRLQFI ADLGD K+V+
Sbjct: 449 PGRLQFIHADLGDAKAVN 502
Score = 120 bits (257), Expect(3) = 2e-032
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = -1 / -3
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
T F SPRSA MNC+ PG GNK RT TAPIFPL+RLSTMVT S S +AAC+P+*PAP
Sbjct: 498 TAFASPRSACMNCNRPGSGNKF*RTLTAPIFPLERLSTMVTLKESLSSRRAACDPM*PAP 319
Query: 160 PVTKTWVTPGSWRENTL 110
PVT T VTP SW ENTL
Sbjct: 318 PVTNT*VTPSSWIENTL 268
Score = 42.3 bits (86), Expect(3) = 5e-049
Identities = 17/26 (65%), Positives = 18/26 (69%)
Frame = +1 / +2
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVG 504
A VN+IFAENAFDAVMHF G
Sbjct: 488 AKAVNRIFAENAFDAVMHFCCRCLCG 565
Score = 33.6 bits (67), Expect(3) = 5e-049
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +RK R + KII +LT +C+ L Q P
Sbjct: 161 DPKRKSRYLSKIIMVALLTAMCVVMLTQPP 250
Score = 30.4 bits (60), Expect(3) = 2e-032
Identities = 11/20 (55%), Positives = 15/20 (75%)
Frame = -2 / -2
Query: 495 SXSCKVHHSIKCIFSKYLVD 436
+ + KVH SIKCIF +Y V+
Sbjct: 556 ATAAKVHDSIKCIFCEYSVN 497
Score = 25.4 bits (49), Expect(3) = 2e-032
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = -3 / -2
Query: 521 GSNVLSPT*AXAAK 480
GS+VL PT*A AAK
Sbjct: 583 GSSVLXPT*ATAAK 542
>gb|BU999595.1|BU999595 HI14P19r HI Hordeum vulgare subsp. vulgare cDNA clone HI14P19
5-PRIME, mRNA sequence
Length = 575
Score = 156 bits (335), Expect(3) = 9e-048
Identities = 67/78 (85%), Positives = 71/78 (91%)
Frame = +3 / +1
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQ 290
SVFS HE GVTHVLVTGGAGYIGSHAALRLL DS+RVTIVDNLSRGN+GAVKVLQ LFP
Sbjct: 301 SVFSIHELGVTHVLVTGGAGYIGSHAALRLLNDSFRVTIVDNLSRGNIGAVKVLQNLFPD 480
Query: 291 PGRLQFIFADLGDQKSVS 344
PGRLQFI ADLGD K+V+
Sbjct: 481 PGRLQFIHADLGDAKAVN 534
Score = 120 bits (257), Expect(2) = 3e-029
Identities = 54/77 (70%), Positives = 59/77 (76%)
Frame = -1 / -1
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
T F SPRSA MNC+ PG GNK RT TAPIFPL+RLSTMVT S S +AAC+P+*PAP
Sbjct: 530 TAFASPRSACMNCNRPGSGNKF*RTLTAPIFPLERLSTMVTLKESLSSRRAACDPM*PAP 351
Query: 160 PVTKTWVTPGSWRENTL 110
PVT T VTP SW ENTL
Sbjct: 350 PVTNT*VTPSSWIENTL 300
Score = 38.2 bits (77), Expect(3) = 9e-048
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = +1 / +1
Query: 427 ASQVNKIFAENAFDAVMH 480
A VN+IFAENAFDAVMH
Sbjct: 520 AKAVNRIFAENAFDAVMH 573
Score = 33.6 bits (67), Expect(3) = 9e-048
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3 / +1
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +RK R + KII +LT +C+ L Q P
Sbjct: 193 DPKRKSRYLSKIIMVALLTAMCVVMLTQPP 282
Score = 27.2 bits (53), Expect(2) = 3e-029
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = -2 / -3
Query: 480 VHHSIKCIFSKYLVD 436
