TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.068P22F020924.3.1
(714 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG344513.2|BG344513 HVSMEg0009H19f Hordeum vulgare pre-a... 173 3e-067
gb|BG415224.1|BG415224 HVSMEk0005K04f Hordeum vulgare testa... 145 5e-067
gb|AV923358.1|AV923358 AV923358 K. Sato unpublished cDNA li... 251 3e-066
gb|CA009297.1|CA009297 HU13L19r HU Hordeum vulgare subsp. v... 222 2e-057
gb|CA018898.1|CA018898 HV09P17r HV Hordeum vulgare subsp. v... 188 3e-047
gb|BG344237.1|BG344237 HVSMEg0008E18f Hordeum vulgare pre-a... 100 4e-045
gb|BG365417.2|BG365417 HVSMEi0002J17f Hordeum vulgare 20 DA... 109 2e-041
gb|BQ472263.1|BQ472263 HV04O04r HV Hordeum vulgare subsp. v... 100 2e-036
gb|BF631054.2|BF631054 HVSMEb0014N14f Hordeum vulgare seedl... 134 4e-034
gb|AV922764.1|AV922764 AV922764 K. Sato unpublished cDNA li... 107 3e-026
>gb|BG344513.2|BG344513 HVSMEg0009H19f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0009H19f, mRNA sequence
Length = 798
Score = 173 bits (372), Expect(2) = 3e-067
Identities = 71/83 (85%), Positives = 77/83 (92%)
Frame = +2 / +3
Query: 464 GIFISSSVDQSHSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKI 643
GIFISSS DQSHSMSGNGA AFD EYARWLEEHNRQ++ELRA V+AHA DT+LRSVV+KI
Sbjct: 267 GIFISSSADQSHSMSGNGALAFDTEYARWLEEHNRQVNELRAAVNAHAGDTELRSVVEKI 446
Query: 644 MSHYDEIFRLKGNAAKADVFHVL 712
MSHYDEIF+ KGNAAKADVFHVL
Sbjct: 447 MSHYDEIFKQKGNAAKADVFHVL 515
Score = 133 bits (284), Expect(2) = 4e-048
Identities = 58/87 (66%), Positives = 61/87 (70%)
Frame = -2 / -1
Query: 713 IRREKHLPWLHCL*A*KSHHSVT*SYQQHFEDQYHLHEH*HQPLVH*FADCALPTTSHTP 534
I E+HL W H L A*KSHHSVT*S QQH QY LHEH* Q H* DC LPTT TP
Sbjct: 516 ITHERHLLWPHFLFA*KSHHSVT*SSQQHCAAQYRLHEH*LQLSAH*LVDCVLPTTVRTP 337
Query: 533 YQRPMPHFHSWSVTDQLN*I*KCPADE 453
Q+PMPHFHSW+ T LN*I*KCP E
Sbjct: 336 CQKPMPHFHSWNGTGLLN*I*KCPVKE 256
Score = 127 bits (271), Expect(2) = 1e-038
Identities = 58/85 (68%), Positives = 60/85 (70%)
Frame = -1 / -3
Query: 714 DKT*KTSALAALPLSLKISS*CDMILSTTLRRXXXXXXXXXXXXXXLICRLCSSNHLAYS 535
D T*KTSALAA P LKISS*CDMI STTLR L CRLCSSNH AYS
Sbjct: 517 DNT*KTSALAAFPFCLKISS*CDMIFSTTLRSSVSPA*ALTAALSSLTCRLCSSNHRAYS 338
Query: 534 ISKADAPFPLMECD*STELDIKMPC 460
+SKA+APFPLME D S ELDIKMPC
Sbjct: 337 VSKANAPFPLMEWDWSAELDIKMPC 263
Score = 118 bits (252), Expect = 4e-026
Identities = 50/81 (61%), Positives = 63/81 (77%)
Frame = -3 / -2
Query: 706 VKNICLGCIAFEPKNLIIV*HDLINNTSKISITCMSTDTSP*FTNLPIVLFQPPRILHIK 527
+K+IC G I+F KNLIIV*HDL+NNT+++SI CMS + S NL IV FQPP +L +K
Sbjct: 509 MKDICFGRISFLLKNLIIV*HDLLNNTAQLSIACMSINCSSQLINLSIVFFQPPCVLRVK 330
Query: 526 GRCPISTHGV*LIN*TRYKNA 464
+CPISTHG+ L+ *TRYKNA
Sbjct: 329 SQCPISTHGMGLVC*TRYKNA 267
Score = 111 bits (238), Expect(2) = 4e-044
Identities = 53/84 (63%), Positives = 59/84 (70%)
Frame = +3 / +1
Query: 462 RAFLYLVQLISHTP*VEMGHRPLIWSMRGGWKSTIGKLVN*GLVSVLMQVILIFEVLLIR 641
RAFLYLVQ S P*VEMGH L S GGWK+TI KL++* L +LMQ IL VLL R
