TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QCA13d08.yg.2.1
         (511 letters)

Database: Hordeum_nucl_with_EST.fasta 
           312,970 sequences; 175,134,539 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|BU968786.1|BU968786  HB08J10r BC Hordeum vulgare subsp. v...   155   1e-048

>gb|BU968786.1|BU968786 HB08J10r BC Hordeum vulgare subsp. vulgare cDNA clone HB08J10
           5-PRIME, mRNA sequence
          Length = 581

 Score =  155 bits (334), Expect(2) = 1e-048
 Identities = 64/82 (78%), Positives = 74/82 (90%)
 Frame = +1 / +1

                                                                       
Query: 265 CQGALVGDLSLMCPEFIQSFMAGLAASGVLTSALRLVTKAAFESSKDGLRIGAILFFSIT 444
           C G LVGDLSLMCPEFIQSF++GLAASGV+TSALRL+TKAAFE+S++GLR GA+LFFS+T
Sbjct: 97  C*GGLVGDLSLMCPEFIQSFLSGLAASGVITSALRLITKAAFENSQNGLRNGAMLFFSVT 276

                                 
Query: 445 CLFELVCLLLYTFVFGKLPIVK 510
           C+FEL C LLY  VF KLPIVK
Sbjct: 277 CIFELACFLLYALVFPKLPIVK 342




 Score =  131 bits (281), Expect(2) = 5e-038
 Identities = 58/82 (70%), Positives = 62/82 (75%)
 Frame = -3 / -1

                                                                       
Query: 509 FTMGSLPKTNVYSRRHTSSNRHVIEKNSIAPMRRPSFELSKAALVTNLKADVRTPDAASP 330
           FTMG+L KTN Y R+  SS  HV EKN+IAP RRP  E SKAA V +LKADV TPDAA P
Sbjct: 341 FTMGNLGKTNAYRRKQASSKMHVTEKNNIAPFRRPFCEFSKAAFVISLKADVITPDAAKP 162

                                 
Query: 329 AMKD*MNSGHIKERSPTSAP*Q 264
             KD*MNSGHIKERSPT  P*Q
Sbjct: 161 DRKD*MNSGHIKERSPTKPP*Q 96




 Score =  109 bits (232), Expect(2) = 1e-029
 Identities = 47/82 (57%), Positives = 54/82 (65%)
 Frame = +3 / +3

                                                                       
Query: 264 LSRRTGWRPFFNVPRVHSVLHGGTSCIRGPNISFEISYQGSF*ELKRWSSHWSYTVLFNH 443
           L R  GW PFF+VP VHSVL     CIR  NISFE +Y+GSF*EL +W S WSY +L  +
Sbjct: 96  LLRWLGW*PFFDVPGVHSVLSVRLGCIRSYNISFETNYKGSF*ELTKWPSKWSYVILLGN 275

                                 
Query: 444 MPVRAGVPPAIHIRLRQTTHRE 509
           M  RA + P I I L Q THRE
Sbjct: 276 MHFRASLLPPIRISLSQITHRE 341




 Score =  102 bits (218), Expect(2) = 2e-027
 Identities = 53/89 (59%), Positives = 62/89 (69%)
 Frame = -2 / -3

                                                                       
Query: 510 LHDG*FAEDECV*QEAHQLEQACD*KEQYSSNAKTIF*ALKSCLGN*SQS*C*DP*CS*S 331
           LHDG*F +D CV*+EA  LE AC  +E +SS +K I * LKSCL N*SQS*C + *CS +
Sbjct: 342 LHDG*FGKD*CV*EEAS*LENACYREE*HSSISKAIL*VLKSCLCN*SQS*CYNS*CSQA 163

                                        
Query: 330 RHEGLNELWAH*RKVANQCALTISISCPK 244
             +GLNEL AH RKV NQ  LT +   PK
Sbjct: 162 *QKGLNELRAHQRKVTNQATLTSASDVPK 76




 Score = 83.1 bits (175), Expect(2) = 5e-021
 Identities = 46/82 (56%), Positives = 51/82 (62%)
 Frame = -1 / -2

                                                                       
Query: 511 TSRWVVCRRRMCIAGGTPARTGM*LKRTV*LQCEDHLLSSQKLPW*LISKLMLGPLMQLV 332
           TSRWV+  R M I G   AR  M  +R  *L  E H +SSQKLP *L+SKLML  LMQ  
Sbjct: 343 TSRWVIWERLMRIGGSKLARKCMLPRRIT*LHFEGHFVSSQKLPL*LVSKLML*LLMQPS 164

                                 
Query: 331 PP*RTE*TLGTLKKGRQPVRLD 266
              RTE*T GT KKG QP  L+
Sbjct: 163 LTERTE*TPGTSKKGHQPSHLN 98




 Score = 56.5 bits (117), Expect(2) = 1e-048
 Identities = 20/28 (71%), Positives = 25/28 (89%)
 Frame = +1 / +2

                                       
Query: 178 DVATKGHGGLGVFVGVCIISAIFGTADA 261
           DV TKGHGG+  ++GVCIISA+FGT+DA
Sbjct: 11  DVGTKGHGGIPAYIGVCIISALFGTSDA 94




 Score = 45.5 bits (93), Expect(2) = 5e-038
 Identities = 19/30 (63%), Positives = 20/30 (66%)
 Frame = -3 / -3

                                         
Query: 260 ASAVPNMALIMHTPTKTPSPPCPLVATSRI 171
           AS VP  ALIMHTP     PPCPLV TS +
Sbjct: 93  ASDVPKRALIMHTPMYAGIPPCPLVPTSLV 4




 Score = 40.0 bits (81), Expect(2) = 1e-029
 Identities = 13/23 (56%), Positives = 13/23 (56%)
 Frame = +3 / +1

                                  
Query: 192 RTWWAWCFRWCMHN*RHIWDS*C 260
           RTWW     WCMHN    WD *C
Sbjct: 25  RTWWNSSIHWCMHNQCPFWDI*C 93




 Score = 38.6 bits (78), Expect(2) = 2e-027
 Identities = 15/27 (55%), Positives = 17/27 (62%)
 Frame = -2 / -2

                                      
Query: 258 ISCPKYGANYAYTNENTKPTMSFSCNI 178
           I CPK G +YAYTN     TMSF  N+
Sbjct: 91  IRCPKKGTDYAYTNVCWNSTMSFGTNV 11




 Score = 36.8 bits (74), Expect(2) = 5e-021
 Identities = 16/28 (57%), Positives = 18/28 (64%)
 Frame = -1 / -1

                                       
Query: 259 HQLSQIWR*LCIHQRKHQAHHVL*LQHP 176
           HQ+SQ   *LCIHQ   + HHVL  Q P
Sbjct: 92  HQMSQKGH*LCIHQCMLEFHHVLWYQRP 9



  Database: Hordeum_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:16 PM
  Number of letters in database: 175,134,539
  Number of sequences in database:  312,970
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 256,716,893
Number of Sequences: 312970
Number of extensions: 4044271
Number of successful extensions: 99143
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 50
effective length of database: 42,729,679
effective search space used: 5084831801
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)