TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBTB.063O07F020916.3.1
(657 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF623444.2|BF623444 HVSMEa0002B20f Hordeum vulgare seedl... 213 7e-055
gb|CA029310.1|CA029310 HZ64N08r HZ Hordeum vulgare subsp. v... 132 2e-034
>gb|BF623444.2|BF623444 HVSMEa0002B20f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0002B20f, mRNA sequence
Length = 846
Score = 213 bits (460), Expect = 7e-055
Identities = 90/117 (76%), Positives = 104/117 (88%)
Frame = +2 / +1
Query: 284 SEKYMG*SWHFMGGSEDDEPPSKRARTSSVESASLPDCFSFSKFSNPLGSTMARPLPSQG 463
S++YMG* WHFMGG EDDEPPSKRAR SSVESASL D +SK +NPLG TMARPL SQG
Sbjct: 91 SKEYMG*FWHFMGGFEDDEPPSKRARASSVESASLSDIPCYSKPANPLGGTMARPLTSQG 270
Query: 464 KEVMVGSKGVIKKEEFVRIITKTLYSLGYEKSGAVLEEESGIILHNPMVKLFREQVI 634
KEVMVGSKG+IK++EFVRI+TK+LY+LGYEK+GAVLEEESGI LH+P V LFR+QV+
Sbjct: 271 KEVMVGSKGLIKRDEFVRILTKSLYTLGYEKTGAVLEEESGITLHSPPVNLFRKQVL 441
Score = 202 bits (436), Expect = 1e-051
Identities = 87/117 (74%), Positives = 94/117 (80%)
Frame = -1 / -2
Query: 642 FHPITCSLKSFTIGLCKIIPDSSSRTAPLFSYPRLYRVLVIILTNSSFLITPLEPTITSF 463
F TC LK FT G C +IPDSSSRTAP+FSYPR+YR V ILTNSS LI PLEPTITSF
Sbjct: 449 FPSSTCFLKRFTGGECSVIPDSSSRTAPVFSYPRVYRDFVSILTNSSLLIKPLEPTITSF 270
Query: 462 PWEGKGLAIVLPKGLENFENEKQSGKLADSTEDVLARFEGGSSSSEPPMKCQDQPIY 292
PWE KGLAIV PKGL E + S KLADSTED LARF+GGSSSS+PPMKCQ+QPIY
Sbjct: 269 PWEVKGLAIVPPKGLAGLE*QGMSDKLADSTEDALARFDGGSSSSKPPMKCQNQPIY 99
Score = 143 bits (306), Expect = 1e-033
Identities = 68/101 (67%), Positives = 75/101 (74%)
Frame = -2 / -3
Query: 584 LIPPPELLHFFHIQDYTEFW*LS*QIPPS**HPWNQP*LLSLGKAKV*PLYFPKDWKISK 405
LIPP E L FFHIQ+YTE *+S QI PS* + NQP*LLSLGK+KV*PLY PKD ++
Sbjct: 391 LIPPQEQLQFFHIQEYTEIL*VSSQIHPS*LNL*NQP*LLSLGKSKV*PLYLPKD*RV*N 212
Query: 404 TRNSLVNLQILLRMFLRVLRVARHLQNLP*NAKISPYIFHF 282
R L NL ILLRM L VL HLQNLP*NAKISPYI +F
Sbjct: 211 NRECLTNLLILLRMLLPVLMAVHHLQNLP*NAKISPYILYF 89
Score = 120 bits (257), Expect(2) = 6e-033
Identities = 58/85 (68%), Positives = 64/85 (75%)
Frame = +1 / +3
Query: 283 K*KIYGLILAFHGRF*R*RATLKTRKNILSRICKFTRLFLVFEIFQSFGKYNG*TFAFPR 462
K*+IYGLILAFHGRF*R* +KT K+ILSRI KF R L+F SFG+YNG*TF FPR
Sbjct: 90 K*RIYGLILAFHGRF*R**TAIKTGKSILSRISKFVRHSLLF*TR*SFGRYNG*TFDFPR 269
Query: 463 ERSYGWFQGCY*EGGIC*DNHQNSV 537
ERSYGWF *EG IC D H+ SV
Sbjct: 270 ERSYGWF*RFN*EG*ICEDTHKISV 344
Score = 112 bits (239), Expect(2) = 8e-025
Identities = 51/86 (59%), Positives = 58/86 (67%)
Frame = +3 / +2
Query: 213 ESSICFGNCLQQFVWRLHTITFPEVKNIWADLGISWEVLKMTSHPQNAQEHPQ*NLQVYQ 392