Sbjct: 265 RAFLYLVQQTSPIP*VEMGHWLLTRSTHGGWKNTIDKLMS*ELQLMLMQAILSCAVLLRR 444
Query: 642 SCHTMMRFLGSKAMQPRQMFFTSY 713
SCHTMMRFL K M+P+QM F Y
Sbjct: 445 SCHTMMRFLSKKEMRPKQMSFMCY 516
Score = 102 bits (217), Expect(2) = 3e-067
Identities = 42/61 (68%), Positives = 51/61 (83%)
Frame = +3 / +2
Query: 282 LTPVTDPKTNMEIKRHCAALLKIVRLQGRAD*GRRHMFNSWRIAG*NLPN*SKSCKELVS 461
LT VTDP+T EIKR C LLKI RLQG+ *G+RHMFN+WRIAG*+LP+*S+SC ELV+
Sbjct: 2 LTLVTDPETETEIKRQCVGLLKIARLQGKVV*GKRHMFNNWRIAG*SLPS*SRSCNELVN 181
Query: 462 R 464
+
Sbjct: 182 K 184
Score = 86.3 bits (182), Expect(3) = 6e-038
Identities = 37/39 (94%), Positives = 39/39 (100%)
Frame = +2 / +1
Query: 311 HGDQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLT 427
+GDQKT+RRLAQNREAARKSRLRKKAYVQQLENSRLKLT
Sbjct: 31 NGDQKTMRRLAQNREAARKSRLRKKAYVQQLENSRLKLT 147
Score = 86.3 bits (182), Expect(2) = 4e-044
Identities = 41/59 (69%), Positives = 45/59 (76%)
Frame = +1 / +3
Query: 283 *LQ*QIQRQTWRSKDIAPPCSKS*GCKEEPTKEEGICSTVGE*QAKTYPTRARAAKSSS 459
*L *QIQRQ RSKD A CSKS GCKE+ +E+GICST+GE*QAK YP RA A SSS
Sbjct: 3 *L**QIQRQKRRSKDNASACSKSRGCKEKSFEEKGICSTIGE*QAKAYPARAGVATSSS 179
Score = 78.5 bits (165), Expect(2) = 4e-048
Identities = 31/44 (70%), Positives = 32/44 (72%)
Frame = -2 / -3
Query: 413 CYSPTVEHMPSSLVGSSLQPHDFEQGGAMSFDLHVCLWICHWSQ 282
CYSP VEHMP S SLQP DFEQ A+SFDL CLWICH SQ
Sbjct: 133 CYSPIVEHMPFSSNDFSLQPRDFEQADALSFDLRFCLWICH*SQ 2
Score = 66.6 bits (139), Expect(3) = 6e-038
Identities = 29/44 (65%), Positives = 32/44 (72%)
Frame = +1 / +2
Query: 454 SSAGHFYI*FS*SVTLHEWKWGIGL*YGVCEVVGRAQSAN**TK 585
S GHFYI*FS V HEWKWGIG *+GV VVGR QS +** +
Sbjct: 257 SFTGHFYI*FSRPVPFHEWKWGIGF*HGVRTVVGRTQSTS**AE 388
Score = 52.8 bits (109), Expect(2) = 1e-038
Identities = 29/49 (59%), Positives = 30/49 (61%)
Frame = -3 / -1
Query: 427 GKF*PAILQLLNIXXXXXXXXXXXLTILSKAAQCLLISMFVFGSVTGVR 281
GK *PAILQLLNI L ILSK CLLIS+ V GSVT VR
Sbjct: 147 GKL*PAILQLLNICLFPQTTFPCSLAILSKPTHCLLISVSVSGSVTRVR 1
Score = 42.8 bits (87), Expect(3) = 6e-038
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = +1 / +2
Query: 640 DHVTL**DF*AQRQCSQGRCFSRLI 714
DHVTL**DF*A+R+C Q RC S +I
Sbjct: 443 DHVTL**DF*AKRKCGQSRCLSCVI 517
>gb|BG415224.1|BG415224 HVSMEk0005K04f Hordeum vulgare testa/pericarp EST library
HVcDNA0013 (normal) Hordeum vulgare subsp. vulgare cDNA
clone HVSMEk0005K04f, mRNA sequence
Length = 898
Score = 145 bits (311), Expect(4) = 5e-067
Identities = 59/71 (83%), Positives = 65/71 (91%)
Frame = +2 / +2
Query: 464 GIFISSSVDQSHSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKI 643
GIFISSS DQSHSMSGNGA AFD EYARWLEEHNRQ++ELRA V+AHA DT+LRSVV+KI
Sbjct: 575 GIFISSSADQSHSMSGNGALAFDTEYARWLEEHNRQVNELRAAVNAHAGDTELRSVVEKI 754
Query: 644 MSHYDEIFRLK 676
MSHYDEIF+ K
Sbjct: 755 MSHYDEIFKQK 787
Score = 117 bits (251), Expect(3) = 4e-045
Identities = 51/73 (69%), Positives = 53/73 (72%)
Frame = -2 / -3