VH SIKCIF +Y V+
Sbjct: 573 VHDSIKCIFCEYSVN 529
>gb|BM374688.1|BM374688 EBma05_SQ002_H10_R maternal, 12 DPA, no treatment, cv Optic, EBma05
Hordeum vulgare subsp. vulgare cDNA clone
EBma05_SQ002_H10 5', mRNA sequence
Length = 564
Score = 153 bits (328), Expect(2) = 7e-041
Identities = 66/78 (84%), Positives = 70/78 (89%)
Frame = +3 / +3
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQ 290
SVFS HE GVTHVLVTGGAGYIGSHAALRLL DS+RVTIVDNLSRGN+GAVKVLQ LFP
Sbjct: 318 SVFSIHELGVTHVLVTGGAGYIGSHAALRLLNDSFRVTIVDNLSRGNIGAVKVLQNLFPD 497
Query: 291 PGRLQFIFADLGDQKSVS 344
P RLQFI ADLGD K+V+
Sbjct: 498 PXRLQFIHADLGDAKAVN 551
Score = 116 bits (248), Expect = 9e-026
Identities = 53/77 (68%), Positives = 58/77 (75%)
Frame = -1 / -3
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAP 161
T F SPRSA MNC+ G GNK RT TAPIFPL+RLSTMVT S S +AAC+P+*PAP
Sbjct: 547 TAFASPRSACMNCNRXGSGNKF*RTLTAPIFPLERLSTMVTLKESLSSRRAACDPM*PAP 368
Query: 160 PVTKTWVTPGSWRENTL 110
PVT T VTP SW ENTL
Sbjct: 367 PVTNT*VTPSSWIENTL 317
Score = 33.6 bits (67), Expect(2) = 7e-041
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3 / +3
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +RK R + KII +LT +C+ L Q P
Sbjct: 210 DPKRKSRYLSKIIMVALLTAMCVVMLTQPP 299
>gb|BI947860.1|BI947860 HVSMEl0007D23f Hordeum vulgare spike EST library HVcDNA0012
(Fusarium infected) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEl0007D23f, mRNA sequence
Length = 849
Score = 132 bits (283), Expect(3) = 4e-039
Identities = 59/78 (75%), Positives = 63/78 (80%)
Frame = +3 / +3
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQ 290
SVFS HE GVTHV VTGGAGYIGSHAALRLL DS+ VTIVDNLSRGN+GAVK LQ LFP
Sbjct: 258 SVFSIHELGVTHVWVTGGAGYIGSHAALRLLNDSFTVTIVDNLSRGNIGAVKALQNLFPD 437
Query: 291 PGRLQFIFADLGDQKSVS 344
GR Q I ADL D +V+
Sbjct: 438 TGREQLIHADLRDAYAVN 491
Score = 105 bits (224), Expect = 2e-022
Identities = 49/73 (67%), Positives = 54/73 (73%)
Frame = -1 / -3
Query: 328 SPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI*PAPPVTK 149
S RSA M+CS P GNK R TAPIFPL+RLSTMVT S S +AAC+P+*PAPPVT
Sbjct: 475 SRRSACMSCSRPVSGNKF*RALTAPIFPLERLSTMVTVKESLSSRRAACDPM*PAPPVTH 296
Query: 148 TWVTPGSWRENTL 110
T VTP SW ENTL
Sbjct: 295 T*VTPSSWIENTL 257
Score = 39.1 bits (79), Expect(3) = 4e-039
Identities = 17/32 (53%), Positives = 21/32 (65%)
Frame = +1 / +3
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
A VN++F + A AV+HF A A GESTL P
Sbjct: 477 AYAVNRLFTQQALGAVLHFDADANGGESTLGP 572
Score = 27.2 bits (53), Expect(3) = 4e-039
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +3 / +3
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +R + KII +LT +C+ L Q P
Sbjct: 150 DPKRGGGYLSKIIMVALLTAMCVLMLTQPP 239