E+++ + QF+ T+ EVKNIWAD GISWEVLKM + QN QEHPQ*N QV Q
Sbjct: 20 ETAVTYRFLWWQFLVASVTLFSTEVKNIWADFGISWEVLKMMNRHQNGQEHPQ*NQQVCQ 199
Query: 393 TVSRFRNFPILWEVQWLDLCLPKGKK 470
T N ILWEVQWLDL LPKGKK
Sbjct: 200 TFPVILNPLILWEVQWLDL*LPKGKK 277
Score = 105 bits (225), Expect(2) = 2e-031
Identities = 47/73 (64%), Positives = 51/73 (69%)
Frame = -3 / -1
Query: 463 PLGRQRSSHCTSQRIGKFRKRETVW*TCRFY*GCSCAF*GWLVIFRTSHEMPRSAHIFFT 284
PLG QRSSHCTSQRI FR V TC FY*GCSC F* +IF+TSHEMP+SAHIFFT
Sbjct: 270 PLGSQRSSHCTSQRISGFRITGNV*QTC*FY*GCSCPF*WRFIIFKTSHEMPKSAHIFFT 91
Query: 283 SGKVMVCNLHTNC 245
S + V NC
Sbjct: 90 SVENNVTEATRNC 52
Score = 49.2 bits (101), Expect(2) = 2e-031
Identities = 23/44 (52%), Positives = 30/44 (68%)
Frame = -3 / -1
Query: 640 PSNHLFSEKFHHRVM*NNP*FLLQNCSTFFISKTIQSFGDYPNK 509
P +L S+K H M *FLL+N S+FFISK+IQ F +YP+K
Sbjct: 447 PIKYLLSKKIHWWRMQCYT*FLLKNSSSFFISKSIQRFCEYPHK 316
Score = 40.0 bits (81), Expect(2) = 6e-033
Identities = 23/42 (54%), Positives = 26/42 (61%)
Frame = +3 / +2
Query: 516 G*SPKLCIVLDMKKVEQFWRRNQGLFYITLW*NFSENR*LDG 641
G S LCI+LDMKK+E F RRNQ I * F E+R L G
Sbjct: 323 GYSQNLCILLDMKKLELFLRRNQV*HCILHQ*IFLESRYLMG 448
Score = 23.1 bits (44), Expect(2) = 8e-025
Identities = 8/15 (53%), Positives = 9/15 (60%)
Frame = +3 / +1
Query: 612 NFSENR*LDGNWDNA 656
N + LDGNWD A
Sbjct: 418 NLFRKQVLDGNWDGA 462
>gb|CA029310.1|CA029310 HZ64N08r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ64N08
5-PRIME, mRNA sequence
Length = 367
Score = 132 bits (283), Expect(3) = 2e-034
Identities = 54/67 (80%), Positives = 58/67 (86%)
Frame = -1 / -2
Query: 489 PLEPTITSFPWEGKGLAIVLPKGLENFENEKQSGKLADSTEDVLARFEGGSSSSEPPMKC 310
PLEPTITSFPWE KGLAIV PKGL +FE +K S K+ DSTED LARFEGGSSSS+PPMKC
Sbjct: 366 PLEPTITSFPWEAKGLAIVPPKGLMDFE*QKLSDKIVDSTEDALARFEGGSSSSKPPMKC 187
Query: 309 QDQPIYF 289
Q QPIYF
Sbjct: 186 QSQPIYF 166
Score = 127 bits (271), Expect(3) = 3e-031
Identities = 55/73 (75%), Positives = 59/73 (80%)
Frame = +2 / +2
Query: 272 HLP*SEKYMG*SWHFMGGSEDDEPPSKRARTSSVESASLPDCFSFSKFSNPLGSTMARPL 451
+L * +KYMG* WHFMGG EDDEPPSKRAR SSVES L + F +SK NPLG TMARPL
Sbjct: 149 YLQ**KKYMG*LWHFMGGFEDDEPPSKRARASSVESTILSESFCYSKSINPLGGTMARPL 328
Query: 452 PSQGKEVMVGSKG 490
SQGKEVMVGSKG
Sbjct: 329 ASQGKEVMVGSKG 367
Score = 115 bits (246), Expect(2) = 6e-027
Identities = 47/61 (77%), Positives = 50/61 (81%)
Frame = +3 / +3
Query: 288 KNIWADLGISWEVLKMTSHPQNAQEHPQ*NLQVYQTVSRFRNFPILWEVQWLDLCLPKGK 467
KNIWAD GISWEVLKM +HPQNAQEHPQ*+ Q YQ VS RN LWEVQWLDL LP+GK