Query: 671 A*KSHHSVT*SYQQHFEDQYHLHEH*HQPLVH*FADCALPTTSHTPYQRPMPHFHSWSVT 492
A*KSHHSVT*S QQH QY LHEH* Q H* DC LPTT TP Q+PMPHFHSW+ T
Sbjct: 782 A*KSHHSVT*SSQQHCAAQYRLHEH*LQLSAH*LVDCVLPTTVRTPCQKPMPHFHSWNGT 603
Query: 491 DQLN*I*KCPADE 453
LN*I*KCP E
Sbjct: 602 GLLN*I*KCPVKE 564
Score = 107 bits (229), Expect(4) = 5e-067
Identities = 44/66 (66%), Positives = 56/66 (84%)
Frame = +3 / +1
Query: 267 VVLMLLTPVTDPKTNMEIKRHCAALLKIVRLQGRAD*GRRHMFNSWRIAG*NLPN*SKSC 446
++L+LLT VTDP+T EIKR C LLKI RLQG+ *G+RHMFN+WRIAG*+LP+*S+SC
Sbjct: 295 LLLLLLTLVTDPETETEIKRQCVGLLKIARLQGKVV*GKRHMFNNWRIAG*SLPS*SRSC 474
Query: 447 KELVSR 464
ELV++
Sbjct: 475 NELVNK 492
Score = 105 bits (224), Expect = 3e-022
Identities = 44/68 (64%), Positives = 54/68 (79%)
Frame = -3 / -1
Query: 667 KNLIIV*HDLINNTSKISITCMSTDTSP*FTNLPIVLFQPPRILHIKGRCPISTHGV*LI 488
KNLIIV*HDL+NNT+++SI CMS + S NL IV FQPP +L +K +CPISTHG+ L+
Sbjct: 778 KNLIIV*HDLLNNTAQLSIACMSINCSSQLINLSIVFFQPPCVLRVKSQCPISTHGMGLV 599
Query: 487 N*TRYKNA 464
*TRYKNA
Sbjct: 598 C*TRYKNA 575
Score = 105 bits (224), Expect(3) = 3e-034
Identities = 48/81 (59%), Positives = 51/81 (62%)
Frame = -1 / -2
Query: 702 KTSALAALPLSLKISS*CDMILSTTLRRXXXXXXXXXXXXXXLICRLCSSNHLAYSISKA 523
K + LKISS*CDMI STTLR L CRLCSSNH AYS+SKA
Sbjct: 813 KDICFXRISFCLKISS*CDMIFSTTLRSSVSPA*ALTAALSSLTCRLCSSNHRAYSVSKA 634
Query: 522 DAPFPLMECD*STELDIKMPC 460
+APFPLME D S ELDIKMPC
Sbjct: 633 NAPFPLMEWDWSAELDIKMPC 571
Score = 96.8 bits (205), Expect(2) = 1e-041
Identities = 47/72 (65%), Positives = 51/72 (70%)
Frame = +3 / +3
Query: 462 RAFLYLVQLISHTP*VEMGHRPLIWSMRGGWKSTIGKLVN*GLVSVLMQVILIFEVLLIR 641
RAFLYLVQ S P*VEMGH L S GGWK+TI KL++* L +LMQ IL VLL R
Sbjct: 573 RAFLYLVQQTSPIP*VEMGHWLLTRSTHGGWKNTIDKLMS*ELQLMLMQAILSCAVLLRR 752
Query: 642 SCHTMMRFLGSK 677
SCHTMMRFL K
Sbjct: 753 SCHTMMRFLSKK 788
Score = 93.2 bits (197), Expect(2) = 1e-041
Identities = 43/61 (70%), Positives = 47/61 (77%)
Frame = +1 / +2
Query: 277 CF*LQ*QIQRQTWRSKDIAPPCSKS*GCKEEPTKEEGICSTVGE*QAKTYPTRARAAKSS 456
CF*L *QIQRQ RSKD A CSKS GCKE+ +E+GICST+GE*QAK YP RA A SS
Sbjct: 305 CF*L**QIQRQKRRSKDNASACSKSRGCKEKSFEEKGICSTIGE*QAKAYPARAGVATSS 484
Query: 457 S 459
S
Sbjct: 485 S 487
Score = 86.3 bits (182), Expect(3) = 9e-034
Identities = 37/39 (94%), Positives = 39/39 (100%)
Frame = +2 / +3
Query: 311 HGDQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLT 427
+GDQKT+RRLAQNREAARKSRLRKKAYVQQLENSRLKLT
Sbjct: 339 NGDQKTMRRLAQNREAARKSRLRKKAYVQQLENSRLKLT 455
Score = 83.1 bits (175), Expect(3) = 4e-045
Identities = 33/47 (70%), Positives = 34/47 (72%)
Frame = -2 / -2
Query: 413 CYSPTVEHMPSSLVGSSLQPHDFEQGGAMSFDLHVCLWICHWSQKHQ 273
CYSP VEHMP S SLQP DFEQ A+SFDL CLWICH SQK Q
Sbjct: 441 CYSPIVEHMPFSSNDFSLQPRDFEQADALSFDLRFCLWICH*SQKQQ 301
Score = 66.6 bits (139), Expect(3) = 9e-034
Identities = 29/44 (65%), Positives = 32/44 (72%)
Frame = +1 / +1
Query: 454 SSAGHFYI*FS*SVTLHEWKWGIGL*YGVCEVVGRAQSAN**TK 585
S GHFYI*FS V HEWKWGIG *+GV VVGR QS +** +
