TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCA17a05.yg.2.1
(548 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV915881.1|AV915881 AV915881 K. Sato unpublished cDNA li... 132 3e-048
gb|AV915078.1|AV915078 AV915078 K. Sato unpublished cDNA li... 132 3e-044
gb|AV920212.1|AV920212 AV920212 K. Sato unpublished cDNA li... 131 3e-030
gb|AV833885.1|AV833885 AV833885 K. Sato unpublished cDNA li... 88 1e-028
>gb|AV915881.1|AV915881 AV915881 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags16g24 5', mRNA sequence
Length = 606
Score = 132 bits (282), Expect(2) = 3e-048
Identities = 46/54 (85%), Positives = 51/54 (94%)
Frame = -3 / +3
Query: 432 SAPVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR*HEA 271
+APVQY+HSW+FCQ+GCVDNYFTAHYQSPRQMLCLW+KLS GRA CCNYR*H A
Sbjct: 132 AAPVQYIHSWKFCQYGCVDNYFTAHYQSPRQMLCLWDKLSLGRAQCCNYR*HGA 293
Score = 112 bits (239), Expect(2) = 3e-027
Identities = 44/53 (83%), Positives = 45/53 (84%)
Frame = +2 / -3
Query: 272 ASCYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGA 430
A C+L*LQ CA P DS SQRHSICLGDW*CAVK*LSTQP WQN QLW YCTGA
Sbjct: 292 APCHL*LQHCARPNDSLSQRHSICLGDW*CAVK*LSTQPYWQNFQLWMYCTGA 134
Score = 89.5 bits (189), Expect(2) = 7e-028
Identities = 40/51 (78%), Positives = 43/51 (84%)
Frame = -2 / +2
Query: 430 RPRAVCPQLEVLPVRLRGQLLHGALPVPQADAVPLGEAVSGESALL*LQIT 278
RP AV PQLEVLPVRLRGQLLHGALPVPQADAVPLG+ V A+L LQ+T
Sbjct: 134 RPGAVHPQLEVLPVRLRGQLLHGALPVPQADAVPLGQTVVRPRAMLQLQMT 286
Score = 79.4 bits (167), Expect(2) = 3e-048
Identities = 32/39 (82%), Positives = 34/39 (87%)
Frame = -3 / +3
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYNAS 430
VIS IARD+ASRH N SLRLFKMEDVSMGMWVEDYN +
Sbjct: 9 VISIDIARDIASRHANQSLRLFKMEDVSMGMWVEDYNTT 125
Score = 53.8 bits (111), Expect(2) = 7e-028
Identities = 23/38 (60%), Positives = 25/38 (65%)
Frame = -1 / +1
Query: 539 RPALRET*HPGIQTTPYGCSRWRT*AWACGWRTTTPAP 426
R L ET HP Q + GCSRWR *AWACG +TTT P
Sbjct: 16 RLILPETSHPVTQISH*GCSRWRM*AWACGLKTTTLPP 129
Score = 28.6 bits (56), Expect(2) = 3e-027
Identities = 11/21 (52%), Positives = 15/21 (71%)
Frame = +1 / -2
Query: 481 EQP*GVVCMPGCYVSRNAGRD 543
EQP* ++C+ GC VS N R+
Sbjct: 74 EQP**LICVTGCDVSGNINRN 12
>gb|AV915078.1|AV915078 AV915078 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9m07 5', mRNA sequence
Length = 623
Score = 132 bits (282), Expect(2) = 3e-044
Identities = 46/54 (85%), Positives = 51/54 (94%)
Frame = -3 / +3
Query: 432 SAPVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR*HEA 271
+APVQY+HSW+FCQ+GCVDNYFTAHYQSPRQMLCLW+KLS GRA CCNYR*H A
Sbjct: 135 AAPVQYIHSWKFCQYGCVDNYFTAHYQSPRQMLCLWDKLSLGRAQCCNYR*HGA 296
Score = 112 bits (239), Expect(2) = 2e-025
Identities = 44/53 (83%), Positives = 45/53 (84%)
Frame = +2 / -2
Query: 272 ASCYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGA 430