>gb|BE195440.3|BE195440 HVSMEh0089K10f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0089K10f, mRNA sequence
Length = 988
Score = 116 bits (247), Expect(2) = 1e-038
Identities = 47/59 (79%), Positives = 53/59 (89%)
Frame = +3 / +1
Query: 168 GYIGSHAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
GYIGSHA LRLL D+YRVTIVDNLSRGNMGAV+ LQ LFP+PGRLQFI+ DLGD K+V+
Sbjct: 1 GYIGSHATLRLLTDNYRVTIVDNLSRGNMGAVRALQRLFPEPGRLQFIYTDLGDAKAVN 177
Score = 77.1 bits (162), Expect(2) = 2e-024
Identities = 34/56 (60%), Positives = 41/56 (73%)
Frame = -1 / -3
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAACEPI 173
T F SP+S +N SLPG GN R TAP+FPL+RLSTMVTR*LS + + ACEP+
Sbjct: 173 TAFASPKSVYIN*SLPGSGNSL*RALTAPMFPLERLSTMVTR*LSVRRRRVACEPM 6
Score = 63.4 bits (132), Expect(2) = 1e-038
Identities = 26/32 (81%), Positives = 28/32 (87%)
Frame = +1 / +1
Query: 427 ASQVNKIFAENAFDAVMHFAAXAYVGESTLEP 522
A VNK+F+ENAFDAVMHFAA AYVGEST EP
Sbjct: 163 AKAVNKMFSENAFDAVMHFAAVAYVGESTQEP 258
Score = 54.7 bits (113), Expect(2) = 2e-024
Identities = 25/29 (86%), Positives = 25/29 (86%)
Frame = -3 / -3
Query: 521 GSNVLSPT*AXAAKCITASNAFSANILLT 435
GS VLSPT*A AAKCITASNAFS NIL T
Sbjct: 257 GSCVLSPT*ATAAKCITASNAFSENILFT 171
>gb|BJ463647.1|BJ463647 BJ463647 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags32o13 5', mRNA sequence
Length = 533
Score = 108 bits (231), Expect(2) = 5e-037
Identities = 45/54 (83%), Positives = 51/54 (94%)
Frame = +3 / +1
Query: 183 HAALRLLQDSYRVTIVDNLSRGNMGAVKVLQGLFPQPGRLQFIFADLGDQKSVS 344
HA+LRLL+D+YRVTIVDNLSRGN GAVKVLQ LFP+PGRL FI+ADLGDQKSV+
Sbjct: 28 HASLRLLKDNYRVTIVDNLSRGNKGAVKVLQELFPEPGRLXFIYADLGDQKSVN 189
Score = 82.2 bits (173), Expect(2) = 3e-027
Identities = 38/52 (73%), Positives = 40/52 (76%)
Frame = -1 / -1
Query: 340 TDFWSPRSAKMNCSLPGCGNKPCRTFTAPIFPLDRLSTMVTR*LSCKSLKAA 185
TDF SPRSA MN SLPG GN RTFTAP+FPL+RLSTMVTR*LS S A
Sbjct: 185 TDF*SPRSA*MNXSLPGSGNNSWRTFTAPLFPLERLSTMVTR*LSFNSRNEA 30
Score = 76.2 bits (160), Expect(2) = 1e-024
Identities = 36/53 (67%), Positives = 41/53 (77%)
Frame = -3 / -3
Query: 341 YRFLVPQISKDEL*STRLRK*ALQNLYCSHISSR*IIHNGNSIAIL*EP*SGM 183
+RFL+P+I DEL S RLRK L+NLYCS ISSR I+HNGNSI I * P* GM
Sbjct: 186 HRFLIPKICIDELKSPRLRKQFLENLYCSFISSRKIVHNGNSIVIF**P**GM 28
Score = 65.2 bits (136), Expect(2) = 5e-037
Identities = 26/29 (89%), Positives = 28/29 (96%)
Frame = +1 / +1
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VNKIF+ENAFDAVMHFAA AYVGEST+EP
Sbjct: 184 VNKIFSENAFDAVMHFAAVAYVGESTMEP 270
Score = 58.8 bits (122), Expect(2) = 3e-027
Identities = 21/28 (75%), Positives = 23/28 (82%)
Frame = -2 / -3
Query: 522 GFQCALSHISXSCKVHHSIKCIFSKYLV 439
GF C L+HI SC+VHHSIKCIF KYLV