Sbjct: 565 SFTGHFYI*FSRPVPFHEWKWGIGF*HGVRTVVGRTQSTS**AE 696
Score = 55.1 bits (114), Expect(3) = 3e-034
Identities = 31/55 (56%), Positives = 33/55 (60%)
Frame = -3 / -3
Query: 427 GKF*PAILQLLNIXXXXXXXXXXXLTILSKAAQCLLISMFVFGSVTGVRSIKTTS 263
GK *PAILQLLNI L ILSK CLLIS+ V GSVT VRS +S
Sbjct: 455 GKL*PAILQLLNICLFPQTTFPCSLAILSKPTHCLLISVSVSGSVTRVRSNSKSS 291
Score = 30.4 bits (60), Expect(4) = 5e-067
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +3 / +1
Query: 186 LQWQMPALEQKHQQMIL 236
LQWQ P LEQK Q +++
Sbjct: 214 LQWQRPVLEQKRQMILM 264
Score = 28.6 bits (56), Expect(3) = 9e-034
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +1 / +1
Query: 640 DHVTL**DF*AQRQCSQGRCF 702
DHVTL**DF*A+R + F
Sbjct: 751 DHVTL**DF*AKRNAXKADVF 813
Score = 28.1 bits (55), Expect(4) = 5e-067
Identities = 12/16 (75%), Positives = 12/16 (75%)
Frame = +2 / +1
Query: 665 FRLKGNAAKADVFHVL 712
F K NA KADVFHVL
Sbjct: 775 F*AKRNAXKADVFHVL 822
Score = 22.6 bits (43), Expect(3) = 3e-034
Identities = 6/9 (66%), Positives = 7/9 (77%)
Frame = -3 / -3
Query: 208 RAGICHCNL 182
R G+CHC L
Sbjct: 236 RTGLCHCRL 210
Score = 22.6 bits (43), Expect(3) = 4e-045
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = -2 / -2
Query: 224 LMFLF*GWHLPL 189
L FLF* W LPL
Sbjct: 252 LTFLF*DWPLPL 217
>gb|AV923358.1|AV923358 AV923358 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd12m24 5', mRNA sequence
Length = 556
Score = 251 bits (543), Expect = 3e-066
Identities = 108/134 (80%), Positives = 116/134 (86%)
Frame = +2 / +2
Query: 311 HGDQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVD 490
+GDQKT+RRLAQNREAARKSRLRKKAYVQQLENSRLKLT GIFISSS D
Sbjct: 107 NGDQKTMRRLAQNREAARKSRLRKKAYVQQLENSRLKLTQLEQELQRARQQGIFISSSAD 286
Query: 491 QSHSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFR 670
QSHSMSGNGA AFD EYARWLEEHNRQ++ELRA V+AHA DT+LRSVV+KIMSHYDEIF+
Sbjct: 287 QSHSMSGNGALAFDTEYARWLEEHNRQVNELRAAVNAHAGDTELRSVVEKIMSHYDEIFK 466
Query: 671 LKGNAAKADVFHVL 712
KGNAAKADVFHVL
Sbjct: 467 QKGNAAKADVFHVL 508
Score = 214 bits (462), Expect = 4e-055
Identities = 96/149 (64%), Positives = 114/149 (76%)
Frame = +3 / +3
Query: 267 VVLMLLTPVTDPKTNMEIKRHCAALLKIVRLQGRAD*GRRHMFNSWRIAG*NLPN*SKSC 446
++L+LLT VTDP+T EIKR C LLKI RLQG+ *G+RHMFN+WRIAG*+LP+*S+SC
Sbjct: 63 LLLLLLTLVTDPETETEIKRQCVGLLKIARLQGKVV*GKRHMFNNWRIAG*SLPS*SRSC 242
Query: 447 KELVSRAFLYLVQLISHTP*VEMGHRPLIWSMRGGWKSTIGKLVN*GLVSVLMQVILIFE 626
ELV++AFLYLVQ S P*VEMGH L S GGWK+TI KL++* L +LMQ IL
Sbjct: 243 NELVNKAFLYLVQQTSPIP*VEMGHWLLTRSTHGGWKNTIDKLMS*ELQLMLMQAILSCA 422
Query: 627 VLLIRSCHTMMRFLGSKAMQPRQMFFTSY 713
VLL RSCHTMMRFL K M+P+QM F Y
Sbjct: 423 VLLRRSCHTMMRFLSKKEMRPKQMSFMCY 509
Score = 206 bits (445), Expect = 9e-053
Identities = 92/147 (62%), Positives = 96/147 (65%)
Frame = -2 / -3
Query: 713 IRREKHLPWLHCL*A*KSHHSVT*SYQQHFEDQYHLHEH*HQPLVH*FADCALPTTSHTP 534
I E+HL W H L A*KSHHSVT*S QQH QY LHEH* Q H* DC LPTT TP