A C+L*LQ CA P DS SQRHSICLGDW*CAVK*LSTQP WQN QLW YCTGA
Sbjct: 295 APCHL*LQHCARPNDSLSQRHSICLGDW*CAVK*LSTQPYWQNFQLWMYCTGA 137
Score = 89.5 bits (189), Expect(2) = 8e-027
Identities = 40/51 (78%), Positives = 43/51 (84%)
Frame = -2 / +2
Query: 430 RPRAVCPQLEVLPVRLRGQLLHGALPVPQADAVPLGEAVSGESALL*LQIT 278
RP AV PQLEVLPVRLRGQLLHGALPVPQADAVPLG+ V A+L LQ+T
Sbjct: 137 RPGAVHPQLEVLPVRLRGQLLHGALPVPQADAVPLGQTVVRPRAMLQLQMT 289
Score = 66.1 bits (138), Expect(2) = 3e-044
Identities = 25/30 (83%), Positives = 27/30 (90%)
Frame = -3 / +3
Query: 519 VASRHTNHSLRLFKMEDVSMGMWVEDYNAS 430
+ASRH N SLRLFKMEDVSMGMWVEDYN +
Sbjct: 39 IASRHANQSLRLFKMEDVSMGMWVEDYNTT 128
Score = 50.1 bits (103), Expect(2) = 8e-027
Identities = 20/34 (58%), Positives = 23/34 (67%)
Frame = -1 / +1
Query: 527 RET*HPGIQTTPYGCSRWRT*AWACGWRTTTPAP 426
+ T HP Q + GCSRWR *AWACG +TTT P
Sbjct: 31 QXTSHPVTQISH*GCSRWRM*AWACGLKTTTLPP 132
Score = 22.6 bits (43), Expect(2) = 2e-025
Identities = 8/14 (57%), Positives = 11/14 (78%)
Frame = +1 / -1
Query: 481 EQP*GVVCMPGCYV 522
EQP* ++C+ GC V
Sbjct: 77 EQP**LICVTGCDV 36
>gb|AV920212.1|AV920212 AV920212 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags9m07 3', mRNA sequence
Length = 671
Score = 131 bits (281), Expect = 3e-030
Identities = 46/53 (86%), Positives = 50/53 (94%)
Frame = -3 / -2
Query: 429 APVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR*HEA 271
APVQY+HSW+FCQ+GCVDNYFTAHYQSPRQMLCLW+KLS GRA CCNYR*H A
Sbjct: 670 APVQYIHSWKFCQYGCVDNYFTAHYQSPRQMLCLWDKLSLGRAQCCNYR*HGA 512
Score = 112 bits (239), Expect = 2e-024
Identities = 44/53 (83%), Positives = 45/53 (84%)
Frame = +2 / +3
Query: 272 ASCYL*LQQCALPADSFSQRHSICLGDW*CAVK*LSTQPNWQNLQLWTYCTGA 430
A C+L*LQ CA P DS SQRHSICLGDW*CAVK*LSTQP WQN QLW YCTGA
Sbjct: 513 APCHL*LQHCARPNDSLSQRHSICLGDW*CAVK*LSTQPYWQNFQLWMYCTGA 671
>gb|AV833885.1|AV833885 AV833885 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags10a02, mRNA sequence
Length = 681
Score = 87.7 bits (185), Expect(2) = 1e-028
Identities = 31/49 (63%), Positives = 35/49 (71%)
Frame = -3 / -3
Query: 426 PVQYVHSWRFCQFGCVDNYFTAHYQSPRQMLCLWEKLSAGRAHCCNYR* 280
PV+Y H RF Q GC D Y TAHYQSP+ M+CLW KL +G HCCN R*
Sbjct: 451 PVEYRHDVRFYQAGCFDGYITAHYQSPQHMICLWRKLQSGSTHCCNVR* 305
Score = 57.9 bits (120), Expect(2) = 1e-028
Identities = 24/37 (64%), Positives = 28/37 (75%)
Frame = -3 / -3
Query: 546 VISAGIARDVASRHTNHSLRLFKMEDVSMGMWVEDYN 436
VIS+ IAR + S +LRLFKMEDVSMGMWVE +N
Sbjct: 574 VISSDIARYIVSEFDTQTLRLFKMEDVSMGMWVEKFN 464
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 310,054,727
Number of Sequences: 312970
Number of extensions: 6098638
Number of successful extensions: 108042
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5640317628
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)