Sbjct: 165 KNIWADSGISWEVLKMMNHPQNAQEHPQ*SQQFYQKVSVIRNPLTLWEVQWLDLWLPRGK 344
Query: 468 K 470
K
Sbjct: 345 K 347
Score = 113 bits (242), Expect = 8e-025
Identities = 49/65 (75%), Positives = 53/65 (81%)
Frame = +1 / +1
Query: 289 KIYGLILAFHGRF*R*RATLKTRKNILSRICKFTRLFLVFEIFQSFGKYNG*TFAFPRER 468
KIYGL LAFHGRF*R* TLKTRK+ILSR+ F R FL+FEI FG+YNG*TF FP ER
Sbjct: 166 KIYGLTLAFHGRF*R**TTLKTRKSILSRVNNFIRKFLLFEIH*PFGRYNG*TFGFPGER 345
Query: 469 SYGWF 483
SYGWF
Sbjct: 346 SYGWF 360
Score = 111 bits (237), Expect = 4e-024
Identities = 46/78 (58%), Positives = 54/78 (69%)
Frame = -3 / -1
Query: 463 PLGRQRSSHCTSQRIGKFRKRETVW*TCRFY*GCSCAF*GWLVIFRTSHEMPRSAHIFFT 284
PLG QRSSHCTSQR+ FR ET * C Y*GCSCAF*GW +IF+TSHEMP SAHIFF
Sbjct: 340 PLGSQRSSHCTSQRVNGFRITETF**NC*LY*GCSCAF*GWFIIFKTSHEMPESAHIFFL 161
Query: 283 SGKVMVCNLHTNCCKQLP 230
++ C +++P
Sbjct: 160 LLQIQCSVSCRKCRQRIP 107
Score = 102 bits (217), Expect = 2e-021
Identities = 47/65 (72%), Positives = 50/65 (76%)
Frame = -2 / -3
Query: 482 NQP*LLSLGKAKV*PLYFPKDWKISKTRNSLVNLQILLRMFLRVLRVARHLQNLP*NAKI 303
NQP*LLS GK KV*PLY PK IS RN L+ L LLRM LRVLRV HLQNLP*NA++
Sbjct: 359 NQP*LLSPGKPKV*PLYLPKG*WISNNRNFLIKLLTLLRMLLRVLRVVHHLQNLP*NARV 180
Query: 302 SPYIF 288
SPYIF
Sbjct: 179 SPYIF 165
Score = 26.3 bits (51), Expect(3) = 2e-034
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = -3 / -3
Query: 241 KQLPKQIEDSSAGSPDPR*FAEKPREEPRIRA 146
++L K +S G DPR*FA E R R+
Sbjct: 140 RKLQKMPPKNSVGCSDPR*FARGTPESARKRS 45
Score = 24.9 bits (48), Expect(3) = 2e-034
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -2 / -1
Query: 158 PNPRRISRESPAPAVG 111
PNPR RESPA A G
Sbjct: 67 PNPRGNVRESPAGAGG 20
Score = 24.9 bits (48), Expect(2) = 6e-027
Identities = 10/15 (66%), Positives = 12/15 (80%)
Frame = +2 / +1
Query: 119 PGRAIPWRFGADSGL 163
P +AIP RF ADSG+
Sbjct: 28 PPQAIPERFRADSGV 72
Score = 24.0 bits (46), Expect(3) = 3e-031
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = +1 / +3
Query: 112 PTAGAGDSLEIRRGFG 159
P A AGDS RGFG
Sbjct: 21 PPAPAGDSRTFPRGFG 68
Score = 22.1 bits (42), Expect(3) = 3e-031
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +3 / +1
Query: 150 RIRGSSRGFSANQRGSGLPAE 212
R R S ANQRGS P E
Sbjct: 49 RFRADSGVPRANQRGSEQPTE 111
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 340,845,705
Number of Sequences: 312970
Number of extensions: 6455253
Number of successful extensions: 169955
Number of sequences better than 1.0e-020: 2
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 7083590403
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)