Sbjct: 509 ITHERHLLWPHFLFA*KSHHSVT*SSQQHCAAQYRLHEH*LQLSAH*LVDCVLPTTVRTP 330
Query: 533 YQRPMPHFHSWSVTDQLN*I*KCPADEXXXXXXXXXXXXACYSPTVEHMPSSLVGSSLQP 354
Q+PMPHFHSW+ T LN*I*KC DE ACYSP VEHMP S SLQP
Sbjct: 329 CQKPMPHFHSWNGTGLLN*I*KCLVDELVATPALAG*ALACYSPIVEHMPFSSNDFSLQP 150
Query: 353 HDFEQGGAMSFDLHVCLWICHWSQKHQ 273
DFEQ A+SFDL CLWICH SQK Q
Sbjct: 149 RDFEQADALSFDLRFCLWICH*SQKQQ 69
Score = 165 bits (354), Expect = 3e-040
Identities = 82/148 (55%), Positives = 97/148 (65%)
Frame = -3 / -1
Query: 706 VKNICLGCIAFEPKNLIIV*HDLINNTSKISITCMSTDTSP*FTNLPIVLFQPPRILHIK 527
+K+IC G I+F KNLIIV*HDL+NNT+++SI CMS + S NL IV FQPP +L +K
Sbjct: 502 MKDICFGRISFLLKNLIIV*HDLLNNTAQLSIACMSINCSSQLINLSIVFFQPPCVLRVK 323
Query: 526 GRCPISTHGV*LIN*TRYKNAXXXXXXXXXXXLGKF*PAILQLLNIXXXXXXXXXXXLTI 347
+CPISTHG+ L+ *TRYKNA LGK *PAILQLLNI L I
Sbjct: 322 SQCPISTHGMGLVC*TRYKNALLTSSLQLLL*LGKL*PAILQLLNICLFPQTTFPCSLAI 143
Query: 346 LSKAAQCLLISMFVFGSVTGVRSIKTTS 263
LSK CLLIS+ V GSVT VRS +S
Sbjct: 142 LSKPTHCLLISVSVSGSVTRVRSNSKSS 59
Score = 152 bits (326), Expect(2) = 4e-043
Identities = 70/103 (67%), Positives = 77/103 (74%)
Frame = +1 / +1
Query: 277 CF*LQ*QIQRQTWRSKDIAPPCSKS*GCKEEPTKEEGICSTVGE*QAKTYPTRARAAKSS 456
CF*L *QIQRQ RSKD A CSKS GCKE+ +E+GICST+GE*QAK YP RA A SS
Sbjct: 73 CF*L**QIQRQKRRSKDNASACSKSRGCKEKSFEEKGICSTIGE*QAKAYPARAGVATSS 252
Query: 457 SAGHFYI*FS*SVTLHEWKWGIGL*YGVCEVVGRAQSAN**TK 585
S HFYI*FS V HEWKWGIG *+GV VVGR QS +** +
Sbjct: 253 STRHFYI*FSRPVPFHEWKWGIGF*HGVRTVVGRTQSTS**AE 381
Score = 138 bits (296), Expect = 3e-032
Identities = 67/109 (61%), Positives = 69/109 (63%)
Frame = -1 / -2
Query: 714 DKT*KTSALAALPLSLKISS*CDMILSTTLRRXXXXXXXXXXXXXXLICRLCSSNHLAYS 535
D T*KTSALAA P LKISS*CDMI STTLR L CRLCSSNH AYS
Sbjct: 510 DNT*KTSALAAFPFCLKISS*CDMIFSTTLRSSVSPA*ALTAALSSLTCRLCSSNHRAYS 331
Query: 534 ISKADAPFPLMECD*STELDIKMPC*RAXXXXXXXXXXXXXXXSNC*TY 388
+SKA+APFPLME D S ELDIKMPC*RA SNC*TY
Sbjct: 330 VSKANAPFPLMEWDWSAELDIKMPC*RARCNSCSSWVSFSLLFSNC*TY 184
Score = 42.8 bits (87), Expect(2) = 4e-043
Identities = 18/25 (72%), Positives = 21/25 (84%)
Frame = +1 / +1
Query: 640 DHVTL**DF*AQRQCSQGRCFSRLI 714
DHVTL**DF*A+R+C Q RC S +I
Sbjct: 436 DHVTL**DF*AKRKCGQSRCLSCVI 510
>gb|CA009297.1|CA009297 HU13L19r HU Hordeum vulgare subsp. vulgare cDNA clone HU13L19
5-PRIME, mRNA sequence
Length = 625
Score = 222 bits (479), Expect = 2e-057
Identities = 94/132 (71%), Positives = 109/132 (82%)
Frame = +2 / +3
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQS 496
DQKTLRRLAQNREAARKSRLRKK+YVQQLE+S+LKL GIFISSS DQ+
Sbjct: 33 DQKTLRRLAQNREAARKSRLRKKSYVQQLESSKLKLAQLEQELQKARQQGIFISSSGDQT 212
Query: 497 HSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLK 676
H+MSGNGA FD+EY RWLE+ N+QI+ELR V+AHASD+DLR +VD IMSHYDEIF++K
Sbjct: 213 HAMSGNGAMTFDLEYTRWLEDQNKQINELRTAVNAHASDSDLRLIVDGIMSHYDEIFKVK 392
Query: 677 GNAAKADVFHVL 712
G AAKADVFH+L
Sbjct: 393 GVAAKADVFHIL 428
>gb|CA018898.1|CA018898 HV09P17r HV Hordeum vulgare subsp. vulgare cDNA clone HV09P17