Sbjct: 270 GFHCTLTHIGNSCEVHHSIKCIFRKYLV 187
Score = 56.1 bits (116), Expect(2) = 1e-024
Identities = 21/28 (75%), Positives = 25/28 (89%)
Frame = -1 / -2
Query: 523 GVPMCSLPHKXQLQSASQHQMHFQQISC 440
GVP+ S PH+ QL+SASQHQMHFQ+ISC
Sbjct: 271 GVPLYSHPHRQQLRSASQHQMHFQKISC 188
>gb|BI952630.1|BI952630 HVSMEm0007D23f Hordeum vulgare green seedling EST library
HVcDNA0014 (Blumeria infected) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEm0007D23f, mRNA sequence
Length = 625
Score = 84.5 bits (178), Expect(3) = 1e-026
Identities = 36/42 (85%), Positives = 38/42 (90%)
Frame = +3 / +2
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDN 236
SVFS HE GVTHV VTGGAGYIGSHAAL+LL DS+RVTIVDN
Sbjct: 248 SVFSIHELGVTHVWVTGGAGYIGSHAALQLLNDSFRVTIVDN 373
Score = 45.1 bits (92), Expect(3) = 1e-026
Identities = 19/29 (65%), Positives = 22/29 (75%)
Frame = +3 / +3
Query: 237 LSRGNMGAVKVLQGLFPQPGRLQFIFADL 323
LSRGN+GA + LQ LFP GRLQ I A+L
Sbjct: 375 LSRGNIGADEALQNLFPDTGRLQLIHAEL 461
Score = 27.2 bits (53), Expect(3) = 1e-026
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +R + KII +LT +C+ L Q P
Sbjct: 140 DPKRGGGYLSKIIMVALLTAMCVLMLTQPP 229
>gb|CA027598.1|CA027598 HZ59G21r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ59G21
5-PRIME, mRNA sequence
Length = 367
Score = 100 bits (213), Expect(2) = 2e-025
Identities = 43/47 (91%), Positives = 44/47 (93%)
Frame = +3 / +2
Query: 111 SVFSRHEPGVTHVLVTGGAGYIGSHAALRLLQDSYRVTIVDNLSRGN 251
SVFS HE GVTHVLVTGGAGYIGSHAALRLL DS+RVTIVDNLSRGN
Sbjct: 227 SVFSIHELGVTHVLVTGGAGYIGSHAALRLLNDSFRVTIVDNLSRGN 367
Score = 33.6 bits (67), Expect(2) = 2e-025
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = +3 / +2
Query: 6 DARRKPRNVGKIIAALVLTTLCIFALKQSP 95
D +RK R + KII +LT +C+ L Q P
Sbjct: 119 DPKRKSRYLSKIIMVALLTAMCVVMLTQPP 208
>gb|BU974311.1|BU974311 HB27I16r BC Hordeum vulgare subsp. vulgare cDNA clone HB27I16
5-PRIME, mRNA sequence
Length = 450
Score = 65.2 bits (136), Expect(2) = 1e-022
Identities = 26/29 (89%), Positives = 28/29 (96%)
Frame = +1 / +2
Query: 436 VNKIFAENAFDAVMHFAAXAYVGESTLEP 522
VNKIF+ENAFDAVMHFAA AYVGEST+EP
Sbjct: 89 VNKIFSENAFDAVMHFAAVAYVGESTMEP 175
Score = 59.7 bits (124), Expect(2) = 1e-022
Identities = 24/28 (85%), Positives = 27/28 (96%)
Frame = +3 / +2
Query: 261 VKVLQGLFPQPGRLQFIFADLGDQKSVS 344
VKVLQ LFP+PGRLQFI+ADLGDQKSV+
Sbjct: 11 VKVLQELFPEPGRLQFIYADLGDQKSVN 94
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 253,060,920
Number of Sequences: 312970
Number of extensions: 4023623
Number of successful extensions: 58625
Number of sequences better than 1.0e-020: 24
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5255750517
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)