5-PRIME, mRNA sequence
Length = 414
Score = 188 bits (405), Expect = 3e-047
Identities = 80/114 (70%), Positives = 92/114 (80%)
Frame = +2 / +1
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQS 496
DQKTLRRLAQNREAARKSRLRKK+YVQQLE+S+LKL GIFISSS DQ+
Sbjct: 73 DQKTLRRLAQNREAARKSRLRKKSYVQQLESSKLKLAQLEQELQKARQQGIFISSSGDQT 252
Query: 497 HSMSGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYD 658
H+MSGNGA FD+EY RWLE+ N+QI+ELR V+AHASD+DLR +VD IMSHYD
Sbjct: 253 HAMSGNGAMTFDLEYTRWLEDQNKQINELRTAVNAHASDSDLRLIVDGIMSHYD 414
>gb|BG344237.1|BG344237 HVSMEg0008E18f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0008E18f, mRNA sequence
Length = 766
Score = 100 bits (213), Expect(5) = 4e-045
Identities = 42/68 (61%), Positives = 54/68 (79%)
Frame = +3 / +3
Query: 267 VVLMLLTPVTDPKTNMEIKRHCAALLKIVRLQGRAD*GRRHMFNSWRIAG*NLPN*SKSC 446
++L+LLT VTDP+T EIKR C LLKI RLQG+ * +RHMFN+W IAG*+LP+*S+ C
Sbjct: 372 LLLLLLTLVTDPETETEIKRQCVGLLKIARLQGKVV*RKRHMFNNWGIAG*SLPS*SRRC 551
Query: 447 KELVSRAF 470
ELV++ F
Sbjct: 552 NELVNKHF 575
Score = 94.5 bits (200), Expect(2) = 1e-023
Identities = 44/65 (67%), Positives = 48/65 (73%)
Frame = +1 / +1
Query: 277 CF*LQ*QIQRQTWRSKDIAPPCSKS*GCKEEPTKEEGICSTVGE*QAKTYPTRARAAKSS 456
CF*L *QIQRQ RSKD A CSKS GCKE+ +E+GICST+GE*QAK YP RA A SS
Sbjct: 382 CF*L**QIQRQKRRSKDNASACSKSRGCKEKSFEEKGICSTIGE*QAKAYPARAGGATSS 561
Query: 457 SAGHF 471
S F
Sbjct: 562 STSIF 576
Score = 83.1 bits (175), Expect(3) = 2e-026
Identities = 33/47 (70%), Positives = 34/47 (72%)
Frame = -2 / -3
Query: 413 CYSPTVEHMPSSLVGSSLQPHDFEQGGAMSFDLHVCLWICHWSQKHQ 273
CYSP VEHMP S SLQP DFEQ A+SFDL CLWICH SQK Q
Sbjct: 518 CYSPIVEHMPFSSNDFSLQPRDFEQADALSFDLRFCLWICH*SQKQQ 378
Score = 81.7 bits (172), Expect(2) = 2e-024
Identities = 35/39 (89%), Positives = 38/39 (97%)
Frame = +2 / +2
Query: 311 HGDQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLT 427
+GDQKT+RRLAQNREAARKSRL+KKAYVQQL NSRLKLT
Sbjct: 416 NGDQKTMRRLAQNREAARKSRLKKKAYVQQLGNSRLKLT 532
Score = 57.0 bits (118), Expect(5) = 4e-045
Identities = 24/27 (88%), Positives = 24/27 (88%)
Frame = +2 / +1
Query: 467 IFISSSVDQSHSMSGNGASAFDMEYAR 547
IFISSS DQSHSMSGNGA AFD EYAR
Sbjct: 571 IFISSSADQSHSMSGNGALAFDTEYAR 651
Score = 50.6 bits (104), Expect(2) = 2e-024
Identities = 21/38 (55%), Positives = 24/38 (63%)
Frame = +1 / +3
Query: 427 PTRARAAKSSSAGHFYI*FS*SVTLHEWKWGIGL*YGV 540
P+ +R HFYI*FS V HEWKWGIG *+GV
Sbjct: 531 PS*SRRCNELVNKHFYI*FSRPVPFHEWKWGIGF*HGV 644
Score = 50.6 bits (104), Expect(3) = 2e-026
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = -2 / -1
Query: 551 TTSHTPYQRPMPHFHSWSVTDQLN*I*KC 465
T TP Q+PMPHFHSW+ T LN*I*KC
Sbjct: 655 THVRTPCQKPMPHFHSWNGTGLLN*I*KC 569
Score = 42.8 bits (87), Expect(5) = 4e-045
Identities = 16/26 (61%), Positives = 20/26 (76%)
Frame = +2 / +3
Query: 548 WLEEHNRQISELRAGVSAHASDTDLR 625
W+EEHNR I EL+A V+A DT+LR
Sbjct: 651 WVEEHNRPIYELKAAVNASGGDTELR 728
Score = 35.0 bits (70), Expect(2) = 1e-023
Identities = 18/33 (54%), Positives = 21/33 (63%)
Frame = +3 / +2
Query: 462 RAFLYLVQLISHTP*VEMGHRPLIWSMRGGWKS 560
+AFLYLVQ S P*VEMGH L S G ++
Sbjct: 566 QAFLYLVQQTSPIP*VEMGHWLLTRSTHVGGRT 664
Score = 30.4 bits (60), Expect(5) = 4e-045
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +3 / +3
Query: 186 LQWQMPALEQKHQQMIL 236
LQWQ P LEQK Q +++
Sbjct: 291 LQWQRPVLEQKRQMILM 341
Score = 24.4 bits (47), Expect(5) = 4e-045
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = +2 / +1
Query: 629 VVDKIMSHYDE 661
VV KIMSH+DE
Sbjct: 733 VVGKIMSHFDE 765
Score = 22.6 bits (43), Expect(3) = 2e-026
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = -2 / -3
Query: 224 LMFLF*GWHLPL 189
L FLF* W LPL
Sbjct: 329 LTFLF*DWPLPL 294
>gb|BG365417.2|BG365417 HVSMEi0002J17f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0002J17f, mRNA sequence
Length = 844
Score = 109 bits (233), Expect(2) = 2e-041
Identities = 49/74 (66%), Positives = 55/74 (74%)
Frame = +2 / +2
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVDQS 496
DQK LRRLAQNREAARKSRLRKKAYVQQLE+S+LKL GIFI SS DQ+
Sbjct: 428 DQKVLRRLAQNREAARKSRLRKKAYVQQLESSKLKLASLEQELQKARQQGIFIFSSGDQT 607
Query: 497 HSMSGNGASAFDME 538
H+MSGNGA D++
Sbjct: 608 HAMSGNGAMTLDLD 649
Score = 79.4 bits (167), Expect(2) = 2e-041
Identities = 31/47 (65%), Positives = 37/47 (78%)
Frame = +2 / +1
Query: 539 YARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKG 679
Y RWLEE N+QI ELR +AHASD+D +VD IM+HYDEIF+LKG
Sbjct: 649 YTRWLEEQNKQIKELRTASNAHASDSDPPLIVDGIMAHYDEIFKLKG 789
>gb|BQ472263.1|BQ472263 HV04O04r HV Hordeum vulgare subsp. vulgare cDNA clone HV04O04
5-PRIME, mRNA sequence
Length = 662
Score = 100 bits (214), Expect(2) = 2e-036
Identities = 37/69 (53%), Positives = 54/69 (78%)
Frame = +2 / +2
Query: 506 SGNGASAFDMEYARWLEEHNRQISELRAGVSAHASDTDLRSVVDKIMSHYDEIFRLKGNA 685
S GA+ FD+EY RWL++ +R++ ELR G+ AH D DLR+++D ++HYDE+FRLK A
Sbjct: 386 SSPGAAMFDVEYNRWLDDDSRRMIELRGGLHAHLPDGDLRAIIDDTLTHYDELFRLKSAA 565
Query: 686 AKADVFHVL 712
A+ADVFH++
Sbjct: 566 ARADVFHLI 592
Score = 71.6 bits (150), Expect(2) = 2e-036
Identities = 31/56 (55%), Positives = 37/56 (66%)
Frame = +2 / +2
Query: 317 DQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSS 484
D KT RRLAQNREAARKSRLRKKAY+QQLE+ +LKL G+ + +
Sbjct: 206 DPKTTRRLAQNREAARKSRLRKKAYIQQLESGKLKLAQLEQDLQRARSQGLLVGGA 373
>gb|BF631054.2|BF631054 HVSMEb0014N14f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0014N14f, mRNA sequence
Length = 768
Score = 134 bits (287), Expect(2) = 4e-034
Identities = 61/83 (73%), Positives = 65/83 (78%)
Frame = +2 / +3
Query: 311 HGDQKTLRRLAQNREAARKSRLRKKAYVQQLENSRLKLTXXXXXXXXXXXXGIFISSSVD 490
+GDQKT+RRLAQNREAARKSRLRKKAYVQQLENSRLKLT GIFISSS D
Sbjct: 453 NGDQKTMRRLAQNREAARKSRLRKKAYVQQLENSRLKLTQLEQELQRARQQGIFISSSAD 632
Query: 491 QSHSMSGNGASAFDMEYARWLEE 559
QSHSM NGA AFD EYAR +E+
Sbjct: 633 QSHSMEWNGALAFDTEYARVVEK 701
Score = 127 bits (272), Expect = 6e-029
Identities = 59/97 (60%), Positives = 71/97 (73%)
Frame = +3 / +1
Query: 267 VVLMLLTPVTDPKTNMEIKRHCAALLKIVRLQGRAD*GRRHMFNSWRIAG*NLPN*SKSC 446
++L+LLT VTDP+T EIKR C LLKI RLQG+ *G+RHMFN+WRIAG*+LP+*S+SC
Sbjct: 409 LLLLLLTLVTDPETETEIKRQCVGLLKIARLQGKVV*GKRHMFNNWRIAG*SLPS*SRSC 588
Query: 447 KELVSRAFLYLVQLISHTP*VEMGHRPLIWSMRGGWK 557
ELV++AFLYLVQ S P MG L S G K
Sbjct: 589 NELVNKAFLYLVQQTSPIPWSGMGLWLLTRSTHGWLK 699
Score = 126 bits (269), Expect = 2e-028
Identities = 58/88 (65%), Positives = 64/88 (72%)
Frame = +1 / +2
Query: 277 CF*LQ*QIQRQTWRSKDIAPPCSKS*GCKEEPTKEEGICSTVGE*QAKTYPTRARAAKSS 456
CF*L *QIQRQ RSKD A CSKS GCKE+ +E+GICST+GE*QAK YP RA A SS
Sbjct: 419 CF*L**QIQRQKRRSKDNASACSKSRGCKEKSFEEKGICSTIGE*QAKAYPARAGVATSS 598
Query: 457 SAGHFYI*FS*SVTLHEWKWGIGL*YGV 540
S HFYI*FS V H +WG G *+GV
Sbjct: 599 STRHFYI*FSRPVPFHGVEWGFGF*HGV 682
Score = 115 bits (246), Expect(3) = 5e-030
Identities = 51/89 (57%), Positives = 54/89 (60%)
Frame = -2 / -1
Query: 539 TPYQRPMPHFHSWSVTDQLN*I*KCPADEXXXXXXXXXXXXACYSPTVEHMPSSLVGSSL 360
TP Q+P PH W+ T LN*I*KC DE ACYSP VEHMP S SL
Sbjct: 681 TPCQKPKPHSTPWNGTGLLN*I*KCLVDELVATPALAG*ALACYSPIVEHMPFSSNDFSL 502
Query: 359 QPHDFEQGGAMSFDLHVCLWICHWSQKHQ 273
QP DFEQ A+SFDL CLWICH SQK Q
Sbjct: 501 QPRDFEQADALSFDLRFCLWICH*SQKQQ 415
Score = 30.4 bits (60), Expect(2) = 4e-034
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +3 / +1
Query: 186 LQWQMPALEQKHQQMIL 236
LQWQ P LEQK Q +++
Sbjct: 328 LQWQRPVLEQKRQMILM 378
Score = 30.4 bits (60), Expect(3) = 5e-030
Identities = 13/24 (54%), Positives = 14/24 (58%)
Frame = -3 / -1
Query: 616 SITCMSTDTSP*FTNLPIVLFQPP 545
S+ CMS S NL IV FQPP
Sbjct: 759 SMACMSIKCSSQVNNLSIVFFQPP 688
Score = 22.6 bits (43), Expect(3) = 5e-030
Identities = 9/12 (75%), Positives = 9/12 (75%)
Frame = -2 / -1
Query: 224 LMFLF*GWHLPL 189
L FLF* W LPL
Sbjct: 366 LTFLF*DWPLPL 331
>gb|AV922764.1|AV922764 AV922764 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd2e15 5', mRNA sequence
Length = 652
Score = 107 bits (229), Expect(2) = 3e-026
Identities = 44/66 (66%), Positives = 56/66 (84%)
Frame = +3 / +2
Query: 267 VVLMLLTPVTDPKTNMEIKRHCAALLKIVRLQGRAD*GRRHMFNSWRIAG*NLPN*SKSC 446
++L+LLT VTDP+T EIKR C LLKI RLQG+ *G+RHMFN+WRIAG*+LP+*S+SC
Sbjct: 371 LLLLLLTLVTDPETETEIKRQCVGLLKIARLQGKVV*GKRHMFNNWRIAG*SLPS*SRSC 550
Query: 447 KELVSR 464
ELV++
Sbjct: 551 NELVNK 568
Score = 30.4 bits (60), Expect(2) = 3e-026
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +3 / +2
Query: 186 LQWQMPALEQKHQQMIL 236
LQWQ P LEQK Q +++
Sbjct: 290 LQWQRPVLEQKRQMILM 340
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 291,772,570
Number of Sequences: 312970
Number of extensions: 4016825
Number of successful extensions: 36352
Number of sequences better than 1.0e-020: 14
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7889507874
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)